摘要：
Background: Recent studies have indicated that microRNA-15a (miR-15a) is dysregulated in breast cancer (BC). We aimed to evaluate the expression of miR-15a in BC tissues and corresponding para-carcinoma tissues. We also focused on effects of miR-15a on cellular behavior of MDA-MB-231 and expression of its target gene synuclein-${\gamma}$ (SNCG). Materials and Methods: The expression levels of miR-15a were analysed in BC formalin fixed paraffin embedded (FFPE) tissues by microarray and quantitative real-time PCR. CCK-8 assays, cell cycle and apoptosis assays were used to explore the potential functions of miR-15a in MDA-MB-231 human BC cells. A luciferase reporter assay confirmed direct targets. Results: Downregulation of miR-15a was detected in most primary BCs. Ectopic expression of miR-15a promoted proliferation and suppressed apoptosis in vivo. Further studies indicated that miR-15a may directly interact with the 3'-untranslated region (3'-UTR) of SNCG mRNA, downregulating its mRNA and protein expression levels. SNCG expression was negatively correlated with miR-15a expression. Conclusions: MiR-15a has a critical role in mediating cell cycle arrest and promoting cell apoptosis of BC, probably by directly targeting SNCG. Thus, it may be involved in development and progression of BC.
作者机构：
[贺福元] Department of Pharmaceutics, Hunan University of Chinese Medicine, Changsha 410208, China. pharmsharking@tom.com;[邓凯文] The First Affinity Hospital, Hunan University of Chinese Medicine, Changsha 410007, China;[石继连; 刘文龙; 黄胜] Department of Pharmaceutics, Hunan University of Chinese Medicine, Changsha 410208, China
关键词：
Apoptosis;Chemosensitivity;Lentivirus;Pancreatic cancer;Proliferation;RNA interference (RNAi);Survivin;X-linked apoptosis inhibiting protein (XIAP)
摘要：
At present, classic therapies provide limited benefits to the survival of patients with pancreatic cancer. However, clinically available gene therapy strategies have not been well established. This study investigates the effect of shRNA-mediated inhibition of XIAP and survivin expression on the proliferation, apoptosis, and chemosensitivity of pancreatic cancer cells. Stable inhibition of XIAP and survivin expression in SW1990 and Panc-1 pancreatic cancer cells was established by lentivirus-carried shRNAs. The mRNA and protein expression of XIAP and survivin were detected by real-time PCR and Western blot, respectively. Cell proliferation was measured by MTT assay, and apoptosis was detected by caspase-3/7 activity and Hoechst33342 staining. The lentivirus-carried shRNA significantly inhibited XIAP and survivin expression. Simultaneous inhibition of XIAP and survivin expression in pancreatic cells significantly reduced cell proliferation, increased caspase-3/7 activity, and increased cell sensitization to 5-FU and gemcitabine treatments compared to inhibition of XIAP or survivin expression alone. However, simultaneous silencing of XIAP and survivin showed no significant difference in inducing cell apoptosis compared to silencing of XIAP or survivin expression alone. Simultaneous inhibition of XIAP and survivin expression may be an effective strategy for gene therapy of pancreatic cancer.
通讯机构：
Property and Pharmacodynamic Key laboratory of Traditional Chinese Medicine M, State Administration of Chinese Medicine, Pharmaceutical Preparation Technology and Evaluation Laboratory of Traditional Chinese Medicine, China
作者机构：
[孙克伟; 王若宇] Liver Disease Center, First Hospital Affiliated to Hunan University of Traditional Chinese Medicine, Changsha (410007), China;[周宇帆] Center for Liver Transplant, Xiangya Hospital, Central Southern University, Changsha (410001), China;[费宇彤] Center for Evidence-Based Chinese Medicine, Beijing University of Chinese Medicine, Beijing (100029), China
摘要：
The objective of the study was to study the protective effect of Silybum marianum extract on hepatic ischemiareperfusion injury. Rats were randomly divided into five groups; namely Silybum marianum extract high-, medium-, and lowdose protection groups, model group and control group. Hepatic ischemia-reperfusion injury model was prepared. Serum or plasma AST, ALT, MDA, TNF-α, IL-1β, IL-6 levels were measured. The results revealed that after liver injury, AST, ALT, MDA, TNF-α, IL-1β, and IL-6 levels significantly increased in succession, showing significant differences. We concluded that inflammatory cytokines participate in liver injury and that Silybum marianum extract can reduce the production of inflammatory cytokines, and thus can have a protective effect on hepatic ischemia and reperfusion. Keywords : Silybum marianum , hepatic ischemia-reperfusion injury, protective effect
作者机构：
[Wu YiMou; Cao WenJuan; Zhou Zhou; Lu ChunXue; Zhou Hui; Li ZhongYu; Ma KangKang] Univ South China, Pathogen Biol Inst, Hengyang 421001, Peoples R China.;[Xie XiaoBing; Zhou Hui] Hunan Univ Chinese Med, Hosp 1, Dept Lab Med, Changsha 410007, Hunan, Peoples R China.;[Huang QiuLin] Univ South China, Affiliated Hosp 1, Dept Gen Surg, Hengyang 421001, Peoples R China.;[Zhong GuangMing] Univ Texas Hlth Sci Ctr San Antonio, Dept Microbiol & Immunol, San Antonio, TX 78229 USA.
通讯机构：
[Li ZhongYu] U;Univ South China, Pathogen Biol Inst, Hengyang 421001, Peoples R China.
关键词：
Chlamydia trachomatis;pORF5 plasmid protein;mitogen-activated protein kinase;proinflammatory cytokines;TLR2
摘要：
Infection with Chlamydia trachomatis induces inflammatory pathologies in the urogenital tract that can lead to infertility and ectopic pregnancy. Pathogenesis of infection has been mostly attributed to excessive cytokine production. However, precise mechanisms on how C. trachomatis triggers this production, and which protein(s) stimulate inflammatory cytokines remains unknown. In the present study, the C. trachomatis pORF5 protein induced tumor necrosis factor alpha (TNF-alpha), interleukin-1 beta (IL-1 beta) and interleukin-8 (IL-8) in dose- and time-dependent manners in the THP-1 human monocyte cell line. We found that intracellular p38/mitogen-activated protein kinase (MAPK) and extracellular signal-regulated kinase (ERK)/MAPK signaling pathways were required for the induction of TNF-alpha, IL-1 beta and IL-8. Blockade of toll-like receptor 2 (TLR2) signaling reduced induction levels of TNF-alpha, IL-8 and IL-1 beta. We concluded that the C. trachomatis pORF5 protein might contribute to the inflammatory processes associated with chlamydial infections.
通讯机构：
[Liu, Shaojun] C;Cent S Univ, Xiang Ya Sch Med, Affiliated Hosp 3, Dept Digest Med, Changsha 410078, Hunan, Peoples R China.
关键词：
Folium Cordylines Fruticosae Anti-gastric Cancer MGC-803 cell
摘要：
The active components in Folium Cordylines Fruticosae were extracted by heat reflux method. The solvents used were distilled water and ethanol. The effects of two types of extracts on gastric cancer cells were compared; dry extract yields were calculated, as well as the inhibition rates of gastric cancer MGC-803 cell proliferation and the colony cell counts. The micro-Kjeldahl method was used to measure the cell protein contents and to make a comprehensive comparison. The results showed that the MGC-803 cell inhibition rates of three different concentrations (32.5, 75 and 150 mg/ml) of ethanol extracts increased with the increase of concentration, which was 48.9% at a concentration of 150 mg/ml; aqueous extract of Folium Cordylines Fruticosae had very low inhibitory activity at a low concentration (32.5 mg/ml), which was remained at about 20%. After being affected by two types of extracts, cells had uneven sizes, with very low brightness, while the normal cells presented a uniform full form, with high definition.
摘要：
Objective：To investigate the regulatory effect of Jingang Jiangu pill （金刚健片, JGJG） on expression of inte- grin in ovariectomized rats. Methods：Fifty ovariectomized 10 months old female rats were randomly divided into 5 groups： Fushanmei group （FSM） ,Jingang Jiangu pill （ 金刚健片）group （JGJG） ,Gusongbao granule group （GSB） ,Model group （OVX）, Sham group. After ovariectomized,the rats were raised in the same environment for 13 weeks. The rats in JGJG group took 0.13 g JGJG pill orally each day for each rat;the rats in GSB group took 0.86 g GSB granule orally each day for each rat; the rats in FSM group took 0.28 mg FSM orally each day for each rat;and the rats in OVX and sham groups took sodium. The treatment duration of rats in above 5 groups was 13 weeks. Bone mineral density （BMD） and the expression of integrin β1 and αvβ3 were detected in each group after the treatment. Results：The BMD and the expression of integrin β1 in FSM group, JGJG group and GSB group improved obviously than that of OVX group. There were statistical difference between these groups （P〈0.05）. The expression of integrin αvβ3 of the three treating groups significantly depressed. Conclusion ：The JGJG pill im- proves BMD and express of integrin β1 in ovariectomized rats and reduces express of integrin αvβ3 through the regulation of the coupling of osteoblasts and osteoclasts.
作者机构：
[曹寅生] Department of Orthopaedics, the First Hospital Affiliated to Hunan University of Traditional Chinese Medicine, Changsha 410007, Hunan, China. caoyinsheng@126.com;[姚共和; 朱付平; 张波; 李卫宁; 卢敏] Department of Orthopaedics, the First Hospital Affiliated to Hunan University of Traditional Chinese Medicine, Changsha 410007, Hunan, China
