作者机构：
[Tang, Zhenwang] S China Univ, Affiliated Hosp 1, Dept Cardiol, Hengyang 421001, Peoples R China.;[Li, Xingsheng] Chongqing Med Univ, Affiliated Hosp 2, Dept Geriatr Cardiol, Chongqing 400010, Peoples R China.;[Liao, Duanfang] Hunan Univ Chinese Med, Dept Pharmacol, Changsha 410007, Hunan, Peoples R China.
通讯机构：
[Tang, Zhenwang] S;S China Univ, Affiliated Hosp 1, Dept Cardiol, Hengyang 421001, Peoples R China.
关键词：
The beta 2-adrenergic receptor gene;Polymorphism;Hypertension;Meta-analysis
摘要：
The beta 2-adrenergic receptor (beta 2-AR) gene has been implicated in the pathogenesis of hypertension. However, the results have been conflicting. In this study, we performed a meta-analysis to assess the associations of 46A/G and 79C/G polymorphisms in beta 2-AR gene with hypertension risk in Han Chinese population. Published literature from PubMed, ISI Web of Science, Embase databases, CNKI, CBM and Wanfang Data were retrieved. Pooled odds ratio (OR) with 95 % confidence interval (CI) was calculated using fixed- or random-effects model. Eleven studies (2,058 cases and 1,459 controls) for 46A/G polymorphism and eight studies (2,219 cases and 1,495 controls) for 79C/G polymorphism were identified. The results suggested that 46A/G polymorphism G allele might increase the risk of hypertension (GG vs. AA: OR = 1.47, 95 % CI 1.20-1.82). However, no significant association was observed for 79C/G polymorphism (GG vs. CC: OR = 1.05, 95 % CI 0.61-1.79). The results indicated that 46A/G polymorphism in the beta 2-AR gene was associated with hypertension susceptibility in Han Chinese population.
摘要：
Objectives: To study the effects of group reminiscence therapy on depressive symptoms, self-esteem, and affect balance among community dwelling elderly. Methods: Eight communities were randomly selected from 372 eligible communities in Changsha city. They were randomly divided into four experimental groups and four control group. Geriatric Depression Scale (GDS) was used to screen entire 478 older adults living in these 8 communities. One hundred and sixty five of them had a GDS score between 11 and 25; among them, 125 participated in the study finally. The elderly group who were in the control group received health education, while the intervention group received both health education and group reminiscence therapy for 6 weeks. Both groups were assessed with the GDS, Self-Esteem Scale (SES), and Affect Balance Scale (ABS) before and after the 6-week-intervention. The results were analyzed using a mixed effect model with fixed effect of the intervention and random effect of the community, incorporating the structured randomness at the community level. Results: After 6 weeks' therapy, the GDS scores in the intervention group decreased significantly compared to those in the control group (p < 0.001). Scores on the positive affect subscale and affect balance in the intervention group increased significantly higher than control group, and scores on the negative affect subscale decreased significantly lower than control group (p < 0.01). After the intervention, there were no statistically significant differences in self-esteem scores between intervention and control groups. Conclusions: Group reminiscence therapy was effective in reducing symptoms of depression, improving affect balance, and promoting mental health of community-dwelling elderly. (C) 2011 Elsevier Ireland Ltd. All rights reserved.
摘要：
Essential oils from the fruit of two species of Evodia rutaecarpa from China (Evodia rutaecarpa (Juss.) Benth. var. officinalis (Dode) Huang and Evodia rutaecarpa (Juss.) Benth.) have been obtained by hydrodistillation and analysed by gas chromatography-mass spectrometry in order to discern the differences and similarities between the volatile chemical compositions of these species. More than 21 components were identified in essential oils of the studied plants. In the oil of E. rutaecarpa (Juss.) Benth. var. officinalis (Dode) Huang, the main essential oil ingredients were beta-myrcene (44.43%) and beta-pinene (39.88%). beta-pinene (72.82%), 1R-alpha-pinene (8.90%) and beta-myrcene (1.99%) were the major compounds in the oil of E. rutaecarpa (Juss.) Benth. The chemical compounds of the essential oils showed that there are only six common compounds between the two species.
摘要：
One of our previous studies showed that Yizhijiannao Granule,a compound Chinese medicine, effectively improved the clinical symptoms of Alzheimer’s disease.In the present study,we established a model of Alzheimer’s disease using beta-amyloid(25-35)in PC12 cells,and treated the cells with Yizhijiannao Granule and its four monomers,i.e.,icariin,catechin,Panax notoginseng saponins,and eleutheroside E.Flow cytometry showed that Yizhijiannao Granule-containing serum, icariin,Panax notoginseng saponins,and icariin+Panax notoginseng saponins were protective against beta-amyloid(25-35)-induced injury in PC12 cells.Icariin in combination with Panax notoginseng saponins significantly inhibited early apoptosis of PC12 cells with beta-amyloid (25-35)-induced injury compared to icariin or Panax notoginseng saponins alone.The effects of icariin+Panax notoginseng saponins were similar to the effects of Yizhijiannao Granule.The findings indicate that two of the effective monomers of Yizhijiannao Granule,icariin and Panax notoginseng saponins,can synergistically inhibit early apoptosis of PC12 cells induced by beta-amyloid(25-35).
通讯机构：
[Chang, Xiaorong] H;Hunan Univ Chinese Med, Coll Acupuncture & Tuina, Changsha 410007, Hunan, Peoples R China.
关键词：
pre-moxibustion;transection of neural pathway;Zusanli (ST36);gastric mucosal lesion;heat shock proteins-70;ulcer index;traditional Chinese medicine;neural regeneration
摘要：
Studies have shown that pre-moxibustion protects the gastric mucosa by up-regulating the expression of heat shock protein 70. However, the signaling pathway underlying this effect remains unclear. Rats were intragastrically administered absolute alcohol, causing obvious lesion of the gastric mucosa. Following pre-moxibustion at Zusanli (ST36) for 8 days, the ulcer index decreased to different degrees. The results of an enzyme linked immunosorbent assay and western blotting showed significant upregulation of heat shock protein 70 expression in the gastric mucosa and serum. None out of transection of the spinal cord, damage to the nucleus of the solitary tract, neurotomy of the vagal nerve and neurotomy of the common peroneal nerve affected the decrease in ulcer index or the increase in heat shock protein 70 expression in serum after pre-moxibustion at Zusanli, and heat shock protein 70 expression was obviously decreased in the gastric mucosa. These findings suggest that pre-moxibustion at Zusanli can protect the gastric mucosa against lesioning, and that the mechanism underlying this effect involves its induction of heat shock protein 70 expression. Neural pathways participate in the regulatory effects of moxibustion on heat shock protein 70 expression in the gastric mucosa.
期刊：
Chemical Physics Letters,2012年527:73-78 ISSN：0009-2614
通讯作者：
Huang, Ying
作者机构：
[Huang, Ying; Liu, Lianghong] Hunan Univ Chinese Med, Sch Pharm, Changsha 410208, Hunan, Peoples R China.;[Liu, Shubin] Univ N Carolina, Ctr Res Comp, Chapel Hill, NC 27599 USA.
通讯机构：
[Huang, Ying] H;Hunan Univ Chinese Med, Sch Pharm, Changsha 410208, Hunan, Peoples R China.
摘要：
In this study, a rat model of inflammatory pain was produced by injecting complete Freund’s adjuvant into the hind paw, and the expression of acetylated histone 3 in the spinal cord dorsal horn was examined using immunohistochemical staining. One day following injection, there was a dramatic decrease in acetylated histone 3 expression in spinal cord dorsal horn neurons. However, on day 7, expression recovered in adjuvant-injected rats. While acetylated histone 3 labeling was present in dorsal horn neurons, it was more abundant in astrocytes and microglial cells. The recovery of acetylated histone 3 expression was associated with a shift in expression of the protein from neurons to glial cells. Morphine injection significantly upregulated the expression of acetylated histone 3 in spinal cord dorsal horn neurons and glial cells 1 day after injection, especially in astrocytes, preventing the transient downregulation. Our results indicate that inflammatory pain induces a transient downregulation of acetylated histone 3 in the spinal cord dorsal horn at an early stage following adjuvant injection, and that this effect can be reversed by morphine. Thus, the downregulation of acetylated histone 3 may be involved in the development of inflammatory pain.
作者机构：
[Wei Xing; Wang Dong-sheng; Wan Ling; Li Ji-yang; Zhong Guang-wei] Cent S Univ, Xiangya Hosp, Changsha 410008, Hunan, Peoples R China.;[Wang Zhan-yi] Henan Yuzhou Chinese Med Hosp, Yuzhou 461670, Peoples R China.;[Duan Xiao-peng; He Fu-yuan] Hunan Tradit Chinese Med Univ, Changsha 410208, Hunan, Peoples R China.;[Jiang Yao] Cent S Univ, State Key Lab Powder Met, Changsha 410083, Peoples R China.
通讯机构：
[Wang Dong-sheng] C;Cent S Univ, Xiangya Hosp, Changsha 410008, Hunan, Peoples R China.
关键词：
Ti-Al intermetallic compound porous materials;Dahuangzhecong Fang;arterial thrombosis;isolated Chinese medicine
摘要：
The mechanism of antithrombotic of Dahuangzhechong Fang separated and purified by Ti-Al intermetallic compound porous material (TAICPM) was researched. Dahuangzhechong Fang, which was isolated and screened by TAICPM, was used to oral rats. At the end of study, their blood and thrombus were collected. The results show that TAICPM with the pore size of 1-5 μm can screen Dahuangzhechong Fang well. Dahuangzhechong Fang can increase 6-keto-PGF1α, lower content of TXD2 and platelet. Dahuangzhechong Fang has good effect to resist arterial thrombosis.
作者机构：
[Zhang, He-Ming; Deng, Gui-Ming] S China Normal Univ, Coll Biophoton, MOE Key Lab Laser Life Sci, Guangzhou 510631, Guangdong, Peoples R China.;[Zhang, He-Ming; Deng, Gui-Ming] S China Normal Univ, Coll Biophoton, Inst Laser Life Sci, Guangzhou 510631, Guangdong, Peoples R China.;[Wang, Chao-Run; Chen, Zhen; Deng, Gui-Ming] Hunan Univ Chinese Med, Affiliated Hosp 1, Changsha 410007, Hunan, Peoples R China.
通讯机构：
[Zhang, He-Ming] S;S China Normal Univ, Coll Biophoton, MOE Key Lab Laser Life Sci, Guangzhou 510631, Guangdong, Peoples R China.
摘要：
In the title molecule, C 16H 14N 2O, the dihedral angle between the two phenyl rings is 23.5(6)°. In the crystal, N-H-O hydrogen bonds link molecules into chains running along the a axis.
摘要：
Rats with chronic pilocarpine-induced temporal lobe epilepsy complicated with depression were studied. Anti-5-bromodeoxyuridine immunofluorescence staining and Timms staining showed that neurogenesis within the hippocampal dentate gyrus and mossy fiber sprouting were increased in model rats. Neurogenesis within the hippocampal dentate gyrus was further enhanced, while mossy fiber sprouting was decreased in model rats administered carbamazepine alone or in combination with the 5-hydroxytryptamine 1A receptor agonist, 8-hydroxy-dipropylaminotetralin (0.1 and 1 mg/kg). Among the groups, the effect was the most significant in rats receiving carbamazepine in conjunction with 1 mg/kg 8-hydroxy-dipropylaminotetralin. Thus, high dose 8-hydroxy-dipropylaminotetralin can improve neural plasticity in epileptic rats with depression.
期刊：
Journal of Thermal Analysis and Calorimetry,2012年109(1):395-402 ISSN：1388-6150
通讯作者：
Xiao, Xiaohe
作者机构：
[Dai, Bing; Li, Xingfeng] Hunan Univ Tradit Chinese Med, Coll Pharm, Changsha 410208, Hunan, Peoples R China.;[Zhou, Jian; Yan, Dan; Wang, Jiabo; Xiao, Xiaohe; Zhao, Yanling; He, Jing; Jin, Cheng] 302 Mil Hosp China, China Mil Inst Chinese Mat Med, Beijing 100039, Peoples R China.;[Wang, Haitao] 306 Mil Hosp, Dept Pharm, Beijing 100101, Peoples R China.;[Xiao, Xiaohe] 302 Mil Hosp China, China Mil Inst Chinese Mat Med, 100 W 4th Ring Middle Rd, Beijing 100039, Peoples R China.
通讯机构：
[Xiao, Xiaohe] 3;302 Mil Hosp China, China Mil Inst Chinese Mat Med, 100 W 4th Ring Middle Rd, Beijing 100039, Peoples R China.
作者机构：
[Chen, Yinzhuang; Xu, Guangming; Yao, Shouzhuo] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410081, Hunan, Peoples R China.;[Chen, Bo; Wang, Hua] Hunan Normal Univ, Key Lab Chem Biol & Tradit Chinese Med Res, Minist Educ China, Changsha 410081, Hunan, Peoples R China.;[Xu, Guangming] Hunan Univ Chinese Med, Coll Pharmaceut Sci, Changsha 410208, Hunan, Peoples R China.;[Ding, Li] Shimadzu Res Lab Shanghai Co Ltd, Shanghai, Peoples R China.
通讯机构：
[Chen, Bo] H;Hunan Normal Univ, Key Lab Chem Biol & Tradit Chinese Med Res, Minist Educ China, Changsha 410081, Hunan, Peoples R China.
摘要：
Desorption corona beam ionization mass spectrometry, a novel direct MS analysis technique, was first employed to probe intermediates in Leuckart reaction between ketone and ammonium formate at 185 °C to corroborate the reaction mechanism.
摘要：
A middle cerebral artery occlusion-model was established in rats using the improved thread embolism method.Rats were treated with acupuncture at either Dazhui(DU14),Renzhong(DU26), Baihui(DU20),or a non-meridian point.Detection with protein-chip technology showed that the level of protein phosphorylation in both groups was upregulated or downregulated depending on the signaling pathway compared with the model group that did not receive acupuncture.Analysis of proteins showing downregulated phosphorylation revealed that five signaling pathways were activated in the acupuncture-treatment group,while only two were activated in the acupuncture-control group.In contrast,analysis of proteins showing upregulated phosphorylation revealed only one pathway was activated in the acupuncture-treatment group,whereas four pathways were activated in the acupuncture-control group.Furthermore,the number of activated proteins in the acupuncture-treatment group was not only higher than the acupuncture-control group,but unlike the acupuncture-control group,the majority of activated proteins were key proteins in the signaling pathways.Our findings indicate that acupuncture at specific points can activate multiple signaling pathways to promote the restoration of brain tissue following ischemic injury,and that this is based on a combination of effects resulting from multiple pathways,targets,and means.
摘要：
AIM: To explore the effect of Qingguangan on the expressions of MMP-2 and MMP-9 in filtering bleb scarring area after trabeculectomy in rabbit model. METHODS: Thirty-two New Zealand rabbits were randomized into four groups: control group, experimental group, MMC group (ocular trabeculectomy in combination with MMC), and Qingguangan group. Trabeculectomy was performed on both eyes in each group except control group. Qingguangan group was mouth-fed with Qingguangan (solution). On postoperative day 14, the appearances of MMP-2 and MMP-9 on filtrating blebs were observed by immunohistochemistry. RESULTS: Statistical differences of the expressions of MMP-2 and MMP-9 were noted among groups on day 14 following surgery. Histology immunohistochemistry showed significant differences on the expressions of MMP-2 and MMP-9 between each groups( P<0.05). CONCLUSION: Qingguangan can promote the expressions of MMP-2 and MMP-9.
摘要：
CONTEXT: Chinese patent medicine Si-Mo-Tang oral liquid preparation (SMT) is composed of Aucklandia luppa Decne (Compositae), Citrus aurantium Linn (Rutaceae), Lindera aggregata (Sims) Kosterm (Lauraceae), and Areca catechu Linn (Arecaceae). Studies of SMT have been impeded due to the lack of quality control methods. OBJECTIVE: This study aimed to simultaneously determine three alkaloids including synephrine, arecoline, and norisoboldine in SMT for the first time. MATERIALS AND METHODS: A strong cation exchange (SCX) high performance liquid chromatography (HPLC) method was developed to simultaneously determine synephrine, arecoline, and norisoboldine in SMT, and was compared with ion-pairing chromatography using regular reversed-phase chromatography columns. System suitability parameters of synephrine, arecoline, and norisoboldine using the SCX chromatography column were investigated. RESULTS: Results demonstrated that good separations were achieved on an Agilent SCX (250 x 4.6 mm, 5 microm) column at 35 degrees C. The mobile phase consisting of methanol-0.2% phosphoric acid was delivered at a constant flow of 1.0 mL min(-1) and the eluent was monitored at 215 nm. The HPLC method showed good linearity for the examined concentration ranges of 2.55-255.0, 1.30-208.0, and 2.06-201.6 microg mL(-1) for synephrine, arecoline, and norisoboldine, respectively. The limits of quantification (S/N = 10) were 2.55, 1.30, and 2.06 microg mL(-1), the limits of detection (S/N = 3) were 1.53, 0.78, and 1.21 microg mL(-1), and average recoveries were 98.99, 95.63 and 99.04%, respectively, for synephrine, arecoline, and norisoboldine. DISCUSSION AND CONCLUSION: This method has been successfully applied to determine synephrine, arecoline, and norisoboldine in Chinese patent medicine SMT.
