作者机构：
[Huang, D.; Yue, C. H.; Li, S. X.] Hunan Acad Tradit Chinese Med, Hunan Prov Key Lab Drug Res, Changsha 410013, Peoples R China.;[Huang, D.; Yue, C. H.; Li, S. X.] Hunan Univ Tradit Chinese Med, Changsha 410007, Peoples R China.;[Long, M.; Li, S. X.; Zhang, Z. G.] Hunan Agr Univ, Changsha 410128, Peoples R China.;[Long, M.] SUNY Stony Brook, Stony Brook, NY 11794 USA.;[Zhang, Z. G.] Hunan Normal Univ, Changsha 410081, Peoples R China.
会议名称：
第九届全国中药和天然药物学术研讨会
会议时间：
2007-12-01
会议地点：
南昌
会议论文集名称：
第九届全国中药和天然药物学术研讨会论文集
关键词：
叶表面结构;骨碎补;柚皮苷;山奈酚;补肾成份
摘要：
OBJECTIVE：To study the kidney tonic components of Rhizoma Drynariae.METHODS：The herbal material was extracted with 50％ ethanol.Silica gel and sephadex LH-20 coluuln chromatography isolated the chemical components using the Opossurn Kidney cell for the living model.The structures were identified on the basis of chemical and spectroscopic data.RESULTS.Six monocases were isolated from the EtOH extract of in Rhizomes of Drynaria fortunei J.Sm..Four structures were identified as naringin(I)，neocriocitrin(Ⅱ)，kaempferol(Ⅲ)，and naringenin-7-O-β-D-glucoside(Ⅳ)，respectively，on the basis of its spectral and physicochemical evidence.The kaempferol call promote the proliferation of the Opossum Kidney cell tested by Promega’B CellTiter96 assay in vitr0.Others are in identifying.CONCLUSION：Compound In and IV are firstly isolated from this plant，and the later may hefurther developed into a new effective drug for treatingm rural failure.
摘要：
Apelin is the endogenous ligand of the G protein-coupled receptor, APJ. Vascular smooth muscle cells express both apelin and APJ, which are important regulatory factors in the cardiovascular and nervous systems. Importantly, APJ is also involved in the pathogenesis if HIV-1 infection. We investigated whether vascular smooth muscle cell proliferation was regulated through an apelin-pERK1/2-cyclin D1 signal transduction pathway. Apelin-13 significantly stimulated vascular smooth muscle cell proliferation and increased cell cycle progression. Apelin-13 a decreased the proportion of cell in the G0/G1 phase while increasing the number of cells in S phase. Apelin-13 also increased the levels of cyclin D1, cyclin E and pERK1/2. Treatment of cells with the MEK inhibitor PD98059 attenuated the apelin-3-induced pERK1/2 activation. Similarly, treatment with PD98059 partially diminished the apelin-13-induced expression of cyclin D1 and vascular smooth muscle cell proliferation. Taken together, these data established that apelin-13 stimulates vascular smooth muscle cell proliferation by promoting the G1-S phase transition, and that this effect is mediated in part by an apelin-pERK1/2-cyclin D1 signal cascade.
摘要：
A dye intermediate, 1-amino-8-naphthol-3,6-disulfonic acid sodium (ANDS) was first used to selectively recognize Hg(II) in aqueous solutions with its fluorescence being strong quenched. The fluorescence quenching of ANDS was attributed to the formation of an inclusion complex between Hg(II) and ANDS by 2:1 complex ratio (K= 6.2 x 10(9)), which has been utilized as the basis of the fabrication of the Hg(II)-sensitive fluorescent chemosensor. The analytical performance characteristics of the proposed chemosensor were investigated. The sensor shows a linear response toward Hg(II) in the concentration range 2.9 x 10(-6) to 5.5 x 10(-5) M with a limit of detection of 5.3 x 10(-7) M, and a working pH range from 5.0 to 9.0. It shows excellent selectivity for Hg(II) over a large number of cations such as alkali, alkaline earth and transitional metal ions. The proposed method was utilized successfully for the detection of Hg2+ in water samples. (C) 2007 Elsevier B.V. All rights reserved.
摘要：
AIM: To study the effect of moxibustion on Zusanli or Liangmeng point on gastric mucosa injury in stress-induced ulcer rats and its correlation with the expression of heat shock protein 70 (HSP70). METHODS: Sixty healthy SD rats (30 males, 30 females) were divided into control group, injury model group, Zushanli point group, Liangmeng point group. Stress gastric ulcer model was induced by binding cold stress method. Gastric mucosa ulcer injury (UI) index was calculated by Guth method. Gastric mucosa blood flow (GMBF) was recorded with a biological signal analyzer. Protein content and gene expression in gastric mucosal HSP70 were detected by immunohistochemistry (IHC) and reverse transcription polymerase chain reaction (RT-PCR). Thiobarbital method was used to determine malondialdehyde (MDA) content. Gastric mucosal endothelin (ET) and prostaglandin E-2 (PGE(2)) were analyzed by radioimmunoassay. RESULTS: High gastric mucosal UI index, high HSP70 expression, low GMBF and PGF(2), elevated MDA and ET were observed in gastric mucosa of rats subjected to cold stress. Moxibustion on Zusanli or Liangmeng point decreased rat gastric mucosal UI index, MDA and ET Conversely, the expression of HSP70, GMBF, and PGE2 was elevated in gastric mucosa after pretreatment with moxibustion on Zusanli or Liangmeng point. The observed parameters were significantly different between Zusanli and Liangmeng points. CONCLUSION: Pretreatment with moxibustion on Zusanli or Liangmeng point protects gastric mucosa against stress injury. This protection is associated with the higher expression of HSP70 mRNA and protein, leading to release of PGE2 and inhibition of MDA and ET impairment of gastric mucosal index. (c) 2007 WJG. All rights reserved.
摘要：
AIM: To observe the effect of acupuncture at Foot-Yangming Meridian on gastric mucosal blood flow (GMBF), gastric motility and brain-gut peptide. METHODS: Sixty SD rats were randomly divided into 6 groups: normal control group, model group (group with gastric mucosal damage, GMD), Sibai group (with acupuncture at Sibai point + GMD), Tianshu group (with acupuncture at Tianshu point + GMD), Zusanli group (with acupuncture at Zusanli point + GMD) and non-acupoint group (with acupuncture at non-acupoint + GMD). The GMD model group was induced by infusing pure alcohol into gastric cavity. H-2 Gas Clearance Test (HGCT) was used to measure GMBF, the frequency and amplitude of gastric motility were measured by the method of aerocyst, the content of brain-gut peptide in sinus ventriculi and bulbus medullae were detected by radioimmunoassay. RESULTS: Inhibitory effect of the frequency and amplitude of gastric motility were shown in model group, and the rates of frequency and amplitude changes were remarkably different from the normal control group (-19.41 +/- 17.21 vs -4.71 +/- 10.32, P < 0.05; -51.61 +/- 29.02 vs 1.81 +/- 14.12, P < 0.01). In comparison with control group, the GMBF was 0.52 +/- 0.161 mL vs 1.03 +/- 0.255 mL per 100g tissue/min, P < 0.01, the content of motilin in sinus ventriculi and bulbus medullae was 63.04 +/- 7.77 pg/mL vs 72.91 +/- 8.42 pg/mL, P < 0.05 and 50.96 +/- 8.77 pg/mL vs 60.76 +/- 8.05 pg/mL, P < 0.05, but the content of somatostatin in sinus ventriculi and bulbus medullae was 179.85 +/- 43.13 ng/g vs 90.54 +/- 40.42 ng/g, P < 0.01 and 532.86 +/- 122.58 ng/g vs 370.91 +/- 76.29 ng/g, P < 0.05, respectively. In comparison with model group, the amplitude of gastric motility was 1.52 +/- 20.13, -6.52 +/- 23.31, 6.92 +/- 25.21 vs -51.61 +/- 29.02, P < 0.01 and GMBF was 0.694 +/- 0.160 mL vs 0.893 +/- 0.210 mL, 1.038 +/- 0.301 mL vs 0.52 +/- 0.161 mL per 100g tissue/min, P < 0.01, respectively in Tianshu, Sibai and Zusanli groups, the content of motilin in sinus ventriculi and bulbus medullae was 71.64 +/- 9.35 pg/mL vs 63.04 +/- 7.77 pg/mL, P < 0.05; 58.96 +/- 6.69 pg/mL vs 50.96 +/- 8.77 pg/mL, P < 0.05 in Zusanli group and 72.84 +/- 8.62 pg/mL vs 63.04 +/- 7.77 pg/mL, P < 0.05 in Sibai group, while the content of somatostatin in bulbus medullae in Tianshu, Sibai and Zusanli groups was 480.62 +/- 151.69 ng/g, 388.53 +/- 155.04 ng/g, 365.76 +/- 143.93 ng/g vs 532.86 +/- 122.58 ng/g, P < 0.05, respectively. CONCLUSION: Electro-acupuncture at acupoints of Foot-Yangming Meridian could enhance the gastric motility, improve GMBF, and regulate the content of motilin and somatostatin in sinus ventriculi and bulbus. The effects of acupuncture on GMBF and gastric motility may be related to the content of brain-gut peptide. (c) 2007 The WJG Press. All rights reserved.
摘要：
Three new diastereomeric shikimic acid analogues (4-amino-3,5-dihydroxyl-cyclohex-1-en-carboxylic acids, 16) bearing a C-4 amino group were synthesized in parallel by a mixture-operation protocol. Ring-closing enyne metathesis (RCEYM) under ethylene atmosphere was successfully employed to construct the desired carbocycles in high efficiency. The absolute configurations of each final product were confirmed by the ID and 2D NMR techniques. (C) 2007 Elsevier Ltd. All rights reserved.
期刊：
Journal of Ethnopharmacology,2007年113(2):292-299 ISSN：0378-8741
通讯作者：
Cai, Guangxian
作者机构：
[Cai, Guangxian] Hunan Tradit Chinese Med Univ, Changsha 410007, Peoples R China.;Chinese Univ Hong Kong, Sch Chinese Med, Hong Kong, Hong Kong, Peoples R China.;Univ Hong Kong, Sch Chinese Med, Hong Kong, Hong Kong, Peoples R China.;Univ Hong Kong, Mol Chinese Med Lab, Hong Kong, Hong Kong, Peoples R China.;So Med Univ, Sch Chinese Med, Guangzhou 515015, Peoples R China.
通讯机构：
[Cai, Guangxian] H;Hunan Tradit Chinese Med Univ, Changsha 410007, Peoples R China.
摘要：
Buyang Huanwu Decoction is a classic formula for treating stroke-induced disability in traditional Chinese medicine (TCM). To explore its pharmacological basis, we investigated the effects of the whole formula and its herbal components on the neurological behavior performance and infarction volume in focal cerebral ischaemia rats. The neurological deficit scores and infarction volume were measured at days 3, 7 and 14 after 30 min of occlusion of middle cerebral artery. The results showed that Buyang Huanwu Decoction and its herbal components significantly improved the neurological behavior performances and reduced the infarction volume in the ischaemic brains. To elucidate the potential therapeutic mechanisms, we investigated the proliferation of progenitors by detecting the immunohistochemical staining of thymidine analog 5-bromo-2'-deoxyuridine (BrdU) and found that the formula stimulated the proliferation of the progenitors at hippocampus and subventricular zone (SVZ) in the ischaemic brains. As vascular endothelial growth factor (VEGF) and its receptor fetal liver kinase (Flk1) are important neurotrophic, neuroprotective and neuroproliferative factors, we studied the expressions of VEGF and Flk1 in the hippocampus, SVZ and cortex in the ischaemic brains and found that the formula led to increase the numbers of VEGF-positive and Flk1-positive cells in the SVZ and cortex in the ischaemic brains. The results indicate that the therapeutic effects of Buyang Huanwu Decoction for recovery of neurological deficits are associated with the stimulation of the proliferation of progenitors and the enhancement of the expressions of VEGF and Flk in ischaemic brains. (C) 2007 Elsevier Ireland Ltd. All rights reserved.
作者机构：
[Zhou, Hai-Bin; Gao, Zheng-Xi; Zhong, Chun-Long; Chen, Lei; Zhang, Yan-Hong; Yao, Zhu-Jun; Wu, Wen-Min] Chinese Acad Sci, Shanghai Inst Organ Chem, State Key Lab Bioorgan & Nat Prod Chem, Shanghai 200032, Peoples R China.;[Peng, Xin-Jun; Zhang, Yan-Hong] Hunan Tradit Chinese Med Univ, Dept Anal Chem, Changsha 410007, Hunan, Peoples R China.;[Zhong, Chun-Long; Yao, Zhu-Jun] Univ Sci & Technol China, Dept Chem, Anhua 230026, Peoples R China.;[Yao, Zhu-Jun] Chinese Acad Sci, Shanghai Inst Organ Chem, State Key Lab Bioorgan & Nat Prod Chem, 345 Fenglin Rd, Shanghai 200032, Peoples R China.
通讯机构：
[Yao, Zhu-Jun] C;Chinese Acad Sci, Shanghai Inst Organ Chem, State Key Lab Bioorgan & Nat Prod Chem, 345 Fenglin Rd, Shanghai 200032, Peoples R China.
摘要：
Labeling of bioactive small molecules with organic dyes for various applications in cell biology has been emerging as an attractive research field. Using an easily prepared and inexpensive fluorescein derivative 1 and a Cu(I)-catalyzed Huisgen reaction, an efficient fluorescent labeling strategy is developed generally for bioactive natural products. Essentials of a successful labeling include the personalized introduction of an azido functionality to specific targets by a selective and efficient manner, and the strategic adjustment of reaction sequence to avoid possible side reactions under the 'click' reaction conditions. Such a protocol has been successfully applied to the fluorescent labeling of four bioactive small molecules in different chemical categories in this study. Advantages of this labeling protocol include the use of inexpensive reagents, ease of operation, free-of-protections at the 'click' step, and suiting a wide range of bioactive molecules bearing the reactive functionalities. (c) 2007 Elsevier Ltd. All rights reserved.
摘要：
AIM: To observe the effects of pre-moxibustion on apoptosis and proliferation of gastric mucosal cell in rats with stress-induced ulcer, and to analyze the relationship between those effects and the expression of heat-shock protein 70 (HSP70). METHODS: Sixty healthy Sprague Dawley rats were randomly assigned into four groups, namely group A, B, C and D. The animal model of stress ulcer was established by water immersion and restraint stress. The rats in group A, 13, and D served as the restraint, model, and non-acupoint controls, respectively, while those in group C received moxibustion at Zusanli and Liangmen points. Immunohistochemical methodology was used to detect the expression of HSP70, apoptosis index (AI, x 10(-6)/mu m(2)) and proliferation index (PCNA-LI, x 10(-6)/mu m(2)). The mucosal expression of transforming growth factor a (TGF-alpha) was detected by radioimmunoassay. RESULTS: Moxibustion at Zusanli and Liangmen points significantly decreased the gastric injury and the apoptosis of gastric mucosal cells, while markedly increased the mucosal expression of TGF-alpha and HSP70 as well as the proliferation of gastric mucosal cells. Compared with group A, ulcer index (UI) (26.8 +/- 9.8 vs 12.0 +/- 5.9, P < 0.01), AI (9.6 +/- 4.2 vs 4.4 +/- 2.6, P < 0.05) and expression of HSP70 (9.6 +/- 4.2 vs 4.4 +/- 2.6, P < 0.05) were significantly increased, but the content of TGF-alpha (104.7 +/- 51.2 pg/mL vs 254.0 +/- 86.9 pg/mL, P < 0.01) and PCNA-LI (6.9 +/- 4.7 vs 14.9 +/- 4.6, P < 0.05) were significantly decreased in group B. However, ulcer index values (W) and AI were obviously lower in group C compared to groups B and D (14.1 +/- 5.4 vs 26.8 +/- 9.8 and 26.2 +/- 7.7, P < 0.01; 3.0 +/- 1.6 vs 9.6 +/- 4.2 and 8.2 +/- 5.2, P < 0.05, respectively), but content of TGF-alpha (237.0 +/- 72.6 pg/mL vs 104.7 +/- 51.2 pg/mL and 154.1 +/- 61.3 pg/mL, P < 0.01) and expression of HSP70 (0.13 +/- 0.03 vs 0.08 +/- 0.06 and 0.06 +/- 0.04, P < 0.05) were higher in group C. Furthermore, the PCNA-LI was significantly higher in group C than in group B (21.6 +/- 4.1 vs 6.9 +/- 4.7, P < 0.01). CONCLUSION: Moxibustion at Zusanli and Liangmen points has a protective effect on rats gastric mucosa in stress-induced gastric ulcer, which is closely related to its actions in promoting synthesis of TGF-alpha and proliferation of gastric mucosal cells, suppressing gastric mucosal cell apoptosis, and up-regulating HSP70 expression. (c) 2007 The WJG Press. All rights reserved.
摘要：
AIM: To explore the mechanism of the exciting effects of electro-acupuncture (EA) at Sibai on the gastric myoelectric activities. METHODS: A total of 32 rats were randomly divided into four groups. Through intraperitoneal injection with atropine (the anti-cholinergic agent by blockade of muscarinic receptors), hexamethonium (automatic nerve ganglion-blocking agent) and reserpine (anti-adrenergic agent by depleting the adrenergic nerve terminal of its norepinephrine store), effects of EA at Sibai on the gastric myoelectric activities of the denervated rats were observed. RESULTS: After intraperitoneal injection of atropine and hexamethonium, the average amplitude and ratio of period to time in the phase of high activity of gastric myoelectric slow wave, and the average numbers of the peaks of gastric myoelectric fast wave were significantly decreased (P < 0.01, P < 0.05, P < 0.01), while after intraperitoneal injection of reserpine, the aforementioned three parameters were increased (P < 0.01, P < 0.05, P < 0.01). EA at Sibai point partially relieved the inhibitory effect of atropine and hexamethonium on the gastric myoelectric activities in the rats (P < 0.05 or P > 0.05). CONCLUSION: Cholinergic and adrenergic nervous systems and autonomic nerve ganglion participate in the peripheral passage of the controlling effects of EA at Foot Yangming Channel on gastrointestinal tract. (C) 2006 The WJG Press. All rights reserved.
作者机构：
[Ai Huang; Shou-Xiang Yi; Xi-Ping Li; Ya-Ping Lin; Jie Yan; Xiao-Rong Chang; Zong-Bao Yang; Yan, J; Rong Hu] Hunan Univ Tradit Chinese Med, Inst Acupuncture & Moxibust, Changsha 410007, Peoples R China.
通讯机构：
[Jie Yan] H;Hunan Univ Tradit Chinese Med, Inst Acupuncture & Moxibust, Changsha 410007, Peoples R China.
关键词：
EA;relative particularity between Stomach Meridian of Foot-Yangming;gastric mucosal damage;stress;intestinal trefoil factor;gene expression
摘要：
AIM: To investigate electroacupunture(EA) at the acupoints of Stomach Meridian of Foot-Yangming(SMFY), Gallbladder Meridian of Foot-Yangming(SMFY) on gastric mucosal intestinal trefoil factor (ITF) gene expression detection in stress-induced rats with gastric mucosal lesion, and to explore the regulatory mechanism and significance of EA-related gastric mucosal protective effect. METHODS: Forty rats were randomly divided into 4 groups: Blank group, Model group, Model group+EA at acupoints of SMFY group("SMFY group's, and Model group+EA at acupoints of GMFY group(GMFY group). All rats (except blank group) were made model by water immersion and restraint stress (WRS). Then the gastric mucosa tissue in each rat was taken off after assessment of gastric mucosal lesion index(GUI), and the expression of ITF mRNA of the tissues was detected by reverse transcription-polymerase chain reaction(RT-PCR) method. RESULTS: Compared with Model group(54.3 +/- 1.34), the GUI value in SMFY group (31 +/- 2.21) decreased significantly(P < 0.01), so did that in GMFY group (39.8 +/- 1.62, P < 0.05), meanwhile GUI value in SMFY group was significantly lower than in GMFY group(P < 0.01). Compared with Model group (0.65 +/- 0.01), EA had a tendency to improve the expression of gastric mucosal ITFmRNA gene: such tendency existed in GMFY group (0.66 +/- 0.01) but with no signficant difference(P > 0.05), in SMFY group(0.76 +/- 0.01) with an extremely obvious difference (P < 0.01), furthermore the expression in SMFY group was significantly higher than in GMFY group (P < 0.01). CONCLUSION: The gastric mucosal protective effect by EA at the acupoints of SMFY and GMFY was related to the expression variance of ITF, indicating certain meridian specificity exists. It could be one proof for the TCM theory "Relative particularity between SMFY and stomach". (C) 2006 The WJG Press. All rights reserved.
通讯机构：
[Yan, Jie] H;Hunan Univ Tradit Chinese Med, Coll Acupuncture & Moxibust, Changsha 410007, Hunan Province, Peoples R China.
关键词：
electroacupuncture;serum;stomach meridian acupoints;gastric mucosal cells;epidermal growth Factor receptor;gene expression
摘要：
AIM: To investigate the effect of serum derived from rats treated with electroacupuncture at stomach meridian acupoints on the expression of epidermal growth factor receptor (EGFR) gene in gastric mucosal cells. METHODS: The stress-induced gastric mucosal injury in rat model was established by water-immersion and restrained stress methods. 52 rats were randomly divided into: normal group (n = 8), model group (n = 8), model serum group (n = 12), stomach serum group (n = 12), and gallbladder serum group (n = 12). The gastric mucosal cells were separated by pronase-EDTA digestion method and incubated with serum. The EGFR gene expression in gastric mucosal cells was detected by reverse transcription-polymerase chain reaction (RT-PCR) method. RESULTS: Compared with normal group (0.6860 +/- 0.0594), the serum derived from rats of the stomach group (1.2272 +/- 0.0813, P = 0.00 < 0.01) and gallbladder group (0.9640 +/- 0.0387, P = 0.00 < 0.01) had a tendency to enhance the EGFR gene expression in gastric mucosal cells. Such tendency existed in the model group (0.7104 +/- 0.0457) but with no significant difference (P = 0.495 > 0.05) and in model serum group (0.8516 +/- 0.0409) with an extremely obvious difference (P = 0.001 < 0.01). Furthermore, the EGFR gene expression in stomach serum group was significantly higher than that in gallbladder serum group (P = 0.00 < 0.01). CONCLUSION: The present study shows that serum derived from rats treated with electroacupuncture at stomach meridian acupoints can distinctly increase the EGFR gene expression of gastric mucosal cells. Therefore, there is certain meridian specificity in the serum, which could provide a proof for the TCM theory "particular relation between meridian and internal organ". (C) 2006 The WJG Press. All rights reserved.
摘要：
AIM: To discuss the protective effect of electroacupuncture at the Foot-Yangming Meridian on gastric mucosal lesion, somatostatin (SS) and the expression of SS receptor genes (SSRimRNA) in rabbits with gastric ulcer and to further explore the relative specificity of meridians and viscera at gene expression level. METHODS: Forty rabbits were randomly divided into control group (A), gastric ulcer model group (B), Foot-Yangming Meridian group (C), Foot-Shaoyang Meridian group (D) and Foot-Taiyang Meridian group (E). The gastric ulcer model was prepared by infusing alcohol into stomach. Groups C-E were treated with electroacupuncture at points along the above meridians using meridian stimulating instruments for 7 days respectively. By the end of treatment, the index of gastric ulcer was determined, the amount of epidermal growth factor(EGF) and somatostatin was measured by radioimmunoassay (RIA). SS-R(1)mRNA expression in gastric mucosa was determined by RT-PCR. RESULTS: The value of EGF in model group was obviously lower(73.6 +/- 14.8 vs 91.3 +/- 14.9 pg/mL, P < 0.01) than that in control group. The index of gastric ulcer, content of SS and expression of SSR(1)mRNA in gastric mucosa were significantly higher than those in control group (24.88 +/- 6.29 vs 8.50 +/- 2.98 scores, P < 0.01; 2978.6 +/- 587.6 vs 1852.4 +/- 361.7 mIU/mL, P < 0.01; 2.56 +/- 0.25 vs 1.04 +/- 0.36, P < 0.01). The value of EGF in Foot-Yangming Meridian group was higher than that in model group(92.2 +/- 6.7 vs 73.6 +/- 14.8 pg/mL, P < 0.01). The index of gastric ulcer, content of SS and expression of SS-R1mRNA in gastric mucosa were significantly lower than those in control group(10.88 +/- 3.23 vs 24.88 +/- 6.29 scores, P < 0.01; 1800.2 +/- 488 vs 2978.6 +/- 587.6 mIU/mL, P < 0.01; 1.07 +/- 0.08 vs 2.56 +/- 0.25 mIU/mL, P < 0.01). Compared to the model group, the content of SS and expression of SSR(1)mRNA in gastric mucosa in Foot-Shaoyang Meridian group decreased (2441.0 +/- 488. vs 2978.6 587.6 mIU/mL, P < 0.05;1.73 +/- 0.16 vs 2.56 0.25 mIU/mL, P < 0.01). But the above parameters in Foot-Taiyang Meridian group did not improve and were significantly different from those in Foot-Yangming Meridian group (P < 0.05) CONCLUSION: Electro-acupuncture at Foot-Yangming Meridian can protect gastric mocusa against injury. The mechanism may be releted to the regulation of brain-gut peptides and the expression of SSRimRNA. (c) 2006 The WIG Press. All rights reserved.
通讯机构：
[Yan, Jie] H;Hunan Coll Tradit Chinese Med, Inst Acupuncture & Moxibust, Physiol Lab, Changsha 410007, Peoples R China.
关键词：
NTS;Firing Neuron;Gastric Distention;Meridian and Viscera;Acupuncture
摘要：
The nucleus of the solitary tract (NTS) is a primary center, not only for receiving visceral afferents, but also for somatic afferents. Previous experiments demonstrated that NTS might be a primary afferent center for connecting the Stomach Meridian (ST Medirian) and the Stomach. In this study, an extracellular recording method was used to analyze the firing neurons. The variety of NTS' neurons' responses to gastric distention (GD) and different body surface points were compared. In 52 rats, 104 firing neurons of NTS were recorded, in which there were 47 GD related neurons. For the 47 GD related neurons, there were 33 neurons responsive to acupuncture at Sibai (ST 2, belonging to ST Meridian). Therefore, in the 47 GD related neurons, the response neuron occurring rate (RNOR) for acupuncture at ST 2 was 70.21%. Meanwhile in the 47 GD related neurons, when acupuncture was performed at Quanliao (SI 18, belonging to Small Intestine Meridian) and non-acupoint respectively, there were 18 and 17 firing neurons, and the RNOR were 38.30% and 36.17% respectively. The experiment demonstrated that there were some varieties in the response of NTS neurons to GD stimuli and acupuncture at different body surface points, which suggested that the ST Meridian was more intimately linked to the Stomach and that the different acupoints do have some specificity.
