作者机构：
[Xing W.; Ya-ping L.; Jian-wen Y.; Hai-jiao C.; Cheng-cheng Z.; Yan P.] The Domestic First-class Discipline Construction Project of Chinese Medicine of Hunan University of Chinese Medicine, College of Acupuncture & Moxibustion and Tui-na, Hunan University of Chinese Medicine, Changsha, 410000, China;[Jian-zhong C.] College of Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha, 410000, China
通讯机构：
[Zhang Cheng-cheng; Peng Yan] T;The Domestic First-class Discipline Construction Project of Chinese Medicine of Hunan University of Chinese Medicine, College of Acupuncture & Moxibustion and Tui-na, Hunan University of Chinese Medicine, Changsha, China<&wdkj&>The Domestic First-class Discipline Construction Project of Chinese Medicine of Hunan University of Chinese Medicine, College of Acupuncture & Moxibustion and Tui-na, Hunan University of Chinese Medicine, Changsha, China
关键词：
Acupuncture Therapy;Electroacupuncture;Calcium-activated Chloride Channel;Interstitial Cells of Cajal;Diabetes Complications;Gastroparesis;Rats
作者机构：
[Dong-Feng Z.; Xing W.; Xiao-Juan X.; Ya-Ping L.; Yan P.] The Domestic First-class Discipline Construction Project of Chinese Medicine, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China
通讯机构：
[Lin Ya-Ping] T;The Domestic First-class Discipline Construction Project of Chinese Medicine, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China
期刊：
Canadian Journal of Gastroenterology and Hepatology,2020年2020:1-10 ISSN：2291-2789
通讯作者：
Peng, Y
作者机构：
[Wei, Xing; Xiao, Xiaojuan; Peng, Yan; Peng, Y; Zhao, Dongfeng; Lin, Yaping] Hunan Univ Chinese Med, Coll Acupuncture & Moxibust & Tui Na, Domest First Class Discipline Construct Project C, Changsha 410000, Peoples R China.;[Chen, Qiao; Chen, Siyi] Hunan Univ Chinese Med, Sch Med, Changsha 410000, Peoples R China.
通讯机构：
[Peng, Y ] H;Hunan Univ Chinese Med, Coll Acupuncture & Moxibust & Tui Na, Domest First Class Discipline Construct Project C, Changsha 410000, Peoples R China.
摘要：
<jats:p><jats:italic>Background</jats:italic>. The incidence of diabetic gastroparesis (DGP) is mainly blamed to abnormity of interstitial cells of Cajal (ICCs). Autophagy could degrade damaged proteins and organelles to keep intracellular homeostasis, and it could directly influence structure and number of cells. In this study, we aimed to figure out the relationship between DGP and autophagy of ICCs. <jats:italic>Methods</jats:italic>. Sixty Sprague-Dawley (SD) rats were randomly divided into normal control group (NC, 10) and modeling group (50). Rats in the modeling group were injected 2% streptozotocin (STZ) and fed with high-glucose and high-fat diet for 8 weeks in order to establish DGP rat model. After modeling, 30 successfully modeled rats were randomly selected and separated into diabetic gastroparesis group (DGP, 10), GDP rats with electroacupuncture group (EA, 10), and GDP rats with metoclopramide group (MP, 10). When the intervention was completed, blood glucose was measured by ONE TOUCH glucometer and gastrointestinal propulsive rate was detected through measuring optical density. Autophagosomes were observed under transmission electron microscope (TEM). The expression of LC3 protein and P62 protein was measured by Western blot. When ICCs were transfected with GFP-RFP-LC3 plasmid, autophagy flux was observed by laser scanning confocal microscope. <jats:italic>Results</jats:italic>. (1) After intervention, compared with blood glucose of rats in the NC group, all of the DGP, EA, and MP groups were remarkably increased (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M1"><mml:mrow><mml:mi>P</mml:mi><mml:mo><</mml:mo><mml:mn>0.01</mml:mn></mml:mrow></mml:math>); compared with the DGP group, the blood glucose of the EA and MP groups was decreased greatly (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M2"><mml:mrow><mml:mi>P</mml:mi><mml:mo><</mml:mo><mml:mn>0.01</mml:mn></mml:mrow></mml:math>). (2) Compared with gastrointestinal propulsive rate of rats in the NC group, no matter gastric emptying rate or intestinal propulsive rate, the EA and MP groups were significantly reduced (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M3"><mml:mrow><mml:mi>P</mml:mi><mml:mo><</mml:mo><mml:mn>0.01</mml:mn></mml:mrow></mml:math>); compared with the NC group, gastric emptying rate and intestinal propulsive rate in the EA group were obviously decreased (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M4"><mml:mrow><mml:mi>P</mml:mi><mml:mo><</mml:mo><mml:mn>0.05</mml:mn></mml:mrow></mml:math>, <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M5"><mml:mrow><mml:mi>P</mml:mi><mml:mo><</mml:mo><mml:mn>0.01</mml:mn></mml:mrow></mml:math>); compared with the DGP group, the EA and MP groups were increased significantly (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M6"><mml:mrow><mml:mi>P</mml:mi><mml:mo><</mml:mo><mml:mn>0.01</mml:mn></mml:mrow></mml:math>). (3) Compared with the NC group, intensity of RFP and GFP in the DGP group was obviously increased (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M7"><mml:mrow><mml:mi>P</mml:mi><mml:mo><</mml:mo><mml:mn>0.05</mml:mn></mml:mrow></mml:math>, <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M8"><mml:mrow><mml:mi>P</mml:mi><mml:mo><</mml:mo><mml:mn>0.01</mml:mn></mml:mrow></mml:math>), in other words, the DGP group accompanying suppression of autophagy; compared with the DGP group, intensity of RFP and GFP in the EA group was decreased significantly (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M9"><mml:mrow><mml:mi>P</mml:mi><mml:mo><</mml:mo><mml:mn>0.05</mml:mn></mml:mrow></mml:math>, <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M10"><mml:mrow><mml:mi>P</mml:mi><mml:mo><</mml:mo><mml:mn>0.01</mml:mn></mml:mrow></mml:math>). (4) There was no autophagosome in the NC group, and an autophagosome existed in the DGP group. Both EA and MP groups found autophagy. (5) When coming to LC3 II/LC3 I, compared with the NC group, the ratio was enhanced in the DGP and EA groups (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M11"><mml:mrow><mml:mi>P</mml:mi><mml:mo><</mml:mo><mml:mn>0.01</mml:mn></mml:mrow></mml:math>, <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M12"><mml:mrow><mml:mi>P</mml:mi><mml:mo><</mml:mo><mml:mn>0.05</mml:mn></mml:mrow></mml:math>); compared with the DGP group, LC3 II/LC3 I was dramatically decreased in the MP and EA groups (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M13"><mml:mrow><mml:mi>P</mml:mi><mml:mo><</mml:mo><mml:mn>0.01</mml:mn></mml:mrow></mml:math>). (6) As the substrate of degradation, the expression of P62 in the other three groups was significantly increased (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M14"><mml:mrow><mml:mi>P</mml:mi><mml:mo><</mml:mo><mml:mn>0.01</mml:mn></mml:mrow></mml:math>) compared with the NC group; compared with the DGP group, the amount of P62 in the EA and MP groups was reduced greatly (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M15"><mml:mrow><mml:mi>P</mml:mi><mml:mo><</mml:mo><mml:mn>0.01</mml:mn></mml:mrow></mml:math>). <jats:italic>Conclusion</jats:italic>. The impaired autophagy flux in ICCs is the pathological basis of diabetic gastroparesis, blaming to fusion dysfunction of autophagosome and lysosome and electroacupuncture (EA) could ease the suppression of autophagy to improve gastric motility.</jats:p>
作者机构：
[Zhuojun PENG; Yupan CHEN; Huan ZHAO; Junlin SHI] School of Acupuncture-Moxibustion, Hunan University of Chinese Medicine, Changsha 410208, China (湖南中医药大学针灸推拿学院, 长沙410208, 中国);[Jing TAN; Yaping LIN] First-Class Discipline of Chinese medicine in Hunan University of Chinese Medicine, Changsha 410208, China (湖南中医药大学中医学国内一流建设学科, 长沙410208, 中国)
通讯机构：
[Jing TAN] F;First-Class Discipline of Chinese medicine in Hunan University of Chinese Medicine, Changsha 410208, China (湖南中医药大学中医学国内一流建设学科, 长沙410208, 中国)
关键词：
Moxibustion;Tumor-bearing rats with gastric cancer;Survival status;Blood glucose (GLU);Albumin (ALB);Total protein (TP);Triglyceride (TG);Viscera-free weight
摘要：
Objective: To observe the effect of moxibustion on the survival status and nutritional metabolic factors of tumor-bearing rats with gastric cancer and explore the inhibitory effect of moxibustion on the tumor-bearing rats. Methods: A total of 40 SD rats were randomly divided into a blank group, a sham-operated group, a model group and a moxibustion group, 10 rats in each group. Gastric cancer models were established in the model group and the moxibustion group. The rats of all the groups underwent the same constraining procedure for 20 min every day. Additionally, moxibustion was applied at acupoints for 20 min in the rats of the moxibustion group. There were two groups of acupoints. One group included "Zusanli (足三里ST 36)” "Zhongwan (中脘CV12) ” and "Guanyuan (关元CV4) ". The other group included bilateral "Pishu(脾俞BL 20) “ and "Weishu(胃俞BL 21) ”. Moxibustion was applied for 20 min every day at the acupoints of the two groups alternatively and lasted for 14 days. The survival status of rats was observed and the score of survival status and body weight of rats were recorded every day. At the end of intervention, the orbital blood was collected and the rats were sacrificed for sample collection. The viscera-free body weight was recorded. Using biochemical analyzer, the blood glucose (GLU), albumin (ALB), total protein (TP) and triglyceride (TG) in serum were detected. Results: (1) Regarding the score of the survival status, after modeling, the scores in the model group and the moxibustion group were all higher than those of the sham-operation group (both P < 0.05). After intervention, the score in the moxibustion group was lower than that of the model group (P < 0.05). (2) After modeling, the body weight in the moxibustion group and the model group was lower than those of the blank group, indicating a statistical significance (both P < 0.05). The body weight in the moxibustion group was higher than that of the model group after intervention, indicating the statistical significance (P < 0.05). (3) Regarding the viscera-free body weight, it was lower in the model group compared with the sham-operation group, indicating the statistical significance (P < 0.05). Compared with the model group, the viscera-free body weight was higher in the moxibustion group, indicating the statistical significance (P < 0.04). (4) Regarding the nutritional metabolic factors, compared with the sham-operation group, the levels of GLU and ALB in the model group were lower and TP was higher, presenting the statistical significance (all P < 0.05). Compared with the model group, the levels of GLU and ALB were higher in the moxibustion group and TP was lower, presenting the statistical significance (all P < 0.05). Conclusion: Moxibustion improves the survival status of tumor-bearing rats with gastric cancer, increases the body weight, the viscera-free body weight and the levels of GLU and ALB in serum and reduces TP of the tumor-bearing rats with gastric cancer and inhibits the growth of gastric cancer.
