通讯机构：
[Yan, Jie] H;Hunan Coll Tradit Chinese Med, Inst Acupuncture & Moxibust, Physiol Lab, Changsha 410007, Peoples R China.
关键词：
NTS;Firing Neuron;Gastric Distention;Meridian and Viscera;Acupuncture
摘要：
The nucleus of the solitary tract (NTS) is a primary center, not only for receiving visceral afferents, but also for somatic afferents. Previous experiments demonstrated that NTS might be a primary afferent center for connecting the Stomach Meridian (ST Medirian) and the Stomach. In this study, an extracellular recording method was used to analyze the firing neurons. The variety of NTS' neurons' responses to gastric distention (GD) and different body surface points were compared. In 52 rats, 104 firing neurons of NTS were recorded, in which there were 47 GD related neurons. For the 47 GD related neurons, there were 33 neurons responsive to acupuncture at Sibai (ST 2, belonging to ST Meridian). Therefore, in the 47 GD related neurons, the response neuron occurring rate (RNOR) for acupuncture at ST 2 was 70.21%. Meanwhile in the 47 GD related neurons, when acupuncture was performed at Quanliao (SI 18, belonging to Small Intestine Meridian) and non-acupoint respectively, there were 18 and 17 firing neurons, and the RNOR were 38.30% and 36.17% respectively. The experiment demonstrated that there were some varieties in the response of NTS neurons to GD stimuli and acupuncture at different body surface points, which suggested that the ST Meridian was more intimately linked to the Stomach and that the different acupoints do have some specificity.
通讯机构：
College of Acupuncture and Massage, Hunan University of Traditional Chinese Medicine, China
关键词：
艾灸;足三里穴;梁门穴;应激性溃疡;胃黏膜血流量;内皮素;多巴胺;细胞凋亡
摘要：
目的:探讨艾灸足三里、梁门穴对应激性溃疡胃黏膜细胞凋亡的影响,分析其与血浆多巴胺(DA)、胃黏膜内皮素(ET)的关系,揭示艾灸足三里、梁门穴对抗应激性损伤,进而保护胃黏膜的机制.方法:SD大鼠60只随机分为4组,即束缚对照组、模型组、艾灸足三里和梁门穴组、艾灸非穴点对照组,每组15只.束缚水浸应激法造模,免疫组化方法测定细胞凋亡指数(×10-6个/μm2).采用生物信号分析仪检测胃黏膜血流量(GMBF),高效液相色谱法测定血浆DA含量,放射免疫法测定胃黏膜ET含量.结果:预先艾灸足三里、梁门穴可显著降低随后的应激性胃黏膜损伤指数,降低胃黏膜ET和血浆DA含量,增加胃黏膜血流量,降低胃黏膜细胞凋亡指数.造模后,B组UI值(26.80±9.81 vs 12.00±5.94,P＜0.01)、血浆DA(9.97±3.69 μg/L vs 4.54±2.61 μg/L,P＜0.01)、胃黏膜ET(361.469±98.080 ng/L vs 149.205±94.1425 ng/L,P＜0.01)以及凋亡指数(9.65±4.19 vs 4.36±2.60,P＜0.01)显著高于A组,而GMBF低于A组(139.489±33.133 mL/min vs 377.090±85.840 mL/min,P＜0.01);C组UI值、凋亡指数显著低于B组和D组(UI:14.10±5.42 vs 26.80±9.81,26.20±7.23,P＜0.01;凋亡指数:3.00±1.58 vs 9.65±4.19,8.20±5.17,P＜0.01),而GMBF高于B组和D组(316.552±85.469 mL/min vs 139.489±33.133,141.512±58.450 mL/min,P＜0.01);C组血浆DA含量及胃黏膜ET显著低于B组(DA:4.41±2.48μg/L vs9.97±3.69 μg/L,P＜0.01;148.271±69.113 ng/L vs 361.469±98.080 ng/L,P＜0.01),但与D组比较无显著性差异(P＞0.05).结论:艾灸足三里、梁门穴预处理可减轻束缚水浸应激所造成大鼠胃黏膜的损伤程度,这一作用可能是通过降低血浆DA和胃黏膜ET含量,增加胃黏膜血流量,抑制细胞凋亡实现的.
作者机构：
[曹湘萍; 沈菁; 易受乡; 常小荣; 严洁; 林亚平; 岳增辉] College of Acupuncture and Massage, Hunan University of Traditional Chinese Medicine, Changsha 410007 Hunan Province, China
通讯机构：
[Shen, J.] C;College of Acupuncture and Massage, Hunan University of Traditional Chinese Medicine, China
通讯机构：
[Yan, Jie] H;Hunan Univ Tradit Chinese Med, Inst Acupuncture & Moxibust, Changsha 410007, Hunan, Peoples R China.
关键词：
Electroacupuncture;Gastric histology;Gastric mucosal protection;Intestinal trefoilf factor;Relative particularity between stomach meridian of foot-yangming and stomach;Stomach meridian of Foot-yangming
作者机构：
[邓元江] Department of Neurology, Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405 Guangdong Province, China;[林亚平; 向志勇; 吴芳; 易受乡; 郭晖; 刘卫英; 严洁] Department of Acupuncture and Massage, Hunan College of Traditional Chinese Medicine, Changsha 410007 Hunan Province, China
通讯机构：
[Yi, S.-X.] D;Department of Acupuncture and Massage, Hunan College of Traditional Chinese Medicine, China
作者机构：
[常小荣; 易受乡; 林亚平; 向志勇; 吴芳; 严洁] Department of Acupuncture and Moxibustion, Hunan College of Traditional Chinese Medicine, Changsha 410007 Hunan Province, China
通讯机构：
[Lin, Y.-P.] D;Department of Acupuncture and Moxibustion, Hunan College of Traditional Chinese Medicine, China
作者机构：
[常小荣] Department of Acupuncture & Massage, Hunan College of TCM, Changsha 410007, China
通讯机构：
Department of Acupuncture and Massage, Hunan College of TCM, China
关键词：
6-KETO-PGF1Α;TXB2
摘要：
Hyperlipemia rabbit models established with high cholesterol and fat diet were treated with direct moxibustion and medicinal cake-separated moxibustion. The post-treatment plasma 6-keto-prostaglandin F1alpha (6-keto-PGF1alpha) and thromboxane B2 (TXB2) contents were determined by radioimmunoassay. Results indicated that the plasma 6-keto-PGF1alpha content significantly increased, the TXB2 level decreased (P < 0.05) and the TXB2 /6-keto-PGF1alpha ratio also decreased (P < 0.01) in the medicinal cake-separated moxibustion group as compared with those in the model group respectively, but there was no significant difference between the medicinal cake-separated moxibustion group and the direct moxibustion group (P > 0.05), suggesting that both the medicinal cake-separated moxibustion and direct moxibustion can regulate the plasma 6-keto-PGF1alpha and TXB2 contents, and the TXB2/6-keto-PGF1alpha ratio with similar actions, and have a certain protective action on endothelial cells of the aorta in the rabbit of hyperlipemia.
