作者： 周晓玲;唐农;吴腾;张悦;陈峭;...

期刊： 中国针灸,2020年40(1):59-66 ISSN：0255-2930

作者机构： [周晓玲] Graduate School of Hunan University of CM, Changsha 410000, China;[陈峭; Qin, Feng-Chuan; 吴腾; 张悦; 张丹璇; 周娅妮; 周晓玲; 杨薇] Liuzhou Hospital of TCM, Liuzhou 545000, Guangxi Province;[唐农; 韦宛华] Guangxi University of CM, Nanning 530000

关键词： 非酒精性脂肪性肝病;非酒精性脂肪性肝炎;背俞穴;穴位埋线;针刺;IKK/IKB/NF-κB信号通路

摘要： 目的:基于IKK/IKB/NF-κB信号通路及下游炎性因子,探讨背俞穴穴位埋线干预非酒精性脂肪性肝炎(nonalcoholic steatohepatitis,NASH)大鼠的机制。方法:80只SPF级SD大鼠,随机取10只作为正常组,剩余70只高脂饲料喂养制备NASH大鼠模型,12周末随机取10只验证造模是否成功。余60只随机分为模型组、背俞穴埋线组、腹部穴埋线组、背俞穴针刺组、假埋线组和西药组,每组10只。背俞穴埋线组、腹部穴埋线组、背俞穴针刺组分别予相应取穴治疗,背俞穴取“肝俞”“脾俞”“胃俞”“肾俞”,腹部穴取“大横”“腹结”“滑肉门”“天枢”;假埋线组在背俞穴处刺激,但不进入皮下组织,不留线;西药组给予维生素E软胶囊灌胃,疗程均4周。正常组不予任何干预,正常饲料喂养至第16周末;模型组继续予高脂饲料喂养至第16周末。干预结束后,计算各组大鼠肝指数;HE染色观察各组大鼠肝脏组织形态;血清生化检测大鼠肝功能[丙氨酸氨基转移酶(ALT)、 γ-谷氨酰转肽酶(γ-GGT)、碱性磷酸酶(ALP)]及血脂[血清总胆固醇(TC)、三酰甘油(TG)、低密度脂蛋白(LDL)]水平;ELISA法检测血清肿瘤坏死因子α(TNF-α)、白介素6(IL-6)和白介素1β(IL-1β)含量;Western blot法检测肝组织IKK-α、NF-κBp65、IL-6、IL-1β、TNF-α蛋白表达;红外热像仪检测大鼠任督脉温度。结果:与正常组比较,模型组大鼠脂肪变性、炎性细胞浸润明显,大鼠体质量、肝脏湿重、肝指数均增加(P <0.01);与模型组比较,背俞穴埋线组、腹部穴埋线组、背俞穴针刺组、西药组肝组织形态均有不同程度改善,肝指数下降(P <0.05),其中以背俞穴埋线组最明显(P <0.05)。与正常组比较,模型组大鼠ALT、γ-GGT、ALP、TG、TC、 LDL、TNF-α、IL-6、IL-1β升高(P<0.01);各干预组与模型组比较,ALT、γ-GGT、ALP、TG、TC、LDL、TNF-α、 IL-6、IL-1β均不同程度下降(P <0.01,P<0.05),其中背俞穴埋线组改善显著优于其他干预组(P <0.01)。模型组IKK-α、NF-κBp65、TNF-α、IL-6、IL-1β蛋白表达较正常组升高(P <0.01);各干预组与模型组比较,均有不同程度下降(P <0.05),背俞穴埋线组改善效果优于其他干预组(P <0.01)。模型组任督二脉温度较正常组降低(P <0.01);背俞穴埋线组、腹部穴埋线组、背俞穴针刺组大鼠任督脉温度与模型组比较,均有提高(P <0.01);背俞穴埋线组任脉温度与腹部穴埋线组比较差异无统计学意义(P >0.05);背俞穴埋线组督脉温度改善优于腹部穴埋线组(P <0.05)。结论:背俞穴埋线可能通过抑制IKK/IKB/NF-κB信号通路的活化,中断炎性因子引起的“瀑布样”效应,减少炎性因子对肝脏的“二次打击”,从而阻断NASH进展,发挥防治NASH的作用。

语种： 中文

作者： Zhou, Xiaoling;Yang, Jianqing;Liu, Ying;Li, Zepeng;Yu, Jingfang;...

期刊： Brazilian Journal of Medical and Biological Research,2019年52(3):e7879 ISSN：0100-879X

通讯作者： Tang, Nong

作者机构： [Zhou, Xiaoling] Hunan Univ Tradit Chinese Med, Grad Sch, Changsha, Hunan, Peoples R China.;[Chen, Qiao; Liu, Ying; Yu, Jingfang; Li, Zepeng; Zhou, Xiaoling; Li, Can] Liuzhou Tradit Chinese Med Hosp, Dept Gastroenterol, Liuzhou, Guangxi, Peoples R China.;[Yang, Jianqing] Liuzhou Tradit Chinese Med Hosp, Dept Surg, Liuzhou, Guangxi, Peoples R China.;[Wei, Wanhua; Tang, Nong] Guangxi Univ Tradit Chinese Med, Nanning, Guangxi, Peoples R China.

通讯机构： [Tang, Nong] G;Guangxi Univ Tradit Chinese Med, Nanning, Guangxi, Peoples R China.

关键词： Bone marrow mesenchymal stem cells;Transplantation;Liver cirrhosis;Granulocyte colony-stimulating factor;Jisheng Shenqi decoction

摘要： Bone marrow mesenchymal stem cells (BMSCs) transplantation has attracted attention for the treatment of liver cirrhosis and end-stage liver diseases. Therefore, in this study, we evaluated the effect of different methods of BMSCs transplantation in the treatment of liver cirrhosis in rats. Seventy-two male Sprague-Dawley rats were divided into 7 groups: 10 were used to extract BMSCs, 10 were used as normal group, and the remaining 52 rats were randomly divided into five groups for testing: control group, BMSCs group, BMSCs+granulocyte colony-stimulating factor (G-CSF) group, and BMSCs+Jisheng Shenqi decoction (JSSQ) group. After the end of the intervention course, liver tissue sections of rats were subjected to hematoxylin and eosin (H&E) and Masson staining, and pathological grades were scored. Liver function [aminotransferase (ALT), aspartate aminotransferase (AST), albumin (ALB)] and hepatic fibrosis markers [hyaluronidase (HA), laminin (LN), type III procollagen (PCIII), type IV collagen (CIV)] were measured. BMSCs+JSSQ group had the best effect of reducing ALT and increasing ALB after intervention therapy (Po0.05). The reducing pathological scores and LN, PCIII, CIV of BMSCs+G-CSF group and BMSCs+JSSQ group after intervention therapy were significant, but there was no significant difference between the two groups (P40.05). The effect of JSSQ on improving stem cell transplantation in rats with liver cirrhosis was confirmed. JSSQ combined with BMSCs could significantly improve liver function and liver pathology scores of rats with liver cirrhosis. © 2019, Associacao Brasileira de Divulgacao Cientifica. All rights reserved.

语种： 英文

作者： 熊家青;刘丽芳;李逵;张伟霞;聂佳欣

期刊： 中南大学学报(医学版),2018年43(3):246-252 ISSN：1672-7347

通讯作者： Liu, L.

作者机构： [熊家青] Graduate School of Hunan University of Traditional Chinese Medicine, Changsha 410208, China;[刘丽芳; 李逵] Department of Breast Surgery, First Affiliated Hospital of Hunan University of Traditional Chinese Medicine, Changsha 410007, China;[张伟霞; 聂佳欣] Graduate School of Hunan University of Traditional Chinese Medicine, Changsha 410208

通讯机构： Department of Breast Surgery, First Affiliated Hospital of Hunan University of Traditional, Chinese Medicine, Changsha, China

关键词： Notch信号通路;深II度烧伤

摘要： 目的:探讨Notch信号通路与兔深II度烧伤模型创面血管相关因子表达的关系。方法:120只新西兰大白兔,随机分为阻滞剂组、模型组、对照组。其中阻滞剂组于造模前1 d及造成深II度烫伤后连续14 d按2 mg/kg注射γ-分泌酶阻滞剂(gamma-secretase inhibitor,GSI),每天1次;模型组同期注射生理盐水,每天1次;对照组不予造模,余同模型组。每组分别于第3,7,14,21,28天随机抽取8只处死,采用免疫组织化学法检测血管内皮生长因子(vascular endothelial growth factor,VEGF)、血管内皮细胞生长因子受体2(vascular endothelial growth factor receptor 2,VEGFR-2)、基质金属蛋白酶2(matrix metalloprotein 2,MMP-2)、基质金属蛋白酶9(matrix metalloprotein 9,MMP-9)的表达。结果:模型组与阻滞剂组造模后21 d内VEGF和VEGFR-2的表达逐步上升,第21天后有所下降,而MMP-2和MMP-9的表达逐步下降,第21天后有所上升,与对照组比较,差异均有统计学意义(P<0.05);各时间点模型组VEGF和VEGFR-2的表达均高于阻滞剂组,MMP-2和MMP-9的表达均低于阻滞剂组,差异具有统计学意义(P<0.05),阻滞剂组在造模后21 d内, VEGFR-2的表达与VEGF呈正相关,而MMP-2和MMP-9则与VEGF呈负相关。结论:在兔深II度烧伤模型创面中,Notch信号通路被阻滞能够减弱创面血管生成因子VEGF和VEGFR-2的表达,而上调抑制因子MMP-2和MMP-9的表达。

语种： 中文

作者： 李翠英;李金香;潘诗敏;张曦;李莹

期刊： 中国针灸,2017年37(8):799-804 ISSN：0255-2930

作者机构： [李莹; 潘诗敏; 李翠英; 张曦] the First Affiliated Hospital of Hunan University of CM, Changsha 410007, China;[李莹; 潘诗敏; 李翠英; 张曦] Graduate School of Hunan University of CM, Changsha 410208;[李金香] the First Affiliated Hospital of Hunan University of CM, Changsha 410007, China. 1306550930@qq.com

关键词： 消化性溃疡;五经配伍;五行生克制化理论;针刺疗法

摘要： 目的:比较基于湖湘五经配伍理论针刺与常规西药治疗消化性溃疡的疗效差异。方法:将60例消化性溃疡患者根据中医辨证分为肝胃不和型及脾胃虚弱型两个区组,然后将两个区组内的患者随机分为观察组与对照组,最终有效病例57例,观察组28例(其中肝胃不和型17例,脾胃虚弱型11例),对照组29例(其中肝胃不和型18例,脾胃虚弱型11例)。观察组按湖湘五经配伍理论指导制定方案,予以毫针针刺治疗,肝胃不和型选取中脘、太冲、行间、期门、足三里、公孙、少府、经渠、内关穴;脾胃虚弱型选取中脘、大都、太白、阴陵泉、足三里、少府、太冲、阴谷、太溪、太渊穴,辨证施予补泻操作,每次留针30 min,每天1次,每周治疗5 d,休息2 d。对照组采用西药口服治疗,其中幽门螺杆菌(Hp)阳性者予以常规三联药物奥美拉唑+阿莫西林+克拉霉素口服,Hp阴性者直接口服奥美拉唑。两组均治疗4周,观察两组患者治疗前、治疗后及治疗后1个月临床证候积分,比较两组患者胃镜下溃疡愈合情况、抗Hp感染及转阴率,并评定两组总体疗效。结果:在治疗后及治疗后1个月,两组患者临床证候积分与本组治疗前比较均有下降(均P <0.05),组间比较差异无统计学意义(均P >0.05);组内不同证型患者比较证候积分差异均无统计学意义(均P >0.05)。两组胃镜下溃疡愈合总有效率、抗Hp感染总有效率及临床总有效率比较差异均无统计学意义(均P>0.05);观察组Hp转阴率为22.2%(4/18),对照组为52.6%(10/19),观察组转阴率低于对照组(P<0.05)。结论:基于湖湘五经配伍理论体系针刺治疗消化性溃疡,能有效改善患者的临床证候,促进溃疡的愈合,有效抗Hp感染,其临床总体疗效与西医常规药物治疗基本相当,但在Hp的转阴率上不及西医常规药物治疗。

语种： 中文

作者： 唐梅森;龚后武;黄政德（黄政德）;晏峻峰;李杰

期刊： 中国中西医结合杂志,2017年37(4):492-494 ISSN：1003-5370

作者机构： 湖南中医药大学研究生院, 长沙, 410208;湖南中医药大学中医学院, 长沙, 410208;[黄政德; 唐梅森; 龚后武; 晏峻峰] 湖南中医药大学研究生院, 长沙, 410208;[李杰] 湖南中医药大学中医学院, 长沙, 410208

关键词： 冠心病;血瘀证;症状;中药;最大频繁关联模式

摘要： 收集中医治疗冠心病血瘀证医案121例,建立冠心病血瘀证诊疗数据库,运用数据挖掘探讨中医治疗冠心病血瘀证的症状与用药的内在规律,探索冠心病血瘀证症状与药物组成的最大频繁关联模式。在所整理的121例医案中,胸痛、胸闷及头痛是冠心病血瘀证患者的常见症状,活血药、化瘀药及行气药之间的配伍是最常用的药物组合,二者之间的关联构成了冠心病血瘀证常见的最大频繁关联模式。以关联模式挖掘所得的冠心病血瘀证症状与药物关联模式反应了治疗冠心病血瘀证标本同治的治疗思想。

语种： 中文

作者： Wang, Shunmin;Tang, Lujun;Zhou, Qian;Lu, Duomei;Duan, Wulei;...

期刊： DNA and Cell Biology,2017年36(5):377-385 ISSN：1044-5498

通讯作者： Tan, Yuansheng

作者机构： [Tang, Lujun; Zhou, Qian; Chen, Cheng; Lu, Duomei; Duan, Wulei; Wang, Shunmin] Hunan Univ Chinese Med, Grad Inst, Changsha, Hunan, Peoples R China.;[Huang, Lu; Wang, Shunmin] Hunan Univ Chinese Med, Hosp 1, Dept Cardiovasc, Changsha, Hunan, Peoples R China.;[Tan, Yuansheng] Hunan Acad Chinese Med, Coll Tradit Chinese Med 1, Dept Cardiovasc, Changsha, Hunan, Peoples R China.;[Tan, Yuansheng] Hunan Acad Chinese Med, Coll Integrated Tradit Chinese & Western Med, Dept Cardiovasc, Changsha, Hunan, Peoples R China.;[Tan, Yuansheng] Hunan Univ Chinese Med, Hanpu Sci & Technol Pk, Changsha 410208, Hunan, Peoples R China.

通讯机构： [Tan, Yuansheng] H;Hunan Univ Chinese Med, Hanpu Sci & Technol Pk, Changsha 410208, Hunan, Peoples R China.

关键词： miR-185;human aortic vascular smooth muscle cells;angiotensin II;P2Y6;proliferation;hypertension

摘要： The abnormal proliferation and apoptosis of human aortic vascular smooth muscle cells (HAVSMCs) play an important role in the pathogenesis of hypertension. Recent study revealed that angiotensin II (Ang II) could elicit HAVSMC dysfunction, to induce or aggravate hypertension. Purinergic receptor P2Y6, an inflammation-inducible G protein-coupled receptor, promoted Ang II-induced hypertension. In the present study, we revealed that Ang II induced HAVSMC proliferation and upregulated P2Y6 protein levels. After knockdown of P2Y6, the promotive effect of Ang II on HAVSMC proliferation was restored. microRNAs (miRNAs) involve in most biological processes. In this study, we scanned out seven candidate miRNAs, which were predicted to contain binding site of P2Y6's 3′-UTR by online tools. Among them, miR-185 was significantly downregulated by Ang II treatment. miR-185 reduced P2Y6 protein levels by direct binding to the 3′UTR of P2Y6. miR-185 overexpression suppressed HAVSMC proliferation; P2Y6 overexpression or Ang II treatment promoted HAVSMC proliferation, and restored the suppressive effect of miR-185 on HAVSMC proliferation. Besides, miR-185/P2Y6 axis also affected pERK1/2 protein levels. Taken together, the present study indicated that miR-185/P2Y6 axis might inhibit Ang II-induced HAVSMC proliferation through miR-185 negatively regulating P2Y6 expression and the downstream ERK pathway; rescuing miR-185 expression to inhibit P2Y6 may represent a therapeutic strategy against HAVSMC dysfunction and hypertension. © Copyright 2017, Mary Ann Liebert, Inc. 2017.

语种： 英文

作者： 张程程;林亚平;彭艳;岳增辉;陈海交;...

期刊： 针刺研究,2017年42(6):482-488 ISSN：1000-0607

作者机构： [杨建文; 刘丽; 刘薇薇; 陈海交; 张程程] Graduate School, Hunan University of Chinese Medicine, Changsha 410007, China;[林亚平; 彭艳; 岳增辉] Key Acu-moxibustion Laboratory of Biological Information Analysis of Institute of Acupuncture, Moxibustion and Massage, Hunan University of Chinese Medicine, Changsha 410007, China

关键词： 电针;糖尿病胃轻瘫;胃肠动力;Cajal间质细胞

摘要： 目的:观察电针对糖尿病胃轻瘫(DGP)大鼠胃肠运动及其胃窦起搏细胞Cajal间质细胞(ICC)超微结构、c-kit受体蛋白表达及干细胞因子(SCF)基因表达的影响,探讨电针治疗DGP的机制。方法:SD大鼠随机分为空白对照组、模型组、电针穴位组、电针非穴组、胃复安对照组,每组10只。腹腔注射2%链脲佐菌素结合高糖高脂饲料不规则喂养制备DGP模型。电针穴位组电针"足三里""梁门""三阴交",电针非穴组电针"足三里""梁门""三阴交"穴位对照点,胃复安对照组予1.7%胃复安药液(1 mL/100 g)灌胃,均每日1次,连续15 d。监测血糖,酚红灌胃法测量大鼠胃排空率及小肠推进率,透射电镜法检测胃窦ICC超微结构,Western blot法、RT-PCR法分别检测大鼠胃窦c-kit蛋白及SCF mRNA表达水平。结果:与空白对照组比较,模型组大鼠血糖显著升高(P<0.01),胃排空率和小肠推进率显著降低(P<0.01),ICC呈细胞凋亡样改变,SCF mRNA表达量显著下降(P<0.01);与模型组比较,电针穴位组大鼠血糖明显降低(P<0.05),胃排空率及小肠推进率显著升高(P<0.05,P<0.01),ICC数目增加,受损超微结构得到修复,SCF mRNA表达量显著升高(P<0.01);与电针非穴组比较,电针穴位组ICC受损超微结构有所恢复,SCF mRNA表达量明显升高(P<0.05)。各造模组间比较,c-kit蛋白表达水平差异无统计学意义(P>0.05)。结论:电针"足三里"等穴可调控DGP大鼠血糖,促进胃肠排空,其作用机制可能与上调SCF mRNA,修复受损ICC超微结构,恢复其起搏功能,进而改善胃肠运动障碍有关。

语种： 中文

作者： Yang, Zhen;Zhu, Mei-lin;Li, De-hai*;Zeng, Rong*;Han, Bing-nan*

期刊： Bioorganic & Medicinal Chemistry,2017年25(24):6614-6622 ISSN：0968-0896

通讯作者： Zeng, Rong;Han, Bing-nan;Li, De-hai

作者机构： [Yang, Zhen; Zeng, Rong] Hunan Univ Chinese Med, Grad Sch, Dept Pharm, Changsha 410208, Hunan, Peoples R China.;[Han, Bing-nan] Zhejiang Sci Tech Univ, Coll Life Sci, Dept Dev Technol Marine Resources, Hangzhou 310018, Zhejiang, Peoples R China.;[Zhu, Mei-lin; Li, De-hai] Ocean Univ China, Key Lab Marine Drugs, Chinese Minist Educ, Sch Med & Pharm, Qingdao 266003, Peoples R China.;[Li, De-hai] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Drugs & Bioprod, Qingdao 266237, Peoples R China.

通讯机构： [Zeng, Rong] H;[Han, Bing-nan] Z;[Li, De-hai] O;Hunan Univ Chinese Med, Grad Sch, Dept Pharm, Changsha 410208, Hunan, Peoples R China.;Zhejiang Sci Tech Univ, Coll Life Sci, Dept Dev Technol Marine Resources, Hangzhou 310018, Zhejiang, Peoples R China.

关键词： *HO-1;*N-Me-trichodermamide B;*Nrf2;*Oxidative stress;*p38

摘要： A new trichodermamide-like alkaloid, N-Me-trichodermamide B (compound 1), was isolated from a marine fungus Penicillium janthinellum HDN13-309. The structure and absolute configuration of compound 1 were determined by extensive NMR analysis and the modified Mosher's method. This new alkaloid exhibited cellular protection from the H2O2-induced oxidative damage, and the mechanism study revealed that this antioxidant activity was regulated through Nrf2-mediated signaling pathway in HaCaT human keratinocytes. In addition, the inhibitor of p38 abrogated compound 1-induced phosphorylation of p38, up-expression of HO-1, and the nuclear localization of Nrf2. As a result, it suggested that this new alkaloid-induced antioxidant signaling pathway might be initiated through activation of p38. (C) 2017 Elsevier Ltd. All rights reserved.

语种： 英文

作者： 杨建文;彭艳;陈海交;张程程;刘薇薇;...

期刊： 针刺研究,2017年42(4):315-320 ISSN：1000-0607

作者机构： [杨建文; 刘丽; 刘薇薇; 陈海交; 张程程] Graduate School, Hunan University of Traditional Chinese Medicine, Changsha 410208, China;[彭艳; 刘密] Key Lab for Acu-moxibustion Biological Information Analysis of College of Acu-moxibustion and Massage, Hunan University of Traditional Chinese Medicine, Changsha 410208, China;[林亚平] Key Lab for Acu-moxibustion Biological Information Analysis of College of Acu-moxibustion and Massage, Hunan University of Traditional Chinese Medicine, Changsha 410208, China. lyp5381161@126.com

关键词： 电针;糖尿病胃轻瘫;胃动力;胰岛素样生长因子1;胰岛素样生长因子1受体;胃窦

摘要： 目的:观察电针"足三里""梁门""三阴交"对糖尿病胃轻瘫(DGP)大鼠胃肠动力、胃窦胰岛素样生长因子1(IGF-1)及其受体(IGF-1 R)表达的影响,探讨电针治疗DGP的可能机制。方法:SD大鼠随机分为空白对照组、模型对照组、电针腧穴组、电针非穴组、胃复安对照组,每组10只。采用腹腔注射2%链脲佐菌素(55 mg/kg)结合高脂高糖不规律饮食制备DGP模型。电针腧穴组取单侧"足三里""梁门""三阴交"电针治疗,电针非穴组取同侧三穴对照点电针治疗,胃复安对照组予1.7%胃复安药液(1 mL/100 g)灌胃,均每天1次,连续干预15 d。观察各组大鼠症状积分、血糖、胃排空率、小肠推进率,ELISA法检测胃窦组织IGF-1及IGF-1 R的含量。结果:与空白对照组比较,模型对照组胃排空率及小肠推进率明显降低,血糖、症状积分、胃窦组织IGF-1及IGF-1 R含量明显升高(P<0.01)。与模型对照组比较,电针腧穴组、胃复安对照组胃排空率及小肠推进率明显升高(P<0.05,P<0.01),电针腧穴组血糖、症状积分、胃窦组织IGF-1及IGF-1 R含量明显降低(P<0.05,P<0.01)。与电针腧穴组比较,电针非穴组及胃复安对照组症状积分、胃窦组织IGF-1及IGF-1 R含量均明显升高(P<0.05,P<0.01)。结论:电针腧穴能降低DGP大鼠血糖水平,促进胃肠动力,其作用机制可能与降低胃窦组织IGF-1及IGF-1 R含量有关。

语种： 中文

作者： 郭军华;李迎秋;郭炫佐;刘朝圣;何清湖

期刊： 中华男科学杂志,2017年23(8):722-727 ISSN：1009-3591

作者机构： [郭军华; 刘朝圣; 李迎秋; 郭炫佐; 何清湖] Graduate School, Hunan University of Chinese Medicine, Changsha, Hunan 410007, China;[郭军华] Department of Andrology, YanCheng Hospital of Traditional Chinese Medicine, YanCheng, Jiangsu 224002, China

关键词： 解脲脲原体;细胞色素氧化酶;知柏地黄汤;mRNA表达

摘要： 目的:探讨解脲脲原体(UU)感染对大鼠生精细胞线粒体细胞色素氧化酶(COX)的影响以及知柏地黄汤对其的干预作用。方法: 4~5月龄SD雄性大鼠40只,随机抽取30只,经膀胱接种UU悬液,建立UU睾丸感染动物模型。剩余10只同步注射生理盐水作为正常组,于接种后的第7天取睾丸穿刺液运用培养法检测UU,判断造模成功否。造模成功后,UU感染模型大鼠再随机分成模型组、知柏地黄汤组、阿奇霉素组。知柏地黄汤组给药量为1 g/(kg·d),阿奇霉素组给药量为0.105 mg /(kg·d),正常组、模型组给予相同剂量的生理盐水。以上各组均灌胃给药,每日1次,连续给药21 d(1 ml/d)。采用颈椎脱臼法处死大鼠,取附睾组织,采用机械分离法分离精子细胞,采用彩色精子动态检测系统检测精子运动参数;取睾丸组织,采用免疫组化法(DAB法)检测COX活性,RT-PCR法检测COXⅠ、Ⅱ mRNA表达。结果:模型组大鼠a级精子,b级精子,直线速度,平均速度,COX Ⅰ、Ⅱ mRNA表达均明显低于正常组(P<0.05, P<0.01)。经治疗后,知柏地黄汤组和阿奇霉素组a级精子、b级精子、直线速度、曲线速度、平均速度水平及COXⅠmRNA表达均提高,与模型组比较,差异有统计学意义(P<0.05,P<0.01),分别为a级精子[(1.11±0.30)、(0.60±0.19)、(0.07±0.03)%]、b级精子[(2.40±0.59)、(1.32±0.27)、(0.35±0.13)%]、直线速度[(12.11±1.62)、(11.47±1.21)、(6.78±1.05) μm/s]、曲线速度[(54.30±2.35)、(45.75±1.64)、(38.10±7.65) μm/s]、平均速度[(18.40±1.27)、(16.69±1.02)、(10.02±1.08)μm/s]、COXⅠ mRNA[(1.86±0.30)、(1.74±0.17)、(0.93±0.10)%],而两组均不能提高COX活性和COXⅡ mRNA表达,知柏地黄汤组在提高a级精子、b级精子、曲线速度、平均速度方面效果优于阿奇霉素组,差异有统计学意义(P<0.05,P<0.01)。结论: UU感染能够降低大鼠a级精子,b级精子,直线速度,平均速度水平,COXⅠ、Ⅱ mRNA表达。知柏地黄汤和阿奇霉素对其都有治疗作用,且知柏地黄汤的效果优于阿奇霉素。精子活力的提高可能与COX活性无关。COX活性可能与COXⅡ mRNA有关,与COXⅠ mRNA表达无关。

语种： 中文

作者： 林群芳;黄培;商学军;田雪飞;张国民;...

期刊： 中华男科学杂志,2017年23(2):157-163 ISSN：1009-3591

作者机构： [林群芳; 刘慧英; 黄培] Graduate School, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;[商学军] Department of Andrology, Jinling Hospital, Nanjing University School of Medicine, Nanjing, Jiangsu,210002, China;[田雪飞; 张国民] School of Integrated Chinese and Western Medicine, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;[周青; 高瑞松; 周兴] Department of Andrology, The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China

关键词： 麝香;乳香;前列腺干细胞;泌尿生殖窦间质细胞;小鼠

摘要： 目的:研究麝香、乳香配伍处理对前列腺干细胞增殖分化的影响。方法:选用7~10天龄的C57BL/6雄性幼鼠和16~18天龄的孕鼠,分别分离培养出前列腺上皮细胞和泌尿生殖窦间质(UGSM)细胞,并将二者混悬液移植到SCID~CB.17雄性小鼠的肾包膜下,30 d后收获移植物。将SCID~(CB.17)小鼠随机分为4组:麝香组、乳香组、麝香+乳香组、空白对照组,每组8只,按组别分别予麝香、乳香、麝香+乳香、生理盐水连续灌胃14 d后,取肾脏组织。通过HE染色形态学观察麝香配伍乳香对前列腺干细胞移植后腺管分化形成、各亚群干细胞分化状态影响,免疫组化和Western印迹检测前列腺干细胞抗原标志物P63、CD133、CD117、sca-1的表达分布情况。结果:建立了前列腺干细胞Lin- Sca-1~+CD49f~+(LSC)细胞及小鼠胚胎UGSM细胞的分离、混合培养体系;免疫组化结果示类前列腺腺泡上皮样结构P63、CD133、Sca-1、CD117染色呈阳性,证实移植物中可见前列腺腺泡上皮结构,且乳香组、麝香组的表达较空白对照组均有不同程度的增高,其中麝香+乳香组CD133、Sca-1、CD117免疫组化的积分光密度(IOD)值均显著高于单用乳香组和空白对照组(均<0.05),同时Sca-1的表达水平也显著高于单用麝香组(P<0.05),而P63的表达水平4组间无显著差异。Western印迹结果也显示,麝香+乳香组CD133、Sca-1、CD117的相对表达量均显著高于单用乳香组和空白对照组(P<0.05或<0.01),Sca-1的表达水平也显著高于单用麝香组(P<0.01)。结论:麝香、乳香配伍使用能促进前列腺干细胞的增殖再生。

语种： 中文

作者： 潘诗敏;李金香;张曦;李莹;严洁;...

期刊： 中国针灸,2017年37(12):1255-1260 ISSN：0255-2930

作者机构： [李莹; 潘诗敏; 李金香; 章薇; 胡莎; 严洁; 张曦] Department of Acupuncture and Moxibustion, the First Affiliated Hospital of Hunan University of CM, Changsha 410007, China;[李莹; 潘诗敏; 张曦] Graduate School of Hunan University of CM, Changsha 410208

关键词： 胃食管反流病;肝胃不和;五经配伍;调气;针刺;随机对照试验

摘要： 目的:观察五经配伍调气法针刺与常规西药治疗肝胃不和型胃食管反流病的临床疗效差异。方法:将60例患者根据西医分型分为非糜烂性反流病(NERD)和反流性食管炎(RE)两个区组,然后将两区组患者分别随机分为观察组与对照组,最终观察组28例(退出2例),对照组29例(退出1例)。在生活调护基础上,观察组用五经配伍调气法针刺治疗,穴取足三里、冲阳、胃俞、中脘、行间、少府、大敦、太冲、肝俞、期门、膻中、中庭,留针30 min,每周3次。对照组用雷贝拉唑肠溶胶囊常规口服治疗,每日1次。两组均连续治疗8周;观察两组治疗前后及治疗结束后4周临床症候积分,并从3个方面(两组主要临床症状、胃镜下炎性反应改善情况及临床总体症候)评价疗效。结果:两组治疗后及随访时症候积分均较治疗前下降(均P <0.05),随访时观察组症候积分改善优于对照组(P <0.05)。治疗后两组NERD与RE两分型患者症候积分均较治疗前下降(均P <0.05)。观察组治疗后胃脘灼热症状总有效率为82.1%(23/28),低于对照组的93.1%(27/29)(P <0.05);观察组治疗后嗳气症状总有效率为94.1%(16/17),高于对照组的75.0%(15/20)(P <0.05);观察组治疗后胃镜下炎性反应改善总有效率为75.0%(12/16),对照组为82.4%(14/17),差异无统计学意义(P >0.05);观察组治疗后临床总体症候总有效率为82.1%(23/28),对照组为86.2%(25/29),差异无统计学意义(P >0.05)。结论:五经配伍调气法针刺治疗肝胃不和型胃食管反流病能改善临床症候、胃镜下炎性反应,有改善嗳气等优势,疗效较持久。

语种： 中文

作者： 李中正;贾元斌;郭靖;王小军;宿绍敏;...

期刊： 中国针灸,2017年37(7):753-756 ISSN：0255-2930

作者机构： [阳仁达; 李中正] Graduate College of Hunan University of CM, Changsha 410208, China;[邓瑜; 贾元斌; 刘亮晶; 李中正; 刘盈盈; 郭靖; 宿绍敏; 石维坤] Medical college of Jishou University, Jishou 416000, Hunan Province, China;[王小军] Affiliated TCM Hospital of Jishou University

关键词： 湘西苗医刘氏小儿推拿;体质;因质制宜;哮喘;治未病

摘要： 湘西苗医刘氏(刘开运)小儿推拿流派是国内著名的小儿推拿流派。根据小儿五脏的生理病理特点、五行生克和苗医“五经助制”理论,灵活调整相应的“推五经”推拿调治方案,针对缓解期哮喘患儿肺禀不足质、脾禀不足质、肾禀不足质、气虚质、阴虚质、阳虚质、痰湿质、痰热质不同的体质类型,采用因质制宜的推拿方案,预防或减少哮喘复发,达到调理体质及治未病的目的。

语种： 中文

作者： Chai, Qiu-Ye;Yang, Zhen;Lin, Hou-Wen*;Han, Bing-Nan*

期刊： Marine Drugs,2016年14(11):216 ISSN：1660-3397

通讯作者： Lin, Hou-Wen;Han, Bing-Nan

作者机构： [Chai, Qiu-Ye; Lin, Hou-Wen; Han, BN; Han, Bing-Nan] Shanghai Jiao Tong Univ, State Key Lab Oncogenes & Related Genes, Res Ctr Marine Drugs, Dept Pharm,Renji Hosp,Sch Med, Shanghai 200127, Peoples R China.;[Chai, Qiu-Ye] Jiangxi Univ Tradit Chinese Med, Sch Pharm, Nanchang 330000, Peoples R China.;[Yang, Zhen] Hunan Univ Chinese Med, Grad Sch, Dept Pharm, Changsha 410208, Hunan, Peoples R China.

通讯机构： [Lin, HW; Han, BN] S;Shanghai Jiao Tong Univ, State Key Lab Oncogenes & Related Genes, Res Ctr Marine Drugs, Dept Pharm,Renji Hosp,Sch Med, Shanghai 200127, Peoples R China.

关键词： marine cyanobacteria;mollusk;alkynyl peptides;biological activity;absolute configuration

摘要： Since the 1990s, a number of terminal alkynyl residue-containing cyclic/acyclic peptides have been identified from marine organisms, especially cyanobacteria and marine mollusks. This review has presented 66 peptides, which covers over 90% marine peptides with terminal alkynyl fatty acyl units. In fact, more than 90% of these peptides described in the literature are of cyanobacterial origin. Interestingly, all the linear peptides featured with terminal alkyne were solely discovered from marine cyanobacteria. The objective of this article is to provide an overview on the types, structural characterization of these unusual terminal alkynyl fatty acyl units, as well as the sources and biological functions of their composed peptides. Many of these peptides have a variety of biological activities, including antitumor, antibacterial, antimalarial, etc. Further, we have also discussed the evident biosynthetic origin responsible for formation of terminal alkynes of natural PKS (polyketide synthase)/NRPS (nonribosome peptide synthetase) hybrids.

语种： 英文

作者： 陈其华;欧阳政洁;赵丹;王大进;李博

期刊： 中华男科学杂志,2016年22(11):1011-1015 ISSN：1009-3591

作者机构： [陈其华; 王大进] Department of Traditional Chinese Medicine Surgery, The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China;[欧阳政洁; 李博; 赵丹] Graduate School, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China

关键词： 益肾通癃胶囊;良性前列腺增生;生殖激素;小鼠

摘要： 目的:探讨益肾通癃胶囊对良性前列腺增生(BPH)模型小鼠生殖激素水平的影响。方法:对SPF雄性小鼠皮下注射丙酸睾酮5mg /(kg·d), 连续3周,待造模成功后分为6组:正常对照组,模型组,癃闭舒胶囊组,益肾通癃胶囊高、中、低剂量组,每组10只。益肾通癃胶囊高、中、低剂量组分别用1.2,0.6,0.3 g /kg益肾通癃胶囊混悬液灌胃,癃闭舒胶囊组用0.45 g /kg癃闭舒胶囊混悬液灌胃,正常对照组及模型组给予同体积蒸馏水。给药8周后处死小鼠,称取前列腺湿重并计算前列腺指数(PI), 测量小鼠血清中睾酮(T) 、雌二醇(E2)的水平、E2 /T比值,光镜下观察小鼠前列腺组织的形态变化。结果:与模型组比较,益肾通癃胶囊中、高剂量组及癃闭舒胶囊组均可降低模型小鼠前列腺湿重、PI指数(P均< 0.01) 。各组小鼠血清T、E2、E2 /T水平:正常对照组: (1.73 ± 0.02) ng /ml、(73.08 ± 1.03) pg /ml、42.30 ± 0.53;模型组: (3.86 ± 0.02) ng /ml,(145.79 ± 0.88) pg /ml,37.76 ± 0.25;癃闭舒胶囊组: (2.47 ± 0.02) ng /ml,(95.87 ± 0.47) pg /ml, 38.80 ± 0.13;益肾通癃胶囊高剂量组: (2.75 ± 0.03) ng /ml,(107.11 ± 0.61) pg /ml,38.92 ± 0.36;益肾通癃胶囊中剂量组: (2.77 ± 0.02) ng /ml,(112.16 ± 0.82) pg /ml, 40.56 ± 0.29;益肾通癃胶囊低剂量组: (2.91 ± 0.03) ng /ml,(112.68 ± 0.77) pg /ml, 38.80 ± 0.42,各组与模型组相比,差异均具有统计学意义(P < 0.05或P < 0.01) 。结论:益肾通癃胶囊可能通过降低BPH模型小鼠血清T、E2,升高E2 /T比值,揭示了益肾通癃胶囊治疗BPH的机制。

语种： 中文

作者： 曾逸笛（曾逸笛）;曹泽标;杜佳（杜佳）;陶竞杰;周小青（周小青）

期刊： 中国中西医结合杂志,2016年36(5):517-521 ISSN：1003-5370

作者机构： 湖南中医药大学, 中医诊断学湖南省重点实验室;[杜佳; 曾逸笛; 周小青; 陶竞杰] 湖南中医药大学数字中医药协同创新中心, 长沙, 410208;湖南中医药大学, 中医诊断学湖南省重点实验室, 长沙, 410208;[杜佳; 曾逸笛; 周小青; 陶竞杰] 湖南中医药大学, 中医诊断学湖南省重点实验室;[曹泽标] 湖南中医药大学, 中医诊断学湖南省重点实验室, 长沙, 410208

关键词： 中西医结合;诊疗方案;临床路径

摘要： 结合目前中西医结合现状,提出中西医结合诊疗方案可以从诊疗路径、病机及治法理论、诊疗理念等方面进行,但在结合程度上可有差异。同时具体方案的制定应根据疾病确定基本方向,并因人、因时、因地进行调整。对于制定好的结合方案,可从两者是否形成了互补、协同、激发、减毒的作用,在疗效、安全性、实用性、经济性等方面是否较既往单一的模式多解决问题,多避免不足来进行评价。

语种： 中文

作者： 毛丹;雷三林;马进安;施利;张绍钒;...

期刊： 中南大学学报(医学版),2016年41(12):1297-1304 ISSN：1672-7347

作者机构： [张绍钒; 张英进; 张四方; 施利; 毛丹] Department of Integrated Chinese and Western Medicine, Second Xiangya Hospital, Central South University, Changsha 410011, China;[雷三林] Department of General Surgery, Second Xiangya Hospital, Central South University, Changsha 410011, China;[马进安] Department of Oncology, Second Xiangya Hospital, Central South University, Changsha 410011, China;[黄建华] Hunan University of Traditional Chinese Medicine College of Pharmacy, Changsha 410028, China;[丁登峰; 刘新义] Department of Pharmacy, Second Xiangya Hospital, Central South University, Changsha 410011, China

关键词： 健脾解毒方;大肠癌;基因芯片;肿瘤血管形成;哺乳动物雷帕霉素靶蛋白;缺氧诱导因子1α;血管内皮生长因子

摘要： 目的:探讨健脾解毒方对大肠癌肿瘤血管形成相关基因表达的影响。方法:采用水提法制作健脾解毒方粗提取物,MTT法检测健脾解毒方粗提取物对大肠癌HT29细胞增殖的影响,GraphPad Prism 5软件计算IC_(50)(50% inhibitory concentration)值,Affymetrix基因表达谱芯片检测健脾解毒方干预后有显著差异表达的基因并进行路径富集分析,基于哺乳动物雷帕霉素靶蛋白(mammalian target ofrapamycin, mTOR)信号通路利用IPA(Ingenuity Pathway Analysis)软件对肿瘤血管形成的相关差异表达基因进行生物信息学分析并构建网路关系图,Western印迹法验证肿瘤血管形成相关差异表达的关键基因的蛋白表达水平。结果:健脾解毒方粗提取物能够抑制大肠癌HT29细胞增殖,处理24,48及72 h后的IC_(50)值分别为13.060, 9.646及8.448 mg/mL。Affymetrix基因表达谱芯片检测健脾解毒方干预后显著上调的基因218个,显著下调的基因252个,主要为生物合成、新陈代谢、细胞凋亡、抗原提取、血管生成等相关基因,其中肿瘤血管形成相关差异表达基因12个。IPA软件分析结果显示健脾解毒方干预后可使大肠癌中的鞘磷脂磷酸二酯酶3(sphingomyelin phosphodiesterase 3, SMPD3),VEGF,血管内皮生长因子A(vascular endothelial growth factor A,VEGFA),整合数α亚基1(integrin subunit alpha 1, ITGA1),组织蛋白酶B(cathepsin B,CTSB),组织蛋白酶S(cathepsin S,CTSS)等基因下调,GRB2相关结合蛋白2(GRB2 associated binding protein 2, GAB2), PLAUR(plasminogen activator,urokinase receptor)等基因上调。Western印迹检测显示健脾解毒方能明显下调磷酸化mTOR(phospho-mTOR, P-mTOR),信号转导和转录激活因子3(signal transducer and activator of transcription 3,STAT3),缺氧诱导因子-1α(hypoxia inducible factor-1α, HIF-1α)和VEGF蛋白表达,明显上调磷酸化P53(phoSpho-P53,P-P53)蛋白表达。结论:健脾解毒方可能通过影响mTOR-HIF-1α-VEGF信号通路抑制大肠癌肿瘤血管形成,这可能是健脾解毒方治疗大肠癌的作用机制之一。

语种： 中文

作者： 谭旭仪;高菲菲;高书图;刘又文;陈献韬;...

期刊： 中国中西医结合杂志,2016年36(1):40-43 ISSN：1003-5370

作者机构： 湖南中医药大学研究生院, 长沙, 410208;河南省洛阳正骨医院髋部损伤中心, 河南, 471000;[谭旭仪] 湖南中医药大学研究生院, 长沙, 410208;[高菲菲; 高书图; 刘又文; 陈献韬; 刘立云] 河南省洛阳正骨医院髋部损伤中心, 河南, 471000

关键词： 多孔钽棒;股骨头坏死愈胶囊;激素性股骨头坏死

摘要： 目的观察多孔钽棒配合股骨头坏死愈胶囊治疗激素性股骨头坏死的临床疗效。方法选择50例激素性股骨头坏死患者(共60髋),按照入组时间随机分为治疗组和对照组,每组各25例(30髋),对照组给予多孔钽棒植入治疗,治疗组在多孔钽棒植入术后配合股骨头坏死愈胶囊口服,5粒/次,3次/日,连续2个月,后2次/日,连续4个月,术后进行随访观察患者患髋Harris评分,评价疗效和股骨头生存时间。结果共有49例(59髋)获得随访,两组患者治疗后髋关节Harris评分较术前明显提高,差异有统计学意义(P<0.01),且治疗组治疗后Harris评分高于对照组,临床疗效及股骨头生存时间均优于对照组,差异有统计学意义(P<0.05)。结论多孔钽棒配合股骨头坏死愈胶囊治疗激素性股骨头坏死疗效较好,能显著改善患髋功能,延长股骨头生存时间。

语种： 中文

作者： 李里;潘江;陈成;章薇

期刊： 中国针灸,2016年36(1):102 ISSN：0255-2930

作者机构： 湖南中医药大学研究生院, 长沙, 410007;湖南中医药大学第一附属医院针灸推拿科, 长沙, 410007;[李里] 湖南中医药大学研究生院, 长沙, 410007;[潘江; 陈成; 章薇] 湖南中医药大学第一附属医院针灸推拿科, 长沙, 410007

关键词： 丘脑卒中;感觉异常;电针;心经;心包经

摘要： 笔者采用电针心经、心包经穴治疗丘脑卒中后感觉异常,现报道如下。

语种： 中文

作者： Wu, Lin;Feng, Xiao-Tao;Hu, Yue-Qiang;Tang, Nong*;Zhao, Qing-Shan;...

期刊： TOHOKU JOURNAL OF EXPERIMENTAL MEDICINE,2015年237(1):57-67 ISSN：0040-8727

通讯作者： Tang, Nong

作者机构： [Tang, Nong; Li, Tian-Wei; Bi, Xin-Ya; Cai, Xin-Kun; Wang, Qing-Bi; Feng, Xiao-Tao; Li, Hai-Yuan; Wu, Lin] Guangxi Univ Chinese Med, Guangxi Sci Expt Ctr Tradit Chinese Med, Nanning 530001, Guangxi, Peoples R China.;[Tang, Nong; Wu, Lin] Guangxi Univ Chinese Med, Guangxi Key Lab Chinese Med Fdn Res, Nanning 530001, Guangxi, Peoples R China.;[Tang, Nong; Hu, Yue-Qiang] Guangxi Univ Chinese Med, Dept Internal Neurol, Affiliated Hosp 1, Nanning 530001, Guangxi, Peoples R China.;[Zhao, Qing-Shan] Hunan Univ Chinese Med, Grad Sch, Changsha, Hunan, Peoples R China.;[Tang, Nong] Guangxi Univ Chinese Med, Guangxi Sci Expt Ctr Tradit Chinese Med, 179 East Mingxiu Rd, Nanning 530001, Guangxi, Peoples R China.

通讯机构： [Tang, Nong] G;Guangxi Univ Chinese Med, Guangxi Sci Expt Ctr Tradit Chinese Med, 179 East Mingxiu Rd, Nanning 530001, Guangxi, Peoples R China.

关键词： gene expression profile;hippocampus;ischemia-reperfusion;memory impairment;vascular dementia

摘要： Vascular dementia (VD) has been one of the most serious public health problems worldwide. It is well known that cerebral hypoperfusion is the key pathophysiological basis of VD, but it remains unclear how global genes in hippocampus respond to cerebral ischemia-reperfusion. In this study, we aimed to reveal the global gene expression profile in the hippocampus of VD using a rat model. VD was induced by repeated occlusion of common carotid arteries followed by reperfusion. The rats with VD were characterized by deficit of memory and cognitive function and by the histopathological changes in the hippocampus, such as a reduction in the number and the size of neurons accompanied by an increase in intercellular space. Microarray analysis of global genes displayed up-regulation of 7 probesets with genes with fold change more than 1.5 (P < 0.05) and down-regulation of 13 probesets with genes with fold change less than 0.667 (P < 0.05) in the hippocampus. Gene Ontology (GO) and pathway analysis showed that the up-regulated genes are mainly involved in oxygen binding and transport, autoimmune response and inflammation, and that the down-regulated genes are related to glucose metabolism, autoimmune response and inflammation, and other biological process, related to memory and cognitive function. Thus, the abnormally expressed genes are closely related to oxygen transport, glucose metabolism, and autoimmune response. The current findings display global gene expression profile of the hippocampus in a rat model of VD, providing new insights into the molecular pathogenesis of VD. © 2015 Tohoku University Medical Press.

语种： 英文