摘要：
The molecular and cellular mechanisms behind the involvement of inflammation in melanoma have not been fully elucidated. In this study, knockdown of Hmgb1 expression increased apoptosis, reduced invasion and p-NF-kappa B expression, but increased Klotho protein level in melanoma tumor cells. The effect of Hmgb1 knockdown was overcome by LPS. Introduction of exogenous Hmgb1 significantly decreased apoptosis, increased invasion, elevated p-NF-kappa B, but lowered Klotho protein level in melanoma cells. The effect of exogenous Hmgb1 was agonized by NF-kappa B inhibitor CAPE. Hmgb1 knockdown activated, but exogenous Hmgb1 inactivated, p-IGF1R/p-PI3K p-85/p-Akt/p-mTOR signaling. Knockdown of Klotho gene expression significantly decreased apoptosis, increased invasion in melanoma cells, and inhibited xenograft A375 tumor growth. A significantly high percentage of cells stained positive for p-NF-kappa B, but negative for Klotho, in melanoma tissues compared to normal and benign skin tissues. The positive p-NF-kappa B and negative Klotho protein expression correlated with poor prognosis in melanoma patients. Multivariate analysis revealed an independent association between p-NF-kappa B / Klotho protein level and overall survival. In conclusion, Hmgb1 can inhibit Klotho gene expression and malignant phenotype in melanoma cells through activation of NF-kappa B signaling.
摘要：
Abstract In this study, the complete nucleotide sequence of Belgium Malinois mitochondrial genome was determined for the first time. Sequence analysis showed that the genome structure was in accordance with other dogs. It contained 22 tRNA genes, 2 ribosomal RNA genes, 13 protein-coding genes and 1 control region (D-loop region). The base composition was A (31.7%), G (14.1%), C (25.5%) and T (28.7%), so the percentage of A and T (60.4%) was higher than that of G and C. The complete mitochondrial genome sequence of the Belgium Malinois will shed a new light on further dog breeds study.
摘要：
A series of isoquinoline alkaloids including tetrahydroprotoberberines, N-methyl tetrahydroprotoberberines and protoberberines were facile synthesised with protopines as the starting material. All compounds were evaluated for their antibacterial activities against four pathogenic bacteria Escherichia coli, Staphyloccocus aureus, Staphylococcus gallinarum and Salmonella choleraesuis. Experimental results indicated that protoberberines were the most active compounds to the target bacteria among the tested alkaloids. It was suggested that planar molecule with high aromatisation level (e.g. coptisine 5 and berberine 6) or a positive charge of the molecules (e.g. N-methyl tetrahydroprotoberberines 11 and 12) had a positive influence on the antibacterial effects.
通讯机构：
[Li, Shun-xiang] H;Hunan Univ Chinese Med, Sch Pharm, Changsha 410208, Hunan, Peoples R China.
摘要：
This study aimed to investigate the composition of three major stilbenes (mulberroside A, oxyresveratrol, and resveratrol) in different portions of mulberries collected in different seasons and their change molds during growth by high-performance liquid chromatography. Mulberroside A levels were the highest in the bark and roots of Morus atropurpurea Roxb, Morus alba Linn, and Morus latifolia Poir. Oxyresveratrol levels were the highest in roots and stem. Both of these high levels were in September. The amount of resveratrol was very low in all samples. In the stem, Morus latifolia Poir contained more mulberroside A than the other two mulberries. Mulberroside A was not detected in the leaves of the three mulberries. In Morus atropurpurea Roxb seedlings, the root tended to contain more of the three stilbenes than leaves. The temporal peaks of resveratrol were always ahead of those for oxyresveratrol. The levels of the stilbenes varied in different portions of the varieties of mulberries collected in different season and in the seedlings of Morus atropurpurea Roxb.
作者机构：
[周晋] School of Bioscience and Technology, Hunan Agricultural University, Changsha 410128, China;[李顺祥; 季梅; 黄丹; 虢小翊] School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China;Key Lab. of Chinese Materia Medica Research and Development, Hunan Academy of Chinese Medicine, Changsha 410013, China;[颜新培] Sercultural Sciences Institute of Hunan Province, Changsha 410127, China;[郑群怡] Natsource Chemicals (Changsha) Corporation, Changsha 410329, China
通讯机构：
School of Bioscience and Technology, Hunan Agricultural University, China
