摘要：
Abstract: Objective: Explain the intervention results of Danlong Xingnao Recipe on various indexes of rats with vascular dementia at multiple levels, and explore the mechanism of Danlong Xingnao recipe in the treatment of VD from the perspective of Notch signal pathway and its downstream target genes. Method: In 60 rats, the VD rat model was prepared by modified bilateral common carotid artery permanent occlusion (2-VO) and castration. The model rats were divided into four groups: Model group, positive drug group, DAPT group and Danlong Xingnao formula group. After the intervention measures were applied respectively, the morphological changes of hippocampus of rats stained with he were observed under light microscope, The levels of serum T, no and ET-1 were detected after administration. Count the number of double positive cells in immunofluorescence staining of proliferative cells in hippocampus, and the expression of Notch1 and Hes1 protein in hippocampal tissue of VD rats was detected by immunohistochemistry and Western blot. Results: After the intervention, the hippocampal tissue of each group showed certain changes occurred in the hippocampus of rats in each group. Compared with the model group, the levels of T and NO in the positive drug group and the Danlong Xingnao formula group increased significantly, and and the level of ET-1 decreased significantly (P < 0.01, P < 0.05); compared with the model group, the number of double positive cells in the hippocampus of rats in the positive drug group and the Danlong Xingnao formula group increased significantly (P < 0.01, P < 0.05); in sham operation group and DAPT group, a little expression of Notch1 and Hes1 protein can be seen in hippocampus, the expression of model group is enhanced, and the expression of Danlong Xingnao formula group and positive drug histone is significantly enhanced. Conclusion: Danlong Xingnao recipe can significantly improve the learning ability, memory ability, space exploration ability and brain pathological damage of VD rats; Danlong Xingnao recipe can promote the proliferation and differentiation of neural stem cells by actively regulating the expression of Notch signal pathway and its down-stream target gene Hes1 in VD rats, so as to play a therapeutic role in VD rats.#@#@#摘要: 目的：多层次地阐明丹龙醒脑方对血管性痴呆大鼠各指标的干预结果，从Notch信号通路及其下游靶基因角度探讨丹龙醒脑方治疗血管性痴呆(Vascular Dementia, VD)的作用机制。方法：60只大鼠通过改良双侧颈总动脉永久阻断法(2-VO)加去势手术法制备VD大鼠模型。将成模大鼠均分为四个大组，即模型组、阳性药物组、DAPT组、丹龙醒脑方组。分别运用干预措施后，光镜下观察HE染色的大鼠海马组织形态学变化，检测给药干预后大鼠血清T水平，及NO和ET-1水平。统计大鼠海马区、皮质区样本增殖活性细胞免疫荧光染色双阳性细胞数，使用免疫组织化学法、Western Blot法定性、定量检测VD大鼠海马组织Notch1、Hes1蛋白表达。结果：干预后各大组大鼠海马组织出现一定变化，与模型组比较，阳性药物组、丹龙醒脑方组T、NO水平均有明显上升，ET-1水平有明显下降(P < 0.01, P < 0.05)；与模型组比较，阳性药物组、丹龙醒脑方组大鼠海马区双阳性细胞数均有明显上升(P < 0.01, P < 0.05)；假手术组与DAPT组海马区可见Notch1、Hes1蛋白少许表达，模型组表达增强，丹龙醒脑方组、阳性药物组蛋白表达明显增强。结论：丹龙醒脑方能显著改善VD大鼠的学习能力、记忆能力、空间探索能力及脑组织病理损伤；丹龙醒脑方能通过积极调控VD大鼠Notch信号通路及其下游靶基因Hes1的表达，促进了神经干细胞的增殖、分化，从而发挥对VD大鼠的治疗作用。
期刊：
Computational and Mathematical Methods in Medicine,2022年2022:9043300 ISSN：1748-6718
通讯作者：
Zhu, M.
作者机构：
[Zhao, Yudong] Chengdu Univ Tradit Chinese Med, Sch Clin Med, Chengdu 610075, Peoples R China.;[Xia, Yu] Hunan Univ Chinese Med, Prov Key Lab TCM Diagnost, Changsha 410208, Peoples R China.;[Kuang, Gaoyan] Hunan Univ Chinese Med, Dept Orthopaed, Affiliated Hosp 1, Changsha 410007, Peoples R China.;[Cao, Jihui] Changshou Dist Hosp Tradit Chinese Med, Dept Orthopaed & Traumatol, Chongqing 400000, Peoples R China.;[Shen, Fu] Yong Zhou Hosp Tradit Chinese Med, Dept Orthopaed, Yongzhou 425000, Peoples R China.
通讯机构：
Department of Orthopaedics, Hospital of Chengdu University of Traditional Chinese Medicine, China
摘要：
<i>Background.</i> Knee osteoarthritis (KOA) is a common degenerative joint disease. In this study, we aimed to identify new biomarkers of KOA to improve the accuracy of diagnosis and treatment. <i>Methods.</i> GSE98918 and GSE51588 were downloaded from the Gene Expression Omnibus database as training sets, with a total of 74 samples. Gene differences were analyzed by Gene Ontology, Kyoto Encyclopedia of Genes and Genomes pathway, and Disease Ontology enrichment analyses for the differentially expressed genes (DEGs), and GSEA enrichment analysis was carried out for the training gene set. Through least absolute shrinkage and selection operator regression analysis, the support vector machine recursive feature elimination algorithm, and gene expression screening, the range of DEGs was further reduced. Immune infiltration analysis was carried out, and the prediction results of the combined biomarker logistic regression model were verified with GSE55457. <i>Results.</i> In total, 84 DEGs were identified through differential gene expression analysis. The five biomarkers that were screened further showed significant differences in cartilage, subchondral bone, and synovial tissue. The diagnostic accuracy of the model synthesized using five biomarkers through logistic regression was better than that of a single biomarker and significantly better than that of a single clinical trait. <i>Conclusions.</i> CX3CR1, SLC7A5, ARL4C, TLR7, and MTHFD2 might be used as novel biomarkers to improve the accuracy of KOA disease diagnosis, monitor disease progression, and improve the efficacy of clinical treatment.
作者：
Mao Yizhi;Li Liang;Luo Zhihong;Huang Yahui;Wu Huaying;...
期刊：
数字中医药(英文),2022年5(3):317-325 ISSN：2096-479X
通讯作者：
Peng Qinghua
作者机构：
Provincial Key Laboratory of TCM Diagnostics, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;Key Laboratory of TCM Heart and Lung Syndrome Differentiation&Medicated Diet and Dietotherapy, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;[Luo Zhihong; Yang Ping] Department of Psychiatry, Hunan Brain Hospital, Changsha, Hunan 410007, China;[Huang Yahui] Department of Neurosurgery, Hunan Brain Hospital, Changsha, Hunan 410007, China;[Wu Huaying] Medicine School, Hunan Normal University, Changsha, Hunan 410013, China
通讯机构：
[Peng Qinghua] P;Provincial Key Laboratory of TCM Diagnostics, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China<&wdkj&>Key Laboratory of TCM Heart and Lung Syndrome Differentiation&Medicated Diet and Dietotherapy, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China
摘要：
To explore the effect and mechanism of Chaihu Longgu Muli Decoction (²ñºúÁú¹ÇĵòÃÌÀ, CHLGMLD) in rats with temporal lobe epilepsy (TLE).
A total of 80 Sprague-Dawley (SD) male rats were randomized into control (CON), model (MOD), carbamazepine (CBZ, 0.1 g/kg), CHLGMLD low dose (CHLGMLD-L, 12.5 g/kg), and high dose (CHLGMLD-H, 25 g/kg) groups, with 16 rats in each group. TLE rat models were established in the four groups with the use of lithium-pilocarpine except for the CON group. After the successful establishment of TLE models, all drugs were administered through gavage, and distilled water was given to rats in the CON and MOD groups for four weeks. The frequency and duration of seizures before and after treatment were recorded for the evaluation of the alleviation degree. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the expression levels of miR-146a-3p and miR-146a-5p. The expression levels of toll-like receptor 4 (TLR4), interleukin-1 receptor-associated kinase 1 (IRAK1), tumor necrosis factor (TNF) receptor-associated factor 6 (TRAF6), TAK1-binding protein (TAB), nuclear factor-kappa B (NF-¦ÊB), and interleukin-1 beta (IL-1¦Â) in hippocampus were tested by immunofluorescence assay. Correlation analysis between the above factors and expressions of miR-146a-3p and miR-146a-5p were performed separately.
CHLGMLD decreased the frequency (P < 0.05) and duration (P < 0.01) of seizures in rats. CHLGMLD down-regulated the expression levels of miR-146a-5p and miR-146a-3p (P < 0.05), and inhibited the expression levels of TLR4, IRAK1, TRAF6, TAB, NF-¦ÊB, and IL-1¦Â (P < 0.01). The correlation analysis revealed that the expression levels of TLR4, IRAK1, TRAF6, TAB, NF-¦ÊB, and IL-1¦Â were positively correlated with the expression levels of miR-146a-3p and miR-146a-5p detected by qRT-PCR, respectively (P < 0.01).
CHLGMLD can inhibite the TLR4 signaling pathway by lowering the expression levels of miR-146a-3p and miR-146a-5p to alleviate hippocampal dentate gyrus inflammation in TLE rats, thus relieving seizures.
作者机构：
[方格; 钟森杰; 黄淑敏; 熊霞军; 杨梦; 胡志希; 李琳; 张倩; 王梓仪; 胡思远] Institute of Traditional Chinese Medicine Diagnosis, Hunan University of Chinese Medicine, Changsha, 410208, China
通讯机构：
[Hu, Z.-X.; Li, L.] I;Institute of Traditional Chinese Medicine Diagnosis, China
作者机构：
[魏林; 刘永芳; 陈帮明; 付义; 吴兆东] Jiangxi Provincial Clinical Medicine, Research Center of Chinese Medicine Nephropathy, Jiujiang Hospital of Chinese Medicine, Jiujiang, 332000, China;[李鑫] Hunan University of Chinese Medicine, Hunan Provincial Key Laboratory of Diagnostics of Chinese Medicine, Hunan Provincial Key Laboratory of Syndrome Differentiation of Cardio-Pulmonary Disease, Chinese Medicative Diet for Food Therapy, Changsha, 410208, China
通讯机构：
[Li, X.] H;Hunan University of Chinese Medicine, China
作者机构：
[李玲; 郑彩杏; 赖丽娜] Hunan University of Chinese Medicine, Changsha, 410208, China;Hunan Provincial Key Laboratory of Diagnostics of Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha, 410208, China;[李金霞; 周小青] Hunan University of Chinese Medicine, Changsha, 410208, China, Hunan Provincial Key Laboratory of Diagnostics of Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha, 410208, China
通讯机构：
[Zhou, X.] H;Hunan University of Chinese MedicineChina
作者机构：
[胡伊蕾; 赵彦禛; 周曼丽; 张宜帆; 俞赟丰] School of Traditional Chinese Medicine（TCM), Hunan University of Chinese Medicine, Changsha, 410208, China;Hunan Provincial Key Laboratory of Diagnostics of TCM, Hunan University of Chinese Medicine, Changsha, 410208, China;[简维雄] School of Traditional Chinese Medicine（TCM), Hunan University of Chinese Medicine, Changsha, 410208, China, Hunan Provincial Key Laboratory of Diagnostics of TCM, Hunan University of Chinese Medicine, Changsha, 410208, China
通讯机构：
[Jian, W.] S;School of Traditional Chinese Medicine（TCM), China
