摘要：
目的观察针刺内关、足三里等穴对胃扩张模型大鼠孤束核(Nucleus of the Solitary Tract,NTS)c-fos表达及胃内压的影响,阐明NTS可能是内关、足三里穴共同调节胃功能的一个重要的延髓初级中枢。方法采用抗FOS蛋白的免疫组织化学技术和电生理技术。结果①造成大鼠胃扩张模型后,能显著诱导NTS内c—fos的表达,而电针足三里、内关穴组与胃扩张模型组比较,NTS内FOS阳性细胞
摘要：
目的：观察针刺内关、足三里等穴对胃扩张模型大鼠孤束核（nucleus of the solitary tract，NTS）神经元放电的影响。方法：运用细胞外记录神经电生理学方法，先找到大鼠NTS胃扩张相关神经元，以内关、足三里、偏历、合阳穴为刺激点作为研究对象，在各刺激点上分别用手针施以捻转手法刺激30s，记录NTS内胃扩张相关神经元的放电反应。结果：55只大鼠共记录到149个NTS神经元放电，149个N，I＇S神经元中与胃扩张相关的有65个，其中，44个对胃扩张刺激呈增频反应，21个呈减频反应。65个NTS胃扩张相关神经元中对来自体表刺激有反应的神经元出现比例。在针刺足三里、内关、偏历、合阳穴后分别为61．54％、53．85％、29．23％、33．85％。对来自体表的刺激，NTS内胃扩张相关神经元主要是以兴奋为主。选取44个对胃扩张刺激呈增频反应的神经元，比较针刺足三里、内关、偏历、合阳穴前后神经元频率变化，其兴奋性神经元频率变化率分别为（34．61±6．88）％、（31．73＋8．05）％、（19．84＋4．50）％、（18．07＋4．29）％，内关、足三里穴组之间无显著性差异（P〉0．05），而内关、足三里穴组与偏历、合阳穴组比较均有显著性差异（P〈0．01）。结论：NTS是针刺和内脏（胃）伤害性刺激传人的共同的信息整合中枢；腧穴具有相对特异性。
通讯机构：
School of Acu-moxibustion and Massage, Hunan University of Chinese Medicine, Changsha 410208, China.,
关键词：
急性心肌缺血;电针;内关;足三里;孤束核;c-fos表达
摘要：
目的:观察针刺"内关""足三里"对心肌缺血模型大鼠孤束核(nucleus of the solitary tract,NTS)c-fos表达及心电图ST_Ⅱ的影响,阐明延髓初级中枢在"内关""足三里"穴共同调节心功能中的作用.方法:将36只雄性SD大鼠随机分为生理盐水组、模型组、足三里组、内关组、偏历组及合阳组,每组6只,腹腔注射异丙肾上腺素造成心肌缺血模型.电针治疗20 min,采用抗Fos蛋白免疫组织化学技术观察大鼠NTS内c-fos表达和电生理技术观察大鼠心电图ST_Ⅱ的变化.结果:心肌缺血模型大鼠NTS内c-foS的表达明显增加(P＜0.01),而足三里组、内关组与模型组比较,NTS内c-foS阳性细胞数均明显减少(P＜0.01);足三里组、内关组NTS内c-foS阳性细胞数显著低于偏历组、合阳组(P＜0.01).足三里组、内关组大鼠缺血性心电图ST_Ⅱ电位值显著降低(P＜0.01),其电位降低值大于偏历组、合阳组(P＜0.01).结论:NTS是针刺内关、足三里共同调节心功能的整合中枢之一.
摘要：
AIM: To explore the mechanism of the exciting effects of electro-acupuncture (EA) at Sibai on the gastric myoelectric activities. METHODS: A total of 32 rats were randomly divided into four groups. Through intraperitoneal injection with atropine (the anti-cholinergic agent by blockade of muscarinic receptors), hexamethonium (automatic nerve ganglion-blocking agent) and reserpine (anti-adrenergic agent by depleting the adrenergic nerve terminal of its norepinephrine store), effects of EA at Sibai on the gastric myoelectric activities of the denervated rats were observed. RESULTS: After intraperitoneal injection of atropine and hexamethonium, the average amplitude and ratio of period to time in the phase of high activity of gastric myoelectric slow wave, and the average numbers of the peaks of gastric myoelectric fast wave were significantly decreased (P < 0.01, P < 0.05, P < 0.01), while after intraperitoneal injection of reserpine, the aforementioned three parameters were increased (P < 0.01, P < 0.05, P < 0.01). EA at Sibai point partially relieved the inhibitory effect of atropine and hexamethonium on the gastric myoelectric activities in the rats (P < 0.05 or P > 0.05). CONCLUSION: Cholinergic and adrenergic nervous systems and autonomic nerve ganglion participate in the peripheral passage of the controlling effects of EA at Foot Yangming Channel on gastrointestinal tract. (C) 2006 The WJG Press. All rights reserved.
摘要：
心胃相关是指心与胃在生理病理上相互联系、相互影响的关系,心胃相关理论体现了中医的整体观、辩证观.深入探讨内脏感觉的初级传入中枢一孤束核(Nucleus of the Solitary Tract,NTS)在心胃相关中的作用,可为心胃相关理论提供现代神经生物学依据,为中医基础理论的现代化研究提供一定的思路和方法.
