期刊：
International Journal of Molecular Medicine,2017年40(4):1285-1293 ISSN：1107-3756
通讯作者：
Kwon, Dong-Yeul;Liu, Xiang-Qian
作者机构：
[Zhou, Tian; Kong, Ryong; Kang, Ok-Hwa; Kwon, Dong-Yeul; Seo, Yun-Soo; Mun, Su-Hyun] Wonkwang Univ, Dept Oriental Pharm, Coll Pharm, Iksan 570749, Jeonbuk, South Korea.;[Zhou, Tian; Kong, Ryong; Kang, Ok-Hwa; Kwon, Dong-Yeul; Seo, Yun-Soo; Mun, Su-Hyun] Wonkwang Univ, Wonkwang Oriental Med Res Inst, Inst Biotechnol, Iksan 570749, Jeonbuk, South Korea.;[Li, Zhi; Liu, Xiang-Qian] Hunan Univ Chinese Med, Sch Pharm, 300 Xueshi Rd, Changsha 410208, Hunan, Peoples R China.;[Shin, Dong-Won] Sunchon Natl Univ, Dept Oriental Med Resources, Coll Bio Ind Sci, Sunchon 540742, Jeonnam, South Korea.
通讯机构：
[Kwon, Dong-Yeul] W;[Liu, Xiang-Qian] H;Wonkwang Univ, Dept Oriental Pharm, Coll Pharm, Iksan 570749, Jeonbuk, South Korea.;Wonkwang Univ, Wonkwang Oriental Med Res Inst, Inst Biotechnol, Iksan 570749, Jeonbuk, South Korea.;Hunan Univ Chinese Med, Sch Pharm, 300 Xueshi Rd, Changsha 410208, Hunan, Peoples R China.
关键词：
ursolic acid 3-O-alpha-L-arabinopyranoside;oxacillin;methicillin-resistant Staphylococcus aureus;synergism;detergent;ATPase inhibitor;producing penicillin-binding protein 2a
摘要：
The objective of the present study was to investigate the antibacterial activity of a single constituent, ursolic acid 3-O-alpha-L-arabinopyranoside (URS), isolated from the leaves of Acanthopanax henryi (Oliv.) Harms, alone and in combination with oxacillin (OXA) against methicillin-resistant Staphylococcus aureus (MRSA). A broth microdilution assay was used to determine the minimal inhibitory concentration (MIC). The synergistic effects of URS and OXA were determined using a checkerboard dilution test and time-kill curve assay. The mechanism of action of URS against MRSA was analyzed using a viability assay in the presence of a detergent and an ATPase inhibitor. Morphological changes in the URS-treated MRSA strains were evaluated via transmission electron microscopy (TEM). In addition, the producing penicillin-binding protein 2a (PBP2a) protein level was analyzed using western blotting. The MIC value of URS against MRSA was found to be 6.25 microg/ml and there was a partial synergistic effect between OXA and URS. The time-kill growth curves were suppressed by OXA combined with URS at a sub-inhibitory level. Compared to the optical density at 600 nm (OD600) value of URS alone (0.09 microg/ml), the OD600 values of the suspension in the presence of 0.09 microg/ml URS and 0.00001% Triton X-100 or 250 microg/ml N,N'-dicyclohexylcarbodiimide reduced by 56.6 and 85.9%, respectively. The TEM images of MRSA indicated damage to the cell wall, broken cell membranes and cell lysis following treatment with URS and OXA. Finally, an inhibitory effect on the expression of PBP2a protein was observed when cells were treated with URS and OXA compared with untreated controls. The present study suggested that URS was significantly active against MRSA infections and revealed the potential of URS as an effective natural antibiotic.
期刊：
Korean Journal of Pharmacognosy (생약학회지),2017年48(2):108-112 ISSN：0253-3073
通讯作者：
Kim, Y.-C.
作者机构：
Institute of Pharmaceutical Research and Development, College of Pharmacy, Wonkwang University, Iksan, 54538, South Korea;School of Pharmacy, Hunan University of Chinese Medicine, Changsha, 410208, China
通讯机构：
Institute of Pharmaceutical Research and Development, College of Pharmacy, Wonkwang University, Iksan, South Korea
作者机构：
[Liu, Zu Zhen] Hunan Society of Chinese Medicine;[Kim, Kwan-Woo; Kim, Youn-Chul] College of Pharmacy, Wonkwang University;[Yook, Chang Soo] School of Pharmacy, KyungHee University;[Li, Xiao Jun; Wang, Xiang; Li, Zhi; Liu, Xiang Qian] School of Pharmacy, Hunan University of Chinese Medicine
通讯机构：
School of Pharmacy, Hunan University of Chinese Medicine, Changsha, Hunan, China
作者机构：
School of Pharmacy,Hunan University of Chinese Medicine, Changsha, 410208;College of Life Science,Zhejiang Sci-Tech University, Hangzhou, 310018;College of Pharmacy,Kyung-Hee University, Korea, Seoul, 130-701;[王翔; 李小军; 李芝; 刘向前] School of Pharmacy,Hunan University of Chinese Medicine, Changsha, 410208;[张晓丹] College of Life Science,Zhejiang Sci-Tech University, Hangzhou, 310018
期刊：
Korean Journal of Pharmacognosy (생약학회지),2016年47(1):7-11 ISSN：0253-3073
通讯作者：
Oh, H.
作者机构：
Institute of Pharmaceutical Research and Development, College of Pharmacy, Wonkwang University, Iksan, 54538, South Korea;School of Pharmacy, Hunan University of Chinese Medicine, Changsha, 410208, China;Natural Botanical Garden, Wonkwang University, Iksan, 54538, South Korea;Hanbang Body-Fluid Research Center, Wonkwang University, Iksan, 54538, South Korea
通讯机构：
Institute of Pharmaceutical Research and Development, College of Pharmacy, Wonkwang University, Iksan, South Korea
期刊：
Molecular Medicine Reports,2016年14(4):3749-3758 ISSN：1791-2997
通讯作者：
Kwon, Dong-Yeul;Liu, Xiangqian
作者机构：
[Tian, Zhou; Kang, Ok-Hwa; Kwon, Dong-Yeul; Kang, Da-Hye; Seo, Yun-Soo] Wonkwang Univ, Coll Pharm, Dept Oriental Pharm, 460 Iksan Daero, Iksan 570749, Jeonbuk, South Korea.;[Tian, Zhou; Kang, Ok-Hwa; Kwon, Dong-Yeul; Kang, Da-Hye; Seo, Yun-Soo] Wonkwang Univ, Wonkwang Oriental Med Res Inst, Inst Biotechnol, 460 Iksan Daero, Iksan 570749, Jeonbuk, South Korea.;[Liu, Xiangqian; Li, Zhi] Hunan Univ Chinese Med, Sch Pharm, 1 Xiangzui, Changsha 410208, Hunan, Peoples R China.;[Kong, Ryong; Mun, Su-Hyun] Wonkwang Univ, Profess Grad Sch Oriental Med, Coll Oriental Med, Plus Team BK21, Iksan 570749, Jeonbuk, South Korea.
通讯机构：
[Kwon, Dong-Yeul] W;[Liu, Xiangqian] H;Wonkwang Univ, Coll Pharm, Dept Oriental Pharm, 460 Iksan Daero, Iksan 570749, Jeonbuk, South Korea.;Wonkwang Univ, Wonkwang Oriental Med Res Inst, Inst Biotechnol, 460 Iksan Daero, Iksan 570749, Jeonbuk, South Korea.;Hunan Univ Chinese Med, Sch Pharm, 1 Xiangzui, Changsha 410208, Hunan, Peoples R China.
关键词：
Ciwujianoside C3;Acanthopanax henryi (Oliv.) Harms;MAPKs;proinflammatory cytokines;TLR4;NF-kappa B
摘要：
The present study aimed to investigate the unknown mechanisms underlying the antiinflammatory activity of Ciwujianoside C3 (CJS C3), extracted from the leaves of Acanthopanax henryi Harms, on lipopolysaccharide (LPS)stimulated RAW 264.7 cells. Cells were treated with CJS C3 for 1 h prior to the addition of 200 ng/ml LPS. Cell viability was measured using the MTS assay. Nitric oxide levels were determined by Griess assay. Proinflammatory cytokine production was measured by enzymelinked immunosorbent assay. The expression levels of cyclooxygenase (COX)2, inducible nitric oxide synthase (iNOS), and mitogenactivated protein kinases (MAPKs) were investigated by western blotting, reverse transcription (RT)polymerase chain reaction (PCR) and RTquantitative PCR. Nuclear factor (NF)kappaB/p65 localization, and interaction of the TLR4 receptor with LPS was examined by immunofluorescence assay. The results indicated that CJS C3 exhibited no cytotoxicity at the measured concentrations. Treatment with CJS C3 inhibited NO production, proinflammatory cytokine levels, including interleukin (IL)6, tumor necrosis factor (TNF)alpha, and prostaglandin E2 (PGE2), and protein and mRNA expression levels of iNOS and COX2. Furthermore, CJS C3 suppressed phosphorylation of extracellular signalregulated kinases and cjun Nterminal kinases. It was also able to suppress activation of NFkappaB via inhibition of the TLR4 signaling pathway. These results suggested that CJS C3 exerts inhibitory effects on LPSinduced PGE2, NO, IL6 and TNFalpha production. In addition, iNOS and COX2 expression was decreased in murine macrophages. These inhibitory effects may be achieved via suppression of MAPKs and NFkappaB phosphorylation following inhibition of the TLR4 signaling pathway.
期刊：
Journal of Chromatographic Science,2016年54(4):574-582 ISSN：0021-9665
通讯作者：
Zhang, Xiaodan
作者机构：
[Zhang, Xiaodan; Li, Xiaolong] Zhejiang Sci Tech Univ, Coll Life Sci, Hangzhou, Zhejiang, Peoples R China.;[Zhou, Xinqi] Focused Photon Hangzhou Inc, Hangzhou, Zhejiang, Peoples R China.;[Liu, Xiangqian] Hunan Univ Chinese Med, Sch Pharm, Changsha, Hunan, Peoples R China.;[Whang, Wankyunn] Chung Ang Univ, Pharmaceut Resources Bot Lab, Seoul, South Korea.
通讯机构：
[Zhang, Xiaodan] Z;Zhejiang Sci Tech Univ, Coll Life Sci, Hangzhou, Zhejiang, Peoples R China.
摘要：
Acanthopanax species are used in traditional medicine to treat numerous diseases. A simultaneous high-performance liquid chromatography (HPLC) method was developed and validated for the determination of the flavonoid and phenolic content of Acanthopanax leaves. HPLC analysis was performed on an AKZO NOBEL Kromasil 100-5C18 column (250 × 4.6 mm, 5 µm) using a gradient elution of acetonitrile–water containing 0.2% formic acid with a flow rate of 1.0 mL/min, monitored at 320 nm. The method was linear over the range 1–500 µg/mL (determination coefficients R2 > 0.999). Satisfactory intraday and interday precision was achieved, with the relative standard deviation (RSD) <2.99%. The mean recoveries measured at the three concentrations were in the range of 90.11–104.83%, with RSD <2.91% for the targets. Twenty-four samples of Acanthopanax leaves from different species and locations were examined using this analytical method, and their chemical profiles provided information for the chemotaxonomic investigation. The established method is simple, rapid and reliable for the quality control of Acanthopanax leaves of various species from different collections. The complete phenolic and flavonoid profiles of Acanthopanax leaves of various species have been established.
摘要：
目的：采用高效液相色谱法建立湘葛一号根指纹图谱，并同时测定5种异黄酮的含量。方法色谱条件：采用 AT · LI‐CHROM C18色谱柱（250 mm ×4．6 mm ，5μm）；以甲醇‐水溶液为流动相进行梯度洗脱；流速为1．0 mL · min－1；检测波长为250 nm ；柱温为30℃。对不同采收期的10批样品的图谱进行测定，运用中国药典委员会的“中药色谱指纹图谱相似度评价软件2004AB”进行评价，并对5种异黄酮成分进行含量测定。结果建立了湘葛一号根 HPLC指纹图谱，各色谱峰分离度良好，确定了7个共有峰，并采用对照品指认了其中5个色谱峰。含量测定条件通过方法学验证，葛根素、大豆苷、染料木苷、大豆苷元、染料木素的线性范围分别为5．00～50．00，1．00～10．00，1．82～18．20，1．00～10．00和2．00～20．00μg · mL－1，线性关系良好（r＞0．999），平均加样回收率为98．8％～101．8％，5种异黄酮含量测定结果可初步确定适宜采收期为12月份。结论该方法所建立的湘葛一号根指纹图谱特征性强、方法简单、结果可靠，结合主要有效成分含量测定可更好地用于其质量控制，对其鉴定也具有参考价值。
作者机构：
School of Pharmacy, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;Broad-Ocean Bio-Science and Technique Co., Ltd of Changsha, Changsha, Hunan 410205, China;College of Life Science, Zhejiang Sci-Tech University, Hangzhou, Zhejiang 310018, China
通讯机构：
School of Pharmacy, Hunan University of Chinese Medicine, Changsha, Hunan, China
关键词：
Acanthopanax gracilistylus W. W. Smith;Anti-inflammatory activity;Cytotoxicity;HPLC fingerprinting;Lupane-triterpenoids
作者机构：
[刘喜舞] Department of Pharmacy, Hospital of Hunan Armed Police Corps, Changsha, 410006, China;[郑礼胜] Tianjin Institute of Pharmaceutical Research, Tianjin, 300193, China;[刘向前; 谢霞] School of Pharmacy, Hunan University of Chinese Medicine, Changsha, 410208, China;[戴玲] Changsha Broad-Ocean Bio-Science and Technique Co. Ltd., Changsha, 410205, China
通讯机构：
[Liu, X.-Q.] S;School of Pharmacy, Hunan University of Chinese Medicine, Changsha, China
作者机构：
School of Pharmacy, Hunan University of Chinese Medicine, Changsha, 410208, China;Broad-Ocean Bio-Science and Technique Co., Ltd of Changsha, Changsha, Hunan 410205, China;Korea Research Institute of Bioscience and Biotechnology, Chungbuk, 363-883, South Korea
通讯机构：
School of Pharmacy, Hunan University of Chinese Medicine, Changsha, China
