摘要：
Background: QiShenYiQi Pills (R) (QSYQ) is a compound Chinese medicine used in China for alleviating cardiac function. The present study was designed to explore the effect and mechanism of QSYQ on ischemia-reperfusion (I/R)-induced disorders in myocardial structure and function, with particularly focusing on the regulation of energy metabolism. Methods: Sprague-Dawley rats, with or without QSYQ pretreatment, were subjected to 30 min occlusion of the left anterior descending coronary artery and followed by 90 min or 24 h reperfusion. Myocardial blood flow (MBF) and cardiac function were evaluated at baseline, immediately after ischemia and 30, 60, 90 min, and 24 h after reperfusion. Myocardial infarction, myocardial histology and ultrastructure were assessed. Double staining of alpha-cardiac actinin and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling was conducted to assess myocardial apoptosis. ATP, ADP and AMP content was determined by Enzyme-Linked Immunosorbent Assay, F-actin in myocardial cells determined by immunofluorescence microscopy and expression of ATP synthase alpha, ATP5D, and phosphorylated-Myosin Light Chain (P-MLC) determined by western blotting. Results: Pre-treatment with QSYQ protected against I/R-induced MBF decrease, myocardial infarction and apoptosis at 90 min and 24 h after reperfusion. Moreover, I/R 90 min caused an impairment on cardiac function, a decrease in the ratio of ADP/ATP and AMP/ATP, accompanying with reduction of ATP 5D expression and increase in the expression of P-MLC, meanwhile, myocardium to exhibit myocardial fiber rupture, interstitial edema, and mitochondria swelling, all of which were significantly ameliorated by pre-treatment with QSYQ. Conclusions: The results of the present study suggest an involvement of regulation of energy metabolism in the action of QSYQ to protect against myocardial I/R injury. (C) 2012 Elsevier Ireland Ltd. All rights reserved.
期刊：
MOLECULAR MEDICINE REPORTS,2015年12(3):4273-4283 ISSN：1791-2997
通讯作者：
Tang, Nong
作者机构：
[Li, Tian-Wei; Bi, Xin-Ya; Tang, Nong; Cai, Xin-Kun; Wang, Qing-Bi; Li, Hai-Yuan; Wu, Lin] Guangxi Univ Chinese Med, Guangxi Sci Expt Ctr Tradit Chinese Med, Nanning 530001, Guangxi, Peoples R China.;[Tang, Nong; Wu, Lin] Guangxi Univ Chinese Med, Guangxi Key Lab Chinese Med Fdn Res, Nanning 530001, Guangxi, Peoples R China.;[Tang, Nong] Guangxi Univ Chinese Med, Dept Internal Neurol, Affiliated Hosp 1, Nanning 530023, Guangxi, Peoples R China.;[Tang, Nong] Guangxi Univ Chinese Med, Guangxi Sci Expt Ctr Tradit Chinese Med, 179 East Mingxiu Rd, Nanning 530001, Guangxi, Peoples R China.;[Zhao, Qing-Shan] Hunan Univ Chinese Med, Grad Sch, Changsha 410208, Hunan, Peoples R China.
通讯机构：
[Tang, Nong] Guangxi Univ Chinese Med, Guangxi Sci Expt Ctr Tradit Chinese Med, 179 East Mingxiu Rd, Nanning 530001, Guangxi, Peoples R China.
关键词：
Chinese medicine;vascular dementia;memory impairment;ischemia/reperfusion;apoptosis;signal transduction
摘要：
Apoptosis and the dysfunction of the cyclic adenosine monophosphate (cAMP)/protein kinase A (PKA)/cAMP-responsive element binding protein (CREB) signaling pathway have a key role in memory impairment in vascular dementia (VaD), a challenging clinical problem. Yifei Xuanfei Jiangzhuo formula (YXJF), a Chinese herbal decoction, has been used to treat VaD in clinical practice and has produced positive outcomes; however, convincing evidence is currently lacking. The present study aimed to investigate the effects of YXJF on memory impairment in rats with cerebral ischemia/reperfusion and to explore the underlying mechanism. YXJF ameliorated memory impairment in rats with cerebral ischemia/reperfusion, inhibited hippocampal apoptosis in a dose-dependent manner and attenuated increases in the protein expression of B-cell lymphoma 2 (Bcl-2)-associated X protein as well as c-Jun and a reduction in Bcl-2 protein expression in the hippocampal tissue of the rats. Furthermore, administration of YXJF significantly increased the protein expression of PKA C-alpha and CREB, and promoted CREB phosphorylation. The results indicated that YXJF improves memory impairment through inhibiting apoptosis and enhancing PKA/CREB signal transduction in rats with cerebral ischemia/reperfusion.
期刊：
Frontiers of Medicine,2015年9(1):20-29 ISSN：2095-0217
通讯作者：
Hu, Guoheng
作者机构：
[Li Yingchen; Cheng Qilai] Post-Graduate School, Hunan University of Traditional Chinese Medicine;[Hu Guoheng] Department of Neurology, The First Affiliated Hospital of Hunan University of Traditional Chinese Medicine
通讯机构：
[Hu, Guoheng] Hunan Univ Tradit Chinese Med, Dept Neurol, Affiliated Hosp 1, Changsha 410007, Hunan, Peoples R China.
关键词：
human umbilical cord;mesenchymal stem cells;ischemic stroke;cellular therapy;transplantation
摘要：
Ischemic stroke is a focal cerebral insult that often leads to many adverse neurological complications severely affecting the quality of life. The prevalence of stroke is increasing throughout the world, while the efficacy of current pharmacological therapies remains unclear. As a neuroregenerative therapy, the implantation of human umbilical cord mesenchymal stem cells (hUC-MSCs) has shown great possibility to restore function after stroke. This review article provides an update role of hUC-MSCs implantation in the treatment of ischemic stroke. With the unique “immunosuppressive and immunoprivilege” property, hUC-MSCs are advised to be an important candidate for allogeneic cell treatment. Nevertheless, most of the treatments are still at primary stage and not clinically feasible at the current time. Several uncertain problems, such as culture conditions, allograft rejection, and potential tumorigenicity, are the choke points in this cellular therapy. More preclinical researches and clinical studies are needed before hUC-MSCs implantation can be used as a routinely applied clinical therapy.
摘要：
Vascular dementia (VD) has been one of the most serious public health problems worldwide. It is well known that cerebral hypoperfusion is the key pathophysiological basis of VD, but it remains unclear how global genes in hippocampus respond to cerebral ischemia-reperfusion. In this study, we aimed to reveal the global gene expression profile in the hippocannpus of VD using a rat model. VD was induced by repeated occlusion of common carotid arteries followed by reperfusion. The rats with VD were characterized by deficit of memory and cognitive function and by the histopathological changes in the hippocampus, such as a reduction in the number and the size of neurons accompanied by an increase in intercellular space. Microarray analysis of global genes displayed up-regulation of 7 probesets with genes with fold change more than 1.5 (P < 0.05) and down-regulation of 13 probesets with genes with fold change less than 0.667 (P < 0.05) in the hippocampus. Gene Ontology (GO) and pathway analysis showed that the up-regulated genes are mainly involved in oxygen binding and transport, autoimmune response and inflammation, and that the down-regulated genes are related to glucose metabolism, autoimmune response and inflammation, and other biological process, related to memory and cognitive function. Thus, the abnormally expressed genes are closely related to oxygen transport, glucose metabolism, and autoimmune response. The current findings display global gene expression profile of the hippocampus in a rat model of VD, providing new insights into the molecular pathogenesis of VD.
作者机构：
[Chai, Qiu-Ye] Research Center for Marine Drugs, Department of Pharmacy, State Key Laboratory of Oncogenes and Related Genes, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China. chaiqiuye123@sina.cn;[Chai, Qiu-Ye] School of Pharmacy, Jiangxi University of Traditional Chinese Medicine, Nanchang 330000, China. chaiqiuye123@sina.cn;[Yang, Zhen] Department of Pharmacy, Graduate School, Hunan University of Chinese Medicine, Changsha 410208, China. zyyangzhen1991@163.com;[Han, Bing-Nan] Research Center for Marine Drugs, Department of Pharmacy, State Key Laboratory of Oncogenes and Related Genes, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China. hanbingnan@shsmu.edu.cn;[Lin, Hou-Wen] Research Center for Marine Drugs, Department of Pharmacy, State Key Laboratory of Oncogenes and Related Genes, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China. franklin67@126.com
通讯机构：
[Lin, Hou-Wen; Han, Bing-Nan; Chai, Qiu-Ye] Research Center for Marine Drugs, Department of Pharmacy, State Key Laboratory of Oncogenes and Related Genes, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China.;[Yang, Zhen] Department of Pharmacy, Graduate School, Hunan University of Chinese Medicine, Changsha 410208, China.;[Chai, Qiu-Ye] School of Pharmacy, Jiangxi University of Traditional Chinese Medicine, Nanchang 330000, China.
摘要：
Since the 1990s, a number of terminal alkynyl residue-containing cyclic/acyclic peptides have been identified from marine organisms, especially cyanobacteria and marine mollusks. This review has presented 66 peptides, which covers over 90% marine peptides with terminal alkynyl fatty acyl units. In fact, more than 90% of these peptides described in the literature are of cyanobacterial origin. Interestingly, all the linear peptides featured with terminal alkyne were solely discovered from marine cyanobacteria. The objective of this article is to provide an overview on the types, structural characterization of these unusual terminal alkynyl fatty acyl units, as well as the sources and biological functions of their composed peptides. Many of these peptides have a variety of biological activities, including antitumor, antibacterial, antimalarial, etc. Further, we have also discussed the evident biosynthetic origin responsible for formation of terminal alkynes of natural PKS (polyketide synthase)/NRPS (nonribosome peptide synthetase) hybrids.
期刊：
EXPERIMENTAL AND THERAPEUTIC MEDICINE,2013年6(2):347-354 ISSN：1792-0981
通讯作者：
Tang, Jin-Tian
作者机构：
[Li, Li; Tang, Jin-Tian] Cent S Univ, Dept Oncol, Xiangya Hosp, Changsha 410008, Hunan, Peoples R China.;[Li, Li; Tang, Jin-Tian; Kong, Wei-Chao; Xie, Le; Shi, Huan-Huan; Li, Jing-Ding-Sha; Zhao, Ling-Yun; Wang, Rui] Tsinghua Univ, Inst Med Phys & Engn, Key Lab Particle & Radiat Imaging, Minist Educ,Dept Engn Phys, Beijing 100084, Peoples R China.;[Tang, Jin-Tian] Tsinghua Univ, Dept Engn Phys, Room B116,Bldg Med Sci, Beijing 100084, Peoples R China.;[Kong, Wei-Chao; Shi, Huan-Huan; Li, Jing-Ding-Sha] Beijing Univ Chinese Med, Dept Biopharmaceut, Beijing 100102, Peoples R China.;[Xie, Le] Hunan Univ Chinese Med, Grad Sch, Changsha 410208, Hunan, Peoples R China.
通讯机构：
[Tang, Jin-Tian] Tsinghua Univ, Dept Engn Phys, Room B116,Bldg Med Sci, Beijing 100084, Peoples R China.
关键词：
magnetic stent hyperthermia;percutaneous transluminal coronary angioplasty;restenosis;vascular smooth muscle cells;alternative magnetic field
摘要：
Thermal treatment or hyperthermia has received considerable attention in recent years due to its high efficiency, safety and relatively few side-effects. In this study, we investigated whether it was possible to utilize targeted thermal or in-stent thermal treatments for the treatment of restenosis following percutaneous transluminal coronary angioplasty (PTCA) through magnetic stent hyperthermia (MSH). A 316L stainless steel stent and rabbit vascular smooth muscle cells (VSMCs) were used in the present study, in which the inductive heating characteristics of the stent under alternative magnetic field (AMF) exposure, as well as the effect of MSH on the proliferation, apoptosis, cell cycle and proliferating cell nuclear antigen (PCNA) expression of the rabbit VSMCs, were evaluated. The results demonstrated that 316L stainless steel coronary stents possess ideal inductive heating characteristics under 300 kHz AMF exposure. The heating properties were shown to be affected by the field intensity of the AMF, as well as the orientation the stent axis. MSH had a significant effect on the proliferation and apoptosis of VSMCs, and the effect was temperature-dependent. While a mild temperature of 43 degrees C demonstrated negligible effects on the growth of VSMCs, MSH treatment above 47 degrees C effectively inhibited the VSMC proliferation and induced apoptosis. Furthermore, a 47 degrees C treatment exhibited a significant and long-term inhibitory effect on VSMC migration. The results strongly suggested that MSH may be potentially applied in the clinic as an alternative approach for the prevention and treatment of restenosis.
作者机构：
[Xu, Jian] Post-Graduate School, Hunan University of Traditional Chinese Medicine, Changsha, Hunan 410208, China;[Peng, Qinghua] Department of Ophthalmology, the First Affiliated Hospital of Hunan University of Traditional Chinese Medicine, Changsha, Hunan 410007, China
通讯机构：
[Peng, Qinghua] Hunan Univ Tradit Chinese Med, Affiliated Hosp 1, Dept Ophthalmol, Changsha 410007, Hunan, Peoples R China.
摘要：
Current management of retinitis pigmentosa (RP) includes an attempt at slowing down the degenerative process through therapies that use either Western or traditional Chinese medicine (TCM). Novel therapies in Western medicine (WM) include use of tailor-made gene therapy, transplantation of stem cells, or neuroprotection treatment. TCM treatment includes two major approaches. These are orally applied herbal decoctions and acupuncture. In fact, all TCM treatments are based on the differentiation of a symptom-complex, which is the characteristic essence of TCM. Thus, diagnosed RP may be treated via the liver, the kidney, and the spleen. The principle behind these treatments is to invigorate the blood and brighten the eyes by toning up the liver and the kidney. Also treatments to cope with deficiencies in the two concepts that are unique and fundamental to TCM are considered: Qi or "vital energy" and Yin and Yang or the harmony of all the opposite elements and forces that make up existence. In particular, the Qi deficiency that results from blood stasis is addressed in these treatments. This paper also puts forward the existing problems and the prospect of the future development on integrating TCM with WM.
作者机构：
[Zhu, Junbao] Liuzhou Hospital of Traditional Chinese Medicine, Liuzhou 545001, Guangxi Zhuang Autonomous Region, China;[Zhang, Juan] Ningxiang Hospital of Traditional Chinese Medicine, Ningxiang 410600, Hunan Province, China;[Tian, Haomei; Zhang, Hong] Key Laboratory of Meridians and Viscera, Hunan University of Traditional Chinese Medicine, Changsha 410007, Hunan Province, China;[Cai, Hening; Zhang, Yuchen] Postgraduate College, Hunan University of Traditional Chinese Medicine, Changsha 410007, Hunan Province, China;[Chen, Chutao] Laboratory of Acupuncture Bioinformatics, Hunan University of Traditional Chinese Medicine, Changsha 410007, Hunan Province, China
通讯机构：
[Chen, Chutao] Hunan Univ Tradit Chinese Med, Lab Acupuncture Bioinformat, Changsha 410007, Hunan, Peoples R China.
摘要：
A middle cerebral artery occlusion-model was established in rats using the improved thread embolism method. Rats were treated with acupuncture at either Dazhui (DU14), Renzhong (DU26), Baihui (DU20), or a non-meridian point. Detection with protein-chip technology showed that the level of protein phosphorylation in both groups was upregulated or downregulated depending on the signaling pathway compared with the model group that did not receive acupuncture. Analysis of proteins showing downregulated phosphorylation revealed that five signaling pathways were activated in the acupuncture-treatment group, while only two were activated in the acupuncture- control group. In contrast, analysis of proteins showing upregulated phosphorylation revealed only one pathway was activated in the acupuncture-treatment group, whereas four pathways were activated in the acupuncture-control group. Furthermore, the number of activated proteins in the acupuncture-treatment group was not only higher than the acupuncture-control group, but unlike the acupuncture-control group, the majority of activated proteins were key proteins in the signaling pathways. Our findings indicate that acupuncture at specific points can activate multiple signaling pathways to promote the restoration of brain tissue following ischemic injury, and that this is based on a combination of effects resulting from multiple pathways, targets, and means.
作者机构：
[李莹; 潘诗敏; 李翠英; 张曦] the First Affiliated Hospital of Hunan University of CM, Changsha 410007, China;[李莹; 潘诗敏; 李翠英; 张曦] Graduate School of Hunan University of CM, Changsha 410208;[李金香] the First Affiliated Hospital of Hunan University of CM, Changsha 410007, China. 1306550930@qq.com
通讯机构：
[Li, Jinxiang] the First Affiliated Hospital of Hunan University of CM, Changsha 410007, China.
摘要：
Objective To explore the possible mechanism of Xinkang Granule in treating chronic heart failure（ CHF）. Methods Ninety Sprague Dawley（ SD） rats were randomized into a normal group（ n = 10）,a model group（ n = 16）,a control group（ n = 16）,and Xinkang low,medium and high dose groups（ n = 16 in each）. Rats in all groups were made CHF models by adriamycin vial 1. 5 mg/kg intraperitoneal injection except those in the normal group. The Xinkang low,medium and high dose groups were given 0. 5,1 and 2 g/（ kg·d） of Xinkang Granule,respectively. The control group was given 0. 06 g/（ kg·d） of Qili Qiangxin Capsule（ 芪苈强心胶囊）. The normal group and the model group were given equivalent normal saline by gavage. All groups were treated once every day for 8 weeks. Heart function of rats was examined. Cardiomyocyte apoptosis was examined by Td T-mediated d UTP nick end labeling（ TUNEL） stain. Myocardial fibrosis was examined by Masson stain. The expression levels of myocardium caspase 12（ Caspase-12）,glucose regulated protein 78（ GRP78）,CCAAT/enhancer-binding protein homologous protein（ CHOP） and mRNA were detected by methods of real-time polymerase chain reaction（ PCR） and immunohistochemistry. Results Compared with those in the model group,cardiomyocyte apoptosis and fibrosis in the Xinkang low,medium and high dose groups decreased. Compared with those in the model group,left ventricular diastolic diameter（ LVIDd）,left ventricular end systolic diameter（ LVESD）,Caspase-12,GRP78 and CHOP protein decreased in the control group,as well as the Xinkang low and medium dose groups,Caspase-12,GRP78 and CHOP mRNA decreased in the Xinkang medium dose group,but left ventricule ejection fraction（ LVEF）,fractional shortening of the left ventricular minor semi axis（ LVFS） and cardiac output（ CO） increased in the Xinkang medium dose group（ P 0. 05）. Compared with those in the control group,LVIDd and LVESD decreased but LVEF and CO increased in the Xinkang
