摘要：
Heart failure (HF) is a complex syndrome marked by considerable expenditures and elevated mortality and morbidity rates globally. Shenmai injection (SMI), a form of Traditional Chinese Medicine-based therapy, has demonstrated effectiveness in treating HF. Recent research suggests that Traditional Chinese Medicine (TCM) may induce beneficial changes in microbial-host co-metabolism, potentially providing cardiovascular protection. This study used a rat model of hypertensive heart failure (H-HF) to explore the mechanism of SMI. The possible compounds and key targets of SMI against H-HF were investigated using network pharmacology. The pharmacodynamics of SMI were validated using the H-HF animal model, with analysis of fecal gut microbiota integrating metabolomics and 16S rRNA sequencing. Metorigin metabolite traceability analysis and the MetaboAnalyst platform were utilized to explore the action mechanism. To evaluate changes in serum TMAO levels, targeted metabolomics was performed. Finally, the study looked at the intrinsic relationships among modifications in the intestinal flora, metabolite profile changes, and the targets of SMI compounds to clarify how they might be used to treat H-HF. According to metabolomics and 16S rRNA sequencing, by reestablishing homeostasis in the gut microbiota, SMI affects vital metabolic pathways, such as energy metabolism, amino acid metabolism, and bile acid metabolism. Increased serum TMAO levels were identified to be a risk factor for H-HF, and SMI was able to downregulate the levels of TMAO-related metabolites. Network pharmacology analysis identified 13 active components of SMI targeting 46 proteins, resulting in differential expression changes in 8 metabolites and 24 gut microbes. In conclusion, this study highlights the effectiveness of SMI in alleviating H-HF and its potential to modulate microbial-host co-metabolism. Through a comprehensive discussion of the interconnected relationships among the components, targets, metabolites, and gut microbiota, it provided fresh light on the therapeutic mechanism of SMI on H-HF.
摘要：
Lycium barbarum (goji berry) is a globally valued species used as a health food and traditional medicine. It is known for its bioactive properties, including antioxidant, anti-aging, anticancer, and immune-enhancing effects. This review explores key aspects of L. barbarum research, focusing on its molecular biology, cultivation conditions, and quality characteristics. Advances in genetic studies have identified key genes linked to growth, stress resistance, and secondary metabolite biosynthesis. Cultivation practices, including environmental, irrigation and fertilization factors, significantly affect yield and fruit quality. Additionally, factors such as variety, developmental stage, and post-harvest processing influence the phytochemical composition and bioactive potential of goji berries. However, gaps remain in the comprehensive evaluation of all L. barbarum varieties and their quality attributes. Future studies should emphasize optimizing cultivation methods and refining processing techniques to enhance the functional and medicinal value of goji berries.
Lycium barbarum (goji berry) is a globally valued species used as a health food and traditional medicine. It is known for its bioactive properties, including antioxidant, anti-aging, anticancer, and immune-enhancing effects. This review explores key aspects of L. barbarum research, focusing on its molecular biology, cultivation conditions, and quality characteristics. Advances in genetic studies have identified key genes linked to growth, stress resistance, and secondary metabolite biosynthesis. Cultivation practices, including environmental, irrigation and fertilization factors, significantly affect yield and fruit quality. Additionally, factors such as variety, developmental stage, and post-harvest processing influence the phytochemical composition and bioactive potential of goji berries. However, gaps remain in the comprehensive evaluation of all L. barbarum varieties and their quality attributes. Future studies should emphasize optimizing cultivation methods and refining processing techniques to enhance the functional and medicinal value of goji berries.
摘要：
BACKGROUND: To explore the effects of Buyang Huanwu Decoction (BYHWD) on neurons in the red nucleus of rats with spinal cord injury (SCI) based on autophagy. METHODS: 120 Sprague-Dawley (SD) rats were randomly divided into 6 groups: Control Group, SCI Group, Bafilomycin A1 Group, Rapamycin Group, BYHWD low-dose group (BL Group, 6.25 g/kg), BYHWD high-dose group (BH Group, 25.00 g/kg), with 20 animals in each group. A rat rubrospinal tract (RST) transection model was established and treated for 28 days. The recovery of motor function of rats was observed through inclined plate test and spontaneous upright exploratory behavior test. Nissl's staining was used to observe the cell morphology of injured red nucleus neurons. Reverse Transcription PCR (RT-PCR) and immunofluorescence were used to detect the expression of ATG5 and Beclin1 mRNA. The Western blot method was used to observe the expression levels of Synaptophysin (SYP), Synaptosomal-associated Protein of 25 kDa (SNAP-25), Postsynaptic density protein 95 (PSD-95), ATG5, and Beclin1 proteins in red nucleus tissue. RESULTS: Compared with the SCI group, both BL and BH groups significantly improved the forelimb motor function and improved the status of red nucleus neurons in SCI rats. BYHWD increased SYP, SNAP-25, PSD-95, and decreased the red core Beclin1 and ATG5. CONCLUSIONS: BYHWD enhances synaptic regeneration and limb activity in red nucleus neurons of SCI rats by inhibiting autophagy.
摘要：
The present study aimed to explore the role of mitochondria‑associated membranes (MAMs) as a key interface between mitochondria and the endoplasmic reticulum (ER) and to evaluate their importance in maintaining the physiological functions of these two organelles. MAMs not only act as a structural bridge between mitochondria and the ER but also widely participate in the regulation of mitochondrial biosynthesis and function, Ca(2+) signal transduction, lipid metabolism, oxidative stress response and autophagy. In addition, the specific protein composition of MAMs is increasingly being recognized as having a profound impact on their function, and these proteins play a central role in regulating intercellular communication. Recently, the scientific community has accumulated a large amount of evidence supporting MAMs as potential targets for cardiovascular disease treatment. The present review focuses on the fine structure and multifunctional properties of MAMs and their mechanisms in the occurrence and development of cardiovascular diseases. The goal is to explore the mechanism of MAMs, therapeutic intervention points directly related to cardiovascular diseases, and feasibility of incorporating MAMs into the diagnostic strategy and treatment plan of cardiovascular diseases to provide novel insights and theoretical support for clinical practice in this field. MAMs have great potential as therapeutic targets for various cardiovascular diseases. This finding not only deepens the understanding of the interaction between organelles but also opens up a promising research path for the development of new therapeutic strategies for cardiovascular diseases.
摘要：
Diabetes is a metabolic disorder primarily characterized by persistent hyperglycemia. Diabetesinduced inflammation significantly compromises cardiovascular health, greatly increasing the risk of atherosclerosis. The increasing prevalence of harmful lifestyle habits and overconsumption has contributed substantially to the global rise in diabetes-related cardiovascular diseases, creating a significant economic and healthcare burden. Although current therapeutic strategies focus on blood glucose control and metabolic regulation, clinical observations show that diabetic patients still face persistent residual risk of AS even after achieving metabolic stability. Recent studies suggest that this phenomenon is linked to diabetes-induced trained immunity. Diabetes can induce trained immunity in bone marrow progenitor cells and myeloid cells, thus promoting the long-term development of AS. This article first introduces the concept and molecular mechanisms of trained immunity, with particular emphasis on metabolic and epigenetic reprogramming, which plays a crucial role in sustaining chronic inflammation during trained immunity. Next, it summarizes the involvement of trained immunity in diabetes and its contribution to AS, outlining the cell types that can be trained in AS. Finally, it discusses the connection between diabetes-induced trained immunity and AS, as well as the potential of targeting trained immunity as an intervention strategy. Understanding the molecular mechanisms of trained immunity and their impact on t disease progression may provide innovative strategies to address the persistent clinical challenges in managing diabetes and its complications.
作者机构：
[Zhou, Shunhua; Zhong, Meiqi; Xiong, Meng; Gao, Qing; Xie, Mengzhou; Yu, Chang; Peng, Qinghua; Ren, Baoping; Wang, Xiaojuan; Song, Houpan] Hunan Provincial Key Laboratory of Traditional Chinese Medicine Diagnostics, Hunan University of Chinese Medicine, Changsha, Hunan Province, China;[Zhou, Shunhua; Zhong, Meiqi; Xiong, Meng; Gao, Qing; Xie, Mengzhou; Yu, Chang; Peng, Qinghua; Ren, Baoping; Zeng, Meiyan; Wang, Xiaojuan; Song, Houpan] College of Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha, Hunan Province, China;[Zhou, Shunhua; Zhong, Meiqi; Xiong, Meng; Gao, Qing; Xie, Mengzhou; Yu, Chang; Peng, Qinghua; Ren, Baoping; Wang, Xiaojuan; Song, Houpan] Key Laboratory of TCM Heart and Lung Syndrome Differentiation & Medicated Diet and Dietotherapy, Hunan University of Chinese Medicine, Changsha, Hunan Province, China
摘要：
BACKGROUND: The study aimed to conduct a network meta-analysis of randomized controlled trials (RCTs) to examine the effectiveness and safety of traditional Chinese patent medicine (TCPM), either used alone or combined with conventional treatment (CT) of chemical drugs, for treating chronic atrophic gastritis (CAG). METHODS: We searched the literature from database creation to December 2023; our primary endpoint was clinical response rate. Secondary outcome measures were the inhibition rate of Helicobacter pylori and clinical symptom score, including pain and noisy scores for the stomach, score for belch and acid reflux, score for the bitter taste and the dry throat, and safety according to total adverse events. Data analyzed using RevMan 5.3, R (v4.0.2), and Stata SE 15.0. Mean differences (MDs) for continuous outcomes and odds ratios (ORs) for dichotomous outcomes and drug ranking by P-score. Network meta-analysis presented as forest plot and league table. Results reported as MDs/ORs with 95% confidence interval (CI). RESULTS: Our analysis comprised 49 RCTs with 5218 patients. Compared to using CT alone, all TCPMs + CT performed better in improving clinical response rate. The Weifuchun tablet (WFCT) inhibition rate (OR = 9.05; 95% CI = 1.89-43.34; P-score = .92) was relatively high, but only 1 RCT supported this result. WFCT + CT (OR = 2.94; 95% CI = 1.97-4.39; P-score = .57), Qizhiweitong granule (QZWTG) + CT (OR = 2.91; 95% CI = 1.07-7.89; P-score = .55), and Moluodan (MLD) + CT (OR = 2.44; 95% CI = 1.37-4.36; P-score = .43) had certain advantages in inhibiting H pylori compared to using CT alone. Compared with CT, Weisu tablet (WST) + CT (OR = 0.21; 95% CI = 0.05-0.89; P-score = .24) and Xiangshayangwei pill (XSYWP) + CT (OR = 0.41; 95% CI = 0.18-0.92; P-score = .44) were drugs that were less likely to cause adverse reactions. CONCLUSION: Our results demonstrate that, compared to using CT alone, TCPMs combined with CT can improve the clinical response rate in the treatment of CAG. Additionally, some TCPMs combined with CT can enhance the H pylori inhibition rate. WST + CT and XSYWP + CT can even reduce the occurrence of adverse reactions.
期刊：
International Journal of Biological Macromolecules,2025年304(Pt 1):140763 ISSN：0141-8130
通讯作者：
Shen, L;Chen, W
作者机构：
[Li, Jinxia] Hunan Univ Chinese Med, Changsha 410208, Peoples R China.;[Li, Jinxia] Hunan Univ Chinese Med, Prov Key Lab TCM Diagnost, Changsha 410208, Peoples R China.;[Shen, Li; Ning, Zixin; Yang, Xiaoyu; Gao, Kun] China Acad Chinese Med Sci, Inst Basic Theory TCM, Beijing 100700, Peoples R China.;[Liu, Ying] China Acad Chinese Med Sci, Inst Chinese Mat Med, Beijing 100700, Peoples R China.;[Chen, Wei; Chen, W] Zhejiang Univ, Sir Run Run Shaw Hosp, Sch Med, Dept Gen Surg, Hangzhou 310016, Peoples R China.
通讯机构：
[Shen, L ] C;[Chen, W ] Z;China Acad Chinese Med Sci, Inst Basic Theory TCM, Beijing 100700, Peoples R China.;Zhejiang Univ, Sir Run Run Shaw Hosp, Sch Med, Dept Gen Surg, Hangzhou 310016, Peoples R China.
关键词：
Actinidia eriantha polysaccharide;Macrophage;Gastric cancer
摘要：
Actinidia eriantha polysaccharide (AEPS), an active component of the Chinese herbal medicine Radix Actinidia chinensis , has anticancer effects. Here, we investigated the therapeutic potential of AEPS in gastric cancer through in vivo, in vitro, and in silico analyses. In our gastric cancer xenograft mouse model, AEPS effectively reduced tumor volume and weight. In the human gastric adenocarcinoma cell line AGS, AEPS inhibited migration and invasion without affecting proliferation; this result was validated in a fluorescent-labeled lung metastasis mouse model. Mass cytometry revealed that AEPS enhanced macrophage proportions in gastric cancer tissues. Bioinformatics analysis revealed a strong increase in PD-1-regulated M2 macrophage proportions in patients with gastric cancer, shortening their survival time and worsening their prognosis. In the coculture system of interleukin-4-induced human monocytic leukemia cells and AGS cells, AEPS treatment downregulated PD-1/PD-L1 and M2 macrophage–related marker expression but promoted TAM polarization toward the M1 phenotype. These findings facilitated the elucidation of the mechanism underlying the treatment effects of AEPS against gastric cancer. Finally, AEPS–PD-1 antibody combination therapy further enhanced the anticancer effects of AEPS in vivo, suggesting its potential as a basis to develop novel therapeutics for gastric cancer.
Actinidia eriantha polysaccharide (AEPS), an active component of the Chinese herbal medicine Radix Actinidia chinensis , has anticancer effects. Here, we investigated the therapeutic potential of AEPS in gastric cancer through in vivo, in vitro, and in silico analyses. In our gastric cancer xenograft mouse model, AEPS effectively reduced tumor volume and weight. In the human gastric adenocarcinoma cell line AGS, AEPS inhibited migration and invasion without affecting proliferation; this result was validated in a fluorescent-labeled lung metastasis mouse model. Mass cytometry revealed that AEPS enhanced macrophage proportions in gastric cancer tissues. Bioinformatics analysis revealed a strong increase in PD-1-regulated M2 macrophage proportions in patients with gastric cancer, shortening their survival time and worsening their prognosis. In the coculture system of interleukin-4-induced human monocytic leukemia cells and AGS cells, AEPS treatment downregulated PD-1/PD-L1 and M2 macrophage–related marker expression but promoted TAM polarization toward the M1 phenotype. These findings facilitated the elucidation of the mechanism underlying the treatment effects of AEPS against gastric cancer. Finally, AEPS–PD-1 antibody combination therapy further enhanced the anticancer effects of AEPS in vivo, suggesting its potential as a basis to develop novel therapeutics for gastric cancer.
期刊：
JOURNAL OF CRANIOFACIAL SURGERY,2025年 ISSN：1049-2275
作者机构：
[Mo, Qiulian; Yang, Meijun; Li, Chunhui] Department of Neurosurgery, The First Hospital of Hunan University of Chinese Medicine, Changsha, Hunan, People's Republic of China;[Wu, Yingjie; Liang, Hao] Hunan Provincial Key Laboratory of TCM Diagnostics, Institute of TCM Diagnostics, Hunan University of Chinese Medicine, Changsha, Hunan, People's Republic of China
摘要：
Dural closure is a crucial step in cranial surgery, essential for preventing complications like cerebrospinal fluid leakage, wound infections, and meningitis. Traditional suturing techniques, however, pose challenges such as technical difficulty and the potential for tissue damage. This retrospective study aimed to assess the safety and effectiveness of a nonsuture dural closure method using medical glue for direct adhesion of a patch to the dura mater. It was conducted from September 2019 to September 2023, including 169 patients with supratentorial brain injuries who underwent nonsuture dural closure (glue group) and 209 patients who received suture dural closure (suture group). The study compared the operation time and material costs between the two groups, with patients followed for 3 months to monitor complications and adverse events. The results showed that the operation time for dural closure was significantly shorter in the glue group (8.4 ± 3.7 min) compared with the suture group (21.7 ± 4.1 min). Postoperative complications were significantly lower in the glue group. There were no significant differences in adverse events between the groups. These findings suggest that nonsuture dural closure with medical glue is a simple, efficient, and safe alternative to traditional suturing, effectively reducing postoperative complications.
摘要：
BACKGROUND: Tongxinluo capsule, a formally classical commercial Chinese polyherbal preparation, has been utilized to treat patients with acute myocardial infarction for decades. PURPOSE: This meta-analysis aimed to comprehensively evaluate the clinical outcomes of tongxinluo capsule treated acute myocardial infarction. METHODS: Randomized controlled trials evaluating the effectiveness of tongxinluo capsule alone or in combination with conventional therapy in patients with acute myocardial infarction were identified from eight major databases: Chinese Biomedical Medicine, China National Knowledge Infrastructure, Wanfang Med Database, China Science and Technology Journal Database, PubMed, and Cochrane Central Register of Controlled Trials. In addition, two clinical trial registry platforms (clinicalTrials.gov and the WHO International Clinical Trials) were also searched for relevant studies, with the search extending to all published literature until December 2024. The initial screening and evaluation of the studies were carried out by two independent reviewers who assessed each study according to predefined eligibility criteria. The risk of bias in the research was evaluated using the Cochrane Collaboration's methodology for assessing methodology. Meta-analysis was carried out using RevMan 5.3 software, and publication bias was assessed utilizing StataMP 14.0. The evidence's quality was determined by the Grading of Recommendations Assessment, Development, and Evaluation process. RESULTS: This research included a total of 36 randomized controlled trials with 7002 patients. The meta-analysis revealed that Tongxinluo capsule combined with conventional treatment significantly decreased the 1-month MACCE rate (RR = 0.62, 95% CI 0.47 to 0.81; p = 0.0007), along with the individual risks of 1-month MACCE, including cardiac death (RR = 0.68, 95% CI 0.50 to 0.93; p = 0.02) and myocardial reinfarction (RR = 0.11, 95% CI 0.01 to 0.94; p = 0.04). After 12months of treatment, the MACCE rate (RR = 0.61, 95% CI 0.49 to 0.75; p < 0.00001), cardiac death (RR = 0.69, 95% CI 0.50 to 0.96; p = 0.03), myocardial reinfarction (RR = 0.32, 95% CI 0.13 to 0.75; p = 0.009), and stroke (RR = 0.42, 95% CI 0.20 to 0.87; p = 0.02) were also reduced. The remaining secondary outcomes-1-month stroke (RR = 0.44, 95% CI 0.44 to 1.44; p = 0.18), 12-month (RR = 0.12, 95% CI 0.01 to 2.14; p = 0.15) emergent coronary revascularization, 12-month all-cause mortality (RR = 0.78, 95% CI 0.60 to 1.01; p = 0.06)-showed no differences. Furthermore, the combination of Tongxinluo capsule and conventional therapy increased the incidence of the adverse drug reaction, mainly gastrointestinal discomfort (RR = 1.80, 95% CI 1.14 to 2.84; p = 0.01). However, there were no differences in the liver function levels of aspartate transaminase (SMD = -0.24, 95% CI -0.54 to -0.07; p = 0.12) and alanine aminotransferase (SMD = -0.25, 95% CI -0.55 to 0.05; p = 0.11), or the kidney function levels of blood urea nitrogen (SMD = 0.32, 95% CI -0.21 to 0.86; p = 0.23) and creatinine (SMD = 0.10, 95% CI -0.20 to 0.40; p = 0.52). CONCLUSION: Current data indicates that Tongxinluo capsule, used as an adjuvant treatment, may enhance clinical outcomes for AMI patients at 1- and 12-month. Moreover, it may enhance heart function, regulate lipid peroxidation, and suppress inflammatory levels.
摘要：
BACKGROUND: The interaction mechanisms between obesity and psychological factors are intricate and bidirectional. Psychological issues can prompt unhealthy eating behaviors, impede weight management efforts, and elevate the risk of obesity. This study employs bibliometric approaches to conduct a comprehensive analysis of the knowledge structure, research hotspots, and development trends in the field of obesity and psychology, offering valuable references for future research in this area. METHODS: This study draws on the Web of Science Core Collection (WoSCC) database, with "obesity" and "psychology" serving as the primary search terms. Leveraging CiteSpace (version 6.3.R1) and VOSviewer (version 1.6.20) software, bibliometric analyses were conducted on various indicators, including the number of publications, publication volume, authors, journals, references, countries, institutions, and keywords. Through co-citation analysis and keyword co-occurrence analysis, the research hotspots and developmental trajectories in this field were revealed. RESULTS: Based on the inclusion and exclusion criteria, a total of 2,753 relevant articles were ultimately included in this study. The results indicate that since the 21st century, there has been a significant surge in the number of publications in the field of obesity and psychology. Developed countries like the United States, Canada, the United Kingdom, and Australia are at the forefront of this field. Leading research institutions include Yale University, University College London, and the University of Pennsylvania. Among the authors, GRILO CM has the highest publication output. Research hotspot keywords primarily include "depression," "stress," "emotional eating," "bariatric surgery," "intervention," "weight stigma," and "self-regulation." Current research trends reveal a marked regional imbalance in international collaboration in the field of obesity and psychology. In particular, there exists a notable absence of substantive cooperation between developed and developing countries. Research hotspots mainly center around the following aspects: Firstly, it focuses on the prevalence of common psychological distress symptoms, including depression, anxiety, and stress, within the obese population and the implications these symptoms have for health. Secondly, mental health issues like binge eating and emotional eating play a pivotal role in the onset and maintenance of obesity. Thirdly, psychosocial factors like health-related quality of life and weight stigma are at the core of obesity intervention and have potential impacts on behavioral change. Meanwhile, researchers are increasingly concentrating on the individualized mental health requirements of obese populations, emphasizing the importance of evidence-based psychological interventions in the management of obesity. These research hotspots not only enhance our understanding of the complex relationship between obesity and mental health but also provide crucial theoretical foundations and practical insights for future research directions. CONCLUSION: This study employs bibliometric approaches to conduct a comprehensive and in-depth analysis of research trends and developments in the field of obesity and psychology. The research reveals the current status and characteristics of this field from multiple perspectives, offering scientific backing for researchers to identify potential collaborators, pinpoint hotspot issues, and keep abreast of the latest developments. Looking forward to the future, related research can further expand data sources, diversify research viewpoints, and delve more profoundly into the complex relationship between obesity and mental health.
作者机构：
[Li, Liang; Xia, Shuai-shuai; Li, Mei-li; Fan, Ting-ting; Wan, Cai-yun; Li, Si-yuan] Hunan Univ Chinese Med, Prov Key Lab Tradit Chinese Med Diagnost, Changsha 410208, Peoples R China.;[Li, Liang; Xia, Shuai-shuai; Li, Mei-li; Fan, Ting-ting; Wan, Cai-yun; Li, Si-yuan] Hunan Univ Chinese Med, Key Lab TCM Heart & Lung Syndrome Differentiat & M, Changsha 410208, Peoples R China.;[Yin, Jian; Li, Mei-li] Hunan Univ Chinese Med, Dept Anat, Changsha 410208, Peoples R China.;[Li, Qiang] Hunan Brain Hosp, Dept Tradit Chinese Med, Changsha 410007, Peoples R China.
通讯机构：
[Li, L ] H;Hunan Univ Chinese Med, Prov Key Lab Tradit Chinese Med Diagnost, Changsha 410208, Peoples R China.;Hunan Univ Chinese Med, Key Lab TCM Heart & Lung Syndrome Differentiat & M, Changsha 410208, Peoples R China.
摘要：
OBJECTIVE: To investigate whether Buyang Huanwu Decoction (BYHWD) had a good curative effect on the neuroprotection of red nucleus neurons after spinal cord injury (SCI) and the possible molecular mechanism. METHODS: Ninety male Sprague-Dawley rats were divided into 5 groups (n=18 per group) according to a random number table, including the control, model, low- (12.78 g/kg, BL group), medium- (25.65 g/kg, BM group), and high-dose BYHWD groups (51.30 g/kg, BH group). A rubrospinal tract transection model in rats was established, and different doses of BYHWD were intragastrically administrated for 4 weeks. The forelimb locomotor function was recorded using the spontaneous vertical exploration test. Cyclic adenosine monophosphate (cAMP) level in red nucleus was detected through an enzyme-linked immunosorbent assay. The morphology and number of red nucleus neurons were observed using Nissl's staining and axonal retrograde tracing by Fluoro-Gold (FG). The expression of cAMP-dependent protein kinase A (PKA), nuclear factor kappa-B (NF-κB) p65, and brain-derived neurotrophic factor (BDNF) in red nucleus were detected using immunohistochemistry and quantitative real-time polymerase chain reaction. RESULTS: Compared with the control group, the utilization rate of bilateral forelimbs, unilateral right forelimbs, proportion of FG-labeled positive neurons, cAMP level, protein expressions of PKA and BDNF, and BDNF mRNA expression were significantly decreased in the model group (P<0.01), while NF-κB p65 was increased in the model group (P<0.01). Compared with the model group, the utilization rate of bilateral forelimbs and unilateral right forelimbs were significantly higher in the BL, BM and BH groups (P<0.01), the proportion of FG-labeled positive neurons, cAMP level, protein expressions of PKA and BDNF and BDNF mRNA expression in all BYHWD groups were increased (P<0.05 or P<0.01), while NF-κB p65 were decreased in all BYHWD groups (P<0.05 or P<0.01). CONCLUSIONS: BYHWD possesses a sound neuroprotective effect on red nucleus neurons after SCI, and the efficacy was dose-related. The mechanism may be related to regulating the cAMP/PKA/NF-κ B p65 signaling pathway, finally promoting expression of BDNF.
摘要：
BACKGROUND: Diuretic resistance (DR) is characterized by insufficient fluid and sodium excretion enhancement despite maximum loop diuretic doses, indicating a phenotype of refractory heart failure (HF). Recently, metabolomics has emerged as a crucial tool for diagnosing and understanding the pathogenesis of various diseases. This study aimed to differentiate diuretic-resistant patients from non-resistant HF to identify biomarkers linked to the emergence of DR. METHODS: Serum samples from HF patients, both with and without DR, were subjected to non-targeted metabolomic analysis using liquid chromatography-tandem mass spectrometry. Metabolite variations between groups were identified using principal component analysis and orthogonal partial least-square discriminant analysis. Metabolic pathways were assessed through the Kyoto Encyclopedia of Genes and Genomes database enrichment analysis, and potential biomarkers were determined using receiver operating characteristic curves (ROCs). RESULTS: In total, 192 metabolites exhibited significant differences across the two sample groups. Among these, up-regulation was observed in 164 metabolites, while 28 metabolites were down-regulated. A total of 28 pathways involving neuroactive ligand-receptor interaction and amino acid biosynthesis were affected. The top five metabolites identified by ROC analysis as potential DR biomarkers were hydroxykynurenine, perillic acid, adrenic acid, 5-acetamidovalerate, and adipic acid. CONCLUSIONS: Significant differences in metabolite profiles were observed between the diuretic-resistant and non-diuretic-resistant groups among patients with HF. The top five differentially expressed endogenous metabolites were hydroxykynurenine, perillic acid, adrenic acid, 5-acetamidovalerate, and adipic acid. The metabolic primary pathways implicated in DR were noted as amino acid, energy, and nucleotide metabolism. CLINICAL TRIAL REGISTRATION: This study was registered with the China Clinical Trials Registry (https://www.chictr.org.cn/hvshowproject.html?id=197183&v=1.7, ChiCTR2100053587).
摘要：
BACKGROUND: Lung cancer is a highly prevalent neoplastic disease in various regions of the world, but the mechanism of its occurrence, development, and metastasis is not clear. Different hormone levels have different potential roles in the occurrence, development, and metastasis of lung cancer, but the association between hormone levels and lung cancer is not clear. OBJECTIVE: This study aims to explore the causal relationship between hormone levels and lung cancer using Mendelian randomization. Sensitivity and heterogeneity tests were conducted to ensure the reliability of the results, offering insights into the prevention, diagnosis, and treatment of lung cancer. METHODS: We employed a two-sample Mendelian randomization (MR) analysis using large-scale publicly available genome-wide association studies (GWAS) data to assess the causal relationship between hormone levels and lung cancer. We explored the causal relationship between 15 hormones and three subtypes of lung cancer. The inverse variance weighted (IVW) method was used as the primary analysis, while MR-Egger, weighted median, weighted mode, and simple median were applied as supplementary methods. Sensitivity and heterogeneity tests were conducted to ensure the robustness of the findings. RESULTS: We identified six hormone levels to be significantly associated with lung squamous cell carcinoma (LUSC): total testosterone, oestradiol, thyrotropin-releasing hormone, insulin, parathyroid hormone, and glucocorticoid. Among them, total testosterone, estradiol, and thyrotropin-releasing hormone were negatively correlated with morbidity. Insulin, prolactin levels, and parathyroid hormone were positively correlated with morbidity. Five hormone levels were significantly associated with lung adenocarcinoma (LUAD): luteinizing hormone, thyroid hormones, insulin, prolactin levels, and parathyroid hormone. Luteinizing hormone and thyroid hormones were negatively correlated with morbidity, while insulin, prolactin levels, and parathyroid hormone were positively correlated with morbidity. Similarly, five hormone levels were linked to small cell lung cancer (SCLC): total testosterone, luteinizing hormone, estradiol, PTHrP, and insulin. Total testosterone and luteinizing hormone were negatively correlated with morbidity, while estradiol, Parathyroid Hormone-Related Peptide (PTHrP), and insulin were positively correlated with morbidity. Several hormones were associated with different subtypes of lung cancer. Insulin was significantly associated with all three types of lung cancer. Testosterone showed positive effects in LUSC and SCLC, and estradiol had varying effects, with a negative correlation in SCLC and a positive correlation in LUSC. Testosterone and estradiol were not significantly associated with LUAD. Luteinizing hormone showed positive effects in LUAD and SCLC, and parathyroid hormone showed negative effects in LUSC and LUAD. CONCLUSION: This study demonstrates significant causal relationships between specific hormone levels and various types of lung cancer, providing valuable insights for prevention, diagnosis, and treatment strategies of lung cancer.
期刊：
International Journal of Biological Macromolecules,2025年297:139864 ISSN：0141-8130
通讯作者：
Qian, Chuiwen;Liu, ZH;Lin, JS
作者机构：
[Cui, Xin; Qian, Chuiwen; Li, Zhentao; Liu, Zonghua; Qian, CW; Yue, Yilin; Shi, Xinyu] Jinan Univ, Coll Life Sci & Technol, Guangzhou 510632, Peoples R China.;[Lin, Jiansheng] Hunan Univ Chinese Med, Dept Anat, Changsha 410208, Peoples R China.
通讯机构：
[Lin, JS ] H;[Qian, CW; Liu, ZH ] J;Jinan Univ, Coll Life Sci & Technol, Guangzhou 510632, Peoples R China.;Hunan Univ Chinese Med, Dept Anat, Changsha 410208, Peoples R China.
关键词：
Diabetic wound;Hyaluronic acid;Hydrogel;Phytic acid;Recombinant human Amelogenin
摘要：
Diabetic wounds present a considerable challenge in modern medicine due to their prolonged healing process, driven by sustained inflammation and impaired vascular regeneration. This study introduces a novel hydrogel network through osmosis, utilizing hyaluronic acid (HA) and phytic acid (PA) for their anti-inflammatory and antioxidant properties, respectively. By incorporating recombinant Human Amelogenin (rhAM), known for its angiogenic potential, we aimed to develop the HA-PA-rhAM hydrogel to enhance wound healing in diabetic rats. Our results indicate that this hydrogel has excellent mechanical properties, stability, electrical conductivity, and effective adaptation to irregular wound shapes. In vitro cellular assays demonstrated the hydrogel has an excellent biosafety profile, pro-cell migration, and pro-angiogenic capacity, and the diabetic rat full-thickness wound model further indicated the hydrogel's capacity to promote wound repair, by down-regulating inflammatory factors, promoting the transition of M1-type macrophages to M2-type, and up-regulating the expression of angiogenic markers. In summary, this functional hydrogel has a simple and efficient synthetic pathway and easy clinical translation, making it highly promising for treating chronic diabetic wounds.
摘要：
This study aimed to explore the potential of using mesenchymal stem cell (MSC)-derived exosomes (MSC-Exos) pre-treated with Astragaloside IV (ASIV) to alleviate inflammation in high glucose (HG)-damaged endothelial cells. MSC-Exos were isolated from untreated MSCs and ASIV-pre-treated MSCs, and their characteristics were assessed. The expression of miR-146a-5p in MSC-Exos was determined, and it was found that ASIV treatment enhanced its expression. In order to assess the impact of highly miR-146a-5p-expressing MSC-Exos on HG-injured endothelial cells, we established a model of HG-induced inflammation using human umbilical vein endothelial cells (HUVECs). The study measured cell viability, apoptosis, tube formation, and levels of inflammatory cytokines among the different treatment groups. It was found that transferring MSC-Exos with high miR-146a-5p expression to HG-damaged HUVECs increased cell viability and tube formation ability while reducing the number of apoptotic cells. Additionally, changes in inflammatory factors indicated a reduction in the inflammatory response. Further investigation demonstrated that miR-146a-5p inhibited the expression of TNF receptor associated factor 6 (TRAF6) and phosphorylated NF-κB, which are involved in the inflammatory response. This resulted in the alleviation of inflammation in HG-damaged endothelial cells. In summary, our findings indicate that ASIV treatment stimulated the secretion of MSC-Exos that exhibited increased levels of miR-146a-5p. These exosomes, in turn, regulated the TRAF6/NF-κB pathway. As a result of this modulation, the inflammatory response in HG-damaged endothelial cells was alleviated. These findings offer a fresh approach to addressing vascular complications associated with diabetes, which could lead to novel treatment strategies in the field.
摘要：
Epimedium has long been used as an ethnic drug in Asia and Europe for its high medicinal value and health benefits, which is often used for anti-tumor, anti-osteoporosis, sexual dysfunction, and other related diseases. In this research, Epimedium is reviewed in the botany, phytochemistry, pharmacology, toxicology, processing, and quality control to make it better for clinical services. More than 106 compounds, including flavonoids, polysaccharides, alkaloids, lignans, and others, were isolated from Epimedium . Based on multiple chemically active components, Epimedium has a wide range of pharmacological action. Several studies have demonstrated Epimedium has multiple biological activities, including neuroprotective effects, anti-inflammatory, anti-aging, and antioxidant effects, anti-osteoporosis, anti-cancer, anti-tumor, anti-diabetes, anti-influenza, effects on sexual dysfunction, etc. Recently, the toxicity of Epimedium has been brought into focus, and its hepatotoxicity has been confirmed through animal experiments. Moreover, it is particularly important to control its quality. To date, great progress has been made in the study of Epimedium . This study systematically reviews the achievements of research on Epimedium . Moreover, the shortcomings of the current research on Epimedium were pointed out, and some suggestions were given.
摘要：
OBJECTIVE: To compare three common stimuli that induce emotional stress to identify the optimal method for establishing an animal model that aligns with the clinical pathogenesis of irritable bowel syndrome (IBS) and to explore the gut microbiota mechanisms underlying IBS development. METHODS: Thirty-six SPF-grade female Kunming mice were randomly divided into four groups: the normal control (NC) group, the restraint stress (BM) group, the tail clamp stress (CTM) group, and the restraint combined with tail clamp stress (BCTM) group, with 9 mice in each group. The NC group was fed normally without any stimulation. The BM group was subjected to restraint stress. The CTM group received intermittent tail clamp stress. The BCTM group underwent both restraint stress and intermittent tail clamp stress. The stimulation time for each group was 1 hour, and the modeling duration was 7 days. General behavioral changes in the mice were observed. The fecal water content was measured and calculated. The pain threshold, gastric residue rate, small intestine propulsion rate, and serum levels of short-chain fatty acids (SCFAs), serotonin (5-HT), interleukin-10 (IL-10), and tumor necrosis factor-alpha (TNF-α) were assessed. Histopathological analysis of the small intestine and colon tissues was performed. 16S rRNA high-throughput sequencing was subsequently conducted. The effects of different stimuli on mouse symptoms, gastrointestinal motility, visceral hypersensitivity, inflammation levels, and the gut microbiota were analyzed, and correlation analysis was performed. RESULTS: Compared with the NC group, the BM, CTM, and BCTM groups of mice presented varying degrees of emotional hyperreactivity, accompanied by significantly reduced food intake and fecal water content and markedly elevated levels of inflammation, all of which are indicative of IBS symptoms. Among them, the BCTM group presented the most pronounced emotional hyperreactivity and irritability. The mice in the BCTM group had significantly higher gastric residue rates and 5-HT levels, with a marked reduction in pain tolerance. The gut microbiota of the mice in the BM, CTM, and BCTM groups all exhibited dysbiosis, with changes in the diversity, structural composition, and function of the microbial community. Specific bacterial taxa were enriched in each stress group, and their corresponding KEGG pathways were also significantly altered. Correlation analysis revealed that SCFAs were significantly positively correlated with the small intestine propulsion rate, whereas 5-HT was positively correlated with the gastric residue rate and negatively correlated with the pain threshold. SCFAs were positively correlated with IL-10 and TNF-α, and 5-HT was significantly positively correlated with IL-10 and TNF-α. In the BCTM group, the characteristic bacteria Acinetobacter and Akkermansia were significantly correlated with SCFAs and 5-HT. CONCLUSION: 1. The restraint combined with the tail clamp stress method is superior among the three stress protocols and successfully induces the IBS mouse model. 2. Acinetobacter and Akkermansia may contribute to the development of IBS induced by restraint combined with tail clamp stress through the regulation of SCFAs and 5-HT.
摘要：
Inadequate antigen capture and insufficient antigen-presenting cell (APC) activity at tumor sites limit the effectiveness of in situ vaccines. To address this, poly(glutamic acid-cholinephosphate) (pGluCP) is introduced as a polymer with cell membrane adhesion properties capable of capturing both water-soluble and insoluble membrane antigens from necrotic tumor cells while recruiting more APCs. The approach uses manganese-mineralized black phosphorus (MnBP) coated with pGluCP and αPD-1 antibodies to create the MnBP@pGluCP-αPD-1 complex for in situ vaccines. MnBP eradicates tumor cells via photothermal effects, releasing antigens, while Mn(2)⁺ ions activate the intracellular STING pathway, acting as an adjuvant. pGluCP captures these antigens, forming pathogen-mimicking micro-nanoparticles, leading to an in situ vaccine (MnBP@pGluCP/antigens) that co-localizes antigens and adjuvants. The αPD-1 antibody alleviates tumor-induced immune suppression, enhancing tumor cell-specific killing. This study demonstrates the potential of leveraging cholinephosphate-cell membrane interactions to improve antigen presentation efficiency, significantly bolstering the efficacy of in situ tumor vaccines and opening new avenues for advanced cancer immunotherapy.
作者机构：
[Chen, Hong-Yao; Wu, Ling; Xue, Pei-Sen; Wang, Zhi-Bin; Yang, Ren-Yi; Zhang, Jing-Ting; Gao, Wen-Hui] Hunan Univ Chinese Med, Coll Tradit Chinese Med, Changsha 410208, Hunan, Peoples R China.;[Peng, Wei; Li, Ke-Xiong] Hunan Prov Hosp Integrated Tradit Chinese & Wester, Dept Oncol, Changsha 410006, Hunan, Peoples R China.;[Zeng, Pu-Hua] Hunan Acad Tradit Chinese Med, Hunan Prov Hosp Integrated Tradit Chinese & Wester, Canc Res Inst, 58 Lushan Rd, Changsha 410006, Hunan, Peoples R China.
通讯机构：
[Zeng, PH ] H;Hunan Acad Tradit Chinese Med, Hunan Prov Hosp Integrated Tradit Chinese & Wester, Canc Res Inst, 58 Lushan Rd, Changsha 410006, Hunan, Peoples R China.
关键词：
Bioinformatics;Chlorogenic acid;Ferroptosis;Hepatocellular carcinoma;In vitro experiment;Prostaglandin endoperoxide synthase 2/aldo-keto reductase family 1 member C3/glutathione peroxidase 4 axis
摘要：
BACKGROUND: Ferroptosis is an iron-dependent programmed non-apoptotic cell death characterized by the accumulation of free iron ions and lipid peroxidation. It is associated with the inactivation of glutathione peroxidase (GPX) and the accumulation of lipid peroxides within cells. Ferroptosis is closely related to the occurrence and development of hepatocellular carcinoma (HCC). Chlorogenic acid (CGA), an important bioactive component found in 61 traditional Chinese medicines such as Eucommia ulmoides, has been extensively studied for its effects on various malignant tumors. However, the specific role and potential mechanism of CGA in HCC remain unclear. AIM: To elucidate the anti-tumor characteristics and potential mechanisms of CGA in inducing ferroptosis in HCC cells. METHODS: The effects of CGA on the proliferation, migration, and invasion of HCC cells were evaluated through in vitro experiments. Bioinformatics analysis combined with network pharmacology was used to study the potential targets and molecular mechanisms of CGA intervention in HCC ferroptosis. In vitro experiments were conducted to verify and explore the anti-HCC effects and mechanisms of CGA through the ferroptosis pathway. RESULTS: In vitro experiments showed that CGA dose-dependently inhibited the proliferation, invasion, and migration of HCC cells. Bioinformatics analysis combined with network pharmacology revealed that the pathway of CGA intervention in HCC cell ferroptosis was mainly enriched in the prostaglandin endoperoxide synthase 2 (PTGS2)/aldo-keto reductase family 1 member C3 (AKR1C3)/GPX4 signaling pathway, which was associated with arachidonic acid. In vitro experiments further confirmed that CGA-induced ferroptosis in HCC cells was related to mitochondrial damage through the reprogramming of arachidonic acid metabolism by regulating the PTGS2/AKR1C3/GPX4 signaling pathway. CONCLUSION: This study demonstrates that CGA inhibits HCC cell proliferation, migration, and invasion by inducing ferroptosis through the PTGS2/AKR1C3/GPX4 axis, suggesting its potential as a novel ferroptosis inducer or anti-HCC drug.
摘要：
OBJECTIVE: To investigate the relationship between heart failure (HF) and gut microbiota-mediated energy metabolism, and to explore the role of Shenfu Injection in this process. MATERIALS AND METHODS: In this study, Adriamycin-induced chronic heart failure (CHF) rat model was used and randomly divided into the blank control group (Normal, n = 9), HF control group (Model, n = 12), Shenfu Injection treatment group (SFI, n = 9), and positive drug control group (TMZ, n = 9). The changes in gut microbiota structure were analyzed by 16S rRNA high-throughput sequencing, the content of short-chain fatty acids (SCFAs) was detected by targeted metabolomics technology, and cardiac function and energy metabolism-related indicators were evaluated. RESULTS: Myocardial energy metabolism in HF rats was disordered, characterized by reduced fatty acid oxidation, enhanced anaerobic glycolysis of glucose, mitochondrial damage, and decreased ATP content; The gut microbiota of HF rats was imbalanced, with a reduction in beneficial bacteria, an increase in conditional pathogenic bacteria, and impaired intestinal barrier function; Both Shenfu Injection and trimetazidine improved myocardial energy metabolism and cardiac function, but Shenfu Injection was more significant in regulating gut microbiota and improving intestinal health; The production of SCFAs from the gut microbiota of HF rats increased, which may be closely related to myocardial energy metabolism; SCFAs-producing bacteria Akkermansia and Blautia played a key role in the development of HF, and their abundance was positively correlated with SCFAs content. CONCLUSION: Shenfu Injection in treating HF may improve myocardial energy metabolism and intestinal health by regulating gut microbiota, especially the abundance of SCFAs-producing bacteria Akkermansia and Blautia, thereby exerting therapeutic effects. This provides theoretical support for treatment strategies based on gut microbiota.
