通讯机构：
[Xu, GM ] H;Hunan Univ Chinese Med, Coll Pharm, Changsha 410208, Hunan, Peoples R China.
关键词：
Affinity-ultrafiltration (AUF) and high-performance – tandem mass spectrometry (HPLC-MS/MS);molecular docking;Polygonum amplexicaule radix;thrombin
摘要：
Polygonum Amplexicaule Radix is a traditional Chinese medicine for promoting blood circulation and removing blood stasis, which is often used to treat thrombotic diseases. However, its potential anticoagulant active ingredients have been unexplored. In this study, a method for rapid screening of anticoagulant active ingredients from P. amplexicaule by affinity-ultrafiltration (AUF) and high-performance - tandem mass spectrometry (HPLC-MS/MS) was established, which using thrombin as an affinity receptor target. 5 potential anti-thrombin active components were screened: gallic acid, procyanidin B2-3''O-gallate, 11-O-galloylbergenin, (-)-epicatechin gallate and di-galloyl-O-bergenin by AUF and HPLC-MS/MS. The inhibitory ability of these active ingredients to thrombin was confirmed by in vitro activity experiments and molecular docking, hence justifying that P. amplexicaule is a traditional Chinese medicine with excellent anticoagulation effects. Meanwhile, it has been shown that AUF-MS could be a way rapid screening for active substances.
摘要：
Ralstonia solanacearum, the causal agent of bacterial wilt, is a devastating plant pathogenic bacterium that infects more than 450 plant species. Until now, there has been no efficient control strategy against bacterial wilt. In this study, we screened a library of 100 plant-derived compounds for their antibacterial activity against R. solanacearum. Twelve compounds, including harmine, harmine hydrochloride, citral, vanillin, and vincamine, suppressed bacterial growth of R. solanacearum in liquid medium with an inhibition rate higher than 50%. Further focus on harmine revealed that the minimum inhibitory concentration of this compound is 120 mg/L. Treatment with 120 mg/L of harmine for 1 and 2 h killed more than 90% of bacteria. Harmine treatment suppressed the expression of the virulence-associated gene xpsR. Harmine also significantly inhibited biofilm formation by R. solanacearum at concentrations ranging from 20 mg/L to 60 mg/L. Furthermore, application of harmine effectively reduced bacterial wilt disease development in both tobacco and tomato plants. Collectively, our results demonstrate the great potential of plant-derived compounds as antibacterial agents against R. solanacearum, providing alternative ways for the efficient control of bacterial wilt.
期刊：
Frontiers in Pharmacology,2023年14:1225515 ISSN：1663-9812
通讯作者：
Li, J
作者机构：
[Li, Shun-Xiang; Huang, Yu-Ting; Wang, Zhi; Ma, Yuan; Li, Juan; Zhao, Li-Juan; Duan, Yan; Fang, Ai-Qing; Dai, Yu-Ping; Lu, Yu-Ting] Hunan Univ Chinese Med, Sch Pharm, Changsha, Peoples R China.;[Zhou, Qi-Zhi; Zhou, Yan-Hui] Hunan Amazing Grace Biotechnol Co Ltd, Changsha, Peoples R China.;[Li, Shun-Xiang; Li, Juan; Zhao, Li-Juan] Hunan Engn Technol Res Ctr Bioact Subst Discovery, Changsha, Peoples R China.;[Li, Shun-Xiang; Li, Juan; Zhao, Li-Juan] Med Res Ctr, Hunan Prov Sino US Int Joint Res Ctr Therapeut Dru, Changsha, Peoples R China.
通讯机构：
[Li, J ] H;Hunan Univ Chinese Med, Sch Pharm, Changsha, Peoples R China.;Hunan Engn Technol Res Ctr Bioact Subst Discovery, Changsha, Peoples R China.;Med Res Ctr, Hunan Prov Sino US Int Joint Res Ctr Therapeut Dru, Changsha, Peoples R China.
摘要：
Object: This research intended to probe the antibacterial effect and pharmacodynamic substances of Tea-Seed Oil (TSO) through the use of ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) analysis, network analysis, and molecular docking.Methods: The major chemical components in the methanol-extracted fractions of TSO were subjected to UPLC-Q-TOF-MS. Network pharmacology and molecular docking techniques were integrated to investigate the core components, targets, and potential mechanisms of action through which the TSO exert their antibacterial properties. To evaluate the inhibitory effects, the minimum inhibitory concentration and diameter of the bacteriostatic circle were calculated for the potential active ingredients and their equal ratios of combinatorial components (ERCC) against Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans. Moreover, the quantification of the active constituents within TSO was achieved through the utilization of high-performance liquid chromatography (HPLC).Results: The methanol-extracted fractions contained a total of 47 chemical components, predominantly consisting of unsaturated fatty acids and phenolic compounds. The network pharmacology analysis and molecular docking analysis revealed that various components, including gallocatechin, gallic acid, epigallocatechin, theophylline, chlorogenic acid, puerarin, and phlorizin, have the ability to interact with critical core targets such as serine/threonine protein kinase 1 (AKT1), epidermal growth factor receptor (EGFR), a monoclonal antibody to mitogen-activated protein kinase 14 (MAPK14), HSP90AA1, and estrogen receptor 1 (ESR1). Furthermore, these components can modulate the phosphatidylinositol-3-kinase protein kinase B (PI3K-AKT), estrogen, MAPK and interleukin 17 (IL-17) signaling pathways, hereby exerting antibacterial effects. In vitro validation trials have found that seven components, namely gallocatechin, gallic acid, epigallocatechin, theophylline, chlorogenic acid, puerarin, and phloretin, displayed substantial inhibitory effects on E. coli, S. aureus, P. aeruginosa, and C. albicans, and are typically present in tea oil, with a total content ranging from 15.87 & SIM;24.91 & mu;g & BULL;g-1.Conclusion: The outcomes of this investigation possess the possibility to expand our knowledge base concerning the utilization of TSO, furnish a theoretical framework for the exploration of antibacterial drugs and cosmetics derived from inherently occurring TSO, and establish a robust groundwork for the advancement and implementations of TOS products within clinical settings.
期刊：
Cell Death & Disease,2023年14(3):183 ISSN：2041-4889
通讯作者：
Bai, Y.;Zhang, Z.
作者机构：
[Wu, Wanzhou] Cent South Univ, Xiangya Hosp 3, Dept Cardiol, Changsha, Peoples R China.;[Chen, Alex F.; Wu, Wanzhou] Cent South Univ, Xiangya Hosp 3, Ctr Vasc Dis & Translat Med, Dept Cardiol, Changsha, Peoples R China.;[Wang, Yue; Liao, Longsheng; Zhu, Lingping; Wu, Wanzhou; Bai, Yongping; Yao, Meilian; Chen, Jing; Zhang, Guogang] Cent South Univ, Xiangya Hosp, Dept Geriatr Med, Changsha, Peoples R China.;[Li, Jiayu] Hunan Univ Chinese Med, Coll Pharm, Changsha, Peoples R China.;[Zhang, Zheng] Cent South Univ, Xiangya Sch Pharmaceut Sci, Dept Pharmacol, Hunan Key Lab Cardiovasc Res, Changsha, Peoples R China.
通讯机构：
[Zhang, Zheng; Bai, Yongping] D;Department of Pharmacology, Hunan Key Laboratory of Cardiovascular Research, Xiangya School of Pharmaceutical Sciences, Central South University, Changsha, China<&wdkj&>Department of Geriatric Medicine, Xiangya Hospital, Central South University, Changsha, China<&wdkj&>National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, China
摘要：
Cancer or endothelial cells preferably catabolize glucose through aerobic glycolysis rather than oxidative phosphorylation. Intracellular ionic signaling has been shown to regulate glucose metabolism, but the underlying ion channel has yet to be identified. RNA-seq, metabolomics and genetic assay revealed that the TRPM7 channel regulated cellular glycolysis. Deletion of TRPM7 suppressed cancer cell glycolysis and reduced the xenograft tumor burden. Deficiency of endothelial TRPM7 inhibited postnatal retinal angiogenesis in mice. Mechanistically, TRPM7 transcriptionally regulated the solute carrier family 2 member 3 (SLC2A3, also known as GLUT3) via Ca2+ influx-induced calcineurin activation. Furthermore, CREB-regulated transcription coactivator 2 (CRTC2) and CREB act downstream of calcineurin to relay Ca2+ signal to SLC2A3 transcription. Expression of the constitutively active CRTC2 or CREB in TRPM7 knockout cell normalized glycolytic metabolism and cell growth. The TRPM7 channel represents a novel regulator of glycolytic reprogramming. Inhibition of the TRPM7-dependent glycolysis could be harnessed for cancer therapy.
摘要：
ETHNOPHARMACOLOGICAL RELEVANCE: Lonicerae japonicae flos (LJF) and Lonicerae flos (LF) belong to different genera of Caprifoliaceae with analogous appearances and functions. Historically, they have been used as herbal medicines to treat various diseases with confirmed wind-heat evacuation, heat-clearing, and detoxification effects. However, the Chinese Pharmacopoeia (2005 Edition) lists LJF and LF under different categories. AIM OF THE STUDY: Few studies have systematically compared the similarities and dissimilarities of LJF and LF concerning their research achievements. This systematic review and comparison of the traditional use, identification, and phytochemical and pharmacological properties of LJF and LF provides valuable insights for their further application and clinical safety. MATERIALS AND METHODS: Related document information was collected from databases that included Web of Science, X-MOL, Science Direct, PubMed, and the China National Knowledge Infrastructure. RESULTS: The chemical constituents and pharmacological effects of LJF and LF were similar. A total of 337 and 242 chemical constituents were isolated and identified in LJF and LF, respectively. These included volatile oils, cyclic ether terpenes, flavonoids, phenolic acids, triterpenoids, and their saponins. Additionally, LJF plants contain more iridoids and flavonoids than LF plants. The latter have a variety of triterpenoid saponins and significantly higher chlorogenic acid content than LJF plants. Pharmacological studies have shown that LJF and LF have various anti-inflammatory, antiviral, antibacterial, anti-endotoxic, antioxidant, anti-tumor, anti-platelet, myocardial protective, and hepatoprotective effects. CONCLUSIONS: This review was undertaken to explore whether LJF and LF should be listed separately in the Chinese Pharmacopoeia in terms of their disease prevention and treatment strategies. Although LJF and LF showed promising effects, their action mechanisms remains unclear. Specifically, their impact on gut microbiota, gastrointestinal tract, and blood parameters requires further investigation. These studies will provide the foundation for scientific utilization and clinical/non-clinical applications of LJF and LF, and the maximum benefits from their mutual use.
摘要：
Objective: The use of immune checkpoint inhibitors (ICIs) provides promising strategies for hepatocellular carcinoma (HCC) treatment. This study aimed to explore impact and underlying mechanism of the combination therapy of quercetin and anti-programmed cell death 1 (anti-PD-1) antibody on HCC. Methods: Orthotopically transplanted HCC tumors in mice were treated with quercetin, anti-PD-1 antibody, or a combination of both therapies. Histopathological changes and programmed cell death ligand 1 (PD-L1) expression were characterized by hematoxylin and eosin (H&E) and immunohistochemistry (IHC) staining. The diversity and differences of gut microbiota (GM) were evaluated through 16S rRNA sequencing. Levels of macrophage immunity-related cytokines were quantified by enzyme-linked immunosorbent assay (ELISA), quantitative real-time polymerase chain reaction (RT-qPCR), and Western blot. Results: Combination therapy reduced necrosis, fibrosis, and PD-L1 expression in liver tissues. Additionally, combination therapy reduced GM imbalance and increased abundance of Firmicutes, Actinobacteria, and Verrucomicrobiota at the phylum level as well as Dubosiella and Akkermansia at the genus level. Combination therapy improved macrophage immunity, raised the expressions of CD8a, CD4, CD11b, interleukin (IL)-10, and interferon (IFN)--y , and declined the expressions of IL-4, IL-6, toll-like receptor 4 (TLR4), an inhibitor of nuclear factor kB alpha (IkB alpha), and the NFkB subunit p65. Upon combination therapy, expressions of M2 macrophage-related genes arginase-1 (Arg-1), IL-10, transforming growth factor-beta (TGF-beta), and matrix metalloproteinase-9 (MMP-9) were upregulated. Instead, M1 macrophage-related genes IL-6, IL-12a, IL-1 beta, and tumor necrosis factor-alpha (TNF-alpha) were downregulated. Conclusions: Quercetin/anti-PD-1 antibody combination therapy reshaped HCC tumor microenvironment in mice in parallel with regulating the GM and macrophage immunity.
摘要：
BackgroundAging is an important cause of cognitive dysfunction. Liuwei Dihuang decoction (LW), a commonly applied Chinese medicine formula, is widely used for the treatment of aging-related diseases in China. Previously, LW was confirmed to be effective in prolonging life span and reducing oxidative stress in aged mice. Unfortunately, the underlying mechanism of LW remains unclear. The aim of this study was to interpret the mechanism by which LW alleviates cognitive dysfunction related to aging from the perspective of the microbiota-gut-brain axis. MethodAll C57BL/6 mice (n = 60) were randomly divided into five groups: the control, model, vitamin E (positive control group), low-dose LW and high-dose LW groups (n = 12 in each group). Except for those in the control group, D-galactose was subcutaneously injected into mice in the other groups to induce the aging model. The antiaging effect of LW was evaluated by the water maze test, electron microscopy, 16S rRNA sequencing, combined LC-MS and GC-MS metabolomics, and ELISA. ResultsLiuwei Dihuang decoction ameliorated cognitive dysfunction and hippocampal synaptic ultrastructure damage in aging mice. Moreover, LW decreased Proteobacteria abundance and increased gut microbiota diversity in aging mice. Metabolomic analysis showed that LW treatment was associated with the significantly differential abundance of 14 metabolites, which were mainly enriched in apelin signaling, sphingolipid metabolism, glycerophospholipid and other metabolic pathways. Additionally, LW affected lipid metabolism and oxidative stress in aging mice. Finally, we also found that LW-regulated microbial species such as Proteobacteria and Fibrobacterota had potential relationships with lipid metabolism, oxidative stress and hippocampal metabolites. ConclusionIn brief, LW improved cognitive function in aging mice by regulating lipid metabolism and oxidative stress through restoration of the homeostasis of the microbiota-gut-brain axis.
期刊：
Arabian Journal of Chemistry,2022年15(11):104292 ISSN：1878-5352
通讯作者：
Yu-Qing Jian<&wdkj&>Wei Wang
作者机构：
[Wang, Wei; Jiang, Sai; Peng, Cai-Yun; Yuan, Han-Wen; Jian, Yu-Qing; Zafar, Salman; Li, Bin; Xie, Qing-Ling; Wang, Meng-Yun] Hunan Univ Chinese Med, Innovat Mat Med Res Inst, Sch Pharm, TCM & Ethnomedicine Innovat & Dev Int Lab, Changsha 410208, Peoples R China.;[Wang, Wei; Chen, Wen-Ming] Hunan Univ Chinese Med, Hosp 1, Dept Pharmaceut Prod Ctr, Changsha 410208, Peoples R China.;[Liu, Bin] Hunan Univ, Coll Biol, Changsha 410082, Peoples R China.;[Zafar, Salman] Univ Peshawar, Inst Chem Sci, Peshawar 25120, Pakistan.;[Liu, Shi-Feng; Ou-Yang, Yao-Li] Hunan Kangdejia Forestry Technol Co Ltd, Yongzhou 425600, Peoples R China.
通讯机构：
[Yu-Qing Jian; Wei Wang] T;TCM and Ethnomedicine Innovation & Development International Laboratory, Innovative Material Medical Research Institute, School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China<&wdkj&>TCM and Ethnomedicine Innovation & Development International Laboratory, Innovative Material Medical Research Institute, School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China<&wdkj&>Department of Pharmaceutical Production Center, The First Hospital of Hunan University of Chinese Medicine, Changsha 410208, China
关键词：
Jinsi Huangju (JH);Chrysanthemum morifolium;Chemical constituents;Antioxidant activity;Hepatoprotective activity;Huangjusu
摘要：
Six new compounds (huangjusus A-F), including three caffeic acid glycosides (1-3), one quinic acid derivative (4), one dihydroflavone glycoside (11) and one monoterpene (31), together with thirty-eight known compounds (5-10, 12-30, 32-44), were obtained from "Jinsi Huangju" (Chrysanthemum morifolium Ramat.) flowers. Their structures were elucidated on the basis of the data obtained from different spectroscopic techniques. Among these compounds, five new (1-4 and 11) and ten known (5-9, 12, 13, 17, 40, and 42) compounds demonstrated significant 2,2'-azi no-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging effects with IC50 values of 4.22-19.90 lM. Furthermore, three new compounds (1, 11, and 31) and seven known compounds (13, 19, 21, 29, 30, 39, and 41) exhibited potent hepatoprotective activities against N-acetyl-paminophenol (APAP)-induced toxicity in HepG2 cells with the cell survival rates of 61.53 %, 63.55 %, 60.01 %, 63.05 %, 59.75 %, 59.15 %, 61.07 %, 62.72 %, 58.86 %, and 58.76 % (positive control bicyclol, 58.41 %), respectively, at a concentration of 10 lM. These results indicate the potency of the flowers against radicals and in hepatoprotections.(c) 2022 The Author(s). Published by Elsevier B.V. on behalf of King Saud University. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
作者：
Zhou, Feng;Zhong, Lu Lu;Tan, Yang;Liu, Li;Pei, Gang
期刊：
Toxicology,2022年482:153351 ISSN：0300-483X
通讯作者：
Yang Tan<&wdkj&>Li Liu<&wdkj&>Gang Pei
作者机构：
[Zhou, Feng; Pei, Gang] Hunan Univ Chinese Med, Sch Pharm, Changsha 410208, Peoples R China.;[Zhong, Lu Lu; Pei, Gang] Key Lab Modern Res TCM, Educ Dept Hunan Prov, Changsha 410208, Peoples R China.;[Zhong, Lu Lu] Hunan Univ Chinese Med, Sci & Technol Innovat Ctr, State Key Lab Breeding Base Chinese Med Powder & I, Changsha 410208, Peoples R China.;[Tan, Yang] Hunan Prov Key Lab TCM Prevent & Treatment Depress, Changsha 410208, Peoples R China.;[Liu, Li] Second Peoples Hosp Hunan Prov, Dept Pharm, Changsha 410021, Peoples R China.
通讯机构：
[Yang Tan] H;[Li Liu] D;[Gang Pei] S;Hunan Provincial Key Laboratory of TCM Prevention and Treatment of Depression Diseases, Changsha 410208, China<&wdkj&>Department of Pharmacy, Second People's Hospital of Hunan Province, Changsha 410021, China<&wdkj&>School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China<&wdkj&>Key Laboratory of Modern Research of TCM, Education Department of Hunan Province, Changsha 410208, China
摘要：
Triptolide (TP) is the major active ingredient of Tripterygium wilfordii Hook, a traditional Chinese herb that possesses various pharmacological activities and has been used to treat autoimmune and inflammatory diseases for thousands of years. However, the clinical application of TP is limited due to its multiorgan toxicity, and in particular, its negative impact on female fertility. To date, the specific toxic mechanisms on reproduction induced by TP remain unclear. In the current study, an LC-MS/MS-based metabolomic approach was adopted to study TP-induced reproductive toxicity and its mechanism. Histopathological examination of the ovaries showed that TP significantly induced follicular atresia and decreased the numbers of corpus luteum in rats, as well as reducing the gonadal index and destroying the microstructure of the ovary. Immunohistochemical staining revealed that TP significantly induced apoptosis of rat follicle cells. Metabolomics analysis revealed that 67 and 74 small molecule metabolites in the ovaries and serum, respectively (fold-changes > 1.5, p < 0.05), were significantly different in TP-treated rats compared to CON group rats. Target profiling identified the metabolites arachidonic acid, prostaglandin D2, prostaglandin H2 and prostaglandin E2 as potential serum biomarkers for TP-induced ovary damage.
期刊：
Food Science and Technology,2022年42 ISSN：1475-3324
通讯作者：
Zhang, X.
作者机构：
[Qi, Zhechen; Gong, Majie; Nie, Xianxian; Zhang, Xiaodan; Yang, Dongfeng; Chen, Yue] Zhejiang Sci Tech Univ, Coll Lift Sci & Med, Key Lab Plant Secondary Metab & Regulat, Hangzhou, Zhejiang, Peoples R China.;[Jin, Qinghao; Gong, Majie] Zhejiang Yang Sheng Tang CO LTD, Nat Med Inst, Hangzhou, Peoples R China.;[Liu, Xiangqian] Hunan Univ Chinese Med, Sch Pharm, Changsha, Peoples R China.
通讯机构：
Key Laboratory of Plant Secondary Metabolism and Regulation, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Xiasha Higher Education Zone, Zhejiang, Hangzhou, China
关键词：
purple Eleutherococcus;botanical identification;extraction optimazation;determination of anthocyanins
期刊：
Computers in Biology and Medicine,2022年151(Pt A):106298 ISSN：0010-4825
通讯作者：
Zhang, Bikui;Li, Wenqun
作者机构：
[Liu, Sa; Liu, Jiaqin; Liu, Jian; Li, Wenqun; Zhang, Bikui] Cent South Univ, Xiangya Hosp 2, Dept Pharm, Changsha 410011, Hunan, Peoples R China.;[Liu, Sa; Liu, Jiaqin; Liu, Jian; Li, Wenqun; Zhang, Bikui] Cent South Univ, Inst Clin Pharm, Changsha 410011, Hunan, Peoples R China.;[Sun, Taoli] Hunan Univ Chinese Med, Sch Pharm, Changsha 410208, Hunan, Peoples R China.;[Fang, Senbiao] Cent South Univ, Sch Comp Sci & Engn, Hunan Prov Key Lab Bioinformat, Changsha 410083, Peoples R China.;[Tan, Shengyu] Cent South Univ, Xiangya Hosp 2, Dept Gerontol, Changsha 410011, Hunan, Peoples R China.
通讯机构：
[Bikui Zhang; Wenqun Li] D;Department of Pharmacy, The Second Xiangya Hospital, Central South University, Changsha, Hunan, 410011, China<&wdkj&>Institute of Clinical Pharmacy, Central South University, Changsha, Hunan, 410011, China<&wdkj&>Department of Pharmacy, The Second Xiangya Hospital, Central South University, Changsha, Hunan, 410011, China<&wdkj&>Institute of Clinical Pharmacy, Central South University, Changsha, Hunan, 410011, China
关键词：
Cepharanthine;COVID-19;ACE2;PI3K-Akt signal pathway;Network pharmacology;RNA-Sequencing;Molecular docking
摘要：
OBJECTIVES: Recently, it has been reported that cepharanthine (CEP) is highly likely to be an agent against Coronavirus disease 2019 (COVID-19). In the present study, a network pharmacology-based approach combined with RNA-sequencing (RNA-seq), molecular docking, and molecular dynamics (MD) simulation was performed to determine hub targets and potential pharmacological mechanism of CEP against COVID-19. METHODS: Targets of CEP were retrieved from public databases. COVID-19-related targets were acquired from databases and RNA-seq datasets GSE157103 and GSE155249. The potential targets of CEP and COVID-19 were then validated by GSE158050. Hub targets and signaling pathways were acquired through bioinformatics analysis, including protein-protein interaction (PPI) network analysis and enrichment analysis. Subsequently, molecular docking was carried out to predict the combination of CEP with hub targets. Lastly, MD simulation was conducted to further verify the findings. RESULTS: A total of 700 proteins were identified as CEP-COVID-19-related targets. After the validation by GSE158050, 97 validated targets were retained. Enrichment results indicated that CEP acts on COVID-19 through multiple pathways, multiple targets, and overall cooperation. Specifically, PI3K-Akt signaling pathway is the most important pathway. Based on PPI network analysis, 9 central hub genes were obtained (ACE2, STAT1, SRC, PIK3R1, HIF1A, ESR1, ERBB2, CDC42, and BCL2L1). Molecular docking suggested that the combination between CEP and 9 central hub genes is extremely strong. Noteworthy, ACE2, considered the most important gene in CEP against COVID-19, binds to CEP most stably, which was further validated by MD simulation. CONCLUSION: Our study comprehensively illustrated the potential targets and underlying molecular mechanism of CEP against COVID-19, which further provided the theoretical basis for exploring the potential protective mechanism of CEP against COVID-19.
摘要：
The gut microbiota plays an important role in central nervous system (CNS) disorders. Apolipoprotein E (ApoE) can affect the composition of the gut microbiota and is closely related to the CNS. However, the mechanism by which ApoE affects cognitive dysfunction through the gut microbiota-brain axis has thus far not been investigated. In this study, we used wild-type mice and ApoE knockout (ApoE(-/-)) mice to replicate the aging model and examined the effects of ApoE deletion on cognitive function, hippocampal ultrastructure, synaptophysin (SYP) and postsynaptic density 95 (PSD-95) in aging mice. We also explored whether ApoE deletion affects the gut microbiota and the metabolite profile of the hippocampus in aging mice and finally examined the effect of ApoE deletion on lipids and oxidative stress in aging mice. The results showed that the deletion of ApoE aggravated cognitive dysfunction, hippocampal synaptic ultrastructural damage and dysregulation of SYP and PSD-95 expression in aging mice. Furthermore, ApoE deletion reduced gut microbial makeup in aging mice. Further studies showed that ApoE deletion altered the hippocampal metabolic profile and aggravated dyslipidemia and oxidative stress in aging mice. In brief, our findings suggest that loss of ApoE alters the composition of the gut microbiota, which in turn may affect cognitive function in aging mice through the gut microbiota-brain axis.
作者机构：
[Cai, Xiong; Qin, Yan; Cai, X; Shehla, Nuzhat; Peng, Caiyun; Wang, Wei; Cao, Liang; Li, Bin; Yu, Huanghe; Qiu, Yixing; Lin, Ye; Daniyal, Muhammad] Hunan Univ Chinese Med, Sch Pharm, Innovat Mat Med Res Inst, TCM & Ethnomed Innovat & Dev Int Lab, Changsha 410208, Peoples R China.;[Liu, B; Fan, Jialong; Wang, Zhou; Liu, Bin] Hunan Univ, Coll Biol, Changsha 410082, Hunan, Peoples R China.
通讯机构：
[Liu, B ; Cai, X; Wang, W ] H;Hunan Univ Chinese Med, Sch Pharm, Innovat Mat Med Res Inst, TCM & Ethnomed Innovat & Dev Int Lab, Changsha 410208, Peoples R China.;Hunan Univ, Coll Biol, Changsha 410082, Hunan, Peoples R China.
关键词：
rheumatoid arthritis;xuetongsu;biomimetic hybrid membrane;targeted delivery;photothermal therapy;prussian blue nanoparticles
期刊：
Frontiers in Veterinary Science,2022年9:1434 ISSN：2297-1769
作者机构：
[Wang, Aibing; Tan, Lei; Wang, Naidong; Lei, Hongyu; Yang, Lincheng; Lei, Lei; Duan, Deyong; Yang, Yi] Hunan Agr Univ HUNAU, Coll Vet Med, Hunan Prov Key Lab Prot Engn Anim Vaccines, Lab Anim Dis Prevent & Control & Anim Model, Changsha, Peoples R China.;[Zhou, Yujun] Hunan Sino Sci Gene Technol Co Ltd, Changsha, Peoples R China.;[Wang, Wei; Qiu, Yixing] Hunan Univ Chinese Med, Academician Atta ur Rahman Belt & Rd Tradit Med Re, Sch Pharm, TCM & Ethnomedicine Innovat & Dev Int Lab, Changsha, Peoples R China.;[Wang, Cheng] Xiangxi Prefecture Anim Husb & Aquat Prod Affairs, Xiangxi, Peoples R China.;[Yao, Jun; Zhu, Pei] Yunnan Anim Sci & Vet Inst, Yunnan Trop & Subtrop Anim Virus Dis Lab, Kunming, Peoples R China.
关键词：
pseudorabies virus;Porcine industry related practitioners;Questionnaire survey;China;Epidemiology
摘要：
Pseudorabies virus (PRV) is widely prevalent in China, which can transmit from pigs to other mammals. Moreover, a PRV variant isolated from an acute human encephalitis case was documented recently. It is imperative to investigate PRV epidemiology in pigs, the knowledge regarding pseudorabies (PR) and self-protection behaviors upon working among relevant practitioners including pig farmers, pig cutters, and pork salesman. In the present study, 18,812 pig serum samples and 1,634 tissue samples were collected from Hunan Province during the period of 2020 to 2021 for detecting the presence of PRV gE-special antibody and nucleic acids, respectively. Meanwhile, we conducted a questionnaire survey about PR among these practitioners in China. The results showed that nearly 9% (1,840/20,192) pigs from 161 collected sites (20.17%, 161/797) were seropositive for PRV-gE antibody. Though only 2.33% tissue samples were positive for PRV nucleic acids, all the representative PRV strains were variant. It was learned that most practitioners were frequently injured when working, the injured sites mainly included hand and foot. Among the three transmission routes of PRV, the aerosol transmission route was often overlooked. Moreover, the workers lacked self-protection awareness and were poor conscious about PRV and its potential threat to humans. All the results demonstrate that PRV remains widely spread in pig populations, while the potential threats of PRV in pig industry receive less attention, suggesting that targeted educational programs to these people should be performed.
期刊：
Journal of Applied Toxicology,2022年43(6):772-788 ISSN：0260-437X
通讯作者：
Gang Pei
作者机构：
[Zhou, Feng; Yang, Xuebin; Tan, Yang; Zhong, Lulu; Pei, Gang] Hunan Univ Chinese Med, Pharm Coll, Changsha, Peoples R China.;[Zhou, Feng; Yang, Xuebin; Tan, Yang; Zhong, Lulu; Pei, Gang] Educ Dept Hunan Prov, Key Lab Modern Res TCM, Changsha, Peoples R China.;[Luo, Yue] Liuyang Peoples Hosp, Dept Pharm, Changsha, Peoples R China.;[Li, Shunmin] Shenzhen Tradit Chinese Med Hosp, Shenzhen, Peoples R China.
通讯机构：
[Gang Pei] P;Pharmacy of College, Hunan University of Chinese Medicine, Changsha, China<&wdkj&>Key Laboratory of Modern Research of TCM, Education Department of Hunan Province, Changsha, China
关键词：
apoptosis;female reproductive toxicity;natural products;ovarian granulosa cell;traditional Chinese medicine
摘要：
Abnormal ovarian function is the main manifestation of female reproductive toxicity. Granulosa cells (GCs) play an important role in determining the fate of follicles and are the main effector cells of the female reproductive system. Excessive apoptosis of GCs leads to pathological folliculogenesis and further reproductive damage. However, drugs available for treatment of female reproductive toxicity are limited. Recent studies have confirmed that various natural products and bioactive ingredients of traditional Chinese medicine (TCM) can inhibit apoptosis of GCs and protect ovarian function. In this review, the mechanisms underlying the proapoptotic and antiapoptotic effects of natural products and bioactive ingredients of TCM on the proliferation, function, and apoptosis of GCs are summarized based on the findings of reports published over the past 10 years as reference for the treatment of female reproductive toxicity.
作者机构：
[Chen, Chen; Peng, Ye; Chen, Nai-hong; Jian, Wen-xuan; Chu, Shi-feng; Liu, Dan-dan; Zhang, Zhao; Zhu, Jie] Chinese Acad Med Sci & Peking Union Med Coll, State Key Lab Bioact Subst & Funct Nat Med, Inst Mat Med, Beijing 100050, Peoples R China.;[Chen, Chen; Peng, Ye; Chen, Nai-hong; Jian, Wen-xuan; Chu, Shi-feng; Liu, Dan-dan; Zhang, Zhao; Zhu, Jie] Chinese Acad Med Sci & Peking Union Med Coll, Neurosci Ctr, Beijing 100050, Peoples R China.;[Peng, Ye; Chen, Nai-hong] Hunan Univ Chinese Med, Coll Pharm, Changsha 410208, Peoples R China.;[Chen, Nai-hong; Jian, Wen-xuan] Guangzhou Univ Chinese Med, Inst Clin Pharmacol, Guangzhou 510720, Peoples R China.;[Sun, Hong-shuo; Feng, Zhong-ping] Univ Toronto, Fac Med, Dept Physiol, Toronto, ON, Canada.
通讯机构：
[Zhang, Zhao; Chen, Nai-Hong] S;State Key Laboratory of Bioactive Substances and Functions of Natural Medicines, Institute of Materia Medica & Neuroscience Center, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100050, China.;College of Pharmacy, Hunan University of Chinese Medicine, Changsha, 410208, China.;Institute of Clinical Pharmacology, Guangzhou University of Chinese Medicine, Guangzhou, 510720, China.
