作者机构：
[Wang, Ting; Xiao, Xiao-Qin] Hunan Univ Chinese Med, Inst Combinat Chinese Tradit Med & Western Med, Changsha 410208, Hunan, Peoples R China.;[Liu, Jian] Hunan Univ Chinese Med, Hosp 1, Dept Emergency, Changsha 410208, Hunan, Peoples R China.;[Xiao, Xiao-Qin] Hunan Univ Chinese Med, Inst Combinat Chinese Tradit Med & Western Med, Bachelor Rd, Changsha 410208, Hunan, Peoples R China.
通讯机构：
[Xiao, Xiao-Qin] H;Hunan Univ Chinese Med, Inst Combinat Chinese Tradit Med & Western Med, Bachelor Rd, Changsha 410208, Hunan, Peoples R China.
摘要：
Cantharidin (CTD), a chemical compound secreted by blister beetles, has been shown with anti-tumor property in many cancer cells. In this study, our data showed that CTD exerts potent anti-angiogenesis activity in a dose-dependent manner. CTD dose dependently suppressed human umbilical vascular endothelial cells proliferation, migration, and tube formation in vitro. Furthermore, CTD concentration dependently inhibited angiogenesis in chick embryo CAM model in vivo. At the molecular level, CTD abrogated VEGF-induced activation of STAT3 and suppressed the phosphorylation of JAK1 and ERK in a dose-dependent manner. Furthermore, CTD blocked the phosphorylation of AKT in a time-dependent manner. Taken together, these findings clearly demonstrate for the first time that CTD can inhibit angiogenesis and may have applications in the development of new anti-angiogenesis drugs.
摘要：
Interleukin 35 (IL-35) is a newly discovered anti-inflammatory cytokine. Recent studies have indicated that it plays a crucial role in the pathogenesis of autoimmune diseases. In humans, IL-35 is predominantly secreted from regulatory T cells. This study aimed to measure serum IL-35 levels in patients with rheumatoid arthritis (RA) and in control individuals, and analyze its association with disease indicators of RA. One hundred patients with RA were recruited, and 50 volunteers were enrolled as healthy controls. Serum IL-35 levels were measured using an enzyme-linked immunosorbent assay kit. RA patients showed significantly lower serum levels of IL-35 compared with healthy controls (p < 0.001). RA patients suffering from erosive arthritis (n = 31) had lower IL-35 levels than those with non-erosive arthritis (n = 69, p = 0.022). In addition, serum IL-35 level was significantly lower in 22 patients with elevated percentage (> 75%) of neutrophils (p < 0.001). Correlation analysis indicated a significantly negative association between IL-35 and age, rheumatoid factor (RF), or percentage of neutrophils. In contrast, the serum IL-35 levels were not significantly different between patients with anti-cyclic citrullinated peptide (CCP) antibodies (n = 78) and those without anti-CCP antibodies (n = 22). However, among patients without anti-CCP antibodies, the serum IL-35 levels were lower in patients with erosive arthritis (n = 8) than those patients without erosion (n = 14) (p < 0.001), although no significant difference was detected in patients with anti-CCP antibodies. In conclusion, IL-35 plays a protective role in the pathogenesis of RA.
作者机构：
[Wu Xiaqiu; Peng Jin] China Acad Chinese Med Sci, Inst Basic Res Clin Med, Dept Prevent Med, Beijing 100700, Peoples R China.;[Li Guoqin] China Acad Chinese Med Sci, Guanganmen Hosp, Dept Resp, Beijing 100700, Peoples R China.;[Zhang Wei] Hunan Univ Chinese Med, Hosp 1, Dept Acupuncture & Massage, Changsha 410000, Hunan, Peoples R China.;[Liu Guangxia] Anhui Univ Chinese Med, Hosp 2, Dept Acupuncture, Hefei 230000, Peoples R China.;[Liu Baoyan] China Acad Chinese Med Sci, Beijing 100700, Peoples R China.
通讯机构：
[Peng Jin] C;China Acad Chinese Med Sci, Inst Basic Res Clin Med, Dept Prevent Med, Beijing 100700, Peoples R China.
关键词：
Acupoint sticking therapy;Quality of Life;Self efficacy;Asthma;Chinese medicine, traditional
摘要：
OBJECTIVE: To evaluate the self-efficacy and satisfaction of asthma patients subjected to summer acupoint application treatment (SAAT). METHODS: A two-year follow-up clinical study was conducted. Patients with asthma were treated by applying a herbal paste onto the Feishu (BL 13) and Fengmen (BL 12) acupoints on the three hottest days of summer, according to the traditional Chinese calendar, from 2008 to 2010. During a two-year follow-up, these patients were asked to fill out a questionnaire evaluating asthma degree, exacerbation frequency, concomitant medications and self-satisfaction. The self-rate and doctor-report outcomes obtained in parallel were evaluated to assess the efficacy of SAAT. RESULTS: A total of 527 asthma patients were initially enrolled in this study, of which 97 elderly patients and those with more severe cases of asthma were lost to follow-up. Thus, a total of 430 patients were valid for analysis using self-rate data. Nevertheless, occasional negative returns were obtained; almost all of the outcomes were rated as "No change", "Moderate effective", or "Very effective". In addition, 80% of the patients were satisfied with this treatment. Moreover, 391 (91.4%) patients were somewhat improved after SAAT in 2009, and further improvement was observed in 2010. After SAAT, the average asthma-degree score decreased from 5.3 in 2008 to 4 in 2009 and, subsequently to 3.5 in 2010. CONCLUSION: With pronounced patient satisfaction, SAAT can reduce the exacerbation severity and frequency, concomitant medications and asthma degree. Prolonging the treatment course might enhance the efficacy of SAAT. (C) 2015 JTCM. All rights reserved.
期刊：
International Journal of Lower Extremity wounds,2015年14(4):335-342 ISSN：1534-7346
通讯作者：
Fu, Xiao-Bing
作者机构：
[Dong, Liang; Liu, Jie-Jie; Han, Wei-Dong; Hao, Hao-Jie; Tong, Chuan; Li, Mei-Rong; Fu, Xiao-Bing; He, Wen-Jun; Chen, Li; Hou, Qian] Chinese Peoples Liberat Army Gen Hosp, Beijing 100048, Peoples R China.;[Hou, Qian] Chinese PLA 148th Hosp, Zibo, Peoples R China.;[Fu, Xiao-Bing; He, Wen-Jun] Chinese Peoples Liberat Army Gen Hosp, Affiliated Hosp 1, Beijing 100048, Peoples R China.;[Chen, Li] Hunan Univ Chinese Med, Changsha, Hunan, Peoples R China.;[Zhou, Zhong-Zhi] Hunan Univ Chinese Med, Affiliated Hosp 1, Changsha, Hunan, Peoples R China.
通讯机构：
[Fu, Xiao-Bing] C;Chinese Peoples Liberat Army Gen Hosp, Affiliated Hosp 1, Wound Healing & Cell Biol Lab, 51 Fucheng Rd, Beijing 100048, Peoples R China.
关键词：
wound healing;herbal medicine;collagen;diabetic mouse model
摘要：
Wound healing is a troublesome problem in diabetic patients. Besides, there is also an increased risk of postsurgical wound complications for diabetic patient. It has been revealed that traditional Chinese medicine may promote healing and inhibit scar formation, while the changes of morphology and physiology of wounds on such medicine treatment still remain elusive. In this study, we first used the ultralow temperature preparation method to produce mixed superfine powder from Agrimonia pilosa (A), Nelumbo nucifera (N), Boswellia carteri (B), and Pollen typhae (P), named as ANBP. Applying ANBP on 40 streptozotocin (STZ)-induced diabetic C57BL/6 mice (4-6 weeks, 20 ± 2 g), we observed that the wound healing process was accelerated and the wound healing time was shortened (14 days, P < .05). Pathological observation using hematoxylin–eosin staining indicated that inflammatory cells were reduced (P < .05) while the thickness of granulation tissue and length of epithelial tongue were increased (P < .05). The vascular density was increased on 7 and 14 days after ANBP treatment. Masson and Sirius red staining showed that, at the early stage of trauma, the expressions of Col I and Col III, especially Col III, were increased in the ANBP group (P < .05). Studies in vitro demonstrated that tubular formation was significantly increased after ANBP treatment on human vascular endothelial cells in a dose-dependent way. Taken together, our studies revealed that ANBP treatment could accelerate wound healing, promote vascularization, and inhibit inflammation, suggesting the potential clinic application of ANBP for diabetes mellitus and refractory wounds.
摘要：
A method of immobilizing genomic DNA on microcrystalline cellulose was described to isolate DNA-binding proteins. At first, DNA fragments were prepared by sonication and immobilized on cellulose phase. After the immobilization, DNA duplex formation was done. Using this microcrystalline cellulose affinity chromatography technique, DNA-binding proteins from kumquat (Fortunella margarita Swingle) leaf samples were isolated and then analyzed by Liquid Chromatography–Mass Spectrometry (LC-MS/MS). LC-MS/MS analysis showed that twenty-eight kinds of protein mainly including histones, protein-synthetic proteins and other DNA-binding proteins were identified. The identification list consists with the results in previous research on DNA-binding proteins isolation. It further suggests that the technique developed in this study can be applied to the effective isolation of DNA-binding proteins.
摘要：
Aims: The clinical significance of myeloperoxidase (MPO) has been the focus of investigation because it may contribute to the chronic, non-microbial inflammatory process in various diseases. Here, we determined serum MPO levels in rheumatoid arthritis (RA) and other autoimmune or inflammatory conditions, and investigated the associations between MPO levels and disease activity indicators in RA. Main methods: The distribution of MPO was determined in serum samples from patients with RA, systemic lupus erythematosus (SLE), primary Sjogren's syndrome (pSS), dermatomyositis (DM), or ankylosing spondylitis (AS) and from healthy controls using commercial ELISA kits. Associations of serum MPO levels with the disease variables of RA patients were evaluated. Key findings: All patient samples analyzed showed higher serum levels of MPO than healthy controls. Furthermore, MPO levels in RA were significantly higher than those in the other diseases with the exception of DM. Higher MPO levels were observed in RA patients with increased C-reactive protein (p = 0.005) or neutrophil percentage (p < 0.001), as well as in those with highly active disease (p < 0.001). Moderate positive correlations between MPO levels and IgM (r = 0.334, p = 0.001),C-reactive protein (r = 0.293, p = 0.003), erythrocyte sedimentation rate (r = 0.240, p = 0.016), or DAS28 (r = 0.350, p < 0.001) were also demonstrated. Significance: The MPO concentration is likely to increase in patients with chronic inflammation. The associations between MPO and the disease variables of RA patients support a role for MPO in the inflammatory process of the disease. (C) 2014 Elsevier Inc. All rights reserved.
