作者：
Zhou, Feng;Zhang, Guo Dong;Tan, Yang;Hu, Shi An;Tang, Qun*;...
期刊：
Therapeutic Advances in Gastroenterology,2023年16:17562848231176889 ISSN：1756-283X
通讯作者：
Tang, Qun;Pei, G;Tang, Q
作者机构：
[Tang, Qun; Zhou, Feng; Tang, Q; Pei, G; Pei, Gang] Hunan Univ Chinese Med, Sch Pharm, Changsha 410208, Peoples R China.;[Tang, Qun; Tang, Q] Hunan Univ Chinese Med, Med Sch, Changsha, Peoples R China.;[Zhou, Feng; Pei, G; Pei, Gang] Educ Dept Hunan Prov, Key Lab Modern Res TCM, Changsha, Peoples R China.;[Zhang, Guo Dong] Ningxia Med Univ, Yinchuan, Ningxia, Peoples R China.;[Tan, Yang; Hu, Shi An] Hunan Univ Chinese Med, Sch Pharm, Changsha, Peoples R China.
通讯机构：
[Tang, Q; Pei, G ; Tang, Q ] H;Hunan Univ Chinese Med, Sch Pharm, Changsha 410208, Peoples R China.;Hunan Univ Chinese Med, Med Sch, Changsha, Peoples R China.;Educ Dept Hunan Prov, Key Lab Modern Res TCM, Changsha, Peoples R China.
摘要：
Therapeutic Advances in Gastroenterology, Volume 16, Issue , January-December 2023. <br/>Inflammatory bowel disease (IBD) is a chronic gastrointestinal inflammatory disease that involves host genetics, the microbiome, and inflammatory responses. The current consensus is that the disruption of the intestinal mucosal barrier is the core pathogenesis of IBD, including intestinal microbial factors, abnormal immune responses, and impaired intestinal mucosal barrier. Cumulative data show that nucleotide-binding and oligomerization domain (NOD)-like receptors (NLRs) are dominant mediators in maintaining the homeostasis of the intestinal mucosal barrier, which play critical roles in sensing the commensal microbiota, maintaining homeostasis, and regulating intestinal inflammation. Blocking NLRs inflammasome activation by botanicals may be a promising way to prevent IBD progression. In this review, we systematically introduce the multiple roles of NLRs in regulating intestinal mucosal barrier homeostasis and focus on summarizing the activities and potential mechanisms of natural products against IBD. Aiming to propose new directions on the pathogenesis and precise treatment of IBD
摘要：
OBJECTIVES: The clinical features of aseptic meningitis associated with amoxicillin are unknown. The main objective of this study was to investigate the clinical characteristics of amoxicillin-induced aseptic meningitis (AIAM) and provide a reference for clinical diagnosis and treatment. METHODS: AIAM-related studies were collected by searching the relevant databases from inception to October 31, 2022. RESULTS: AIAM usually occurred 3h to 7days after amoxicillin administration in 13 males and 9 females. Twenty-one patients (95.5%) had recurrent AIAM with a total of 62 episodes. Fever (19 cases, 86.4%) and headache (18 cases, 81.8%) were the most common symptoms. Typical cerebrospinal fluid (CSF) findings were leukocytosis (100%) with lymphocytic predominance (14 cases, 63.6%), elevated protein (20 cases, 90.1%), normal glucose (21 cases, 95.5%) and negative culture (21 cases, 100%). Brain magnetic resonance imaging showed mild meningeal enhancement in one patient. The symptoms resolved mainly within 1-4days after drug discontinuation in all patients. CONCLUSION: Clinical attention should be given to the adverse effects of AIAM. The medication history of patients with suspected meningitis should be investigated to avoid unnecessary examination and antibiotic treatment.
摘要：
Saponins extracted from Panax notoginseng leaves by methanol or water could be orally administrated for insomnia with very low bioavailability, which might be bio-converted by gut microbiota to generate potential bioactive products. Moreover, gut microbiota profiles from insomniac patients are very different from healthy subjects. We aimed to compare the metabolic characteristics and profiles of the two saponins extract by incubation with gut microbiota from insomniac patients. The ginsenosides, notoginsenosides and metabolites were identified and relatively quantified by HPLC-MS/MS. Gut microbiota were profiled by 16S ribosomal RNA gene sequencing. The results showed that saponins were very different between methanol or water extract groups, which were metabolized by gut microbiota to generate the similar yields. The main metabolites included ginsenoside Rd, ginsenoside F(2) , ginsenoside C-Mc or ginsenoside C-Y, ginsenoside C-Mx, ginsenoside compound K, and protopanaxadiol in both groups, while gypenoside XVII, notoginsenoside Fe, ginsenoside Rd(2) , and notoginsenoside Fd were the intermediates in the methanol group. Moreover, the microbial, Faecalibacterium prausnitzi, could bio-convert the saponins to obtain the corresponding metabolites. Our study implied that saponins extracted from P. notoginseng leaves by methanol or water could be used for insomniac patients due to gut microbiota biotransformation. This article is protected by copyright. All rights reserved.
期刊：
Frontiers in Pharmacology,2023年13 ISSN：1663-9812
作者机构：
[Duan, Yan; Li, Shun-Xiang; Tang, Min; Li, Juan; Su, Yu-Ting; Zhou, Qun] Hunan Univ Chinese Med, Hunan Engn Technol Res Ctr Bioact Subst Discovery, Sch Pharm, Changsha, Peoples R China.;[Duan, Yan; Li, Shun-Xiang; Ren, Jie; Tang, Min; Li, Juan; Su, Yu-Ting; Zhou, Qun] Hunan Prov Sino US Int Joint Res Ctr Therapeut Dru, Changsha, Peoples R China.
关键词：
osteoporosis;kidney tonifying traditional Chinese medicine;pharmacological mechanism;active ingredients;safety and toxicity
摘要：
The aging global population is increasingly affected by osteoporosis (OP), which is one of the most significant threats to the elderly. Moreover, its prevention and treatment situations have become increasingly severe. Therefore, it is imperative to develop alternatives or complementary drugs for preventing and treating osteoporosis. Kidney tonifying traditional Chinese medicine (KTTCM) has been used for the treatment of osteoporosis for a long time. Pharmacological studies have shown that kidney tonifying traditional Chinese medicine can promote osteoblasts, inhibit osteoclasts, and regulate the level of estrogen and plays vital roles in stimulating osteogenesis, restraining adipogenesis of marrow mesenchymal stem cells (MSCs), regulating the metabolism of calcium and phosphorus, and inhibiting oxidative stress. These effects are mediated by OPG/RANKL/RANK, BMP/Smads, MAPKs, and Wnt/beta-catenin systems. To develop a safe, synergistic, effective, and homogenized TCM formula with robust scientific evidence to provide faster and more economical alternatives, the anti-osteoporosis ingredients and pharmacological mechanisms of kidney tonifying traditional Chinese medicine are recapitulated from the perspective of molecular and cell biology, and the safety and toxicity of kidney tonifying traditional Chinese medicine have also been reviewed in this paper.
作者机构：
[Liu, Xiangqian; Wei, Chunling; Cui, Peiwu] Hunan Univ Chinese Med, Coll Pharm, Changsha 410208, Peoples R China.;[Wei, Chunling; Cui, Peiwu] Natl Adm TCM, Res Lab TCM Property & Efficacy, Level 3, Changsha 410208, Peoples R China.;[Wei, Chunling; Cui, Peiwu] Hunan Univ Chinese Med, Mycomedicine Res Lab, Changsha 410208, Peoples R China.
通讯机构：
[Peiwu Cui; Xiangqian Liu] C;College of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China<&wdkj&>Research Lab of TCM Property & Efficacy, Level 3, National Administration of TCM, Changsha 410208, China<&wdkj&>Mycomedicine Research Lab, Hunan University of Chinese Medicine, Changsha 410208, China<&wdkj&>College of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China
摘要：
Antibacterial resistance has become one of the most serious problems threating global health. To overcome this urgent problem, many scientists have paid great attention to developing new antibacterial drugs from natural products. Hence, for exploring new antibacterial drugs from Chinese medicine, a series of experiments were carried out for verifying and elucidating the antibacterial activity and mechanisms of madecassic acid (MA), which is an active triterpenoid compound isolated from the traditional Chinese medicine, Centella asiatica. The antibacterial activity was investigated through measuring the diameter of the inhibition zone, the minimum inhibitory concentration (MIC), the growth curve, and the effect on the bacterial biofilm, respectively. Meanwhile, the antibacterial mechanism was also discussed from the aspects of cell wall integrity variation, cell membrane permeability, and the activities of related enzymes in the respiratory metabolic pathway before and after the intervention by MA. The results showed that MA had an inhibitory effect on eight kinds of pathogenic bacteria, and the MIC values for Staphylococcus aureus, Methicillin-resistant Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, and Bacillus megaterium were 31.25, 62.5, 250, 125, 62.5, and 62.5 mu g/mL, respectively. For instance, 31.25 mu g/mL MA could inhibit the growth of Staphylococcus aureus within 28 h. The antibacterial mechanism experiments confirmed that MA could destroy the integrity of the cell membrane and cell wall of Staphylococcus aureus, causing the leakage of macromolecular substances, inhibiting the synthesis of soluble proteins, reducing the activities of succinate dehydrogenase and malate dehydrogenase, and interacting with DNA, leading to the relaxation and ring opening of supercoiled DNA. Besides, the activities of DNA topoisomerase I and II were both inhibited by MA, which led to the cell growth of Staphylococcus aureus being repressed. This study provides a theoretical basis and reference for the application of MA in the control and inhibition of food-borne Staphylococcus aureus.
作者机构：
[Sun, Wei; Wang, Chunjiang; Li, Zuojun; Wu, Tian] Cent South Univ, Xiangya Hosp 3, Dept Pharm, Changsha 410013, Hunan, Peoples R China.;[Fan, Zhiqiang; He, Yang] Hunan Univ Chinese Med, Hosp 1, Dept Pharm, Changsha 410007, Hunan, Peoples R China.
摘要：
Background. Acute generalized exanthematous pustulosis (AGEP) is a rare and serious adverse reaction of terbinafine. Understanding AGEP and terbinafine is primarily based on case reports. The purpose is to explore the clinical characteristics of terbinafine-induced AGEP, and to provide reference for clinical diagnosis and treatment. Methods. Case reports and original studies of terbinafine-induced AGEP were retrieved by searching Chinese and English databases from January, 1991, to May 31, 2022. Results. The median age of the 32 patients (17 males and 15 females) was 55 years (range: 6-84). The median time to onset of AGEP is 8 days (range: 1-77) and is usually accompanied by fever (>38 degrees C) and elevated neutrophil levels (>8000/mm(3)). Four patients (12.5%) had oral mucosal involvement, and 10 patients (31.3%) developed postpustular desquamation. The lesions were mainly distributed in the trunk (43.8%), the whole body (34.4%), and the extremities (53.1%). Skin biopsy revealed subcorneal pustules (65.6%), intraepidermal cavernous pustules (43.8%), necrotic keratinocytes (15.6%), spongy hyperplasia (25.0%), neutrophil exocytosis (34.4%), and papillary dermal edema (40.6%). AGEP resolved completely in all patients at a median time of 12 days (range: 2-90) after discontinuation of terbinafine and symptomatic therapy. Conclusion. Clinicians should be aware that terbinafine-induced AGEP is a rare cutaneous adverse reaction. AGEP usually occurs within 2 weeks after administration and has a good prognosis after discontinuation.
作者机构：
[Tang, Yan-Ran; Choudhary, M. Iqbal; Wang, Wei; Ali, Sajjad; Peng, Cai-Yun; Yi, Pan; Peng, CY; Zhou, Fang; Rahman, Atta-ur; Zafar, Salman; Li, Bin; Sheng, Wen-Bing; Mao, Yu] Hunan Univ Chinese Med, Acad Attaur Rahman Belt & Rd Tradit Med Res Ctr, Sch Pharm, ATCM & Ethnomedicine Innovat Dev Int Lab, Changsha, Peoples R China.;[Tang, Yan-Ran; Yi, Pan] Hunan Food & Drug Vocat Coll, Coll Tradit Chinese Med, Changsha, Peoples R China.;[Zafar, Salman] Univ Peshawar, Inst Chem Sci, Peshawar, Pakistan.;[Ali, Sajjad] Karakoram Int Univ, Dept Chem, Gilgit, Pakistan.;[Zhou, Fang; Chen, Wen-Ming] Hunan Univ Chinese Med, Hosp 1, Changsha, Peoples R China.
通讯机构：
[Peng, CY; Wang, W] H;Hunan Univ Chinese Med, Acad Attaur Rahman Belt & Rd Tradit Med Res Ctr, Sch Pharm, ATCM & Ethnomedicine Innovat Dev Int Lab, Changsha, Peoples R China.
摘要：
Three new constituents: 1,5R-dihydroxy-3,8S-dimethoxy-5,6,7,8-tetrahydroxanthone (1), (3S,4R,16S,17R)-3,16,23-trihydroxyoleana-11,13(18)-dien-28-aldehyde-3-O-beta-D-glucopyranoside (2), and new natural product (S)-gentiandiol (3), along with 41 known compounds were isolated from Tujia ethnomedicine Shuihuanglian, namely, the whole plant of Swertia punicea. Structures of all these compounds were established through extensive spectroscopic techniques, namely 1D, 2D-NMR spectroscopy, HRESIMS analysis, and the absolute configuration of the new compounds was discerned by circular dichroism (CD) spectroscopy. Antioxidative effects of these compounds were evaluated by using the DPPH radical scavenging method, compounds 7, 9 and 14 showed antioxidant activities with IC50 values of 68.9, 50.8 and 48.2 mu M, respectively. [GRAPHICS]
通讯机构：
[Tan, Y ; Pei, G] H;Hunan Univ Chinese Med, Pharm Coll, Changsha 410208, Peoples R China.;Educ Dept Hunan Prov, Key Lab Modern Res TCM, Changsha, Peoples R China.
摘要：
OBJECTIVES: Shaoyao Gancao Decoction (SGD) is a well-known Chinese herbal prescription used to treat ulcerative colitis (UC). This study was designed to evaluate the effect of SGD in dextran sulfate sodium-induced UC and to reveal the potential mechanism. METHODS: A UC mouse model was established by the administration of dextran sulfate sodium. The mice were given SGD extract intragastrically for 7 days. Histological pathology, inflammatory factors, and ferroptosis regulators were determined in vivo. In addition, ferroptotic Caco-2 cells were prepared to investigate the underlying mechanism of the effects of SGD. KEY FINDINGS: The results showed that SGD reduced the disease activity index, the level of inflammatory factors, and histological damage in mice with UC. Moreover, SGD down-regulated the level of ferroptosis in cells in colon tissue, as evidenced by a reduced iron overload, decreased glutathione depletion, and a lower level of malondialdehyde production, compared with the model group. Correspondingly, similar effects of SGD on ferroptosis were observed in Erastin-treated Caco-2 cells. The results of our in vitro reactive oxygen species assays and the changes in mitochondrial structure observed by scanning electron microscopy also supported these results. CONCLUSION: Taken together, these findings suggest that SGD protected against UC by down-regulating ferroptosis in colonic tissue.
期刊：
Journal of Materials Chemistry B,2023年11(10):2219-2233 ISSN：2050-750X
通讯作者：
Liqin Yuan<&wdkj&>Bin Liu
作者机构：
[Liu, Bin; Fan, Jialong; Qin, Yan; Xiao, Feng; Long, Ying; Chen, Simin] Hunan Univ, Coll Biol, Changsha 410082, Peoples R China.;[Yuan, Liqin] Cent South Univ, Xiangya Hosp 2, Dept Gen Surg, Changsha 410011, Hunan, Peoples R China.;[Qin, Yan] Hunan Univ Chinese Med, Sch Pharm, TCM & Ethnomed Innovat & Dev Int Lab, Changsha 410208, Hunan, Peoples R China.
通讯机构：
[Liqin Yuan] D;[Bin Liu] C;College of Biology, Hunan University, Changsha, P. R. China<&wdkj&>Department of General Surgery, The Second Xiangya Hospital, Central South University, Changsha, Hunan, P. R. China
摘要：
Although combined photodynamic/photothermal therapy (PDT/PTT) has been used for cancer theranostics recently, their therapeutic efficacy has been compromised by the low O(2) partial pressure and high concentration of GSH in the tumor microenvironment (TME). Thus, the construction of intelligent TME-responsive nanocomplexes is a powerful strategy for addressing the above issues. In this study, MnO(2)-coated Prussian blue nanocomplexes (PM NPs) were designed as O(2) suppliers and GSH depletion agents to reprogram the TME. Subsequently, tumor-targeting peptide (RGD)-modified erythrocyte membrane vesicles loaded with photosensitizer (Ce6) were used to camouflage PM NPs (PMRCR NPs). Importantly, the prepared PMRCR NPs exhibited excellent photothermal performance with a photothermal conversion efficiency of 44.9%. Moreover, the in vitro PDT/PTT was enhanced, by which the cell viability was reduced to 21.4%, which is lower than the 55.6% (PDT) and 66.7% (PTT) of PMRCR NPs with a single laser treatment. By modeling 4T1 tumor-bearing mice, the combined PDT/PTT of PMRCR NPs greatly inhibited tumor growth, and after 20 days, a tumor inhibition rate of 92.9% was achieved. This work provides a promising strategy by developing TME-reprogrammed multifunctional nanocomplexes to enhance PDT/PTT antitumor efficacy.
期刊：
Journal of Agricultural and Food Chemistry,2023年71(18):6999-7008 ISSN：0021-8561
作者机构：
[Yu, Yigang; Li, Xiaoqing] South China Univ Technol, Coll Food Sci & Engn, Guangzhou 510006, Guangdong, Peoples R China.;[Peng, Xinan; Li, Xiaoqing; Cao, Yong; Hu, Hao; Lin, Qianru; Jiang, Yiqi; Ye, Zhuming; Liu, Guo] South China Agr Univ, Coll Food Sci, Guangdong Prov Key Lab Nutraceut & Funct Foods, Guangzhou 510642, Guangdong, Peoples R China.;[Ouyang, Wen] Hunan Univ Chinese Med, Sch Pharm, Changsha 410208, Hunan, Peoples R China.;[Ouyang, Wen] Liuyang Hosp Tradit Chinese Med, Liuyang 410300, Hunan, Peoples R China.
关键词：
DSS;Phyllanthus emblica L. extract;colonic inflammation;gut microbiome;transcriptome analysis
摘要：
The anti-inflammation effect of aqueous Phyllanthus emblica L. extract (APE) and its possible underlying mechanism in dextran sulfate sodium (DSS)-induced mice chronic colonic inflammation were studied. APE treatment significantly improved the colitic symptoms, including ameliorating the shortening of the colon, increasing the DSS-induced body weight loss, reducing the disease activity index, and reversing the condition of colon tissue damage of mucus lost and goblet cell reduction. Overproduction of serum pro-inflammatory cytokines were suppressed by the treatment of APE. Gut microbiome analysis showed that APE remodeled the structure of gut bacteria in phylum and genus levels, upregulating the abundance of phylum Bacteroidetes, family Muribaculaceae, and genus Bacteroides and downregulating the abundance of phylum Firmicutes. The reshaped gut microbiome caused metabolic functions and pathway change with enhanced queuosine biosynthesis and reduced polyamine synthesis pathway. Colon tissue transcriptome analysis further elucidated APE-inhibited mitogen-activated protein kinase (MAPK), cytokine-cytokine receptor interaction, and tumor necrosis factor (TNF) signaling pathways and the expressions of the genes that promote the progress of colorectal cancer. It turned out that APE reshaped the gut microbiome and inhibited MAPK, cytokine-cytokine receptor interaction, and TNF signaling pathways as well as the colorectal-cancer-related genes to exert its colitis protective effect.
期刊：
Frontiers in Chemistry,2023年11:256 ISSN：2296-2646
作者机构：
[Li, Jiani; Zhou, Yimiao; Xiao, Zuowei; Deng, Bin; Li, Xiang] Hunan Univ Chinese Med, Coll Pharm, Changsha, Peoples R China.;[Li, Jiani; Xiao, Zuowei; Deng, Bin; Li, Xiang] Hunan Univ Chinese Med, Key Disciplines Pharm, Changsha, Hunan, Peoples R China.;[Li, Jiani; Xiao, Zuowei; Deng, Bin; Li, Xiang] Hunan Univ Chinese Med, Changsha, Hunan, Peoples R China.;[Li, Jiani; Xiao, Zuowei; Deng, Bin; Li, Xiang] Food Homol Innovat Lab, Changsha, Hunan, Peoples R China.;[Zhou, Yimiao] Hunan Univ Chinese Med, Xiangxing Coll, Xiangyin Campus, Changsha, Peoples R China.
关键词：
Potassium ion battery;Biomass carbon;anode materials;Doping;storage capacity
摘要：
Biochar materials have attracted people’s attention because of their environmental friendliness, abundant resources, and the use of waste resources for reuse. As a potassium-ion anode material, biomass char materials synthesized by different methods have broad application prospects. However, due to the problems of low initial magnification and limited potassium-storage capacity, it is necessary to improve the electrochemical performance through modifications, such as atomic doping. Atomic doping is an effective way to improve battery conductivity and potassium storage. In this paper, the synthesis method of biochar as an anode material for potassium-ion batteries and the influence of atomic doping on its modification in recent years are reviewed.
摘要：
BackgroundForkhead box M1 (FOXM1) is a member of the Forkhead box (Fox) transcription factor family. It regulates cell mitosis, cell proliferation, and genome stability. However, the relationship between the expression of FOXM1 and the levels of m6a modification, immune infiltration, glycolysis, and ketone body metabolism in HCC has yet to be fully elucidated. MethodsTranscriptome and somatic mutation profiles of HCC were downloaded from the TCGA database. Somatic mutations were analyzed by maftools R package and visualized in oncoplots. GO, KEGG and GSEA function enrichment was performed on FOXM1 co-expression using R. We used Cox regression and machine learning algorithms (CIBERSORT, LASSO, random forest, and SVM-RFE) to study the prognostic value of FOXM1 and immune infiltrating characteristic immune cells in HCC. The relationship between FOXM1 and m6A modification, glycolysis, and ketone body metabolism were analyzed by RNA-seq and CHIP-seq. The competing endogenous RNA (ceRNA) network construction relies on the multiMiR R package, ENCORI, and miRNET platforms. ResultsFOXM1 is highly expressed in HCC and is associated with a poorer prognosis. At the same time, the expression level of FOXM1 is significantly related to the T, N, and stage. Subsequently, based on the machine learning strategies, we found that the infiltration level of T follicular helper cells (Tfh) was a risk factor affecting the prognosis of HCC patients. The high infiltration of Tfh was significantly related to the poor overall survival rate of HCC. Besides, the CHIP-seq demonstrated that FOXM1 regulates m6a modification by binding to the promoter of IGF2BP3 and affects the glycolytic process by initiating the transcription of HK2 and PKM in HCC. A ceRNA network was successfully obtained, including FOXM1 - has-miR-125-5p - DANCR/MIR4435-2HG ceRNA network related to the prognosis of HCC. ConclusionOur study implicates that the aberrant infiltration of Tfh associated with FOXM1 is a crucial prognostic factor for HCC patients. FOXM1 regulates genes related to m6a modification and glycolysis at the transcriptional level. Furthermore, the specific ceRNA network can be used as a potential therapeutic target for HCC.
期刊：
Pain Research and Management,2023年2023:7711988 ISSN：1203-6765
通讯作者：
Ren, WQ;Long, HP
作者机构：
[Chen, Lei; Zhao, Hongxia; Zeng, Shuiqing; Ren, Weiqiong; Liu, Yeqian; Chen, Chunming; Gong, Shan] Hunan Univ Chinese Med, Dept Pharm, Hosp 1, 95 Shaoshan Middle Rd, Changsha, Hunan, Peoples R China.;[Li, Hong] Second Peoples Hosp Anhui Prov, Dept Pharm, 1868 Dangshan Rd, Hefei, Anhui, Peoples R China.;[Long, Hongping; Long, HP; Liu, Jian] Hunan Univ Chinese Med, Ctr Med Res & Innovat, Hosp 1, 95 Shaoshan Middle Rd, Changsha, Hunan, Peoples R China.;[Ma, Danfeng] Childrens Hosp Hunan Prov, Dept Pharm, 86 Ziyuan Rd, Changsha, Hunan, Peoples R China.
通讯机构：
[Ren, WQ ; Long, HP ] H;Hunan Univ Chinese Med, Dept Pharm, Hosp 1, 95 Shaoshan Middle Rd, Changsha, Hunan, Peoples R China.;Hunan Univ Chinese Med, Ctr Med Res & Innovat, Hosp 1, 95 Shaoshan Middle Rd, Changsha, Hunan, Peoples R China.
关键词：
Introduction;Materials and Methods;Results;Discussion;Conclusion;Abstract;Data Availability;Additional Points;Ethical Approval;Consent;Disclosure;Conflicts of Interests;Authors’ Contributions;Funding Statement;Acknowledgements;Acknowledgments;Supplementary Materials;Reference;Dataset Description;Dataset Files;Abstract;Introduction;Introduction and Materials;Introduction and Methods;Materials;Materials and Methods;Methods;Results;Discussion;Results and Discussion;Discussion and Conclusion;Results and Conclusion;Conclusion;Conclusions;Data Availability;Additional Points;Ethical Approval;Consent;Disclosure;Conflicts of Interest;Authors’ Contributions;Funding Statement;Acknowledgements;Supplementary Materials;References;Appendix;Abbreviations;Preliminaries;Introduction and Preliminaries;Notation;Proof of Theorem;Proofs;Analysis of Results;Examples;Numerical Example;Applications;Numerical Simulation;Model;Model Formulation;Systematic Palaeontology;Nomenclatural Acts;Taxonomic Implications;Experimental;Synthesis;Overview;Characterization;Background;Experimental;Theories;Calculations;Model Verification;Model Implementation;Geographic location;Study Area;Geological setting;Data Collection;Field Testing;Data and Sampling;Dataset;Literature Review;Related Works;Related Work;System Model;Methods and Data;Experimental Results;Results and Analysis;Evaluation;Implementation;Case Presentation;Case Report;Search Terms;Case Description;Case Series;Background;Limitations;Additional Points;Case;Case 1;Case 2 etc.;Concern Details;Retraction Details;Copyright;Related Articles
摘要：
OBJECTIVES: Evodia rutaecarpa (ER) is a well-known herbal Chinese medicine traditionally used for analgesia in dysmenorrhea, headaches, abdominal pain, etc. Notably, the analgesic effect of wine-processed Evodia rutaecarpa (PER) was more potent than that of raw ER. This research aimed to investigate the mechanism and pharmacodynamic substance basis of raw ER and PER on smooth muscle cells of dysmenorrhea mice. METHODS: Metabolomics methods based on UPLC-Q-TOF-MS were utilized to analyse the differential components of ER before and after wine processing. Afterwards, the uterine smooth muscle cells were isolated from the uterine tissue of dysmenorrhea and normal mice. The isolated dysmenorrhea uterine smooth muscle cells were randomly divided into four groups: model group, 7-hydroxycoumarin group (1 mmol/L), chlorogenic acid (1 mmol/L), and limonin (50 μmol/L). The normal group consisted of the isolated normal mouse uterine smooth muscle cells, which were repeated 3 times in each group. The cell contraction and the expression of P2X3 and Ca(2+) in vitro were determined using immunofluorescence staining and laser confocal; ELISA was used for detection of PGE2, ET-1, and NO content after 7-hydroxycoumarin, chlorogenic acid, and limonin administered for 24 h. RESULTS: The metabolomics results suggested that seven differential compounds were identified in the extracts of raw ER and PER, including chlorogenic acid, 7-hydroxycoumarin, hydroxy evodiamine, laudanosine, evollionines A, limonin, and 1-methyl-2-[(z)-4-nonenyl]-4 (1H)-quinolone. The in vitro results showed that 7-hydroxycoumarin, chlorogenic acid, and limonin were able to inhibit cell contraction and PGE2, ET-1, P2X3, and Ca(2+) in dysmenorrhea mouse uterine smooth muscle cells and increase the content of NO. CONCLUSION: Our finding suggested that the compounds of the PER were different from those of the raw ER, and 7-hydroxycoumarin, chlorogenic acid, and limonin could improve dysmenorrhea in mice whose uterine smooth muscle cell contraction was closed with endocrine factors and P2X3-Ca(2+) pathway.
作者机构：
[Liu, Jianjun; Lei, Chang; Zhou, Wenchao; Zhou, Wei; Huang, Dan; Li, Shunxiang] Hunan Univ Chinese Med, Sci & Technol Innovat Ctr, State Key Lab Chinese Med Powder & Med Innovat Hun, Changsha 410208, Peoples R China.;[Huang, Dan; Li, Shunxiang] Hunan Univ Chinese Med, Hunan Engn Technol Res Ctr Bioact Subst Discovery, Sch Pharm, Changsha 410208, Peoples R China.;[Huang, Dan; Li, Shunxiang] Hunan Prov Sino US Int Joint Res Ctr Therapeut Dru, Changsha 410208, Peoples R China.;[Huang, Dan; Li, Shunxiang] Hunan Univ Chinese Med, Sci & Technol Innovat Ctr, State Key Lab Chinese Med Powder & Med Innovat Hun, Changsha 410208, Peoples R China.;[Huang, Dan; Li, Shunxiang] Hunan Univ Chinese Med, Hunan Engn Technol Res Ctr Bioact Subst Discovery, Sch Pharm, Changsha 410208, Peoples R China.
通讯机构：
[Huang, D ; Li, SX] ;Hunan Univ Chinese Med, Sci & Technol Innovat Ctr, State Key Lab Chinese Med Powder & Med Innovat Hun, Changsha 410208, Peoples R China.;Hunan Univ Chinese Med, Hunan Engn Technol Res Ctr Bioact Subst Discovery, Sch Pharm, Changsha 410208, Peoples R China.;Hunan Prov Sino US Int Joint Res Ctr Therapeut Dru, Changsha 410208, Peoples R China.
摘要：
Abstract: The gas chromatography–ion mobility spectrometry (GC-IMS) method is a new technology for detecting volatile organic compounds. This study was carried out to evaluate the effects of volatile aroma compounds of Curcuma essential oils (EOs) after 60Co radiation by GC-IMS. Dosages of 0, 5, and 10 kGy of 60Co were used to analyze EOs of Curcuma after 60Co irradiation (named EZ-1, EZ-2, and EZ-3). The odor fingerprints of volatile organic compounds in different EOs of Curcuma samples were constructed by headspace solid-phase microextraction and GC-IMS after irradiation. The differences in odor fingerprints of EOs were compared by principal component analysis (PCA). A total of 92 compounds were detected and 65 compounds were identified, most of which were ketones, aldehydes, esters, and a small portion were furan compounds. It was found that the volatile matter content of 0 kGy and 5 kGy was closer, and the use of 10 kGy 60Co irradiation would have an unstable effect on the EOs. In summary, it is not advisable to use a higher dose when using 60Co irradiation for sterilization of Curcuma. Due to the small gradient of irradiation dose used in the experiment, the irradiation dose can be adjusted appropriately according to the required sterilization requirements during the production and storage process of Curcuma to obtain the best irradiation conditions. GC-IMS has the advantages of GC’s high separation capability and IMS’s fast response, high resolution, and high sensitivity, and the sample requires almost no pretreatment; it can be widely used in the analysis of traditional Chinese medicines containing volatile components. It is shown that irradiation technology has good application prospects in the sterilization of traditional Chinese medicines, but the changes in irradiation dose and chemical composition must be paid attention to. Keywords: Curcuma; essential oils; chemical compounds; 60Co irradiation; GC-IMS
通讯机构：
[Zhou, RiBao; Liu, XiangDan] C;College of Pharmacy, Hunan University of Chinese Medicine, Changsha, 410208, China.;Key Laboratory of Germplasm Resources and Standardized Planting of Hunan Large-Scale Genuine Medicinal Materials, Changsha, 410208, China.;Key Laboratory of Modern Research of TCM, Education Department of Hunan Province, Changsha, 410208, China.
摘要：
BACKGROUND: Lonicera macranthoides Hand.-Mazz. is an important medicinal plant. Xianglei-type (XL) L. macranthoides was formed after many years of cultivation by researchers on the basis of the natural mutant. The corolla of L. macranthoides XL remains unexpanded and its flowering period is nearly three times longer than that of wild-type (WT) plants. However, the molecular mechanism behind this desirable trait remains a mystery. OBJECTIVE: To understand the floral phenotype differences between L. macranthoides and L. macranthoides XL at the molecular level. METHODS: Transcriptome analysis was performed on L. macranthoides XL and WT. One DEG was cloned by RT-PCR amplification and selected for qRT-PCR analysis. RESULTS: Transcriptome analysis showed that there were 5603 differentially expressed genes (DEGs) in XL vs. WT. Enrichment analysis of DEGs showed that pathways related to plant hormone signal transduction were significantly enriched. We identified 23 key genes in ethylene biosynthesis and signal transduction pathways. The most abundant were the ethylene biosynthesis DEGs. In addition, the open reading frames (ORFs) of WT and XL ETR2 were successfully cloned and named LM-ETR2 (GenBank: MW334978) and LM-XL-ETR2 (GenBank: MW334978), respectively. qRT-PCR at different flowering stages suggesting that ETR2 acts in the whole stage of flower development of WT and XL. CONCLUSIONS: This study provides new insight into the molecular mechanism that regulates the development of special traits in the flowers of L. macranthoides XL. The plant hormone ethylene plays an important role in flower development and flowering duration prolongation in L. macranthoides. The ethylene synthesis gene could be more responsible for the flower phenotype of XL. The genes identified here can be used for breeding and improvement of other flowering plants after functional verification.
期刊：
International Journal of Molecular Sciences,2023年24(8) ISSN：1422-0067
通讯作者：
Naihong Chen<&wdkj&>Songwei Yang
作者机构：
[Ai, Qidi; Lin, Meiyu; Yao, Jiao; Yang, Songwei; Liu, Xinya; Guo, Yi; Yang, Yantao; Chen, Naihong] Hunan Univ Chinese Med, Coll Pharm, Hunan Engn Technol Ctr Standardizat & Funct Chines, Changsha 410208, Peoples R China.;[Ai, Qidi; Yao, Jiao; Yang, Songwei] TCM, Educ Dept Hunan Prov, Key Lab Modern Res, Changsha 410208, Peoples R China.;[Chen, Cong] Hunan Univ Chinese Med, Sch Tradit Chinese Med, Changsha 410208, Peoples R China.;[Guo, Yi] Hunan Univ Chinese Med, Sch Acupuncture & Tuina & Rehabil, Changsha 410208, Peoples R China.;[Chen, Naihong; Zhang, Zhao; Chu, Shifeng] Chinese Acad Med Sci & Peking Union Med Coll, Inst Mat Med, State Key Lab Bioact Subst & Funct Nat Med, Beijing 100050, Peoples R China.
通讯机构：
[Naihong Chen; Songwei Yang] H;Hunan Engineering Technology Center of Standardization and Function of Chinese Herbal Decoction Pieces, College of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China<&wdkj&>State Key Laboratory of Bioactive Substances and Functions of Natural Medicines, Institute of Materia Medica & Neuroscience Center, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China<&wdkj&>Hunan Engineering Technology Center of Standardization and Function of Chinese Herbal Decoction Pieces, College of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China<&wdkj&>Key Laboratory of Modern Research of TCM, Education Department of Hunan Province, Changsha 410208, China
摘要：
Abstract: Depression is a mental illness that has a serious negative impact on physical and mental health. The pathophysiology of depression is still unknown, and therapeutic medications have drawbacks, such as poor effectiveness, strong dependence, adverse drug withdrawal symptoms, and harmful side effects. Therefore, the primary purpose of contemporary research is to understand the exact pathophysiology of depression. The connection between astrocytes, neurons, and their interactions with depression has recently become the focus of great research interest. This review summarizes the pathological changes of neurons and astrocytes, and their interactions in depression, including the alterations of mid-spiny neurons and pyramidal neurons, the alterations of astrocyte-related biomarkers, and the alterations of gliotransmitters between astrocytes and neurons. In addition to providing the subjects of this research and suggestions for the pathogenesis and treatment techniques of depression, the intention of this article is to more clearly identify links between neuronal–astrocyte signaling processes and depressive symptoms. Keywords: depression; neurons; astrocytes; astrocyte–neuron interactions
作者机构：
[Qiu, Yi-xing; Li, Bin; Wang, Wei; Wang, Hui-zhen; Yang, Yu-pei; Yu, Huang-he; Jian, Yu-qing; Liu, Shi-qi] Hunan Univ Chinese Med, Sch Pharm, TCM & Ethnomedicine Innovat & Dev Int Lab, Changsha 410208, Peoples R China.;[Hussain, Nusrat] Univ Baltistan Skardu, Dept Chem, Skardu 16100, Pakistan.;[Wang, Wei] Hunan Univ Chinese Med, Sch Pharm, TCM & Ethnomedicine Innovat & Dev Int Lab, Changsha 410208, Hunan, Peoples R China.
通讯机构：
[Wang, W ] H;Hunan Univ Chinese Med, Sch Pharm, TCM & Ethnomedicine Innovat & Dev Int Lab, Changsha 410208, Hunan, Peoples R China.
关键词：
Schisandraceae;Dibenzocyclooctadiene lignans;Liver injury;Structure-activity relationship;Absorption or permeation properties
摘要：
Liver injury is a common pathological process characterized by massive degeneration and abnormal death of liver cells. With increase in dead cells and necrosis, liver injury eventually leads to nonalcoholic fatty liver disease (NAFLD), hepatic fibrosis, and even hepatocellular carcinoma (HCC). Consequently, it is necessary to treat liver injury and to prevent its progression. The drug Bicylol is widely employed in China to treat chronic hepatitis B virus (HBV) and has therapeutic potential for liver injury. It is the derivative of dibenzocyclooctadiene lignans extracted from Schisandra chinensis (SC). The Schisandraceae family is a rich source of dibenzocyclooctadiene lignans, which possesses potential liver protective activity. This study aimed to comprehensively summarize the phytochemistry, structure-activity relationship and molecular mechanisms underlying the liver protective activities of dibenzocyclooctadiene lignans from the Schisandraceae family. Here, we had discussed the analysis of absorption or permeation properties of 358 compounds based on Lipinski's rule of five. So far, 358 dibenzocyclooctadiene lignans have been reported, with 37 of them exhibited hepatoprotective effects. The molecular mechanism of the active compounds mainly involves antioxidative stress, anti-inflammation and autophagy through Kelch-like ECH-associating protein 1/nuclear factor erythroid 2 related factor 2/antioxidant response element (Keap1/Nrf2/ARE), nuclear factor kappa B (NF-кB), and transforming growth factor β (TGF-β)/Smad 2/3 signaling pathways. This review is expected to provide scientific ideas for future research related to developing and utilizing the dibenzocyclooctadiene lignans from Schisandraceae family.
摘要：
BackgroundThere is growing evidence demonstrating that the gut microbiota plays a crucial role in multiple endocrine disorders, including diabetic cardiomyopathy (DCM). Research shows that the Chinese herb reduces disease occurrence by regulating gut microbiota. Zuogui Jiangtang Shuxin formula (ZGJTSXF), a Chinese medicinal formula, has been clinically used for treatment of DCM for many years. However, there is still no clear understanding of how ZGJTSXF treatment contributes to the prevention and treatment of DCM through its interaction with gut microbiota and metabolism. MethodsIn this study, mice models of DCM were established, and ZGJTSXF's therapeutic effects were assessed. Specifically, serum glycolipid, echocardiography, histological staining, myocardial apoptosis rate were assessed. Using 16s rRNA sequencing and high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS), we determined the impact of ZGJTSXF on the structure of gut microbiota and content of its metabolite TMAO. The mechanism of ZGJTSXF action on DCM was analyzed using quantitative real-time PCR and western blots. ResultsWe found that ZGJTSXF significantly ameliorated DCM mice by modulating gut-heart axis: ZGJTSXF administration improved glycolipid levels, heart function, cardiac morphological changes, inhibited cardiomyocytes apoptosis, and regulate the gut microbiota in DCM mice. Specifically, ZGJTSXF treatment reverse the significant changes in the abundance of certain genera closely related to DCM phenotype, including Lactobacillus, Alloprevotella and Alistipes. Furthermore, ZGJTSXF alleviated DCM in mice by blunting TMAO/PERK/FoxO1 signaling pathway genes and proteins. ConclusionZGJTSXF administration could ameliorate DCM mice by remodeling gut microbiota structure, reducing serum TMAO generation and suppressing TMAO/PERK/FoxO1 signaling pathway.
期刊：
Frontiers in Microbiology,2023年14:1157475 ISSN：1664-302X
通讯作者：
Peng, Maijiao;Xiao, NQ
作者机构：
[Xiao, Nenqun; Zhou, Kang; Peng, Maijiao; Peng, MJ; Xiao, NQ; Yi, Xin] Hunan Univ Chinese Med, Coll Pharm, Changsha, Peoples R China.;[Tan, Zhoujin] Hunan Univ Chinese Med, Coll Chinese Med, Changsha, Peoples R China.
通讯机构：
[Peng, MJ; Xiao, NQ ] H;Hunan Univ Chinese Med, Coll Pharm, Changsha, Peoples R China.
关键词：
Baohe pill decoction;diarrhea;high-fat and high-protein diet;intestinal musoca;lactase-producing bacteria
摘要：
INTRODUCTION: This study aimed to investigate the effects of Baohe pill decoction (BPD) on microbial, lactase activity, and lactase-producing bacteria in the intestinal mucosa of mice with diarrhea induced by high-fat and high-protein diet (HFHPD). METHODS: Thirty male Kunming (KM) mice were randomly divided into normal (NM), model (MD), and BPD groups. Diarrhea models were manufactured using HFHPD combined with a gavage of vegetable oil. At the end of modeling, the BPD group was given BPD (6.63 g·kg(-1)d(-1)) intervention twice daily for 3 d. The NM and MD groups were given equal amounts of sterile water. Subsequently, the intestinal mucosa of the mice was collected, one portion was used for microbial and lactase activity measurement, and the other portion was used for its lactase-producing bacterial characteristics by high-throughput sequencing technology. RESULTS: Our results showed that microbial and lactase activity of intestinal mucosa decreased significantly following diarrhea in mice (P(microbial) < 0.05, P(lactase) < 0.001). After BPD intervention, microbial and lactase activity increased significantly (P < 0.01). The number of operational taxonomic units (OTUs), richness, and diversity index of lactase-producing bacteria increased in the BPD group compared to the MD group (P > 0.05), and the community structure were significant differences (P < 0.01). Compared to other groups, Saccharopolyspora, Rhizobium, Cedecea, and Escherichia were enriched in the BPD group. Notably, the relative abundance of the dominant lactase-producing genus Bifidobacterium decreased after BPD intervention. DISCUSSION: The mechanism of BPD in relieving diarrhea induced by HFHPD is closely related to the promotion of lactase activity in the intestinal mucosa, which may be achieved by regulating the structure of lactase-producing bacteria.
作者机构：
[Ai, Qidi; Chen, Haodong; Feng, Juling; Yang, Yantao; Chen, Naihong; Liu, Yangbo; Chen, NH] Hunan Univ Chinese Med, Coll Pharm, Hunan Engn Technol Ctr Standardizat & Funct Chines, Changsha 410208, Peoples R China.;[Wang, Hongyun; Chen, Naihong; Chu, Shifeng; Zhang, Zhao; Chen, NH] Chinese Acad Med Sci & Peking Union Med Coll, Inst Mat Med, State Key Lab Bioact Subst & Funct Nat Med, Xian Nong Tan St, Beijing 100050, Peoples R China.;[Wang, Hongyun; Chen, Naihong; Chu, Shifeng; Zhang, Zhao; Chen, NH] Chinese Acad Med Sci & Peking Union Med Coll, Neurosci Ctr, Xian Nong Tan St, Beijing 100050, Peoples R China.
通讯机构：
[Chu, SF] C;[Chen, NH ] H;Hunan Univ Chinese Med, Coll Pharm, Hunan Engn Technol Ctr Standardizat & Funct Chines, Changsha 410208, Peoples R China.;Chinese Acad Med Sci & Peking Union Med Coll, Inst Mat Med, State Key Lab Bioact Subst & Funct Nat Med, Xian Nong Tan St, Beijing 100050, Peoples R China.;Chinese Acad Med Sci & Peking Union Med Coll, Neurosci Ctr, Xian Nong Tan St, Beijing 100050, Peoples R China.
摘要：
Anti-oxidant stress is a potential strategy for the treatment of ischemic stroke. Here, we found a novel free radical scavenger termed as CZK, which is derived from alkaloids contained in Clausena lansium. In this study, we first compared cytotoxicity and biological activity between CZK and its parent's compound Claulansine F. It was found that CZK had lower cytotoxicity and improved anti-oxygen-glucose deprivation/reoxygenation (OGD/R) injury than its parent's compound. Free radical scavenging test showed that CZK had a strong inhibitory effect on hydroxyl free radicals with the IC50 of 77.08 nM. Intravenous injection of CZK (50 mg/kg) significantly alleviated ischemia-reperfusion injury, manifested by reduced neuronal damage and decreased oxidative stress. Consistent with the findings, the activities of superoxide dismutase (SOD) and reduced glutathione (GSH) were increased. Molecular docking predicted that CZK might be combined with nuclear factor erythroid 2-related factor 2 (Nrf2) complex. Our results also confirmed that CZK upregulated the contents of Nrf2 and its target gene products Heme Oxygenase-1 (HO-1), and NAD(P)H: Quinone Oxidoreductase 1 (NQO1). In conclusion, CZK had a potential therapeutic effect for ischemic stroke by activating Nrf2-mediated antioxidant system.
