期刊：
Frontiers in Microbiology,2025年16:1560340 ISSN：1664-302X
通讯作者：
Kuang, L
作者机构：
[Li, Ruoyu; Kuang, Lin; Shi, Jialing; Qi, Li; Kuang, L] Hunan Univ Chinese Med, Sch Integrated Chinese & Western Med, Changsha, Peoples R China.;[Wu, Yangfan] Hunan Univ Chinese Med, Xiangxing Coll, Changsha, Peoples R China.
通讯机构：
[Kuang, L ] H;Hunan Univ Chinese Med, Sch Integrated Chinese & Western Med, Changsha, Peoples R China.
关键词：
HSV-2;HaCaT;RNA-seq;TLR9;acyclovir
摘要：
INTRODUCTION: The objective of this study was to investigate the effect of acyclovir (ACV) on the TLR9 signaling pathway after human immortalized epidermal (HaCaT) cell infection with herpes simplex virus type 2 (HSV-2). METHODS: In this study, an in vitro cell model of HSV-2 infection was successfully constructed by infecting HaCaT with HSV-2 virus. In order to explore the antiviral mechanism of acyclovir (ACV), high-throughput transcriptome sequencing (RNA-seq) was used to analyze the genome-wide expression profiling of infected cells before and after ACV treatment, and to systematically compare the change characteristics of differentially expressed genes (DEGs). Based on the sequencing results, the study further focused on Toll-like receptor (TLR) 9 signaling, using quantitative real-time reverse transcriptase chain reaction (qRT-PCR) to quantitatively detect the effect of ACV intervention on the mRNA expression level of key molecules of TLR 9 signaling pathway in HSV-2 infected HaCaT cells. RESULTS: A total of 896 significant changes in gene expression were identified by the transcriptome analysis, including 314 upregulated genes and 582 downregulated genes. GO enrichment analysis showed that the differentially expressed genes were mainly related to CC includes the ubiquitin ligase complex, mitochondrial protein-containing complex, DNA-binding transcription activator activity, exonuclease activity, catabolic process, nuclear-transcribed mRNA catabolic process nuclear-transcribed mRNA catabolic process; KEGG enrichment analysis showed that the differentially expressed genes were mainly related to Toll-like receptor signaling pathway, herpes simplex virus 1 infection, and TNF signaling pathway. The RT-PCR results were confirmed to be basically consistent with the sequencing results. CONCLUSION: ACV altered the transcriptome level of HSV-2 infection in HaCaT cells. The RT-PCR results confirmed that ACV intervened in HSV-2 infection through the TLR9 signaling pathway.
作者机构：
[Li, Jiani; Zhou, Yimiao; Xiao, ZW; Zhou, Liquan; Xiao, Zuowei; Zhou, YM; Ding, Chen] Hunan Univ Chinese Med, Homologous Innovat Lab Med & Food, Changsha 410208, Peoples R China.;[Li, Jiani; Zhou, Yimiao; Xiao, ZW; Zhou, Liquan; Xiao, Zuowei; Zhou, YM; Ding, Chen] Hunan Univ Chinese Med, Hunan Engn & Technol Res Ctr Hlth Prod & Life Sci, Changsha 410208, Peoples R China.;[Li, Jiani; Zhou, Yimiao; Xiao, ZW; Zhou, Liquan; Xiao, Zuowei; Zhou, YM; Ding, Chen] Hunan Univ Chinese Med, Sch Pharm, Changsha 410208, Peoples R China.;[Zhou, Yimiao; Zhou, YM] Hunan Univ Chinese Med, Xiangxing Coll, Xiangyin Campus, Yueyang 414615, Peoples R China.
通讯机构：
[Xiao, ZW ; Zhou, YM ; Zhou, YM] H;Hunan Univ Chinese Med, Homologous Innovat Lab Med & Food, Changsha 410208, Peoples R China.;Hunan Univ Chinese Med, Hunan Engn & Technol Res Ctr Hlth Prod & Life Sci, Changsha 410208, Peoples R China.;Hunan Univ Chinese Med, Sch Pharm, Changsha 410208, Peoples R China.;Hunan Univ Chinese Med, Xiangxing Coll, Xiangyin Campus, Yueyang 414615, Peoples R China.
关键词：
gut microbiota;hypoglycemia;mechanism of action;natural polysaccharides;signaling pathway
摘要：
Natural polysaccharides (NPs), as a class of bioactive macromolecules with multitarget synergistic regulatory potential, exhibit significant advantages in diabetes intervention. This review systematically summarizes the core hypoglycemic mechanisms of NPs, covering structure-activity relationships, integration of the gut microbiota-metabolism-immunity axis, and regulation of key signaling pathways. Studies demonstrate that the molecular weight, branch complexity, and chemical modifications of NPs mediate their hypoglycemic activity by influencing bioavailability and target specificity. NPs improve glucose metabolism through multiple pathways: activating insulin signaling, improving insulin resistance (IR), enhancing glycogen synthesis, inhibiting gluconeogenesis, and regulating gut microbiota homeostasis. Additionally, NPs protect pancreatic β-cell function via the nuclear factor E2-related factor 2 (Nrf2)/Antioxidant Response Element (ARE) antioxidant pathway and Toll-like receptor 4 (TLR4)/nuclear factor-κB (NF-κB) anti-inflammatory pathway. Clinical application of NPs still requires overcoming challenges such as resolving complex structure-activity relationships and dynamically integrating cross-organ signaling. Future research should focus on integrating multi-omics technologies (e.g., metagenomics, metabolomics) and organoid models to decipher the cross-organ synergistic action networks of NPs, and promote their translation from basic research to clinical applications.
摘要：
Background Weight stigma is linked to adverse effects, but whether it directly heightens the risk of disordered eating behaviors or psychological factors mediate this connection among postpartum women is uncertain.
Weight stigma is linked to adverse effects, but whether it directly heightens the risk of disordered eating behaviors or psychological factors mediate this connection among postpartum women is uncertain.
Objectives To investigate the relationship between perceived weight stigma and disordered eating behaviors (restrained eating, emotional eating, and external eating) and identify the mediating role of weight bias internalization and postpartum depression (PPD).
To investigate the relationship between perceived weight stigma and disordered eating behaviors (restrained eating, emotional eating, and external eating) and identify the mediating role of weight bias internalization and postpartum depression (PPD).
Methods This cross-sectional study involved 507 postpartum women. Data were collected anonymously using self-reported questionnaires, including the Perceived Weight Stigma Questionnaire, Weight Bias Internalization Scale, Edinburgh Postpartum Depression Scale, and Dutch Eating Behavior Questionnaire. Data were analyzed using descriptive statistics, Pearson's correlation analysis, hierarchical linear regression, and mediation analysis.
This cross-sectional study involved 507 postpartum women. Data were collected anonymously using self-reported questionnaires, including the Perceived Weight Stigma Questionnaire, Weight Bias Internalization Scale, Edinburgh Postpartum Depression Scale, and Dutch Eating Behavior Questionnaire. Data were analyzed using descriptive statistics, Pearson's correlation analysis, hierarchical linear regression, and mediation analysis.
Results The results showed that the relationship between perceived weight stigma and disordered eating behaviors was fully mediated by weight bias internalization and PPD. Specifically, perceived weight stigma was associated with greater weight bias internalization, which was associated with greater PPD, and greater PPD was associated with greater disordered eating behaviors.
The results showed that the relationship between perceived weight stigma and disordered eating behaviors was fully mediated by weight bias internalization and PPD. Specifically, perceived weight stigma was associated with greater weight bias internalization, which was associated with greater PPD, and greater PPD was associated with greater disordered eating behaviors.
Conclusion This may be the first study to propose a chain mediation model exploring the roles of weight bias internalization and PPD in the relationship between perceived weight stigma and disordered eating behaviors among postpartum women. The findings enhance the understanding of the psychological impacts of perinatal weight stigma and underscore the importance of addressing weight stigma in postpartum care. Comprehensive interventions should be developed to enhance postpartum health and reduce the risk of disordered eating behaviors.
This may be the first study to propose a chain mediation model exploring the roles of weight bias internalization and PPD in the relationship between perceived weight stigma and disordered eating behaviors among postpartum women. The findings enhance the understanding of the psychological impacts of perinatal weight stigma and underscore the importance of addressing weight stigma in postpartum care. Comprehensive interventions should be developed to enhance postpartum health and reduce the risk of disordered eating behaviors.
期刊：
FRONTIERS IN NUTRITION,2025年12:1572189 ISSN：2296-861X
通讯作者：
Xu, Y;Yi, Z
作者机构：
[Zhou, Qian; Tan, Xiaoqin; Zhang, Wanjia; Yang, Weiqing] Hunan Univ Chinese Med, Grad Sch, Changsha, Hunan, Peoples R China.;[Xu, Yin] Hunan Univ Chinese Med, Hosp 1, Dept Gastroenterol, Changsha, Hunan, Peoples R China.;[Yi, Zhan] Hunan Univ Chinese Med, XiangXing Coll, Changsha, Hunan, Peoples R China.
通讯机构：
[Yi, Z ; Xu, Y ] H;Hunan Univ Chinese Med, Hosp 1, Dept Gastroenterol, Changsha, Hunan, Peoples R China.;Hunan Univ Chinese Med, XiangXing Coll, Changsha, Hunan, Peoples R China.
摘要：
BACKGROUND: Metabolic syndrome (MetS) is a global health concern linked to increased mortality. Diets rich in plant-derived compounds, such as polyphenols, have shown potential health benefits for MetS. Among these, flavan-3-ols, a class of commonly occurring polyphenolic compounds, are known for their antioxidant and anti-inflammatory properties. Therefore, we hypothesize that flavan-3-ols intake is negatively associated with mortality risk in MetS population. METHODS: This study analyzed NHANES data (2007-2008, 2009-2010, and 2017-2018). Flavan-3-ol and monomer intake were obtained from the USDA Flavonoid and FNDDS databases. Associations with mortality were assessed using Cox regression, survival differences were compared using Kaplan-Meier curves, and non-linear trends were examined using restricted cubic splines. Subgroup analyses were conducted to explore potential effect modifications. RESULTS: Over a median follow-up period of 114 months, 1,856 participants survived, while 329 deaths were recorded. In Model 3, participants in the highest tertile (T3) of flavan-3-ol intake exhibited a 33% lower risk of all-cause mortality compared to those in the lowest tertile (T1) (HR = 0.67, 95%CI: 0.49-0.92). For monomers, the hazard ratios ranged from 0.55 for higher levels of epigallocatechin to 0.71 for higher levels of gallocatechin. Kaplan-Meier curves indicated significant differences in survival status across dietary flavan-3-ol intake groups. However, no association was found between flavan-3-ol intake and cardiovascular mortality risk. Additionally, restricted cubic spline (RCS) analysis did not reveal any non-linear relationship, and no significant interaction effects were observed in the subgroup analysis. CONCLUSION: Higher dietary intake of flavan-3-ols is negatively associated with mortality risk in MetS population.
摘要：
This paper introduces chief physician of traditional Chinese medicine (TCM) WANG Yigang’s clinical experience in treating peripheral facial palsy in the acute stage with acupuncture-medication-combined therapy. Professor WANG believes that the pathogenesis of facial paralysis in the early stage is mostly the external invasion of wind and pathogenic toxins and the internal disturbance of dampness and toxins, resulting in the obstruction of collaterals and muscle regions of meridians. The treatment should be guided by the “unity of form (body) and spirit (Shen)”, paying attention to the movement of the spirit, dispelling evils, and regulating the spirit. Professor WANG believes that when the spirit initiates, the healthy Qi is strong, and the pathogen subsides. In the treatment, he is good at combining acupuncture and medication for synergistic effect, stresses the use of scalp points, and coins the empirical point Miandong (Extra). At the same time, he does not restrict himself to the traditional needling method and treats facial paralysis with “dynamic retention acupuncture”.
摘要：
Context Aging is a major risk factor for various neurodegenerative diseases, and ferroptosis has been identified as an important mode of cell death during accelerated aging. As the main component of the edible plant YuZhu in China, Polygonatum polysaccharide (POP) is an important natural compound with anti-aging properties.
Aging is a major risk factor for various neurodegenerative diseases, and ferroptosis has been identified as an important mode of cell death during accelerated aging. As the main component of the edible plant YuZhu in China, Polygonatum polysaccharide (POP) is an important natural compound with anti-aging properties.
Objective To evaluate the anti-aging effects of POP and the underlying molecular mechanisms involved and to evaluate the overall anti-aging effects of POP on cognitive impairment due to accelerated aging.
To evaluate the anti-aging effects of POP and the underlying molecular mechanisms involved and to evaluate the overall anti-aging effects of POP on cognitive impairment due to accelerated aging.
Materials and methods A D-galactose (D-gal)-induced accelerated aging rat model was established to evaluate the anti-aging effects of POP and the underlying molecular mechanisms involved. In turn, Morris water maze and open field experiments were used to evaluate the anti-aging effects of POP on cognitive impairment due to accelerated aging.
A D-galactose (D-gal)-induced accelerated aging rat model was established to evaluate the anti-aging effects of POP and the underlying molecular mechanisms involved. In turn, Morris water maze and open field experiments were used to evaluate the anti-aging effects of POP on cognitive impairment due to accelerated aging.
Results The mechanism by which POP affects nuclear factor E2-related factor 2 (Nrf2), an essential transcription factor, was confirmed. POP significantly improved d-gal-induced cognitive dysfunction in treated model rats, which exhibited reduced pathological changes in the hippocampus, reduced latency of the water maze platform, and increased exploration time in the central area in the open field experiment compared to those of untreated model rats. Furthermore, POP intervention downregulated ferroptosis-related proteins and upregulated Nrf2 expression, and selective inhibition of Nrf2 eliminated the ability of POP to reduce ferroptosis.
The mechanism by which POP affects nuclear factor E2-related factor 2 (Nrf2), an essential transcription factor, was confirmed. POP significantly improved d-gal-induced cognitive dysfunction in treated model rats, which exhibited reduced pathological changes in the hippocampus, reduced latency of the water maze platform, and increased exploration time in the central area in the open field experiment compared to those of untreated model rats. Furthermore, POP intervention downregulated ferroptosis-related proteins and upregulated Nrf2 expression, and selective inhibition of Nrf2 eliminated the ability of POP to reduce ferroptosis.
Conclusions POP is a natural ingredient with therapeutic potential due to its ability to alleviate aging by activating Nrf2, inhibiting ferroptosis, and alleviating cognitive dysfunction.
POP is a natural ingredient with therapeutic potential due to its ability to alleviate aging by activating Nrf2, inhibiting ferroptosis, and alleviating cognitive dysfunction.
关键词：
骨质疏松症, 抵抗素, 孟德尔随机化分析, 单细胞RNA测序, 骨密度, osteoporosis, RETN, Mendelian randomization, single-cell RNA sequencing, bone mineral density
摘要：
骨质疏松症是一种最常见的全身性、代谢性的骨骼疾病。血清抵抗素（RETN）的浓度与骨密度（BMD）相关联，但是RETN与BMD和骨折是否存在因果关系仍不清楚。本研究采用孟德尔随机化（MR）分析RETN基因表达与BMD和骨折的因果关系，然后在股骨颈骨折（FNF）和骨关节炎（OA）样本的单细胞RNA测序数据中分析RETN基因的差异表达。MR分析发现，RETN高表达会导致BMD降低和骨折风险增加。单细胞测序分析表明，RETN在FNF组中表达上调，主要由中性粒细胞表达，其相关的炎症趋化因子CXCL8在FNF组中显著高表达。本研究提示，RETN在骨质疏松发病过程中发挥了重要作用。这一结论为骨质疏松的诊断和治疗提供了基础。 您的浏览器不支持 audio 元素。 AI语音播报 Osteoporosis is the most common systemic and metabolic bone disease. The concentration of serum resistin (RETN) is associated with bone mineral density, but it is still unclear whether there is a causal relationship between RETN and bone density and fractures. This study used Mendelian randomization (MR) to analyze the causal relationship between RETN gene expression and bone mineral density and fractures, and then analyzed the differential expression of RETN gene in single-cell sequencing data of femeral neck fracture (FNF) and osteoarthritis (OA) samples. MR analysis showed that high expression of RETN was found to lead to decreased bone mineral density and increased risk of fractures. RETN expression was upregulated in the FNF group, and RETN was mainly expressed by neutrophils. The inflammatory chemokine CXCL8 related to RETN was significantly upregulated in the FNF group. This study suggested the role of RETN in the pathogenesis of osteoporosis, providing a basis for the diagnosis and treatment of osteoporosis.
摘要：
目的: 基于网络药理学预测沙棘治疗酒精性肝损伤（alcohol liver injury）的作用机制，并通过建立斑马鱼酒精性肝损伤动物模型来验证沙棘治疗酒精性肝损伤的功效。方法: 通过中药系统药理学数据库与分析平台（Traditional Chinese Medicine Systems Pharmacology ，TCMSP）、Unipro数据库收集有效成份及其作用靶标，Venny2.1寻找交集靶点。通过GeneCards及OMIM数据库收集和筛选疾病靶点，STRING v12.0数据库进行PPI网络分析，PDB及PubChem进行蛋白质结构及小分子结构确认。通过Cytoscape（Version 3.9.1）软件网络图构建沙棘治疗酒精性肝损伤关联网络。利用Metascape数据库对共有靶点进行基因本体（gene ontology，GO）、京都基因与基因组百科全书（Kyoto encyclopedia of gene，KEGG）数据库通路富集分析。通过斑马鱼实验进行功能验证：选取受精后3天（3 dpf）野生型AB品系斑马鱼，正常组喂养于正常饲养用水，其余各组饲养于2%的无水乙醇溶液中建立酒精性肝损伤模型。沙棘组给予不同浓度的沙棘溶液，28℃处理2天后，确定沙棘对酒精性肝损伤模型斑马鱼的最大耐受浓度（MTC）剂量，并根据MTC结果进行下一步给药，测定样品肝保护功效评价表型实验结果。结果: 筛选后得到沙棘活性成分33个，主要包括槲皮素、花葵素、儿茶酸等；治疗酒精性肝损伤的潜在靶点1434个，主要包括ADH1C、CTNNB1、TGFB1等。调控这些核心靶点的信号通路主要富集在Lipid and atherosclerosis、Fluid shear stress and atherosclerosis、PI3K-Akt signaling pathway等多条信号通路中。动物实验结果显示：与模型组相比，沙棘具有降低酒精性肝损伤模型斑马鱼肝脏不透明值（P＜0.01），改善肝脏和卵黄囊肿大现象（P＜0.01），下调AST和ALT的活力值（P＜0.001），改善肝细胞核肿大，减少肝组织脂肪空泡样变性的功效。结论: 沙棘可改善酒精性肝损伤，其机制可能与改善脂肪酸氧化、细胞代谢和抑制细胞凋亡有关。
作者机构：
[易展; 贲定严] Xiangxing College, Hunan University of Chinese Medicine, Changsha 410208, China;[王佳怡; 徐寅] Department of Internal Medicine, the First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha 410000
