期刊：
Journal of Biological Chemistry,2013年288(51):36733-36740 ISSN：0021-9258
通讯作者：
Liao, Duan-Fang
作者机构：
[Bu, Yiwen; Luo, Dixian; Rajput, Sandeep; Cao, Deliang] So Illinois Univ, Simmons Canc Inst, Dept Med Microbiol Immunol & Cell Biol, Sch Med, Springfield, IL 62794 USA.;[Luo, Dixian] First Peoples Hosp Chenzhou, Inst Translat Med, Chenzhou 423000, Hunan, Peoples R China.;[Luo, Dixian] First Peoples Hosp Chenzhou, Dept Lab Med, Chenzhou 423000, Hunan, Peoples R China.;[Ma, Jun; Cao, Deliang] Med Coll Guangdong, Affiliated Shenzhen Futian Hosp, Shenzhen 518033, Peoples R China.;[Ma, Jun; Cao, Deliang] Shenzhen Third Peoples Hosp, Guangdong Med Coll, Shenzhen 518112, Peoples R China.
通讯机构：
[Liao, Duan-Fang] H;Hunan Univ Chinese Med, State Key Lab Chinese Med Powder & Med Innovat Hu, Div Stem Cell Regulat & Applicat, Changsha 410208, Hunan, Peoples R China.
关键词：
Breast Cancer;Chaperone;HSP90;Lysosomes;Protein Secretion;Tumor Marker
摘要：
Background: Aldo-keto reductase 1B10 (AKR1B10) protein is a new tumor biomarker in humans. Results: Heat shock protein 90 (HSP90) is a chaperone molecule that mediates transportation to lysosomes and secretion of AKR1B10. Helix 10 of AKR1B10 protein mediates its interaction with HSP90. Conclusion: HSP90 mediates AKR1B10 secretion through binding to its helix 10 domain. Significance: This finding is significant in exploiting the use of AKR1B10 in cancer clinics. Aldo-keto reductase 1B10 (AKR1B10) protein is a new tumor biomarker in humans. Our previous studies have shown that AKR1B10 is secreted through a lysosome-mediated nonclassical pathway, leading to an increase in the serum of breast cancer patients. This study illuminates the regulatory mechanism of AKR1B10 secretion. The cytosolic AKR1B10 associates with and is translocated to lysosomes by heat shock protein 90 (HSP90), a chaperone molecule. Ectopic expression of HSP90 significantly increased the secretion of endogenous AKR1B10 and exogenous GFP-AKR1B10 fusion protein when cotransfected. Geldanamycin, a HSP90 inhibitor, dissociated AKR1B10-HSP90 complexes and significantly reduced AKR1B10 secretion in a dose-dependent manner. We characterized the functional domain in AKR1B10 and found that helix 10 (amino acids 233-240), located at the C terminus, regulates AKR1B10 secretion. Targeted point mutations recognized that amino acids Lys-233, Glu-236, and Lys-240 in helix 10 mediate the interaction of AKR1B10 with HSP90. Together, our data suggest that HSP90 mediates AKR1B10 secretion through binding to its helix 10 domain. This finding is significant in exploiting the use of AKR1B10 in cancer clinics.
期刊：
Materials Science and Engineering C: Materials for Biological Applications,2013年33(7):4416-4426 ISSN：0928-4931
通讯作者：
Wang, Jiali
作者机构：
[Wang, Jue; Tang, Jian; Wang, Jiali; Qin, Ling; Yue, Ye] Chinese Acad Sci, Inst Biomed & Hlth Engn, Ctr Translat Med Res & Dev, Shenzhen 518055, Peoples R China.;[Wang, Jiali; Qin, Ling] Chinese Univ Hong Kong, Dept Orthopaed & Traumatol, Musculoskeletal Res Lab, Hong Kong, Hong Kong, Peoples R China.;[Wang, Kai] Hunan Univ Chinese Med, Sch Humanities & Social Sci, Changsha 410208, Hunan, Peoples R China.;[Li, Weirong; Li, Yangde] Guangdong Innovat Team Biodegradable Magnesium &, Eande 523660, Dongguan, Peoples R China.
通讯机构：
[Wang, Jiali] C;Chinese Univ Hong Kong, Dept Orthopaed & Traumatol, Musculoskeletal Res Lab, Hong Kong, Hong Kong, Peoples R China.
关键词：
Magnesium and its alloys;In vitro;Absorbable;Cytotoxicity;Internal fixation
摘要：
Magnesium alloys have been advocated as potential artificial bone materials due to their biocompatibility and biodegradability. The understanding of their corrosive mechanism in physiological environments is therefore essential for making application-orientated designs. Thus, this in vitro study was designed to assess the effects of CO2 on corrosive behavior of AZ31D to mimic in vivo special ingredient. Electrochemical technologies accompanied with Scanning electron microscope, Fourier transform infrared, X-ray diffraction, Energy dispersive spectroscopy and hydrogen evolution measurement were employed to analyze corrosive rates and mechanisms of AZ31D. Moreover, the biocompatibility of AZ31D was assessed with a direct cell attachment assay and an indirect cytotoxicity test in different diluted extracts. The ion concentrations in extracts were measured using inductively coupled plasma mass spectrometry to offer explanations on the differences of cell viability in the indirect test. The results of the direct cytotoxicity assay showed that the corrosive rate of AZ31D was too rapid to allow for cell adhesion. Extracts diluted less than 20 times would cause adverse effects on cell proliferation, likely due to excessive ions and gas release. Moreover, the presence of CO2 did not cause significant differences on corrosive behavior of AZ31D according to the results of electrochemical testing and hydrogen evolution measurement. This might be caused by the simultaneous process of precipitation and dissolution of MgCO3 due to the penetration role of CO2. This analysis of corrosive atmospheres on the degradation behavior of magnesium alloys would contribute to the design of more scientific in vitro testing systems in the future. (C) 2013 The Authors. Published by Elsevier B.V. All rights reserved.
期刊：
Chemical Physics Letters,2012年527:73-78 ISSN：0009-2614
通讯作者：
Huang, Ying
作者机构：
[Huang, Ying; Liu, Lianghong] Hunan Univ Chinese Med, Sch Pharm, Changsha 410208, Hunan, Peoples R China.;[Liu, Shubin] Univ N Carolina, Ctr Res Comp, Chapel Hill, NC 27599 USA.
通讯机构：
[Huang, Ying] H;Hunan Univ Chinese Med, Sch Pharm, Changsha 410208, Hunan, Peoples R China.
作者机构：
[Wei Xing; Wang Dong-sheng; Wan Ling; Li Ji-yang; Zhong Guang-wei] Cent S Univ, Xiangya Hosp, Changsha 410008, Hunan, Peoples R China.;[Wang Zhan-yi] Henan Yuzhou Chinese Med Hosp, Yuzhou 461670, Peoples R China.;[Duan Xiao-peng; He Fu-yuan] Hunan Tradit Chinese Med Univ, Changsha 410208, Hunan, Peoples R China.;[Jiang Yao] Cent S Univ, State Key Lab Powder Met, Changsha 410083, Peoples R China.
通讯机构：
[Wang Dong-sheng] C;Cent S Univ, Xiangya Hosp, Changsha 410008, Hunan, Peoples R China.
关键词：
Ti-Al intermetallic compound porous materials;Dahuangzhecong Fang;arterial thrombosis;isolated Chinese medicine
摘要：
The mechanism of antithrombotic of Dahuangzhechong Fang separated and purified by Ti-Al intermetallic compound porous material (TAICPM) was researched. Dahuangzhechong Fang, which was isolated and screened by TAICPM, was used to oral rats. At the end of study, their blood and thrombus were collected. The results show that TAICPM with the pore size of 1-5 μm can screen Dahuangzhechong Fang well. Dahuangzhechong Fang can increase 6-keto-PGF1α, lower content of TXD2 and platelet. Dahuangzhechong Fang has good effect to resist arterial thrombosis.
作者机构：
[张颖杰; 张旭; 曹宝成] School of Stomatology, Lanzhou University, Lanzhou 730000, China;[王育华] School of Physical Science and Technology, Lanzhou University, Lanzhou 730000, China;[李娜] School of Stomatology, Northwest University for Nationalities, Lanzhou 730000, China
通讯机构：
School of Stomatology, Lanzhou University, China
期刊：
Proceeding of 2012 International Conference on Information Management, Innovation Management and Industrial Engineering, ICIII 2012,2012年1:352-356
通讯作者：
Du, Y.(710599369@qq.com)
作者机构：
[Du Ying] Business School, Hunan University of TCM, CSU, Changsha, China;[Luo Xinxing] Business School, Central South University, Changsha, China
会议名称：
International Conference on Information Management, Innovation
会议时间：
2012-10-20
会议地点：
Sanya(CN)
会议论文集名称：
2012 International conference on information management, innovation management and industrial engineering. v.1
摘要：
This paper focuses on the effect of the venom of the scorpion Buthus martensii on the proliferation of human cervical carcinoma Hela cell line and the related molecular mechanism. MTT test showed that
期刊：
Journal of Thermal Analysis and Calorimetry,2012年109(1):395-402 ISSN：1388-6150
通讯作者：
Xiao, Xiaohe
作者机构：
[Dai, Bing; Li, Xingfeng] Hunan Univ Tradit Chinese Med, Coll Pharm, Changsha 410208, Hunan, Peoples R China.;[Zhou, Jian; Yan, Dan; Wang, Jiabo; Xiao, Xiaohe; Zhao, Yanling; He, Jing; Jin, Cheng] 302 Mil Hosp China, China Mil Inst Chinese Mat Med, Beijing 100039, Peoples R China.;[Wang, Haitao] 306 Mil Hosp, Dept Pharm, Beijing 100101, Peoples R China.;[Xiao, Xiaohe] 302 Mil Hosp China, China Mil Inst Chinese Mat Med, 100 W 4th Ring Middle Rd, Beijing 100039, Peoples R China.
通讯机构：
[Xiao, Xiaohe] 3;302 Mil Hosp China, China Mil Inst Chinese Mat Med, 100 W 4th Ring Middle Rd, Beijing 100039, Peoples R China.
作者机构：
[Chen, Yinzhuang; Xu, Guangming; Yao, Shouzhuo] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410081, Hunan, Peoples R China.;[Chen, Bo; Wang, Hua] Hunan Normal Univ, Key Lab Chem Biol & Tradit Chinese Med Res, Minist Educ China, Changsha 410081, Hunan, Peoples R China.;[Xu, Guangming] Hunan Univ Chinese Med, Coll Pharmaceut Sci, Changsha 410208, Hunan, Peoples R China.;[Ding, Li] Shimadzu Res Lab Shanghai Co Ltd, Shanghai, Peoples R China.
通讯机构：
[Chen, Bo] H;Hunan Normal Univ, Key Lab Chem Biol & Tradit Chinese Med Res, Minist Educ China, Changsha 410081, Hunan, Peoples R China.
摘要：
Desorption corona beam ionization mass spectrometry, a novel direct MS analysis technique, was first employed to probe intermediates in Leuckart reaction between ketone and ammonium formate at 185 °C to corroborate the reaction mechanism.
摘要：
The objective of this study was to apply the "on/off" switch consisting of 3' phosphorothioate-modified allele specific primers and exo(+) polymerase in single base discrimination of A1555G and C1494T mutations in the highly conserved sites of the mitochondrial 12S rRNA. The two point mutations are the hotspot mutations associated with either aminoglycoside antibiotics induced deafness or inherited nonsyndromic hearing loss. The PCR products of mitochondrial DNA (mtDNA) 12S rRNA gene were inserted into the pMD19-T vector for transformation into Escherichia coli JM109 competent cells for preparing wild-type pMD19-T/mt vector. Inverse PCR was carried out for mtDNA 12S rRNA gene C1494T and A1555G mutagenesis and DpnI endonuclease degradating methylated pMD19-T/mt vector existing in the inverse PCR products was carried out to construct the mutation-type pMD19-T/mtM vector. These constructed vectors were confirmed by DNA sequencing. Allelic specific primers targeting wild-type and mutation-type templates were designed with 3' terminal phosphorothioate modification. Two-directional primer extension was performed using Pfu polymerases. Amplified by exo(+) polymerase, allelic specific primers perfectly matching wild-type allele were extended while no products were produced from primers targeting point-mutated deafness-related allele. Similarly, allelic specific primers perfectly matching point-mutated deafness-related mutation-type allele were extended and no products were yielded from primers targeting wild-type allele. No specific product was observed in the primer extension reaction mediated by on/off switch in screening the mtDNA 12S rRNA gene harboring either C1494T or A1555G mutation in 40 healthy volunteers tested. These data suggest that the "off switch" mediated by exo(+) polymerase is highly reliable in the diagnosis of monogenic diseases and the novel "on/off" switch has enormous applications in systematic and extended screening of the12S rRNA gene A1555G and C1494T mutations. The established assay can be widely used not only for hearing loss patients but also for normal subjects before the use of aminoglycoside antibiotics.
摘要：
Using microcalorimetry, the characteristic metabolic heat flow power-time curves of S. aureus growth affected by Ursodesoxycholic acid and Hyodeoxycholic acid were measured at 37 °C. The thermal-kinetic parameters such as, growth rate constant k, the maximum power output (P
m), the time corresponding to the maximum power output (t
p), total heat-production Q
t
, half inhibitory concentration of the drugs (IC
50) were calculated from the growth curves. For both HDCA and UDCA, with the increasing of concentration, k, P
m, and Q
t
decreased, meanwhile, k–c fit a linear equation, t
p was prolonged correspondingly. Principle component analysis, the results indicated t
p might be the main parameter in evaluating the antibacterial activity of HDCA and UDCA in microcalorimetric method. Combining with t
p and IC
50, the results revealed that the differences and trends of antibacterial activity of these bile acid derivatives were: HDCA > UDCA. Structure-activity relationship (SAR) analysis showed that the α-OH at C-3 and C-6 position at equal pace on the steroid nucleus enhanced the hydrophilicity of HDCA, which led to a stronger antibacterial effect than UDCA. In this study, a useful tool was provided to accurately evaluate the antibacterial effects of bile acid derivatives. The thermolysis curve recorded by microcalorimetry could provide a lot of kinetic and thermodynamic information for the study of growth process of living microbial, which could be helpful in the screening of high efficacy antibacterial agents.
摘要：
Organometallic Lewis acids play an important role in modern organic synthesis. How to design and synthesize highly efficient and recyclable organometallic Lewis acid catalysts that can be conveniently applied in chemical reactions are key issues for sustainable synthetic processes. In general, stronger acidity means higher catalytic activity for organometallic Lewis acids. However, with the rise in acidity, the compound becomes more susceptible to hydrolysis and cannot be recycled. Simultaneous improvement of the hygroscopic character and Lewis acidity/catalytic activity of organometallic Lewis acids is highly desirable from the standpoint of practical applications. In this mini-review, the history of air-stable organometallic Lewis acids is introduced, with emphasis on our research works on metallocene, organobismuth, and organoantimony Lewis acids to the aspects of synthesis, characterization and catalytic application in carbon–carbon bond (Friedel–Crafts acylation, Mukaiyama aldol reactions; allylation, cyclotrimerization, Mannich reactions, cross-condensation reactions) and carbon–heteroatom bond (acylation, S–S bond cleavage, glycosylation) formation reactions. In terms of stability, storage, versatile ability, high catalytic activity and chemo-/stereo-selectivity, the complexes will find broad applications in organic synthesis.
作者机构：
[翁柳娜; 邹方卉; 刘向前; 郑礼胜; 李丽丽] School of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China;[刘向前] School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China
通讯机构：
School of Chemistry and Chemical Engineering, Central South University, China
