作者机构：
[杨岩涛; 周晋; 石继连; 刘文龙; 贺福元] College of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China;[吴春英] College of Humanity and Information Management, Hunan University of Chinese Medicine, Changsha 410208, China
期刊：
Journal of Thermal Analysis and Calorimetry,2012年109(1):395-402 ISSN：1388-6150
通讯作者：
Xiao, Xiaohe
作者机构：
[Dai, Bing; Li, Xingfeng] Hunan Univ Tradit Chinese Med, Coll Pharm, Changsha 410208, Hunan, Peoples R China.;[Zhou, Jian; Yan, Dan; Wang, Jiabo; Xiao, Xiaohe; Zhao, Yanling; He, Jing; Jin, Cheng] 302 Mil Hosp China, China Mil Inst Chinese Mat Med, Beijing 100039, Peoples R China.;[Wang, Haitao] 306 Mil Hosp, Dept Pharm, Beijing 100101, Peoples R China.;[Xiao, Xiaohe] 302 Mil Hosp China, China Mil Inst Chinese Mat Med, 100 W 4th Ring Middle Rd, Beijing 100039, Peoples R China.
通讯机构：
[Xiao, Xiaohe] 3;302 Mil Hosp China, China Mil Inst Chinese Mat Med, 100 W 4th Ring Middle Rd, Beijing 100039, Peoples R China.
摘要：
Using microcalorimetry, the characteristic metabolic heat flow power-time curves of S. aureus growth affected by Ursodesoxycholic acid and Hyodeoxycholic acid were measured at 37 °C. The thermal-kinetic parameters such as, growth rate constant k, the maximum power output (P
m), the time corresponding to the maximum power output (t
p), total heat-production Q
t
, half inhibitory concentration of the drugs (IC
50) were calculated from the growth curves. For both HDCA and UDCA, with the increasing of concentration, k, P
m, and Q
t
decreased, meanwhile, k–c fit a linear equation, t
p was prolonged correspondingly. Principle component analysis, the results indicated t
p might be the main parameter in evaluating the antibacterial activity of HDCA and UDCA in microcalorimetric method. Combining with t
p and IC
50, the results revealed that the differences and trends of antibacterial activity of these bile acid derivatives were: HDCA > UDCA. Structure-activity relationship (SAR) analysis showed that the α-OH at C-3 and C-6 position at equal pace on the steroid nucleus enhanced the hydrophilicity of HDCA, which led to a stronger antibacterial effect than UDCA. In this study, a useful tool was provided to accurately evaluate the antibacterial effects of bile acid derivatives. The thermolysis curve recorded by microcalorimetry could provide a lot of kinetic and thermodynamic information for the study of growth process of living microbial, which could be helpful in the screening of high efficacy antibacterial agents.
作者：
Ye, Tao;Jin, Cheng*;Zhou, Jian;Li, Xingfeng;Wang, Haitao;...
期刊：
Journal of Pharmaceutical and Biomedical Analysis,2011年55(5):1239-1244 ISSN：0731-7085
通讯作者：
Jin, Cheng
作者机构：
[Zhou, Jian; Xiao, Xiaohe; Ye, Tao; Jin, Cheng; Li, Xingfeng] 302 Mil Hosp, Intergrat Med Ctr, China Mil Inst Chinese Meteria Med, Beijing 100039, Peoples R China.;[Zhou, Jian; Ye, Tao] Jiangxi Univ TCM, Coll Pharm, Nanchang 330004, Peoples R China.;[Li, Xingfeng] Hunan Univ TCM, Coll Pharm, Changsha 410208, Hunan, Peoples R China.;[Wang, Haitao] 306 Mil Hosp, Beijing 100101, Peoples R China.;[Yang, Ying; Deng, Pingye; Wu, Yanwen] Beijing Ctr Phys & Chem Anal, Beijing 100089, Peoples R China.
通讯机构：
[Jin, Cheng] 3;302 Mil Hosp, Intergrat Med Ctr, China Mil Inst Chinese Meteria Med, Beijing 100039, Peoples R China.
关键词：
Musk;Electronic nose;Chemometrics;Principal component analysis;Quality control
摘要：
Musk is a precious and wide applied material in traditional Chinese medicine, also, an important material for the perfume industry all over the world. To establish a rapid, cost-effective and relatively objective assessment for the quality of musk, different musk samples, including authentic, fake and adulterate, were collected. A oxide sensor based electronic nose (E-nose) was employed to measure the musk samples, the E-nose generated data were analyzed by principal component analysis (PCA), the responses of 18 sensors of E-nose were evaluated by loading analysis. Results showed that a rapid evaluation of complex response of the samples could be obtained, in combination with PCA and the perception level of the E-nose was given better results in the recognition values of the musk aroma. The authentic, fake and adulterate musk could be distinguished by E-nose coupled with PCA, sensor 2, 3, 5, 12, 15 and 17 were found to be able to better discriminate between musk samples, confirming the potential application of an electronic instrument coupled with chemometrics for a rapid and on-line quality control for the traditional medicines.
