作者机构：
[易受乡; 严洁; 杨宗保; 常小荣; 林亚平; 邹小平] Institute of Acupuncture and Meridians, College of Acupuncture and Moxibustion, Hunan University of Traditional Chinese Medicine, Changsha 410007 Hunan Province, China
通讯机构：
[Yang, Z.-B.] I;Institute of Acupuncture and Meridians, College of Acupuncture and Moxibustion, Hunan University of Traditional Chinese Medicine, China
摘要：
AIM: To discuss the protective effect of electroacupuncture at the Foot-Yangming Meridian on gastric mucosal lesion, somatostatin (SS) and the expression of SS receptor genes (SSRimRNA) in rabbits with gastric ulcer and to further explore the relative specificity of meridians and viscera at gene expression level. METHODS: Forty rabbits were randomly divided into control group (A), gastric ulcer model group (B), Foot-Yangming Meridian group (C), Foot-Shaoyang Meridian group (D) and Foot-Taiyang Meridian group (E). The gastric ulcer model was prepared by infusing alcohol into stomach. Groups C-E were treated with electroacupuncture at points along the above meridians using meridian stimulating instruments for 7 days respectively. By the end of treatment, the index of gastric ulcer was determined, the amount of epidermal growth factor(EGF) and somatostatin was measured by radioimmunoassay (RIA). SS-R(1)mRNA expression in gastric mucosa was determined by RT-PCR. RESULTS: The value of EGF in model group was obviously lower(73.6 +/- 14.8 vs 91.3 +/- 14.9 pg/mL, P < 0.01) than that in control group. The index of gastric ulcer, content of SS and expression of SSR(1)mRNA in gastric mucosa were significantly higher than those in control group (24.88 +/- 6.29 vs 8.50 +/- 2.98 scores, P < 0.01; 2978.6 +/- 587.6 vs 1852.4 +/- 361.7 mIU/mL, P < 0.01; 2.56 +/- 0.25 vs 1.04 +/- 0.36, P < 0.01). The value of EGF in Foot-Yangming Meridian group was higher than that in model group(92.2 +/- 6.7 vs 73.6 +/- 14.8 pg/mL, P < 0.01). The index of gastric ulcer, content of SS and expression of SS-R1mRNA in gastric mucosa were significantly lower than those in control group(10.88 +/- 3.23 vs 24.88 +/- 6.29 scores, P < 0.01; 1800.2 +/- 488 vs 2978.6 +/- 587.6 mIU/mL, P < 0.01; 1.07 +/- 0.08 vs 2.56 +/- 0.25 mIU/mL, P < 0.01). Compared to the model group, the content of SS and expression of SSR(1)mRNA in gastric mucosa in Foot-Shaoyang Meridian group decreased (2441.0 +/- 488. vs 2978.6 587.6 mIU/mL, P < 0.05;1.73 +/- 0.16 vs 2.56 0.25 mIU/mL, P < 0.01). But the above parameters in Foot-Taiyang Meridian group did not improve and were significantly different from those in Foot-Yangming Meridian group (P < 0.05) CONCLUSION: Electro-acupuncture at Foot-Yangming Meridian can protect gastric mocusa against injury. The mechanism may be releted to the regulation of brain-gut peptides and the expression of SSRimRNA. (c) 2006 The WIG Press. All rights reserved.
通讯机构：
[Yan, Jie] H;Hunan Coll Tradit Chinese Med, Inst Acupuncture & Moxibust, Physiol Lab, Changsha 410007, Peoples R China.
关键词：
NTS;Firing Neuron;Gastric Distention;Meridian and Viscera;Acupuncture
摘要：
The nucleus of the solitary tract (NTS) is a primary center, not only for receiving visceral afferents, but also for somatic afferents. Previous experiments demonstrated that NTS might be a primary afferent center for connecting the Stomach Meridian (ST Medirian) and the Stomach. In this study, an extracellular recording method was used to analyze the firing neurons. The variety of NTS' neurons' responses to gastric distention (GD) and different body surface points were compared. In 52 rats, 104 firing neurons of NTS were recorded, in which there were 47 GD related neurons. For the 47 GD related neurons, there were 33 neurons responsive to acupuncture at Sibai (ST 2, belonging to ST Meridian). Therefore, in the 47 GD related neurons, the response neuron occurring rate (RNOR) for acupuncture at ST 2 was 70.21%. Meanwhile in the 47 GD related neurons, when acupuncture was performed at Quanliao (SI 18, belonging to Small Intestine Meridian) and non-acupoint respectively, there were 18 and 17 firing neurons, and the RNOR were 38.30% and 36.17% respectively. The experiment demonstrated that there were some varieties in the response of NTS neurons to GD stimuli and acupuncture at different body surface points, which suggested that the ST Meridian was more intimately linked to the Stomach and that the different acupoints do have some specificity.
通讯机构：
College of Acupuncture and Massage, Hunan University of Traditional Chinese Medicine, China
关键词：
艾灸;足三里穴;梁门穴;应激性溃疡;胃黏膜血流量;内皮素;多巴胺;细胞凋亡
摘要：
目的:探讨艾灸足三里、梁门穴对应激性溃疡胃黏膜细胞凋亡的影响,分析其与血浆多巴胺(DA)、胃黏膜内皮素(ET)的关系,揭示艾灸足三里、梁门穴对抗应激性损伤,进而保护胃黏膜的机制.方法:SD大鼠60只随机分为4组,即束缚对照组、模型组、艾灸足三里和梁门穴组、艾灸非穴点对照组,每组15只.束缚水浸应激法造模,免疫组化方法测定细胞凋亡指数(×10-6个/μm2).采用生物信号分析仪检测胃黏膜血流量(GMBF),高效液相色谱法测定血浆DA含量,放射免疫法测定胃黏膜ET含量.结果:预先艾灸足三里、梁门穴可显著降低随后的应激性胃黏膜损伤指数,降低胃黏膜ET和血浆DA含量,增加胃黏膜血流量,降低胃黏膜细胞凋亡指数.造模后,B组UI值(26.80±9.81 vs 12.00±5.94,P＜0.01)、血浆DA(9.97±3.69 μg/L vs 4.54±2.61 μg/L,P＜0.01)、胃黏膜ET(361.469±98.080 ng/L vs 149.205±94.1425 ng/L,P＜0.01)以及凋亡指数(9.65±4.19 vs 4.36±2.60,P＜0.01)显著高于A组,而GMBF低于A组(139.489±33.133 mL/min vs 377.090±85.840 mL/min,P＜0.01);C组UI值、凋亡指数显著低于B组和D组(UI:14.10±5.42 vs 26.80±9.81,26.20±7.23,P＜0.01;凋亡指数:3.00±1.58 vs 9.65±4.19,8.20±5.17,P＜0.01),而GMBF高于B组和D组(316.552±85.469 mL/min vs 139.489±33.133,141.512±58.450 mL/min,P＜0.01);C组血浆DA含量及胃黏膜ET显著低于B组(DA:4.41±2.48μg/L vs9.97±3.69 μg/L,P＜0.01;148.271±69.113 ng/L vs 361.469±98.080 ng/L,P＜0.01),但与D组比较无显著性差异(P＞0.05).结论:艾灸足三里、梁门穴预处理可减轻束缚水浸应激所造成大鼠胃黏膜的损伤程度,这一作用可能是通过降低血浆DA和胃黏膜ET含量,增加胃黏膜血流量,抑制细胞凋亡实现的.
作者机构：
[曹湘萍; 沈菁; 易受乡; 常小荣; 严洁; 林亚平; 岳增辉] College of Acupuncture and Massage, Hunan University of Traditional Chinese Medicine, Changsha 410007 Hunan Province, China
通讯机构：
[Shen, J.] C;College of Acupuncture and Massage, Hunan University of Traditional Chinese Medicine, China
通讯机构：
[Yan, Jie] H;Hunan Univ Tradit Chinese Med, Inst Acupuncture & Moxibust, Changsha 410007, Hunan, Peoples R China.
关键词：
Electroacupuncture;Gastric histology;Gastric mucosal protection;Intestinal trefoilf factor;Relative particularity between stomach meridian of foot-yangming and stomach;Stomach meridian of Foot-yangming
