作者机构：
[Li, Xiaoya; Qiao, Bo; Tan, Zhoujin; Yu, Rong; Deng, Na] Hunan Univ Chinese Med, Sch Tradit Chinese Med, Changsha 410208, Peoples R China.;[Peng, Xinxin] Hunan Univ Chinese Med, Affiliated Hosp 1, Dept Pediat, Changsha 410208, Peoples R China.;[Peng, Maijiao] Hunan Univ Chinese Med, Sch Pharm, Changsha 410208, Peoples R China.
通讯机构：
[Rong Yu; Zhoujin Tan] S;School of Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha 410208, China
关键词：
SCFAs;adenine combined with Folium sennae-induced diarrhea;characteristic bacteria;gut microbiota;intestinal inflammatory response;kidney function
摘要：
Abstract: Background: Extensive evidence suggests that gut microbiota may interact with the kidneys and play central roles in the pathogenesis of disease. However, the association of gut microbiota-kidneys in diarrhea remains unclear. Methods: A diarrhea mouse model was constructed by combining adenine with Folium sennae. We analyzed the characteristics of the gut content microbiota and short chain fatty acids (SCFAs); and explored the potential link between gut content microbiota, SCFAs, intestinal inflammatory response and kidney function. Results: Characteristic bacteria Lactobacillus intestinalis and Bacteroides acidifaciens were enriched in the gut contents of mice. The productions of SCFAs were remarkably inhibited. Model mice presented an increased trend of creatinine (Cr), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α), a decreased trend of blood urea nitrogen (BUN) and secretory immunoglobulin A (SIgA). The pathological analysis proved obvious damage to the kidney structure. Lactobacillus intestinalis and Bacteroides acidifaciens exisited in the correlations with acetic acid, intestinal inflammatory response and kidney function. Conclusions: Adenine combined with Folium sennae-induced diarrhea, altered the structure and function of the gut content microbiota in mice, causing the enrichment of the characteristic bacteria Lactobacillus intestinalis and Bacteroides acidifaciens. The interactions between Lactobacillus intestinalis, Bacteroides acidifaciens and acetic acid, intestinal inflammation, and kidney function might be involved in the process of gut-kidney impairment in adenine, combined with Folium sennae-induced diarrhea. Keywords: gut microbiota; adenine combined with Folium sennae-induced diarrhea; characteristic bacteria; SCFAs; intestinal inflammatory response; kidney function
期刊：
Tropical Journal of Pharmaceutical Research,2022年21(9):1939-1949 ISSN：1596-5996
作者机构：
[Wang, Dan; Han, Haicheng; Yang, Yong] Hangzhou Normal Univ, Sch Publ Hlth, Hangzhou, Peoples R China.;[Fang, Rui] Hunan Univ Chinese Med, Sch Integrated Chinese & Western Med, Changsha, Peoples R China.;[Fu, Xiaoqing; Yang, Yong] Zhejiang Chinese Med Univ, Hangzhou TCM Hosp, Hangzhou, Peoples R China.;[Rui, Kangle] Hangzhou Gongshu Hosp Integrated Tradit & Western, Hangzhou, Peoples R China.
关键词：
COVID-19;Depression;Wei-Sheng-Fang-Yi-Bao-Dan;Network pharmacology;Molecular;docking;Traditional Chinese medicine (TCM)
摘要：
Purpose: To investigate the mechanisms of action of Wei-Sheng-Fang-Yi-Bao-Dan (WSFYBD) in the treatment of COVID-19 and depression using network pharmacology and molecular docking. Methods: First, the bioactive components and target genes of WSFYBD were retrieved from TCMSP database. The relevant gene targets of depression and COVID-19 were obtained from databases. The core WSFYBD genes for treatment were separately obtained by determining gene intersection. Cytoscape 3.8.0 software was used to draw the visual interactive networks. STRING database was employed to construct protein-protein interaction networks, while Gene Ontology (GO), and Kyoto Encyclopedia of Genes and Genomes (KEGG) functional enrichment analyses were used to determine the function and pathway of target genes via a Bioconductor/R. Finally, AutoDockTools software was employed for molecular docking. Results: A total of 105 potential bio-active components and 35 target genes of WSFYBD for COVID-19 therapy were identified. Also, 1905 GO entries (p < 0.05) and 158 related signal pathways (p < 0.05) for COVID-19 were obtained. Similarly, 114 potential bio-active components of WSFYBD and 127 potential therapeutic targets of depression were identified. Moreover, 1948 GO entries (p < 0.05) and 177 related signal pathways for depression were retrieved (p < 0.05). Docking results showed the main bio-active Conclusion: The mechanisms for treating COVID-19 show that WSFYBD directly acts on SARS-CoV-2 virus to prevent it from entering the host cell, or inhibits virus replication. Secondly, WSFYBD ameliorates depression by acting on key targets that control over-activated cytokines. Therefore, WSFYBD has potentials for the management of COVID-19 and depression.
摘要：
To study the effect of berberine combined with formononetin on the migration of NPC cells, and to preliminarily explore the roles of the MAPK/ERK1/2 signaling pathway.Berberine combined with formononetin synergistically inhibited the proliferation, migration and invasion of NPC cells. After treatment, the expression of EMT-related proteins N-cadherin,vimentin and MAP4K4 decreased, that of E-cadherin increased, and that of p-c-Raf, p-MEK, p-ERK1/2 was down-regulated. After MAPK/ERK 1/2 signaling pathway was activated by EGF, the inhibitory effect of berberine combined with formononetin on the key proteins p-c-Raf, p-MEK, p-ERK1/2 of MAPK/ERK1/2 signaling pathway, and the downstream proteins N-cadherin and vimentin, and the promotion of E-cadherin were weakened. Meanwhile, the activation of MAPK/ERK1/2 signaling pathway reduced the inhibitory effect of berberine combined with formononetin on the migration of NPC cells.Berberine combined with formononetin decreases the activity of the MAPK/ERK1/2 signaling pathway, thus further inhibiting the migration of NPC cells.
作者：
Nie, Zhi;Pu, Tong;Han, Zhaojie;Wang, Chenyang;Pan, Chenglong;...
期刊：
Frontiers in Oncology,2022年12:2732 ISSN：2234-943X
通讯作者：
Jiang, X.
作者机构：
[Nie, Zhi] Kunming Med Univ, Affiliated Hosp 1, Dept Neurol, Kunming, Peoples R China.;[Nie, Zhi] Yunnan Prov Clin Res Ctr Neurol Dis, Dept Neurol, Kunming, Peoples R China.;[Nie, Zhi; Jiang, Xiulin] Chinese Acad Sci & Yunnan Prov, Kunming Inst Zool, Key Lab Anim Models & Human Dis Mech, Kunming, Peoples R China.;[Pu, Tong] Hunan Univ Chinese Med, Coll Acupuncture & Tuina & Rehabil, Changsha, Peoples R China.;[Han, Zhaojie] Army Med Univ, Southwest Hosp, Dept Thorac Surg, Chongqing, Peoples R China.
通讯机构：
Key Laboratory of Animal Models and Human, Disease Mechanisms of Chinese Academy of Sciences and Yunnan Province, Kunming Institute of Zoology, Kunming, China
关键词：
Extra spindle pole bodies-like 1;Lung Adenocarcinoma;Prognosis biomarker;DNA Methylation;Immune infiltration
摘要：
Extra spindle pole bodies-like 1 (ESPL1), a cysteine endopeptidase, plays a vital role in chromosome inheritance. However, the association of ESPL1 with prognosis and immune infiltration in lung adenocarcinoma (LUAD) has not yet been explored. Here, we analyzed the expression level, prognostic values, diagnostic value, and immune infiltration level in LUAD using various databases. Immunohistochemistry (IHC) and quantitative real-time PCR (qRT-PCR) assays were used to detect the expression of ESPL1 in LUAD tissues and cell lines. In this study, we found that ESPL1 was upregulated in LUAD and a higher expression of ESPL1 was correlated with unfavorable prognosis in LUAD. Meanwhile, Cox hazard regression analysis results suggested that ESPL1 may be an independent prognostic factor for LUAD. Moreover, we demonstrated that ESPL1 expression was significantly correlated with immune infiltration of Th2 and dendritic cells in LUAD. We also confirmed that DNA copy number amplification and DNA hypo-methylation were positively correlated with ESPL1 expression in LUAD. Additionally, DNA copy number amplification was significantly associated with adverse clinical outcomes in LUAD. Kyoto Encyclopedia of Genes and Genomes (KEGG) and gene set enrichment analysis (GSEA) confirmed that ESPL1 was mainly involved in the DNA replication and glycolysis signaling pathway. Finally, we revealed that ESPL1 was highly expressed in LUAD tissues and cell lines. Knockdown of ESPL1 significantly inhibited cell migration and the invasion abilities of LUAD. Our study comprehensively confirmed that ESPL1 expression may serve as a novel prognostic biomarker for both the clinical outcome and immune cell infiltration in LUAD.
摘要：
Gemcitabine (GEM) is one of the first-line chemotherapies for bladder cancer (BC), but the GEMs cannot recognize cancer cells and have a low long-term response rate and high recurrence rate with side effects during the treatment of BC. Targeted transport of GEMs to mediate cytotoxicity to tumor and avoid the systemic side effects remains a challenge in the treatment of BC. Based on a firstly confirmed biomarker in BC-protein tyrosine kinase 7 (PTK7), which is overexpressed on the cell membrane surface in BC cells, a novel targeting system protein tyrosine kinase 7 aptamer-Gemcitabine conjugate (PTK7-GEMs) was designed and synthesized using a specific PTK7 aptamer and GEM through auto-synthesis method to deliver GEM against BC. In addition, the antitumor effects and safety evaluation of PTK7-GEMs was assessed with a series of in vitro and in vivo assays. PTK7-GEMs can specifically bind and enter to BC cells dependent on the expression levels of PTK7 and via the macropinocytosis pathway, which induced cytotoxicity after GEM cleavage from PTK7-GEMs respond to the intracellular phosphatase. Moreover, PTK7-GEMs showed stronger anti-tumor efficacy and excellent biosafety in three types of tumor xenograft mice models. These results demonstrated that PTK7-GEMs is a successful targeted aptamer-drug conjugates strategy (APDCs) to treat BC, which will provide new directions for the precision treatment of BC in the field of biomarker-oriented tumor targeted therapy.
期刊：
Frontiers in Pharmacology,2022年13:1105804 ISSN：1663-9812
通讯作者：
Sun, K.-W.
作者机构：
[Sun, Ke-Wei; Tang, Dan; Wu, Yunan; Ding, Lin; Wang, Ruo-Yu] Hunan Univ Tradit Chinese Med, Dept Hepatol, Hosp 1, Changsha, Peoples R China.;[Mo, Yang] Hunan Univ Tradit Chinese Med, Acad Affairs Off, Changsha, Peoples R China.
通讯机构：
[Sun, K.-W.] D;Department of Hepatology, China
关键词：
Acute-on-chronic liver failure (ACLF);Wenyang Jiedu Huayu (WYJDHY) formula;Network Pharmacology;Atractylenolide I (AT-1);rat hepatocytes;ACLF rat model
摘要：
BACKGROUND: Colorectal adenoma (CRA) is the main cause of the progression of Colorectal adenocarcinoma (COAD). Therefore, it is very important to accurately reveal its developmental mechanism. METHODS: Differential expression genes (DEGs) in three microarray datasets were screened using GEO and GEO2R. R packages were used for gene ontology (GO) functional annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) path enrichment analysis. Hub genes screened by STRING, Cytoscape and CytoHubba were used. R was used for DEGs of hub genes, and Gene Expression Profiling Interactive Analysis (GEPIA2) database was used for prognostic Analysis. R-packet were used to analyze tumor pathology, tumour, lymph-nodes, and metastases (TNM) staging, enrichment, immune invasion and prognosis. RESULTS: Among the 66 genes, including 36 up-regulated and 30 down-regulated genes. Survival analysis showed that COL1A1, COL5A2, COL5A1 and secreted protein acidic and rich in cysteine (SPARC) were associated with disease-free survival in patients. The four genes were related to tumor pathological stage, TNM stage and immune invasion. COL1A1 and COL5A2 were highly expressed in chromatin modification and cellular senescence. Low expression of COL5A1 and SPARC was significantly enriched in neutrophil degranulation and Wp VegfavegFR2 signaling pathways. CONCLUSIONS: Obviously, these four key genes can serve as important targets for early diagnosis, treatment, immunity and prognosis of CRA to COAD.
摘要：
BACKGROUND Drain-site hernia（DSH） has an extremely low morbidity and has rarely been reported. Small bowel obstruction is a frequent concurrent condition in most cases of DSH, which commonly occurs at the ≥ 10 mm drain-site. Here we report a rare case of DSH at the lateral 5 mm port site one month postoperatively without visceral incarceration. Simultaneously, a brief review of the literature was conducted focusing on the risk factors, diagnosis, and prevention strategies for DSH.CASE SUMMARY A 76-year-old male patient was admitted to our institution with intermittent abdominal pain and a local abdominal mass which occurred one month after laparoscopic radical resection of rectal cancer one year ago. A computed tomography scan showed an abdominal wall hernia at the 5 mm former drain-site in the left lower quadrant, and that the content consisted of the large omentum. An elective herniorrhaphy was performed by closing the fascial defect and reinforcing the abdominal wall with a synthetic mesh simultaneously. The postoperative period was uneventful. The patient was discharged seven days after the operation without surgery-related complications at the 1-mo follow-up visit.CONCLUSION Emphasis should be placed on DSH despite the decreased use of intra-abdominal drainage. It is recommended that placement of a surgical drainage tube at the ≥ 10 mm trocar site should be avoided. Moreover, it is advisable to have a comprehensive understanding of the risk factors for DSH and complete closure of the fascial defect at the drainage site for high-risk patients.
期刊：
Journal of Ethnopharmacology,2022年294:115363 ISSN：0378-8741
通讯作者：
Xuyi Tan
作者机构：
[Tan, Xuyi; Shi, Qiyun; Duan, Jiahao] Hunan Univ TCM, Changsha 410208, Hunan, Peoples R China.;[Shi, Qiyun] Luoyang Orthoped Traumatol Hosp Henan Prov, Orthoped Hosp Henan Prov, Luoyang 471000, Henan, Peoples R China.;[Huang, Lu; Kuang, Gaoyan; Lu, Min] Affiliated First Hosp Hunan Univ Tradit Chinese Me, Changsha 410007, Hunan, Peoples R China.;[Tan, Xuyi] Affiliated Hosp Hunan Acad Chinese Med Sci, Dept Orthoped Surg, Changsha 410006, Hunan, Peoples R China.
通讯机构：
[Xuyi Tan] H;Hunan University of TCM, Changsha, Hunan, 410208, China<&wdkj&>Department of Orthopedic Surgery, Affiliated Hospital of Hunan Academy of Chinese Medical Science, Changsha, Hunan, 410006, China
摘要：
ETHNOPHARMACOLOGICAL RELEVANCE: Knee osteoarthritis (KOA) is one of the common age-degenerative diseases. Recent studies have demonstrated that the pathogenesis of KOA is closely related to synovial lesions. Jiawei Duhuo Jisheng mixture (JDJM) has shown great potential in the treatment of KOA. However, the effect and mechanism of JDJM on synovial lesions of KOA remain unclear. AIM OF THE STUDY: The regulatory effect of JDJM on the Wnt/β-catenin signaling pathway in KOA inflamed synovium was studied via in vitro and in vivo experiments, respectively. MATERIALS AND METHODS: For the in vitro experiment, fibroblasts were isolated from the rabbit synovium with KOA. The fibroblasts were grouped as follows: the vehicle group was given 0.5% FBS; the inhibitor group was treated with 0.5% volume fraction of XAV939; the normal serum groups and JDJM serum groups were treated with 5%, 10%, and 20% volume fractions of normal serum and JDJM-containing serum. The expression levels of Wnt3a, β-catenin, Cyclin D1, metalloproteinase-7(MMP-7) and cyclooxygenase-2(COX-2) were detected by different assays 48 and 72h after the intervention. For the in vivo experiment, the rabbit KOA model was prepared using the improved Hulth modeling method, whereby all rabbits were randomly divided into normal control, model control, positive control, and traditional Chinese medicine (TCM) groups. The expression levels of Wnt3a, β-catenin, Cyclin D1, MMP-7 and COX-2 were detected by different assays in the 2, 4, and 8 weeks of treatment. RESULTS: In the two test results of in vitro experiments, the normal serum group was compared with the JDJM-containing serum group with the same volume fraction, demonstrating that mRNA transcription and protein expression levels of Wnt3a, β-catenin, Cyclin D1, MMP-7, and COX-2 in the latter decreased (P<0.05), with more pronounced effects observed in the group treated with 20% volume fraction of JDJM serum. Compared with the inhibitor group, there was no significant difference (P>0.05) in the mRNA transcription and protein expression levels, i.e., Wnt3a, β-catenin, Cyclin D1, and MMP-7 were observed in the JDJM serum groups, except for a significant decrease (P<0.05) in the level of mRNA transcription and protein expression of COX-2. Based on the in vivo experiment, compared to the model control group, articular cartilage, synovial hyperplasia, and the inflammatory reaction of the TCM group at different treatment times were significantly improved. The mRNA transcription level of Wnt3a, β-catenin, Cyclin D1, MMP-7 and COX-2 detected by RT-qPCR and the protein expression level of Wnt3a, β-catenin, Cyclin D1, MMP-7 and COX-2 detected by Western blot were significantly reduced (P<0.05), and the effect was more evident at the eighth week. CONCLUSION: JDJM can regulate the synovial Wnt/β-catenin signaling pathway in KOA models, reduce the mRNA transcription and protein expression levels of Wnt3a, β-catenin, Cyclin D1, MMP-7, and COX-2 in the synovium, thus inhibiting synovial inflammation and protecting articular cartilage, which could be the key mechanism of action in treating this disease.
作者机构：
[Xie, Qing-ling; Li, Bin; Wang, Wei; Peng, Cai-yun; Yang, Yu-pei; Yu, Huang-he; Liu, Yong-bei; Jian, Yu-qing; Wang, Bin] Hunan Univ Chinese Med, Innovat Mat Med Res Inst, TCM & Ethnomed Innovat & Amp Dev Int Lab, Sch Pharm, Changsha 410208, Hunan, Peoples R China.
通讯机构：
[Wei Wang] T;TCM and Ethnomedicine Innovation & Development International Laboratory, Innovative Materia Medica Research Institute, School of Pharmacy, Hunan University of Chinese Medicine, Changsha, People's Republic of China
摘要：
Heilaohu, the roots of Kadsura coccinea, has been used in Tujia ethnomedicine to treat rheumatic arthritis (RA). Heilaohuacid G (1), a new 3,4-seco-lanostane type triterpenoid isolated from the ethanol extract of Heilaohu, whose structure was determined using HR-ESI-MS data, NMR spectroscopic analyses, and ECD calculations. In this study, our purpose is to elucidate the mechanisms of Heilaohuacid G in the treatment of RA by inhibited proliferation of rheumatoid arthritis-fibroblastoid synovial (RA-FLS) cells and inhibited the inflammatory reactions in LPS-induced RA-FLS and RAW 264.7 cell lines via inhibiting NF-κB pathway. The biological activity screening experiments indicated that Heilaohuacid G significantly inhibited proliferation of RA-FLS cells with IC(50) value of 8.16 ± 0.47μM. CCK-8 assay, ELISA, flow cytometry assay, and Western blot were used to measure the changes of cell viability, apoptosis, and the release of inflammatory cytokines. Heilaohuacid G was found not only induced RA-FLS cell apoptosis, but also inhibited the inflammatory reactions in LPS-induced RA-FLS and RAW 264.7 cell lines via inhibiting NF-κB pathway. Furthermore, Heilaohuacid G (p.o.) at doses of 3.0, 6.0, and 12.0 mg/kg and the ethanol extracts of Heilaohu (p.o.) at doses of 200, 400, and 800 mg/kg both were confirmed antiinflammatory effects on xylene-induced ear mice edema model.
作者机构：
[Luo, Lu; Gong, Limin; Wang, Wei; Qiu, Yixing; Wen, Ruiding] Hunan Univ Chinese Med, Sch Pharm, Innovat Mat Med Res Inst, TCM & Ethnomed Innovat & Dev Int Lab, Changsha 410208, Peoples R China.;[Gong, Limin] Macau Univ Sci & Technol, Sch Chinese Med, Macau 999078, Peoples R China.
通讯机构：
[Limin Gong; Wei Wang] A;Authors to whom correspondence should be addressed.<&wdkj&>TCM and Ethnomedicine Innovation and Development International Laboratory, Innovative Materia Medic Research Institute, School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China<&wdkj&>Authors to whom correspondence should be addressed.<&wdkj&>TCM and Ethnomedicine Innovation and Development International Laboratory, Innovative Materia Medic Research Institute, School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China<&wdkj&>School of Chinese Medicine, Macau University of Science and Technology, Macau 999078, China
关键词：
genus Polygonatum;classification;chemical composition;processing;pharmacological effect
摘要：
Ethnopharmacological relevance: The genus Polygonatum Tourn, ex Mill. contains numerous chemical components, such as steroidal saponins, polysaccharides, flavonoids, alkaloids, and others, it possesses diverse pharmacological activities, such as anti-aging, anti-tumor, immunological regulation, as well as blood glucose management and fat reducing properties. Aim of the review: This study reviews the current state of research on the systematic categorization, chemical composition, pharmacological effects, and processing changes of the plants belonging to the genus Polygonatum, to provide a theoretical foundation for their scientific development and rational application. Materials and methods: The information was obtained by searching the scientific literature published between 1977 and 2022 on online databases (including PubMed, CNKI, SciFinder, and Web of Science) and other sources (such as the Chinese Pharmacopoeia 2020 edition, and Chinese herbal books). Results: The genus Polygonatum contains 79 species, and 233 bioactive chemical compounds were identified in them. The abundance of pharmacological activities, such as antioxidant activities, anti-fatigue activities, anti-inflammatory activities, etc., were revealed for the representatives of this genus. In addition, there are numerous processing methods, and many chemical constituents and pharmacological activities change after the unappropriated processing. Conclusions: This review summarizes the taxonomy classification, chemical composition, pharmacological effects, and processing of the plants belonging to the genus Polygonatum, providing references and research tendencies for plant-based drug development and further clinical applications.
摘要：
Objective: Exploring the feasibility of combining herbal fumigation and myoelectric biofeedback therapy in injured players with post-stroke shoulder -hand syndrome. Methods: A total of 80 players with shoulder-hand syndrome after stroke who were healed in our hospital from July 2019 to June 2021 were retrospectively opted as the research subjects, and were divided into a joint intervention cluster (JIG) according to the variations in their healing methods. cluster, n=40, receiving traditional Chinese medicine fumigation and EMG biofeedback healing) and EMG healing cluster (Electromyobiological feedback cluster, EFG cluster, n=40), the healing effect, changes in simplified FMA mark of upper limbs before and after healing, and healing effects were contrasted between the two clusters. The changes of the front and rear shoulder pain and the pain part of the High Coast Shoulder Joint Function Rating Scale were recorded, and the occurrence of adverse reactions in the two clusters of injured players was recorded; Results: The total effective rate of injured players in the JIG cluster was 97.50% (39/40), which was notably upper than 85.00% (34/40) in the EFG cluster, and the variation between the clusters was notable (P<0.05). None notable variation in the simplified FMA mark between the clusters (P>0.05). On the 7th, 14th, and 28th days of healing, the simplified FMA mark of the upper limbs of the JIG cluster was notably upper than that of the EFG cluster, and the variation was notable (P>0.05). P<0.05); before healing, None notable variation between the two clusters in the degree of shoulder pain and the pain part of the Gaoshore Shoulder Joint Function Assessment Scale (P>0.05). After 28 days of healing, the degree of shoulder pain in the JIG cluster was notably bottom In the EFG cluster, the pain mark of the Gaoan Shoulder Joint Function Assessment Scale was notably upper than that in the EFG cluster, and the variation between the two clusters was notable (P<0.05); None notable variation in the incidence of adverse reactions between the two clusters (P >0.05); Conclusion: Combination of traditional Chinese medicine fumigation and EMG biofeedback for injured players with shoulder-hand syndrome after stroke has a good effect, which can notably relieve the pain symptoms of injured players, enhance their joint mobility, and at the same time, the healing is safe.
