作者机构：
[朱莹] Department of Gastroenterology, The First Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China;[胡卓瑜] Graduate School, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;[邹孟龙; 龙丹; 孙豪娴] Department of Gastroenterology, The First Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China<&wdkj&>Graduate School, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China
通讯机构：
[Zhu Ying] D;Department of Gastroenterology, The First Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China
摘要：
Objective To evaluate the methodological quality of papers that performed meta-analyzed and systematically reviewed acupoint selections for the treatment of functional dyspepsia(FD)and to identify the ideal acupoint combinations for FD.Methods Chinese databases includin...MORE Objective To evaluate the methodological quality of papers that performed meta-analyzed and systematically reviewed acupoint selections for the treatment of functional dyspepsia(FD)and to identify the ideal acupoint combinations for FD.Methods Chinese databases including China National Knowledge Infrastructure(CNKI),China Science and Technology Journal Database(VIP),China Biology Medicine(CBM),and Wanfang Database,as well as English databases including PubMed,Embase,and Cochrane Library were searched to retrieve papers about meta-analysis and systematic literature reviews on acupuncture for FD.The time span for the paper retrieval was set from the foundation of the databases to April 30,2022.The Veritas scores of the papers based on their publication year,study type,Assessment of Multiple Systematic Reviews 2(AMSTAR2),Preferred Reporting Items for Systematic Reviews and Meta-Analyses(PRISMA),heterogeneity,and publication bias were rated to assess the methodological quality of the included studies.Then,randomized controlled trials(RCTs)were extracted from those meta-analysis papers or systematic literature reviews for analyzing acupoints frequency,meridian frequency,and association rules with the use of R software(V 4.3.1).Results Eight meta-analysis papers were included in the study after screening.The mean Veritas scores of the papers based on publication year,type of study,AMSTAR2,PRISMA,heterogeneity,and publication bias were 4.50,8.00,4.63,4.63,4.50,and 6.13,respectively.The analysis of the scores revealed insufficiencies in the reviews pertaining to the methodology,comprehension of the research strategy,detailed list of excluded studies,sources of funding,assessment of potential bias risks impact on meta-analysis results in each study,explanation of heterogeneity,and identification of potential conflicts of interest.Furthermore,a total of 85 RCTs were obtained from the eight meta-analysis papers involving 85 acupuncture prescriptions and 67 acupoints for subsequent data mining.The most commonly used meridian was Stomach meridian of Foot-Yangming(ST).Zusanli(ST36),Neiguan(PC6),Zhongwan(CV12),Taichong(LR3),Tianshu(ST25),Gongsun(SP4),Weishu(BL21),Pishu(BL20),Neiting(ST44),and Yinlingquan(SP9)topped the list of frequently selected acupoints.Additionally,a total of 28 association rules were identified,including 10 second-order,15 third-order,and 3 fourth-order association rules.The top-ranking association rules in each order were“Neiguan(PC6)→Zusanli(ST36)”“Zhongwan(CV12)+Neiguan(PC6)→Zusanli(ST36)”and“Zhongwan(CV12)+Taichong(LR3)+Neiguan(PC6)→Zusanli(ST36)”,respectively.Conclusion Acupuncture could alleviate the clinical symptoms of FD.However,the quality of methodology applied in the meta-analysis papers on the subject needs to be improved.Through data mining,a combination of Neiguan(PC6),Zusanli(ST36),Zhongwan(CV12),and Taichong(LR3)was identified as an essential acupoint combination for the treatment of FD.FEWER
摘要：
This study investigated whether liquiritin can alleviate cerebral ischemia-reperfusion injury by regulating Nurr1 to mediate mitochondrial homeostasis. SH-SY5Y cells were subjected to glucose deprivation and reperfusion to establish a cerebral ischemia-reperfusion injury model in vitro. Cell viability and apoptosis were then determined using a cell counting kit and flow cytometry analysis. The degree of mitochondrial swelling was evaluated using a cell mitochondria isolation kit. Reactive superoxide generation, mitochondrial membrane potential, adenosine triphosphate (ATP) content, and mitochondrial ultrastructure were analyzed using dihydroethidium, JC-1 (5,5 ',6,6 '-tetrachloro1,1 ',3,3 '-tetramethylbenzimidazolylcarbocyanine iodide), luciferase-based ATP bioluminescent assays, and transmission electron microscopy, respectively. Quantitative reverse transcription PCR and western blot assays were conducted to detect levels of mitochondrial fission-related factors. Glucose deprivation and reperfusion exposure significantly reduced the viability and induced apoptosis of SH-SY5Y cells, indicating that glucose deprivation and reperfusion exposure successfully induced cerebral ischemia-reperfusion injury. Glucose deprivation and reperfusion exposure also increased the degree of mitochondrial swelling, promoted an increase in superoxide, and decreased mitochondrial membrane potential and ATP enzyme levels. Cerebral ischemia-reperfusion injury also significantly increases Drp1 and Fis1 protein expression, reduces mitofusin-2 and optic atrophy 1 levels, increases nuclear receptor-related 1 and inverted formin-2 expression, and decreases yes-associated protein expression. Electron microscopy further revealed sparse mitochondria and broken cristae. However, these findings were reversed by liquiritin in a dose-dependent manner and were further abolished after carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone treatment. Our study suggests that the protective effects of liquiritin on cerebral ischemia-reperfusion injury are linked to nuclear receptor-related 1 upregulation, followed by the regulation of yes-associated protein-inverted formin-2-mitochondrial fission pathways. Liquiritin may represent a novel therapeutic agent for treating cerebral ischemia-reperfusion injury.
作者机构：
湖南中医药大学 研究生院,湖南 长沙, 410036;湖南省中医药研究院,中药资源研究所,湖南 长沙, 410013;绿之韵生物工程集团有限公司,湖南 长沙,410329;[金剑; 张水寒; 钟灿] Institute of Chinese Medicine Resources, Hunan Academy of Chinese Medicine, Changsha, 410013, China;[贺炜; 劳嘉] Resgreen Group International Inc., Changsha, 410329, China
通讯机构：
[Jin, J.] I;Institute of Chinese Medicine Resources, China
摘要：
股骨头坏死(osteonecrosis of the femoral head, ONFH)是一种常见的骨科疾病，发病机制复杂，目前临床上的治疗方法并不令人满意。外泌体是细胞分泌的具有双层膜的细胞外囊泡，内含丰富的蛋白质与核酸分子。外泌体在血清中的含量及外泌体内miRNA的差异性能够反映ONFH的发生阶段；而不同细胞来源的外泌体能够通过调控信号传导和相关基因表达影响骨细胞、血管细胞的增殖分化，进而影响骨重塑。因此，外泌体在ONFH的诊治方面具有重要的应用价值。本文对外泌体进行了概述，对外泌体在ONFH诊断和治疗中的应用价值进行了综述。
摘要：
The circadian rhythm is an endogenous clock system that coordinates and optimizes various physiological and pathophysiological processes, which accord with the master and the peripheral clock. Increasing evidence indicates that endogenous circadian rhythm disruption is involved in the lesion volume and recovery of ischemic stroke. As a critical recovery mechanism in post-stroke, angiogenesis reestablishes the regional blood supply and enhances cognitive and behavioral abilities, which is mainly composed of the following processes: endothelial cell proliferation, migration, and pericyte recruitment. The available evidence revealed that the circadian governs many aspects of angiogenesis. This study reviews the mechanism by which circadian rhythms regulate the process of angiogenesis and its contribution to functional recovery in post-stroke at the aspects of the molecular level. A comprehensive understanding of the circadian clock regulating angiogenesis in post-stroke is expected to develop new strategies for the treatment of cerebral infarction.
摘要：
Background: Knee osteoarthritis (KOA), a chronic degenerative disease, is mainly characterized by destruction of articular cartilage and inflammatory reactions. At present, there is a lack of economical and effective clinical treatment. Zhuifeng Tougu (ZFTG) capsules have been clinically approved for treatment of OA as they relieve joint pain and inflammatory manifestations. However, the mechanism of ZFTG in KOA remains unknown. Purpose: This study aimed to investigate the effect of ZFTG on the TLR4/MyD88/NF-kappa B signaling pathway and its therapeutic effect on rabbits with KOA. Study design: In vivo, we established a rabbit KOA model using the modified Videman method. In vitro, we treated chondrocytes with IL-1 beta to induce a pro-inflammatory phenotype and then intervened with different concentrations of ZFTG. Levels of IL-1 beta, IL-6, TNF-alpha, and IFN-gamma were assessed with histological observations and ELISA data. The effect of ZFTG on the viability of chondrocytes was detected using a Cell Counting Kit-8 and flow cytometry. The protein and mRNA expressions of TLR2, TLR4, MyD88, and NF-kappa B were detected using Western blot and RT-qPCR and immunofluorescence observation of NF-kappa B p65 protein expression, respectively, to investigate the mechanism of ZFTG in inhibiting inflammatory injury of rabbit articular chondrocytes and alleviating cartilage degeneration. Results: The TLR4/MyD88/NF-kappa B signaling pathway in rabbits with KOA was inhibited, and the levels of IL-1 beta, IL-6, TNF-alpha, and IFN-gamma in blood and cell were significantly downregulated, consistent with histological results. Both the protein and mRNA expressions of TLR2, TLR4, MyD88, NF-kappa B, and NF-kappa B p65 proteins in that nucleus decreased in the ZFTG groups. Moreover, ZFTG promotes the survival of chondrocytes and inhibits the apoptosis of inflammatory chondrocytes. Conclusion: ZFTG alleviates the degeneration of rabbit knee joint cartilage, inhibits the apoptosis of inflammatory chondrocytes, and promotes the survival of chondrocytes. The underlying mechanism may be inhibition of the TLR4/MyD88/NF-kB signaling pathway and secretion of inflammatory factors.
作者机构：
[方闯; 匡泓俊; 曹洋; 夏叶婉] Postgraduate School of Hunan University of Chinese Medicine, Changsha 410208, China;[钟峰; 章薇; 罗容] Department of Acupuncture and Tuina Rehabilitation, The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha 410007;[文钱] Department of Traditional Chinese Medicine, Huaihua Hospital of Traditional Chinese Medicine, Huaihua 418099, Hunan Province
