作者机构：
[林亚萍; 常小荣; 易受乡; 邹晓平; 严洁; 杨宗保] Institute of Acupuncture and Meridians, Department of Acupuncture and Moxibustion, Hunan College of Traditional Chinese Medicine, Changsha 410007 Hunan Province, China
通讯机构：
[Yan, J.] I;Institute of Acupuncture and Meridians, Department of Acupuncture and Moxibustion, Hunan College of Traditional Chinese Medicine, China
摘要：
目的:通过电针内关穴、口服复方丹参滴丸及针药结合等3种不同治疗方法对冠心病心肌缺血患者ET、CGRP进行疗效比较,找出最佳中医治疗方法,并为电针手厥阴心包经内关穴对冠心病心肌缺血具有良好的调整作用提供临床治疗依据.结果:3种不同治疗方法均能不同程度地降低ET,升高CGRP,尤以针药结合组显著,各组之间统计学有显著性差异(P＜0.05 or P＜0.01).结论:1.单纯运用电针和口服复方丹参滴丸治疗心肌缺血均有较好的疗效,但是电针与药物结合运用效果更好.2.电针手厥阴心包经内关穴对冠心病心肌缺血具有良好的调整作用.
摘要：
AIM: To explore the mechanism of the exciting effects of electro-acupuncture (EA) at Sibai on the gastric myoelectric activities. METHODS: A total of 32 rats were randomly divided into four groups. Through intraperitoneal injection with atropine (the anti-cholinergic agent by blockade of muscarinic receptors), hexamethonium (automatic nerve ganglion-blocking agent) and reserpine (anti-adrenergic agent by depleting the adrenergic nerve terminal of its norepinephrine store), effects of EA at Sibai on the gastric myoelectric activities of the denervated rats were observed. RESULTS: After intraperitoneal injection of atropine and hexamethonium, the average amplitude and ratio of period to time in the phase of high activity of gastric myoelectric slow wave, and the average numbers of the peaks of gastric myoelectric fast wave were significantly decreased (P < 0.01, P < 0.05, P < 0.01), while after intraperitoneal injection of reserpine, the aforementioned three parameters were increased (P < 0.01, P < 0.05, P < 0.01). EA at Sibai point partially relieved the inhibitory effect of atropine and hexamethonium on the gastric myoelectric activities in the rats (P < 0.05 or P > 0.05). CONCLUSION: Cholinergic and adrenergic nervous systems and autonomic nerve ganglion participate in the peripheral passage of the controlling effects of EA at Foot Yangming Channel on gastrointestinal tract. (C) 2006 The WJG Press. All rights reserved.
作者机构：
[Ai Huang; Shou-Xiang Yi; Xi-Ping Li; Ya-Ping Lin; Jie Yan; Xiao-Rong Chang; Zong-Bao Yang; Yan, J; Rong Hu] Hunan Univ Tradit Chinese Med, Inst Acupuncture & Moxibust, Changsha 410007, Peoples R China.
通讯机构：
[Jie Yan] H;Hunan Univ Tradit Chinese Med, Inst Acupuncture & Moxibust, Changsha 410007, Peoples R China.
关键词：
EA;relative particularity between Stomach Meridian of Foot-Yangming;gastric mucosal damage;stress;intestinal trefoil factor;gene expression
摘要：
AIM: To investigate electroacupunture(EA) at the acupoints of Stomach Meridian of Foot-Yangming(SMFY), Gallbladder Meridian of Foot-Yangming(SMFY) on gastric mucosal intestinal trefoil factor (ITF) gene expression detection in stress-induced rats with gastric mucosal lesion, and to explore the regulatory mechanism and significance of EA-related gastric mucosal protective effect. METHODS: Forty rats were randomly divided into 4 groups: Blank group, Model group, Model group+EA at acupoints of SMFY group("SMFY group's, and Model group+EA at acupoints of GMFY group(GMFY group). All rats (except blank group) were made model by water immersion and restraint stress (WRS). Then the gastric mucosa tissue in each rat was taken off after assessment of gastric mucosal lesion index(GUI), and the expression of ITF mRNA of the tissues was detected by reverse transcription-polymerase chain reaction(RT-PCR) method. RESULTS: Compared with Model group(54.3 +/- 1.34), the GUI value in SMFY group (31 +/- 2.21) decreased significantly(P < 0.01), so did that in GMFY group (39.8 +/- 1.62, P < 0.05), meanwhile GUI value in SMFY group was significantly lower than in GMFY group(P < 0.01). Compared with Model group (0.65 +/- 0.01), EA had a tendency to improve the expression of gastric mucosal ITFmRNA gene: such tendency existed in GMFY group (0.66 +/- 0.01) but with no signficant difference(P > 0.05), in SMFY group(0.76 +/- 0.01) with an extremely obvious difference (P < 0.01), furthermore the expression in SMFY group was significantly higher than in GMFY group (P < 0.01). CONCLUSION: The gastric mucosal protective effect by EA at the acupoints of SMFY and GMFY was related to the expression variance of ITF, indicating certain meridian specificity exists. It could be one proof for the TCM theory "Relative particularity between SMFY and stomach". (C) 2006 The WJG Press. All rights reserved.
通讯机构：
[Yan, Jie] H;Hunan Univ Tradit Chinese Med, Coll Acupuncture & Moxibust, Changsha 410007, Hunan Province, Peoples R China.
关键词：
electroacupuncture;serum;stomach meridian acupoints;gastric mucosal cells;epidermal growth Factor receptor;gene expression
摘要：
AIM: To investigate the effect of serum derived from rats treated with electroacupuncture at stomach meridian acupoints on the expression of epidermal growth factor receptor (EGFR) gene in gastric mucosal cells. METHODS: The stress-induced gastric mucosal injury in rat model was established by water-immersion and restrained stress methods. 52 rats were randomly divided into: normal group (n = 8), model group (n = 8), model serum group (n = 12), stomach serum group (n = 12), and gallbladder serum group (n = 12). The gastric mucosal cells were separated by pronase-EDTA digestion method and incubated with serum. The EGFR gene expression in gastric mucosal cells was detected by reverse transcription-polymerase chain reaction (RT-PCR) method. RESULTS: Compared with normal group (0.6860 +/- 0.0594), the serum derived from rats of the stomach group (1.2272 +/- 0.0813, P = 0.00 < 0.01) and gallbladder group (0.9640 +/- 0.0387, P = 0.00 < 0.01) had a tendency to enhance the EGFR gene expression in gastric mucosal cells. Such tendency existed in the model group (0.7104 +/- 0.0457) but with no significant difference (P = 0.495 > 0.05) and in model serum group (0.8516 +/- 0.0409) with an extremely obvious difference (P = 0.001 < 0.01). Furthermore, the EGFR gene expression in stomach serum group was significantly higher than that in gallbladder serum group (P = 0.00 < 0.01). CONCLUSION: The present study shows that serum derived from rats treated with electroacupuncture at stomach meridian acupoints can distinctly increase the EGFR gene expression of gastric mucosal cells. Therefore, there is certain meridian specificity in the serum, which could provide a proof for the TCM theory "particular relation between meridian and internal organ". (C) 2006 The WJG Press. All rights reserved.
