期刊论文

Hu, Jue（胡珏）;Lu, Fangguo（卢芳国）

英文

Microchimica Acta

0026-3672

2018

185

3

1-8

10.1007/s00604-018-2702-0

National Natural Science Foundation of Hunan provinceNatural Science Foundation of Hunan Province [2016JJ3098]; Education department of Hunan Province [15B169]; Hunan traditional Chinese medicine administration [201711]; Science and Technology Innovation Team in Colleges and Universities in Hunan Province [15, Grxjb-7]; Doctor start-up Foundation of Hunan University of Chinese Medicine [9982-1001019]; Hunan University of Chinese Medicine [1]; Hunan Provincial Level Course [48]

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The authors describe a method for the fluorometric determination of methicillin-resistant Staphylococcus aureus (MRSA) by exploiting target-triggered chain reactions and deoxyribonuclease I (DNase I)-aided target recycling. It is making use of a carboxy-fluorescein (FAM)-labeled single-stranded probe containing two sections. One is complementary to the 5' terminus of the target, while the 3' terminus of the other target is adsorbed on the surface of graphene oxide (GO) via pi-stacking interactions without the target (16S rRNA). This adsorption results in quenching of the fluorescence of the la...