期刊：
BioTechnology: An Indian Journal,2013年8(4):543-550 ISSN：0974-7435
通讯作者：
Liao, D.-F.(dfliao66@aliyun.com)
作者机构：
[Tuo, Qin-Hui; Liao, Duan-Fang; Gong, Yong-Zhen; Sun, Shao-Wei] Hunan University of Chinese Medicine, Hanpu Science and Education District, 1# Xiangzui Road, Changsha 410208, Hunan, China;[Ke-Tang, Chao] Institute of Cardiovascular Disease, Life Science Research Center, University of South China, 28# Changsheng Western Road, Hengyang 421001, Hunan, China;[Tuo, Qin-Hui; Ke-Tang, Chao; Liao, Duan-Fang; Gong, Yong-Zhen; Zhang, Xiao-Ying; Sun, Shao-Wei] Division of biochemistry, School of pharmacy and Life Science, University of South China, 28# Changsheng West Road, Hengyang 421001, Hunan, China;[Zhang, Xiao-Ying] Division of Pharmacoproteomics, School of pharmacy and Life Science, University of South China, 28# Changsheng Western Road, Hengyang 421001, Hunan, China
通讯机构：
[Liao, D.-F.] H;Hunan University of Chinese Medicine, 1# Xiangzui Road, Changsha 410208, Hunan, China
期刊：
Journal of Molecular Neuroscience,2013年50(1):70-77 ISSN：0895-8696
通讯作者：
Tang, Xiao-Qing
作者机构：
[Ren, Yan-Kai; Chen, Rong-Qian; Tang, Xiao-Qing] Univ South China, Coll Med, Inst Cognit & Nervous Syst Dis, Hengyang 421001, Hunan, Peoples R China.;[Ren, Yan-Kai; Tang, Xiao-Qing] Univ South China, Coll Med, Dept Physiol, Hengyang 421001, Hunan, Peoples R China.;[Liao, Duan-Fang] Hunan Univ Chinese Med, Div Stem Cell Regulat & Applicat, State Key Lab Chinese Med Powder & Med Innovat Hu, Changsha 420108, Hunan, Peoples R China.;[Del Soldato, Piero] CTG Pharma, I-20131 Milan, Italy.;[Sparatore, Anna] Univ Milan, Dept Pharmaceut Sci Pietro Pratesi, Milan, Italy.
通讯机构：
[Tang, Xiao-Qing] U;Univ South China, Coll Med, Inst Cognit & Nervous Syst Dis, 28 W Changsheng Rd, Hengyang 421001, Hunan, Peoples R China.
关键词：
H2S-releasing sildenafil;Homocysteine;Neurotoxicity;Paraoxonase-1;Reactive oxygen species
摘要：
ACS6, a novel hydrogen sulfide (H2S)-releasing sildenafil, has been demonstrated to inhibit superoxide formation through donating H2S. We have previously found that ACS6 antagonizes homocysteine-induced apoptosis and cytotoxicity. The aim of the present study is to explore the molecular mechanisms underlying ACS6-exerted protective action against the neurotoxicity of homocysteine. In the present work, we used PC12 cells to explore whether paraoxonase-1 (PON-1) is implicated in ACS6-induced neuroprotection against homocysteine neurotoxicity. We show that ACS6 treatment results in prevention of homocysteine-caused neurotoxicity and overproduction of reactive oxygen species (ROS). Homocysteine downregulates the expression and activity of PON-1; however, this effect is significantly blocked by co-treatment with ACS6. The specific inhibitor of PON-1 2-hydroxyquinoline reverses the inhibitory effect of ACS6 on homocysteine-induced neurotoxicity and intracellular ROS accumulation. These results indicate that ACS6 protects PC12 cells against homocysteine-induced neurotoxicity by upregulating PON-1 and suggest a promising role of PON-1 as a novel therapeutic strategy for homocysteine-induced toxicity.
作者机构：
[Gu Hong-Feng] Univ South China, Dept Physiol, Hengyang 421001, Peoples R China.;[Gu Hong-Feng; Yang Yong-Zong] Key Lab Arteriosclerol Hunan Prov, Hengyang 421001, Peoples R China.;[Jiang Jian-Hong] Nanhua Univ, Chuanshan Coll, Hengyang 421001, Peoples R China.;[Tong Qiao-Zhen; Liao Duan-Fang] Hunan Univ Chinese Med, Div Stem Cell Regulat & Applicat, State Key Lab Chinese Med Powder & Med Innovat Hu, Changsha 410208, Hunan, Peoples R China.
通讯机构：
[Gu Hong-Feng] U;Univ South China, Dept Physiol, Hengyang 421001, Peoples R China.
关键词：
GP Ⅱb/Ⅲa;atherosclerosis;HMGB-1;TLR4;NF-κB
摘要：
The effects of glycoprotein (GP) Ⅱb/Ⅲa inhibitors on the development of the atherosclerotic process has received scant attention. To investigate whether GP Ⅱb/Ⅲa blockade influences atherosclerosis lesion and HMGB-1/TLR4 signaling, we compared plaque formation in ApoE~(-/-) mice: control group (n=10); IgG group (n=10, 50 μg) and GP Ⅱb/Ⅲa mAb group (n=10, 50 μg). All mice were fed on a Western diet (10% fat and 1.25% cholesterol) for 10 weeks. GP Ⅱb/Ⅲa blockade significantly decreased the atherosclerotic lesion and platelet adhesion to the vessel wall. Immunohistochemistry analysis showed that blocking GP Ⅱb/Ⅲa diminished MOMA-2 and VCAM-1 expression in aortic plaque in ApoE~(-/-) mice. Western blot results indicated that HMGB-1, TLR4, and NF-κB levels were markedly reduced in arteries of ApoE-/- mice treated with GP Ⅱb/Ⅲa mAb (P < 0.05). Moreover, GP Ⅱb/Ⅲa mAb decreased plasma HMGB-1, IL-1β, TNF-α and MCP-1 concentrations. Our findings demonstrated that GP Ⅱb/Ⅲa mAb significantly decreased atherosclerotic lesions and HMGB-1, TLR4 and NF-κB expression in ApoE~(-/-) mice (P < 0.05). The present study has suggested a possibility that GP Ⅱb/Ⅲa blockade attenuates atherosclerosis by inhibiting the HMGB-1/TLR4 pathway..
作者：
Song Yin;Xing Zheng;Xu Yao;Yuhong Wang;Duanfang Liao;...
期刊：
癌症治疗(英文),2013年4(1):113-123 ISSN：2151-1934
作者机构：
Division of Stem Cell Regulation and Application, State Key Laboratory of Chinese Medicine Powder and Medicine Innovation in Hunan (Incubation), Hunan University of Chinese Medicine, Changsha, China;Institute of Pharmacy and Pharmacology, University of South China, Hengyang, China
摘要：
Curcumin has been reported to possess multifunctional bioactivities with low toxicity. However, the clinical application of curcumin has been significantly limited by its instability and poor metabolic property. Up to now, multiple approaches are being sought to overcome these limitations to obtaining “super curcumin”, and many analogues of curcumin have been designed and synthesized. In all of those analogues, a series of mono-carbonyl curcumin analogues deleting the β-diketone draw our attention. Since the seven-carbon β-diketone linker in curcumin may be responsible for its instability, the series of mono-carbonyl curcumin analogues deleting the β-diketone may be potential prodrug with improved pharmacokinetic and pharmacodynamic properties. This review just focuses on these more stable mono-carbonyl analogues of curcumin, and shows the new class of active structure by introducing the synthesis and anticancer activity of them.
