论新形式下实现教书育人的策略
期刊：
教育教学论坛,2015年(11):91-92 ISSN：1674-9324
作者机构：
湖南中医药大学 医学院,湖南 长沙,410208;[宁毅; 赖娟; 杨胜辉; 李珊; 卢芳国] 湖南中医药大学
关键词：
教书育人;育人为本;策略
摘要：
本文结合作者的教学经历与体会,从教师牢固树立育人为本的教育思想理念、引导学生树立正确的世界观、人生观与价值观、激发学生的学习积极性与主动性、培养学生良好的竞争意识等四个方面,论新形式下实现教书育人的策略,以期抛砖引玉。
语种：
中文
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Efficient suppression of biofilm formation by a nucleic acid aptamer
作者：
Ning, Yi
（宁毅）;Cheng, Lijuan
（程莉娟）;Ling, Min;Feng, Xinru;Chen, Lingli
（陈伶利）;...
期刊：
Pathogens and Disease,2015年73(6):ftv034 ISSN：2049-632X
通讯作者：
Deng, Le
作者机构：
[Cheng, Lijuan; Ning, Yi; Ling, Min; Wu, Minxi; Feng, Xinru; Deng, Le] Hunan Normal Univ, Coll Life Sci, Dept Microbiol, Changsha 410081, Hunan, Peoples R China.;[Cheng, Lijuan; Ning, Yi; Chen, Lingli] Hunan Univ Chinese Med, Sch Med, Changsha 410208, Hunan, Peoples R China.;[Deng, Le] Hunan Normal Univ, Coll Life Sci, Dept Microbiol, Lushan Rd 36, Changsha 410081, Hunan, Peoples R China.
通讯机构：
[Deng, Le] H;Hunan Normal Univ, Coll Life Sci, Dept Microbiol, Lushan Rd 36, Changsha 410081, Hunan, Peoples R China.
关键词：
SELEX;Salmonella choleraesuis;aptamer;biofilm;biomarker;flagellin
摘要：
Biofilms are microbial communities that are attached to a solid surface using extracellular polymeric substances. Motility and initial attachment mediated by flagella are required for biofilm formation. Therefore, blocking the motility of flagella is a potential strategy to inhibit biofilm formation. In this study, single-stranded DNA aptamers specific to the Salmonella choleraesuis were selected after 14 cycles of the systematic evolution of ligands by exponential enrichment (SELEX). Among the selected aptamers, the aptamer 3 showed the highest affinity for S. choleraesuis with a dissociation constant (Kd) of 41 ± 2 nM. Aptamer 3, conjugated with magnetic beads, was then used to capture its binding target on the bacteria. After mass spectrometry and specific binding analysis, the flagellin was identified as the target captured by aptamer 3. Furthermore, inhibition experiments, inverted microscopy and atomic force microscopy demonstrated that aptamer 3 was able to control the biofilm formation and promote the inhibitory effect of an antibiotic on bacterial biofilms. Single-stranded DNA aptamers therefore have great potential as inhibitors of biofilm formation. © FEMS 2015. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.
语种：
英文
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以综合性实验为主线改革《免疫学基础与病原生物学实验》细菌学部分教学内容
期刊：
亚太教育,2015年(27):62-63 ISSN：2095-9214
作者机构：
湖南中医药大学医学院;[宁毅; 高强; 杨胜辉; 卢芳国] 湖南中医药大学
关键词：
免疫学基础与病原生物学实验;细菌学;三条主线
摘要：
针对中医类专业《免疫学基础与病原生物学实验》细菌学部分内容庞杂、分散的特点,提出了以三个综合性实验为主线,串连本课程总的细菌学实验内容的教学改革思路,一条主线为：细菌的分布、人工培养、生理生化、消毒与灭菌,穿插革兰染色实验。第二条主线为化脓性球菌培养形态特征、生化实验、血清学鉴定,穿插药敏实验,第三条主线为肠道杆菌的培养形态特征、生化鉴定、血清学鉴定,穿插抗酸染色实验。通过这三条主线,起到了对细菌学部分实验内容进行整体安排、分布,贯通细菌学实验教学的作用,达到了让学生系统掌握细菌学基础实验、并增强动手能力的目的。
语种：
中文
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