作者：
Wang, S.;Luo, J.;Liu, X. -Q.;Kang, O. -H.*;Kwon, D. -Y.*
期刊：
Letters in Applied Microbiology,2021年72(3):238-244 ISSN：0266-8254
通讯作者：
Kang, O. -H.;Kwon, D. -Y.
作者机构：
[Kang, O. -H.; Kwon, DY; Wang, S.; Kwon, D. -Y.] Wonkwang Univ, Dept Oriental Pharm, Coll Pharm, Iksan 54538, Jeonbuk, South Korea.;[Kang, O. -H.; Kwon, DY; Wang, S.; Kwon, D. -Y.] Wonkwang Univ, Wonkwang Oriental Med Res Inst, Iksan 54538, Jeonbuk, South Korea.;[Liu, X. -Q.; Luo, J.] Hunan Univ Chinese Med, Sch Pharm, Changsha, Hunan, Peoples R China.
通讯机构：
[Kang, OH; Kwon, DY] W;Wonkwang Univ, Dept Oriental Pharm, Coll Pharm, Iksan 54538, Jeonbuk, South Korea.;Wonkwang Univ, Wonkwang Oriental Med Res Inst, Iksan 54538, Jeonbuk, South Korea.
关键词：
mecA;methicillin-resistant Staphylococcus aureus;penicillin-binding protein 2a;sanguisorbigenin;synergy;β-lactam antibiotics
摘要：
The present study evaluated the antibacterial activity and the synergy of the sanguisorbigenin (SGB) from the dried root of Sanguisorba officinalis L. combined with β-lactam antibiotics against methicillin-resistant Staphylococcus aureus. A total of six strains of reference strain and clinical isolates were used to determine the antibacterial activity using a broth microdilution assay, and the synergistic effects were determined using a checkerboard assay. To analyse the mechanism of synergy, we conducted the level of penicillin-binding protein 2a by western blot. In addition, quantitative RT-PCR was performed to analyse the mecA gene expression. The minimal inhibitory concentration values of SGB against six strains of S. aureus were in the range of 12·5-50μgml(-1) , and there were synergy, or partial synergy effects when SGB was combined with antibiotics. Furthermore, when treated with SGB, the level of penicillin-binding protein 2a and the expression of the mecA gene was reduced significantly. In conclusion, this study demonstrated that SGB is a potential natural antibacterial agent against methicillin-resistant S. aureus that represents a considerable burden on the healthcare system worldwide, and may an exceptionally modulator of β-lactam antibiotics.
作者：
Deepa, Ponnuvel;Bae, Ho Jung;Park, Hyeon-Bae;Kim, So-Yeon;Choi, Ji Woong;...
期刊：
Journal of Ethnopharmacology,2020年253:112651- ISSN：0378-8741
通讯作者：
Park, Se Jin;Ryu, Jong Hoon
作者机构：
[Kim, So-Yeon; Deepa, Ponnuvel; Park, Se Jin; Park, Hyeon-Bae] Kangwon Natl Univ, Sch Nat Resources & Environm Sci, Chunchon, South Korea.;[Ryu, Jong Hoon; Bae, Ho Jung] Kyung Hee Univ, Dept Life & Nanopharmaceut Sci, Seoul, South Korea.;[Ryu, Jong Hoon] Kyung Hee Univ, Coll Pharm, Dept Oriental Pharmaceut Sci, Seoul, South Korea.;[Liu, Xiang-Qian] Hunan Univ Chinese Med, Sch Pharm, Changsha, Peoples R China.;[Choi, Ji Woong] Gachon Univ, Coll Pharm, Lab Neuropharmacol, Incheon, South Korea.
通讯机构：
[Park, Se Jin; Ryu, Jong Hoon] K;Kangwon Natl Univ, Sch Nat Resources & Environm Sci, Chunchon, South Korea.;Kyung Hee Univ, Coll Pharm, Dept Oriental Pharmaceut Sci, Seoul, South Korea.
关键词：
Dracocephalum moldavica;Memory impairment;Alzheimer's disease;Scopolamine;Extracellular signal regulated kinase;cAMP response element-binding protein
作者机构：
[刘向前; 肖瑾] School of Pharmacy, Hunan University of Chinese Medicine, Changsha, 410208, China;[李小军] College of Pharmaceutical Sciences, Gannan Medical University, Ganzhou, 341000, China;[黄建军] Department of Clinical Biochemistry, Xiangya Medical College, Central South University, Changsha, 410013, China;[Ko S.-K.] Department of Oriental Medical Food & Nutrition, Semyung University, Jecheon, 27136, South Korea;[Yook C.-S.] College of Pharmacy, Kyung Hee University, Seoul, 130701, South Korea
作者机构：
[Li, Xiao-Jun] Gannan Med Univ, Coll Pharmaceut Sci, Ganzhou 341000, Jiangxi, Peoples R China.;[Oh, Hyuncheol; Kim, Kwan-Woo; Li, Xiao-Jun; Kim, Dong-Cheol; Kim, Youn-Chul] Wonkwang Univ, Coll Pharm, Inst Pharmaceut Res & Dev, Lksan 54538, South Korea.;[Liu, Xiang-Qian] Hunan Univ Chinese Med, Sch Pharm, Changsha 410208, Hunan, Peoples R China.;[Oh, Hyuncheol; Kim, Kwan-Woo; Kim, Dong-Cheol; Kim, Youn-Chul] Wonkwang Univ, Hanbang Cardio Renal Syndrome Res Ctr, Iksan 54538, South Korea.;[Kim, Youn-Chul] Wonkwang Univ, Coll Pharm, Iksan 54538, South Korea.
通讯机构：
[Liu, Xiang-Qian] H;[Kim, Youn-Chul] W;Hunan Univ Chinese Med, Sch Pharm, Changsha 410208, Hunan, Peoples R China.;Wonkwang Univ, Coll Pharm, Iksan 54538, South Korea.
关键词：
Eleutherococcus henryi;Araliaceae;monoterpenoid glycosides;eleuhenryiside A;eleuhenryiside B;eleuhenryiside C
摘要：
The phytochemical investigation on the fruits of Eleutherococcus henryi (Araliaceae) resulted in the discovery of three novel monoterpene glycosides, eleuhenryiside A (1), eleuhenryiside B (2), and eleuhenryiside C (3), as well as a known lignan, (-)-kobusin (4). Their chemical structures were elucidated by mass, 1 D- and 2 D-NMR spectroscopy. The chemical structures of new compounds 1-3 were determined to be (2E,6R)-6-hydroxy-2,6-dimethyl-2,7-octadien-1-yl-(6'-O-acetyl)-O-beta-glucopyranoside, (2Z,6R)-6-hydroxy-2,6-dimethyl-2,7-octadien-1-yl-(6'-O-acetyl)-O-beta-glucopyranoside, and (-)-(4 R)-4,7-dihydroxy-1-menthene 7-O-beta-glucopyranoside, respectively. The anti-neuroinflammatory and anti-inflammatory activities of these compounds were evaluated with LPS-stimulated BV2 microglia and RAW264.7 macrophage, respectively. The results showed that new compounds 1 and 3 have inhibitory effects of NO production with IC50 values of 32.50 +/- 1.60 and 3.54 +/- 0.20 mu M in LPS-stimulated BV2 microglia. Also, (-)-kobusin (4) has abilities to inhibit NO production with the IC50 values of 14.25 +/- 2.69 and 36.35 +/- 6.27 mu M in BV2 and RAW264.7 cells, respectively, which indicated that it may possess the potential anti-neuroinflammatory and anti-inflammatory activities. [GRAPHICS] .
作者机构：
[Kee, Ji-Ye; Jeon, Hee-Dong; Hong, Seung-Heon; Mun, Jeong-Geon; Han, Yo-Han; Park, Seong-Hwan] Wonkwang Univ, Wonkwang Oriental Med Res Inst, Coll Pharm, Dept Oriental Pharm, 460 Iksandae Ro, Iksan 54538, Jeonbuk, South Korea.;[Park, Seong-Hwan] Korea Inst Oriental Med, Clin Med Div, Daejeon, South Korea.;[Park, Jinbong] Kyung Hee Univ, Coll Korean Med, Dept Pharmacol, 26 Kyungheedae Ro, Seoul 02447, South Korea.;[Zou, Qin-Peng] Changsha Broad Ocean Biosci & Tech Co Ltd, Changsha 410205, Hunan, Peoples R China.;[Liu, Xiang-Qian] Hunan Univ Chinese Med, Sch Pharm, Changsha 410208, Hunan, Peoples R China.
通讯机构：
[Hong, Seung-Heon] W;Wonkwang Univ, Wonkwang Oriental Med Res Inst, Coll Pharm, Dept Oriental Pharm, 460 Iksandae Ro, Iksan 54538, Jeonbuk, South Korea.
关键词：
3T3-L1;AMPK;Cassiae tora seed;Human adipose mesenchymal stem cells;Rubrofusarin-6-beta-gentiobioside;mTOR
摘要：
BACKGROUND: Although rubrofusarin-6-beta-gentiobioside (RFG), which is a component of Cassiae tora seed, could likely regulate hyperlipidemia, its anti-obesity effect and related mechanism have not been elucidated. PURPOSE: The aim of this study was to examine whether RFG can ameliorate obesity and the mechanism of lipid accumulation regulated by RFG. STUDY DESIGN: In in vitro experiments, we confirmed the anti-adipogenic effect of RFG using 3T3-L1 cells and human adipose mesenchymal stem cells (hAMSCs). To confirm the anti-obesity effect, High-Fat Diet (HFD)-induced obese mice were selected as a model. METHODS: We investigated anti-adipogenic effects of RFG using MTS assay, Oil Red O Staining, real-time RT-PCR, western blot analysis, and immunofluorescence staining. The anti-obesity effect of RFG was confirmed in HFD-induced mice model using hematoxylin and eosin staining and serum analysis. RESULTS: RFG inhibited lipid accumulation in 3T3-L1 cells and hAMSCs by reducing expression of mammalian targets of rapamycin (mTOR), peroxisome proliferator-activated receptor (PPAR)gamma, and CCAAT-enhancer binding protein (C/EBP)alpha. RFG phosphorylated AMP-activated protein kinase (AMPK) in a liver kinase B (LKB) 1-independent manner. Moreover, the anti-adipogenic effect of RFG was blocked by AMPK inhibitor. These results suggest that RFG inhibits lipid accumulation via AMPK signaling. Furthermore, RFG reduced the body weight, size of epididymal white adipose tissue (eWAT), and fatty liver in the mice. RFG also suppressed levels of adipogenic factors PPARgamma, C/EBPalpha, FAS, LPL, and aP2) by activating AMPK in the eWAT and liver. CONCLUSION: RFG can ameliorate obesity, and thus, could be used as a therapeutic agent for treating obesity.
关键词：
high-mobility group box 1;lupane-type triterpenoid saponins;Acanthopanax gracilistylus;tumor necrosis factor-alpha;interleukin-1 beta;nuclear factor-kappa B
摘要：
Acanthopanax gracilistylus (AGS) has long been used in traditional Chinese medicine for the treatment of various inflammatory diseases. 3-O-beta-D-glucopyranosyl 3 alpha, 11 alpha-dihydroxylup-20(29)-en-28-oic acid, acantrifoside A, acankoreoside D, acankoreoside B and acankoreoside A are major lupane-type triterpenoid saponins derived from AGS. In the present study, these five saponins were isolated from AGS by chromatography and their anti-inflammatory activities were investigated in lipopolysaccharide (LPS)-treated RAW264.7 macrophages. Cell viability was evaluated by MTT assay. Tumor necrosis factor (TNF)-alpha, interleukin (IL)-1 beta and NF-kappa B p65 were measured by ELISA. The gene expression levels of TNF-alpha and IL-1 beta was detected by reversetranscription polymerase chain reaction. And high-mobility group box 1 (HMGB1) were analyzed by western blotting. The results demonstrated that these five saponins significantly suppressed LPS-induced expression of TNF-alpha and IL-1 beta at the mRNA and protein level in RAW264.7 cells. Further analysis revealed that acankoreoside A and acankoreoside B were able to reduce the secretion of HMGB1 and NF-kappa B activity induced by LPS in RAW264.7 macrophages. Taken together, these results suggested that the anti-inflammatory activity of AGS-derived saponins may be associated with the downregulation of TNF-alpha and IL-1 beta, and the 'late-phase' proinflammatory cytokine HMGB1, via negative regulation of the NF-kappa B pathway in RAW264.7 cells.
作者：
Seo, Yun-Soo;Lee, Seok-Jeon;Li, Zhi;Kang, Ok-Hwa;Kong, Ryong;...
期刊：
Molecular Medicine Reports,2017年16(1):857-864 ISSN：1791-2997
通讯作者：
Kwon, Dong-Yeul;Liu, Xiangqian
作者机构：
[Zhou, Tian; Kong, Ryong; Kim, Sang-A; Kang, Ok-Hwa; Kwon, Dong-Yeul; Seo, Yun-Soo] Wonkwang Univ, Wonkwang Oriental Medicines Res Inst, Coll Pharm, Dept Oriental Pharm, 460 Iksandae Ro, Iksan 570749, Jeollabuk, South Korea.;[Lee, Seok-Jeon] Wonkwang Univ, Dept Med 3, Profess Grad Sch Oriental Med, Iksan 570749, Jeollabuk, South Korea.;[Liu, Xiangqian; Li, Zhi] Hunan Univ Chinese Med, Sch Pharm, 300 Xueshi Rd, Changsha 410208, Hunan, Peoples R China.
通讯机构：
[Kwon, Dong-Yeul] W;[Liu, Xiangqian] H;Wonkwang Univ, Wonkwang Oriental Medicines Res Inst, Coll Pharm, Dept Oriental Pharm, 460 Iksandae Ro, Iksan 570749, Jeollabuk, South Korea.;Hunan Univ Chinese Med, Sch Pharm, 300 Xueshi Rd, Changsha 410208, Hunan, Peoples R China.
关键词：
araliasaponin II;anti-inflammatory;Toll-like receptor 4;nuclear factor-kappa B
摘要：
Araliasaponin II (AS II) is a bioactive compound isolated from Acanthopanax henryi (Oliv.) Harms, a plant widely used in traditional oriental medicine. The present study investigated the anti-inflammatory effects of AS II using murine macrophages. The effects of AS II on inflammatory mediator and cytokine production in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells was evaluated. Nitric oxide (NO) and cytokine production were determined using the Griess reagent and an ELISA kit. The expression levels of cytokines, inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2) mRNA were examined by reverse transcription-quantitative polymerase chain reaction. The expression levels of iNOS, COX-2 and toll-like receptor (TLR)-4 protein were examined by western blotting. Translocation of nuclear factor-kappa B (NF-kappa B) and TLR-4 expression were visualized by immunofluorescence staining. AS II markedly inhibited the production of NO and prostaglandin E2, and reduced iNOS and COX-2 expression at the transcriptional and translational levels. AS II downregulated the expression of interleukin-6 and tumor necrosis factor-alpha at the protein and mRNA levels. Furthermore, pre-treatment with AS II significantly suppressed the TLR-4-NF-kappa B signaling pathway; this effect may be cause by AS II competing with LPS for binding to TLR-4 and subsequently inhibiting translocation of the NF-kappa B/p65 protein to the nucleus. The results suggested that the anti-inflammatory properties of AS II may result from inhibiting pro-inflammatory mediators by suppressing the initiation of the inflammatory response and inhibiting TLR-4-NF-kappa B signaling pathways.
作者机构：
[李芝; 张斌贝; 刘向前; 罗姣; 高大林] School of Pharmacy, Hunan University of Chinese Medicine, Changsha, 410208, China;[邹亲朋] Changsha Broad-Ocean Bio-Science & Technique Co., Ltd., Changsha, 410205, China
通讯机构：
[Liu, X.-Q.] S;School of Pharmacy, Hunan University of Chinese Medicine, Changsha, China
关键词：
糙叶五加;总黄酮;大孔吸附树脂;响应曲面设计法;纯化;单因素实验;吸附;洗脱
摘要：
目的研究优化糙叶五加叶总黄酮的大孔吸附树脂纯化工艺。方法以处理后的总黄酮的质量分数和总黄酮得率为考察指标,采用单因素实验结合响应曲面设计法进行纯化工艺优化研究。结果D101型大孔吸附树脂对糙叶五加叶总黄酮有较好的吸附和洗脱效果,其最佳的纯化工艺条件为色谱柱的径高比为1∶10,25.0 g D101大孔树脂的上样量为750 mg,溶剂体系为50%乙醇,洗脱体积流量为5 mL/min,洗脱体积为130 mL。在此最佳优化条件下,糙叶五加叶总黄酮的理论质量分数为75.69%,实际质量分数达到75.87%;总黄酮得率为30.13%。结论 D101型大孔吸附树脂宜于纯化糙叶五加叶中的总黄酮,且响应面法能有效地优化其纯化工艺。
