Differential expression of mRNA and miRNA in Guinea pigs following infection with HSV2v
作者：
Kuang, Lin
* （ ;Deng, Yihui
（ ;Liu, Xiaodan
（ ;Zou, Zhixiang;Mi, Lan
期刊：
EXPERIMENTAL AND THERAPEUTIC MEDICINE ,2017年14(3):2577-2583 ISSN：1792-0981
作者机构：
[Kuang, Lin; Deng, Yihui; Liu, Xiaodan] Hunan Univ Chinese Med, Key Lab Coll & Univ Hunan Prov Cytobiol & Mol Bio, 300 Xueshi Rd, Changsha 410208, Hunan, Peoples R China.;[Zou, Zhixiang] Hunan Univ Chinese Med, Hosp 1, Dept Obstet & Gynecol, Changsha 410007, Hunan, Peoples R China.;[Mi, Lan] Hunan Univ Chinese Med, Hosp 2, Dept Dermatol, Changsha 410005, Hunan, Peoples R China.
通讯机构：
[Kuang, Lin] H;Hunan Univ Chinese Med, Key Lab Coll & Univ Hunan Prov Cytobiol & Mol Bio, 300 Xueshi Rd, Changsha 410208, Hunan, Peoples R China.
关键词：
genital herpes;herpes simplex virus;micro RNA;sequence analysis;toll-like receptors
摘要：
MicroRNAs (miRNAs) are 22-nucleotide single-stranded RNAs which regulate gene expression by targeting 3' untranslated regions. Previous studies have suggested that miRNAs may be used as markers for investigating the molecular regulation of gene expression. In the present study, miRNA and mRNA expression profiles were investigated using a massively parallel next generation sequencing technique to compare herpes simplex virus (HSV)2-infected (n=3) and healthy (n=3) epithelial tissues from guinea pigs. Total RNA was isolated and RNA sequencing was performed using a HiSeq 2000 sequencing system. Differential expression of miRNA and mRNA was analyzed using two-tailed t-tests. A negative correlation was detected between the miRNAs and their predicted target genes. Following infection with HSV2, 205 and 159 miRNAs were demonstrated to be upregulated and downregulated, respectively. These differentially expressed miRNAs were associated with cellular and metabolic processes, biological regulation, response to stimuli and cellular components of the immune system, as determined by functional gene ontology analysis. Following HSV2 infection, 6 upregulated miRNAs including miR-592, miR-1245b-5p, miR-150, miR-342-5p, miR-1245b-3p and miR-124 were demonstrated to participate in the toll-like receptor (TLR) pathway by targeting related genes. These results suggested that the downregulated genes were associated with the TLR pathway after infection with HSV2. The results of reverse transcription-quantitative polymerase chain reaction analysis were consistent with RNA sequencing, indicating that the increased expression of these miRNAs downregulated the TLR pathway-associated genes, which may mediate the progression of HSV2-induced genital herpes. © 2017, Spandidos Publications. All rights reserved.
语种：
英文
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川崎病治疗进展
期刊：
现代临床医学 ,2016年42(5):328-330 ISSN：1673-1557
作者机构：
湖南中医药大学中西医结合学院,湖南长沙,410208;[匡琳; 吴俊; 李小青; 胡冲; 翟羽梦] 湖南中医药大学
关键词：
川崎病;治疗;卫气营血辨证
摘要：
川崎病（ KD）是一种病因病机不明的急性血管炎性疾病，临床上可分为典型川崎病及不典型或不完全性川崎病。由于其病因病机不明，治疗特别棘手。西医治疗川崎病基本以激素加IVIG加阿司匹林作为基本治疗方案；中医治疗主要以辨证论治，急性期以清热解毒祛邪为主，后期以滋阴养血扶正为主，中西医协同治疗在西医治疗的基础上进行卫气营血辨证治疗。由于没有特异治疗方法，大多数可发展为慢性冠脉炎，造成冠脉畸形，逐步发展为冠心病。所以寻找一种新的治疗方法迫在眉睫。
语种：
中文
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“PBL、LBL、SP”三维一体教学法在中西医结合外科学教学中的应用研究
期刊：
中国高等医学教育 ,2016年(8):99-100 ISSN：1002-1701
作者机构：
湖南中医药大学中西医结合学院,湖南长沙,410208
关键词：
LBL+PBL+SP教学法;中西医结合外科学;教学效果
摘要：
目的:探讨“PBL、LBL、SP”三维一体教学法在中西医结合外科学教学中的效果.方法:将146名本科生分为实验组与对照组,实验组采用“LBL+ PBL+ SP”教学模式,对照组以授课为主的传统教学模式.结果:实验组在激发学生学习兴趣、提高自学能力、提高合作能力、提高诊疗思维能力等教学效果方面明显优于对照组,且实验组学生的技能和实习成绩显著高于对照组(P＜0.05).结论:“PBL、LBL、SP”三维一体教学法是一种行之有效的教学模式.
语种：
中文
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Effects of a traditional Chinese medicine, Longdanxiegan formula granule, on Toll-like receptor pathway in female guinea pigs with recurrent genital herpes
作者：
Kuang, Lin
* （ ;Deng, Yihui
（ ;Liu, Xiaodan
（ ;Zou, Zhixiang;Mi, Lan
期刊：
Taiwanese Journal of Obstetrics and Gynecology ,2016年55(2):220-228 ISSN：1028-4559
作者机构：
[Deng, Yihui; Liu, Xiaodan; Kuang, Lin] Hunan Univ Chinese Med, Changsha, Hunan, Peoples R China.;[Zou, Zhixiang] Hunan Univ Chinese Med, Hosp 1, Changsha, Hunan, Peoples R China.;[Mi, Lan] Hunan Univ Chinese Med, Hosp 2, Changsha, Hunan, Peoples R China.;[Kuang, Lin] Hunan Univ Chinese Med, Key Lab Coll & Univ Hunan Prov Cytobiol & Mol Bio, Xueshi Rd 300, Changsha 410208, Hunan, Peoples R China.
通讯机构：
[Kuang, Lin] H;Hunan Univ Chinese Med, Key Lab Coll & Univ Hunan Prov Cytobiol & Mol Bio, Xueshi Rd 300, Changsha 410208, Hunan, Peoples R China.
关键词：
acyclovir;herpes simplex virus 2;Longdanxiegan formula granule;recurrent genital herpes;Toll-like receptor pathway genes
摘要：
Objective: The aim of the present study was to investigate the effects of Longdanxiegan formula granule (LDXGFG), a Chinese traditional medicine on Toll-like receptor (TLR) pathway in recurrent genital herpes. Materials and Methods: An experimental recurrent genital herpes model was constructed using herpes guinea pig model. The effect of LDXGFG on expression levels of TLR pathway genes were detected using real-time polymerase chain reaction. Furthermore, the dendritic cells and Langerhans cells were isolated and the TLR pathway genes of these cells were assayed after LDXGFG treatment. Results: The result suggested two different expression patterns of TLR pathway genes in genital herpes and recurrent genital herpes, including upregulated genes and downregulated genes. TLR1, TLR4, TLR6, TLR7, TLR8, TLR9, and TLR10 showed a significant decrease while, TLR2, TLR3, and TLR5 increased in genital herpes and recurrent genital herpes guinea pigs. Meanwhile, the downregulated genes in genital herpes and recurrent genital herpes were stimulated by LDXGFG. By contrast, the upregulated genes decreased significantly after LDXGFG treatment. In both dendritic cells and Langerhans cells, the TLR pathway genes exhibited same pattern: the LDXGFG corrected the abnormal expression of TLR pathway genes. Conclusion: The present results suggest that LDXGFG is an alternative, inexpensive, and lasting-effect medicine for herpes simplex virus 2 infection. Copyright (C) 2016, Taiwan Association of Obstetrics & Gynecology. Published by Elsevier Taiwan LLC.
语种：
英文
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丹龙止痒丸治疗血虚风燥型皮肤瘙痒症临床观察
期刊：
新中医 ,2013年45(1):67-69 ISSN：0256-7415
作者机构：
湖南中医药大学中西医结合学院,湖南长沙,410208;湖南中医药大学附属第二医院,湖南长沙,410005
关键词：
皮肤瘙痒症;丹龙止痒丸;血虚风燥
摘要：
目的：观察中药丹龙止瘁丸治疗血虚风燥型皮肤瘙痒症的临床疗效。方法：将120例患者随机分为丹龙止瘁丸组（治疗组）、乌蛇止痒丸组（对照I组）及西药芙必叮组（对照Ⅱ组）各40例．分别以相应的药物治疗，观察各组治疗前后患者瘙痒程度评分、临床证候积分，判断其近期临床疗效。结果：治疗后3组临床症状积分值均较治疗前下降（P〈0．01）；治疗组l=临床症状改善优于对照I组（P〈O．05）及对照组Ⅱ组（P〈0．01）；对照I组临床症状改善优于对照组Ⅱ组（P〈0．05）。治疗组临床疗效优于对照I组、对照Ⅱ组（P〈0．05），对照I组临床疗效优于对照组Ⅱ组（P〈0．05）。治疗组、对照I组复发率低于对照Ⅱ组（P〈0．01）。结论：丹龙止瘁丸治疗皮肤瘙痒症有确切的临床疗效。
语种：
中文
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龙胆泻肝配方颗粒对复发生殖器疱疹豚鼠Toll受体信号通路的影响
项目作者单位：
湖南中医药大学中西医结合学院
项目批准号：
81202705
资助经费：
23万
立项时间：
2013
项目类别：
青年科学基金项目
项目级别：
国家级
项目来源：
国家自然科学基金
项目关键词：
龙胆泻肝配方颗粒;生殖器疱疹豚鼠;树突状细胞;朗格汉斯细胞;Toll受体信号通路
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龙胆泻肝配方颗粒对生殖器疱疹患者外周血白细胞Toll样受体表达的影响
期刊：
湖南中医药大学学报 ,2010年30(9):165-167 ISSN：1674-070X
作者机构：
湖南中医药大学中西医结合学院,湖南,长沙,410208;深圳市三九现代中药有限公司,广东,深圳,518029;湖南师范大学生命科学院,湖南,长沙,410000;[匡琳] 湖南中医药大学(湖南省中医药研究院);[韩英先] 深圳三九现代中药有限公司
关键词：
生殖器疱疹;Toll样受体;龙胆泻肝配方颗粒;龙胆草;车前子;当归;柴胡
摘要：
目的 探讨龙胆泻肝配方颗粒对生殖器疱疹患者外周血白细胞Toll样受体(TLRs)的作用,进一步研究其抗病毒感染免疫反应的机制.方法 采用荧光定量RT-PCR的方法 检测了50例湿热型生殖器疱疹患者龙胆泻肝配方颗粒治疗前后外周血白细胞TLR1～TLR9mRNA的表达情况,并以10例正常人作为对照.结果 治疗前患者外周血TLR1～TLR9mRNA的表达水平与健康对照组比较,差异均有统计学意义(P＜0.05).其中TLR2、TLR3、TLR5较正常值高,TLR1、TLR4、TLR6、TLR7、TLR8、TLR9较正常值低.治疗后,患者血清TLR1～TLR9mRNA的表达水平均较治疗前倾向于正常.结论 龙胆泻肝配方颗粒能调节生殖器疱疹患者外周血Toll样受体的表达水平,在启动抗病毒的固有免疫反应中起着重要作用.
语种：
中文
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加味逍遥散治疗肝郁血虚型斑秃患者临床观察
期刊：
中华中医药杂志 ,2009年24(5):575-576 ISSN：1673-1727
作者机构：
[匡琳; 韩英光; 周兴] 湖南中医药大学中西医结合医院
关键词：
加味逍遥散;斑秃;临床疗效
摘要：
斑秃俗称鬼剃头,是一种突然发生的局限性斑状脱发,局部皮肤正常,无明显自觉症状,是一种常见的皮肤病.斑秃常见于青年人,无性别差异,常在不知不觉中发生头发脱落,大多数是由他人发现才知道.斑秃的治疗疗程较长,新发生长速度缓慢,患者常常心理负担较重,反而又加重病情.笔者通过临床观察以中药加味逍遥散治疗肝郁血虚型斑秃,疗效颇佳,现报道如下.
语种：
中文
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复发性生殖器疱疹中西医诊治概述
作者机构：
[匡琳; 刘翔] 湖南中医药大学中西医结合学院
会议名称：
首届赣鄂湘中西医结合(生殖内分泌)学术研讨会
会议时间：
2006-09-01
会议地点：
南昌
会议论文集名称：
首届赣鄂湘中西医结合(生殖内分泌)学术研讨会
关键词：
生殖器疱疹;中西医治疗;综述
摘要：
从中西医病因病机、中西医药治疗、中西医结合治疗方面总结了近几年来中医药治疗复发性生殖器疱疹的情况,并略述现代医学在治疗复发性生殖器疱疹现状,指出不足之处,结合中医药实验方面的研究的成果以及中医的优势,提出今后治疗复发性生殖器疱疹的方向。
语种：
中文
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