作者机构：
The Domestic First-class Discipline Construction Project of Chinese Medicine of Hunan University of Chinese Medicine, College of Acupuncture & Moxibustion and Tui-na, Hunan University of Chinese Medicine, Changsha, China;College of Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha, China;[杨建文; 林亚平; 彭艳; 张程程; 陈海交; 魏星] The Domestic First-class Discipline Construction Project of Chinese Medicine of Hunan University of Chinese Medicine, College of Acupuncture & Moxibustion and Tui-na, Hunan University of Chinese Medicine, Changsha 410000, China;[曹建中] College of Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha 410000, China
通讯机构：
[Zhang Cheng-cheng; Peng Yan] T;The Domestic First-class Discipline Construction Project of Chinese Medicine of Hunan University of Chinese Medicine, College of Acupuncture & Moxibustion and Tui-na, Hunan University of Chinese Medicine, Changsha, China<&wdkj&>The Domestic First-class Discipline Construction Project of Chinese Medicine of Hunan University of Chinese Medicine, College of Acupuncture & Moxibustion and Tui-na, Hunan University of Chinese Medicine, Changsha, China
关键词：
Acupuncture Therapy;Electroacupuncture;Calcium-activated Chloride Channel;Interstitial Cells of Cajal;Diabetes Complications;Gastroparesis;Rats
摘要：
To investigate the mechanisms of electroacupuncture (EA) at Zusanli (ST 36), Liangmen (ST 21) and Sanyinjiao (SP 6) in intervening diabetic gastroparesis (DGP) based on calcium-activated chloride channel. Forty Sprague-Dawley rats were randomly divided into four groups, including a normal control group (group A), a model group (group B), an EA group (group C) and a metoclopramide group (group D), with 10 rats in each group. A single intraperitoneal injection of 2% streptozotocin (STZ) combined with 8-week high-glucose high-fat diet was used to establish a DGP rat model. After intervention, gastrointestinal propulsive rate was observed; the expression level of transmembrane protein 16A (TMEM16A) was examined by immunohistochemistry; the Ca2+ concentration in interstitial cells of Cajal (ICCs) was detected by immunofluorescence; and whole-cell patch-clamp technique was applied to detect the current intensity of calcium-activated chloride channel (ICaCC) in ICCs in gastric antrum. After modeling, the blood glucose levels in group B, group C and group D were significantly increased compared with group A (all P<0.01); after intervention, compared with group B, the blood glucose levels in group C and group D were significantly decreased (P<0.05, P<0.01); the intra-group comparison of blood glucose level between after modeling and after intervention found significant difference only in group C (P<0.01). The gastrointestinal propulsive rates in group B, group C and group D were significantly different from that in group A (P<0.01 or P<0.05); the gastrointestinal propulsive rates were markedly higher in group C and group D than in group B (P<0.01, P<0.01). The expressions of TMEM16A in group B and group C were decreased compared with group A (P<0.01, P<0.05); the expressions of TMEM16A in group C and group D were increased compared with group B (P<0.01, P<0.05). The fluorescence intensity of Ca2+ was significantly lower in group B than in group A (P<0.01); the fluorescence intensity of Ca2+ was significantly higher in group C and group D than in group B (P<0.01, P<0.05). ICaCC in ICCs in group B was significantly decreased compared with group A; ICaCC in group C and group D were increased compared with group B. EA at Zusanli (ST 36), Liangmen (ST 21) and Sanyinjiao (SP 6) can significantly improve gastrointestinal motility in DGP rats by up-regulating the ICaCC in ICCs.
作者机构：
[Dong-Feng Z.; Xing W.; Xiao-Juan X.; Ya-Ping L.; Yan P.] The Domestic First-class Discipline Construction Project of Chinese Medicine, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China
通讯机构：
[Lin Ya-Ping] T;The Domestic First-class Discipline Construction Project of Chinese Medicine, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China
摘要：
Objective: To observe the effect of moxibustion on the mRNA and protein expressions of heat-shock protein 70 (HSP70) in gastric cancer-bearing rats. Methods: A total of 40 healthy Sprague-Dawley (SD) rats were adaptively fed for one week. The gastric cancer model was prepared by Walker-256 cancer tissue transplantation. After 7 d, 10 rats were randomly selected to verify the successful modeling, and the remaining 30 rats were divided into a model group, a moxibustion group and an infrared group by the random number table method, with 10 rats in each group. After enrollment, the moxibustion group received suspended moxibustion at Zhongwan (CV 12), Guanyuan (CV 4) and bilateral Zusanli (ST 36), (the first group of acupoints) on the 1st day, and suspended moxibustion at bilateral Pishu (BL 20) and Weishu (BL 21), (the second group of acupoints) on the 2nd day, 20 min each time, once a day. Moxibustion was alternately performed every other day at the two groups of acupoints for 21 d. From the day of enrollment, rats in the infrared group were irradiated with the infrared radiation at the stomach area on the 1st day, and at the T12-T13 interspinous region on the 2nd day, 20 min each time, once a day, and the two locations were alternately irradiated every other day for 21 d. During the treatment, rats in the model group were intervened by grasping and fixation without treatment. At the end of the treatment, blood was collected from the inner eye orbit, and the HSP70 expression in peripheral blood was determined by enzyme linked immunosorbent assay (ELISA). Rats were sacrificed, the tumor volume and growth inhibition rate were measured. The position and changes of HSP70 in gastric cancer were observed by streptavidin-perosidase (SP); HSP70 protein expression was determined by ELISA; HSP70 mRNA expression in cancer tissues was determined by reverse transcription-polymerase chain reaction (RT-PCR) assay. Results: In comparison of the model group, the volume growth of the gastric cancer in the moxibustion group was significantly restricted (P<0.01); the volume growth inhibition rate in the moxibustion group was 37.93%; the HSP70 expression in peripheral blood and the cancer tissues was significantly increased (both P<0.01); the expression of HSP70 mRNA and HSP70 content in gastric tumor were both obviously increased in the moxibustion group (P<0.01); and a large amount of HSP70 was released to the outside of cancer cells in the moxibustion group. In comparison of the model group, the volume growth of the gastric cancer in the infrared group was slightly restricted (P<0.05) with a volume growth inhibition rate of 15.89%; the HSP70 expression in the infrared group was increased significantly in peripheral blood (P<0.01) and in the gastric cancer tissues (P<0.05); more HSP70 was released outside of the cancer cells in the infrared group. In comparison of the infrared group, the volume growth of gastric cancer was more restricted in the moxibustion group (P<0.05), and the HSP70 expression in the gastric cancer tissues was also higher (P<0.05); more HSP70 was released outside of the cancer cells in the moxibustion group. Conclusion: Moxibustion and infrared treatment inhibit the gastric cancer growth in the gastric cancer-bearing rats, up-regulate the HSP70 expression in gastric cancer tissues, and promote the production and extracellular release of HSP70, and the effect of moxibustion is more obvious. Conclusion: Moxibustion and infrared treatment inhibit the gastric cancer growth in the gastric cancer-bearing rats, up-regulate the HSP70 expression in gastric cancer tissues, and promote the production and extracellular release of HSP70, and the effect of moxibustion is more obvious.
作者机构：
[Zhuojun PENG; Yupan CHEN; Huan ZHAO; Junlin SHI] School of Acupuncture-Moxibustion, Hunan University of Chinese Medicine, Changsha 410208, China (湖南中医药大学针灸推拿学院, 长沙410208, 中国);[Jing TAN; Yaping LIN] First-Class Discipline of Chinese medicine in Hunan University of Chinese Medicine, Changsha 410208, China (湖南中医药大学中医学国内一流建设学科, 长沙410208, 中国)
通讯机构：
[Jing TAN] F;First-Class Discipline of Chinese medicine in Hunan University of Chinese Medicine, Changsha 410208, China (湖南中医药大学中医学国内一流建设学科, 长沙410208, 中国)
关键词：
Moxibustion;Tumor-bearing rats with gastric cancer;Survival status;Blood glucose (GLU);Albumin (ALB);Total protein (TP);Triglyceride (TG);Viscera-free weight
摘要：
Objective: To observe the effect of moxibustion on the survival status and nutritional metabolic factors of tumor-bearing rats with gastric cancer and explore the inhibitory effect of moxibustion on the tumor-bearing rats. Methods: A total of 40 SD rats were randomly divided into a blank group, a sham-operated group, a model group and a moxibustion group, 10 rats in each group. Gastric cancer models were established in the model group and the moxibustion group. The rats of all the groups underwent the same constraining procedure for 20 min every day. Additionally, moxibustion was applied at acupoints for 20 min in the rats of the moxibustion group. There were two groups of acupoints. One group included "Zusanli (足三里ST 36)” "Zhongwan (中脘CV12) ” and "Guanyuan (关元CV4) ". The other group included bilateral "Pishu(脾俞BL 20) “ and "Weishu(胃俞BL 21) ”. Moxibustion was applied for 20 min every day at the acupoints of the two groups alternatively and lasted for 14 days. The survival status of rats was observed and the score of survival status and body weight of rats were recorded every day. At the end of intervention, the orbital blood was collected and the rats were sacrificed for sample collection. The viscera-free body weight was recorded. Using biochemical analyzer, the blood glucose (GLU), albumin (ALB), total protein (TP) and triglyceride (TG) in serum were detected. Results: (1) Regarding the score of the survival status, after modeling, the scores in the model group and the moxibustion group were all higher than those of the sham-operation group (both P < 0.05). After intervention, the score in the moxibustion group was lower than that of the model group (P < 0.05). (2) After modeling, the body weight in the moxibustion group and the model group was lower than those of the blank group, indicating a statistical significance (both P < 0.05). The body weight in the moxibustion group was higher than that of the model group after intervention, indicating the statistical significance (P < 0.05). (3) Regarding the viscera-free body weight, it was lower in the model group compared with the sham-operation group, indicating the statistical significance (P < 0.05). Compared with the model group, the viscera-free body weight was higher in the moxibustion group, indicating the statistical significance (P < 0.04). (4) Regarding the nutritional metabolic factors, compared with the sham-operation group, the levels of GLU and ALB in the model group were lower and TP was higher, presenting the statistical significance (all P < 0.05). Compared with the model group, the levels of GLU and ALB were higher in the moxibustion group and TP was lower, presenting the statistical significance (all P < 0.05). Conclusion: Moxibustion improves the survival status of tumor-bearing rats with gastric cancer, increases the body weight, the viscera-free body weight and the levels of GLU and ALB in serum and reduces TP of the tumor-bearing rats with gastric cancer and inhibits the growth of gastric cancer.
