作者机构：
[Qiong LIU; Wei ZHU; Mailan LIU; Xia LIU; Jianan CAO; Shuning HU; Xiaorong CHANG] College of Acupuncture & Moxibustion & Tui-na, Hunan University of Chinese Medicine, Changsha 410007, China (湖南中医药大学针灸推拿学院, 长沙 410007, 中国);[Xianghong JING] Laboratory of Meridians and Collaterals, Institute of Acupuncture & Moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China (中国中医科学院针灸研究所经络研究室, 北京 100700, 中国)
通讯机构：
[Xiaorong CHANG] C;[Xianghong JING] L;Laboratory of Meridians and Collaterals, Institute of Acupuncture & Moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China (中国中医科学院针灸研究所经络研究室, 北京 100700, 中国)<&wdkj&>College of Acupuncture & Moxibustion & Tui-na, Hunan University of Chinese Medicine, Changsha 410007, China (湖南中医药大学针灸推拿学院, 长沙 410007, 中国)
摘要：
Objective: To observe the influence of acupuncture stimulus and thermal stimulus on the expression of mast cells(MCs) and 5-hydroxytryptamine(5-HT) in local acupoint area of Guanyuan (关元 CV 4) and serum 5-HT in rats. Methods: Thirty-nine male C57BL/6 rats were randomly divided into blank control group, acupuncture stimulus group and thermal stimulus group, with 13 rats in each group. The rats were stimulated by manual acupuncture at CV 4 for 5 min in acupuncture stimulus group, while those in thermal stimulus group were stimulated by adopting a thermal moxibustion apparatus on CV 4 for 30 min. The expression of MC and 5-HT in the skin in the acupoint area of CV 4 before and after acupuncture stimulus and thermal stimulus was observed and analyzed via adopting toluidine blue staining method and immunofluorescence histochemical method(5 rats were selected from each group), and the 5-HT content in serum before and after stimulus was determined through enzyme-linked immunosorbent assay(ELISA)(8 rats were selected from each group). The influence of acupuncture stimulus and thermal stimulus on the contents of MC and 5-HT in the skin and serum 5-HT in rats was analyzed and compared. Results:① After acupuncture stimulus or thermal stimulus on CV 4 of C57BL/6 rats, the number of MC in the acupoint area significantly increased when compared with that in blank group(the acupuncture stimulus group 12.40 ±2.07 vs. the blank group 3.00 ± 5.96; thermal stimulus group 26.20 ± 10.85 vs. the blank group 12.40± 2.07, both P< 0.05), and MC aggregation and degranulation were observed (the acupuncture stimulus group 17.80 ± 4.55 vs. the blank group 8.00 ± 3.16; the thermal stimulus group 24.00 ± 9.05 vs. the blank group 8.00 ± 3.16, P<0.05, P<0.01).② After acupuncture stimulus or thermal stimulus, 5-HT was released by MCs in the acupoint area, which aggregated around the blood vessels, and the number of 5-HT in the acupoint area significantly increased when compared with that in blank group (the blank group 3.00 ±1.28 vs. the acupuncture stimulus group 10.02 ± 3.21; the blank group 3.00 ± 1.28 vs. the thermal stimulus group 14.00 ± 3.94, both P < 0.01 ).③ Compared with blank group, both acupuncture stimulus and thermal stimulus could reduce the 5-HT content in serum(the blank group 0.72 ± 0.2372 vs. acupuncture stimulus group 0.43 ±0.21; the blank group 0.72 ± 0.24 vs. thermal stimulus group 0.32 ± 0.18, both P< 0.01), and the effect in thermal stimulus group was slightly superior to that in acupuncture stimulus group(P<0.05). Conclusion: Both acupuncture stimulus and thermal stimulus can cause the aggregation and degranulation of MCs and high expression of 5-HT in the acupoint area. The effect of thermal stimulus was superior to that of acupuncture stimulus in degranulation.
作者：
Liu Hong-hua;Liu Xia;Luo Hong-yan;Liu Mi;Chang Xiao-rong;...
期刊：
针灸推拿医学：英文版,2018年16(4):276-281 ISSN：1672-3597
通讯作者：
Xiao-rong Chang<&wdkj&>Mai-lan Liu
作者机构：
[Liu Hong-hua; Liu Xia; Luo Hong-yan; Liu Mi; Chang Xiao-rong; Liu Mai-lan] School of Acupuncture, Moxibustion & Tuina, Hunan University of Chinese Medicine, Changsha, 410208
通讯机构：
[Xiao-rong Chang; Mai-lan Liu] S;School of Acupuncture, Moxibustion & Tuina, Hunan University of Chinese Medicine, Changsha, China<&wdkj&>School of Acupuncture, Moxibustion & Tuina, Hunan University of Chinese Medicine, Changsha, China
关键词：
Cupping Therapy;Moxibustion Therapy;Suspended Magnetic Moxibustion Cup;Obesity;Weight Loss;Acupuncture-moxibustion Apparatus
摘要：
The suspended magnetic moxibustion cupping therapy, as a specific moxibustion method which collects moxibustion, cupping, infrared and magnetic therapy into one, and integrates with the warming and heating effect of moxibustion, sucking effect of cups, and effect of infrared light energy and magnetic energy, is a particularly unique method for weight loss. This article introduces the basic situation and mechanism of suspended magnetic cupping therapy for weight loss. For pathogenesis of deficiency, phlegm, stagnation and cold in obesity, Shuang Long Xi Zhu (two dragons playing a ball) moxibustion, abdominal Jiu Gong (nine palaces) moxibustion, San Yang Kai Tai (auspicious beginning) moxibustion and Chang Long (long dragon) moxibustion were given respectively. This therapy has inspired the clinical idea of Chinese medicine for obesity.
作者机构：
[常小荣; 刘密; 刘余; 刘迈兰; 刘红华; 田浩梅; 刘琼; 贲定严; 陈楚淘; 钟欢; 刘霞] College of Acupuncture-Moxibustion and Tuina, Hunan University of CM, Changsha 410208, China;[佘畅] Department of Acupuncture and Moxibustion, Changsha Hospital of TCM(Changsha 8th Hospital), Changsha 410002, Hunan Province
