期刊：
Molecular medicine (Cambridge, Mass.),2010年16(9-10):438-449 ISSN：1076-1551
通讯作者：
Tang, CK
作者机构：
[Yin, Kai; Tang, Chao-ke] Univ S China, Inst Cardiovasc Res, Life Sci Res Ctr, Key Lab Atherosclerol Hunan Prov, Hengyang 421001, Hunan, Peoples R China.;[Liao, Duan-fang] Hunan Univ Chinese Med, Sch Pharm, Dept Tradit Chinese Diagnost, Changsha, Hunan, Peoples R China.
通讯机构：
[Tang, CK] Univ S China, Inst Cardiovasc Res, Life Sci Res Ctr, Key Lab Atherosclerol Hunan Prov, Hengyang 421001, Hunan, Peoples R China.
摘要：
Atherosclerosis is characterized by a chronic inflammatory condition that involves numerous cellular and molecular inflammatory components. A wide array of inflammatory mediators, such as cytokines and proteins produced by macrophages and other cells, play a critical role in the development and progression of the disease ATP-binding membrane cassette transporter A1 (ABCA1) is crucial for cellular cholesterol efflux and reverse cholesterol transport (RCT) and is also identified as an important target in antiatherosclerosis treatment Evidence from several recent studies indicates that inflammation, along with other atherogenic-related mediators, plays distinct regulating roles in ABCA1 expression Proatherogenic cytokines such as interferon (IFN)-gamma and interleukin (IL)-1 beta have been shown to inhibit the expression of ABCA1, while antiatherogenic cytokines, including IL-10 and transforming growth factor (TGF)-beta 1, have been shown to promote the expression of ABCA1 Moreover, some cytokines such as tumor necrosis factor (TNF)-alpha seem to regulate ABCA1 expression in species-specific and dose-dependent manners Inflammatory proteins such as C-reactive protein (CRP) and cyclooxygenase (COX)-2 are likely to inhibit ABCA1 expression during inflammation, and inflammation induced by lipopolysaccharide (LPS) was also found to block the expression of ABCA1 Interestingly, recent experiments revealed ABCA1 can function as an antiinflammatory receptor to suppress the expression of inflammatory factors, suggesting that ABCA1 may be the molecular basis for the interaction between inflammation and ROT. This review aims to summarize recent findings on the role of inflammatory cytokines, inflammatory proteins, inflammatory lipids, and the endotoxin-mediated inflammatory process in expression of ABCA1. Also covered is the current understanding of the function of ABCA1 in modulating the immune response and inflammation through its direct and indirect antiinflammatory mechanisms including lipid transport, high-density lipoprotein (HDL) formation and apoptosis. (C) 2010 The Feinstein Institute for Medical Research, www.feinsteininstitute.org
摘要：
P>1. Adipocyte hypertrophy and hyperplasia are important processes in the development of obesity. To understand obesity and its associated diseases, it is important to elucidate the molecular mechanisms governing adipogenesis. MicroRNA-375 has been shown to inhibit differentiation of neurites, and participate in the regulation of insulin secretion and blood homeostasis. However, it is unknown whether miR-375 plays a role in adipocyte differentiation. 2. To investigate the role of miR-375 in adipocyte differentiation, we compared the miR-375 expression level between 3T3-L1 pre-adipocytes and adipocytes using miRNA microarray and quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) analysis. Furthermore, we evaluated the effects of overexpression or inhibition of miR-375 on 3T3-L1 adipocyte differentiation. 3. In the present study, we found that miR-375 expression was increased after induction of adipogenic differentiation. Overexpression of miR-375 enhanced 3T3-L1 adipocyte differentiation, as evidenced by its ability to increase mRNA levels of both CCAAT/enhancer binding protein-alpha (C/EBP alpha) and peroxisome proliferator-activated receptor-gamma (PPAR gamma 2), and induction of adipocyte fatty acid-binding protein (aP2) and triglyceride (TG) accumulation. Furthermore, we found overexpression of miR-375 suppressed phosphorylation levels of extracellular signal-regulated kinases 1/2 (ERK1/2). In contrast, anti-miR-375 increased ERK1/2 phosphorylation levels and inhibited mRNA expression of C/EBP alpha, PPAR gamma 2 and aP2 in 3T3-L1 adipocyte, accompanied by decreased adipocyte differentiation. 4. Taken together, these data suggest that miR-375 promotes 3T3-L1 adipocyte differentiation, possibly through modulating the ERK-PPAR gamma 2-aP2 pathway.
摘要：
Apelin is the endogenous ligand of the G protein-coupled receptor, APJ. Vascular smooth muscle cells express both apelin and APJ, which are important regulatory factors in the cardiovascular and nervous systems. Importantly, APJ is also involved in the pathogenesis if HIV-1 infection. We investigated whether vascular smooth muscle cell proliferation was regulated through an apelin-pERK1/2-cyclin D1 signal transduction pathway. Apelin-13 significantly stimulated vascular smooth muscle cell proliferation and increased cell cycle progression. Apelin-13 a decreased the proportion of cell in the G0/G1 phase while increasing the number of cells in S phase. Apelin-13 also increased the levels of cyclin D1, cyclin E and pERK1/2. Treatment of cells with the MEK inhibitor PD98059 attenuated the apelin-3-induced pERK1/2 activation. Similarly, treatment with PD98059 partially diminished the apelin-13-induced expression of cyclin D1 and vascular smooth muscle cell proliferation. Taken together, these data established that apelin-13 stimulates vascular smooth muscle cell proliferation by promoting the G1-S phase transition, and that this effect is mediated in part by an apelin-pERK1/2-cyclin D1 signal cascade.
摘要：
Aldo-keto reductase 1B10 (AKR1B10) is a secretory protein that is upregulated with tumorigenic transformation of human mammary epithelial cells. This study demonstrated that AKR1B10 was overexpressed in 20 (71.4%) of 28 ductal carcinomas in situ, 184 (83.6%) of 220 infiltrating carcinomas and 28 (87.5%) of 32 recurrent tumors. AKR1B10 expression in breast cancer was correlated positively with tumor size (p = 0.0012) and lymph node metastasis (p = 0.0123) but inversely with disease-related survival (p = 0.0120). Univariate (p = 0.0077) and multivariate (p = 0.0192) analyses both suggested that AKR1B10, alone or together with tumor size and node status, is a significant prognostic factor for breast cancer. Silencing of AKR1B10 in BT-20 human breast cancer cells inhibited cell growth in culture and tumorigenesis in female nude mice. Importantly, AKR1B10 in the serum of breast cancer patients was significantly increased to 15.18 +/- 9.08 ng/ml [n = 50; 95% confidence interval (CI), 12.6017.76], with a high level up to 58.4 ng/ml, compared to 3.34 +/- 2.27 ng/ml in healthy donors (n = 60; 95% CI, 2.783.90). In these patients, AKR1B10 levels in serum were correlated with its expression in tumors (r = 0.8066; p < 0.0001). Together our data suggests that AKR1B10 is overexpressed in breast cancer and may be a novel prognostic factor and serum marker for this deadly disease.
期刊：
Journal of ethnopharmacology,2009年121(3):412-418 ISSN：0378-8741
通讯作者：
Deng, CQ
作者机构：
[Deng, Chang-Qing; Liang, Yan; Zhang, Shu-Ping] Hunan Univ Tradit Chinese Med, Lab Pathophysiol, Changsha, Hunan, Peoples R China.;[Chen, Bei-Yang; Li, Hua] Hunan Univ Tradit Chinese Med, Dept Histol & Embryol, Changsha, Hunan, Peoples R China.;[Luo, Xue-Gang; Li, Hua] Cent S Univ, Xiangya Sch Med, Dept Anat & Neurobiol, Changsha, Hunan, Peoples R China.
通讯机构：
[Deng, CQ] Hunan Univ Tradit Chinese Med, Lab Pathophysiol, Changsha, Hunan, Peoples R China.
关键词：
Total saponins of Panax notoginseng;Cerebral ischemia-reperfusion;TUNEL;Caspase-1;Caspase-3;Caspase-8
摘要：
Ethnopharmacological relevance: Total saponins of Panax notoginseng (TSPN), main constituents extracted from Panax Notoginseng, a highly valued traditional Chinese medicine, have been shown to be an effective agent on cerebral infarction. Aim of the study: The effects of TSPN on apoptosis and expressions of caspase-1, caspase-3 and caspase-8 were studied after cerebral ischemia for 2 h followed by reperfusion for 46 h in rats. Materials and methods: Rats were subjected to transient middle cerebral artery occlusion (MCAO) model using the intraluminal thread. TSPN was administered intraperitoneally at 5 min before and 12 h, 24 h and 36 h after MCAO, respectively. Results: TSPN (at the dose of 25 mg/kg) significantly attenuated TUNEL-positive cells and reduced the expression of caspase-1 and caspase-3 compared to the model group, while it had no obvious effect on the expression of caspase-8. Conclusions: The neuroprotective effect of TSPN on focal ischemia may be related to inhibition of apoptosis and caspases activation. (c) 2008 Elsevier Ireland Ltd. All rights reserved.
作者：
Li Ying;Liang Fanrong;Yang Xuguang;Tian Xiaoping;Yan Jie;Sun Guojie;Chang Xiaorong;Tang Yong;Ma Tingting;Zhou Li;Lan Lei;Yao Wen;Zou Ran
期刊：
Headache,2009年49(6):805-816 ISSN：0017-8748
通讯作者：
Li, Y
作者机构：
[Li Ying; Ma Tingting; Tang Yong; Tian Xiaoping; Yang Xuguang; Liang Fanrong] Chengdu Univ Tradit Chinese Med, Chengdu, Sichuan, Peoples R China.;[Yao Wen; Lan Lei; Chang Xiaorong; Yan Jie] Hunan Univ Tradit Chinese Med, Changsha, Hunan, Peoples R China.;[Zhou Li; Sun Guojie; Zou Ran] Hubei Coll Tradit Chinese Med, Wuhan, Hubei, Peoples R China.
通讯机构：
[Li, Y] 37 Shi Er Qiao Rd, Chengdu 610075, Sichuan, Peoples R China.
关键词：
acupuncture therapy;acute migraine;acupuncture points;sham acupuncture;traditional Chinese medicine
摘要：
OBJECTIVE: To discuss the results of a multicenter randomized controlled trial of the efficacy of verum acupuncture in treating acute migraine attacks. BACKGROUND: Acupuncture has been used in China for centuries to treat migraine headache. Convincing evidence of its efficacy in alleviating pain, however, has been inadequate to date. METHODS: A total of 218 patients with migraine were recruited for the study; 180 met the inclusion criteria; 175 completed the callback process and were randomized into 3 groups. One group received verum acupuncture while subjects in the other 2 groups were treated with sham acupuncture. Each patient received 1 session of treatment and was observed over a period of 24 hours. The main outcome measure was the differences in visual analog scale (VAS) scores before treatment and 0.5, 1, 2, and 4 hours after treatment. RESULTS: Significant decreases in VAS scores from baseline were observed in the fourth hour after treatment when VAS was measured in the patients who received either verum acupuncture or sham acupunctures (P < .05). The VAS scores in the fourth hour after treatment decreased by a median of 1.0 cm, 0.5 cm, and 0.1 cm in the verum acupuncture group, sham acupuncture group 1, and sham acupuncture group 2, respectively. Similarly, there was a significant difference in the change in VAS scores from baseline in the second hour after treatment among the 3 groups (P = .006). Moreover, at the second hour after treatment, only patients treated with verum acupuncture showed significant decreases in VAS scores from baseline by a median of 0.7 cm (P < .001). Significant differences were observed in pain relief, relapse, or aggravation within 24 hours after treatment as well as in the general evaluations among the 3 groups (P < .05). Most patients in the acupuncture group experienced complete pain relief (40.7%) and did not experience recurrence or intensification of pain (79.6%). CONCLUSION: Verum acupuncture treatment is more effective than sham acupuncture based on either Chinese or Western nonacupoints in reducing the discomfort of acute migraine. Verum acupuncture is also clearly effective in relieving pain and preventing migraine relapse or aggravation. These findings support the contention that there are specific physiological effects that distinguish genuine acupoints from nonacupoints.
摘要：
Background: QiShenYiQi Pills (R) (QSYQ) is a compound Chinese medicine used in China for alleviating cardiac function. The present study was designed to explore the effect and mechanism of QSYQ on ischemia-reperfusion (I/R)-induced disorders in myocardial structure and function, with particularly focusing on the regulation of energy metabolism. Methods: Sprague-Dawley rats, with or without QSYQ pretreatment, were subjected to 30 min occlusion of the left anterior descending coronary artery and followed by 90 min or 24 h reperfusion. Myocardial blood flow (MBF) and cardiac function were evaluated at baseline, immediately after ischemia and 30, 60, 90 min, and 24 h after reperfusion. Myocardial infarction, myocardial histology and ultrastructure were assessed. Double staining of alpha-cardiac actinin and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling was conducted to assess myocardial apoptosis. ATP, ADP and AMP content was determined by Enzyme-Linked Immunosorbent Assay, F-actin in myocardial cells determined by immunofluorescence microscopy and expression of ATP synthase alpha, ATP5D, and phosphorylated-Myosin Light Chain (P-MLC) determined by western blotting. Results: Pre-treatment with QSYQ protected against I/R-induced MBF decrease, myocardial infarction and apoptosis at 90 min and 24 h after reperfusion. Moreover, I/R 90 min caused an impairment on cardiac function, a decrease in the ratio of ADP/ATP and AMP/ATP, accompanying with reduction of ATP 5D expression and increase in the expression of P-MLC, meanwhile, myocardium to exhibit myocardial fiber rupture, interstitial edema, and mitochondria swelling, all of which were significantly ameliorated by pre-treatment with QSYQ. Conclusions: The results of the present study suggest an involvement of regulation of energy metabolism in the action of QSYQ to protect against myocardial I/R injury. (C) 2012 Elsevier Ireland Ltd. All rights reserved.
摘要：
AKR1B10 (aldo-keto reductase family 1, member B10) protein is primarily expressed in normal human small intestine and colon, but overexpressed in several types of human cancers and considered as a tumour marker. In the present study, we found that AKR1B10 protein is secreted from normal intestinal epithelium and cultured cancer cells, as detected by a newly developed sandwich ELISA and Western blotting. The secretion of AKR1B10 was not affected by the protein-synthesis inhibitor cycloheximide and the classical protein-secretion pathway inhibitor brefeldin A, but was stimulated by temperature, ATP, Ca(2+) and the Ca(2+) carrier ionomycin, lysosomotropic NH(4)Cl, the G-protein activator GTP gamma S and the G-protein coupling receptor N-formylmethionyl-leucyl-phenylalanine. The ADP-ribosylation factor inhibitor 2-(4-fluorobenzoylamino)-benzoic acid methyl ester and the phospholipase C inhibitor U73122 inhibited the secretion of AKR1B10. In cultured cells, AKR1B10 was present in lysosomes and was secreted with cathepsin D, a lysosomal marker. In the intestine, AKR1B10 was specifically expressed in mature epithelial cells and secreted into the lumen at 188.6-535.7 ng/ml of Heal fluids (mean = 298.1 ng/ml, 17 = 11). Taken together, our results demonstrate that AKR1B10 is a new secretory protein belonging to a lysosome-mediated non-classical protein-secretion pathway and is a potential serum marker.
期刊：
World Journal of Gastroenterology,2009年15(25):3173-3177 ISSN：1007-9327
通讯作者：
Liang, FR
作者机构：
[Xiao-Ping; Yu; Fan-Rong; Ying; Ting-Ting; Yan] Chengdu Univ Tradit Chinese Med, Chengdu 610075, Sichuan, Peoples R China.;[Xiao-Rong; Jie] Hunan Univ Tradit Chinese Med, Changsha 410007, Hunan, Peoples R China.;[Sun] Hubei Coll Tradit Chinese Med, Wuhan 430065, Hubei, Peoples R China.
通讯机构：
[Liang, FR] Chengdu Univ Tradit Chinese Med, Chengdu 610075, Sichuan, Peoples R China.
关键词：
Nepean Dyspepsia Index;Functional dyspepsia;Health-related quality of life;Validation studies;Questionaires
摘要：
AIM: To assess the reliability and validity of the translated version of Nepean Dyspepsia Index (NDI) in Chinese patients with documented functional dyspepsia (FD).METHODS: The translation process included forward translation, back translation, pretest and cross-cultural adaptation. Reliability and validity of the translated version were examined by asking 300 subjects to complete the Chinese version of the NDI. The mean age of subjects was 39.24 years and 68.7% of the subjects were women. Internal consistency analysis with Cronbach's α was performed to test the reliability. Correlation analysis was used to assess the content validity. Factor analysis and structural equation models were used to assess the construct validity.RESULTS: The Cronbach's α coefficients ranged 0.833-0.960, well above the acceptable level of 0.70. Correlation analysis showed that each item had a strong correlation with the corresponding domain, but a weak correlation with other domains. Confirmatory factor analysis indicated that the comparative fit index was 0.94, higher than the acceptable level of 0.90.CONCLUSION: The Chinese version of the NDI is a reliable and valid scale for measuring health-related quality of life and disease severity in Chinese patients with FD.
作者机构：
[Zu, Xu-yu; Sun, Shao-wei; Tuo, Qin-hui; Lei, Xiao-yong; Liao, Duan-fang; Chen, Lin-xi] Univ S China, Inst Pharm & Pharmacol, Prov Key Lab Pharmacoprotom, Hengyang 421001, Peoples R China.;[Sun, Shao-wei; Liao, Duan-fang; Tang, Chao-ke] Univ S China, Life Sci Res Ctr, Key Lab Atherosclerol Hunan Prov, Hengyang 421001, Peoples R China.;[Liao, Duan-fang] Hunan Univ Chinese Med, Dept Tradit Chinese Diagnost, Sch Pharm, Changsha 410208, Hunan, Peoples R China.;[Li, Kai] Suzhou Univ, Mol Med Ctr, Suzhou 215004, Peoples R China.;[Li, Kai] Suzhou Univ, Affiliated Hosp 2, Suzhou 215004, Peoples R China.
通讯机构：
[Tang, CK] Univ S China, Life Sci Res Ctr, Key Lab Atherosclerol Hunan Prov, Hengyang 421001, Peoples R China.
关键词：
caveolae;caveolin-1;oxidized low density lipoprotein;atherosclerosis;transcytosis;human umbilical vein endothelial cells
摘要：
Aim: To explore the mechanisms involved in ox-LDL transcytosis across endothelial cells and the role of caveolae in this process. Methods: An in vitro model was established to investigate the passage of oxidized low density lipoprotein (ox-LDL) through a tight monolayer of human umbilical vein endothelial cells (HUVEC) cultured on a collagen-coated filter. Passage of Dil-labeled ox-LDL through the monolayer was measured using a fluorescence spectrophotometer. The uptake and efflux of ox-LDL by HUVEC were determined using fluorescence microscopy and HPLC. Results: Caveolae inhibitors - carrageenan (250 μg/mL), filipin (5 μg/mL), and nocodazole (33 μmol/L)-decreased the transport of ox-LDL across the monolayer by 48.9%, 72.4%, and 79.8% as compared to the control group. In addition, they effectively decreased ox-LDL uptake and inhibited the efflux of ox-LDL. Caveolin-1 and LOX-1 were up-regulated by ox-LDL in a time-dependent manner and decreased gradually after depletion of ox-LDL (P<0.05). After treatment HUVEC with ox-LDL and silencing caveolin-1, NF-KB translocation to the nucleus was blocked and LOX-1 expression decreased (P<0.05). Conclusion: Caveolae can be a carrier for ox-LDL and may be involved in the uptake and transcytosis of ox-LDL by HUVEC
期刊：
Phytomedicine : international journal of phytotherapy and phytopharmacology,2010年17(3-4):233-240 ISSN：0944-7113
通讯作者：
Deng, CQ
作者机构：
[Deng, Chang-Qing] Hunan Univ Tradit Chinese Med, Sch Integrated Chinese & Western Med, Pathophysiol Lab, Changsha, Hunan, Peoples R China.;[Chen, Bei-Yang; Zhang, Guo-Min; Li, Hua] Hunan Univ Tradit Chinese Med, Dept Pathol, Changsha, Hunan, Peoples R China.;[Tang, Ying-Hong] Hunan Univ Tradit Chinese Med, Dept Pharmacol, Changsha, Hunan, Peoples R China.;[Wu, Lu] Hunan Univ Tradit Chinese Med, Affiliated Tradit & Western Med Hosp 2, Changsha, Hunan, Peoples R China.
通讯机构：
[Deng, CQ] Hunan Univ Tradit Chinese Med, Sch Integrated Chinese & Western Med, Pathophysiol Lab, Xiangzui Rd, Changsha, Hunan, Peoples R China.
关键词：
Total saponins of "panax notoginseng root";Atorvastatin;Vascular smooth muscle cell;Proliferating cell nuclear antigen;Cyclind D(1);Cycline;Extracellular matrix;Collagen I;Fibronectin;Matrix metalloproteinase-9;Tissue inhibitor metalloproteinase-1
摘要：
Aim of the study: the effect of total saponins of "panax notoginseng root" on aortic intimal hyperplasia and the expressions of cell cycle protein and extracellular matrix in rats Materials and methods: Sprague-Dawley rats were randomly divided into sham-operated, control, TSPN and atorvastatin group. Rat aorta intima in all groups were injured by insertion of domestic balloon catheter into the aortae except sham-operated rats. Drugs were administrated orally from the second day after vascular injury and continued for 14 days. The injured segments of aortae were collected on the sixteenth day after operation to observe the morphological changes of vascular structure and to examine the expressions of proliferating cell nuclear antigen(PCNA), cyclinD1, cyclinE, collagen I(Col-I), fibronect(FN), matrix metalloproteinase-9(MMP-9) and tissue inhibitor metalloproteinase-1(TIMP-1). Results: TPNS significantly inhibited the vascular intimal hyperplasia. TPNS significantly lowered the expression of PCNA, cyclinE, cyclinD1, FN and MMP-9. TPNS had no significant impacts on the expression of Col-I and TIMP-1. Conclusions: Our studies indicated that TSPN could inhibit vessel restenosis after vascular intimal injury, and its mechanisms may be related to the blockage of the excessive proliferation of VSMC, the reduction of ECM protein deposition in the endometrium, and the degradation of ECM protein. (C) 2009 Elsevier GmbH. All rights reserved.
摘要：
Cholesterol efflux from lipid-loaded cells is a key athero-protective event that counteracts cholesterol uptake. The imbalance between cholesterol efflux and uptake determines the prevention or development of atherosclerosis. Many proteins and factors participate in the cholesterol efflux event. However, there are currently no systematic models of reverse cholesterol transport (RCT) that include most RCT-related factors and events. On the basis of recent research findings from other and our laboratories, we propose a novel model of one center and four systems with coupling transportation and networking regulation. This model represents a common way of cholesterol efflux; however, the systems in the model consist of different proteins/factors in different cells. In this review, we evaluate the novel model in vascular smooth muscle cells (VSMCs) and macrophages, which are the most important original cells of foam cells. This novel model consists of 1) a caveolae transport center, 2) an intracellular trafficking system of the caveolin-1 complex, 3) a transmembrane transport system of the C-A1 complex, 4) a transmembrane transport system of the SR-B1 complex, and 5) an extracelluar trafficking system of HDL/Apo-A1. In brief, the caveolin-1 system transports cholesterol from intracellular compartments to caveolae. Subsequently, both C-A1 and SR-B1 complex systems transfer cholesterol from caveolae to extracellular HDL/Apo-A1. The four systems are linked by a regulatory network. This model provides a simple and concise way to understand the dynamic process of atherosclerosis
摘要：
Ethnopharmacological relevance: Buyang Huanwu Decoction (BYHWD), a Chinese prescription that has been used for hundreds of years to treat paralysis, has gained attention recently due to its significant neuroprotective properties. Aim of the study: This study was to investigate whether BYHWD treatment protected axotomized rubrospinal neurons (RN) following spinal cord injury (SCI) in rats. Materials and methods: Adult rats received a right lateral funiculus transection at the level between C3 and C4, and were either treated with BYHWD or with distilled water (DW) via gastrogavage. Effects on RN viability and atrophy were determined by Nissl staining, axon regeneration was examined by biotinylated dextran amine (BDA) tracing techniques and functional recovery was studied by a test of forelimb usage during spontaneous vertical exploration. Results: RN cell number and mean somal size were 20% and 29% higher, respectively, in BYHWD-treated rats relative to DW-treated rats. There were a small number of BDA-labeled axons in the caudal of injury site in BYHWD-treated rats, whereas no caudal axonal regeneration was detected in DW-treated rats. BYHWD-treated rats used the injured forelimb more often than rats treated with DW. Conclusions: These results indicate that administration of BYHWD following SCI protects injured neurons, promotes regeneration, and enhances functional recovery. (C) 2008 Elsevier Ireland Ltd. All rights reserved.
期刊：
EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE,2013年 ISSN：1741-427X
通讯作者：
Wang, JB
作者机构：
[Zhao, Yan-Ling; Wang, Jia-Bo; Zeng, Ling-Na; Liu, Fei-Fei; Li, Qi; Pu, Shi-Biao; Ma, Zhi-Jie] 302 Mil Hosp, China Mil Inst Chinese Med, Beijing 100039, Peoples R China.;[Li, Jian-Yu; Bai, Yun-Feng; Xiao, Xiao-He; Wang, Rui-Lin; Yang, Hui-Yin] 302 Mil Hosp, Integrat Med Ctr, Beijing 100039, Peoples R China.;[Xiao, Da-Ke] China Pharmaceut Univ, Coll Pharm, Nanjing 211198, Jiangsu, Peoples R China.;[Xia, Xin-Hua] Hunan Univ Chinese Med, Coll Pharm, Changsha 410208, Hunan, Peoples R China.;[Zhong, Yan-Wei] 302 Mil Hosp, Pediat Liver Dis erapy & Res Ctr, Beijing 100039, Peoples R China.
通讯机构：
[Wang, JB] 302 Mil Hosp, China Mil Inst Chinese Med, Beijing 100039, Peoples R China.
摘要：
Some recent clinical reports have shown that the combination of oxymatrine, a phyto-derived drug, with lamivudine (3TC) could improve its curative effect against hepatitis B virus (HBV) infection. However, the experimental data in support of this combination strategy are lacking. In this study, we investigated the anti-HBV activity of the combination of 3TC and either oxymatrine or matrine on HepG2 2.2.15 in vitro. The activities of the combination and the solo compound, each in different concentrations, were compared on the 3rd, 6th, and 9th experimental days. The cytotoxicity results showed that the nontoxic concentrations of both oxymatrine and matrine to HepG2 2.2.15 cells were 800 mu g/mL. We found that the single use of oxymatrine below 100 mu g/ml, matrine below 200 mu g/ml, and 3TC below 30 mu g/ml showed weak inhibitory effects on the secretion of hepatitis B surface antigen (HBsAg), hepatitis B e antigen (HBeAg), and HBV-DNA in culture media; the combination of 3TC (30 mu g/ml) with oxymatrine (100 mu g/ml) or matrine (100 mu g/ml) showed significant inhibitory effects that were higher than or equivalent to the single use of 3TC at 100 mu g/ml. The results provide a new impetus to develop novel, multicomponent anti-HBV drugs through the combination of natural products with nucleoside analogs to enhance their activity.
期刊：
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY,2014年79:391-398 ISSN：0223-5234
通讯作者：
Luo, Zi-Qiang;Yin, Shuang-Feng
作者机构：
[Luo, Ning-Lin; Qiu, Ren-Hua; Yu, Kun; Tan, Nian-Yuan; Au, Chak-Tong; Chen, Yi] State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China;[Tong, Le; Chen, Yi] Medical College, Hunan University of Chinese Medicine, Changsha 410208, PR China;[Luo, Zi-Qiang] School of Basic Medicine, Central South University, Changsha 410013, PR China. Electronic address: luozq1962@163.com;[Liu, Wei; Chen, Yi] School of Basic Medicine, Central South University, Changsha 410013, PR China;[Yin, Shuang-Feng] State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China. Electronic address: sf_yin@hnu.edu.cn
通讯机构：
[Luo, Zi-Qiang] School of Basic Medicine, Central South University, Changsha 410013, PR China. Electronic address:;[Yin, Shuang-Feng] State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China. Electronic address:
摘要：
Three heterocyclic hypervalent organoantimony chlorides RN(CH2C6H4)(2)SbCI (2a R = t-Bu, 2b R = Cy, 2c R = Ph) and their chalcogenide derivatives [RN(CH2C6H4)(2)Sb](2)O (3a R = t-Bu, 3b R = Cy, 3c R = Ph) were synthesized and characterized by techniques such as H-1 NMR, C-13 NMR, X-ray diffraction, and elemental analysis. It is found that the anti-proliferative activity detected over these compounds can be attributed to the coordination bond between the antimony and nitrogen atoms of these compounds. Moreover, a preliminary study on mechanistic action suggests that the inhibition effect is ascribable to cell cycle arrest and cell apoptosis. (C)2014 Elsevier Masson SAS. All rights reserved.
作者机构：
[Tuo, Qinhui] Univ S China, Dept Pharmacol, Hengyang 421001, Peoples R China.;[Liao, Duan-Fang] Hunan Univ Chinese Med, Dept Tradit Chinese Diagnost, Sch Pharm, Changsha 410208, Hunan, Peoples R China.;[Chen, Jian-Xiong; Zeng, Heng] Univ Mississippi, Med Ctr, Dept Pharmacol & Toxicol, Jackson, MS 39216 USA.
通讯机构：
[Chen, JX] Univ Mississippi, Med Ctr, Dept Pharmacol & Toxicol, 2500 N State St, Jackson, MS 39216 USA.
摘要：
Diabetes is associated with impairment of angiogenesis such as reduction of myocardial capillary formation. Our previous studies demonstrate that disruption of Angiopoietin-1 (Ang-1)/Tie-2 signaling pathway contributes to the diabetes-associated impairment of angiogenesis. Protein tyrosine phosphatase (PTP) has a critical role in the regulation of insulin signal by inhibition of tyrosine kinase phosphorylation. In present study, we examined the role of protein tyrosine phosphatase-1 (SHP-1) in diabetes-associated impairment of Ang-1/Tie-2 angiogenic signaling and angiogenesis. SHP-1 expression was significantly increased in diabetic db/db mouse hearts. Furthermore, SHP-1 bond to Tie-2 receptor and stimulation with Ang-1 led to SHP-1 dissociation from Tie-2 in mouse heart microvascular endothelial cell (MHMEC). Exposure of MHMEC to high glucose (HG, 30 mmol/L) increased SHP1/Tie-2 association accompanied by a significant reduction of Tie-2 phosphorylation. Exposure of MHMEC to HG also blunted Ang-1-mediated SHP-1/Tie-2 dissociation. Knockdown of SHP-1 significantly attenuated HG-induced caspase-3 activation and apoptosis in MHMEC. Treatment with PTP inhibitors restored Ang-1-induced Akt/eNOS phosphorylation and angiogenesis. Our data implicate a critical role of SHP-1 in diabetes-associated vascular complications, and that upregulation of Ang-1/Tie-2 signaling by targeting SHP-1 should be considered as a new therapeutic strategy for the treatment of diabetes-associated impairment of angiogenesis.
作者：
Nagajyothi, Fnu;Kuliawat, Regina;Kusminski, Christine M.;Machado, Fabiana S.;Desruisseaux, Mahalia S.;Zhao, Dazhi;Schwartz, Gary J.;Huang, Huan;Albanese, Chris;Lisanti, Michael. P.;Singh, Rajat;Li, Feng;Weiss, Louis M.;Factor, Stephen M.;Pessin, Jeffrey E.;Scherer, Philipp E.;Tanowitz, Herbert B.
期刊：
AMERICAN JOURNAL OF PATHOLOGY,2013年182(3):886-894 ISSN：0002-9440
通讯作者：
Tanowitz, HB
作者机构：
[Pessin, Jeffrey E.; Schwartz, Gary J.; Singh, Rajat; Tanowitz, Herbert B.; Desruisseaux, Mahalia S.; Weiss, Louis M.] Albert Einstein Coll Med, Dept Med, Div Infect Dis, Bronx, NY 10461 USA.;[Pessin, Jeffrey E.; Schwartz, Gary J.; Singh, Rajat; Tanowitz, Herbert B.; Desruisseaux, Mahalia S.; Weiss, Louis M.] Albert Einstein Coll Med, Dept Med, Div Endocrinol, Bronx, NY 10461 USA.;[Pessin, Jeffrey E.; Schwartz, Gary J.; Singh, Rajat] Albert Einstein Coll Med, Diabet Res Ctr, Bronx, NY 10461 USA.;[Pessin, Jeffrey E.; Singh, Rajat] Albert Einstein Coll Med, Dept Mol Pharmacol, Bronx, NY 10461 USA.;[Lisanti, Michael. P.] Univ Manchester, Manchester Breast Ctr, Manchester, Lancs, England.
通讯机构：
[Tanowitz, HB] Albert Einstein Coll Med, Dept Pathol, 1300 Morris Pk Ave, Bronx, NY 10461 USA.
摘要：
Chagas disease, caused by Trypanosoma cruzi, is an important cause of morbidity and mortality primarily resulting from cardiac dysfunction, although T. cruziin fection results in inflammation and cell destruction in many organs. We found that T. cruzi (Brazil strain) infection of mice results in pancreatic inflammation and parasitism within pancreatic beta-cells with apparent sparing of a cells and leads to the disruption of pancreatic islet architecture, beta-cell dysfunction, and surprisingly, hypoglycemia. Blood glucose and insulin levels were reduced in infected mice during acute infection and insulin levels remained low into the chronic phase. In response to the hypoglycemia, glucagon levels 30 days postinfection were elevated, indicating normal a-cell function. Administration of L-arginine and a beta-adrenergic receptor agonist (CL316, 243, respectively) resulted in a diminished insulin response during the acute and chronic phases. Insulin granules were docked, but the lack of insulin secretion suggested an inability of granules to fuse at the plasma membrane of pancreatic beta-cells. In the liver, there was a concomitant reduced expression of glucose-6-phosphatase mRNA and glucose production from pyruvate (pyruvate tolerance test), demonstrating defective hepatic gluconeogenesis as a cause for the T. cruzi-induced hypoglycemia, despite reduced insulin, but elevated glucagon levels. The data establishes a complex, multi-tissue relationship between T. cruzi infection, Chagas disease, and host glucose homeostasis. (Am 3 Pathol 2013, 182:886-894; http:// dx.doi.org/10.1016/j.ajpath.2012.11.027)
作者机构：
[Yang, Xuguang; Zheng, Hui; Tian, Xiaoping; Yu, Shuguang; Li, Ying; Liu, Xuguang; Ma, Tingting; Tang, Yong; Liang, Fanrong] Chengdu Univ Tradit Chinese Med, Chengdu, Sichuan, Peoples R China.;[Yan, Jie; Chang, Xiaorong] Hunan Univ Tradit Chinese Med, Changsha, Hunan, Peoples R China.;[Sun, Guojie] Hubei Coll Tradit Chinese Med, Wuhan, Hubei, Peoples R China.;[Zhang, Hongxing] 1 Peoples Hosp Wuhan City, Wuhan, Hubei, Peoples R China.
通讯机构：
[Liang, FR] Chengdu Univ Tradit Chinese Med, Chengdu, Sichuan, Peoples R China.
摘要：
Background and motivation: The effectiveness of using acupuncture to treat migraine is rarely and even suspectedly reported in the literature. In this article, we report the design and the protocol of a randomized controlled large-scale trial to treat migraine using acupuncture, aiming at testifying it is effective to use acupuncture to treat migraine. We demonstrate also that the effectiveness of the treatment may vary due to using acupoints of different meridians or different acupoints of one meridian. Methods and design: A multi-center randomized controlled trial is currently undergoing, with three acupoints treatment groups and one non-acupoints control group. The acupuncture treatment consists of 20 sessions per patient with a observation period of 20 weeks. In total, 480 patients with Migraine are registered in this study within 8 hospitals in China from March 2008 to June 2009. These patients are randomly assigned to receive one of the following four acupoints treatment groups, i.e. 1) specific acupoints of Shaoyang meridians (120 patients), 2) non-specific acupoints of Shaoyang meridians (120 patients), 3) acupoints of other meridians (120 patients); or 4) non-acupoints control group (120 patients). The main outcome measurement in this trial is the effect comparison achieved among these four groups in terms of number of days with migraine and intensity of migraine during and after the baseline phase, i.e. the first 4 weeks before randomization and 4, 8 and 16 weeks after randomization. The intensity of headache including Headache intensity grade (0-3) and visual analogue scale (VAS) score will also be used in this study. In addition, the differences of Migraine-Specific Quality-of-Life Questionnaire (MSQ) and Transcranial Doppler Sonography (TCD) before and after randomization are also used as the secondary outcome measurement. Discussion: The result of this trial (which will be available in 2009) will demonstrate the efficacy of using acupuncture to treat migraine, and verify whether the specific effect of acupoints exists and whether this specific effect of acupoints is related to meridian and a collection of meridian Qi. Trials registration: Clinical Trials. gov NCT00599586
作者机构：
[Tao, Huai] Department of Biochemistry and Molecular Biology, Hunan University of Chinese Medicine, Changsha 410208, China;[Tang, Yamei; Tang, Aiguo; Li, Cunyan] Department of Laboratory, The Second Xiangya Hospital, Central South University, Changsha 410011, China;[Liu, Yong] Mental Health Institute, The Second Xiangya Hospital, Central South University, 139 Renmin Middle Road, Changsha 410011, China. Electronic address: csuliuyong@163.com;[Chen, Zhiheng] Department of Pediatrics, The Third Xiangya Hospital, Central South University, Changsha 410013, China;[Zhang, Xianghui] Mental Health Institute, The Second Xiangya Hospital, Central South University, 139 Renmin Middle Road, Changsha 410011, China
通讯机构：
[Liu, Yong] Mental Health Institute, The Second Xiangya Hospital, Central South University, 139 Renmin Middle Road, Changsha 410011, China. Electronic address:
摘要：
Oxytocin (OT) was reported to affect cognitive and emotional behavior by action in ventral tegmental area (VTA) and other brain areas. However, it is still unclear how OT activates VTA and related midline nucleus. Here, using patch-clamp recording, we studied the effects of OT on neuron activity in VTA and interfascicular nucleus (IF). OT dose-dependently and selectively excited small neurons located in medial VTA and the majority of IF neurons but not large neurons in lateral VIA. We found the hyperpolarization-activated current (I-h) and the membrane capacitance of OT-sensitive neuron were significantly smaller than those of OT-insensitive neurons. The action potential width of 01-sensitive neurons was about half that of OT-insensitive neurons. The OT effect was blocked by the OT receptor antagonist atosiban and WAY-267464 but not by tetrodotoxin, suggesting a direct postsynaptic activation of OT receptors. In addition, the phospholipase C (PLC) inhibitor U73122 antagonized the depolarization by OT. Both the nonselective cation channel (NSCC) antagonist SKF96365 and the Na+-Ca2+ exchanger (NCX) blocker SN-6 attenuated OT effects. These results suggested that the PLC signaling pathway coupling to NSCC and NCX contributes to the OT-mediated activation of neurons in medial VIA and IF. Taken together, our results indicate OT directly acted on medial WA and especially IF neurons to activate NSCC and NCX via PLC. The direct activation by OT of midbrain neurons may be one mechanism underlying OT effects on social behavior. (C) 2013 Elsevier Ltd. All rights reserved.
摘要：
OBJECTIVES: Total Panax notoginseng saponin (TPNS) is extracted from Panax notoginseng. Our previous studies suggested that TPNS could inhibit intimal hyperplasia. This study discussed the impact of TPNS on the proliferation of vascular smooth muscle cells (VSMCs) and revealed the associated mechanisms through cell cycle-related factors and extracellular regulated protein kinase (ERK) signal transduction pathway. METHODS: A VSMC proliferation model induced by platelet-derived growth factor (PDGF) was established to observe the effects of rat drug-containing plasma on VSMC proliferation. KEY FINDINGS: After being stimulated by PDGF, the proliferating cell nuclear antigen (PCNA) and c-fos content increased, while up-regulation of cyclinD1, cyclin-dependent kinase-4 (CDK4) and down-regulation of p21 protein were observed. These changes were inhibited by atorvastatin and TSPN drug-containing plasma, and the inhibitive activity in both groups was not significant. Furthermore, both atorvastatin and TSPN could obviously inhibit the activation of PDGF-induced P-ERK1/2 and increase the content of MKP-1, there were also no significant differences. CONCLUSIONS: These results suggested that atorvastatin and TPNS could inhibit VSMC proliferation by inhibiting the activation of ERK signalling pathway.
