作者机构：
[Jing-song Yuan; Gui-hua Gu; Da-jian Yang; Shi-yun Wang; Wei Zeng; Wei Wang] Peking University Shenzhen Hospital;[Jing-song Yuan; Gui-hua Gu; Da-jian Yang; Shi-yun Wang; Wei Zeng; Wei Wang] State Key Laboratory of Chinese Medicine and Molecular Pharmacology (Incubation);[Jing-song Yuan; Gui-hua Gu; Da-jian Yang; Shi-yun Wang; Wei Zeng; Wei Wang] School of Pharmacy, Hunan University of Chinese Medicine
摘要：
Background: The rhizome of Corydalis decumbens （Thunb.） Pers. （Papaveraceae） is one of the well- known TCMs. This medicine has been used for the treatment of hemiplegia, alleviating pain, rheumatic arthritis and infantile paralysis residual. The tertiary and quaternary ammonium alkaloid in it has been investigated to some extent. It is the first report for the simultaneous determination of eleven major alkaloids in Corydalis decumbens （Thunb.） Pers. Objective: A high-performance liquid chromatography with diode-array detection method was developed and validated to determine simultaneously eleven major alkaloids in Corydalis decumbens. The alkaloids detected were: corlumidine, protopine, coptisine, tetrahydrojatrorrhizine, palmatine, berberine, sanguinarine, papaverine hydrochloride, tetrahydropalmatine, bicuculline and corydaline. Materials and Methods: Chromatographic separation was achieved using a C-18 column with a mobile phase composed of A （0.2% acetic acid solution, adjusted with triethylamine to pH 5.0） and B （acetonitrile）, with stepwise gradient elution. Ultraviolet diode-array detection was used; chromatograms were examined at the wavelength of 280 nm. Results: The regression equations showed a good linear relationship between the peak area of each marker and concentration （r = 0.9994-0.9999）. The recovery values ranged between93.66% and 100.54%. The method was fully validated with respect to detection and quantification limits, precision, reproducibility and accuracy. Conclusions: The described HPLC method was successfully used for the differentiation and quantification of the eleven major alkaloids in C. decumbens. and can be considered an effective procedure for the analyses of this important class of natural compounds.
摘要：
Essential hypertension (EH) is a frequent, chronic, age-related disorder, which remains a major modifiable risk factor for cardiovascular disease despite important advances in our understanding of its pathophysiology. Previous studies have noted a consistent maternal effect on blood pressure (BP). Consequently, mutations in mitochondrial DNA (mtDNA) have become an additional target of investigations on the missing BP heritability. Among these mutations, mt-transfer RNA (tRNA) is a hot mutational spot for pathogenic mutations associated with EH. Mutant mtDNA aggravates mitochondrial dysfunction, pivotally contributing to the clinical phenotype. Moreover, the damaged mitochondria, due to their inability to provide the high-energy requirements for cells, generate reactive oxygen species (ROS) and induce mitochondrial-mediated cell death pathways. Therefore, mitochondrial dysfunction plays a critical role in the pathogenesis of EH. This review summarizes the basic knowledge of mitochondrial genetics and EH-associated mtDNA mutations and further discusses the molecular mechanisms behind these mtDNA mutations in clinical manifestations of EH.
作者机构：
( Division of Stem Cell Regulation and Application, State Key Laboratory of Chinese Medicine Powder and Medicine Innovation in Hunan (Incubation) , Hunan University of Chinese Medicine, Changsha, Hunan 410208, China);[Shao-Wei Sun Duan-Fang Liao] 干细胞调控与应用研究室
作者机构：
[罗跃龙] School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China;[万丹; 张水寒; 蔡萍; 肖娟] Key Laboratory of Chinese Materia Medica Research and Development, Hunan Academy of Chinese Medicine, Changsha 410013, China;[严瑞娟] School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China, Key Laboratory of Chinese Materia Medica Research and Development, Hunan Academy of Chinese Medicine, Changsha 410013, China
通讯机构：
[Zhang, S.-H.] K;Key Laboratory of Chinese Materia Medica Research and Development, Hunan Academy of Chinese Medicine, China
