期刊：
Frontiers in Medicine,2022年9:2800 ISSN：2296-858X
作者机构：
[Nie, Duorui; Xu, Fei] Hunan Univ Chinese Med, Coll Pharm, Changsha, Peoples R China.;[Nie, Duorui] Hunan Univ Chinese Med, Grad Sch, Changsha, Peoples R China.;[Lan, Qingxia; Shi, Bin] Guangzhou Univ Chinese Med, Clin Med Coll 1, Guangzhou, Peoples R China.;[Xu, Fei] Hunan Engn Technol Res Ctr Bioact Subst Discovery, Changsha, Peoples R China.;[Xu, Fei] Hunan Prov Sino US Int Joint Res Ctr Therapeut Dru, Changsha, Peoples R China.
摘要：
BackgroundDue to underrepresentation in randomized controlled trials among old people (>= 65 years old), the effectiveness of clinical trial-based recommendations about the treatment for stage I pancreatic cancer remains controversial. In this research, we intended to investigate the different strategies of this population in surgery group and non-surgery group. Materials and methodsElderly patients aged 65 years or older with histologically diagnosed stage I pancreatic cancer from 2006 to 2017 were identified from the Surveillance, Epidemiology, and End Results (SEER) database. The included patients were divided into surgery group (receiving surgery with chemotherapy or chemoradiotherapy) and non-surgery group (receiving radiotherapy, chemotherapy, both, or neither). Overall survival (OS) and cancer-specific survival (CSS) were compared between groups by Kaplan-Meier analysis. Cox proportional hazards regression (Cox) proportional hazards regression was used to determine factors associated with survival. ResultsA total of 2,448 eligible patients were recruited. Among them, 18.4% were treated surgically and 81.6% were treated non-surgically. The median OS (mOS) was 26 months (95% CI: 24-30 months) in the surgery group and 7 months (95% CI: 7-8 months) in the non-surgery group. In multivariate analyses, surgery was an important factor in improving OS compared with non-surgical treatment (HR: 0.34, 95% CI: 0.29-0.39, p < 0.001). In subgroup analysis, surgery plus chemotherapy was an independent factor for OS in the surgery group, while chemoradiotherapy, chemotherapy, and radiotherapy were independent prognostic factors for patients in the non-surgery group. ConclusionSurgical resection and post-operative chemotherapy are recommended for elderly patients with stage I pancreatic cancer who can tolerate treatment, but post-operative chemoradiotherapy does not bring survival benefits compared with post-operative chemotherapy. Moreover, radiotherapy, chemotherapy, or the combination of radiotherapy and chemotherapy are significantly related to the prognosis of elderly patients with untreated pancreatic cancer, but chemoradiotherapy has the most obvious benefit.
摘要：
Industrial effluents are vital sources of environmental pollutants that cause many problems for human health. In recent years, the use of nanomaterials for the removal of contaminants from effluents has received much attention. Nanoadsorbents are one of the most important nanomaterials that have many benefits, including low-cost, high efficiency, easy synthesis, and process simplicity. Also, nanoadsorbents have high porosity, high specific surface area, and low surface-to-volume ratio compared to conventional adsorbents. Nanocatalysts are another important nanomaterial for removing contaminants from wastewater. In the present work, the appli-cation of nanotechnology (nanoadsorbents, nanocatalysts, and nanofiltration) were thoroughly studied in the elimination of contaminants from wastewater. Also, the advantages and disadvantages of nanoadsorbents, nanocatalysts, and nanofiltration membranes, various types of them, and their impacts on the environment were fully studied. Moreover, the reusability, stability, and mechanism of nanoadsorbents and nanocatalysts in the removal of contaminants were investigated. Besides, the challenges facing nanotechnology in wastewater treatment were discussed. According to previous studies, TiO2, ZnO, and Ni0.6Ca0.4Fe2O4 have shown significant photocatalytic efficiency in removing organic pollutants. Also, Fe3O4 nanoparticles, ZnS nanocrystals, MWCNTs, graphene oxide, and their composites with other materials have shown remarkable efficiency in wastewater treatment. Moreover, polysulfone@graphene oxide nanofiltration membrane was able to eliminate CR and MB dyes from water with a rejection efficiency of higher than 99%.
期刊：
Journal of Natural Products,2022年85(4):838-845 ISSN：0163-3864
通讯作者：
Lei, Li;Zeng, QH
作者机构：
[Zeng, QH; Hu, Yi-Bo; Zhang, Li; Lei, Li; Zhou, Ying; Jiang, Ling; Xiang, Hong; Fu, Chu-Han; Huang, Li-Hua; Yang, Si-Yu; Zeng, Qing-Hai; Yu, Xing; Huang, Jin-Hua; Lei, Xin-Xin; Chen, Jing] Cent South Univ, Xiangya Hosp 3, Dept Dermatol, Changsha 410013, Peoples R China.;[Wen, Xin-Yue; Zeng, Hong-Liang] Hunan Acad Chinese Med, Inst Chinese Mat Med, Changsha 410013, Peoples R China.
通讯机构：
[Zeng, QH ; Lei, L] C;Cent South Univ, Xiangya Hosp 3, Dept Dermatol, Changsha 410013, Peoples R China.
摘要：
Hyperpigmented skin diseases such as melasma, freckles, and melanosis usually mar the appearance of patients. Traditional herbal medicines are highly accepted in inhibiting skin pigmentation, with advantages of high efficiency, low cost, and low side effects. Selaginellin (SEL), one of the active compounds of selaginella, has been proved to be exhibit antineoplastic, antioxidant, antisenescence, and antiapoptosis activities. In this study, we found that SEL can inhibit melanogenesis in vitro and in vivo. A mechanism study found that SEL inhibits melanogenesis through inhibiting the mitogen-activated protein kinase (MAPK) signaling pathway, then down-regulating the expression of microphthalmia-associated transcription factor (MITF) and downstream genes tyrosinase (TYR) and tyrosinase-related protein 2 (TYRP2). UVB-activated paracrine function of fibroblasts and keratinocytes promotes melanogenesis of melanocytes. Interestingly, SEL antagonizes UVB-activated paracrine function of fibroblasts and keratinocytes. These findings indicate that SEL can be a potential whitening compound to inhibit melanogenesis.
期刊：
Frontiers in Nutrition,2022年9:1008514 ISSN：2296-861X
作者机构：
[Chen, Shuai; Li, Yang; Long, Jiaxin; Wang, Jinlong] Univ Chinese Acad Sci, Hangzhou Inst Adv Study, Sch Life Sci, Key Lab Syst Hlth Sci Zhejiang Prov, Hangzhou, Peoples R China.;[Long, Jiaxin] Hunan Univ Chinese Med, Coll Pharm, Changsha, Peoples R China.
关键词：
gut microbiota;microbial metabolites;ischemic stroke;oxidative stress;inflammation;apoptosis
摘要：
Gut microbiota is increasingly recognized to affect host health and disease, including ischemic stroke (IS). Here, we systematically review the current understanding linking gut microbiota as well as the associated metabolites to the pathogenesis of IS (e.g., oxidative stress, apoptosis, and neuroinflammation). Of relevance, we highlight that the implications of gut microbiota-dependent intervention could be harnessed in orchestrating IS.
作者机构：
[Yao, Kunpeng; Liu, Qili; Zhang, Daoping; Chen, Xinyu] Hunan Univ Chinese Med, Changsha, Hunan, Peoples R China.;[Chen, Qingyang; Cai, Huzhi; Chen, Xinyu] Hunan Univ Chinese Med, Affiliated Hosp 1, Changsha, Hunan, Peoples R China.;[Wang, Yating; Cheng, Shuo] Beijing Univ Chinese Med, Sch Chinese Med, Beijing, Peoples R China.
通讯机构：
The First Affiliated Hospital, Hunan University of Chinese Medicine, Hunan, Changsha, China
关键词：
Resumen Objetivo: se utilizaron métodos bioinformáticos y técnicas de acoplamiento molecular para predecir los componentes efectivos, los objetivos y las vías biológicas relacionadas de la cápsula Xiexin en la intervención de la dislipidemia y explorar su mecanismo. Métodos: los componentes activos y los objetivos de la cápsula Xiexin fueron seleccionados por la base de datos TCMSP. Se utilizaron las plataformas Genecards, OMIM, PharmGkb, TTD (Therapeutic Target Database) y Drugbank para buscar las dianas de la enfermedad en la dislipidemia. El diagrama reticular “componente-diana” fue construido por el software Cytoscape 3.7.0, y la interacción proteína-proteína (PPI) fue analizada por la plataforma STRING. Los análisis de enriquecimiento de Gene Ontology (GO) y Kyoto Encyclopedia of Genes and Genomics (KEGG) se realizaron mediante paquetes de datos en lenguaje R para predecir el mecanismo de acción. El software AutoDockVina y PyMol se utilizó para unir los componentes activos clave de la cápsula Xiexin y las proteínas clave de la PPI. Resultados: se seleccionaron 65 componentes activos y 114 dianas. Veintitrés compuestos activos clave fueron seleccionados a partir de la tabla “componentes farmacéuticos-dianas”. Las redes PPI incluyen principalmente proteínas básicas como PTGS2, PTGS1 y HSP90AA1. Los resultados del análisis de enriquecimiento de GO y KEGG en los objetivos comunes se refieren principalmente a la vía de señalización mediada por esteroides, la respuesta hormonal esteroidea, el transporte y metabolismo lipídicos, la regulación del almacenamiento de colesterol, la vía de la ciclooxigenasa y otras vías biológicas, así como la vía de señalización de PPAR, la vía de señalización de IL-17, la vía de señalización de PI3K-Akt, la vía de señalización de FcnnnnnnnnRI y otras vías relacionadas. La prueba de acoplamiento molecular mostró que la quercetina se une mejor a la proteína diana central HSP90AA1, que es la proteína diana con la mejor actividad de unión de los componentes químicos clave de la cápsula Xiexin. Conclusión: los principales componentes químicos de la cápsula Xiexin pueden participar en la regulación de la PPAR y otras vías de señalización mediante la regulación de genes clave como ESR1, MAPK14 (mitogen-activated protein kinase 14), HSP90AA1, por lo que pueden desempeñar un papel farmacológico en la intervención de la dislipidemia. Palabras clave: Cápsula Xiexin;Dislipidemia;Bioinformática;Mecanismo molecular
摘要：
Objective: bioinformatic methods and molecular docking technology were used to predict the active components, targets, and related biological pathways of the Xiexin capsule in the intervention for dyslipidemia, exploring its mechanism. Methods: the active components and targets of the Xiexin capsule were screened by the TCMSP (Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform )database. Genecards (The Human Gene Database), OMIM (Online Mendelian Inheritance in Man), PharmGkb (Pharmacogenomics Knowledge Base database), TTD (Therapeutic Target Database), and Drugbank platforms were used to search the disease targets of dyslipidemia. The Cytoscape 3.8.0 software was used to construct the 'component-target' network diagram, and the STRING (functional protein association networks) platform was used to analyze protein-protein interaction (PPI). Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomics (KEGG) enrichment analyses were performed by R language data packets to predict the mechanism of action. The AutoDockVina and PyMol software were used to dock the key active components in the Xiexin capsule and the core proteins in PPI. Results: a total of 66 effective components were screened, involving 114 targets; 87 key active compounds were screened from the 'drug-component-target' diagram. The PPI network mainly involved core proteins such as PTGS2 (prostaglandin-endoperoxide synthase 2), PTGS1 (prostaglandin-endoperoxide synthase 1), and HSP90AA1 (heat shock protein 90 alpha family class A member 1). GO and KEGG enrichment analysis results of common targets mainly involved hormone-mediated signaling pathway, steroid hormone response, lipid transport and metabolism, regulation of cholesterol storage, cyclooxygenase pathway, and other biological pathways, as well asMM PPAR (peroxisome proliferators-activated receptor) signaling pathway, IL-17 (interleukin 17) signaling pathway, PI3K-Akt (protein kinase b) signaling pathway, FcεRI signaling pathway, and other related pathways. Molecular docking verification showed that quercetin had the best binding with the core target protein HSP90AA1, and HSP90AA1 was the target protein with the best binding activity for the key chemical components in Xiexin capsules. Conclusion: the main chemical components in the Xiexin capsules may participate in the regulation of PPAR and other signaling pathways by regulating key genes such as ESR1 (estrogen receptor 1), MAPK14 (mitogen-activated protein kinase 14), and HSP90AA1, to exert the pharmacological effect of the intervention on dyslipidemia.
摘要：
Background: Diabetes is a common chronic disease which has caused a great burden on families and society. The treatment of diabetes has always been a hotspot. This study aimed to explore the effect and mechanism of miR-30d-5pon inflammation of high glucose-impaired human keloid fibroblasts (HKF). Methods: Differently-expressed miRNAs were predicted by bioinformatics methods. Exosomes were observed by transmission electron microscope. Exosome particle sizes were measured by NanoSight. Western Blot was used to detect the expression of CD81, CD63, CD9, and Calnexin. QRT-PCR was used to detect the expression of miR-30d-5p, IL-1 beta, TNF-alpha, VEGF, FGF21, NRF2, and HO-1. The levels of IL-1 beta, TNF-alpha, IL-6, IL-10, and TGF-beta were determined by ELISA. Cell apoptosis and CD86, CD206 positive cells were detected by flow cytometry. Results: Tori formula could promote the secretion of endothelial progenitor cell (EPCs) exosomes. EPCs exosomes and miR-30d-5p could stimulate the proliferation of HKF impaired by high glucose and the expression of IL-10 and TGF-beta. MiR-30d-5p inhibited the proliferation of M1 macrophages and the expression of IL-1 beta and TNF-alpha. It could also promote the proliferation of M2 macrophages and the expression of CCL17 and CCL22. Moreover, miR-30d-5p stimulated the expression of VEGF, FGF21, NRF2, and HO-1, as well as suppressed the expression of IL-1 beta, TNF-alpha, and IL-6. MiR-30d-5p also restrained the apoptosis of impaired HKF. Conclusion: This study confirmed that miR-30d-5p could promote the M1/M2 polarization and inhibit the inflammatory response of impaired HKF, which provided a certain idea and direction for treating diabetes.
摘要：
A pair of new sesquiterpene isomers containing a spiro heterocyclic skeleton, dothimes A (1) and B (2), together with six known compounds, quindoline (3), (S)-3-(3-indolyl)lactic acid methyl ester (4), dankasterone B (5), dibutyl phthalate (6), (1S,3R,4R,7S)-3,4-dihydroxy-alpha-bisabolol (7), and p-hydroxybenzaldehyde (8), were iso-lated from the plant-derived fungus Botryosphaeria dothidea. The structures of all isolated compounds were determined based on extensive spectroscopic analyses, including 1D/2D nuclear magnetic resonance (NMR), and high resolution electrospray ionization mass spectrometry (HRESIMS) data, as well as by comparison with literature reports. Compounds 1 and 2 exhibited inhibitory effects on lipopolysaccharide (LPS)-induced nitric oxide (NO) production with IC50 values of 63.66 and 58.29 mu M, respectively.
摘要：
Ovarian malignant growth is perhaps the most lethal disease in females. There are no exact biomarkers for the early determination of ovarian disease. We obtained a total of 12 gene clus-ters through WGCNA and studied the azure gene modules related to the lymphatic infiltration of ovarian cancer further. What's more, endurance investigation was utilized to decide three qualities connected with the by and large and infection-free endurance in ovarian disease patients, including GOGA8B [Hazard Ratio (HR) =1.53, p = 0.037, 95% CI: 1.05-2.24], LRRC26 (HR = 0.7, P = 0.045, 95% CI: 0.48-1.01), and CCDC114 (HR = 0.72, P = 0.042, 95% CI: 0.53-0.98). A prognostic risk score model was built to anticipate the endurance pace of patients at 1, 3, and 5 years, individually. The area under the receiver operating characteristic (ROC) curve (AUC) of the training set was 0.749, 0.764, and 0.784, respectively; the test AUC was 0.601, 0.623, and 0.709. Our review gives a point of view on significant possible biomarkers for the determination, anticipation, and therapy of IP: 182.75.148.10 On: Sat, 20 Aug 2022 10:40:39 ovarian malignant growth.
作者：
Wang Yan-fang;Deng Yan;Zhang Su-ying;Liu Dong;Luo Bin;...
期刊：
中国结合医学杂志,2022年28(12):1072-1080 ISSN：1672-0415
通讯作者：
Ai-jun Sun
作者机构：
[Ma Rui-lin; Wang Yan-fang; Deng Yan; Sun Ai-jun] Chinese Acad Med Sci & Peking Union Med Coll, Peking Union Med Coll Hosp, Dept Obstet & Gynecol, Beijing 100730, Peoples R China.;[Zhang Su-ying] Hunan Univ Chinese Med, Dept Obstet & Gynecol, Affiliated Hosp 2, Changsha 410001, Peoples R China.;[Luo Bin; Liu Dong] Sichuan Univ, West China Hosp 2, Key Lab Birth Defects & Related Dis Women & Child, Minist Educ, Chengdu 610041, Peoples R China.;[Wang Xue; Deng Miao] Hangzhou Womens Hosp, Dept Obstet & Gynecol, Hangzhou Matern & Child Hlth Care Hosp, Hangzhou 310008, Peoples R China.
通讯机构：
[Ai-jun Sun] D;Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China
关键词：
Chinese medicine;Buxue Yimu Pills;gynecological anemia;network pharmacology;multi-target mechanism
摘要：
OBJECTIVE: To compare the clinical efficacy and safety of oral administration of Buxue Yimu Pills (BYP, ), ferrous sulfate (FS), and the combination of BYP and FS on gynecological anemia, and investigate the mechanisms using network pharmacology. METHODS: A randomized, controlled, multi-center clinical trial was conducted. Totally 150 patients with hemoglobin of 70-110 g/L due to gynecological conditions were recruited and randomized (using the block randomization method) into Buxue Yimu Pills group (24 g/d), oral iron group (FS Tablets, 0.9 g/d), and combined treatment group (BYP, 24 g/d plus FS Tablets, 0.9 g/d), 50 patients in each group. At the enrollment and 4-week treatment, complete blood count, serum iron indexes were evaluated. Adverse events, liver and renal functions, as well as blood coagulation were observed. Network pharmacology was conducted to identify the active ingredients and explore the potential mechanisms of BYP. RESULTS: Ten (20%) and 7 (14%) participants discontinued the therapy due to gastrointestinal symptoms in oral iron and combination treatment groups. All 3 groups showed elevated hemoglobin. The patients in the iron group exhibited typically elevated in serum iron and ferritin and decreased in total iron-binding capacity. No change in iron indexes was observed in BYP group. The patients in the combination treatment group neither showed significant changes in serum ferritin nor total iron-binding capacity. No significant adverse reactions were observed in the BYP group. The network pharmacology identified 27 bioactive compounds and 145 targets of BYP on gynecological anemia. Biological processes and pathways including regulation of inflammation, hormone, angiogenesis and hemostasis, response to decreased oxygen levels, effects on myeloma cell, and response to metal ions were identified. CONCLUSION: BYP contributes to the practical improvement on gynecological anemia potentially through multi-target mechanisms and optimized iron re-distribution. (Trial registration: No. NCT03232554).
摘要：
Abstract: Cantharidin (CTD) is the major component of anticancer drugs obtained from Mylabris Cichorii and has a good inhibitory effect on several cancers, including hepatocellular carcinoma (HCC) and breast cancer. However, due to its toxicity, oral administration can cause various adverse reactions, limiting its clinical application. The aim of this work was to design glycyrrhetinic acid (GA)- and/or folate (FA)-modified solid lipid nanoparticles (SLNs) for the encapsulation of CTD to target HCC. Four CTD-loaded SLNs (cantharidin solid lipid nanoparticles (CSLNs), glycyrrhetinic acid-modified cantharidin solid lipid nanoparticles (GA-CSLNs), folate-modified cantharidin solid lipid nanoparticles (FA-CSLNs), and glycyrrhetinic acid and folate-modified cantharidin solid lipid nanoparticles (GA-FA-CSLNs)) were prepared by the emulsion ultrasonic dispersion method, and their physicochemical parameters were determined (particle size and distribution, morphology, zeta-potential, entrapment efficiency, drug loading, and hemolysis). Additionally, the antitumor activities of the four SLNs were evaluated comprehensively by tests for cytotoxicity, cell migration, cell cycle, apoptosis, cellular uptake, competition suppression assay, and in vivo tumor suppression assay. Four SLNs showed spherical shapes and mean diameters in the range of 75–110 nm with size dispersion (PDI) within the range of 0.19–0.50 and zeta-potential approximately –10 mV. The entrapment efficiency of CTD in SLNs was higher than 95% for all tested formulations, and no hemolysis was observed. Compared to GA-CSLNs or CSLNs, GA-FA-CSLNs and FA-CSLNs showed stronger cytotoxicity on hepatocellular carcinoma cells (HepG2), and the cytotoxicity of GA-FA-CSLNs on hepatocyte cells (L-02) was remarkably reduced compared with other formulations. GA-FA-CSLNs and FA-CSLNs also increased the inhibition of HepG2 cell migration, and FA-CSLNs had the highest apoptosis rate. The cell cycle results indicated that HepG2 cells were arrested mainly in the S phase and G2/M phase. Analysis of competition inhibition experiments showed that GA and FA ligands had targeted effects on HepG2 cells. The in vivo tumor inhibition experiment showed that GA-FA-CSLNs and FA-CSLNs had excellent tumor inhibition ability—their tumor inhibition rates were 96.46% and 89.92%, respectively. Our results indicate that GA-FA-CSLNs and FA-CSLNs have a promising future in the therapeutic intervention of HCC. Keywords: cantharidin; solid lipid nanoparticles; glycyrrhetinic acid; folate; antitumor; liver-targeting
作者机构：
[Liao, Duan-fang; Choudhary, M. Iqbal; Shehla, Nuzhat; Li, Bin; Wang, Wei; Atta-ur-Rahman; Cao, Liang; Jian, Yuqing] Hunan Univ Chinese Med, TCM & Ethnomed Innovat & Dev Int Lab, Acad Atta Ur Rahman Belt & Rd Tradit Med Res Ctr, Sch Pharm, Changsha, Peoples R China.;[Choudhary, M. Iqbal; Shehla, Nuzhat; Wang, Wei; Atta-ur-Rahman] Univ Karachi, HEJ Res Inst Chem, Int Ctr Chem & Biol Sci, Karachi, Pakistan.;[Zhao, Jianping; Khan, Ikhlas A.] Univ Mississippi, Natl Ctr Nat Prod Res, Pharmaceut Sci Res Inst, University, MS 38677 USA.
通讯机构：
[Choudhary, M. Iqbal] H;[Choudhary, M. Iqbal] U;Hunan Univ Chinese Med, TCM & Ethnomed Innovat & Dev Int Lab, Acad Atta Ur Rahman Belt & Rd Tradit Med Res Ctr, Sch Pharm, Changsha, Peoples R China.;Univ Karachi, HEJ Res Inst Chem, Int Ctr Chem & Biol Sci, Karachi, Pakistan.
摘要：
Kadsura heteroclita Roxb. Craib. (Schisandraceae), is a vine plant mainly distributed in southwest part of China. A new dibenzocyclooctadiene lignan, kadsulignan W (1), along with eleven known lignans (2–12) were isolated from chloroform soluble fraction of stems of Kadsura heteroclita. The structure of new lignan was elucidated by extensive spectroscopic techniques, namely one- and two-dimensional NMR spectroscopy, and HRESI-MS analysis. The absolute configuration of the biphenyl ring in the new dibenzocyclooctadiene lignan was discerned by circular dichroism (CD) spectroscopy. Antioxidative effects of these compounds were evaluated on human isolated neutrophils, and compounds 5, 8, 9, and 10 were found to be strongly active with the IC50 of 36.68, 34.41, 35.97, and 33.65 µM, respectively. Furthermore, compound 8 was also found to be cytotoxic against human gastric cancer cells (BGC 823), and human cervical cancer cell lines (HeLa) with the IC50 values of 11.0, and 23.8 µM, respectively.
作者机构：
[Qiu, Jingyue; Xiong, Meng; Zhong, Meiqi; Yu, Chang; Peng, Qinghua; Ren, Baoping; Song, Houpan] Hunan Univ Chinese Med, Hunan Prov Key Lab Diagnost Res Chinese Med, Changsha 410208, Hunan, Peoples R China.;[Qiu, Jingyue; Xiong, Meng; Zhong, Meiqi; Yu, Chang; Ren, Baoping; Zeng, Meiyan; Song, Houpan] Hunan Univ Chinese Med, Coll Tradit Chinese Med, Changsha 410208, Hunan, Peoples R China.;[Peng, Qinghua] Hunan Univ Chinese Med, Affiliated Hosp 1, Changsha 410007, Hunan, Peoples R China.;[Ou, Chen; Peng, Qinghua] Hunan Univ Chinese Med, Hunan Prov Key Lab Prevent & Treatment Ophthalmol, Changsha 410208, Hunan, Peoples R China.;[Zeng, Meiyan] Hunan Univ Chinese Med, Coll Tradit Chinese Med, 300 Xueshi Rd, Changsha 410208, Hunan, Peoples R China.
通讯机构：
[Meiyan Zeng] C;[Qinghua Peng] H;College of Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China<&wdkj&>Hunan Provincial Key Laboratory of Diagnostic Research in Chinese Medicine, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China<&wdkj&>The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China<&wdkj&>Hunan Provincial Key Laboratory for the Prevention and Treatment of Ophthalmology and Otolaryngology Diseases with TCM, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China
关键词：
Duodenal ulcer;HQJZT;Inflammation;NF-κB;STAT;Traditional Chinese Medicine
摘要：
Huang-Qi-Jian-Zhong-Tang (HQJZT) is a well-known traditional Chinese herbal formulation. This study aimed to investigate the duodenoprotective properties of HQJZT against Indomethacin (IND)-induced duodenal ulceration in rats, and the mechanisms involved, particularly through NF-kappa B and STAT signaling pathways. Our results showed that HQJZT completely protected the duodenal mucosa from ulceration caused by IND, as indicated by improved macroscopic and histological appearances. There was a significant decrease in ulcer index and microscopic score, an increase in villus height and crypt depth, and a normalization of the tissue architecture of the duodenum in rats following HQJZT treatment. Blood flow into the duodenal mucosa was significantly increased after HQJZT administration. HQJZT significantly increased PGE2 and NO levels in the duodenal mu-cosa. A significant reduction in the production of pro-inflammatory cytokines IL-1 beta, IL-6, and TNF-alpha was observed in the duodenal mucosa under treatment with HQJZT. Mechanistically, the administration of HQJZT significantly lowered the duodenal protein expression of inflammation-related genes, including p-NF-kappa B and p-I kappa B beta, compared with the ulcer control group. Furthermore, the STAT signaling pathway-related protein markers p-JAK and p-STAT were significantly reduced in the HQJZT (1.30 and 2.60 g/kg) groups. As a result of these findings, HQJZT alleviates duodenal mucosal ulcers caused by IND. A protective effect of HQJZT on duodenal ulcers is attributed to its ability to improve mucosal blood flow, stimulate the production of cytoprotective mediators, minimize proinflammatory cytokines, and block the activation of NF-kappa B and STAT signaling pathways.
摘要：
LJF and LF are commonly used in Chinese patent drugs. In the Chinese Pharmacopoeia, LJF and LF once belonged to the same source. However, since 2005, the two species have been listed separately. Therefore, they are often misused, and medicinal materials are indiscriminately put in their related prescriptions in China. In this work, firstly, we established a model for discriminating LJF and LF using ATR-FTIR combined with multivariate statistical analysis. The spectra data were further preprocessed and combined with spectral filter transformations and normalization methods. These pretreated data were used to establish pattern recognition models with PLS-DA, RF, and SVM. Results demonstrated that the RF model was the optimal model, and the overall classification accuracy for LJF and LF samples reached 98.86%. Then, the established model was applied in the discrimination of their related prescriptions. Interestingly, the results show good accuracy and applicability. The RF model for discriminating the related prescriptions containing LJF or LF had an accuracy of 100%. Our results suggest that this method is a rapid and effective tool for the successful discrimination of LJF and LF and their related prescriptions.
作者机构：
[Yang, Xiaoyan; Ding, Changsong; Huang, Qunfu; Liu, Jiajun] Hunan Univ Chinese Med, Sch Informat, Changsha 410208, Hunan, Peoples R China.;[Ding, Changsong] Hunan Univ Chinese Med, Big Data Anal Lab Tradit Chinese Med, Changsha 410208, Hunan, Peoples R China.
通讯机构：
[Changsong Ding] S;School of Informatics, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China<&wdkj&>Big Data Analysis Laboratory of Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China
关键词：
*Formula efficacy;*Graph convolutional networks;*Herbal properties;*Heterogeneous graph;*Traditional Chinese medicine
摘要：
Chinese herbal formulae are the heritage of traditional Chinese medicine (TCM) in treating diseases through thousands of years. The formula function is not just a simple herbal efficacy addition, but produces complex and nonlinear relationships between different herbs and their overall efficacy, which brings challenges to the formula efficacy analysis. In our study, we proposed a model called HPE-GCN that combines graph convolutional networks (GCN) with TCM-defined herbal properties (TCM-HPs) to predict formulae efficacy. In addition, to process the unstructured natural language in the formula text, we proposed a weighting calculation method related to herb frequency and the number of herbs in a formula called Formula-Herb dependence degree (FHDD), to assess the dependency degree of a formula with its herbs. In our research, 214 classic tonic formulae from ancient TCM books such as Synopsis of the Golden Chamber, Jingyue's Complete Works and the Golden Mirror of Medicin were collected as datasets. The performance of HPE-GCN on multi-classification of tonic formulae reached the best result compared with classic machine learning models, such as support vector machine, naive Bayes, logistic regression, gradient boosting decision tree, and K-nearest neighbors. The evaluated index Macro-Precision, Macro-Recall, Macro-F1 of HPE-GCN on the test set were 87.70%, 84.08% and 83.51% respectively, increased by 7.27%, 7.41% and 7.30% respectively from second best compared models. GCN has the advantage of low-dimensional feature expression for herbs and formulae, and is an effective analysis tool for TCM research. HPE-GCN integrates TCM-HPs and fits the complex nonlinear mapping relationship between TCM-HPs and formulae efficacy, which provides new ideas for related research.
摘要：
Diet is the most direct and rapid contributor to the gut microbiome. Oils and fats are important nutrients in the human body. The effects of lard or vegetable blend oil on gut microbiota were investigated. Kunming mice were given lard or vegetable blend oil for six weeks. Changes in microbiota composition and abundance in lard or vegetable blend oil diets were analyzed by 16S rRNA gene amplicon sequencing. Our study shows that the gut microbiota of mice changed significantly after ingestion of lard or vegetable blend oil. Lard may synergize with Coriobacteriaceae_UCG-002. Vegetable blend oil has synergistic effects with Akkermansia, Roseburia, and Enteractinococcus. Coriobacteriaceae_UCG-002 showed a significant negative correlation with Glycolysis/Gluconeogenesis. Roseburia was most strongly associated with Starch and sucrose metabolism. According to bacterial function prediction and correlation analysis, long-term consumption of lard or vegetable oil may affect glycolipid metabolism, but lard has a greater impact on human health and consequently host health.
期刊：
BioMed Research International,2022年2022 ISSN：2314-6133
通讯作者：
Cheng, S.
作者机构：
[He, Chunxiang; Li, Ze; Song, Zhenyan; Cheng, Shaowu; Li, Ping; Zhu, Xu; Yang, Miao; Yu, Wenjing] Hunan Univ Chinese Med, Key Lab Hunan Prov Integrated Tradit Chinese & We, Changsha 410208, Hunan, Peoples R China.;[Song, Zhenyan; Cheng, Shaowu; Li, Ping; Zhu, Xu; Xiao, Chen] Hunan Univ Chinese Med, Coll Integrated Tradit Chinese & Western Med, Changsha 410208, Hunan, Peoples R China.
通讯机构：
[Cheng, S.] K;Key Lab. of Hunan Prov. for Intgd. Traditional Chinese and W. Med. on Prev. and Treatm. of Cardio-Cerebr. Dis., Hunan University of Chinese Medicine, Hunan, Changsha, China
