通讯机构：
[Yan, Jie] H;Hunan Univ Tradit Chinese Med, Inst Acupuncture & Moxibust, Changsha 410007, Hunan, Peoples R China.
关键词：
Electroacupuncture;Gastric histology;Gastric mucosal protection;Intestinal trefoilf factor;Relative particularity between stomach meridian of foot-yangming and stomach;Stomach meridian of Foot-yangming
作者机构：
[Yang, PC] Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Canada<&wdkj&>an adjunct Professor of Allergy Unit and Department of Otolaryngology, Shanxi Medical University, Taiyuan, China;[Liu, T; Wang, BQ; Zhang, TY] Department of Otolaryngology, Shanxi Medical University, the First Hospital, Taiyuan, China;[An, ZY] Department of Gastroenterology, Shanxi Medical University, the First Hospital, Taiyuan, China;[Zheng, PY] Department of Gastroenterology, Zhengzhou University, the Second Hospital, Zhengzhou, China;[Tian, DF] Department of Otolaryngology, Hunan University of Chinese Traditional Medicine, Changsha, China
通讯机构：
[Yang, PC ] ;McMaster Univ, Dept Pathol & Mol Med, Hamilton, ON, Canada.
摘要：
During clinical practice, we noticed that some patients with both ulcerative colitis (UC) and chronic rhinosinusitis (CRS) showed amelioration of UC after treatment of CRS. This study was designed to identify a possible association between CRS and UC. Thirty-two patients with both CRS and UC received treatment with functional endoscopic sinus surgery (FESS) for CRS. Clinical symptom scores for CRS and UC, as well as serum levels of anti-Staphylococcal enterotoxin B (SEB) were evaluated at week 0 and week 12. Sinus wash fluid SEB content was measured with enzyme-linked immunosorbent assay (ELISA). The surgically removed tissues were cultured to identify growth of Staphylococcus. aureus (S. aureus). Immunohistochemistry was employed to identify anti-SEB positive cells in the colonic mucosa. Colonic biopsies were obtained and incubated with SEB. Mast cell activation in the colonic mucosa in response to incubation with SEB was observed with electron microscopy and immunoassay. The clinical symptom scores of CRS and UC severe scores (UCSS) were significantly reduced in the UC-CRS patients after FESS. The number of cultured S. aureus colonies from the surgically removed sinus mucosa significantly correlated with the decrease in UCSS. High levels of SEB were detected in the sinus wash fluids of the patients with UC-CRS. Histamine and tryptase release was significantly higher in the culture supernate in the patients with UC-CRS than the patients with UC-only and normal controls. Anti-SEB positive cells were located in the colonic mucosa. The pathogenesis of UC in some patients may be associated with their pre-existing CRS by a mechanism of swallowing sinusitis-derived SEB. We speculate that SEB initiates inappropriate immune reactions and inflammation in the colonic mucosa that further progresses to UC.
通讯机构：
[Yan, Jie] H;Hunan Coll Tradit Chinese Med, Dept Acupuncture & Moxibust, Changsha 410007, Hunan, Peoples R China.
关键词：
Gastric mucosal injury/acupuncture effects;MMCSV;Relationship between Meridian and Viscus
摘要：
AIM: To explore the regularity of multi-meridians controlling a same viscus (MMCSV). METHODS: The rabbit gastric ulcer model was established by ethanol intragastric instillation. Fifty-six rabbits were randomly divided into normal group, model group (MG), model plus acupuncture at Foot Yangming Meridian group (YMG), model plus acupuncture at Foot Taiyin Meridian group (TYG), model plus acupuncture at Foot Shaoyang Meridian group (SYG), model plus acupuncture at Foot Jueyin Meridian group (JYG), model plus acupuncture at Foot Taiyang Meridian group (TYMG), with eight rabbits in each group. Gastric mucosal nitric oxide (NO) and nitric oxide synthase (NOS) were assayed by the nitric acid reductase method, and prostaglandin E2 (PGE2) and epidermal growth factor (EGF) were measured by radioimmunoassay. The comprehensive effects were analyzed by weighing method. RESULTS: Compared to MG, SYG, JYG and TYMG, the rabbits gastric mucosal injury index (GMII) reduced very significantly in YMG (P < 0.01). Compared to MG, the GMII also reduced significantly in TYG (P < 0.05). NO, NOS, PGE2 and EGF increased very significantly in YMG (P < 0.01). The EGF in YMG also increased significantly than that in TYG compared to those in MG, SYG, JYG and TYMG (P < 0.05). The PGE2 and EGF also increased very significantly in TYG than those in MG, JYG and TYMG (P < 0.01). While compared to SYG, the NOS increased significantly in TYG (P < 0.05). NOS was the highest in YMG (P < 0.01), and was higher in TYG than in MG (P < 0.01). CONCLUSION: MMCSV is common. The Foot Yangming Meridian is most closely related to the stomach, followed by Foot Taiyin Meridian, Foot Shaoyang Meridian and Foot Jueyin Meridian. Foot Taiyang Meridian has no correlation with the stomach. (C) 2005 The WJG Press and Elsevier Inc. All rights reserved.
关键词：
c-fos;nucleus of the solitary tract;acupuncture;facial acupoints;gastric distension
摘要：
Clinical practice has shown that acupuncture at facial acupoints has curative effects on some visceral diseases (especially gastrointestinal diseases). However, the physiological basis has not been clarified yet. In the present study, expression of c-fos in the nucleus of the solitary tract (NTS) of rats following gastric distension and electroacupuncture (EA) at Yangbai (GB14) and Sibai (ST2) as well as Jiache (ST6) acupoints was observed by using immunohistochemistry technique. After EA at the three facial acupoints, c-fos immunoreactive (c-fos-IR) neurons were mainly distributed in the medial (mNTS) and intermediate subnucleus of the NTS, and a few were scatteredly distributed in the dorsalmedial and commissural subnucleus of the NTS. Furthermore, there is difference in the number of c-fos-IR neurons in the mNTS following EA at the three facial acupoints. The number in the EA at ST2 and GB14 group is the highest and the lowest, respectively. Gastric distension induces obviously the expression of c-fos, which is mainly confined in the mNTS. The results suggest that the noxious visceral and somatic afferent information from the stomach and face may converge in the mNTS, which may be involved in the effect of EA at facial acupoints on the gastrointestinal pain.
作者机构：
[He, ST] Cent S Univ, Xiangya Hosp, Inst Combined Tradit Chinese & Western Med, Changsha 410008, Hunan Province, Peoples R China.;Hunan Province Acad Tradit Chinese Med, Changsha 410006, Peoples R China.;Hunan Coll Trad Chinese Med, Dept Microbiol, Changsha 410007, Peoples R China.
通讯机构：
[He, ST] C;Cent S Univ, Xiangya Hosp, Inst Combined Tradit Chinese & Western Med, Changsha 410008, Hunan Province, Peoples R China.
摘要：
AIM: To observe the effects of Qiwei Baizhu Powder (QWBZP) on rotaviral gastroenteritis in children and in animal models. METHODS: Enrolled patients were divided into two groups, and one group was treated with oral rehydration solution (ORS) and the other treated with oral liquid of QWBZP. Neonate mice were orally infected with 50 microL rotavirus suspension (4 X 10(8) PFU/mL) and treated with ORS or oral liquid of QWBZP, respectively. RESULTS: Eighty-three cases of rotaviral gastroenteritis treated with QWBZP revealed a better efficacy than that treated with ORS (X(2)=10.87, P < 0.05). The contents of sodium and glucose as well as number of patients with positive human rotavirus antigen in stool in QWBZP group were all less than that in ORS group. In animal models, QWBZP was found effective in treating rotavirus gastroenteritis in neonate NIH mice, as compared with control groups. In QWBZP group, the mortality of infected mice was decreased by 73.3%, the body weight of infected mice was increased, the contents of sodium and glucose as well as number of mice with positive rotavirus antigen in feces were significantly reduced, and the pathological changes such as damage of small intestinal mucosa and villi were also obviously alleviated. CONCLUSION: QWBZP has effects on improving the absorptive function of small intestine, shortening the duration of diarrhea and rotavirus shedding from stool and alleviating the pathological changes of small intestine induced by rotavirus.
摘要：
A novel flavanone glycoside has been isolated from the Chinese crude drug ''mu tong'' which is used in Hunan province, China. The structure of this new natural product is identified as 5,4'-dihydroxy-3'-methoxyflavanone-7-(6''-O-beta-L-rhamnopyranosyl)-beta-D-glucopyranoside by H-1, C-13 NMR, COSY, H-1, C-13 HETCOR, LOCOR and 1D NOE-difference spectra.
