作者： Wang, S.;Luo, J.;Liu, X. -Q.;Kang, O. -H.*;Kwon, D. -Y.

期刊： Letters in Applied Microbiology,2021年72(3):238-244 ISSN：0266-8254

通讯作者： Kang, O. -H.;Kwon, DY

作者机构： [Kwon, DY; Wang, S.; Kang, O. -H.; Kwon, D. -Y.] Wonkwang Univ, Dept Oriental Pharm, Coll Pharm, Iksan 54538, Jeonbuk, South Korea.;[Kwon, DY; Wang, S.; Kang, O. -H.; Kwon, D. -Y.] Wonkwang Univ, Wonkwang Oriental Med Res Inst, Iksan 54538, Jeonbuk, South Korea.;[Liu, X. -Q.; Luo, J.] Hunan Univ Chinese Med, Sch Pharm, Changsha, Hunan, Peoples R China.

通讯机构： [Kwon, DY ; Kang, OH] W;Wonkwang Univ, Dept Oriental Pharm, Coll Pharm, Iksan 54538, Jeonbuk, South Korea.;Wonkwang Univ, Wonkwang Oriental Med Res Inst, Iksan 54538, Jeonbuk, South Korea.

关键词： Bacteria;Gene expression;Proteins;Strain;Anti-bacterial activity;Meca;Methicillin-resistant staphylococcus aureus;Penicillin- binding proteins;Penicillin-binding protein 2a;Reference strains;S. aureus;Sanguisorbigenin;Synergy;β-lactam antibiotics;Antibiotics;ampicillin;antiinfective agent;oxacillin;penicillin binding protein 2a;sanguisorbigenin;triterpene;unclassified drug;antiinfective agent;bacterial protein;mecA protein, Staphylococcus aureus;penicillin binding protein;plant medicinal product;antibiotics;antimicrobial activity;concentration (composition);gene expression;polymerase chain reaction;protein;root system;antibacterial activity;Article;bacterial gene;bacterial strain;controlled study;drug efficacy;drug mechanism;drug potency;drug potentiation;drug structure;gene expression;high performance liquid chromatography;mecA gene;methicillin resistant Staphylococcus aureus;minimum inhibitory concentration;nonhuman;plant root;protein expression;real time polymerase chain reaction;Sanguisorba officinalis;Western blotting;chemistry;drug effect;human;metabolism;methicillin resistant Staphylococcus aureus;microbial sensitivity test;Sanguisorba;methicillin resistant Staphylococcus aureus;Sanguisorba officinalis;Staphylococcus aureus;Anti-Bacterial Agents;Bacterial Proteins;Humans;Methicillin-Resistant Staphylococcus aureus;Microbial Sensitivity Tests;Penicillin-Binding Proteins;Plant Preparations;Plant Roots;Sanguisorba

摘要： The present study evaluated the antibacterial activity and the synergy of the sanguisorbigenin (SGB) from the dried root of Sanguisorba officinalis L. combined with β-lactam antibiotics against methicillin-resistant Staphylococcus aureus. A total of six strains of reference strain and clinical isolates were used to determine the antibacterial activity using a broth microdilution assay, and the synergistic effects were determined using a checkerboard assay. To analyse the mechanism of synergy, we conducted the level of penicillin-binding protein 2a by western blot. In addition, quantitative RT-PCR was performed to analyse the mecA gene expression. The minimal inhibitory concentration values of SGB against six strains of S. aureus were in the range of 12·5–50μgml−1, and there were synergy, or partial synergy effects when SGB was combined with antibiotics. Furthermore, when treated with SGB, the level of penicillin-binding protein 2a and the expression of the mecA gene was reduced significantly. In conclusion, this study demonstrated that SGB is a potential natural antibacterial agent against methicillin-resistant S. aureus that represents a considerable burden on the healthcare system worldwide, and may an exceptionally modulator of β-lactam antibiotics. © 2020 The Authors. published by John Wiley & Sons Ltd on behalf of Society for Applied Microbiology.

语种： 英文

作者： 肖瑾;肖珊;罗姣;鲁曼霞;卢茂芳;...

期刊： 中草药,2021年52(06):1834-1843 ISSN：0253-2670

通讯作者： Liu, X.-Q.

作者机构： [肖瑾; 卢茂芳; 肖珊; 罗姣; 鲁曼霞; 刘向前] School of Pharmacy, Hunan University of Chinese Medicine, Changsha, 410208, China

通讯机构： [Liu, X.-Q.] S;School of Pharmacy, China

关键词： 4-裂环羽扇豆烷型三萜;生物活性;化学成分;药理作用;五加属;抗炎;抗肿瘤

摘要： 3,4-裂环羽扇豆烷型三萜是五环三萜类化合物,在五加属植物中含量较为丰富。药理学研究表明该类成分具有广泛的生物活性,主要包括抗炎、抗菌、抗肿瘤等作用,具有潜在开发应用价值。通过查阅国内外相关文献报道,从化学结构特征、植物来源和生物活性等方面对天然来源的3,4-裂环羽扇豆烷型三萜类化合物的研究进展进行综述,以期为此类化合物的进一步研究及五加属植物药用资源的合理应用与开发提供参考依据。

语种： 中文

作者： Li, Q. -Q.;Luo, J.;Liu, X. -Q.;Kwon, D. -Y.*;Kang, O. -H.

期刊： Letters in Applied Microbiology,2021年72(6):669-676 ISSN：0266-8254

通讯作者： Kwon, D. -Y.;Kang, OH

作者机构： [Li, Q. -Q.; Kwon, D. -Y.; Kang, O. -H.; Kwon, DY] Wonkwang Univ, Coll Pharm, Dept Oriental Pharm, Jeonbuk, South Korea.;[Li, Q. -Q.; Kwon, D. -Y.; Kang, O. -H.; Kwon, DY] Wonkwang Univ, Wonkwang Oriental Medicines Res Inst, Jeonbuk, South Korea.;[Liu, X. -Q.; Luo, J.] Hunan Univ Chinese Med, Sch Pharm, Changsha, Hunan, Peoples R China.

通讯机构： [Kwon, DY; Kang, OH ] W;Wonkwang Univ, Coll Pharm, Dept Oriental Pharm, Jeonbuk, South Korea.;Wonkwang Univ, Wonkwang Oriental Medicines Res Inst, Jeonbuk, South Korea.

关键词： Electrophoresis;Medicine;Microwave integrated circuits;Proteins;Acanthopanax;Acanthopanax henryi(oliv.) harm;Anti-bacterial activity;Eleutheroside K;Eleutherosides;Methicillin;Methicillin-resistant staphylococcus aureus;Penicillin- binding proteins;Penicillin-binding protein 2a;Synergistic effect;Bacteria;ABC transporter;ampicillin;antibiotic agent;beta lactam antibiotic;ciprofloxacin;eleutheroside k;oxacillin;penicillin binding protein 2a;plant extract;unclassified drug;antiinfective agent;dicyclohexylcarbodiimide;eleutherosides;octoxinol;oxacillin;penicillin binding protein;plant extract;antibiotic resistance;bacterium;bioassay;concentration (composition);gene expression;isolated population;traditional medicine;Acanthopanax;Acanthopanax henryi;antibacterial activity;antibiotic resistance;Article;bacteriostatic activity;broth dilution;column chromatography;controlled study;fractional inhibitory concentration index;high performance liquid chromatography;inhibitory concentration;mass spectrometry;membrane permeability;methicillin resistance;methicillin resistant Staphylococcus aureus infection;minimum inhibitory concentration;nonhuman;nuclear magnetic resonance;plant leaf;polymerase chain reaction;Staphylococcus aureus;synergistic effect;biosynthesis;chemistry;combination drug therapy;down regulation;drug effect;drug potentiation;Eleutherococcus;metabolism;methicillin resistance;methicillin resistant Staphylococcus aureus;microbial sensitivity test;Acanthopanax;Bacteria (microorganisms);methicillin resistant Staphylococcus aureus;Ranellidae;Staphylococcus aureus;Anti-Bacterial Agents;Dicyclohexylcarbodiimide;Down-Regulation;Drug Synergism;Drug Therapy, Combination;Eleutherococcus;Methicillin Resistance;Methicillin-Resistant Staphylococcus aureus;Microbial Sensitivity Tests;Octoxynol;Oxacillin;Penicillin-Binding Proteins;Plant Extracts;Plant Leaves

摘要： Acanthopanax (A.) henryi (Oliv.) Harms contain many bioactive compounds commonly used in traditional Chinese medicine. The objective of the present study was to investigate the antibacterial activity of the single constituent, Eleutheroside K (ETSK) isolated from the leaves of A. henryi (Oliv.) Harms, against methicillin-resistant Staphylococcus (S.) aureus (MRSA). Broth microdilution assay was used to measure the minimal inhibitory concentration (MIC) and the MIC values of ETSK against eight clinical S. aureus strains were all 50µgml−1. At sub-inhibitory concentrations, a synergistic effect between oxacillin (OXA) and ETSK was confirmed using checkerboard dilution assay and time-kill curve analysis. The bacteriostatic effect became more pronounced when ETSK was used in combination with detergent (Triton X-100) or ATPase inhibitor (N, N′-dicyclohexylcarbodiimide). According to western blot analysis, the down-regulated expression of Penicillin-binding protein 2a (PBP2a) further validated that the bacterial activity was inhibited when treated with ETSK in a dose-dependent manner. Results based on our study verified that ETSK significantly suppressed MRSA infections and emphasized the potential application of ETSK as a novel anti-MRSA natural drug. © 2020 The Society for Applied Microbiology

语种： 英文

作者： Deepa, Ponnuvel;Bae, Ho Jung;Park, Hyeon-Bae;Kim, So-Yeon;Choi, Ji Woong;...

期刊： Journal of Ethnopharmacology,2020年253:112651 ISSN：0378-8741

通讯作者： Park, SJ;Ryu, JH

作者机构： [Kim, So-Yeon; Deepa, Ponnuvel; Park, SJ; Park, Se Jin; Park, Hyeon-Bae] Kangwon Natl Univ, Sch Nat Resources & Environm Sci, Chunchon, South Korea.;[Ryu, Jong Hoon; Bae, Ho Jung] Kyung Hee Univ, Dept Life & Nanopharmaceut Sci, Seoul, South Korea.;[Ryu, Jong Hoon; Ryu, JH] Kyung Hee Univ, Coll Pharm, Dept Oriental Pharmaceut Sci, Seoul, South Korea.;[Liu, Xiang-Qian] Hunan Univ Chinese Med, Sch Pharm, Changsha, Peoples R China.;[Choi, Ji Woong] Gachon Univ, Coll Pharm, Lab Neuropharmacol, Incheon, South Korea.

通讯机构： [Ryu, JH ; Park, SJ ] K;Kangwon Natl Univ, Sch Nat Resources & Environm Sci, Chunchon, South Korea.;Kyung Hee Univ, Coll Pharm, Dept Oriental Pharmaceut Sci, Seoul, South Korea.

关键词： acetylcholinesterase;cyclic AMP responsive element binding protein;donepezil;Dracocephalum moldavica extract;mitogen activated protein kinase;neuroprotective agent;nootropic agent;oleanolic acid;plant extract;rosmarinic acid;unclassified drug;acetylcholinesterase;cyclic AMP responsive element binding protein;mitogen activated protein kinase;neuroprotective agent;plant extract;scopolamine;animal behavior;animal cell;animal experiment;animal model;animal tissue;Article;brain cortex;controlled study;Dracocephalum moldavica;drug mechanism;enzyme activation;enzyme activity;enzyme phosphorylation;footshock;hippocampal tissue;hippocampus;latent period;locomotion;male;MAPK signaling;memory disorder;Morris water maze test;motor coordination;mouse;neuroprotection;nonhuman;passive avoidance;plant leaf;protein expression;scopolamine-induced cognitive defect;step-through passive avoidance test;animal;avoidance behavior;cognitive defect;drug effect;hippocampus;Institute for Cancer Research mouse;Lamiaceae;maze test;metabolism;phosphorylation;signal transduction;Acetylcholinesterase;Animals;Avoidance Learning;Behavior, Animal;Cognitive Dysfunction;Cyclic AMP Response Element-Binding Protein;Extracellular Signal-Regulated MAP Kinases;Hippocampus;Lamiaceae;Male;Maze Learning;Mice, Inbred ICR;Neuroprotective Agents;Phosphorylation;Plant Extracts;Plant Leaves;Scopolamine;Signal Transduction

摘要： Ethnopharmacological relevance: Dracocephalum moldavica (Moldavian balm) has been traditionally used for the treatment of intellectual disabilities, migraines and cardiovascular problems in East Asia. Recent scientific studies have demonstrated the usefulness of this plant to treat neurodegenerative disorders, including Alzheimer's disease. Aim of the study: This study aimed to investigate the effects of the ethanolic extract of D. moldavica leaves (EEDM) on scopolamine-induced cognitive impairment in mice and the underlying mechanisms of action. Materials and methods: The behavioral effects of EEDM were examined using the step-through passive avoidance and Morris water maze tasks. To elucidate the underlying mechanism, we tested whether EEDM affects acetylcholinesterase activity and the expression of memory-related signaling molecules including extracellular signal-regulated kinase (ERK) and cAMP response element-binding protein (CREB) in the hippocampus. Results: EEDM (25, 50 or 100 mg/kg) significantly ameliorated the scopolamine-induced step-through latency reduction in the passive avoidance task in mice. In the Morris water maze task, EEDM (50 mg/kg) significantly attenuated scopolamine-induced memory impairment. Furthermore, the administration of EEDM increased the phosphorylation levels of ERK and CREB in the hippocampus but did not alter acetylcholinesterase activity. Conclusions: These findings suggest that EEDM significantly attenuates scopolamine-induced memory impairment in mice and may be a promising therapeutic agent for improving memory impairment. © 2020 Elsevier B.V.

语种： 英文

作者： 李小军;金官佑;张晓丹;吴贤哲;金伦喆;...

期刊： 天然产物研究与开发,2020年32(3):427-434 ISSN：1001-6880

作者机构： 赣南医学院药学院国家中药现代化工程技术研究中心客家中医药资源研究分中心,赣州341000;湖南中医药大学药学院,长沙410208;圆光大学药学院,益山54538;浙江理工大学生命科学与医药学院,杭州310000;[李小军] 赣南医学院

关键词： 糙叶五加;黄酮;单萜;木脂素;化学成分;抗炎活性

摘要： 基于脂多糖(LPS)诱导的小胶质细胞BV2为生物活性导向模型首次研究糙叶五加Acanthopanax henryi(Oliv.) Harms果实的化学成分.采用正反相硅胶柱色谱、凝胶柱色谱、制备薄层、制备液相及重结晶等方法进行分离纯化,利用波谱分析结合理化性质鉴定化合物的结构.从糙叶五加果实甲醇提取物的乙酸乙酯萃取部位中分离得到18个化合物,分别鉴定为5-羟甲基-2-糠醛(1)、5-羟基麦芽酚(2)、原儿茶酸(3)、6-甲氧基-7-羟基香豆素(4)、山柰酚-3-O-β-D-葡萄糖苷(5)、山奈酚-3-芸香苷(6)、(-)-松脂醇4-O-β-D-吡喃葡萄糖苷(7)、(+)-simplexoside (8)、(-)-芝麻素(9)、松香(10)、苯甲基-β-D-吡喃葡萄糖苷-6'-O-乙酸酯(11)、3,4-二羟基-p-薄荷-1-烯(12)、(4R)-p-薄荷-1-烯-4,7-二醇(13)、(2E,6R)-2,6-二甲基-2,7-辛二烯-1,6-二.醇(14)、(+)-(3S,4S,6R)-3,6-二羟基-1-薄荷烯(15)、齐墩果酸-3-O-β-D-葡萄糖醛酸苷(16)、styraxlignolide E(17)、styraxlignolide D (18).化合物2、8、10～15、17、18为首次从五加科植物中分离得到;化合物1和7为首次从五加属植物中分离得到;除化合物4～6、9、16外,其他化合物均为首次从该植物中分离得到.抗炎活性筛选结果表明,被测试化合物均表现出了一定的NO抑制活性,其中,化合物1、4、7、9、12、13、18表现出了适度的抑制NO生成的活性.

语种： 中文

作者： 肖珊;罗姣;许忠;黄完均;肖瑾;...

期刊： 中草药,2020年51(14):3791-3796 ISSN：0253-2670

通讯作者： Liu, X.-Q.

作者机构： [肖瑾; 鲁曼霞; 肖珊; 罗姣; 刘向前; 卢茂芳] School of Pharmacy, Hunan University of Chinese Medicine, Changsha, 410208, China;[许忠] Economic Crop Technology Promotion Station, Yunnan Mojiang Hani Autonomous County, Pu'er, 654800, China;[黄完均] College of Pharmacy, Chung-Ang University, Seoul, 156756, South Korea

通讯机构： [Liu, X.-Q.] S;School of Pharmacy, China

关键词： 五加属;苯丙素类;紫丁香苷;绿原酸;咖啡酸;对香豆酸;阿魏酸;(+)-梣皮树脂醇;(±)-松脂素;(±)-丁香脂素

摘要： 目的建立以HPLC法同时检测16种33批五加属植物中紫丁香苷、绿原酸、咖啡酸、对香豆酸、阿魏酸、ethylsyringin、 ethylconiferin、(+)-梣皮树脂醇、(±)-松脂素、(±)-丁香脂素、taiwanin C、savinin、helioxanthin、(–)-sesamin 14种苯丙素类成分的含量。方法采用Promosil C_(18)柱(250 mm×4.6 mm,5 μm);流动相为乙腈-0.1%磷酸水溶液,梯度洗脱,体积流量1 mL/min;柱温30 ℃;检测波长280 nm。结果在优化的色谱条件下,14种成分在测定的质量浓度范围内线性关系良好,r均大于0.999 1,方法的精密度、稳定性、重复性以及回收率良好。结论建立的HPLC法可用于同时检测五加属植物中14种苯丙素类化学成分的含量,该方法快速、准确,有较好的重现性和稳定性,可为该属植物的进一步研究提供参考。

语种： 中文

作者： 肖瑾;肖珊;许忠;罗姣;鲁曼霞;...

期刊： 中药材,2020年(09):2181-2188 ISSN：1001-4454

作者机构： 湖南中医药大学药学院;云南省墨江哈尼族自治县经济作物技术推广站

关键词： 五加属植物;黄酮;含量测定

摘要： 目的：建立HPLC法同时测定16种33批五加属植物中apigenin-6,8-C-di-β-D-glucopyranoside、芦丁、金丝桃苷、柚皮苷、芹菜素-7-O-新橙皮苷、大波斯菊苷、槲皮素、芹菜素、山柰素、异鼠李素10种黄酮类成分的含量。方法：采用Promosil C18（250 mm×4.6 mm,5μm）色谱柱；流动相为乙腈-0.1%磷酸溶液，梯度洗脱；流速为1.0 mL/min；柱温为30℃；检测波长为283 nm。结果：10种成分在相应的浓度范围内与峰面积的线性关系良好，R2>0.9991，精密度、稳定性、重复性以及加样回收率试验RSD<3.00%。结论：该研究建立的HPLC法可用于同时检测五加属植物中的10种黄酮类成分的含量，方法高效、准确，重现性和稳定性良好，可为该属植物的进一步研究提供参考。

语种： 中文

作者： Yang, Yang;Luo, Jiao;Lu, Man-Xia;Zhang, Hong-Chi;An, Zhi-Peng;...

期刊： Acta Medica Mediterranea,2020年36(1):517-522 ISSN：0393-6384

通讯作者： Liu, XQ;Hwang, EHE

作者机构： [Zhang, Hong-Chi; An, Zhi-Peng; Yang, Yang] Shanxi Datong Univ, Sch Life Sci, Datong, Peoples R China.;[Liu, XQ; Lu, Man-Xia; Liu, Xiang-Qian; Luo, Jiao] Hunan Univ Chinese Med, Sch Pharm, Changsha 410208, Hunan, Peoples R China.;[Hwang, Eun-Hee; Hwang, EHE] Wonkwang Univ, Dept Food & Nutr, Iksan 54538, South Korea.

通讯机构： [Liu, XQ ] H;[Hwang, EHE ] W;Hunan Univ Chinese Med, Sch Pharm, Changsha 410208, Hunan, Peoples R China.;Wonkwang Univ, Dept Food & Nutr, Iksan 54538, South Korea.

关键词： Diabetes mellitus;Diabetic neuropathy;Kidney;Streptozotocin

摘要： Acanthopanax gracilistylus is traditionally used to treat bone pains, arthritis, and rheumatism by the rook bark. To evaluate the renoprotective effect of the acankoreagenin, from the leaves of Acanthopanax gracilistylus (LAG), glucose loaded and streptozotocin (STZ) induced diabetic rats. The diabetic nephropathy (DN) rats were induced with STZ (70 mg/kg) by a single intraperitoneal injection. The acankoreagenin, was administered orally to normal and diabetic rats with guava extract and solvent control as comparison groups. Long term effects like kidney function, tumor necrosis factor-alpha (TNF-a) level, oxidative stress biomarkers, histopathology of kidney tissues were investigated. The results showed that acankoreagenin (2 mg/kg) treatment showed significant reduction in kidney function and TNF-a levels. The kidney tissue was used for immunohistochemical and histopathological measurement with transforming growth factor beta (TGF-β). Treatments with 2 mg/kg acankoreagenin group resulted renal oxidative stress (glutathione lever was increased from 0.36 to 1.53 uM/mg protein). Through inhibiting the TGF-β, the renal fibrosis was suppressed in STZ-induced diabetic rats. In renal tissues, histopathological injury was also observed. These findings are in support of the beneficial effect with the acankoreagenin treatment in antidiabetic and DN effects in streptozotocin induced diabetic rats. © 2020 A. CARBONE Editore. All rights reserved.

语种： 英文

作者： 肖瑾;李小军;黄建军;高圣权;陆昌洙;...

期刊： 中草药,2020年51(22):5781-5787 ISSN：0253-2670

通讯作者： Liu, X.-Q.

作者机构： [刘向前; 肖瑾] School of Pharmacy, Hunan University of Chinese Medicine, Changsha, 410208, China;[李小军] College of Pharmaceutical Sciences, Gannan Medical University, Ganzhou, 341000, China;[黄建军] Department of Clinical Biochemistry, Xiangya Medical College, Central South University, Changsha, 410013, China;[Ko S.-K.] Department of Oriental Medical Food & Nutrition, Semyung University, Jecheon, 27136, South Korea;[Yook C.-S.] College of Pharmacy, Kyung Hee University, Seoul, 130701, South Korea

通讯机构： [Liu, X.-Q.] S;School of Pharmacy, China

关键词： 细柱五加;三萜;抗炎;高迁移率族蛋白B1;核因子-κB

摘要： 目的 研究细柱五加Acanthopanax gracilistylus W.W.Smith中impressic acid(IA)对脂多糖(LPS)诱导的小鼠单核巨噬细胞RAW264.7的抗炎作用.方法 以LPS诱导RAW264.7细胞建立炎症模型;使用EZ4U细胞增殖与细胞毒性分析试剂盒检测IA对RAW264.7细胞的毒性作用;Griess法测定一氧化氮(NO)水平;ELISA法测肿瘤坏死因子-a(TNF-a)、白介素-1β(IL-1β)水平;RT-PCR法检测TNF-α、IL-1β mRNA表达;Western blotting检测高迁移率族蛋白B1(HMGB1)蛋白表达;ELISA法细胞质和细胞核的核因子-κB(NF-κB)水平.结果 Impressic acid可以显著抑制LPS诱导的RAW264.7细胞中TNF-a、IL-1β水平和HMGB1蛋白表达,并抑制NF-κB从细胞质向细胞核的转移(P＜0.05、0.01).结论 细柱五加中IA对LPS诱导的RAW264.7细胞具有抗炎作用.

语种： 中文

作者： 张斌贝;李小军;高大林;肖珊;鲁曼霞;...

期刊： 湖南中医药大学学报,2019年39(04):556-561 ISSN：1674-070X

作者机构： 湖南中医药大学药学院,湖南 长沙,410208;湖南中医药大学药学院,湖南 长沙 410208;圆光大学药学院,韩国 益山 54538;[李小军; 高大林; 卢茂芳; 刘向前; 张斌贝; 鲁曼霞; 肖珊] 湖南中医药大学

关键词： 五加属;化学成分;多样性;萜类

摘要： 五加属植物隶属五加科,其广泛的地理分布、不同的生长环境以及在植物进化中所扮演的重要角色使其产生了大量结构新颖的二次代谢产物,并呈现出一定的化学多样性。目前为止,国内外学者已经从五加属植物发现并报道的化学成分主要有挥发油、萜类、木脂素类、黄酮类、酚酸类等多种结构类型。本文对近十年来五加属植物化学成分的国内外研究概况进行综述,以期为五加属植物深入研究与开发提供有益参考。

语种： 中文

作者： 李小军;金官佑;吴贤哲;金伦喆;刘向前

期刊： 中草药,2019年50(5):1055-1060 ISSN：0253-2670

通讯作者： Liu, X.-Q.

作者机构： 湖南中医药大学药学院, 湖南, 长沙, 410208;圆光大学药学院, 韩国, 益山, 54538;[金官佑] 金官佑;[李小军] 李小军;[刘向前] 刘向前

通讯机构： [Liu, X.-Q.] S;School of Pharmacy, China

关键词： 糙叶五加;反式松柏醛;丁香醛;反式芥子醛;咖啡酰奎宁酸;十一烷二酸单甲酯

摘要： 目的 基于脂多糖(LPS)诱导的巨噬细胞RAW264.7和小胶质细胞BV2为生物活性导向模型研究糙叶五加Acanthopanax henryi茎的化学成分.方法 采用硅胶柱色谱、凝胶柱色谱、制备薄层及重结晶等方法进行分离纯化,利用波谱分析结合理化性质鉴定化合物的结构.结果 从糙叶五加茎甲醇提取物的醋酸乙酯萃取部位中分离得到18个化合物,分别鉴定为对羟基苯甲酸(1)、反式对羟基肉桂酸(2)、反式咖啡酸甲酯(3)、咖啡酸(4)、反式松柏醛(5)、丁香醛(6)、香草醛(7)、6-甲氧基-7-羟基香豆素(8)、反式芥子醛(9)、十一烷二酸单甲酯(10)、(-)-芝麻素(11)、3-O-咖啡酰奎宁酸(12)、5-O-咖啡酰奎宁酸(13)、1,3-双咖啡酰奎宁酸(14)、1,4-双咖啡酰奎宁酸(15)、1,5-双咖啡酰奎宁酸(16)、豆甾醇(17)、β-谷甾醇(18).结论 化合物10为首次从五加科植物中分离得到;除化合物12、14、17、18外,其他化合物均为首次从该植物中分离得到.

语种： 中文

作者： Li, Xiao-Jun;Kim, Kwan-Woo;Kim, Dong-Cheol;Oh, Hyuncheol;Liu, Xiang-Qian;...

期刊： Natural Product Research,2019年35(8):1299-1306 ISSN：1478-6419

通讯作者： Liu, XQ;Kim, YC

作者机构： [Li, Xiao-Jun] Gannan Med Univ, Coll Pharmaceut Sci, Ganzhou 341000, Jiangxi, Peoples R China.;[Oh, Hyuncheol; Kim, Kwan-Woo; Li, Xiao-Jun; Kim, Dong-Cheol; Kim, Youn-Chul] Wonkwang Univ, Coll Pharm, Inst Pharmaceut Res & Dev, Lksan 54538, South Korea.;[Liu, XQ; Liu, Xiang-Qian] Hunan Univ Chinese Med, Sch Pharm, Changsha 410208, Hunan, Peoples R China.;[Oh, Hyuncheol; Kim, Kwan-Woo; Kim, Dong-Cheol; Kim, Youn-Chul] Wonkwang Univ, Hanbang Cardio Renal Syndrome Res Ctr, Iksan 54538, South Korea.;[Kim, Youn-Chul] Wonkwang Univ, Coll Pharm, Iksan 54538, South Korea.

通讯机构： [Liu, XQ ] H;[Kim, YC ] W;Hunan Univ Chinese Med, Sch Pharm, Changsha 410208, Hunan, Peoples R China.;Wonkwang Univ, Coll Pharm, Iksan 54538, South Korea.

关键词： 4,7 dihydroxy 1 menthene 7 o beta glucopyranoside;6 hydroxy 2,6 dimethyl 2,7 octadien 1 yl (6' o acetyl) o beta glucopyranoside;antiinflammatory agent;eleuhenryiside A;eleuhenryiside B;eleuhenryiside C;Eleutherococcus henryi extract;kobusin;lipopolysaccharide;monoterpenoid;nitric oxide;plant glycoside;unclassified drug;1,3 benzodioxole derivative;glycoside;kobusin;lignan;lipopolysaccharide;nonsteroid antiinflammatory agent;terpene;antiinflammatory activity;Article;BV-2 cell line;controlled study;drug isolation;drug screening;drug structure;Eleutherococcus;Eleutherococcus henryi;fruit;IC50;nervous system inflammation;nuclear magnetic resonance spectroscopy;RAW 264.7 cell line;animal;chemical structure;chemistry;drug effect;fruit;microglia;mouse;preclinical study;Animals;Anti-Inflammatory Agents, Non-Steroidal;Benzodioxoles;Drug Evaluation, Preclinical;Eleutherococcus;Fruit;Glycosides;Lignans;Lipopolysaccharides;Magnetic Resonance Spectroscopy;Mice;Microglia;Molecular Structure;Monoterpenes;RAW 264.7 Cells

摘要： Abstract: The phytochemical investigation on the fruits of Eleutherococcus henryi (Araliaceae) resulted in the discovery of three novel monoterpene glycosides, eleuhenryiside A (1), eleuhenryiside B (2), and eleuhenryiside C (3), as well as a known lignan, (-)-kobusin (4). Their chemical structures were elucidated by mass, 1 D- and 2 D-NMR spectroscopy. The chemical structures of new compounds 1-3 were determined to be (2E,6R)-6-hydroxy-2,6-dimethyl-2,7-octadien-1-yl-(6’-O-acetyl)-O-β-glucopyranoside, (2Z,6R)-6-hydroxy-2,6-dimethyl-2,7-octadien-1-yl-(6’-O-acetyl)-O-β-glucopyranoside, and (-)-(4 R)-4,7-dihydroxy-1-menthene 7-O-β-glucopyranoside, respectively. The anti-neuroinflammatory and anti-inflammatory activities of these compounds were evaluated with LPS-stimulated BV2 microglia and RAW264.7 macrophage, respectively. The results showed that new compounds 1 and 3 have inhibitory effects of NO production with IC50 values of 32.50 ± 1.60 and 3.54 ± 0.20 μM in LPS-stimulated BV2 microglia. Also, (-)-kobusin (4) has abilities to inhibit NO production with the IC50 values of 14.25 ± 2.69 and 36.35 ± 6.27 μM in BV2 and RAW264.7 cells, respectively, which indicated that it may possess the potential anti-neuroinflammatory and anti-inflammatory activities. © 2019 Informa UK Limited, trading as Taylor & Francis Group.

语种： 英文

作者： Li, Xiao-Jun;Kim, Kwan-Woo;Zou, Qin-Peng;Liu, Zu-Zhen;Oh, Hyun-Cheol;...

期刊： Natural Product Communications,2019年14(1):103-106 ISSN：1934-578X

通讯作者： Liu, XQ;Kim, YC

作者机构： [Liu, XQ; Li, Xiao-Jun; Liu, Xiang-Qian] Hunan Univ Chinese Med, Sch Pharm, Changsha 410208, Hunan, Peoples R China.;[Kim, Youn-Chul; Kim, Kwan-Woo; Li, Xiao-Jun; Oh, Hyun-Cheol] Wonkwang Univ, Coll Pharm, Iksan 570749, South Korea.;[Liu, Zu-Zhen] Hunan Soc Chinese Med, Changsha 410008, Hunan, Peoples R China.;[Zou, Qin-Peng] Broad Ocean Biosci & Tech Co Ltd Changsha, Changsha 410205, Hunan, Peoples R China.

通讯机构： [Liu, XQ ] H;[Kim, YC ] W;Hunan Univ Chinese Med, Sch Pharm, Changsha 410208, Hunan, Peoples R China.;Wonkwang Univ, Coll Pharm, Iksan 570749, South Korea.

关键词： 24 methylene 5alpha dammara 20(21) en 3 beta yl acetate;antiinflammatory agent;carbene;coumarin;glycoside;Pileostegia viburnoides extract;plant extract;steroid;triterpene derivative;triterpenoid;unclassified drug;animal cell;Article;chemical analysis;controlled study;drug structure;medicinal plant;melting point;nonhuman;optical rotation;physical chemistry;Pileostegia viburnoides;plant stem;proton nuclear magnetic resonance

摘要： Phytochemical investigation on the stems of Pileostegia viburnoides Hook.f.et Thoms led to the isolation of twelve compounds, including two steroids (1 and 10), two fatty compounds (2 and 12), seven triterpenoids (3-9), and one coumarin glycoside (11). 24-methylene-5α-dammara-20(21)-en-3β-yl acetate (5), a rare dammarane-tetracyclic triterpene had been first reported just according to the typical 1H-NMR signals analysis, melting point and optical rotation in 1975. However, the complete and reliable NMR and mass data were still unknown. This is the first time to identify the planar and spatial structures of compound 5 by the analysis of 1D-, 2D-NMR, mass data and other physicochemical properties, as well as by comparison of the spectral data with the literature. Further more, the anti-inflammatory and anti-neuroinflammatory activities of dammarane-type triterpenes 4 and 5 were evaluated. © 2019 Natural Product Communications. All rights reserved.

语种： 英文

作者： Han, Yo-Han;Kee, Ji-Ye;Park, Seong-Hwan;Mun, Jeong-Geon;Jeon, Hee-Dong;...

期刊： Phytomedicine,2019年62:152952 ISSN：0944-7113

通讯作者： Hong, SH

作者机构： [Kee, Ji-Ye; Jeon, Hee-Dong; Hong, Seung-Heon; Mun, Jeong-Geon; Hong, SH; Han, Yo-Han; Park, Seong-Hwan] Wonkwang Univ, Wonkwang Oriental Med Res Inst, Coll Pharm, Dept Oriental Pharm, 460 Iksandae Ro, Iksan 54538, Jeonbuk, South Korea.;[Park, Seong-Hwan] Korea Inst Oriental Med, Clin Med Div, Daejeon, South Korea.;[Park, Jinbong] Kyung Hee Univ, Coll Korean Med, Dept Pharmacol, 26 Kyungheedae Ro, Seoul 02447, South Korea.;[Zou, Qin-Peng] Changsha Broad Ocean Biosci & Tech Co Ltd, Changsha 410205, Hunan, Peoples R China.;[Liu, Xiang-Qian] Hunan Univ Chinese Med, Sch Pharm, Changsha 410208, Hunan, Peoples R China.

通讯机构： [Hong, SH ] W;Wonkwang Univ, Wonkwang Oriental Med Res Inst, Coll Pharm, Dept Oriental Pharm, 460 Iksandae Ro, Iksan 54538, Jeonbuk, South Korea.

关键词： 3T3-L1;AMPK;Cassiae tora seed;Human adipose mesenchymal stem cells;Rubrofusarin-6-beta-gentiobioside;mTOR

摘要： BACKGROUND: Although rubrofusarin-6-beta-gentiobioside (RFG), which is a component of Cassiae tora seed, could likely regulate hyperlipidemia, its anti-obesity effect and related mechanism have not been elucidated. PURPOSE: The aim of this study was to examine whether RFG can ameliorate obesity and the mechanism of lipid accumulation regulated by RFG. STUDY DESIGN: In in vitro experiments, we confirmed the anti-adipogenic effect of RFG using 3T3-L1 cells and human adipose mesenchymal stem cells (hAMSCs). To confirm the anti-obesity effect, High-Fat Diet (HFD)-induced obese mice were selected as a model. METHODS: We investigated anti-adipogenic effects of RFG using MTS assay, Oil Red O Staining, real-time RT-PCR, western blot analysis, and immunofluorescence staining. The anti-obesity effect of RFG was confirmed in HFD-induced mice model using hematoxylin and eosin staining and serum analysis. RESULTS: RFG inhibited lipid accumulation in 3T3-L1 cells and hAMSCs by reducing expression of mammalian targets of rapamycin (mTOR), peroxisome proliferator-activated receptor (PPAR)gamma, and CCAAT-enhancer binding protein (C/EBP)alpha. RFG phosphorylated AMP-activated protein kinase (AMPK) in a liver kinase B (LKB) 1-independent manner. Moreover, the anti-adipogenic effect of RFG was blocked by AMPK inhibitor. These results suggest that RFG inhibits lipid accumulation via AMPK signaling. Furthermore, RFG reduced the body weight, size of epididymal white adipose tissue (eWAT), and fatty liver in the mice. RFG also suppressed levels of adipogenic factors PPARgamma, C/EBPalpha, FAS, LPL, and aP2) by activating AMPK in the eWAT and liver. CONCLUSION: RFG can ameliorate obesity, and thus, could be used as a therapeutic agent for treating obesity.

语种： 英文

作者： 李小军;吴贤哲;金伦喆;刘向前

期刊： 中成药,2019年41(8):1856-1862 ISSN：1001-1528

作者机构： 湖南中医药大学药学院,湖南长沙410208;圆光大学药学院,韩国益山54538;赣南医学院药学院,江西赣州341000;湖南中医药大学药学院,湖南长沙,410208;[李小军; 刘向前] 湖南中医药大学

关键词： 糙叶五加;花;化学成分;分离鉴定;抗炎活性

摘要： 目的研究糙叶五加Acanthopanax henryi( Oliv.) Harms花的化学成分及其粗提物抗炎活性。方法糙叶五加花70%甲醇提取物乙酸乙酯部位采用正相硅胶、反相C_(18)柱、Sephadex LH-20、HPLC、制备薄层和重结晶等进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构。采用脂多糖( LPS)诱导的巨噬细胞RAW264.7和小胶质细胞BV2为筛选模型评价提取物抗炎活性。结果从中分离得到17个化合物,分别鉴定为槲皮素-3-O-β-D-吡喃葡萄糖苷( 1) 、山柰酚-3-O-β-D-吡喃葡萄糖苷( 2) 、山柰酚-3-O-α-L-鼠李糖苷( 3) 、山柰酚-3-芸香糖苷( 4) 、1,5-双咖啡酰奎宁酸( 5) 、1,4-双咖啡酰奎宁酸( 6) 、1,3-双咖啡酰奎宁酸( 7) 、4,5-双咖啡酰奎宁酸( 8) 、3,4-双咖啡酰奎宁酸( 9) 、3,5-双咖啡酰奎宁酸( 10) 、山柰酚( 11) 、芦丁( 12) 、二氢芝麻素-9-O-β-D-吡喃葡萄糖苷( 13) 、丁香酚苷( 14) 、3,5-双咖啡酰奎宁酸甲酯( 15) 、3,4-双咖啡酰奎宁酸甲酯( 16) 、1,3-双咖啡酰奎宁酸甲酯( 17) 。乙酸乙酯部位具有较强的抗炎活性。结论所有化合物均为首次从该植物中分离得到。

语种： 中文

作者： 高大林;罗姣;张斌贝;卢茂芳;鲁曼霞;...

期刊： 中国药师,2019年22(9):1725-1728 ISSN：1008-049X

作者机构： 湖南中医药大学药学院 长沙410208;[高大林; 卢茂芳; 刘向前; 张斌贝; 鲁曼霞; 罗姣] 湖南中医药大学

关键词： 吴茱萸五加;高效液相色谱;黄酮;含量测定

摘要： 目的:采用HPLC法同时检测吴茱萸五加叶中8种黄酮类成分的含量,以期为该植物的进一步研究提供参考.方法:采用Promosil C18柱(250 mm×4.6 mm,5μm),流动相为乙腈-0.1％磷酸,梯度洗脱,流速1.0 ml·min-1,柱温30℃,检测波长283 nm.结果:在优化的色谱条件下,8种成分均有良好的分离度,以及良好的线性关系,方法精密度RSD＜2.0％,待测样品于室温下24h内能保持稳定,apig-enin-6,8-C-di-β-D-glucopyranoside、芦丁、金丝桃苷、柚皮苷、樱桃苷、芹菜素-7-O-新橙皮苷、大波斯菊苷、槲皮素的线性范围分别为7.32～ 146.40 μg·ml-1(r1 =0.999 7)、10.20～204.00 μg·ml-1(r2=0.999 2)、4.26～85.20 μg·ml-1(r3 =0.999 2)、11.69～233.92 μg·ml-1(r4 =0.999 1)、2.32～46.40 μg·ml-1(r5 =0.999 2)、1.25～24.96μg·ml-1(r6 =0.999 4)、2.80～56.00 μg·ml-1(r7 =0.999 5)、3.39～67.84 μg·ml-1(r8=0.999 3),平均加样回收率分别为100.02％(RSD=0.96％)、99.87％(RSD=1.2％)、101.03％(RSD=2.1％)、98.23％(RSD=2.3％)、99.01％(RSD=1.6％)、99.22％(RD=2.7％)、99.02％(RSD=2.0％)、99.11％(RSD=2.5％)(n=6).结论:该方法操作简单、快捷,有很好的重现性和稳定性,可为该植物的进一步研究提供参考.

语种： 中文

作者： 肖珊;罗姣;张斌贝;高大林;刘向前

期刊： 中草药,2019年50(4):868-874 ISSN：0253-2670

通讯作者： Liu, X.-Q.

作者机构： 湖南中医药大学药学院, 湖南, 长沙, 410208;[张斌贝] 张斌贝;[罗姣] 罗姣;[肖珊] 肖珊;[高大林] 高大林

通讯机构： [Liu, X.-Q.] S;School of Pharmacy, China

关键词： 响应面法;聚酰胺树脂;吴茱萸五加;柚皮苷;纯化;单因素实验

摘要： 目的利用响应面设计法优化聚酰胺树脂纯化吴茱萸五加Acanthopanax evodiaefolius叶中柚皮苷的工艺。方法对上样质量浓度、上柱样用量、洗脱体系、洗脱剂用量、体积流量5个因素进行单因素考察,并使用响应曲面设计法优化筛选出聚酰胺树脂纯化吴茱萸五加叶中柚皮苷的最优工艺条件。结果吴茱萸五加叶中的柚皮苷的最优纯化工艺条件为上柱样质量浓度为4.0 mg/mL,上样量体积为3.5 BV,洗脱体系为30%甲醇,洗脱剂用量为3.0 BV,洗脱体积流量为8.0 BV/h。在该条件下,柚皮苷质量分数由5.08%提高到56.12%,得率为41.69%。再经一步重结晶后质量分数达到90%以上,符合医药原料要求。结论聚酰胺树脂对吴茱萸五加叶中柚皮苷的纯化效果好,操作简单,效率高,稳定性好,可用于工业推广。

语种： 中文

作者： 岑叶盛;李小龙;陈淑娟;刘向前;曾庆钱;...

期刊： 中草药,2019年50(13):3071-3076 ISSN：0253-2670

通讯作者： Wang, H.-L.

作者机构： 浙江理工大学生命科学与医药学院, 浙江, 杭州, 310018;湖南职业技术学院药学院, 湖南, 长沙, 410000;湖南中医药大学药学院, 湖南, 长沙, 410208;广东省中药研究所, 广东, 广州, 510520;[岑叶盛] 岑叶盛

通讯机构： [Wang, H.-L.] C;College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, China

关键词： 白簕叶;总多酚;大孔吸附树脂;纯化工艺;Folin-Ciocalteu比色法;吸附;解吸

摘要： 目的 考察大孔吸附树脂对白筋叶总多酚的吸附性能和纯化效果,寻找白筋叶总多酚纯化的较优工艺.方法 采用Folin-Ciocalteu比色法定量,比较5种不同型号大孔吸附树脂对药食同源植物白簕叶中总多酚的吸附和解吸效果,从中筛选出适合分离纯化白簕叶总多酚的树脂,并对其吸附和解吸条件进行考察和优化.经过单因素实验,确定最终优化工艺.结果 所得优化工艺为以质量浓度为1.0 mg/mL,pH值为3.0左右的白簕叶总多酚粗提液作为上样液,同时控制其体积流量为2.0mL/min,上样量控制为30 mL;然后以体积分数50％的乙醇水溶液洗脱,调节pH值为6.0,并控制洗脱体积流量为2.0 mL/min,洗脱量为40 mL.经HPD100型树脂纯化后,白筋叶多酚样品质量分数由纯化前的11.7％上升为49.7％,纯化效果为4.25倍.通过将HPD100型树脂进行扩大30倍实验证实:白筋叶多酚样品质量分数由纯化前的12.5％上升为54.5％,纯化效果为4.4倍,纯化效率并不随大孔树脂数量改变而改变.结论 HPD100为纯化白筋叶总多酚的最佳树脂,该工艺适合白筋叶总多酚的提取纯化工业生产.

语种： 中文

作者： Zou, Qin-Peng;Liu, Xiang-Qian;Huang, Jian-Jun;Yook, Chang-Soo;Whang, Wan-Kyunn;...

期刊： MOLECULAR MEDICINE REPORTS,2017年16(6):9149-9156 ISSN：1791-2997

通讯作者： Liu, XQ

作者机构： [Zou, Qin-Peng] Changsha Broad Ocean Biosci & Tech Co Ltd, Changsha 410205, Hunan, Peoples R China.;[Liu, Xiang-Qian] Hunan Univ Chinese Med, Sch Pharm, Changsha 410208, Hunan, Peoples R China.;[Huang, Jian-Jun] Cent S Univ, Dept Clin Biochem, Xiangya Med Coll, Changsha 410013, Hunan, Peoples R China.;[Yook, Chang-Soo] Kyung Hee Univ, Coll Pharm, Seoul 130701, South Korea.;[Whang, Wan-Kyunn] Chung Ang Univ, Coll Pharm, Seoul 156756, South Korea.

通讯机构： [Liu, XQ ] H;Hunan Univ Chinese Med, Sch Pharm, Hanpu Sci & Educ, 300 Xueshi Rd, Changsha 410208, Hunan, Peoples R China.

关键词： 1,3 o beta dextro glucopyranosyl 3alpha,11alpha dihydroxylup 20(29) en 28 oic acid;acankoreoside A;acankoreoside B;acankoreoside D;Acanthopanax gracilistylus extract;acantrifoside A;dexamethasone;high mobility group B1 protein;interleukin 1beta;lipopolysaccharide;messenger RNA;saponin;triterpenoid;tumor necrosis factor;unclassified drug;high mobility group B1 protein;immunoglobulin enhancer binding protein;interleukin 1beta;lupane;saponin;triterpene;tumor necrosis factor;Acanthopanax;Acanthopanax gracilistylus;animal cell;antiinflammatory activity;Article;cell viability;controlled study;cytokine release;down regulation;drug effect;drug mechanism;enzyme linked immunosorbent assay;gene expression;macrophage;mouse;nonhuman;plant leaf;protein expression;RAW 264.7 cell line;reverse transcription polymerase chain reaction;Western blotting;animal;biosynthesis;cell survival;gene expression regulation;genetics;immunology;macrophage;metabolism;physiology;Animals;Cell Survival;Gene Expression Regulation;HMGB1 Protein;Interleukin-1beta;Lipopolysaccharides;Macrophages;Mice;NF-kappa B;RAW 264.7 Cells;Saponins;Triterpenes;Tumor Necrosis Factor-alpha

摘要： Acanthopanax gracilistylus (AGS) has long been used in traditional Chinese medicine for the treatment of various inflammatory diseases. 3-O-β-D-glucopyranosyl 3α, 11α-dihydroxylup-20(29)-en-28-oic acid, acantrifoside A, acankoreoside D, acankoreoside B and acankoreoside A are major lupane-type triterpenoid saponins derived from AGS. In the present study, these five saponins were isolated from AGS by chromatography and their anti-inflammatory activities were investigated in lipopolysaccharide (LPS)-treated RAW264.7 macrophages. Cell viability was evaluated by MTT assay. Tumor necrosis factor (TNF)-α, interleukin (IL)-1β and NF-κB p65 were measured by ELISA. The gene expression levels of TNF-α and IL-1β was detected by reverse-transcription polymerase chain reaction. And high-mobility group box 1 (HMGB1) were analyzed by western blotting. The results demonstrated that these five saponins significantly suppressed LPS-induced expression of TNF-α and IL-1β at the mRNA and protein level in RAW264.7 cells. Further analysis revealed that acankoreoside A and acankoreoside B were able to reduce the secretion of HMGB1 and NF-κB activity induced by LPS in RAW264.7 macrophages. Taken together, these results suggested that the anti-inflammatory activity of AGS-derived saponins may be associated with the downregulation of TNF-α and IL-1β, and the 'late-phase' proinflammatory cytokine HMGB1, via negative regulation of the NF-κB pathway in RAW264.7 cells.

语种： 英文

作者： 刘向前;李小军;金伦喆;陆昌洙

期刊： 湖南中医药大学学报,2017年37(1):87-105 ISSN：1674-070X

作者机构： 湖南中医药大学药学院,湖南 长沙,410208;湖南中医药大学药学院，湖南 长沙 410208;圆光大学药学院，韩国 益山 570749;圆光大学药学院,韩国 益山,570749;庆熙大学药学院,韩国 首尔,130701

关键词： 木栓烷型三萜;核磁波谱特征

摘要： 对天然产物中发现的木栓烷型三萜化合物的13C-NMR、1H-NMR谱学特征进行综述,以期减少天然产物特别是木栓烷型三萜结构鉴定工作的盲目性和重复性,为进一步研究分析木栓烷型三萜提供经验借鉴。

语种： 中文