关键词：
Adult-onset type II citrullinemia (CTLN2);Aspartate-glutamate carrier (AGC);Citrin;Malate-aspartate shuttle;Neonatal intrahepatic cholestatic hepatitis (NICCD);Retrotransposal insertion;SLC25A13
摘要：
Deficiency of citrin, liver-type mitochondrial aspartate-glutamate carrier, is an autosomal recessive disorder caused by mutations of the SLC25A13 gene on chromosome 7q21.3 and has two phenotypes: neonatal intrahepatic cholestatic hepatitis (NICCD) and adult-onset type II citrullinemia (CTLN2). So far, we have described 19 SLC25A13 mutations. Here, we report 13 novel SLC25A13 mutations (one insertion, two deletion, three splice site, two nonsense, and five missense) in patients with citrin deficiency from Japan, Israel, UK, and Czech Republic. Only R360X was detected in both Japanese and Caucasian. IVS16ins3kb identified in a Japanese CTLN2 family seems to be a retrotransposal insertion, as the inserted sequence (2,667-nt) showed an antisense strand of processed complementary DNA (cDNA) from a gene on chromosome 6 (C6orf68), and the repetitive sequence (17-nt) derived from SLC25A13 was found at both ends of the insert. All together, 30 different mutations found in 334 Japanese, 47 Chinese, 11 Korean, four Vietnamese and seven non-East Asian families have been summarized. In Japan, IVS16ins3kb was relatively frequent in 22 families, in addition to known mutations IVS11 + 1G > A, 851del4, IVS13 + 1G > A, and S225X in 189, 173, 48 and 30 families, respectively; 851del4 and IVS16ins3kb were found in all East Asian patients tested, suggesting that these mutations may have occurred very early in some area of East Asia.
摘要：
Background: QiShenYiQi Pills (R) (QSYQ) is a compound Chinese medicine used in China for alleviating cardiac function. The present study was designed to explore the effect and mechanism of QSYQ on ischemia-reperfusion (I/R)-induced disorders in myocardial structure and function, with particularly focusing on the regulation of energy metabolism. Methods: Sprague-Dawley rats, with or without QSYQ pretreatment, were subjected to 30 min occlusion of the left anterior descending coronary artery and followed by 90 min or 24 h reperfusion. Myocardial blood flow (MBF) and cardiac function were evaluated at baseline, immediately after ischemia and 30, 60, 90 min, and 24 h after reperfusion. Myocardial infarction, myocardial histology and ultrastructure were assessed. Double staining of alpha-cardiac actinin and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling was conducted to assess myocardial apoptosis. ATP, ADP and AMP content was determined by Enzyme-Linked Immunosorbent Assay, F-actin in myocardial cells determined by immunofluorescence microscopy and expression of ATP synthase alpha, ATP5D, and phosphorylated-Myosin Light Chain (P-MLC) determined by western blotting. Results: Pre-treatment with QSYQ protected against I/R-induced MBF decrease, myocardial infarction and apoptosis at 90 min and 24 h after reperfusion. Moreover, I/R 90 min caused an impairment on cardiac function, a decrease in the ratio of ADP/ATP and AMP/ATP, accompanying with reduction of ATP 5D expression and increase in the expression of P-MLC, meanwhile, myocardium to exhibit myocardial fiber rupture, interstitial edema, and mitochondria swelling, all of which were significantly ameliorated by pre-treatment with QSYQ. Conclusions: The results of the present study suggest an involvement of regulation of energy metabolism in the action of QSYQ to protect against myocardial I/R injury. (C) 2012 Elsevier Ireland Ltd. All rights reserved.
摘要：
Fractionation of the EtOH extract from the fruits of Broussonetia papyrifera led to the isolation of 15 phenolic compounds (1-15). Their structures were identified using spectroscopic methods. Among these compounds, 1 and 2 are new and 3-15 were isolated from this plant for the first time. Antioxidant activities of compounds 2-15 against H(2)O(2)-induced injury in SY5Y cells and 1,1-diphenyl-2-picrylhydrazyl radical scavenging activities were evaluated.
期刊：
Journal of natural products,2010年73(5):865-869 ISSN：0163-3864
通讯作者：
Li, Y
作者机构：
[Zhou, Xiao-Jiang] Hunan Univ Tradit Chinese Med, Coll Pharm, Changsha 410007, Hunan, Peoples R China.;[Su, Jia; Cheng, Yong-Xian; Zhu, Hua-Jie; Zhao, Jun; Li, Yan; Wang, Yuan-Yuan] Chinese Acad Sci, State Key Lab Phytochem & Plant Resources W China, Kunming Inst Bot, Kunming 650204, Peoples R China.;[Zhao, Jun] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China.;[Yang, Tong-Hua] First Peoples Hosp Yunnan Prov, Dept Hematol, Kunming 650032, Peoples R China.
通讯机构：
[Li, Y] Chinese Acad Sci, State Key Lab Phytochem & Plant Resources W China, Kunming Inst Bot, Kunming 650204, Peoples R China.
摘要：
Three new isopimarane diterpenoids, trogopteroids A-C (1-3), our new aromatic diterpenoids, trogopteroids D-G (4-7), and 12 known diterpenoids were isolated from the feces of Trogopterus xanthipes. Their structures were identified using spectroscopic methods. The relative configuration of 1 was confirmed by quantum calculations. Compound 1 represents the first example of a norisopimarane diterpenoid with an 8 alpha,15 alpha-olide ring. With the exception of compound 14, all diterpenoids were evaluated for cytotoxicity against seven human tumor cell lines.
期刊：
MOLECULAR MEDICINE REPORTS,2015年12(3):4273-4283 ISSN：1791-2997
通讯作者：
Tang, N
作者机构：
[Wu, Lin; Li, Hai-Yuan; Wang, Qing-Bi; Cai, Xin-Kun; Tang, Nong; Bi, Xin-Ya; Li, Tian-Wei] Guangxi Univ Chinese Med, Guangxi Sci Expt Ctr Tradit Chinese Med, Nanning 530001, Guangxi, Peoples R China.;[Wu, Lin; Tang, Nong] Guangxi Univ Chinese Med, Guangxi Key Lab Chinese Med Fdn Res, Nanning 530001, Guangxi, Peoples R China.;[Zhao, Qing-Shan] Hunan Univ Chinese Med, Grad Sch, Changsha 410208, Hunan, Peoples R China.;[Tang, Nong] Guangxi Univ Chinese Med, Dept Internal Neurol, Affiliated Hosp 1, Nanning 530023, Guangxi, Peoples R China.
通讯机构：
[Tang, N] Guangxi Univ Chinese Med, Guangxi Sci Expt Ctr Tradit Chinese Med, 179 East Mingxiu Rd, Nanning 530001, Guangxi, Peoples R China.
关键词：
Chinese medicine;vascular dementia;memory impairment;ischemia/reperfusion;apoptosis;signal transduction
摘要：
Apoptosis and the dysfunction of the cyclic adenosine monophosphate (cAMP)/protein kinase A (PKA)/cAMP-responsive element binding protein (CREB) signaling pathway have a key role in memory impairment in vascular dementia (VaD), a challenging clinical problem. Yifei Xuanfei Jiangzhuo formula (YXJF), a Chinese herbal decoction, has been used to treat VaD in clinical practice and has produced positive outcomes; however, convincing evidence is currently lacking. The present study aimed to investigate the effects of YXJF on memory impairment in rats with cerebral ischemia/reperfusion and to explore the underlying mechanism. YXJF ameliorated memory impairment in rats with cerebral ischemia/reperfusion, inhibited hippocampal apoptosis in a dose-dependent manner and attenuated increases in the protein expression of B-cell lymphoma 2 (Bcl-2)-associated X protein as well as c-Jun and a reduction in Bcl-2 protein expression in the hippocampal tissue of the rats. Furthermore, administration of YXJF significantly increased the protein expression of PKA C-alpha and CREB, and promoted CREB phosphorylation. The results indicated that YXJF improves memory impairment through inhibiting apoptosis and enhancing PKA/CREB signal transduction in rats with cerebral ischemia/reperfusion.
期刊：
Frontiers of Medicine,2015年9(1):20-29 ISSN：2095-0217
通讯作者：
Hu, GH
作者机构：
[Hu, Guoheng] Department of Neurology, The First Affiliated Hospital of Hunan University of Traditional Chinese Medicine;[Li, Yingchen; Cheng, Qilai] Post-Graduate School, Hunan University of Traditional Chinese Medicine
通讯机构：
[Hu, GH] Hunan Univ Tradit Chinese Med, Dept Neurol, Affiliated Hosp 1, Changsha 410007, Hunan, Peoples R China.
关键词：
human umbilical cord;mesenchymal stem cells;ischemic stroke;cellular therapy;transplantation
摘要：
Ischemic stroke is a focal cerebral insult that often leads to many adverse neurological complications severely affecting the quality of life. The prevalence of stroke is increasing throughout the world, while the efficacy of current pharmacological therapies remains unclear. As a neuroregenerative therapy, the implantation of human umbilical cord mesenchymal stem cells (hUC-MSCs) has shown great possibility to restore function after stroke. This review article provides an update role of hUC-MSCs implantation in the treatment of ischemic stroke. With the unique “immunosuppressive and immunoprivilege” property, hUC-MSCs are advised to be an important candidate for allogeneic cell treatment. Nevertheless, most of the treatments are still at primary stage and not clinically feasible at the current time. Several uncertain problems, such as culture conditions, allograft rejection, and potential tumorigenicity, are the choke points in this cellular therapy. More preclinical researches and clinical studies are needed before hUC-MSCs implantation can be used as a routinely applied clinical therapy.
摘要：
Vascular dementia (VD) has been one of the most serious public health problems worldwide. It is well known that cerebral hypoperfusion is the key pathophysiological basis of VD, but it remains unclear how global genes in hippocampus respond to cerebral ischemia-reperfusion. In this study, we aimed to reveal the global gene expression profile in the hippocannpus of VD using a rat model. VD was induced by repeated occlusion of common carotid arteries followed by reperfusion. The rats with VD were characterized by deficit of memory and cognitive function and by the histopathological changes in the hippocampus, such as a reduction in the number and the size of neurons accompanied by an increase in intercellular space. Microarray analysis of global genes displayed up-regulation of 7 probesets with genes with fold change more than 1.5 (P < 0.05) and down-regulation of 13 probesets with genes with fold change less than 0.667 (P < 0.05) in the hippocampus. Gene Ontology (GO) and pathway analysis showed that the up-regulated genes are mainly involved in oxygen binding and transport, autoimmune response and inflammation, and that the down-regulated genes are related to glucose metabolism, autoimmune response and inflammation, and other biological process, related to memory and cognitive function. Thus, the abnormally expressed genes are closely related to oxygen transport, glucose metabolism, and autoimmune response. The current findings display global gene expression profile of the hippocampus in a rat model of VD, providing new insights into the molecular pathogenesis of VD.
期刊：
Progress in neuro-psychopharmacology & biological psychiatry,2016年75:183-191 ISSN：0278-5846
通讯作者：
Zhang, DS;Chen, NH
作者机构：
[Zuo, Wei; Du, Guo-Hua; Tian, Man-Tong; Chen, Jiao; Zhou, Heng; Chen, Nai-Hong; Lou, Yu-Xia; Wang, Zhen-Zhen] State Key Laboratory of Bioactive Substances and Functions of Natural Medicines, Institute of Materia Medica & Neuroscience Center, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, PR China;[Jin, Can; Zhang, Dan-Shen] Department of Pharmacology, Hebei Medical University, Shijiazhuang, Hebei 050017, PR China;[Wang, Zhi-Qi] College of Pharmacy, Hunan University of Chinese Medicine, Changsha, Hunan 410208, PR China;[Zhang, Dan-Shen] Hebei University of Science and Technology, Shijiazhuang, Hebei 050018, PR China. Electronic address: zhangds2011@126.com;[Yamakuni, Tohru; Kawahata, Ichiro] Department of Pharmacology, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai 980-8578, Japan
通讯机构：
[Zhang, DS] Hebei Univ Sci & Technol, Shijiazhuang 050018, Hebei, Peoples R China.;[Chen, NH] Peking Union Med Coll, Beijing 100050, Peoples R China.;Hebei Med Univ, Dept Pharmacol, Shijiazhuang 050017, Hebei, Peoples R China.;Chinese Acad Med Sci, Inst Mat Med & Neurosci Ctr, State Key Lab Bioact Subst & Funct Nat Med, Beijing 100050, Peoples R China.
摘要：
Ginsenoside Rg1 (Rg1) exhibits antidepressant-like activity by increasing neurogenesis and dendritic spine density without discernible side effects. However, the molecular mechanisms underlying Rg1 antidepressant activity remain poorly understood. As the dysfunction of gap junctions between astrocytes in the prefrontal cortex (PFC) is implicated in major depression disorder, the aim of this study was to investigate the effects of Rg1 on astrocyte gap junctions in the PFC. Rats exposed to chronic unpredictable stress (CUS) were administered Rg1 (5,10, and 20 mg/kg) for 28 days and analyzed for depressive symptoms using the sucrose preference and forced swimming tests. Functional and morphological changes of gap junction channels in the PFC were evaluated using dye transfer and electron microscopy, respectively. The expression of connexin 43 (Cx43) was analyzed by western blotting. Rg1 markedly alleviated depression-like behavior in rats. Long-term Rg1 treatment of CUS-exposed rats also significantly prevented the decrease in dye diffusion and improved the ultrastructure of astrocyte gap junctions in the PFC, indicating beneficial effects on the functional activity of gap junction channels in the brain. In addition, Rg1 upregulated.Cx43 expression in the PFC reduced by CUS exposure, which significantly correlated with its antidepressant-like effects. The results demonstrate that Rg1-induced antidepressant effects are might be mediated, in part, by protecting astrocyte gap junctions within the prefrontal cortex.(C) 2016 Elsevier Inc. All rights reserved.
作者机构：
[Lin, Hou-Wen] Research Center for Marine Drugs, Department of Pharmacy, State Key Laboratory of Oncogenes and Related Genes, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China. franklin67@126.com;[Han, Bing-Nan] Research Center for Marine Drugs, Department of Pharmacy, State Key Laboratory of Oncogenes and Related Genes, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China. hanbingnan@shsmu.edu.cn;[Yang, Zhen] Department of Pharmacy, Graduate School, Hunan University of Chinese Medicine, Changsha 410208, China. zyyangzhen1991@163.com;[Chai, Qiu-Ye] Research Center for Marine Drugs, Department of Pharmacy, State Key Laboratory of Oncogenes and Related Genes, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China. chaiqiuye123@sina.cn;[Chai, Qiu-Ye] School of Pharmacy, Jiangxi University of Traditional Chinese Medicine, Nanchang 330000, China. chaiqiuye123@sina.cn
通讯机构：
[Chai, Qiu-Ye; Han, Bing-Nan; Lin, Hou-Wen] Research Center for Marine Drugs, Department of Pharmacy, State Key Laboratory of Oncogenes and Related Genes, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China.;[Chai, Qiu-Ye] School of Pharmacy, Jiangxi University of Traditional Chinese Medicine, Nanchang 330000, China.;[Yang, Zhen] Department of Pharmacy, Graduate School, Hunan University of Chinese Medicine, Changsha 410208, China.
摘要：
Since the 1990s, a number of terminal alkynyl residue-containing cyclic/acyclic peptides have been identified from marine organisms, especially cyanobacteria and marine mollusks. This review has presented 66 peptides, which covers over 90% marine peptides with terminal alkynyl fatty acyl units. In fact, more than 90% of these peptides described in the literature are of cyanobacterial origin. Interestingly, all the linear peptides featured with terminal alkyne were solely discovered from marine cyanobacteria. The objective of this article is to provide an overview on the types, structural characterization of these unusual terminal alkynyl fatty acyl units, as well as the sources and biological functions of their composed peptides. Many of these peptides have a variety of biological activities, including antitumor, antibacterial, antimalarial, etc. Further, we have also discussed the evident biosynthetic origin responsible for formation of terminal alkynes of natural PKS (polyketide synthase)/NRPS (nonribosome peptide synthetase) hybrids.
期刊：
EXPERIMENTAL AND THERAPEUTIC MEDICINE,2013年6(2):347-354 ISSN：1792-0981
通讯作者：
Tang, JT
作者机构：
[Wang, Rui; Zhao, Ling-Yun; Li, Jing-Ding-Sha; Shi, Huan-Huan; Xie, Le; Kong, Wei-Chao; Tang, Jin-Tian; Li, Li] Tsinghua Univ, Inst Med Phys & Engn, Key Lab Particle & Radiat Imaging, Minist Educ,Dept Engn Phys, Beijing 100084, Peoples R China.;[Wang, Rui] Harbin Stomatol Hosp, Harbin 150010, Heilongjiang, Peoples R China.;[Li, Jing-Ding-Sha; Shi, Huan-Huan; Kong, Wei-Chao] Beijing Univ Chinese Med, Dept Biopharmaceut, Beijing 100102, Peoples R China.;[Shi, Huan-Huan; Ke, Da-Nian] Beijing MED Zenith Med Sci Co Ltd, Beijing 101312, Peoples R China.;[Xie, Le] Hunan Univ Chinese Med, Grad Sch, Changsha 410208, Hunan, Peoples R China.
通讯机构：
[Tang, JT] Tsinghua Univ, Dept Engn Phys, Room B116,Bldg Med Sci, Beijing 100084, Peoples R China.
关键词：
magnetic stent hyperthermia;percutaneous transluminal coronary angioplasty;restenosis;vascular smooth muscle cells;alternative magnetic field
摘要：
Thermal treatment or hyperthermia has received considerable attention in recent years due to its high efficiency, safety and relatively few side-effects. In this study, we investigated whether it was possible to utilize targeted thermal or in-stent thermal treatments for the treatment of restenosis following percutaneous transluminal coronary angioplasty (PTCA) through magnetic stent hyperthermia (MSH). A 316L stainless steel stent and rabbit vascular smooth muscle cells (VSMCs) were used in the present study, in which the inductive heating characteristics of the stent under alternative magnetic field (AMF) exposure, as well as the effect of MSH on the proliferation, apoptosis, cell cycle and proliferating cell nuclear antigen (PCNA) expression of the rabbit VSMCs, were evaluated. The results demonstrated that 316L stainless steel coronary stents possess ideal inductive heating characteristics under 300 kHz AMF exposure. The heating properties were shown to be affected by the field intensity of the AMF, as well as the orientation the stent axis. MSH had a significant effect on the proliferation and apoptosis of VSMCs, and the effect was temperature-dependent. While a mild temperature of 43 degrees C demonstrated negligible effects on the growth of VSMCs, MSH treatment above 47 degrees C effectively inhibited the VSMC proliferation and induced apoptosis. Furthermore, a 47 degrees C treatment exhibited a significant and long-term inhibitory effect on VSMC migration. The results strongly suggested that MSH may be potentially applied in the clinic as an alternative approach for the prevention and treatment of restenosis.
作者机构：
[Xu, Jian] Hunan Univ Tradit Chinese Med, Postgrad Sch, Changsha 410208, Hunan, Peoples R China.;[Peng, Qinghua] Hunan Univ Tradit Chinese Med, Affiliated Hosp 1, Dept Ophthalmol, Changsha 410007, Hunan, Peoples R China.
通讯机构：
[Peng, QH] Hunan Univ Tradit Chinese Med, Affiliated Hosp 1, Dept Ophthalmol, Changsha 410007, Hunan, Peoples R China.
摘要：
Current management of retinitis pigmentosa (RP) includes an attempt at slowing down the degenerative process through therapies that use either Western or traditional Chinese medicine (TCM). Novel therapies in Western medicine (WM) include use of tailor-made gene therapy, transplantation of stem cells, or neuroprotection treatment. TCM treatment includes two major approaches. These are orally applied herbal decoctions and acupuncture. In fact, all TCM treatments are based on the differentiation of a symptom-complex, which is the characteristic essence of TCM. Thus, diagnosed RP may be treated via the liver, the kidney, and the spleen. The principle behind these treatments is to invigorate the blood and brighten the eyes by toning up the liver and the kidney. Also treatments to cope with deficiencies in the two concepts that are unique and fundamental to TCM are considered: Qi or "vital energy" and Yin and Yang or the harmony of all the opposite elements and forces that make up existence. In particular, the Qi deficiency that results from blood stasis is addressed in these treatments. This paper also puts forward the existing problems and the prospect of the future development on integrating TCM with WM.
摘要：
A middle cerebral artery occlusion-model was established in rats using the improved thread embolism method.Rats were treated with acupuncture at either Dazhui(DU14),Renzhong(DU26), Baihui(DU20),or a non-meridian point.Detection with protein-chip technology showed that the level of protein phosphorylation in both groups was upregulated or downregulated depending on the signaling pathway compared with the model group that did not receive acupuncture.Analysis of proteins showing downregulated phosphorylation revealed that five signaling pathways were activated in the acupuncture-treatment group,while only two were activated in the acupuncture-control group.In contrast,analysis of proteins showing upregulated phosphorylation revealed only one pathway was activated in the acupuncture-treatment group,whereas four pathways were activated in the acupuncture-control group.Furthermore,the number of activated proteins in the acupuncture-treatment group was not only higher than the acupuncture-control group,but unlike the acupuncture-control group,the majority of activated proteins were key proteins in the signaling pathways.Our findings indicate that acupuncture at specific points can activate multiple signaling pathways to promote the restoration of brain tissue following ischemic injury,and that this is based on a combination of effects resulting from multiple pathways,targets,and means.
