Sceletorines A and B, two minor novel dimeric alkaloids of Mesembryanthemum tortuosum (synonym Sceletium tortuosum)
作者：
Yin, Hongquan;Ali, Zulfiqar* ;Ding, Yuanqing;Wang, Yan-Hong;Cunningham, Michael J.;...
期刊：
Phytochemistry Letters ,2019年31:78-84 ISSN：1874-3900
通讯作者：
Ali, Zulfiqar;Khan, IA
作者机构：
[Ding, Yuanqing; Ali, Zulfiqar; Ibrahim, Mohamed A.; Chittiboyina, Amar G.; Yin, Hongquan; Cunningham, Michael J.; Khan, Ikhlas A.; Wang, Yan-Hong] Univ Mississippi, Natl Ctr Nat Prod Res, Sch Pharm, Mississippi State, MS 38677 USA.;[Yin, Hongquan] Beijing Inst Technol, Sch Chem Engn & Environm, Dept Appl Chem & Pharm, Beijing 100081, Peoples R China.;[Khan, Ikhlas A.] Univ Mississippi, Sch Pharm, Dept BioMol Sci, Div Pharmacognosy, Mississippi State, MS 38677 USA.;[Wang, Wei] Hunan Univ Chinese Med, Sch Pharm, Dev Lab, TCM & Ethnomed Innovat, Changsha 410208, Hunan, Peoples R China.;[Viljoen, Alvaro M.] Tshwane Univ Technol, Dept Pharmaceut Sci, Private Bag X680, ZA-0001 Pretoria, South Africa.
通讯机构：
[Ali, Z; Khan, IA ] U;Univ Mississippi, Natl Ctr Nat Prod Res, Sch Pharm, Mississippi State, MS 38677 USA.
关键词：
Mesembryanthemum tortuosum;Sceletium tortuosum;Sceletorine;Alkaloids;Absolute configuration;Structure elucidation;NMR
摘要：
Sceletorines A and B, two unique alkaloids, were isolated for the first time from the aerial parts of Mesembryanthemum tortuosum. Their structures were determined by the extensive spectroscopic methods and their absolute configuration was established with ECD calculations. Plausible biosynthetic pathways for 1 and 2 are proposed and a relationship to the previously assumed artifact, channaine is also addressed.
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不同灸量隔姜灸对脾虚证大鼠胃组织丝裂原细胞外激酶和细胞外调节蛋白激酶表达的影响
作者：
贲定严;宁炯杰;徐寅;罗燕;李木清;...
期刊：
中国中医药信息杂志 ,2018年25(1):54-58 ISSN：1005-5304
作者机构：
湖南中医药大学, 湖南, 长沙, 410208;湖南中医药大学第一附属医院, 湖南, 长沙, 410007;湖南省中医药研究院, 湖南, 长沙, 410006;[贲定严; 宁炯杰; 李木清; 王云辉; 易展] 湖南中医药大学, 湖南, 长沙, 410208;[徐寅] 湖南中医药大学第一附属医院, 湖南, 长沙, 410007
关键词：
灸量;隔姜灸;脾虚证;胃黏膜增殖修复;丝裂原细胞外激酶;细胞外调节蛋白激酶;大鼠
摘要：
目的 观察不同灸量隔姜灸对脾虚证大鼠胃组织丝裂原细胞外激酶(MEK)1/2和细胞外调节蛋白激酶(ERK)1/2表达的影响,探讨隔姜灸治疗脾虚证的可能作用机制及量效特征.方法 将75只SD大鼠按随机数字表法分为空白对照组、模型组、隔姜灸3壮组、隔姜灸6壮组、隔姜灸9壮组,每组15只.200%大黄浓缩液4 ℃灌胃制作脾虚证大鼠模型.造模成功后,隔姜灸组选取"足三里""中脘"予不同灸量治疗,连续8 d.HE染色镜下观察大鼠胃组织病理学改变,免疫组化检测大鼠胃组织MEK1/2及ERK1/2的蛋白表达.结果 与空白对照组比较,模型组大鼠胃黏膜损伤明显,可见较大的破损、脱落;与模型组比较,隔姜灸3壮组大鼠胃黏膜表面有部分脱落、破损情况改善,隔姜灸6壮组和隔姜灸9壮组大鼠胃黏膜表面较完整、脱落及破损明显改善.与空白对照组比较,模型组胃组织MEK1/2及ERK1/2蛋白表达明显升高(P<0.01);与模型组比较,隔姜灸各组胃组织MEK1/2及ERK1/2蛋白明显表达升高(P<0.01);与隔姜灸3壮组比较,隔姜灸6壮组和隔姜灸9壮组胃组织MEK1/2及ERK1/2蛋白表达明显升高(P<0.01),但二者作用效果相当,差异无统计学意义(P>0.05).结论 隔姜灸通过提高胃组织MEK1/2及ERK1/2的蛋白表达,激活MEK/ERK信号转导通路,进而促进脾虚证大鼠胃黏膜的修复.
语种：
中文
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不同灸量隔姜灸对脾虚证大鼠三叶因子1、粘蛋白5AC及表皮生长因子受体的影响
作者：
贲定严;宁炯杰;徐寅;罗燕;李木清;...
期刊：
针灸推拿医学：英文版 ,2018年16(1):1-7 ISSN：1672-3597
通讯作者：
Zhan Yi
作者机构：
[贲定严; 宁炯杰; 李木清; 王云辉; 易展] Hunan University of Chinese Medicine, Changsha, 410208, China;[徐寅] The First Hospital of Hunan University of Chinese Medicine, Changsha, 410007, China;[罗燕] Hunan Academy of Chinese Medicine, Changsha, 410006, China
通讯机构：
[Yi, Z ] ;Hunan Univ Chinese Med, Changsha 410208, Hunan, Peoples R China.
关键词：
Moxibustion Therapy;Ginger-partitioned Moxibustion;Point;Zusanli (ST 36);Point;Zhongwan (CV 12);Research on Acupoints;Gastric Mucosal Damage;Spleen Deficiency Syndrome;Rats
摘要：
Objective: To observe the effects of different doses of ginger-partitioned moxibustion on serum trefoil factor 1 (TFF1) and mucin 5AC (MUC5AC) levels, as well as the expression of epidermal growth factor receptor (EGFR) in gastric mucosa of rats with spleen deficiency syndrome, therefore, to explore the possible mechanism and the dose-effect characteristics of ginger-partitioned moxibustion in spleen deficiency syndrome. Methods: Seventy-five SPF grade Sprague-Dawley (SD) rats were randomly divided into a blank control group (group A), a model group (group B), a 3 moxa-cone ginger-partitioned moxibustion group (group C1), a 6 moxa-cone ginger-partitioned moxibustion group (group C2) and a 9 moxa-cone ginger-partitioned moxibustion group (group C3) using random number table method, 15 rats in each group. Except group A, rats in the other groups received intragastric administration of 4 °C 200% concentrated Da Huang (Radix et Rhizoma Rhei) to prepare spleen deficiency syndrome model. After successful modeling, rats in group B received no treatment; rats in group C1, C2 and C3 were treated with 3, 6 and 9 moxa-cone ginger-partitioned moxibustion at Zusanli (ST 36) and Zhongwan (CV 12) respectively for 8 continuous days. The general symptom score of rats was observed. The serum levels of TFF1 and MUC5AC were detected by enzyme-linked immunosorbent assay (ELISA). The expression of EGFR protein in gastric mucosa was detected by immunohistochemistry. Results: After the treatment, compared with group A, the spleen deficiency symptom score was increased in group B, the levels of serum TFF1 and MUC5AC, the EGFR protein expression in gastric tissues of group C1, C2 and C3 were significantly increased (all P<0.01); compared with group B, the spleen deficiency scores were decreased in group C1, C2 and C3, and the serum levels of TFF1 and MUC5AC, as well as EGFR protein expression in gastric tissues were increased (all P<0.01). Compared with group C1, the spleen deficiency scores were decreased in group C2 and C3, the serum levels of TFF1 and MUC5AC, and the expression of EGFR protein in gastric tissues were increased (all P<0.01), however, there was no significant difference between group C2 and C3 (all P>0.05). The mechanism may be related to the increase of serum TFF1 and MUC5AC levels and activation of EGFR protein. Conclusion: Ginger-partitioned moxibustion can improve the symptoms, as well as promote the proliferation and repair of gastric mucosa in rats with spleen deficiency. The therapeutic efficacy of 6 or 9 moxa-cone ginger-partitioned moxibustion is better than that of 3 moxa-cone ginger-partitioned moxibustion, while the efficacies are equivalent between 6 and 9 moxa-cone ginger-partitioned moxibustion groups. © 2018, Shanghai Research Institute of Acupuncture and Meridian.
语种：
英文
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7α-Hydroxyfriedelan-3-one-26-ol-29-oic acid and other Constituents from Pileostegia viburnoides var. glabrescens
作者：
Chen, Sheng-Huang;Yu, Juan;Li, Qin-Wen;Zhao, Jian-Ping;Wedge, David E.;...
期刊：
Natural Product Communications ,2016年11(7):931-934 ISSN：1934-578X
通讯作者：
Wang, W
作者机构：
[Chen, Sheng-Huang; Wang, Wei; Yu, Juan; Liao, Duan-Fang; Li, Qin-Wen; Khan, Ikhlas A.] Hunan Univ Chinese Med, Sch Pharm, Sino Luxemburg TCM Res Ctr, TCM & Ethnomed Innovat & Dev Lab, Changsha 410208, Hunan, Peoples R China.;[Zhao, Jian-Ping; Khan, Ikhlas A.] Univ Mississippi, Sch Pharm, Pharmaceut Sci Res Inst, Natl Ctr Nat Prod Res, University, MS 38677 USA.;[Wedge, David E.; Duke, Stephen O.] Univ Mississippi, ARS, USDA, Nat Prod Utilizat Res Unit,Natl Ctr Nat Prod Res, University, MS 38677 USA.;[Wang, Yu-Hong] Hunan Univ Chinese Med, State Key Lab Chinese Med Powder & Med Innovat Hu, Changsha 410208, Hunan, Peoples R China.;[Fronczek, Frank R.] Louisiana State Univ, Dept Chem, Baton Rouge, LA 70803 USA.
通讯机构：
[Wang, W ] H;Hunan Univ Chinese Med, Sch Pharm, Sino Luxemburg TCM Res Ctr, TCM & Ethnomed Innovat & Dev Lab, Changsha 410208, Hunan, Peoples R China.
关键词：
Pileostegia viburnoides var. glabrescens;Chemical constituents;Anti-inflammatory activity;Cytotoxicity;Phytotoxicity
摘要：
Phytochemical investigation of the roots and rhizomes of Pileostegia viburnoides var. glabrescens led to the isolation and identification of 31 compounds (1-31), 25 of which (1-2, 4-7, 9-12, 15-18, 21-31) were isolated from the Pileostegia genus for the first time. 7α-Hydroxyfriedelan-3-one-26-ol-29-oic acid (1) is a new friedelane-Type triterpene. The structure of n-butyl-β-D-fructopyranoside (2) was determined by single-crystal X-ray diffraction. Compounds 4 and 12 displayed marginal cytotoxicity against the P388 murine leukemia cell line with IC50 values of 13.4 μM and 25.0 μM, respectively. Compound 23 exhibited marginal anti-inflammatory activity by the inhibition of lipopolysaccharide induced nitric oxide production in RAW 264.7 macrophage cells, with an IC50 value of 32.0 μM. Compounds 3, 8-10, 25, and 27 were phytotoxic to the dicot Lactuca sativa (lettuce) and/or the monocot Agrostis stolonifera (bentgrass).
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英文
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Isoquinoline alkaloids from Zanthoxylum simulans and their biological evaluation
作者：
Yang, Sheng-Hui;Liu, Yan-Qun;Wang, Jun-Feng;Wang, Yue-Hu;Pan, Wei-Wei;...
期刊：
JOURNAL OF ANTIBIOTICS ,2014年68(4):289-292 ISSN：0021-8820
通讯作者：
Wang, JF
作者机构：
[Cai, Rui; Sheng, Wen-Bing; Yang, Sheng-Hui; Liu, Yan-Qun; Pan, Wei-Wei; Zhou, Xiao-Jiang] Hunan Univ Chinese Med, Coll Pharm, Changsha 410208, Hunan, Peoples R China.;[Wang, Jun-Feng; Wang, JF] Chinese Acad Sci, CAS Key Lab Trop Marine Bioresources & Ecol, Guangdong Key Lab Marine Mat Med, RNAM Ctr Marine Microbiol,South China Sea Inst Oc, Guangzhou 510301, Guangdong, Peoples R China.;[Wang, Yue-Hu] Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming, Peoples R China.
通讯机构：
[Wang, JF ] C;Chinese Acad Sci, CAS Key Lab Trop Marine Bioresources & Ecol, Guangdong Key Lab Marine Mat Med, RNAM Ctr Marine Microbiol,South China Sea Inst Oc, Guangzhou 510301, Guangdong, Peoples R China.
关键词：
6 acetonyl n methyldihydrodecarine;8 hydroxy 7 methoxy 5 methyl 2,3 meth ylenedioxybenzo[c]phenanthridin 6(5h) one;antibiotic agent;decarine;isoquinoline alkaloid;liriodenine;lysicamine;norchelerythrine;plant extract;unclassified drug;Zanthoxylum simulans extract;zanthoxylumine a;zanthoxylumine b;zanthoxylumine c;alkaloid;antirheumatic agent;isoquinoline derivative;antibacterial activity;Article;carbon nuclear magnetic resonance;drug screening;heteronuclear multiple bond correlation;high resolution electron ionization mass spectrometry;mass spectrometry;medicinal plant;nonhuman;priority journal;proton nuclear magnetic resonance;rheumatoid arthritis;Zanthoxylum;Zanthoxylum simulans;animal;Arthritis, Rheumatoid;chemistry;isolation and purification;metabolism;rat;spectroscopy;Zanthoxylum;Zanthoxylum simulans;Alkaloids;Animals;Antirheumatic Agents;Arthritis, Rheumatoid;Isoquinolines;Rats;Spectrum Analysis;Zanthoxylum
摘要：
[无可用摘要]
语种：
英文
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高校心理委员现状调查及对策分析
作者：
王云辉;刘微浪
期刊：
湖南第一师范学院学报 ,2009年9(4):39-40+53 ISSN：1674-831X
作者机构：
[刘微浪; 王云辉] 湖南中医药大学
关键词：
心理委员;心理健康;现状;对策
摘要：
目前高校心理委员工作由于相关政策、体制不完善,培训系统体系不健全,出现心理委员专业知识不足、作用发挥不充分、与院系和心理咨询室联系也不紧密的现象。大部分心理委员对自己的职责不太清楚,普遍缺乏心理学基本知识。高校应为心理委员的工作营造良好的工作氛围,健全心理委员的选拔、考核和激励机制,完善心理委员工作的运行机制,加强心理学知识的培训。
语种：
中文
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基于积极心理学理论的大学生社会适应能力培养
作者：
王云辉
期刊：
企业家天地(下半月刊) ,2009年(05):52-53 ISSN：1003-8434
作者机构：
湖南中医药大学
摘要：
随着大学生社会角色的变化,如何适应社会并为社会所容纳和接受,是摆在高校教育工作者面前的迫切问题。本文从积极心理学理论角度,对加强大学生社会适应能力的重要性、必要性进行分析,并从积极心理学与大学生社会适应的关系出发,对如何培养大学生社会适应性进行了探讨。
语种：
中文
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大学生人际交往心理特征及问题应对
作者：
王云辉
期刊：
湖南省社会主义学院学报 ,2008年(4):57-58+62 ISSN：1009-2293
作者机构：
[王云辉] 湖南中医药大学
关键词：
心理障碍;人际交往;大学生
摘要：
良好的人际交往能力以及良好的人际关系是人们生存和发展的必要条件。大学生作为一个特殊群体,有其特殊的人际关系构成体系,由于其特殊的心理在与周边特定的群体和个人进行交往时会产生一系列的人际交往问题。本文叙述了大学生人际交往的心理特征,阐述了由于人际交往不良给大学生的学习、生活、情绪、健康等各个方面带来了一系列不良的影响。说明了使学生建立良好的人际关系,形成一种团结友爱、朝气蓬勃的人际交往环境,将有利于大学生形成和发展健康的个性品质。
语种：
中文
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新时期高校辅导员应抓的“三个基本点”
作者：
王云辉
期刊：
科技信息(学术研究) ,2008年(21):634+636 ISSN：1001-9960
作者机构：
[王云辉] 湖南中医药大学
关键词：
辅导员;工作;方法
摘要：
在新的历史时期,不断变化和发展着的形势对高校辅导员工作提出了新的要求,认真修炼、增强自身素质、创新工作方法并抓住学生工作几个关键的问题是做好高校辅导员工作的三个基本环节。
语种：
中文
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