抗流感病毒性肺炎的有效中药复方筛选及机制研究
期刊：
中国免疫学杂志,2018年34(8):1168-1173 ISSN：1000-484X
作者机构：
湖南中医药大学中医学院, 长沙, 410208;湖南中医药大学中西结合学院, 长沙, 410208;湖南中医药大学医学院, 长沙, 410208;[李玲; 欧阳建军; 廖灿] 湖南中医药大学中医学院, 长沙, 410208;[胡珏; 魏科; 卢芳国] 湖南中医药大学医学院, 长沙, 410208
关键词：
病毒性肺炎;中药复方;免疫抑制剂;麻杏石甘汤;细胞因子
摘要：
目的：将免疫学原理应用于流感病毒性肺炎的制备,从而筛选出有效防治流感病毒性肺炎的中药复方,并进一步探讨该复方的作用机制。方法：（1）用浓度为75 mg/（kg·d）的免疫抑制剂短暂抑制小鼠免疫功能再进行A型流感病毒滴鼻感染,实验设多组中药复方组,同步设奥司他韦组、模型组以及正常组,灌胃给予相应的临床等效剂量药物7 d后,处理动物,进行指标检测,筛选出有效中药复方。（2）建立流感病毒性肺炎模型,实验设麻杏石甘汤的低、中、高剂量组,奥司他韦组、模型组和正常组,灌胃给予相应临床等效剂量药物7 d后,处理动物,进行指标检测。结果：（1）麻杏石甘汤、小柴胡汤、葛根汤,银翘散等中药复方中对小鼠肺炎均有一定的疗效,其中以麻杏石甘汤最为突出,可显著减轻肺部炎症等。（2）麻杏石甘汤可以改善小鼠的一般状况、肺部炎症和调控细胞因子的表达,上调脾指数,其中麻杏石甘汤中、高剂量组总体治疗效果更为显著。结论：免疫抑制的流感病毒性肺炎小鼠模型在筛选抗流感病毒性肺炎的中药复方中具有较好的实验研究价值,筛选出来的麻杏石甘汤作为有效的抗流感病毒性肺炎的中药复方,其可能的作用机制是通过提高机体的免疫功能,调节细胞因子的表达和分泌,从而减轻肺部炎症,改善小鼠的一般状况。
语种：
中文
展开
Graphene oxide-based fluorometric determination of methicillin-resistant Staphylococcus aureus by using target-triggered chain reaction and deoxyribonuclease-assisted recycling
作者：
Ning, Yi
（宁毅）;Zou, Li;Gao, Qiang
（高强）;Hu, Jue
*（胡珏）;Lu, Fangguo
（卢芳国） 期刊：
Microchimica Acta,2018年185(3):1-8 ISSN：0026-3672
作者机构：
[Ning, Yi; Zou, Li; Gao, Qiang; Hu, Jue; Lu, Fangguo; Lu, FG] Hunan Univ Chinese Med, Dept Microbiol, Sch Med, Changsha 410208, Hunan, Peoples R China.;[Lu, Fangguo; Lu, FG] Univ Innovat Team Hunan Prov, Key Discipline Pathogen Biol, Changsha 410208, Hunan, Peoples R China.
通讯机构：
[Hu, J; Lu, FG ] H;Hunan Univ Chinese Med, Dept Microbiol, Sch Med, Changsha 410208, Hunan, Peoples R China.;Univ Innovat Team Hunan Prov, Key Discipline Pathogen Biol, Changsha 410208, Hunan, Peoples R China.
关键词：
deoxyribonuclease I;DNA;graphite;oxide;RNA 16S;chemistry;genetic procedures;genetics;isolation and purification;limit of detection;methicillin resistant Staphylococcus aureus;nucleic acid hybridization;nucleotide sequence;procedures;spectrofluorometry;Base Sequence;Biosensing Techniques;Deoxyribonuclease I;DNA;Graphite;Limit of Detection;Methicillin-Resistant Staphylococcus aureus;Nucleic Acid Hybridization;Oxides;RNA, Ribosomal, 16S;Spectrometry, Fluorescence
摘要：
The authors describe a method for the fluorometric determination of methicillin-resistant Staphylococcus aureus (MRSA) by exploiting target-triggered chain reactions and deoxyribonuclease I (DNase I)-aided target recycling. It is making use of a carboxy-fluorescein (FAM)-labeled single-stranded probe containing two sections. One is complementary to the 5′ terminus of the target, while the 3′ terminus of the other target is adsorbed on the surface of graphene oxide (GO) via π-stacking interactions without the target (16S rRNA). This adsorption results in quenching of the fluorescence of the label and protects it from being cleaved by DNase I. However, upon addition of the target, DNA/RNA hybrids are repelled by GO. This leads to fluorescence recovery as measured at excitation/emission wavelengths of 480/514nm due to a chain reaction that is triggered by the target. The signal is strongly amplified by using DNase I-mediated target recycling. The 16S rRNA of MRSA can be detected by this method in the 1 to 30nM concentration range, and the detection limit is 0.02nM. The method was applied to analyze bacterial samples, and the detection limit is as low as 30 CFU. mL−1. The assay is highly sensitive and selective and in our percpetion has a large potential in diagnosis of drug-resistant bacteria. [Figure not available: see fulltext.]. © 2018, Springer-Verlag GmbH Austria, part of Springer Nature.
语种：
英文
展开
