摘要：
The pK(a) values of a series of fluoroalkanesulfonylamides were measured by potentiometric titration. Different kinds of alkyl halides and tosylates were employed to investigate the nucleophilicity of fluoroalkanesulfonylamides. Fluoroalkanesulfonylamides with longer fluoroalkyl chain have weaker nucleophilicity. (C) 2010 Elsevier B.V. All rights reserved,
摘要：
Although draft genome sequences of two of the major human schistosomes, Schistosoma japonicum and Schistosoma mansoni are available, the structures and characteristics of most genes and the influence of exogenous genes on the metabolism of schistosomes remain uncharacterized. Furthermore, which functional genomics approaches will be tractable for schistosomes are not yet apparent. Here, the vesicular stomatitis virus glycoprotein (VSVG)-pseudotyped pantropic retroviral vector pBABE-puro was modified to incorporate the human telomerase reverse transcriptase gene (hTERT) as a reporter, under the control of the retroviral long terminal repeat (LTR). Pseudotyped virions were employed to transduce S. japonicum to investigate the utility of retrovirus-mediated transgenesis of S japonicum and the activity of human telomerase reverse transcriptase as a reporter transgene in schistosomes. Schistosomules perfused from experimentally infected rabbits were cultured for 6 days after exposure to the virions after which genomic DNAs from virus exposed and control worms were extracted. Analysis of RNA from transduced parasites and immunohistochemistry of thin parasite sections revealed expression of hTERT in the transduced worms. Expression of hTERT was also confirmed by immunoblot analysis. These findings indicated that S. japonicum could be effectively transduced by VSVG-pseudotyped retrovirus carrying the hTERT gene. Given the potential of hTERT to aid in derivation of immortalized cells, these findings suggest that this pantropic retroviral approach can be employed to transduce cells from specific tissues and organs of schistosomes to investigate the influence of transgene hTERT on growth and proliferation of schistosome cells. (C) 2010 Elsevier B.V. All rights reserved.
作者机构：
[Zhu Bing-Yang; Luo Di-Xian; Xu Can-Xin; Wang Chun; Gao Zhi-Ping; Liao Duan-Fang] Univ S China, Life Sci Res Ctr, Inst Pharm & Pharmacol, Hengyang 421001, Peoples R China.;[Liao Duan-Fang] Hunan Univ Chinese Med, Dept Tradit Chinese Diagnost, Changsha 410208, Hunan, Peoples R China.
通讯机构：
[Liao Duan-Fang] U;Univ S China, Life Sci Res Ctr, Inst Pharm & Pharmacol, Hengyang 421001, Peoples R China.
摘要：
Arylfluoroalkylsulfoxides were successfully synthesized in one-pot when fluoroalkylsulfinate reacted with benzene and triffic anhydride in triflic acid and dichloromethane as the medium. The characteristics of their F-19 NMR spectra were examined and analyzed for these structures. Electronic and steric effects of substituents at alpha- or beta-position were revealed to be the main cause of the anomalous behavior of their chemical shifts and coupling constants. Interactions between arylfluoroalkylsulfoxides and solvents were also investigated and discussed. (C) 2009 Elsevier B.V. All rights reserved.
作者机构：
[Li, Hua; Chen, Bei-Yang] Hunan Univ Tradit Chinese Med, Dept Histol & Embryol, Changsha, Hunan, Peoples R China.;[Deng, Chang-Qing; Zhang, Shu-Ping; Liang, Yan] Hunan Univ Tradit Chinese Med, Lab Pathophysiol, Changsha, Hunan, Peoples R China.;[Li, Hua; Luo, Xue-Gang] Cent S Univ, Xiangya Sch Med, Dept Anat & Neurobiol, Changsha, Hunan, Peoples R China.
通讯机构：
[Deng, Chang-Qing] H;Hunan Univ Tradit Chinese Med, Lab Pathophysiol, Changsha, Hunan, Peoples R China.
关键词：
Total saponins of Panax notoginseng;Cerebral ischemia-reperfusion;TUNEL;Caspase-1;Caspase-3;Caspase-8
摘要：
Ethnopharmacological relevance: Total saponins of Panax notoginseng (TSPN), main constituents extracted from Panax Notoginseng, a highly valued traditional Chinese medicine, have been shown to be an effective agent on cerebral infarction. Aim of the study: The effects of TSPN on apoptosis and expressions of caspase-1, caspase-3 and caspase-8 were studied after cerebral ischemia for 2 h followed by reperfusion for 46 h in rats. Materials and methods: Rats were subjected to transient middle cerebral artery occlusion (MCAO) model using the intraluminal thread. TSPN was administered intraperitoneally at 5 min before and 12 h, 24 h and 36 h after MCAO, respectively. Results: TSPN (at the dose of 25 mg/kg) significantly attenuated TUNEL-positive cells and reduced the expression of caspase-1 and caspase-3 compared to the model group, while it had no obvious effect on the expression of caspase-8. Conclusions: The neuroprotective effect of TSPN on focal ischemia may be related to inhibition of apoptosis and caspases activation. (c) 2008 Elsevier Ireland Ltd. All rights reserved.
摘要：
A new stilbene glycoside, 5-methylresveratrol-3,4'-O-beta-D-diglucopyranoside (1), was isolated from the n-butanol fraction of the rhizomes of Veratrum dahuricum, together with five known stilbenoids: resveratrol-3-O-beta-D-glycoside (2), 4'-methylresveratrol-3-O-beta-D-glycoside (3), oxyresveratrol-4'-O-beta-D-glycoside (4), oxyresveratrol-3-O-beta-D-glycoside (5), and oxyresveratrol-3,4'-O-beta-D-diglycoside (6), and found for the first time in the investigated plant. The structures of six isolates were identified on the basis of 1D and 2D NMR data. Compounds 1-6 showed platelet aggregation inhibition, and compound 1 had an IC50 value of 383.6 mu M against platelet aggregation induced by AA.
关键词：
depression;regulating the liver;acupuncture therapy;investigation
摘要：
OBJECTIVE: To observe therapeutic effect of acupuncture for regulating the liver on depressive neurosis. METHODS: In a multi-center randomized controlled trial, 440 patients were divided into 3 groups: Acupuncture group for regulating the liver (Acup., 176 cases) was treated by acupuncture at Siguan Points, i.e., bilateral Hegu (LI 4) and Taichong (LR 3), Baihui (GV 20) and Yintang (EX-HN3) plus ear-acupuncture, Prozac group (P., 176 cases) by oral administration of Prozac, and Non-acupoint needling group (NAN, 88 cases) by acupuncture at non-acupoints as acupuncture placebo. Self-rating Depression Scale (SDS) was examined before treatment, and one month, two and three months after treatment respectively to evaluate therapeutic effect, and Rating Scale for Side Effects (SERS) was used to evaluate the safety. RESULTS: After one month of treatment, SDS scores in Acup. Group were significantly lower than that in P. Group (P < 0.05) and than that in NAN Group (P < 0.01), and SDS scores in P. Group were lower than that in NAN Group (P < 0.05), showing the SDS scores in Acup. Group < P. Group < NAN Group. After 2 months of treatment, SDS scores in Acup. Group were also significantly lower than that in P. Group (P < 0.01) and than that in NAN Group (P < 0.01), and SDS scores in P. Group were also lower than that in NAN Group (P < 0.05), showing the SDS scores in Acup. Group <P. Group <NAN Group. After 3 months of treatment, SDS scores in Acup. Group were also significantly lower than that in P. Group (P < 0.01) and than that in NAN Group (P < 0.01), and SDS scores in P. Group were also lower than that in NAN Group (P < 0.01), showing the SDS score in Acup. Group < P. Group < NAN Group. After treatment, SERS scores were 0.16 +/- 0.95, 6.51 +/- 5.09 and 0.23 +/- 1.36 in Acup. Group, P. Group and NAN Group respectively. A significant difference existed between Acup. Group and P. Group (P < 0.05), but no significant difference between Acup. Group and NAN Group (P > 0.05), showing the SERS scores in Acup. Group < NAN Group < P. Group. No side effect was found in Acup. and NAN groups. CONCLUSION: The therapeutic effect of acupuncture on depressive neurosis is better than or similar to that of Prozac but with less side effect.
作者机构：
[Wu Yan-shu; Zhang Qian; Jin Cheng; Xiao Xiao-he; Yan Dan] 302 Mil Hosp China, PLA Inst Chinese Mat Med, Beijing 100039, Peoples R China.;[Wu Yan-shu; Zhang Qian; Wang Jian] Chengdu Univ Tradit Chinese Med, Coll Pharm, Chengdu 611137, Peoples R China.;[Jiao Yi] Hunan Univ Tradit Chinese Med, Coll Pharm, Changsha 410002, Hunan, Peoples R China.;[Du Xiao-xi] State Food & Drug Adm, Ctr Drug Evaluat, Beijing 100038, Peoples R China.
通讯机构：
[Xiao Xiao-he] 3;302 Mil Hosp China, PLA Inst Chinese Mat Med, Beijing 100039, Peoples R China.
作者机构：
[Yang, Xu-Guang; Ma, Ting-Ting; Li, Ying; Liang, Fan-Rong; Yu, Shu-Yuan; Tian, Xiao-Ping] Chengdu Univ Tradit Chinese Med, Chengdu 610075, Sichuan, Peoples R China.;[Sun, Guo-Lie] Hubei Coll Tradit Chinese Med, Wuhan 430065, Hubei, Peoples R China.;[Yan, Jie; Chang, Xiao-Rong] Hunan Univ Tradit Chinese Med, Changsha 410007, Hunan, Peoples R China.
通讯机构：
[Liang, Fan-Rong] C;Chengdu Univ Tradit Chinese Med, Chengdu 610075, Sichuan, Peoples R China.
关键词：
Nepean Dyspepsia Index;Functional dyspepsia;Health-related quality of life;Validation studies;Questionaires
摘要：
AIM: To assess the reliability and validity of the translated version of Nepean Dyspepsia Index (NDI) in Chinese patients with documented functional dyspepsia (FD).METHODS: The translation process included forward translation, back translation, pretest and cross-cultural adaptation. Reliability and validity of the translated version were examined by asking 300 subjects to complete the Chinese version of the NDI. The mean age of subjects was 39.24 years and 68.7% of the subjects were women. Internal consistency analysis with Cronbach's α was performed to test the reliability. Correlation analysis was used to assess the content validity. Factor analysis and structural equation models were used to assess the construct validity.RESULTS: The Cronbach's α coefficients ranged 0.833-0.960, well above the acceptable level of 0.70. Correlation analysis showed that each item had a strong correlation with the corresponding domain, but a weak correlation with other domains. Confirmatory factor analysis indicated that the comparative fit index was 0.94, higher than the acceptable level of 0.90.CONCLUSION: The Chinese version of the NDI is a reliable and valid scale for measuring health-related quality of life and disease severity in Chinese patients with FD.
摘要：
Objective: To probe into the mechanisms of electroacupuncture (EA) at Shangjuxu (ST 37) for treatment of the ulcerative colitis (UC). Methods: Forty male Wistar rats were randomly divided into 4 groups: a normal group, a model group, a Shangjuxu group and a non-acupoint group, 10 rats in each group. The UC rat model was made with enema of trinitro-benzenesulfonic acid (TNBSA), and the changes of interleukin-1 beta (IL-1 beta) and interleukin-4 (IL-4) contents after EA at Shangjuxu (ST 37) were observed. Results: EA at Shangjuxu (ST 37) could significantly decrease the IL-1 beta content and increase the IL-4 content in the colic tissues of the UC rats with significant differences as compared with the model group and the non-acupoint group (P<0.05 or P<0.01). Conclusion: The mechanisms of EA at Shangjuxu (ST 37) for treatment of the UC rats is possibly related with the decrease of IL-1 beta, a inflammation-promoting cytokine, and the increase of IL-4, a anti-inflammatory cytokine.
作者机构：
[Cai Guangming; Shen Qian; Wu Na; Zhao Yanling; Wang Feng] Inst Chinese Mat Med, Beijing 100039, Peoples R China.;[Shen Qian; Wu Na; He Qun] Hunan Univ Tradit Chinese Med, Changsha 410208, Hunan, Peoples R China.;[Wu Na] Inst Chinese Mat Med, 302 Hosp PLA, Beijing 100039, Peoples R China.
通讯机构：
[Wu Na] I;Inst Chinese Mat Med, 302 Hosp PLA, Beijing 100039, Peoples R China.
作者机构：
[Ren YongShen; Wang Jiabo; Zhao HaiPing; Yan Dan; Xiao XiaoHe; Zhou CanPing; Li HanBing; Zhao YanLing] 302 Mil Hosp, China Mil Inst Chinese Mat Med, Beijing 100039, Peoples R China.;[Ren YongShen; Zhao HaiPing; Li HanBing] Chengdu Univ Tradit Chinese Med, Chengdu 610075, Peoples R China.;[Zhou CanPing] Hunan Univ Chinese Med, Changsha 410208, Hunan, Peoples R China.
通讯机构：
[Xiao XiaoHe] 3;302 Mil Hosp, China Mil Inst Chinese Mat Med, Beijing 100039, Peoples R China.
关键词：
Rhubarb;Strobal;Cold/Hot nature of Chinese medicines;cold/hot plate differentiating assay
摘要：
In this study, objective differences between the Cold (Han) and Hot (Re) nature of traditional Chinese medicines, e.g. Strobal and Rhubarb, are determined by using a cold/hot plate differentiation technology. A novel, self-designed cold/hot plate differentiating instrument, with methodological study, was used to investigate the intervention of Strobal and Rhubarb on the temperature tropism of mice. Compared with the ICR and BALB/c mice, it was found that KM mice on the cold/hot plate were more sensitive to the change of temperature, within the tolerant temperature range of 15-40 degrees C. The temperature tropism behavior of mice is influenced by treatment with Rhubarb and Strobal, as is the activity of ATPase in liver tissue. These trends are consistent with the definition of the Cold/Hot nature of Chinese medicines based on traditional Chinese medicinal theory. This study showed that the differences of the Cold/Hot nature of traditional Chinese medicines. might be objectively represented by the temperature tropism of animal by means of cold/hot differentiating assay.
作者机构：
[Zhang XueRu; Ren YongShen; Wang JiaBo; Zhao HaiPing; Xiao XiaoHe; Zhou CanPing; Zhao YanLing] 302 Mil Hosp China, China Mil Inst Chinese Meteria Med, Beijing 100039, Peoples R China.;[Zhang XueRu; Xia XinHua; Zhou CanPing] Hunan Univ Chinese Med, Coll Pharmaceut Sci, Changsha 410208, Hunan, Peoples R China.
通讯机构：
[Xiao XiaoHe] 3;302 Mil Hosp China, China Mil Inst Chinese Meteria Med, Beijing 100039, Peoples R China.
关键词：
"Cold" and " Hot" natures of Chinese medicine;evaluation;temperature tropism;ethology;drug process;Coptis chinensis Franch
摘要：
The description and differentiation of the so-called "Cold" and "Hot" natures, the primary "Drug Naure" of Chinese medicine, is the focus of theoretical research. In this study, the divergency between the "Cold" and the "Hot" natures was investigated through examining the temperature tropism of mice affected by Coptis chinensis Franch and its processed materials by using a cold/hot plate differentiating technology. After exposure to C. chinensis Franch, the macroscopic behavioral index of the remaining rate (RR) on a warm pad (40 A degrees C) significantly increased (P < 0.05), suggesting the enhancement of Hot tropism. The internal indexes of adenosine triphosphatase (ATPase) activity and oxygen consuming volume decreased significantly (P < 0.05), suggesting the decapability of energy metabolism. This external behavior of Hot tropism might reflect the internal Cold nature of C. chinensis Franch. However, the processed materials of C. chinensis Franch exhibited a different Cold nature in temperature tropism compared with crude C. chinensis Franch (CC): the Cold nature of bile-processed C. chinensis Franch (BC) enhanced while the ginger-processed C. chinensis Franch (GC) changed inversely. The changing sequence was consistent with the theoretical prognostication. It is indicated that the external Cold & Hot natures of Chinese medicine may possibly reflect in an ethological way for the changes of animal's temperature tropism which might be internally regulated by the body's energy metabolism.
期刊：
Journal of Zhejiang University. Science. B,2009年10(3):172-179 ISSN：1673-1581
通讯作者：
He, Ying-chun
作者机构：
[Tian, Dao-fa; He, Ying-chun] Chinese Med Univ Hunan, Fac Integrat Med, Changsha 410007, Peoples R China.;[Lu, Fang-guo] Chinese Med Univ Hunan, Fac Basic Med, Changsha 410007, Peoples R China.;[Tang, Fa-qing] Cent S Univ, Dept Clin Lab, Xiangya Hosp 1, Changsha 410008, Peoples R China.
通讯机构：
[He, Ying-chun] C;Chinese Med Univ Hunan, Fac Integrat Med, Changsha 410007, Peoples R China.
摘要：
OBJECTIVE: To investigate the enhancive effect of N,N'-dinitrosopiperazine (DNP) on induced carcinogenesis in nasal and/or nasopharyngeal epithelia among TgN(p53mt-LMP1)/HT transgenic mice to examine the underlying mechanism for the development of nasopharyngeal carcinoma (NPC). METHODS: TgN(p53mt-LMP1)/HT transgenic mice and the same strain of C(57)BL/6J wild-type mice both at the age of 5 months were randomly divided into 2 groups in parallel, respectively, i.e., TgN(p53mt-LMP1)/HT cancerous lesion-inducing group (TI), TgN(p53mt-LMP1)/HT control group (TC), C57BL/6J cancerous lesion-inducing group (CI), and C57BL/6J control group (CC). TI and CI mice were treated only with DNP for 16 weeks, twice each week, while TC and CC mice were given the same volume of saline as controls. At the end of treatment, animals were sacrificed to collect epithelial tissue samples from nasal cavity and nasopharynx for pathohistological evaluation by haematoxylin and eosin (HE) staining and for determination on the expression of TRAF2, c-Jun, and p16 by immunohistochemistry. RESULTS: Atypical hyperplasia was more significant in the samples of TI than in those of TC, CI, and CC, with the rates of lesions being 90%, 10%, 0, and 0 (P<0.01) respectively, though DNP was used alone in a much shortened inducing period at less dosage and without the use of carcinogenic promoter 12-O-tetradecanoylphorbol-13-acetate as usual. The expressions of tumor necrosis factor (TNF) receptor-associated factor 2 (TRAF2) and c-Jun in these samples were significantly up-regulated in TI (P<0.01), while the expression of p16 was significantly lower in TI than in the other groups (P<0.01). CONCLUSION: TgN(p53mt-LMP1)/HT mice hold inherited constitutional defect in immune surveillance function, which can be aggravated by environmental carcinogens, such as DNP used even though in a much less strength. The enhanced carcinogenesis-inducing effect of DNP on TgN(p53mt-LMP1)/HT mice should be closely associated with abnormal signaling of activator protein-1 (AP-1) pathway, especially up-regulated expressions of TRAF2 and c-Jun, and down-regulated expression of p16.
