作者机构：
[Ying Wang; Qinghua Peng] Department of Ophthalmology, The First Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China;[Ying Deng] School of Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;[Yijing Yang] Hunan Provincial Key Laboratory for the Prevention and Treatment of Ophthalmology and Otolaryngology Diseases with Chinese Medicine, Changsha, Hunan 410208, China;Medical School, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;[Jing Lu] Hunan Provincial Key Laboratory for the Prevention and Treatment of Ophthalmology and Otolaryngology Diseases with Chinese Medicine, Changsha, Hunan 410208, China<&wdkj&>Medical School, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China
通讯机构：
[Yijing Yang] H;[Qinghua Peng] D;Hunan Provincial Key Laboratory for the Prevention and Treatment of Ophthalmology and Otolaryngology Diseases with Chinese Medicine, Changsha, Hunan 410208, China<&wdkj&>Department of Ophthalmology, The First Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China
摘要：
Plants from the Asteraceae family are widely used as ethno medicines to treatment parasitic, malaria, hematemesis, pruritus, pyretic, anthelmintic, wound healing. The aim of this review is to provide an overview of Asteraceae plants antimicrobial activity. The most relevant results from the published studies are summarized and discussed. The species in genus of Artemisia, Echinacea, Centaurea, Baccharis, and Calendula showed antimicrobial activity. Most of these species are usually used as ethno medicines to treat infection, inflammation, and parasitics. The effective part or component for antimicrobial was essential oil and crude extract, and essential oil attracted more attention. It was also reported that nanoparticles coated with crude extract were effective against multidrug resistant bacteria. For multidrug resistant bacteria study, the species in Armtemisia were the most investigated, and Staphylococcus aureus and Escherichia coli were the most studied multidrug resistant strains. The antimicrobial activity was evaluated mainly based on the results of minimum inhibitory concentration (MIC). Few reports have been reported on minimum bactericide concentration (MBC) and its antibacterial mechanisms. According to the reported study results, some plants in Asteraceae have the potential to be developed as bacteriostatic agents and against multidrug resistant bacteria. However, most studies are still in vitro, further clinical and applied studies are needed.
作者机构：
[Huang, Jiawang; Ma, Xinyue; Li, Ling; Liao, Zexuan; Liu, Zhuolin] Hunan Univ Chinese Med, Coll Integrated Tradit Chinese & Western Med, Changsha 410208, Peoples R China.;[Feng, Zhiying; Wang, Kangyu] Hunan Univ Chinese Med, Coll Tradit Chinese Med, Changsha 410208, Peoples R China.;[Ning, Yi; Lu, Fangguo] Hunan Univ Chinese Med, Med Sch, Changsha 410208, Peoples R China.;[Li, Ling; Liu, Zhuolin] Hunan Univ Chinese Med, Hunan Prov Key Lab Integrated Tradit Chinese & Wes, Changsha 410208, Peoples R China.
通讯机构：
[Li, L ] H;Hunan Univ Chinese Med, Coll Integrated Tradit Chinese & Western Med, Changsha 410208, Peoples R China.;Hunan Univ Chinese Med, Hunan Prov Key Lab Integrated Tradit Chinese & Wes, Changsha 410208, Peoples R China.
摘要：
Influenza is an acute viral respiratory infection that has caused high morbidity and mortality worldwide. Influenza A virus (IAV) has been found to activate multiple programmed cell death pathways, including ferroptosis. Ferroptosis is a novel form of programmed cell death in which the accumulation of intracellular iron promotes lipid peroxidation, leading to cell death. However, little is known about how influenza viruses induce ferroptosis in the host cells. In this study, based on network pharmacology, we predicted the mechanism of action of Maxing Shigan decoction (MXSGD) in IAV-induced ferroptosis, and found that this process was related to biological processes, cellular components, molecular function and multiple signaling pathways, where the hypoxia inducible factor-1(HIF-1) signaling pathway plays a significant role. Subsequently, we constructed the mouse lung epithelial (MLE-12) cell model by IAV-infected in vitro cell experiments, and revealed that IAV infection induced cellular ferroptosis that was characterized by mitochondrial damage, increased reactive oxygen species (ROS) release, increased total iron and iron ion contents, decreased expression of ferroptosis marker gene recombinant glutathione peroxidase 4 (GPX4), increased expression of acyl-CoA synthetase long chain family member 4 (ACSL4), and enhanced activation of hypoxia inducible factor-1 alpha (HIF-1 alpha), induced nitric oxide synthase (iNOS) and vascular endothelial growth factor (VEGF) in the HIF-1 signaling pathway. Treatment with MXSGD effectively reduced intracellular viral load, while reducing ROS, total iron and ferrous ion contents, repairing mitochondrial results and inhibiting the expression of cellular ferroptosis and the HIF-1 signaling pathway. Finally, based on animal experiments, it was found that MXSGD effectively alleviated pulmonary congestion, edema and inflammation in IAV-infected mice, and inhibited the expression of ferroptosis-related protein and the HIF-1 signaling pathway in lung tissues.
期刊：
Skin Research and Technology,2023年29(9) ISSN：0909-752X
通讯作者：
Li, X
作者机构：
[Li, Xiaosha; Wang, Haizhen] Hunan Univ Chinese Med, Affiliated Hosp 2, Dept Dermatol, Changsha, Peoples R China.;[Tang, Shiyang] Hunan Univ Chinese Med, Sch Chinese Med, Changsha, Peoples R China.;[Li, Xin] Hunan Univ Chinese Med, Hunan Prov Key Lab Diagnost Chinese Med, 300 Xueshi Rd, Hanpu Sci & Educ Pk, Changsha 410208, Hunan, Peoples R China.
通讯机构：
[Li, X ] H;Hunan Univ Chinese Med, Hunan Prov Key Lab Diagnost Chinese Med, 300 Xueshi Rd, Hanpu Sci & Educ Pk, Changsha 410208, Hunan, Peoples R China.
关键词：
NLRP3;pyroptosis;ROS;tanshinoneIIA;vitiligo
摘要：
Abstract Background Pyroptosis has been implicated in the development of human diseases, including vitiligo. TanshinoneIIA has been confirmed to play anti‐vitiligo role. However, whether tanshinoneIIA inhibits vitiligo progression via regulating cell pyroptosis remains unclear. Methods Hydrogen peroxide (H2O2)‐induced melanocytes were used to mimic vitiligo cell model in vitro. Cell viability was assessed by cell counting kit 8 assay, and reactive oxygen species (ROS) production was detected by DCFH‐DA staining. Nod‐like receptor protein 3 (NLRP3) expression was detected by quantitative real‐time PCR, western blot and immunofluorescence staining. Cell pyroptosis was measured using flow cytometry, and the contents of interleukin‐1β and interleukin‐18 were determined by ELISA. Besides, the protein levels of apoptosis‐associated speck‐like protein containing CARD (ASC) and cleaved‐Caspase‐1 were examined by western blot analysis. Results H2O2 could induce ROS production, NLRP3 expression and pyroptosis in melanocytes. TanshinoneIIA inhibited ROS production, pyroptosis, and the expression of NLRP3, ASC and cleaved‐caspase‐1 in H2O2‐induced melanocytes. Compared with the function of ROS inhibitor (NAC), tanshinoneIIA acted as a ROS scavenger to relieve melanocyte pyroptosis. In addition, NLRP3 inhibitor (MCC950) also could aggravate the inhibition effect of tanshinoneIIA on melanocyte pyroptosis. Conclusion TanshinoneIIA suppressed melanocyte pyroptosis probably through modulating the ROS/NLRP3 signaling axis, which provides the evidence for therapeutic effect in vitiligo.
摘要：
BACKGROUND: Spinal cord injury (SCI) refers to the interruption of the tracts inside the spinal cord caused by various factors. The repair of damaged axons has always been a difficult point in clinical treatment and neuroscience research. The treatment of SCI with Buyang huanwu decoction (BYHWD), a well-known recipe for invigorating Qi (a vital force forming part of any living entity in traditional Chinese culture) and promoting blood circulation, shows a good effect. METHODS: The rubrospinal tract (RST) transection model in rats was established in this study and rats were administrated with low (BL), medium (BM), or high (BH) doses of BYHWD. RESULTS: Compared with the SCI group, BL, BM moderately, and BH significantly improved the motor function of forelimbs and increased the number of red nucleus neurons in SCI rats. As for the possible molecular mechanism, BL, BM moderately, and BH significantly increased mTOR whereas decreased Beclin-1 and LC3 in the red nucleus. CONCLUSION: In conclusion, low, medium, and high doses of BYHWD could promote neural recovery in SCI rats through improving motor function and neuron survival in the red nucleus. The neuroprotective effects of BYHWD might be associated with affecting the mTOR signaling pathway and autophagy.
作者机构：
[Yu Yi-Pin; Sheng Dan; Zhong Li-Qin; Tan Duo-Ting] Hunan Univ Chinese Med, Grad Sch, Changsha 410208, Peoples R China.;[Liang, H; Hu Zhi-Xi; Liang Hao; Yang Liu] Hunan Univ Chinese Med, Inst TCM Diagnost, Changsha 410208, Peoples R China.;[Huang Ru-Jia] Hunan Univ Chinese Med, Sch Integrated Chinese & Western Med, Changsha 410208, Peoples R China.
通讯机构：
[Liang, H ] H;Hunan Univ Chinese Med, Inst TCM Diagnost, Changsha 410208, Peoples R China.
作者机构：
[肖凡; 黄雅兰; Lyu X.] School of Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha, 410208, China;General Hospital of Ningxia Medical University, Yinchuan, 750001, China;Hunan Provincial Key Laboratory of Translational Medicine, Hunan University of Chinese Medicine, Changsha, 410208, China;[吴勇军] School of Pharmacy, Hunan University of Chinese Medicine, Changsha, 410208, China;[刘秀; 喻嵘] School of Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha, 410208, China, Hunan Provincial Key Laboratory of Translational Medicine, Hunan University of Chinese Medicine, Changsha, 410208, China
通讯机构：
[Yu, R.] S;School of Traditional Chinese Medicine, China
