摘要：
Triple-negative breast cancer (TNBC) metastasis is the cause of nearly 90% of breast cancer treatment failures as the side effects of clinical drugs and insufficient efficacy of single-modality therapy. Shikonin (SHK) is a naphthoquinone natural product from the herbal medicine Lithospermum erythrorhizon., which is widely used for cancer therapy as one kind of Chinese medicine for its significant anti-tumor, anti-metastatic, and anti-inflammatory effects. However, passive targeting of SHK to tumor location in vivo and long-term high dose administration is likely to decrease heart rate and induce drug resistance, which accordingly results in therapeutic failure. Thus, we designed a biomimetic SHK-loading nano-system (Hybrid membrane@ICG/PEI@HPB@SHK nanoparticles, HMGPHS NPs) to achieve controlled release and reduced SHK dosage. The prolonged circulation time and higher targeting of the HMGPHS NPs allowed less drug leakage systemically with increased drug concentration at the tumor sites compared to naked ICG/PEI@HPB@SHK (GPHS) NPs. Moreover, SHK could down-regulate the expression of COX-2, IL-6, and TNF-alpha to reduce the photothermal therapy-induced inflammatory response and improve the tumor microenvironment (TME), enabling HMGPHS NPs to significantly inhibit the growth and metastasis of TNBC. We propose that the combination of high efficacy, prolonged circulation, and high targeting of HMGPHS NPs could facilitate the conversion of SHK to clinical application for the effective treatment of TNBC. (C) 2022 The Authors. Published by Elsevier Ltd.
摘要：
Nonsmall-cell lung carcinoma (NSCLC) is one of the deadliest malignancies in the world. LncRNAs are confirmed to be involved in the progression of NSCLC. Meanwhile, lncRNA CRNDE is known to be upregulated in NSCLC; however, the mechanism by which CRNDE regulates the tumourigenesis of NSCLC remains unclear. To test the function of CRNDE in NSCLC, cell proliferation, invasion, and migration were investigated by colony formation and Transwell assays, respectively. qPCR and Western blotting were applied to test gene and protein levels. In addition, the relationship among CRNDE, miR-455-3p, and HDAC2 was explored by dual-luciferase and RIP assays. The data revealed that the expression of CRNDE was upregulated in NSCLC tissues, while miR-455-3p was downregulated. CRNDE knockdown inhibited the viability, migration and invasion of NSCLC cells or epidermal growth factor receptor gene (EGFR)-mutant NSCLC cells. Moreover, inhibition of miR-455-3p exhibited the opposite effect. CRNDE bound with miR-455-3p, and HDAC2 was found to be targeted by miR-455-3p. Meanwhile, miR-455-3p downregulation reversed the effect of CRNDE knockdown on NSCLC cell function. Furthermore, miR-455-3p notably inhibited the growth and invasion of NSCLC cells via downregulation of HDAC2. Knockdown of CRNDE attenuated NSCLC progression via modulation of the miR-455-3p/HDAC2 axis. Thus, those findings might provide a novel strategy against NSCLC.
期刊：
American Journal of Chinese Medicine,2022年51(01):107-127 ISSN：0192-415X
通讯作者：
Wei-Hua Huang
作者机构：
[Huang, Wei-Hua; Wang, Li; Zhang, Wei; Wang, Lin; Chen, Man-Yun] Cent South Univ, Dept Clin Pharmacol, Changsha 410078, Hunan, Peoples R China.;[Huang, Wei-Hua; Wang, Li; Zhang, Wei; Wang, Lin; Chen, Man-Yun] Cent South Univ, Inst Clin Pharmacol, Hunan Key Lab Pharmacogenet, Changsha 410078, Hunan, Peoples R China.;[Huang, Wei-Hua; Wang, Li; Zhang, Wei; Wang, Lin; Chen, Man-Yun] Cent South Univ, Xiangya Hosp, Natl Clin Res Ctr Geriatr Disorders, Xiangya Rd 110, Changsha 410008, Hunan, Peoples R China.;[Shao, Li] Cent South Univ, Xiangya Hosp, Dept Gen Surg, Xiangya Rd 110, Changsha 410008, Hunan, Peoples R China.;[Yang, Pu; Tan, Feng-Bo] Hunan Univ Chinese Med, Sch Pharm, Dept Pharmacognosy, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Wei-Hua Huang] D;Department of Clinical Pharmacology, Hunan Key Laboratory of Pharmacogenetics, Xiangya Hospital, Central South University, Changsha, Hunan 410078, P. R. China<&wdkj&>Institute of Clinical Pharmacology, Hunan Key Laboratory of Pharmacogenetics Xiangya Hospital, Central South University, Changsha, Hunan 410078, P. R. China<&wdkj&>National Clinical Research Center for Geriatric Disorders, School of Pharmacy, Hunan University of Chinese Medicine, Changsha, Hunan 410128, P. R. China
摘要：
Gut microbiota are significantly associated with the occurrence and development of inflammatory bowel disease (IBD). Panax notoginseng saponins (PNS) could be used for colitis and to modulate gut microbiota. However, the mechanism behind the effects of PNS on anti-colitis that are pertinent to gut microbiota is largely unknown. This study aimed to evaluate the anti-colitis effects of PNS and explore the involved mechanism as it is related to gut microbiota. Results showed that PNS significantly alleviated dextran sulfate sodium (DSS)-induced colitis. Meanwhile, after PNS treatment, the tight junction proteins were enhanced and proinflammatory cytokines, such as TNF-[Formula: see text], IL-6, IL-1[Formula: see text], and IL-17, were decreased. Furthermore, Bacteroides spp. were significantly increased after modeling, while PNS reduced their abundance and significantly increased the amount of Akkermansia spp. in vivo. Importantly, Akkermansia spp. and Bacteroides spp. were correlated with the IBD disease indicators. Moreover, fecal microbiota transplantation (FMT) experiments confirmed that PNS-reshaped gut microbiota significantly alleviated DSS-induced colitis, while A.muciniphila significantly reduced the levels of the LPS-induced cellular inflammatory factors IL-1[Formula: see text] and TNF-[Formula: see text]. In conclusion, PNS alleviated colitis pertinent to the upregulation of Akkermania spp. and downregulation of Bacteroides spp. in the gut.
摘要：
Purpose: To screen the hypoglycemic active ingredients from Coptidis rhizoma, and study their targets as well as signal pathways via network pharmacology.Methods: The fifty-nine ingredients of Coptidis rhizoma were screened for. Their targets were confirmed by comparing with the hypoglycemic targets in DrugBank databases. The relationship between ingredients and targets was revealed through String database. The ingredient-targetpassageway network was constructed. Coptidis rhizoma was soaked in boiling water and concentrated. Rat models were rendered diabetic by the administration of streptozotocin (STZ) intraperitoneal injection, and administered Coptidis rhizoma (0.40 g/kg, once a day by gavage), and tested for antidiabetic activity.Results: After four weeks of treatment, their blood glucose levels (BG) of all treated hyperglycemic rats decreased (p < 0.05). Twenty-four hypoglycemic compounds screened from Coptidis rhizome via network pharmacology could activate 13 targets, such as D (2) dopamine receptor (DRD2), insulin-like growth factor 1 receptor (IGF1R), 5-hydroxytryptamine receptor 2C (HTR2C), 5-hydroxytryptamine receptor 3A (HTR3A) and sodium-dependent noradrenaline transporter (SLC6A2). These targets were involved in 141 pathways, e.g., cAMP signaling pathway, chemokine signaling pathway, Rap1 signaling pathway, estrogen signaling pathway, and Apelin signaling pathway.Conclusion: This study suggests that Coptidis rhizoma contains several active compounds that show significant hypoglycemic effects. Furthermore, this study has established the basis for future investigations on the hypoglycemic effects of Coptidis rhizoma.
摘要：
Cordyceps is a large group of entomogenous, medicinally important fungi. In this study, we sequenced, assembled, and annotated the entire mitochondrial genome of Ophiocordyceps xuefengensis, in addition to comparing it against other three complete cordyceps mitogenomes that were previously published. Comparative analysis indicated that the four complete mitogenomes are all composed of circular DNA molecules, although their sizes significantly differ due to high variability in intron and intergenic region sizes in the Ophiocordyceps sinensis and O. xuefengensis mitogenomes. All mitogenomes contain 14 conserved genes and two ribosomal RNA genes, but varying numbers of tRNA introns. The Ka/Ks ratios for all 14 PCGs and rps3 were all less than 1, indicating that these genes have been subject to purifying selection. Phylogenetic analysis was conducted using concatenated amino acid and nucleotide sequences of the 14 PCGs and rps3 using two different methods (Maximum Likelihood and Bayesian analysis), revealing highly supported relationships between O. xuefengensis and other Ophiocordyceps species, in addition to a close relationship with O. sinensis. Further, the analyses indicated that cox1 and rps3 play important roles in population differentiation. These mitogenomes will allow further study of the population genetics, taxonomy, and evolutionary biology of medicinally important cordyceps species.
期刊：
Frontiers in Bioscience-Landmark,2022年27(9) ISSN：2768-6701
作者机构：
[Zeng, Li; Li, Keshuai; Xie, Han; Liang, Zimao; Zhu, Qianru; Zhai, Xiangyang; Huang, Xingxing; Wu, Qibiao] Macau Univ Sci & Technol, Fac Chinese Med, Taipa 999078, Macao, Peoples R China.;[Li, Keshuai; Xie, Han; Qiu, Zejing; Liang, Zimao; Zhu, Qianru; Sui, Xinbing; Zhai, Xiangyang; Huang, Xingxing] Hangzhou Normal Univ, Coll Pharm, Hangzhou 311121, Zhejiang, Peoples R China.;[Li, Keshuai; Xie, Han; Qiu, Zejing; Liang, Zimao; Zhu, Qianru; Sui, Xinbing; Zhai, Xiangyang; Huang, Xingxing] Hangzhou Normal Univ, Affiliated Hosp, Dept Med Oncol, Hangzhou 310015, Zhejiang, Peoples R China.;[Cheng, Haibo; Shen, Weixing] Nanjing Univ Chinese Med, Jiangsu Collaborat Innovat Ctr Tradit Chinese Med, Clin Med Coll 1, Nanjing 210023, Jiangsu, Peoples R China.;[Shao, Le] Hunan Univ Chinese Med, Ctr Med Res & Innovat, Hosp 1, Changsha 410000, Hunan, Peoples R China.
关键词：
natural compounds;conventional therapeutics;colorectal cancer;progression and metastasis;drug combination
摘要：
Cancer progression and metastases are the leading causes of poor outcomes in patients with colon cancer. Colon cancer metastasis is a multigene, multistep, multistage complex process in which target genes, microRNAs, epithelial-stromal transformation, tumour stem cells, the tumour microenvironment, and various cell signalling pathways are implicated in the progression and metastasis of colon cancer. Although conventional therapies have made significant advances in treating the progression and metastasis of colorectal cancer, they have failed to improve survival outcomes. Natural compounds may have more significant potential in preventing and treating colon cancer. Active natural compounds exert their antitumor effects by inducing tumour cell differentiation, promoting tumour cell apoptosis, inhibiting tumour vascular growth, and regulating immunity. Natural compounds, combined with conventional therapies, can target mutant genes and various cellular signalling pathways, inhibit epithelial-stromal transformation, and improve the tumour microenvironment to inhibit tumour progression and metastasis. The synergism of natural compounds and conventional therapeutics has the potential to become a promising therapy for treating colorectal cancer progression and metastases.
摘要：
Background: Inhaled formulations are the first choices for treating asthma and chronic obstructive pulmonary disease (COPD), attracting the increasing investment and development in the pharmaceutical industry. Both the equivalence of local and systemic exposures need to be considered when assessing the equivalence of generic inhaled drugs, which has become a dilemma in the development of generic inhaled drugs. There is an urgent need for reliable methods such as physiologically-based pharmacokinetic (PBPK) model to assist in the development of inhaled drugs.Method: To test the strategy that in silico simulation is an effective tool in developing inhaled products and further assessing their clinically feasibility, a long-acting beta2-adrenergic agonists indacaterol, which was referred as the first-line therapy for patient with COPD, was selected as a tool drug. The PBPK model was established and the predicted plasma concentration curve was obtained by inputting the physicochemical properties of indacaterol and adjusting model parameters. The accuracy of simulation was verified by an alignment with the actual data. The main factor affecting PK in vivo was investigated by parameter sensitivity analysis. The biological equivalent size of indacaterol was investigated by virtual bioequivalence analysis.Results: The models of indacaterol after intravenous and oral administration were established and confirmed, and used as a background for PBPK model of inhaled administration. All those models showed favorable stability and applicability. Appropriate lung deposition was generated in the PBPK model, and the predicted plasma profile of indacaterol was consistent with the clinical actual observation values. Particle size is the most important factor affecting the PK of indacaterol in vivo. Furthermore, virtual bioequivalence simulation exhibited statistically comparable results between the particle size fluctuates in the range of 3.5-6.5 mu m and baseline levels (D90 = 5 mu m).Conclusions: The PBPK model can simulate the pharmacokinetics and lung deposition of indacaterol, which will be a powerful tool to assist the development of inhaled drugs.
作者机构：
[Zou, Junju; Yu, Rong] Hunan Univ Chinese Med, Hunan Prov Key Lab Translat Res TCM Prescript & Z, 300 Xueshi Rd, Changsha 410208, Hunan, Peoples R China.;[Zhou, Xiaotao; Ma, Yuerong] Chengdu Univ Chinese Med, Sch Basic Med, Chengdu 611137, Peoples R China.
通讯机构：
[Yu, R.] H;Hunan Provincial Key Laboratory of Translational Research in TCM Prescriptions and Zheng, 300 Xueshi Road, Yuelu District, Hunan, China
关键词：
memory disorder;meta-analysis;protocol;Repetitive transcranial magnetic stimulation;stroke
摘要：
Background: Approximately 23% to 55% of patients have memory impairments with a greatly negative effect on daily life 3 months after stroke. Repetitive transcranial magnetic stimulation (rTMS) has been widely used in the rehabilitation of stroke as it is safe, painless, and noninvasive. Moreover, few studies have investigated the effect of rTMS on poststroke memory disorder (PSMD). However, the efficacy of rTMS is not consistent and the optional stimulation frequency is unclear. Therefore, this protocol aims to evaluate the clinical effect and safety of rTMS on PSMD by analyzing results from randomized controlled trials. Methods: Search strategies will be performed on seven databases: PubMed, EMBASE, CENTRAL, Chinese Biomedical Literature Database (CBM), Chinese National Knowledge Infrastructure (CNKI), Wan Fang, and Technology Periodical Database (VIP). Only randomized controlled trials registered before August 2021 will be included. Additionally, the language will be limited to English or Chinese. For the outcome, we will focus on the Rivermead Behavioral Memory Test. Additionally, the Montreal Cognitive Assessment, Mini-mental State Examination, Modified Barthel Index, and advent events will be included. Two authors will independently select the study, extract data, and assess quality. Moreover, disagreements will be resolved by the third author. STATA 14 and Review Manager 5.4 will be used to perform the analysis. We will evaluate bias risk in accordance with the Cochrane Handbook for Systematic Reviews of Interventions. To assess the quality of evidence, the Grading of Recommendations Assessment, Development, and Evaluation method will be employed. Results: This study will provide a comprehensive analysis of the current evidence on rTMS for PSMD. Conclusion: A reliable conclusion regarding whether rTMS is an effective and safe intervention for patients with PSMD and the effect of stimulation frequency and sham stimulation will be provided. This study will provide new insights for TMS in treating PSMD, and offer appropriate treatmentoptions to patients and clinicians. PROSPERO registration number: CRD42021282439.
作者机构：
[Tang, Qianli; Liao, Xianjiu] Hunan Univ Chinese Med, Sch Integrated Chinese & Western Med, Changsha 410208, Hunan, Peoples R China.;[Li, Qingli; Tang, Qianli; Liao, Xianjiu] Youjiang Med Univ Nationalities, West Guangxi Key Lab Prevent & Treatment High Inc, Baise 533000, Guangxi, Peoples R China.;[Liu, Zhao; Qiu, Shang; Wu, Shengyue; Gao, Fenglei; Cai, Zhiheng; Ge, Kezhen] Xuzhou Med Univ, Sch Pharm, Jiangsu Key Lab New Drug Res & Clin Pharm, Xuzhou 221004, Jiangsu, Peoples R China.
通讯机构：
[Tang, Qianli] H;[Gao, Fenglei] X;Hunan Univ Chinese Med, Sch Integrated Chinese & Western Med, Changsha 410208, Hunan, Peoples R China.;Xuzhou Med Univ, Sch Pharm, Jiangsu Key Lab New Drug Res & Clin Pharm, Xuzhou 221004, Jiangsu, Peoples R China.
摘要：
Amyloid beta-peptide oligomer (A beta O) has received extensive attention from researchers because of its clinical therapeutic intervention targets and the value of reliable biological macromolecules markers for early diagnosis of Alzheimer's disease. We have developed a novel label-free electrochemical detection sensor for A beta O based on hybridization chain reaction (HCR)-triggered poly adenine to absorb silver nanoparticles (AgNPs). In this method, we first use the "capture probe" to immobilize the aptamer 1 on the surface of the gold electrode (GE) via poly adenine-Au. Next, aptamer 2 and A beta O were deposited on the electrode surface. The HCR process was initiated by the aptamer 2 fragment as a primer, producing a large number of long DNA sequences, which contained many adenines. Thereafter, the HCR product with long-repeated adenines could absorb many AgNPs on the surface of the electrode, which were used for subsequent electrochemical stripping of the AgNPs. The concentration range of the electrochemical signal of A beta O was 1 pMe10 nM, and the detection limit was 430 fM, which indicated that that the detection system has high selectivity for the target protein. (C) 2021 Elsevier B.V. All rights reserved.
作者机构：
[Li, Xing; Li, Ao; Yu, Xu-Dong; Zhou, Yu; He, Zhiming; Wang, Le; Shu, Xinhua] Shaoyang Univ, Sch Basic Med Sci, Shaoyang 422000, Peoples R China.;[Tan, Zhoujin; Li, Xiao-Ya] Hunan Univ Chinese Med, Coll Chinese Med, Changsha 410208, Hunan, Peoples R China.;[Shu, Xinhua; Reilly, James] Glasgow Caledonian Univ, Dept Biol & Biomed Sci, Glasgow City G4 0BA, Scotland.;[Xiao, Zhi-Yong] Univ South China, Affiliated Hosp 1, Hengyang Med Sch, Dept Crit Care Med, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Zhi-Yong Xiao] T;[Xinhua Shu] S;The First Affiliated Hospital,Department of Critical Care Medicine,Hengyang Medical School, University of South China, 421001, Hengyang, Hunan, China<&wdkj&>School of Basic Medical Sciences, Shaoyang University, 422000, Shaoyang, China<&wdkj&>Department of Biological and Biomedical Sciences, Glasgow Caledonian University, G4 0BA, Glasgow, United Kingdom
摘要：
Trans-urocanic acid (trans-UCA) is an isomer of cis-UCA and is widely distributed in the brain, predominantly in the hippocampus and prefrontal cortex. Previous studies have investigated the role of trans-UCA in non-spatial memory; however, its influence on spatial memory remains unclear. In the present study, network pharmacology strategy and behavioral testing were used to evaluate the role of trans-UCA in spatial memory and predict its possible mechanism. The results showed that there are 40 intersecting targets between trans-UCA and spatial memory identified by several databases and Venn diagram, indicating that trans-UCA may be involved in spatial memory. Behavioral results show that trans-UCA facilitates spatial working memory in the Y-maze test as well as spatial recognition memory acquisition, consolidation and retrieval in an object location recognition (OLR) task. Furthermore, PPI (protein-protein interaction) network analysis, GO (gene ontology) and KEGG (Kyoto encyclopedia of genes and genomes) pathway enrichment analyses show that the molecular mechanisms underlying the enhancing effect of trans-UCA on spatial memory are mainly associated with the regulation of insulin, mitogen-activated protein kinase (MAPK) and nuclear factor Kappa B (NF-κB) signaling pathways, serotonergic synapse and arginine and proline metabolism. The results of this study suggest that trans-UCA facilitates spatial memory in the Y-maze test and OLR task and may offer therapeutic potential for Alzheimer's disease (AD). The underlying mechanisms predicted by network pharmacology should be further verified.
摘要：
Abstract: Featured Application Authors are encouraged to provide a concise description of the specific application or a potential application of the work. This section is not mandatory. Abstract It is an essential measure for workers to wear safety helmets when entering the construction site to prevent head injuries caused by object collision and falling. This paper proposes a lightweight algorithm for helmet-wearing detection based on YOLOV5, which is faster and more robust for helmet detection in natural construction scenarios. In this paper, the MCA attention mechanism is embedded in the backbone network to help the network extract more productive information, reduce the missed detection rate of small helmet objects and improve detection accuracy. In order to ensure the safety of workers in construction, it is necessary to detect whether the construction workers are wearing safety helmets in real-time to achieve monitoring on-site. A channel pruning strategy is proposed on the MCA-YOLOv5 algorithm to compress it, realizing the optimal large-scale model into ultrasmall models for real-time detection on embedded or mobile devices. The experimental results on the public data set show that the model parameter volume is reduced by 87.2%, and the detection speed is increased by 53.5%, even though the MCA-YOLOv5-light reduces the mAP slightly. Keywords: helmet-wearing detection; YOLOv5; attention mechanism; model pruning
通讯机构：
[Zhixiong Liu; Quan Cheng] D;Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha, China<&wdkj&>National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, China<&wdkj&>Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha, China<&wdkj&>National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, China
摘要：
Chimeric antigen receptor (CAR) T cell (CAR-T cell) therapy based on gene editing technology represents a significant breakthrough in personalized immunotherapy for human cancer. This strategy uses genetic modification to enable T cells to target tumor-specific antigens, attack specific cancer cells, and bypass tumor cell apoptosis avoidance mechanisms to some extent. This method has been extensively used to treat hematologic diseases, but the therapeutic effect in solid tumors is not ideal. Tumor antigen escape, treatment-related toxicity, and the immunosuppressive tumor microenvironment (TME) limit their use of it. Target selection is the most critical aspect in determining the prognosis of patients receiving this treatment. This review provides a comprehensive summary of all therapeutic targets used in the clinic or shown promising potential. We summarize CAR-T cell therapies’ clinical trials, applications, research frontiers, and limitations in treating different cancers. We also explore coping strategies when encountering sub-optimal tumor-associated antigens (TAA) or TAA loss. Moreover, the importance of CAR-T cell therapy in cancer immunotherapy is emphasized.
期刊：
Frontiers in Cardiovascular Medicine,2022年9:2484 ISSN：2297-055X
作者机构：
[Zhu, Ying; Liu, Yaxuan; Shangguan, Xueli; Zou, Menglong; Long, Dan] Hunan Univ Chinese Med, Hosp 1, Changsha, Peoples R China.;[Wang, Xindong; Mao, Chenhan] Nanjing Univ Chinese Med, Clin Med Coll 3, Nanjing, Peoples R China.;[Zhang, Xinyue] Shandong Univ Tradit Chinese Med, Coll Clin Med 1, Jinan, Peoples R China.;[Wang, Xindong] Nanjing Univ Chinese Med, Affiliated Hosp Integrated Tradit Chinese & Wester, Nanjing, Peoples R China.
关键词：
Gut Microbiota;coronary heart disease;bibliometric analysis;visual analysis;Trimethylamine-n-oxide
摘要：
BackgroundExisting studies have indicated that gut microbiota is closely related to the occurrence and development of coronary heart disease(CHD). Gut microbiota and its metabolites may be important diagnostic markers for CHD in the future and are expected to become new targets for the prevention and treatment of CHD. However, the current studies exploring the link between CHD and gut microbiota are miscellaneous and poorly targeted, without bibliometric analysis available. ObjectiveThe purpose of this research was to perform a bibliometric and visual analysis of published papers on the relationship between CHD and gut microbiota. The study also sought to identify principal authors, institutions, and countries to analyze the research status and trends of gut microbiota research in the field of CHD. MethodsThe Web of Science Core Collection (WoSCC) database was searched for publications on CHD and gut microbiota between 2002 and 2022. CiteSpace 5.8. R1, VOSviewer 1.6.16, and Microsoft Excel 2019 software tools were utilized to perform this bibliometric analysis and visualization. ResultsThere were 457 qualified publications found in total, with the annual number of publications increasing. The United States dominated in this field. Hazen, Stanley l was the author of the most papers. Cleveland Clinic published the most papers of any institution. The six main clusters' specific characteristics were discovered through analysis of the co-occurrence of keywords: inflammation, diet, trimethylamine n-oxide, metabolism, cardiovascular disease, and myocardial infarction. Newly emerging research has focused predominantly on gut microbiota metabolites and recent strategies for intervention in coronary atherosclerosis. ConclusionThese results provided a useful perspective on current research and future prospects for the research on the link between CHD and gut microbiota, which may help researchers to select suitable collaborators and facilitate their research to elucidate the underlying molecular mechanisms of CHD, including the causes, prevention, and treatment.
