摘要：
Background: QiShenYiQi Pills (R) (QSYQ) is a compound Chinese medicine used in China for alleviating cardiac function. The present study was designed to explore the effect and mechanism of QSYQ on ischemia-reperfusion (I/R)-induced disorders in myocardial structure and function, with particularly focusing on the regulation of energy metabolism. Methods: Sprague-Dawley rats, with or without QSYQ pretreatment, were subjected to 30 min occlusion of the left anterior descending coronary artery and followed by 90 min or 24 h reperfusion. Myocardial blood flow (MBF) and cardiac function were evaluated at baseline, immediately after ischemia and 30, 60, 90 min, and 24 h after reperfusion. Myocardial infarction, myocardial histology and ultrastructure were assessed. Double staining of alpha-cardiac actinin and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling was conducted to assess myocardial apoptosis. ATP, ADP and AMP content was determined by Enzyme-Linked Immunosorbent Assay, F-actin in myocardial cells determined by immunofluorescence microscopy and expression of ATP synthase alpha, ATP5D, and phosphorylated-Myosin Light Chain (P-MLC) determined by western blotting. Results: Pre-treatment with QSYQ protected against I/R-induced MBF decrease, myocardial infarction and apoptosis at 90 min and 24 h after reperfusion. Moreover, I/R 90 min caused an impairment on cardiac function, a decrease in the ratio of ADP/ATP and AMP/ATP, accompanying with reduction of ATP 5D expression and increase in the expression of P-MLC, meanwhile, myocardium to exhibit myocardial fiber rupture, interstitial edema, and mitochondria swelling, all of which were significantly ameliorated by pre-treatment with QSYQ. Conclusions: The results of the present study suggest an involvement of regulation of energy metabolism in the action of QSYQ to protect against myocardial I/R injury. (C) 2012 Elsevier Ireland Ltd. All rights reserved.
期刊：
International Journal of Cancer,2013年132(2):496-497 ISSN：0020-7136
通讯作者：
Cao, Deliang
作者机构：
[Cao, Deliang] So Illinois Univ, Sch Med, Simmons Canc Inst, Dept Med Microbiol Immunol & Cell Biol, Springfield, IL 62794 USA.;[Liao, Duan-Fang] Hunan Univ Chinese Med, Div Stem Cell Regulat & Applicat, State Key Lab Chinese Med Powder & Med Innovat Hu, Changsha, Hunan, Peoples R China.;[Cao, Deliang] So Illinois Univ, Sch Med, Simmons Canc Inst, Dept Med Microbiol Immunol & Cell Biol, 913 N Rutledge St, Springfield, IL 62794 USA.
通讯机构：
[Cao, Deliang] S;So Illinois Univ, Sch Med, Simmons Canc Inst, Dept Med Microbiol Immunol & Cell Biol, 913 N Rutledge St, Springfield, IL 62794 USA.
摘要：
The International Journal of Value-Based Management presents papers which clarify the role of values in organizational behavior and in the process of decision making. The Journal's main purpose is to keep readers abreast of developments and new techniques in all areas of management. To this end, case studies relating to specific companies, products, and industries are included, as are empirical studies, especially those adopting a comparative cultural viewpoint. Papers exploring the relationships between personal/organizational values and performance are published, as are comparative management studies offering specific ramifications for the practising manager within a particular culture. The International Journal of Value-Based Management aimes at an international readership, it provides indispensable reading for the modern manager and business professional, management scientist, business school faculty and their students at every level, and all those who seek to understand the impact that values and ethics have on the modern business.Coverage in the Journals@Ovid database begins with the Volume 10, Issue 1, 1997 issue.
通讯机构：
[Cai, Guangxian] H;Hunan Univ Tradit Chinese Med, Key Lab Internal Med, Changsha 410007, Hunan, Peoples R China.
关键词：
neural regeneration;traditional Chinese medicine;Buyang Huanwu Decoction;cerebral ischemia;nestin;BrdU;microtubule-associated protein-2;growth-associated protein 43;neural stem cells;proliferation;differentiation;cerebral cortex;subventricular
摘要：
The traditional Chinese medicine Buyang Huanwu Decoction has been shown to improve the neurological function of patients with stroke. However, the precise mechanisms underlying its effect remain poorly understood. In this study, we established a rat model of cerebral ischemia by middle cerebral artery occlusion and intragastrically administered 5 g/kg Buyang Huanwu Decoction, once per day, for 1, 7, 14 and 28 days after cerebral ischemia. Immunohistochemical staining revealed a number of cells positive for the neural stem cell marker nestin in the cerebral cortex, the subventricular zone and the ipsilateral hippocampal dentate gyrus in rat models of cerebral ischemia. Buyang Huanwu Decoction significantly increased the number of cells positive for 5-bromodeoxyuridine (BrdU), a cell proliferation-related marker, microtubule-associated protein-2, a marker of neuronal differentiation, and growth-associated protein 43, a marker of synaptic plasticity in the ischemic rat cerebral regions. The number of positive cells peaked at 14 and 28 days after intragastric administration of Buyang Huanwu Decoction. These findings suggest that Buyang Huanwu Decoction can promote the proliferation and differentiation of neural stem cells and enhance synaptic plasticity in ischemic rat brain tissue.
摘要：
<正>Aim:To explore the protective effects of Genistein Sulfonate Sodium(GSS) on brain ischemia/reperfusion(I/R) injury and its mechanism about cholinergic anti-inflammatory pathway.Methods:The rats wer
摘要：
Essential hypertension (EH) is a frequent, chronic, age-related disorder, which remains a major modifiable risk factor for cardiovascular disease despite important advances in our understanding of its pathophysiology. Previous studies have noted a consistent maternal effect on blood pressure (BP). Consequently, mutations in mitochondrial DNA (mtDNA) have become an additional target of investigations on the missing BP heritability. Among these mutations, mt-transfer RNA (tRNA) is a hot mutational spot for pathogenic mutations associated with EH. Mutant mtDNA aggravates mitochondrial dysfunction, pivotally contributing to the clinical phenotype. Moreover, the damaged mitochondria, due to their inability to provide the high-energy requirements for cells, generate reactive oxygen species (ROS) and induce mitochondrial-mediated cell death pathways. Therefore, mitochondrial dysfunction plays a critical role in the pathogenesis of EH. This review summarizes the basic knowledge of mitochondrial genetics and EH-associated mtDNA mutations and further discusses the molecular mechanisms behind these mtDNA mutations in clinical manifestations of EH.
作者机构：
Hunan Tradit Chinese Med Univ, Hunan Prov Peoples Hosp 2, Dept Urol, Changsha, Hunan, Peoples R China.;[Yang, Jin-Rui] Cent S Univ, Xiangya Hosp 2, Dept Urol, Changsha 410011, Hunan, Peoples R China.
通讯机构：
[Yang, Jin-Rui] C;Cent S Univ, Xiangya Hosp 2, Dept Urol, Changsha 410011, Hunan, Peoples R China.
摘要：
OBJECTIVE To prospectively evaluate perioperative results and 12-month follow-up after plasmakinetic enucleation of the prostate (PKEP) and transvesical open prostatectomy (OP) for benign prostatic hyperplasia (BPH) > 80 mL. METHODS A total of 83 patients with a prostate > 80 mL were randomized to either PKEP or OP. Perioperative and postoperative outcome data were obtained during a 12-month follow-up. RESULTS No statistical differences were observed in the preoperative data. Both groups resulted in a similar and significant postoperative improvement in International Prostate Symptom Score (IPSS), quality of life (QOL), maximum uroflow rate (Qmax), postvoid residual (PVR) urine volume and prostate specific antigen (PSA), but no significant difference was found between the groups at the 12-month follow-up. Compared to OP, operation time (111.2 +/- 27.1 minutes vs 109.6 +/- 28.2 minutes, P = .708) were not significantly different between the groups, but blood loss was significantly less (10.2 +/- 4.5 g/l vs 15.1 +/- 4.3 g/l, P < .001), and bladder irrigation (2.4 +/- 1.0 days vs 4.3 +/- 1.1 days, P < .001), catheterization time (3.3 +/- 1.1 days vs 6.2 +/- 1.3 days, P < .001), and hospital stay (5.4 +/- 1.2 days vs 9.3 +/- 1.1 days, P < .001) were significantly shorter in the PKEP group. Effects on erectile function were similar in both groups, but adverse events were less frequent in the PKEP group. CONCLUSION PKEP can be performed safely and is an equally effective procedure for treatment of large BPH with OP, with minimal complications and faster postoperative recovery. The PKEP helps to reduce the morbidity associated with OP and may become the attractive alternative to OP for patients with large BPH. UROLOGY 82: 176-181, 2013. (C) 2013 Elsevier Inc.
期刊：
Journal of Biological Chemistry,2013年288(51):36733-36740 ISSN：0021-9258
通讯作者：
Liao, Duan-Fang
作者机构：
[Bu, Yiwen; Luo, Dixian; Rajput, Sandeep; Cao, Deliang] So Illinois Univ, Simmons Canc Inst, Dept Med Microbiol Immunol & Cell Biol, Sch Med, Springfield, IL 62794 USA.;[Luo, Dixian] First Peoples Hosp Chenzhou, Inst Translat Med, Chenzhou 423000, Hunan, Peoples R China.;[Luo, Dixian] First Peoples Hosp Chenzhou, Dept Lab Med, Chenzhou 423000, Hunan, Peoples R China.;[Ma, Jun; Cao, Deliang] Med Coll Guangdong, Affiliated Shenzhen Futian Hosp, Shenzhen 518033, Peoples R China.;[Ma, Jun; Cao, Deliang] Shenzhen Third Peoples Hosp, Guangdong Med Coll, Shenzhen 518112, Peoples R China.
通讯机构：
[Liao, Duan-Fang] H;Hunan Univ Chinese Med, State Key Lab Chinese Med Powder & Med Innovat Hu, Div Stem Cell Regulat & Applicat, Changsha 410208, Hunan, Peoples R China.
关键词：
Breast Cancer;Chaperone;HSP90;Lysosomes;Protein Secretion;Tumor Marker
摘要：
Background: Aldo-keto reductase 1B10 (AKR1B10) protein is a new tumor biomarker in humans. Results: Heat shock protein 90 (HSP90) is a chaperone molecule that mediates transportation to lysosomes and secretion of AKR1B10. Helix 10 of AKR1B10 protein mediates its interaction with HSP90. Conclusion: HSP90 mediates AKR1B10 secretion through binding to its helix 10 domain. Significance: This finding is significant in exploiting the use of AKR1B10 in cancer clinics. Aldo-keto reductase 1B10 (AKR1B10) protein is a new tumor biomarker in humans. Our previous studies have shown that AKR1B10 is secreted through a lysosome-mediated nonclassical pathway, leading to an increase in the serum of breast cancer patients. This study illuminates the regulatory mechanism of AKR1B10 secretion. The cytosolic AKR1B10 associates with and is translocated to lysosomes by heat shock protein 90 (HSP90), a chaperone molecule. Ectopic expression of HSP90 significantly increased the secretion of endogenous AKR1B10 and exogenous GFP-AKR1B10 fusion protein when cotransfected. Geldanamycin, a HSP90 inhibitor, dissociated AKR1B10-HSP90 complexes and significantly reduced AKR1B10 secretion in a dose-dependent manner. We characterized the functional domain in AKR1B10 and found that helix 10 (amino acids 233-240), located at the C terminus, regulates AKR1B10 secretion. Targeted point mutations recognized that amino acids Lys-233, Glu-236, and Lys-240 in helix 10 mediate the interaction of AKR1B10 with HSP90. Together, our data suggest that HSP90 mediates AKR1B10 secretion through binding to its helix 10 domain. This finding is significant in exploiting the use of AKR1B10 in cancer clinics.
摘要：
Background: In recent years, the brain gut axis theory has received increasing attention in studies of depression. However, most studies separately address potential antidepressant and prokinetic treatments. Investigations of drugs that could potentially treat comorbid depression and gastrointestinal (GI) dysfunction via a common mechanism of action have not yet been performed in detail. Aim: To find a common mechanism of action of our patented drug, meranzin hydrate (MH), in the antidepressant and prokinetic treatment. Methods: The forced swimming test (FST) model of depression, plasma ghrelin measurement, and in vivo and in vitro measurements of GI motility were used. Results: 1. Administration of MH (9 mg/kg) decreased the immobility time during the FST after acute treatment; this effect was inhibited by the alpha 2-adrenoceptor antagonist, yohimbine, but not by the alpha 1-adrenoceptor antagonist, prazosin. 2. After chronic treatment, the immobility time of rats during the FST was decreased significantly by MH (2.25 mg/kg). 3. MH (9 mg/kg) increased plasma ghrelin levels in rats subjected to the FST; this increase was enhanced by the ghrelin receptor agonist, GHRP-6. 4. MH (9 mg/kg) also promoted gastric emptying and intestinal transit in rats with or without FST. 5. In vitro, MH (10 mu M) increased jejunal contractions in rats subjected to the FST; this effect was inhibited by yohimbine. Furthermore, the inhibitory effect of yohimbine was partly reversed by the ghrelin receptor agonist, GHRP-6. Conclusion: Our study revealed that MH from natural resources exhibits antidepressive and prokinetic-like effects through the regulation of the common mediator, the alpha 2-adrenoceptor. (C) 2012 Elsevier Ltd. All rights reserved.
期刊：
Materials Science and Engineering C: Materials for Biological Applications,2013年33(7):4416-4426 ISSN：0928-4931
通讯作者：
Wang, Jiali
作者机构：
[Wang, Jue; Tang, Jian; Wang, Jiali; Qin, Ling; Yue, Ye] Chinese Acad Sci, Inst Biomed & Hlth Engn, Ctr Translat Med Res & Dev, Shenzhen 518055, Peoples R China.;[Wang, Jiali; Qin, Ling] Chinese Univ Hong Kong, Dept Orthopaed & Traumatol, Musculoskeletal Res Lab, Hong Kong, Hong Kong, Peoples R China.;[Wang, Kai] Hunan Univ Chinese Med, Sch Humanities & Social Sci, Changsha 410208, Hunan, Peoples R China.;[Li, Weirong; Li, Yangde] Guangdong Innovat Team Biodegradable Magnesium &, Eande 523660, Dongguan, Peoples R China.
通讯机构：
[Wang, Jiali] C;Chinese Univ Hong Kong, Dept Orthopaed & Traumatol, Musculoskeletal Res Lab, Hong Kong, Hong Kong, Peoples R China.
关键词：
Magnesium and its alloys;In vitro;Absorbable;Cytotoxicity;Internal fixation
摘要：
Magnesium alloys have been advocated as potential artificial bone materials due to their biocompatibility and biodegradability. The understanding of their corrosive mechanism in physiological environments is therefore essential for making application-orientated designs. Thus, this in vitro study was designed to assess the effects of CO2 on corrosive behavior of AZ31D to mimic in vivo special ingredient. Electrochemical technologies accompanied with Scanning electron microscope, Fourier transform infrared, X-ray diffraction, Energy dispersive spectroscopy and hydrogen evolution measurement were employed to analyze corrosive rates and mechanisms of AZ31D. Moreover, the biocompatibility of AZ31D was assessed with a direct cell attachment assay and an indirect cytotoxicity test in different diluted extracts. The ion concentrations in extracts were measured using inductively coupled plasma mass spectrometry to offer explanations on the differences of cell viability in the indirect test. The results of the direct cytotoxicity assay showed that the corrosive rate of AZ31D was too rapid to allow for cell adhesion. Extracts diluted less than 20 times would cause adverse effects on cell proliferation, likely due to excessive ions and gas release. Moreover, the presence of CO2 did not cause significant differences on corrosive behavior of AZ31D according to the results of electrochemical testing and hydrogen evolution measurement. This might be caused by the simultaneous process of precipitation and dissolution of MgCO3 due to the penetration role of CO2. This analysis of corrosive atmospheres on the degradation behavior of magnesium alloys would contribute to the design of more scientific in vitro testing systems in the future. (C) 2013 The Authors. Published by Elsevier B.V. All rights reserved.
期刊：
Frontiers of Computer Science,2013年7(3):307-316 ISSN：2095-2228
作者机构：
湖南大学
关键词：
AES;RSA;ECC;signed-digit number;FPGA;cryptographic algorithms;high radix;arithmetic unit
摘要：
Recently, security in embedded system arises attentions because of modern electronic devices need cautiously either exchange or communicate with the sensitive data. Although security is classical research topic in worldwide communication, the researchers still face the problems of how to deal with these resource constraint devices and enhance the features of assurance and certification. Therefore, some computations of cryptographic algorithms are built on hardware platforms, such as field program gate arrays (FPGAs). The commonly used cryptographic algorithms for digital signature algorithm (DSA) are rivest-shamir-adleman (RSA) and elliptic curve cryptosystems (ECC) which based on the presumed difficulty of factoring large integers and the algebraic structure of elliptic curves over finite fields. Usually, RSA is computed over GF(p), and ECC is computed over GF(p) or GF(2p). Moreover, embedded applications need advance encryption standard (AES) algorithms to process encryption and decryption procedures. In order to reuse the hardware resources and meet the trade-off between area and performance, we proposed a new triple functional arithmetic unit for computing high radix RSA and ECC operations over GF(p) and GF(2p), which also can be extended to support AES operations. A new high radix signed digital (SD) adder has been proposed to eliminate the carry propagations over GF(p). The proposed unified design took up 28.7% less hardware resources than implementing RSA, ECC, and AES individually, and the experimental results show that our proposed architecture can achieve 141.8MHz using approximately 5.5k CLBs on Virtex-5 FPGA.
