摘要：
Respiratory diseases are an emerging public health concern, that pose a risk to the global community. There, it is essential to establish effective treatments to reduce the global burden of respiratory diseases. Astragaloside IV (AS-IV) is a natural saponin isolated from Radix astragali (Huangqi in Chinese) used for thousands of years in Chinese medicine. This compound has become increasingly popular due to its potential anti-inflammatory, antioxidant, and anticancer properties. In the last decade, accumulated evidence has indicated the AS-IV protective effect against respiratory diseases. This article presents a current understanding of AS-IV roles and mechanisms in combatting respiratory diseases. The ability of the agent to suppress oxidative stress, cell proliferation, and epithelial-mesenchymal transition (EMT), to attenuate inflammatory responses, and modulate programmed cell death (PCD) will be discussed. This review highlights the current challenges in respiratory diseases and recommendations to improve disease management.
作者机构：
[Wu, Zixuan; Song, Zhenyan; Cheng, Shaowu; Feng, Xiang; Zhu, Xu; Cheng, SW; Yang, Miao; Yu, Wenjing; Deng, Sisi] Hunan Univ Chinese Med, Coll Integrated Tradit Chinese & Western Med, Key Lab Hunan Prov Integrated Tradit Chinese & Wes, Changsha 410128, Peoples R China.
通讯机构：
[Cheng, SW ] H;Hunan Univ Chinese Med, Coll Integrated Tradit Chinese & Western Med, Key Lab Hunan Prov Integrated Tradit Chinese & Wes, Changsha 410128, Peoples R China.
摘要：
Autism spectrum disorder (ASD) is a neurodevelopmental disorder that usually manifests in childhood and is thought to be caused by a complex interaction of genetic, environmental, and immune factors. The majority of current ASD diagnostic methods rely on subjective behavioral observation and scale assessment, making early detection difficult. In this study, we confirmed that lysosomal-associated membrane protein 1 (LAMP1), a functional marker of immune cell activation and cytotoxic degranulation, was upregulated in ASD blood, brain cortex, and various genetic animal models or cells using bioinformatics approaches. The prognostic value of LAMP1 was investigated by correlating its expression with clinical ASD rating scales, and the receiver operating characteristic (ROC) curve analysis in ASD also revealed that it has a favorable diagnostic ability in distinguishing ASD from control cohort. According to gene set enrichment analysis (GSEA) results, LAMP1 correlated with genes that were enriched in natural kill and T cell immune function. Taking all of the evidence into account, we discovered that abnormal elevations of LAMP1 mRNA and protein in the blood of ASD children, may influence the development of ASD through its involvement in immune cell activity regulation. This report highlights a novel marker for ASD early detection as well as potential therapeutic targets.
期刊：
European Journal of Medical Research,2023年28(1):1-15 ISSN：2047-783X
通讯作者：
Changqing Deng
作者机构：
[Yahong Cai] Chronic Disease Management Department, The First Hospital of Hunan University of Chinese Medicine, Changsha, China;[Changqing Deng] The Key Laboratory of Hunan Province for Integrated Traditional Chinese and Western Medicine on Prevention and Treatment of Cardio-Cerebral Diseases, College of Integrated Traditional Chinese and Western Medicine, Hunan University of Chinese Medicine, Changsha, China;[Linquan Liu] Chronic Disease Management Department, The First Hospital of Hunan University of Chinese Medicine, Changsha, China<&wdkj&>The Key Laboratory of Hunan Province for Integrated Traditional Chinese and Western Medicine on Prevention and Treatment of Cardio-Cerebral Diseases, College of Integrated Traditional Chinese and Western Medicine, Hunan University of Chinese Medicine, Changsha, China
通讯机构：
[Changqing Deng] T;The Key Laboratory of Hunan Province for Integrated Traditional Chinese and Western Medicine on Prevention and Treatment of Cardio-Cerebral Diseases, College of Integrated Traditional Chinese and Western Medicine, Hunan University of Chinese Medicine, Changsha, China
摘要：
Pyroptosis plays an important role in the pathological process of ischemic stroke (IS). However, the exact mechanism of pyroptosis remains unclear. This paper aims to reveal the key molecular markers associated with pyroptosis in IS. We used random forest learning, gene set variation analysis, and Pearson correlation analysis to screen for biomarkers associated with pyroptosis in IS. Middle cerebral artery occlusion/reperfusion (MCAO/R) and oxygen and glucose deprivation/reoxygenation (OGD/R) models were constructed in vitro and in vivo. Cells were transfected with an Annexin A3 silencing (si-ANXA3) plasmid to observe the effects of ANXA3 on OGD/R + lipopolysaccharides (LPS)-induced pyroptosis. qRT‒PCR and western blotting were used to detect the expression of potential biomarkers and pyroptotic pathways. Samples from a total of 170 IS patients and 109 healthy individuals were obtained from 5 gene expression omnibus databases. Thirty important genes were analyzed by random forest learning from the differentially expressed genes. Then, we investigated the relationship between the above genes and the pyroptosis score, obtaining three potential biomarkers (ANXA3, ANKRD22, ADM). ANXA3 and ADM were upregulated in the MCAO/R model, and the fold difference in ANXA3 expression was greater. Pyroptosis-related factors (NLRP3, NLRC4, AIM2, GSDMD-N, caspase-8, pro-caspase-1, cleaved caspase-1, IL-1β, and IL-18) were upregulated in the MCAO/R model. Silencing ANXA3 alleviated the expression of pyroptosis-related factors (NLRC4, AIM2, GSDMD-N, caspase-8, pro-caspase-1, cleaved caspase-1, and IL-18) induced by OGD/R + LPS or MCAO/R. This study identified ANXA3 as a possible pyroptosis-related gene marker in IS through bioinformatics and experiments. ANXA3 could inhibit pyroptosis through the NLRC4/AIM2 axis.
作者机构：
[Zhang, Wei; Li, Jing; Yan, Fanchen] Hunan Univ Chinese Med, Coll Integrated Tradit Chinese & Western Med, Key Lab Hunan Prov Integrated Tradit Chinese & Wes, Changsha 410208, Hunan, Peoples R China.
通讯机构：
[Zhang, Wei] T;The Key Laboratory of Hunan Province for Integrated Traditional Chinese and Western Medicine on Prevention and Treatment of Cardio-Cerebral Diseases, College of Integrated Traditional Chinese and Western Medicine, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China. Electronic address:
期刊：
Journal of Inflammation Research,2023年16:4165-4211 ISSN：1178-7031
通讯作者：
Song, Zhenyan;Cheng, SW
作者机构：
[He, Chunxiang; Li, Ze; Song, Zhenyan; Cheng, Shaowu; Song, ZY; He, Jiawei; Cheng, SW; Chen, Qi; Yang, Miao; Luo, Rongsiqing; Zhou, Jinyong; Yu, Wenjing] Hunan Univ Chinese Med, Sch Integrated Chinese & Western Med, Changsha, Hunan, Peoples R China.;[He, Chunxiang; Li, Ze; Song, Zhenyan; Cheng, Shaowu; Song, ZY; He, Jiawei; Cheng, SW; Chen, Qi; Yang, Miao; Luo, Rongsiqing; Zhou, Jinyong; Yu, Wenjing] Hunan Univ Chinese Med, Coll Integrated Tradit Chinese & Western Med, Key Lab Hunan Prov Integrated Tradit Chinese & Wes, Changsha, Hunan, Peoples R China.
通讯机构：
[Song, ZY; Cheng, SW ] H;Hunan Univ Chinese Med, Sch Integrated Chinese & Western Med, Changsha, Hunan, Peoples R China.;Hunan Univ Chinese Med, Coll Integrated Tradit Chinese & Western Med, Key Lab Hunan Prov Integrated Tradit Chinese & Wes, Changsha, Hunan, Peoples R China.
摘要：
Alzheimer's disease (AD) is a neurodegenerative disorder characterized by complex pathophysiological features. Amyloid plaques resulting from extracellular amyloid deposition and neurofibrillary tangles formed by intracellular hyperphosphorylated tau accumulation serve as primary neuropathological criteria for AD diagnosis. The activation of microglia has been closely associated with these pathological manifestations. Non-coding RNA (ncRNA), a versatile molecule involved in various cellular functions such as genetic information storage and transport, as well as catalysis of biochemical reactions, plays a crucial role in microglial activation. This review aims to investigate the regulatory role of ncRNAs in protein expression by directly targeting genes, proteins, and interactions. Furthermore, it explores the ability of ncRNAs to modulate inflammatory pathways, influence the expression of inflammatory factors, and regulate microglia activation, all of which contribute to neuroinflammation and AD. However, there are still significant controversies surrounding microglial activation and polarization. The categorization into M1 and M2 phenotypes may oversimplify the intricate and multifaceted regulatory processes in microglial response to neuroinflammation. Limited research has been conducted on the role of ncRNAs in regulating microglial activation and inducing distinct polarization states in the context of neuroinflammation. Moreover, the regulatory mechanisms through which ncRNAs govern microglial function continue to be refined. The current understanding of ncRNA regulatory pathways involved in microglial activation remains incomplete and may be influenced by spatial, temporal, and tissue-specific factors. Therefore, further in-depth investigations are warranted. In conclusion, there are ongoing debates and uncertainties regarding the activation and polarization of microglial cells, particularly concerning the categorization into M1 and M2 phenotypes. The study of ncRNA regulation in microglial activation and polarization, as well as its mechanisms, is still in its early stages and requires further investigation. However, this review offers new insights and opportunities for therapeutic approaches in AD. The development of ncRNA-based drugs may hold promise as a new direction in AD treatment.
摘要：
Background: Haglund’s syndrome is a common cause of heel pain but often neglected clinically. Haglund’s syndrome refers to a series of symptoms caused by impingement among posterosuperior prominence of the calcaneus, bursa and Achilles tendon. It is difficult to distinguish Haglund’s syndrome from other causes of heel pain by clinical diagnosis. Imageology is of great value in the diagnosis of Haglund’s syndrome.<&wdkj&>Objective: Our study aims to summarize the Magnet resonance (MR) imaging characteristics of Haglund’s syndrome and provide some reference to clinical work.<&wdkj&>Method: We retrospectively analyzed the MR images of 11 patients (6 males; 5 females; 6 right ankles, 4 left ankles, 1 bimalleolar ankles) who have been clinically and radiologically confirmed Haglund’s syndrome. Observation contents: morphological changes of calcaneus and talus, abnormal signal of calcaneus, abnormal Achilles tendon, and soft tissue abnormalities around Achilles tendon. Combined with literature reviews, summarize the MR imaging features of Haglund’s syndrome.<&wdkj&>Results: In 12 ankles, all ankles showed posterosuperior prominence of the calcaneus and Achilles tendon degeneration; 7 ankles showed bone marrow edema; 6 Achilles tendons were graded as either type II or type III tendinosis; 5 Achilles tendons showed partial tear; 12 ankles showed retrocalcaneal bursitis, 7 ankles showed retro-Achilles bursitis, 6 ankles showed Kager’s fat pad edema.<&wdkj&>Conclusion: This study found that MR images of Haglund's syndrome showed bone edema of the calcaneus, degeneration and partial tear of the Achilles tendon, the retrocalcaneal and retro-Achilles bursas, and Kager’s fat pad edema.
作者：
Jin Ding;Wen Sheng;Baowei Lu;Lumei Liu;Qinghu He
期刊：
Pharmacological Research - Modern Chinese Medicine,2022年5:100188 ISSN：2667-1425
通讯作者：
Lumei Liu<&wdkj&>Qinghu He
作者机构：
[Wen Sheng; Baowei Lu; Lumei Liu] College of Integrated Traditional Chinese and Western Medicine, Hunan University of Chinese medicine, Changsha, China;Department of Andrology Clinic, Shenzhen Bao'an Hospital of Traditional Chinese Medicine, Shenzhen, China;Hu Nan University of Medicine, Huaihua, China;[Jin Ding] College of Integrated Traditional Chinese and Western Medicine, Hunan University of Chinese medicine, Changsha, China<&wdkj&>Department of Andrology Clinic, Shenzhen Bao'an Hospital of Traditional Chinese Medicine, Shenzhen, China;[Qinghu He] College of Integrated Traditional Chinese and Western Medicine, Hunan University of Chinese medicine, Changsha, China<&wdkj&>Hu Nan University of Medicine, Huaihua, China
通讯机构：
[Lumei Liu; Qinghu He] C;College of Integrated Traditional Chinese and Western Medicine, Hunan University of Chinese medicine, Changsha, China<&wdkj&>College of Integrated Traditional Chinese and Western Medicine, Hunan University of Chinese medicine, Changsha, China<&wdkj&>Hu Nan University of Medicine, Huaihua, China
摘要：
Oligoasthenospermia (OAS) remains the leading cause of male infertility. Guilu-Erxian-Glue (GLEXG) is a traditional Chinese formula that exhibits beneficial effects in attenuating reproductive dysfunction. However, the mechanism of GLEXG against OAS remains unclear. In this study, we investigated the therapeutic effects of GLEXG against OAS and analyzed its constituents and underlying mechanism. Using the network pharmacology analysis, 40 targets and 53 bioactive compounds of GLXEG associated with OAS were identified, forming a tight disease-drug-compound-target network. IL-6, MYC, CASP3, EGFR, VEGFA, ERBB2, and ESR1 were screened as hub targets in the protein-protein-interaction network. KEGG and GO enrichment analysis and pathway-target-compound network indicated that the HIF-1 signaling pathways were enriched by numerous targets and interact with multiple pathways, including PI3K/Akt and TNF pathway. Molecular docking analysis revealed a tight binding between potential bioactive compounds and hub targets, and the molecular dynamic simulation of the ESR1-Frutinone A complex with the lowest docking energy exhibited high stability. In addition, an OAS rat model was established to determine the therapeutic effects. The results indicated that GLEXG treatment markedly improved sperm quality in the epididymis and reversed histopathological lesions in the testicular tissue, and regulated the expressions of the predicted hub targets and HIF-1¦Á associated with HIF-1 signaling pathway. Our results illustrated the potential therapeutic effects of GLEXG on OAS, suggesting that it is an alternative therapy against OAS.
期刊：
FOLIA HISTOCHEMICA ET CYTOBIOLOGICA,2022年60(4):323-334 ISSN：0239-8508
通讯作者：
Xiong, Wu
作者机构：
[Xiong, Wu; Lan, Hong-Wei; Zhu, Fu-Rong; Huang, Xin-ling; Wang, Ting-ting; Zhou, Zhong-zhi] Department of Burns and Plastic Surgery, The First Affiliated Hospital of Hunan University of Chinese Medicine, Hunan, China;[Yang, Qian-pei; Hu, Ai -lin; Chen, Zi-Lin; Li, Cheng-Yu; Xia, Yi-fei] College of Integrated Traditional Chinese and Western Medicine, Hunan University of Chinese Medicine, Hunan, China;[Wang, Xiao-Liang] Department of Anesthesia and Operation, The First Affiliated Hospital of Hunan University of Chinese Medicine, Hunan, China
通讯机构：
[Xiong, Wu] D;Department of Burns and Plastic Surgery, the First Affiliated Hospital of Hunan University of Chinese Medicine, Hunan, China.
期刊：
FRONTIERS IN BIOSCIENCE-LANDMARK,2022年27(12) ISSN：2768-6701
通讯作者：
Tian, X.
作者机构：
[Wu, Ruoxia] School of Traditional Chinese Medicine, Hunan University of Chinese Medicine, Hunan, Changsha, 410208, China;[Zhou, Ting] School of Pharmacy, Hunan University of Chinese Medicine, Hunan, Changsha, 410208, China;[Xiong, Jiaqing] Teaching and Research Section of Traditional Chinese Medicine Surgery, First Hospital of Hunan University of Chinese Medicine, Hunan, Changsha, 410021, China;[Tian, Xuefei; Zhang, Zhen] College of Integrated Traditional Chinese and Western Medicine, Hunan University of Chinese Medicine, Hunan, Changsha, 410208, China;[Tian, Sha] Department of Internal Medicine, College of Integrated Traditional Chinese and Western Medicine, Hunan University of Chinese Medicine, Hunan, Changsha, 410208, China
通讯机构：
[Tian, X.] C;College of Integrated Traditional Chinese and Western Medicine, Hunan, China
