作者机构：
[侯昕玥; 王健全] Eye Hospital, China Academy of Chinese Medical Sciences, Beijing 100040, China;[侯昕玥] China Academy of Chinese Medical Sciences, Beijing 100040;[亢泽峰] Eye Hospital, China Academy of Chinese Medical Sciences, Beijing 100040, China. zefeng2531@163.com;[杨迎新] Beijing Hospital of TCM, Capital Medical University;[姚小萍] Shenzhen Hospital of TCM
摘要：
Seco-triterpenoids are a unique class of triterpenoids possessing distinct structural features. Based on the differences in their parent nucleus, they can be divided into two categories: tetracyclic triterpenes and pentacyclic triterpenes, which are widely distributed across various plant species. Previous studies indicate that natural seco-triterpenoids have diverse chemical structures and exhibit extensive biological activities, including anti-tumor, anti-inflammatory, hypoglycemic, and neuroprotective effects. This review comprehensively summarizes recent research (2020–2023) on seco-triterpenoids and derived saponins, encompassing their chemical structures, plant distributions, pharmacological activities, structure–activity relationships, and future development trends. Furthermore, we utilized the latest bibliometric tool, VOSviewer, to conduct a keyword cluster analysis, enabling us to identify current research hotspots and patterns. Besides, we have cataloged and analyzed 351 compounds, inclusive of 298 tetracyclic triterpenoids and 53 pentacyclic triterpenoids, in order to provide valuable references for activity mining and structural modification of seco-triterpenoids, facilitating further clinical applications and developments. Collectively, seco-triterpenoids represent an important class of natural products with significant potential for pharmacological use. By summarizing recent research achievements, this review provides a beneficial resource for scientists in the field, promoting the exploration and development of seco-triterpenoid-based drugs.
期刊：
CURRENT NEUROPHARMACOLOGY,2024年22(10):1672-1696 ISSN：1570-159X
作者机构：
Key Laboratory of Hunan Province for Integrated Traditional Chinese and Western Medicine on Prevention and
Treatment of Cardio-Cerebral Diseases, College of Integrated Traditional Chinese Medicine and Western Medicine,
Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;Third-Grade
Pharmacological Laboratory on Chinese Medicine Approved by State Administration of Traditional Chinese Medicine,
College of Medicine and Health Sciences, China Three Gorges University, Yichang, Hubei 443002, China;Department of Neurosurgery, First Affiliated
Hospital of Hunan University of Traditional Chinese Medicine, Changsha, Hunan 410007, China;School
of Pharmacy, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;[Gong, Lipeng; Liang, Junjie; Xie, Letian] Key Laboratory of Hunan Province for Integrated Traditional Chinese and Western Medicine on Prevention and
Treatment of Cardio-Cerebral Diseases, College of Integrated Traditional Chinese Medicine and Western Medicine,
Hunan University of Chinese Medicine, Changsha, Hunan 410208, China
摘要：
Ischemic stroke is a leading cause of disability and death worldwide. However, the clinical efficacy of recanalization therapy as a preferred option is significantly hindered by reperfusion injury. The transformation between different phenotypes of gliocytes is closely associated with cerebral ischemia/ reperfusion injury (CI/RI). Moreover, gliocyte polarization induces metabolic reprogramming, which refers to the shift in gliocyte phenotype and the overall transformation of the metabolic network to compensate for energy demand and building block requirements during CI/RI caused by hypoxia, energy deficiency, and oxidative stress. Within microglia, the pro-inflammatory phenotype exhibits upregulated glycolysis, pentose phosphate pathway, fatty acid synthesis, and glutamine synthesis, whereas the anti-inflammatory phenotype demonstrates enhanced mitochondrial oxidative phosphorylation and fatty acid oxidation. Reactive astrocytes display increased glycolysis but impaired glycogenolysis and reduced glutamate uptake after CI/RI. There is mounting evidence suggesting that manipulation of energy metabolism homeostasis can induce microglial cells and astrocytes to switch from neurotoxic to neuroprotective phenotypes. A comprehensive understanding of underlying mechanisms and manipulation strategies targeting metabolic pathways could potentially enable gliocytes to be reprogrammed toward beneficial functions while opening new therapeutic avenues for CI/RI treatment. This review provides an overview of current insights into metabolic reprogramming mechanisms in microglia and astrocytes within the pathophysiological context of CI/RI, along with potential pharmacological targets. Herein, we emphasize the potential of metabolic reprogramming of gliocytes as a therapeutic target for CI/RI and aim to offer a novel perspective in the treatment of CI/RI.
期刊：
ITALIAN JOURNAL OF PEDIATRICS,2024年50(1):1-12 ISSN：1720-8424
通讯作者：
Li-hui Zhu
作者机构：
[Hua-yan Liu; Yue-wei Chen; Shan Zeng; Jian-hui Xie] Hunan Children's Hospital, Changsha, Hunan, China;[Xiao-yan Tan; Min Yi; Xia Wu] Hunan University of Chinese Medicine, Changsha, Hunan, China;[Li-hui Zhu] Hunan Children's Hospital, Changsha, Hunan, China. ylw0520@163.com
通讯机构：
[Li-hui Zhu] H;Hunan Children’s Hospital, Changsha, China
关键词：
Epilepsy;Family management;Children;Simulation training
摘要：
BACKGROUND: Epilepsy is a chronic neurological disorder that is more likely to be diagnosed in children. The main treatment involves long-term use of anti-epileptic drugs and above all, home care is of great importance. As there has not been a widely accepted home care protocols, simulating a home care environment is necessary for caregivers to develop skills of proper home care. This study aims to evaluate the effectiveness of a simulation training of family management style (STOFMS) for parents of children with epilepsy in China. METHODS: A randomized controlled trial was conducted on 463 children with epilepsy and their families. They were recruited from March 2020 to November 2022 and randomly assigned to the STOFMS group or the conventional group in a 1:1 ratio. Scores of family management measures, 8-item of Morisky Medication Adherence and epilepsy clinical symptom of both groups were collected at three points of time: within 24h after admission (T0), 3months after discharge (T1), and 6months after discharge (T2). Changes due to intervention were compared across groups by repeated-measures ANOVA. The study report followed the CONSORT 2010 checklist. RESULTS: There were statistically significant differences between the two groups at T2. A considerable increase over the baseline was observed in the total management level score and subscale scores in the STOFMS group at T1, compared with essentially no change in the control group. In terms of medication adherence, the STOFMS group performance improved greatly at T1 and T2 compared with the control group. The same result was also found in clinical efficacy at T2 (p < 0.05). CONCLUSION: STOFMS is an effective intervention to improve family management level, treatment adherence and clinical efficacy for children with epilepsy. TRIAL REGISTRATION: The registration number is ChiCTR2200065128. Registered at 18 October 2022, http://www.medresman.org.cn.
关键词：
Chinese herbal compound;Large intestine cancer;AOM/DSS-Induced colorectal cancer mice;16S rRNA;Inflammation
摘要：
This study was conducted to observe the effect of Chinese herbal compound on the treatment of colon cancer using AOM/DSS-induced C57BL/6J colon cancer mice and to validate potential influence on intestinal flora of mice. A colorectal cancer (CRC) mouse model was built with a total of 50 C57BL/6J mice that were induced by administrating AOM/DSS. These experimental animals were split up into 5 groups, a control group, a model group, and low-, medium- and high-dose Chinese herbal compound groups. All mice were given Chinese herbal compound treatment, and the colon tissues of each group were harvested with the length measured and the number of colon polyps accounted. The Ki-67 expression in the colon tissues was detected via immuno-histochemistry. Relative quantification of the expression of genes and proteins was determined through qPCR and WB assays. Contents of IL-6, TNF-α, IFN-γ, and IL-10 in serum and colon tissues of mice were determined by ELISA. An additional 16S rRNA sequencing analysis was implemented for the identification of mouse intestinal flora. The results suggested that all low-, medium- or high-dose Chinese herbal compound could markedly inhibit the shortening of colon length and significant number reduction of colon polyps in the model group. The relative expression of genes and proteins (PCNA, Muc16, and MMP-9) associated with proliferation in mouse colon tissues were inhibited. In addition, compared with the model group, the contents of IL-6, TNF-α, and IFN-γ in serum and colon tissues were substantially decreased in the high-dose Chinese herbal compound group, thereby reducing the structure damage in colon tissues and the infiltration degree of inflammatory cells. Besides, the expression of TLR4/MyD88/NF-κB protein was markedly decreased. The 16S rRNA sequencing analysis demonstrated that mice in the model group had decreased intestinal flora diversity, and there were significant changes in flora abundance and amino acid metabolism between the control group and the model group. Taken together, the treatment of Chinese herbal compound against CRC in this study might be regulated by the TLR4/MyD88/NF-κB signaling pathway, and the imbalance in intestinal flora was also closely related to CRC occurrence.
期刊：
TISSUE ENGINEERING AND REGENERATIVE MEDICINE,2024年21(1):185-197 ISSN：1738-2696
通讯作者：
Zhang, Ying;Cao, XY
作者机构：
[Zhang, Leilei; Cao, XY; Zhang, Ying; Cao, Xiangyang] Luoyang Orthoped Traumatol Hosp, Orthoped Hosp Henan Prov, Med Ctr Hip, 82 Qiming South Rd, Luoyang 471002, Henan, Peoples R China.;[Li, Chuan] 920Th Hosp Joint Logist Support Force, Dept Oncol, Kunming 650032, Yunnan, Peoples R China.;[Wei, Qiushi; He, Wei] Guangzhou Univ Tradit Chinese Med, Inst Orthopaed, Guangzhou 510240, Peoples R China.;[Wei, Qiushi; He, Wei] Guangzhou Univ Tradit Chinese Med, Affiliated Hosp 3, Guangzhou 510240, Peoples R China.;[Jing, Zhenhao; Yuan, Qiang; Dong, Yiping] Henan Univ Tradit Chinese Med, Zhengzhou 450046, Henan, Peoples R China.
通讯机构：
[Cao, XY ; Zhang, Y] L;Luoyang Orthoped Traumatol Hosp, Orthoped Hosp Henan Prov, Med Ctr Hip, 82 Qiming South Rd, Luoyang 471002, Henan, Peoples R China.
关键词：
Osteonecrosis of the femoral head;Sustained release system;PEG-PLGA-PLL nanoparticle;MiR-320a;Bone regeneration
摘要：
BACKGROUND: This study aimed to explore the effect of a nanomaterial-based miR-320a inhibitor sustained release system in trauma-induced osteonecrosis of the femoral head (TIONFH). METHODS: The miR-320a inhibitor-loaded polyethylene glycol (PEG)- Poly(lactic-co-glycolic acid) (PLGA)- Poly-L-lysine (PLL) nanoparticles were constructed using the double emulsion method. The TIONFH rabbit model was established to observe the effects of miR-320a inhibitor nanoparticles in vivo. Hematoxylin-eosin staining and microcomputed tomography scanning were used for bone morphology analysis. Bone marrow mesenchymal stem cells (BMSCs), derived from TIONFH rabbits, were used for in vitro experiments. Cell viability was determined using the MTT assay. RESULTS: High expression of miR-320a inhibited the osteogenic differentiation capacity of BMSCs in vitro by inhibiting the expression of the osteoblastic differentiation markers ALP and RUNX2. MiR-320a inhibitor-loaded PEG-PLGA-PLL nanoparticles were constructed with a mean loading efficiency of 1.414 ± 0.160%, and a mean encapsulation efficiency of 93.45 ± 1.24%, which released 50% of the loaded miR-320a inhibitor at day 12 and 80% on day 18. Then, inhibitor release entered the plateau. After treatment with the miR-320a inhibitor nanoparticle, the empty lacunae were decreased in the femoral head tissue of TIONFH rabbits, and the osteoblast surface/bone surface (Ob.S/BS), osteoblast number/bone perimeter (Ob.N/B.Pm), bone volume fraction, and bone mineral density increased. Additionally, the expression of osteogenic markers RUNX2 and ALP was significantly elevated in the TIONFH rabbit model. CONCLUSION: The miR-320a inhibitor-loaded PEG-PLGA-PLL nanoparticle sustained drug release system significantly contributed to bone regeneration in the TIONFH rabbit model, which might be a promising strategy for the treatment of TIONFH.
作者机构：
[Zheng, Yu; Zheng, Huizhen] Hunan Univ Chinese Med, Clin Coll Chinese Med 1, Changsha 410007, Peoples R China.;[Zheng, Yu; Zheng, Huizhen; Guo, Zhihua] Hunan Univ Chinese Med, Coll Chinese Med, Changsha 410208, Peoples R China.
通讯机构：
[Guo, ZH ] H;Hunan Univ Chinese Med, Coll Chinese Med, Changsha 410208, Peoples R China.
关键词：
Bayesian model;Clinical efficacy;Heart failure with (mildly) reduced ejection fraction;Network meta-analysis;Traditional Chinese medicine injection
摘要：
BACKGROUND: More than half of all heart failure (HF) patients have heart failure with reduced ejection fraction (HFrEF) or mildly reduced ejection fraction (HFmrEF). The combination of traditional Chinese medicine injections (TCMIs) with Western medicine treatment (WMT) has been reported to have better efficacy than using WMT alone. However, the positive effects of TCMIs combined with WMT on HFrEF and HFmrEF require more comprehensive and systematic evidence and warrant further investigation. METHODS: The NMA searched eight databases, including four English and four Chinese, from database creation to November 10, 2022. We used the Cochrane Risk of Bias tool (ROB 2) to assess the selected studies' quality. OpenBUGS and STATA 17.0 were used for network meta-analysis. RESULTS: The 101 RCTs were included in the systematic review. Studies have shown that when combined with any of the five TCMIs, WMT was more efficient than WMT alone. Shenmai injection (SMI)+WMT may be the best treatment for clinical effectiveness rate (CER) improvement and b-type natriuretic peptide (BNP) reduction. Huangqi injection (HQI)+WMT was the best treatment for improving left ventricular ejection fraction (LVEF). Danhong injection (DHI)+WMT may be the best treatment for lowering left ventricular end-diastolic dimension (LVEDD). Xinmailong injection (XMLI)+WMT was likely the best treatment for increasing the 6-min walking test (6MWT). In addition, XMLI had the lowest incidence of adverse reactions (3.38%). CONCLUSIONS: Shenfu injection (SFI), SMI, DHI, XMLI, and HQI combined with WMT have stronger efficiency in treating HFrEF and HFmrEF. However, as all studies were conducted in China, this review is limited by the inevitable selection bias, and further high-quality multicenter randomized controlled trials (RCTs) are required.
摘要：
目的:利用网络药理学方法和实验验证分析脊痛消胶囊治疗腰椎间盘突出症(lumbar disc herniation,LDH)的作用机制.方法:从中药系统药理学(Traditional Chinese Medicine Systems Pharmacology,TCMSP)、中药分子机理的生物信息学分析工具(Bioinformatics Analysis Tool for Molecular Mechanism of Traditional Chinese Medicine,BATMAN-TCM)、中医药资料@Taiwan和Swiss数据库中检索并筛选脊痛消胶囊的有效成分及其作用靶点,并使用GeneCards、人类孟德尔遗传(Online Mendelian Inher-itance in Man,OMIM)和DisGeNET数据库预测LDH的疾病靶点,再将活性成分作用靶点与疾病靶点进行映射,得到脊痛消胶囊治疗LDH潜在靶点,继续在STRING数据库中进行蛋白互作分析(protein-protein interaction,PPI),将结果导入Cytoscape软件获取PPI网络图和"药物-活性成分-潜在靶点"网络图.利用clusterProfiler包对潜在靶点进行基因本体(Gene Ontology,GO)功能、京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)通路富集分析,用Autodock Vina和Discovery Studio软件对活性成分和关键靶点进行分子对接验证.最后以脊痛消胶囊为干预因素,在LDH模型大鼠进行实验验证.结果:网络药理学结果显示,共收集到脊痛消胶囊活性成分139 种、靶点479 个,获得LDH靶点590 个,映射得到84 个交集靶点,通过PPI网络筛选得到脊痛消胶囊治疗LDH的关键治疗靶点 10 个:信号转导和转录激活因子 3(signal transducer and activator of transcription 3,STAT3)、转录因子 JUN(transcription factor Jun-1,JUN)、白细胞介素(interleukin,IL)-6、IL-10、丝裂原活化蛋白激酶 1(mitogen-activated protein kinase 1,MAPK1)、FOS蛋白(protein c-fos,FOS)、连环素(catenin beta-1,CTNNB1)、丝裂原活化蛋白激酶14(MAPK14)、蛋白激酶B(protein kinase B,PKB/AKT1)和肿瘤坏死因子(tumor nec-rosis factor,TNF),GO功能富集分析显示交集靶点涉及2 163 种生物学过程、36 种细胞组分和102 种分子功能,KEGG通路富集分析显示,共涉及155 条信号通路,分子对接结果显示核心靶点与成分对接结合良好.实验验证结果显示,脊痛消胶囊可显著降低LDH模型大鼠血清IL-6 和TNF-α水平,提高血清IL-10 水平,降低髓核组织p-JUN/JUN、p-FOS/FOS、p-JNK/JNK和p-p38MAPK/p38MAPK水平.结论:脊痛消胶囊可以通过抑制炎症反应和JNK/MAPK信号通路的激活从而起到保护LDH模型大鼠的作用.
期刊：
CURRENT MOLECULAR MEDICINE,2024年 ISSN：1566-5240
作者机构：
[Xiong, Wu] Department of Burns and Plastic Surgery, the First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, 410007, China;[Zhang, Xi] Clinical Medical School of Hunan University of Chinese Medicine, Hunan Brain Hospital, Changsha, 410007, China;[Zou, Xiao-Ling] Department of Endocrinology, the First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, 410007, China;[Peng, Sai; Lei, Hua-Juan] Department of Anesthesiology, the First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, 410007, China;[Liu, Xiang-Nan] College of Acupuncture & Tuina and Rehabilitation, Hunan University of Chinese Medicine, Changsha, 410208, China
摘要：
BACKGROUND: Chronic hyperglycemia in diabetes induces oxidative stress, leading to damage to the vascular system. In this study, we aimed to evaluate the effects and mechanisms of AS-IV-Exos in alleviating endothelial oxidative stress and dysfunction caused by high glucose (HG). METHODS: Histopathological changes were observed using HE staining, and CD31 expression was assessed through immunohistochemistry (IHC). Cell proliferation was evaluated through CCK8 and EDU assays. The levels of ROS, SOD, and GSH-Px in the skin tissues of each group were measured using ELISA. Cell adhesion, migration, and tube formation abilities were assessed using adhesion, Transwell, and tube formation experiments. ROS levels in HUVEC cells were measured using flow cytometry. The levels of miR-210 and Nox2 were determined through quantitative reverse transcription-polymerase chain reaction (qRT-PCR). The expression of Nox2, SOD, GSH-Px, CD63, and CD81 was confirmed using WB. RESULTS: The level of miR-210 was reduced in diabetes-induced skin damage, while the levels of Nox2 and ROS increased. Treatment with AS-IV increased the level of miR-210 in EPC-Exos. Compared to Exos, AS-IV-Exos significantly reduced the proliferation rate, adhesion number, migration speed, and tube-forming ability of HGdamaged HUVEC cells. AS-IV-Exos also significantly decreased the levels of SOD and GSH-Px in HG-treated HUVEC cells and reduced the levels of Nox2 and GSH-Px. However, ROS levels and Nox2 could reverse this effect. CONCLUSION: AS-IV-Exos effectively alleviated endothelial oxidative stress and dysfunction induced by HG through the miR-210/Nox2/ROS pathway.
