期刊：
International Journal of Biological Macromolecules,2019年122:1115-1119 ISSN：0141-8130
通讯作者：
Huang, LQ;Huang, JH
作者机构：
[Xie, Hua-lin; Zhang, Shui-han; Huang, Jian-hua; Xie, Yi] Hunan Acad Chinese Med, Changsha 410013, Hunan, Peoples R China.;[Zhou, Rong-rong] Changchun Univ Chinese Med, Sch Pharm, Changchun 130117, Jilin, Peoples R China.;[Zhao, Chen-xi] Changsha Univ, Coll Biol & Environm Engn, Changsha 410022, Hunan, Peoples R China.;[Yu, Yi] Infinitus China Co Ltd, Guangzhou 510663, Guangdong, Peoples R China.;[Huang, LQ; Huang, Lu-qi; Zhou, Rong-rong] China Acad Chinese Med Sci, Natl Resource Ctr Chinese Mat Med, State Key Lab Breeding Base Daodi Herbs, Beijing 100700, Peoples R China.
通讯机构：
[Huang, LQ ; Huang, JH ] C;China Acad Chinese Med Sci, Natl Resource Ctr Chinese Mat Med, State Key Lab Breeding Base Daodi Herbs, Beijing 100700, Peoples R China.;China Acad Chinese Med Sci, Natl Resource Ctr Chinese Mat Med, Beijing, Peoples R China.
关键词：
Calibration model;Chemometrics;Geographical regions distinguish;Near-infrared spectroscopy;Polysaccharide contents
摘要：
In this study, a calibration model based on Near-infrared spectroscopy (NIR) technique and chemometrics method was developed for rapid and non-destructive detecting the polysaccharide contents of lentinula edodes samples collected from different regions. The polysaccharide contents of these samples were firstly determined by standard phenol-sulphruic acid method. Then, NIR spectra of these samples were collected by using Fourier transform infrared spectrometry. Based on these experimental data, a random forest method was further used to distinguish the regions of these samples, with a classification accuracy of 96.6%. After that, a rapid, accurate, and quantitative model was established for predicting the polysaccharide contents of these samples. In the model establishing process, some signal pre-treatment methods were optimized, and the validation results with highest determination coefficient (R-2) and low root mean square errors of prediction (RMSEP) were, 0.925 and 0.720, respectively. These results showed that combined NIR technique with chemometrics was an effective and green method for lentinula edodes quality control. (C) 2018 Elsevier B.V. All rights reserved.
作者机构：
[Zhou, H; Luo, Pei; Xie, Ying; Lio, Chon-Kit; Luo, Jin-Fang; Yao, Yun-Da; Zhou, Hua] Macau Univ Sci & Technol, Fac Chinese Med, Taipa 999078, Macao, Peoples R China.;[Zhou, H; Luo, Pei; Xie, Ying; Lio, Chon-Kit; Luo, Jin-Fang; Yao, Yun-Da; Zhou, Hua] Macau Univ Sci & Technol, State Key Lab Qual Res Chinese Med, Taipa 999078, Macao, Peoples R China.;[Yao, Xin-Sheng; Shen, Xiu-Yu] Shenyang Pharmaceut Univ, Coll Tradit Chinese Mat Med, Shenyang 110016, Liaoning, Peoples R China.;[Machado, Jorge] Univ Porto, Abel Salazar Inst Biomed Sci, ICBAS, Lab Appl Physiol, Rua Jorge Viterbo Ferreira 228, P-4050313 Porto, Portugal.;[Yao, Xin-Sheng; Yu, Yang; Dai, Yi] Jinan Univ, Coll Pharm, Inst Tradit Chinese Med & Nat Prod, Guangzhou 510632, Guangdong, Peoples R China.
通讯机构：
[Zhou, H ] M;[Liu, ZQ] G;Macau Univ Sci & Technol, Fac Chinese Med, Taipa 999078, Macao, Peoples R China.;Macau Univ Sci & Technol, State Key Lab Qual Res Chinese Med, Taipa 999078, Macao, Peoples R China.;Guangzhou Univ Chinese Med, Joint Lab Translat Canc Res Chinese Med, Minist Educ Peoples Republ China, Guangzhou 510006, Guangdong, Peoples R China.
