期刊：
MOLECULES AND CELLS,2025年48(6):100216 ISSN：1016-8478
通讯作者：
Yu, R
作者机构：
[Yu, Yun-Feng; Qin, Li-Na; Yu, Rong; Yu, R] Hunan Univ Chinese Med, Dept Endocrinol, Hosp 1, Changsha, Hunan, Peoples R China.;[Ma, Lie] Henan Univ Chinese Med, Dept Reprod Med, Affiliated Hosp 3, Zhengzhou, Henan, Peoples R China.;[Yu, Rong; Yu, R] Hunan Univ Chinese Med, Coll Chinese Med, Changsha, Hunan, Peoples R China.;[Yu, Rong; Yu, R] Hunan Univ Chinese Med, 300 Xueshi Rd, Changsha 410208, Hunan, Peoples R China.
通讯机构：
[Yu, R ] H;Hunan Univ Chinese Med, Dept Endocrinol, Hosp 1, Changsha, Hunan, Peoples R China.;Hunan Univ Chinese Med, Coll Chinese Med, Changsha, Hunan, Peoples R China.;Hunan Univ Chinese Med, 300 Xueshi Rd, Changsha 410208, Hunan, Peoples R China.
摘要：
Metabolic dysfunction-associated steatotic liver disease (MASLD) is a progressive disease that affects the health of approximately one-third of the world's population. It is the primary cause of end-stage liver disease, liver malignancy, and liver transplantation, resulting in a great medical burden. No medications have yet been approved by the US Food and Drug Administration (FDA) for treating MASLD without liver inflammation or scarring. Therefore, the development of specific drugs to treat MASLD remains a key task in the ongoing research objective. Extracellular vesicles (EVs) play an important role in the communication between organs, tissues, and cells. Recent studies have found that intestinal microbiota are closely related to the pathogenesis and progression of MASLD. EVs produced by bacteria (BEVs) play an indispensable role in this process. Thus, this study provides a new direction for MASLD treatment. However, the mechanism by which BEVs affect MASLD remains unclear. Therefore, this study investigated the influence and function of intestinal microbiota in MASLD. Additionally, we focus on the research progress of BEVs in recent years, and explain the relationship between BEVs and MASLD from the perspectives of glucose and lipid metabolism, immune responses, and intestinal homeostasis. Finally, we summarized the potential therapeutic value of BEVs and EVs from other sources, such as adipocytes, immunocytes, stem cells, and plants.
Metabolic dysfunction-associated steatotic liver disease (MASLD) is a progressive disease that affects the health of approximately one-third of the world's population. It is the primary cause of end-stage liver disease, liver malignancy, and liver transplantation, resulting in a great medical burden. No medications have yet been approved by the US Food and Drug Administration (FDA) for treating MASLD without liver inflammation or scarring. Therefore, the development of specific drugs to treat MASLD remains a key task in the ongoing research objective. Extracellular vesicles (EVs) play an important role in the communication between organs, tissues, and cells. Recent studies have found that intestinal microbiota are closely related to the pathogenesis and progression of MASLD. EVs produced by bacteria (BEVs) play an indispensable role in this process. Thus, this study provides a new direction for MASLD treatment. However, the mechanism by which BEVs affect MASLD remains unclear. Therefore, this study investigated the influence and function of intestinal microbiota in MASLD. Additionally, we focus on the research progress of BEVs in recent years, and explain the relationship between BEVs and MASLD from the perspectives of glucose and lipid metabolism, immune responses, and intestinal homeostasis. Finally, we summarized the potential therapeutic value of BEVs and EVs from other sources, such as adipocytes, immunocytes, stem cells, and plants.
期刊：
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY,2025年15:1539277 ISSN：2235-2988
通讯作者：
Peng, XX;Deng, Na;Deng, N
作者机构：
[Peng, Xinxin; Peng, XX; Fang, Leyao] Hunan Univ Chinese Med, Hosp 1, Changsha, Peoples R China.;[Shen, Junxi; Peng, Xinxin; Yi, Xin; Deng, Na; Peng, XX; Deng, N; Fang, Leyao] Hunan Univ Chinese Med, Domest Class Discipline Construction Project Chine, Changsha, Peoples R China.;[Shen, Junxi; Yi, Xin; Deng, N; Deng, Na; Fang, Leyao] Hunan Univ Chinese Med, Sch Tradit Chinese Med, Changsha, Peoples R China.
通讯机构：
[Deng, N ; Peng, XX ; Deng, N] H;Hunan Univ Chinese Med, Hosp 1, Changsha, Peoples R China.;Hunan Univ Chinese Med, Domest Class Discipline Construction Project Chine, Changsha, Peoples R China.;Hunan Univ Chinese Med, Sch Tradit Chinese Med, Changsha, Peoples R China.
摘要：
BACKGROUND: Constipation is a common digestive system disorder, which is closely related to the intestinal flora. Zhishi Daozhi decoction (ZDD) is a traditional Chinese medicine prescription used to treat constipation caused by indigestion. This study is to evaluate the efficacy of ZDD in treating constipation and to elucidate the underlying mechanism. METHODS: In this study, Kunming mice were administered a high-protein diet (HFHPD) and loperamide hydrochloride injections to induce constipation. The mice then received varying doses (2.4, 4.7, and 9.4 mg/kg) of ZDD for seven days. Following the sampling process, we measured fecal microbial activity. The levels of 5-hydroxytryptamine (5-HT), vasoactive intestinal peptide (VIP), and aquaporin-3 (AQP3) were quantified using enzyme-linked immunosorbent assay. Changes in the gut microbiota were evaluated through 16S rRNA gene sequencing. Additionally, we investigated the correlation between specific microbiota features and the levels of 5-HT, VIP, and AQP3. RESULTS: The fecal surface of the mice in the model group (CMM) was rough and dry. The stool of mice in the low-dose ZDD group (CLD), medium-dose ZDD group (CMD), and high-dose ZDD group (CHD) exhibited a smoother texture, closely resembling that of the normal group (CNM). 5-HT levels in the CMM group were significantly lower than in the CNM, CLD, and CHD. VIP levels in the CMD were lower than in the other four groups, and AQP3 levels in CMM showed a decreasing trend. The fecal microbial activity of the CMM group was significantly higher than that of the other groups. Diversity analysis indicated that CMD and CHD treatments were more effective in restoring the intestinal microbiota structure. Potential pathogenic bacteria, including Clostridium, Aerococcus, Jeotgalicoccus, and Staphylococcus were enriched in CMM. In contrast, beneficial bacteria such as Faecalibacterium, Bacillaceae, and Bacillus were more prevalent in the CLD, CMD, and CHD. Correlation analysis revealed that Streptococcus and Enterococcus were positively correlated with VIP, while Succinivibrio showed a negative correlation with 5-HT. CONCLUSIONS: Constipation induced by HFHPD and loperamide hydrochloride disrupts the structure of the intestinal microbiota. ZDD appears to alleviate constipation, potentially through mechanisms linked to the brain-gut axis and its interaction with the intestinal microbiota. Among the treatment groups, the medium dose of ZDD demonstrated the most effective results.
摘要：
BACKGROUND AND OBJECTIVES: To explore the mechanism of action of the differential components of medicinal and edible lilies in treating depression by network pharmacology using UPLC-Q-TOF-MS technology. METHODS: The chemical composition of medicinal and edible lilies was analyzed, screening for unique medicinal compounds. Searched for depression-related targets. Constructed PPI networks. Performed GO and KEGG analyses. Built a network of differential components, and conducted molecular docking. In addition, the contents of regaloside before and after lily processing were compared Results: Medicinal lilies and edible lilies have 17 main differences, including regaloside B and regaloside E. There are 179 targets for actives, 2690 for antidepressants, and 98 intersected. Core targets (7) led to 238 GO processes and 107 KEGG pathways. The molecular docking results showed that 17 components, including regaloside B, regaloside E, (25R)-3β,17α-Dihydroxy-5α- spirostan-6-one 3-O-α-L- rhamnopyranosyl-(1→2)-β- D-glucopyranoside (Named: Lilium lancifolium saponin), etc. could act on 7 potential targets such as EGFR, HSP90AA1, STAT3, TNF, etc. to exert antidepressant effects. CONCLUSION: This study employed a network pharmacology combined with a molecular docking approach to compare the active constituents of medicinal and edible lilies in antidepressants, and their pharmacological mechanisms, both theoretically and technically. The phytoconstituents were found to act mainly by inhibiting the inflammatory response in depression. Especially Lilium lancifolium saponin may have a close relationship with antidepressants. These results provide some justification for lilies in the treatment of depression.
作者机构：
[Huang, Xiangjun] Hunan Univ Chinese Med, Affiliated Hosp 1, Dept Gen Surg, Changsha, Peoples R China.;[Deng, Xinyue] Cent South Univ, Xiangya Sch Med, Changsha, Peoples R China.;[Xu, Hongbo; Yuan, Lamei; Yuan, LM; Deng, Hao; Deng, Xiong] Cent South Univ, Xiangya Hosp 3, Ctr Expt Med, Changsha, Peoples R China.
通讯机构：
[Yuan, LM ; Deng, H] C;Cent South Univ, Xiangya Hosp 3, Ctr Expt Med, Changsha, Peoples R China.
摘要：
The peroxisome biogenesis disorders (PBDs) are a group of rare inherited autosomal recessive diseases characterized by motor and cognitive neurological dysfunction, hypotonia, seizures, feeding difficulties, retinopathy, sensorineural hearing loss, hepatic and renal abnormalities, and chondrodysplasia punctata of long bones, and the clinical expression is variable. Exome sequencing and Sanger sequencing were used to identify the genetic defect for PBDs in a two-generation non-consanguineous Han-Chinese pedigree. Compound heterozygous variants, a novel splicing variant c.113-2A>G and a reported substitution c.890T>C (p.Leu297Pro), in the peroxisomal biogenesis factor 10 gene (PEX10) were detected. The splicing variant c.113-2A>G led to a canonical splice acceptor site inactivation, exon 2 skipping, and in-frame deletions (p.Ala39_Gly65del). The three patients had similar phenotypes of milder PBDs, which were further genetically determined as PBD6B. The findings extend the PEX10 variant spectrum and may provide new insights into PBDs causation and diagnosis, with implications for genetic counseling and clinical management.
期刊：
Frontiers in Pharmacology,2025年16:1417603 ISSN：1663-9812
通讯作者：
Fu, W;Zhou, Q
作者机构：
[You, Xujun] Hunan Univ Chinese Med, Grad Sch, Changsha, Peoples R China.;[Li, Qixin; Fu, Wei; You, Xujun] Guangzhou Univ Chinese Med, Shenzhen Baoan Tradit Chinese Med Hosp, Dept Androl, Shenzhen, Peoples R China.;[Wu, Yongrong] Hunan Univ Chinese Med, Coll Integrated Tradit Chinese & Western Med, Changsha, Peoples R China.;[Sheng, Wen] Hunan Univ Chinese Med, Androl Lab, Changsha, Peoples R China.;[Zhou, Qing; Zhou, Q] Hunan Univ Chinese Med, Dept Androl, Affiliated Hosp 1, Changsha, Peoples R China.
通讯机构：
[Fu, W ] G;[Zhou, Q ] H;Guangzhou Univ Chinese Med, Shenzhen Baoan Tradit Chinese Med Hosp, Dept Androl, Shenzhen, Peoples R China.;Hunan Univ Chinese Med, Dept Androl, Affiliated Hosp 1, Changsha, Peoples R China.
关键词：
prostate cancer;autophagy;PI3K/AKT;Astragalus–Scorpio;Astragaloside IV;polypeptide extract from scorpion venom
摘要：
Tumor cells inhibit immune activation pathways and antigen presentation processes through epigenetic silencing mechanisms, thereby lowering the sensitivity of hepatocellular carcinoma (HCC) to radio-immunotherapy. Herein, a tumor-specific, multifunctional nanotherapeutic platform integrating epigenetic regulation with radiotherapy is developed. Radiotherapy activates both cGAS-STING and pyroptosis pathways in HCC cells, while concurrently restoring antigen presentation functions. Specifically, a PEGylated hollow mesoporous MnO 2 (HM-PEG) nanoparticle is loaded with the DNA methylation inhibitor decitabine (DAC). In acidic tumor microenvironment (TME), the HM-PEG/D nanoshell dissociates, releasing DAC and Mn ions, which effectively counteract the hypoxic TME. DAC upregulates the expression of cGAS, gasdermin E (GSDME), and MHC I in HCC cells, while Mn ions facilitate image-guided radiotherapy and sensitize the cGAS-STING pathway. The alleviation of hypoxia further enhances radiotherapy sensitivity, promotes DNA damage, activates the cGAS-STING pathway, and triggers caspase-3 activation, leading to GSDME-dependent pyroptosis. Subsequently, the released interferons and inflammatory signals foster the maturation of dendritic cells, thereby activating CD8 + T cell-mediated anti-tumor immune responses. The upregulation of MHC I enhances tumor cell susceptibility to recognition and cytotoxic killing by CD8 + T cells. Therefore, this approach overcomes critical barriers within the TME, bridging innate and adaptive immunity, and enables potent image-guided HCC-targeted radio-immunotherapy.
Tumor cells inhibit immune activation pathways and antigen presentation processes through epigenetic silencing mechanisms, thereby lowering the sensitivity of hepatocellular carcinoma (HCC) to radio-immunotherapy. Herein, a tumor-specific, multifunctional nanotherapeutic platform integrating epigenetic regulation with radiotherapy is developed. Radiotherapy activates both cGAS-STING and pyroptosis pathways in HCC cells, while concurrently restoring antigen presentation functions. Specifically, a PEGylated hollow mesoporous MnO 2 (HM-PEG) nanoparticle is loaded with the DNA methylation inhibitor decitabine (DAC). In acidic tumor microenvironment (TME), the HM-PEG/D nanoshell dissociates, releasing DAC and Mn ions, which effectively counteract the hypoxic TME. DAC upregulates the expression of cGAS, gasdermin E (GSDME), and MHC I in HCC cells, while Mn ions facilitate image-guided radiotherapy and sensitize the cGAS-STING pathway. The alleviation of hypoxia further enhances radiotherapy sensitivity, promotes DNA damage, activates the cGAS-STING pathway, and triggers caspase-3 activation, leading to GSDME-dependent pyroptosis. Subsequently, the released interferons and inflammatory signals foster the maturation of dendritic cells, thereby activating CD8 + T cell-mediated anti-tumor immune responses. The upregulation of MHC I enhances tumor cell susceptibility to recognition and cytotoxic killing by CD8 + T cells. Therefore, this approach overcomes critical barriers within the TME, bridging innate and adaptive immunity, and enables potent image-guided HCC-targeted radio-immunotherapy.
摘要：
Ischemic stroke ranks as the second leading cause of global mortality and disability. Although reperfusion is crucial for salvaging brain tissue, it carries the risk of secondary injuries, such as ferroptosis. Gastrodin, a neuroprotective compound found in Chinese herbal medicine, may regulate this process. However, its impact on stroke-induced ferroptosis remains unclear. Objective: This research endeavors to probe Gastrodin's influence on post-ischemic ferroptosis, deciphering its mechanisms and assessing its therapeutic promise. Methods: We developed rat models of middle cerebral artery occlusion/reperfusion (MCAO/R) and created oxygen-glucose deprivation/reoxygenation (OGD/R)-damaged PC12 cell models. Gastrodin was administered to assess ferroptosis using Prussian blue staining and fluorescence probes. To investigate the effects of gastrodin on the xCT/GPX4 and ACSL4/LPCAT3 pathways, we employed molecular docking, immunofluorescence, Western blotting, and quantitative real-time polymerase chain reaction (qRT-PCR). Additionally, we used transmission electron microscopy and JC-1 fluorescence probes to examine mitochondrial integrity and function. Results: Our study demonstrated that gastrodin significantly reduced iron accumulation and lipid peroxidation in the brains of MCAO/R rats and OGD/R-injured PC12 cells. It suppressed reactive oxygen species (ROS) and ameliorated mitochondrial membrane potential. It potentiates the xCT/GPX4 axis while repressing the ACSL4/LPCAT3 pathway, leading to improved mitochondrial architecture and function, notably characterized by decreased mitochondrial membrane potential, reduced ROS levels, and increased formation of mitochondrial cristae. By modulating the xCT/GPX4 and ACSL4/LPCAT3 pathways, gastrodin mitigated ferroptosis in ischemic stroke, thereby preserving mitochondrial structural and functional integrity. This study provides novel mechanistic insights into gastrodin's therapeutic potential for treating ischemic stroke, highlighting the importance of traditional Chinese medicine in modern medical therapy.
Ischemic stroke ranks as the second leading cause of global mortality and disability. Although reperfusion is crucial for salvaging brain tissue, it carries the risk of secondary injuries, such as ferroptosis. Gastrodin, a neuroprotective compound found in Chinese herbal medicine, may regulate this process. However, its impact on stroke-induced ferroptosis remains unclear.
Objective: This research endeavors to probe Gastrodin's influence on post-ischemic ferroptosis, deciphering its mechanisms and assessing its therapeutic promise.
Methods: We developed rat models of middle cerebral artery occlusion/reperfusion (MCAO/R) and created oxygen-glucose deprivation/reoxygenation (OGD/R)-damaged PC12 cell models. Gastrodin was administered to assess ferroptosis using Prussian blue staining and fluorescence probes. To investigate the effects of gastrodin on the xCT/GPX4 and ACSL4/LPCAT3 pathways, we employed molecular docking, immunofluorescence, Western blotting, and quantitative real-time polymerase chain reaction (qRT-PCR). Additionally, we used transmission electron microscopy and JC-1 fluorescence probes to examine mitochondrial integrity and function.
Results: Our study demonstrated that gastrodin significantly reduced iron accumulation and lipid peroxidation in the brains of MCAO/R rats and OGD/R-injured PC12 cells. It suppressed reactive oxygen species (ROS) and ameliorated mitochondrial membrane potential. It potentiates the xCT/GPX4 axis while repressing the ACSL4/LPCAT3 pathway, leading to improved mitochondrial architecture and function, notably characterized by decreased mitochondrial membrane potential, reduced ROS levels, and increased formation of mitochondrial cristae.
By modulating the xCT/GPX4 and ACSL4/LPCAT3 pathways, gastrodin mitigated ferroptosis in ischemic stroke, thereby preserving mitochondrial structural and functional integrity. This study provides novel mechanistic insights into gastrodin's therapeutic potential for treating ischemic stroke, highlighting the importance of traditional Chinese medicine in modern medical therapy.
摘要：
Atherosclerosis (AS) is increasingly recognized as a chronic inflammatory disease that significantly compromises vascular health and serves as a major contributor to cardiovascular diseases. KCTD10, a protein implicated in a variety of biological processes, has garnered significant attention for its role in cardiovascular diseases and metabolic regulation. As a member of the KCTD protein family, KCTD10 is characterized by the presence of a T1 domain that interacts with voltage-gated potassium channels, a critical interaction for modulating channel activity and intracellular signal transduction. In our study, KCTD10 was identified as a focal point through an integrative analysis of differentially expressed genes (DEGs) across multiple datasets (GSE43292 and GSE9820) from the GEO database, aligned with immune-related gene sets from the ImmPort database. Advanced analytical tools, including Lasso regression and Support Vector Machine-Recursive Feature Elimination (SVM-RFE), were employed to refine our gene selection. We further applied Gene Set Enrichment Analysis (GSEA) and Gene Set Variation Analysis (GSVA) to these gene sets, revealing significant enrichment in immune-related pathways. The relationship between KCTD10 expression and immune processes was examined using CIBERSORT and ESTIMATE algorithms to assess tumor microenvironment characteristics, suggesting increased immune cell infiltration associated with higher KCTD10 expression. Validation of these findings was conducted using data from the GSE9820 dataset. Among 10 DEGs linked with KCTD10, 13 were identified as hub genes through LASSO and SVM-RFE analyses. Functional assays highlighted KCTD10's role in enhancing viral defense mechanisms, cytokine production, and immune cascades. Notably, KCTD10 expression correlated positively with several immune cells, including naive CD4 + T cells, eosinophils, resting NK cells, neutrophils, M0 macrophages, and particularly M1 macrophages, indicating a significant association. This research elucidates the complex relationship between KCTD10 and AS, underscoring its potential as a novel biomarker for diagnosing and monitoring the disease. Our findings provide a solid foundation for further investigations, suggesting that targeting KCTD10-related pathways could markedly advance our understanding and management of AS, offering new avenues for therapeutic intervention.
期刊：
Radiotherapy and Oncology,2025年205:110770 ISSN：0167-8140
通讯作者：
Xing, Y
作者机构：
[Xing, Yan; Gu, Qianbiao] Xinjiang Med Univ, Affiliated Hosp 1, Imaging Ctr, Urumqi 830011, Peoples R China.;[Sun, Huiling] Tradit Chinese Med Hosp Changji Hui Autonomous Pre, Dept CT & MR, Changji 831100, Changji Hui Aut, Peoples R China.;[Liu, Peng] Hunan Normal Univ, Hunan Prov Peoples Hosp, Affiliated Hosp 1, Dept Radiol, Changsha 410000, Peoples R China.;[Hu, Xiaoli] Hunan Univ Chinese Med, Affiliated Hosp 1, Dept Radiol, Changsha 410000, Peoples R China.;[Yang, Jiankang] YueYang Cent Hosp, Dept Radiol, Yueyang 414000, Peoples R China.
通讯机构：
[Xing, Y ] X;Xinjiang Med Univ, Affiliated Hosp 1, Imaging Ctr, Urumqi 830011, Peoples R China.
关键词：
Deep learning radiomics;Imaging biomarker;Pancreatic ductal adenocarcinoma (PDAC);Prognosis
摘要：
Purpose This multicenter study aimed to develop and validate a multiscale deep learning radiomics nomogram for predicting recurrence-free survival (RFS) in patients with pancreatic ductal adenocarcinoma (PDAC).
This multicenter study aimed to develop and validate a multiscale deep learning radiomics nomogram for predicting recurrence-free survival (RFS) in patients with pancreatic ductal adenocarcinoma (PDAC).
Materials and methods A total of 469 PDAC patients from four hospitals were divided into training and test sets. Handcrafted radiomics and deep learning (DL) features were extracted from optimal regions of interest, encompassing both intratumoral and peritumoral areas. Univariate Cox regression, LASSO regression, and multivariate Cox regression selected features for three image signatures (intratumoral, peritumoral radiomics, and DL). A multiscale nomogram was constructed and validated against the 8th AJCC staging system.
A total of 469 PDAC patients from four hospitals were divided into training and test sets. Handcrafted radiomics and deep learning (DL) features were extracted from optimal regions of interest, encompassing both intratumoral and peritumoral areas. Univariate Cox regression, LASSO regression, and multivariate Cox regression selected features for three image signatures (intratumoral, peritumoral radiomics, and DL). A multiscale nomogram was constructed and validated against the 8th AJCC staging system.
Results The 4 mm peritumoral VOI yielded the best radiomics prediction, while a 15 mm expansion was optimal for deep learning. The multiscale nomogram demonstrated a C-index of 0.82 (95 % CI: 0.78–0.85) in the training set and 0.70 (95 % CI: 0.64–0.76) in the external test 1 (high-volume hospital), with the external test 2 (low-volume hospital) showing a C-index of 0.78 (95 % CI: 0.65–0.91). These outperformed the AJCC system’s C-index (0.54–0.57). The area under the curve (AUC) for recurrence prediction at 0.5, 1, and 2 years was 0.89, 0.94, and 0.89 in the training set, with AUC values ranging from 0.75 to 0.97 in the external validation sets, consistently surpassing the AJCC system across all sets. Kaplan-Meier analysis confirmed significant differences in prognosis between high- and low-risk groups (P < 0.01 across all cohorts).
The 4 mm peritumoral VOI yielded the best radiomics prediction, while a 15 mm expansion was optimal for deep learning. The multiscale nomogram demonstrated a C-index of 0.82 (95 % CI: 0.78–0.85) in the training set and 0.70 (95 % CI: 0.64–0.76) in the external test 1 (high-volume hospital), with the external test 2 (low-volume hospital) showing a C-index of 0.78 (95 % CI: 0.65–0.91). These outperformed the AJCC system’s C-index (0.54–0.57). The area under the curve (AUC) for recurrence prediction at 0.5, 1, and 2 years was 0.89, 0.94, and 0.89 in the training set, with AUC values ranging from 0.75 to 0.97 in the external validation sets, consistently surpassing the AJCC system across all sets. Kaplan-Meier analysis confirmed significant differences in prognosis between high- and low-risk groups (P < 0.01 across all cohorts).
Conclusion The multiscale nomogram effectively stratifies recurrence risk in PDAC patients, enhancing presurgical clinical decision-making and potentially improving patient outcomes.
The multiscale nomogram effectively stratifies recurrence risk in PDAC patients, enhancing presurgical clinical decision-making and potentially improving patient outcomes.
摘要：
Ulcerative colitis (UC) is a chronic idiopathic inflammatory disease affecting the colon and rectum. Characterized by recurrent attacks, UC is often resistant to traditional anti-inflammatory therapies, imposing significant physiological, psychological, and economic burdens on patients. In light of these challenges, innovative targeted therapies have become a new expectation for patients with UC. A crucial pathological feature of UC is the impairment of the intestinal mucosal barrier, which underlies aberrant immune responses and inflammation. Intestinal stem cells (ISCs), which differentiate into intestinal epithelial cells, play a central role in maintaining this barrier. Growing studies have proved that regulating the regeneration and differentiation of ISC is a promising approach to treating UC. Despite this progress, there is a dearth of comprehensive articles describing the role of ISCs in UC. This review focuses on the importance of ISCs in maintaining the intestinal mucosal barrier in UC and discusses the latest findings on ISC functions, markers, and their regulatory mechanisms. Key pathways involved in ISC regulation, including the Wnt, Notch, Hedgehog (HH), Hippo/Yap, and autophagy pathways, are explored in detail. Additionally, this review examines recent advances in ISC-targeted therapies for UC, such as natural or synthetic compounds, microbial preparations, traditional Chinese medicine (TCM) extracts and compounds, and transplantation therapy. This review aims to offer novel therapeutic insights and strategies for patients who have long struggled with UC.
作者机构：
[Yi, Xi; Che, Yilei] Hunan Normal Univ, Hunan Prov Peoples Hosp, Affiliated Hosp 1, Dept Radiol, Changsha 410016, Peoples R China.;[Yang, Yu] Hunan Univ Chinese Med, Hosp 1, Dept Radiol, Changsha 410007, Peoples R China.
通讯机构：
[Yi, X ] H;Hunan Normal Univ, Hunan Prov Peoples Hosp, Affiliated Hosp 1, Dept Radiol, Changsha 410016, Peoples R China.
关键词：
Breast cancer;Kaiser score;Predictive model;Nomogram;Magnetic resonance imaging (MRI)
摘要：
Rationale and Objectives This study aims to develop and validate a new diagnostic model based on the Kaiser score for preoperative diagnosis of the malignancy probability of enhancing lesions on breast MRI.
This study aims to develop and validate a new diagnostic model based on the Kaiser score for preoperative diagnosis of the malignancy probability of enhancing lesions on breast MRI.
Materials and Methods This study collected consecutive inpatient data (including imaging data, clinical data, and pathological data) from two different institutions. All patients underwent preoperative breast Dynamic Contrast-Enhanced Magnetic Resonance Imaging (DCE-MRI) examinations and were found to have enhancing lesions. These lesions were confirmed as benign or malignant by surgical resection or biopsy pathology (all carcinomas in situ were confirmed by pathology after surgical resection). Data from one institution were used as the training set(284 cases), and data from the other institution were used as the validation set(107 cases). The Kaiser score was directly incorporated into the diagnostic model as a single predictive variable. Other predictive variables were screened using Least Absolute Shrinkage and Selection Operator (LASSO) regression. Multivariate logistic regression was employed to integrate the Kaiser score and other selected predictive variables to construct a new diagnostic model, presented in the form of a nomogram. Receiver operating characteristic (ROC) curve, DeLong test, net reclassification improvement (NRI), and integrated discrimination improvement (IDI) were adopted to evaluate and compare the discrimination of the diagnostic model for breast enhancing lesions based on Kaiser score (hereinafter referred to as the “breast lesion diagnostic model”) and the Kaiser score alone. Calibration curves were used to assess the calibration of the breast lesion diagnostic model, and decision curve analysis (DCA) was used to evaluate the clinical efficacy of the diagnostic model and the Kaiser score.
This study collected consecutive inpatient data (including imaging data, clinical data, and pathological data) from two different institutions. All patients underwent preoperative breast Dynamic Contrast-Enhanced Magnetic Resonance Imaging (DCE-MRI) examinations and were found to have enhancing lesions. These lesions were confirmed as benign or malignant by surgical resection or biopsy pathology (all carcinomas in situ were confirmed by pathology after surgical resection). Data from one institution were used as the training set(284 cases), and data from the other institution were used as the validation set(107 cases). The Kaiser score was directly incorporated into the diagnostic model as a single predictive variable. Other predictive variables were screened using Least Absolute Shrinkage and Selection Operator (LASSO) regression. Multivariate logistic regression was employed to integrate the Kaiser score and other selected predictive variables to construct a new diagnostic model, presented in the form of a nomogram. Receiver operating characteristic (ROC) curve, DeLong test, net reclassification improvement (NRI), and integrated discrimination improvement (IDI) were adopted to evaluate and compare the discrimination of the diagnostic model for breast enhancing lesions based on Kaiser score (hereinafter referred to as the “breast lesion diagnostic model”) and the Kaiser score alone. Calibration curves were used to assess the calibration of the breast lesion diagnostic model, and decision curve analysis (DCA) was used to evaluate the clinical efficacy of the diagnostic model and the Kaiser score.
Results LASSO regression indicated that, besides the indicators already included in the Kaiser score system, "age", "MIP sign", "associated imaging features", and "clinical breast examination (CBE) results" were other valuable diagnostic parameters for breast enhancing lesions. In the training set, the AUCs of the breast lesion diagnostic model and the Kaiser score were 0.948 and 0.869, respectively, with a statistically significant difference (p < 0.05). In the validation set, the AUCs of the breast lesion diagnostic model and the Kaiser score were 0.956 and 0.879, respectively, with a statistically significant difference (p < 0.05). The DeLong test, NRI, and IDI showed that the breast lesion diagnostic model had a higher discrimination ability for breast enhancing lesions compared to the Kaiser score alone, with statistically significant differences (p < 0.05). The calibration curves indicated good calibration of the breast lesion diagnostic model. DCA demonstrated that the breast lesion diagnostic model had higher clinical application value, with greater net clinical benefit over a wide range of diagnostic thresholds compared to the Kaiser score.
LASSO regression indicated that, besides the indicators already included in the Kaiser score system, "age", "MIP sign", "associated imaging features", and "clinical breast examination (CBE) results" were other valuable diagnostic parameters for breast enhancing lesions. In the training set, the AUCs of the breast lesion diagnostic model and the Kaiser score were 0.948 and 0.869, respectively, with a statistically significant difference (p < 0.05). In the validation set, the AUCs of the breast lesion diagnostic model and the Kaiser score were 0.956 and 0.879, respectively, with a statistically significant difference (p < 0.05). The DeLong test, NRI, and IDI showed that the breast lesion diagnostic model had a higher discrimination ability for breast enhancing lesions compared to the Kaiser score alone, with statistically significant differences (p < 0.05). The calibration curves indicated good calibration of the breast lesion diagnostic model. DCA demonstrated that the breast lesion diagnostic model had higher clinical application value, with greater net clinical benefit over a wide range of diagnostic thresholds compared to the Kaiser score.
Conclusion The Kaiser score-based breast lesion diagnostic model, which integrates "age," "MIP sign", "associated imaging features", and "CBE results", can be used for the preoperative diagnosis of the malignancy probability of breast enhancing lesions, and it outperforms the classic Kaiser score in terms of diagnostic performance for such lesions.
The Kaiser score-based breast lesion diagnostic model, which integrates "age," "MIP sign", "associated imaging features", and "CBE results", can be used for the preoperative diagnosis of the malignancy probability of breast enhancing lesions, and it outperforms the classic Kaiser score in terms of diagnostic performance for such lesions.
期刊：
BMJ Open Quality,2025年14(1):e003037 ISSN：2399-6641
通讯作者：
Liu, D
作者机构：
[Liu, Dan; Yang, Lin; Wen, Ting; Pi, Guifang] Hunan Univ Chinese Med, Rheumatol & Immunol Dept, Hosp 1, Changsha, Hunan, Peoples R China.;[Xiang, Peng] Hunan Univ Chinese Med, Dept Otorhinolaryngol, Hosp 1, Changsha, Hunan, Peoples R China.;[Liu, Li] Hunan Univ Chinese Med, Telemed Ctr, Hosp 1, Changsha, Hunan, Peoples R China.
通讯机构：
[Liu, D ] H;Hunan Univ Chinese Med, Rheumatol & Immunol Dept, Hosp 1, Changsha, Hunan, Peoples R China.
关键词：
Chronic disease management;Continuity of Patient Care;Long-Term Care;META-ANALYSIS
摘要：
OBJECTIVES: This study sought to assess the effectiveness of nurse-led care (NLC) in patients with rheumatoid arthritis (RA). METHODS: We conducted a comprehensive search of the Cochrane Library, Web of Science, PubMed, Embase, CINAHL, ClinicalTrials.gov databases and the references from relevant literature published prior to May 2023. Two independent reviewers assessed the studies using population/intervention/comparison/outcome/study criteria, and quantitative tools were used to gauge the methodological quality of the included studies. Independent quality assessments were carried out using the Cochrane Collaboration's risk-of-bias tool. Effect sizes were determined using mean difference (MD) or standardised mean difference (SMD) with corresponding 95% CIs. RESULTS: Ultimately, 14 articles were included, encompassing a total of 3369 RA patients. NLC exhibited significant advantages in the primary outcome, disease activity (MD =-0.09, 95% CI (-0.17 to -0.01)), and the secondary outcome, self-efficacy (MD=0.40, 95% CI (0.03, 0.77)). In subgroup analysis, NLC was more effective in reducing disease activity compared with usual care (MD=-0.15, 95% CI (-0.26 to -0.04)), and there was no significant difference in disease activity reduction between NLC and rheumatologist-led care (MD=-0.02, 95% CI (-0.14, 0.10)). In terms of self-efficacy, no significant difference was observed between NLC and usual care (SMD=0.56, 95% CI (-0.09, 1.21)) or between NLC and rheumatologist-led care (SMD=0.20, 95% CI (-0.19, 0.59). When comparing other secondary outcomes (pain, satisfaction, quality of life, fatigue, stiffness, physical function and psychological status), the effectiveness of NLC for RA patients was similar to that of the control group, with no statistically significant differences. CONCLUSIONS: NLC proves highly effective in managing RA patients, surpassing usual care and equating to rheumatologist-led care in primary and some secondary outcomes. It may be feasible to allow nurses to participate in the disease management of some RA patients instead of doctors. PROSPERO REGISTRATION NUMBER: CRD42022362071.
期刊：
AGING CLINICAL AND EXPERIMENTAL RESEARCH,2025年37(1):1-16 ISSN：1594-0667
通讯作者：
Wen, Zhi;Chen, Q
作者机构：
[Guo, Cheng; Wen, Zhi; Chen, Qing; He, Fangyi; Kuang, Gaoyan; Lan, Xiangzhou; Li, Lingjia; Jia, Qing; Chen, Miao] Hunan Univ Tradit Chinese Med, Affiliated Hosp 1, Changsha 410007, Hunan, Peoples R China.;[He, Fangyi; Li, Lingjia; Jia, Qing] Hunan Univ Chinese Med, Sch Nursing, Changsha 410208, Hunan, Peoples R China.;[Zeng, Weike] Changsha Modern Nurse Magazine Co LTD, Changsha 410011, Hunan, Peoples R China.
通讯机构：
[Wen, Z; Chen, Q ] H;Hunan Univ Tradit Chinese Med, Affiliated Hosp 1, Changsha 410007, Hunan, Peoples R China.
关键词：
Knee osteoarthritis;Physical modalities therapy;Network meta-analysis;Pain relief;Joint function
摘要：
OBJECTIVE: This network meta-analysis (NMA) aimed to compare the efficacy of various physical modalities in alleviating pain, stiffness, and functional impairment in patients with knee osteoarthritis (KOA). METHODS: In accordance with PRISMA-P guidelines, we systematically searched nine databases(CNKI, VIP Database, Wanfang Database, SinoMed, PubMed, Embase, CINAHL, Web of Science, and the Cochrane Library) from inception to October 2024 to identify randomized controlled trials (RCTs) evaluating physical therapies for KOA. The interventions assessed included electrical stimulation therapy (EST), low-level light therapy (LLLT), thermotherapy (TT), cryotherapy (CT), and extracorporeal shock wave therapy (ESWT), with resistance and range of motion exercises (RRE) serving as comparators. Outcome measures comprised the Visual Analog Scale (VAS), Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC), and 6-minute walk test (6 MWT). Bayesian network meta-analyses and pairwise meta-analyses were performed using Stata 17.0 and R 4.4.1 software. RESULTS: 32 RCTs involving 2,078 participants were included. LLLT demonstrated the highest efficacy for pain reduction (VAS: MD=-3.32, 95% CI:-3.82 to-0.75; WOMAC pain: MD=-3.74, 95% CI:-6.68 to-0.72) and joint function improvement (SUCRA = 79.8). ESWT ranked second for pain relief (VAS: MD=-1.31, 95% CI:-2.42 to-0.16) and mobility enhancement (6 MWT: SUCRA = 71.5), while TT showed superior efficacy in reducing stiffness (WOMAC stiffness: MD=-2.09, 95%CI:-3.06 to-0.94; SUCRA = 98.1). In contrast, ultrasonic therapy (UT) did not provide significant benefits. CONCLUSIONS: The findings suggest that LLLT and ESWT may be optimal for pain relief and functional improvement in patients with KOA, whereas TT appears to be the most effective in reducing stiffness. Optimal dosing parameters of these physical modalities are crucial for maximizing clinical benefits. Clinicians should individualize treatment strategies based on patient-specific factors. Future large-scale RCTs are warranted to validate these protocols and address the heterogeneity of existing evidence. CLINICAL TRIAL NUMBER: Not applicable.
期刊：
Frontiers in Oral Health,2025年6:1568252 ISSN：2673-4842
通讯作者：
Liu, HY;Liao, RY;Hu, C
作者机构：
[Tang, Feng; He, Yinghui; Liu, Hongyu] Hunan Univ Chinese Med, Hosp 1, Dept Pharm, Changsha, Peoples R China.;[Liao, Ruoyi; Liao, RY; He, Yinghui] Hunan Univ Chinese Med, Hosp 1, Dept Nursing, Changsha, Peoples R China.;[Hu, Chun; Hu, C] Hunan Univ Chinese Med, Hosp 1, Dept Stomatol, Changsha, Peoples R China.
通讯机构：
[Liao, RY ; Liu, HY ; Hu, C ] H;Hunan Univ Chinese Med, Hosp 1, Dept Pharm, Changsha, Peoples R China.;Hunan Univ Chinese Med, Hosp 1, Dept Nursing, Changsha, Peoples R China.;Hunan Univ Chinese Med, Hosp 1, Dept Stomatol, Changsha, Peoples R China.
摘要：
OBJECTIVES: This randomized controlled trial aimed to investigate the impact of different telephone follow-up frequencies on periodontal clinical parameters after non-surgical periodontal therapy. MATERIALS AND METHODS: Patients with Stage II-IV periodontitis were enrolled and randomly assigned to high-frequency (once every 2 weeks), medium-frequency (once a month), and low-frequency (once in 3 months) follow-up groups. All patients received standard non-surgical periodontal treatment. The full mouth probing depth (PD), clinical attachment loss (CAL), gingival index (GI), and plaque index (PI) were evaluated at baseline, after treatment (T1) and post treatment 3 months (T2). RESULTS: From T1 to T2, the high-frequency follow-up group had significant reduced in PD (p = 0.03), improved in GI (p = 0.04) and PI (p = 0.03) compared with the medium and low-frequency groups. There was no significant difference in PD, GI, and PI between the medium-frequency group and the low-frequency group. No statistical difference was found in CAL among the three groups. CONCLUSION: More frequent telephone follow-up helps maintain and enhance non-surgical periodontal therapy effects.
摘要：
Background: The increasing prevalence of myopia among Chinese children and adolescents, especially at younger ages, has emerged as a significant concern in recent years. Pre-myopia is a key period for myopia prevention and control in children and adolescents. Previous studies suggested auricular acupressure (AA) therapy might offer a viable approach to prevent and slow down myopia progression. Nonetheless, these studies lack robust, high-quality, large-scale, multi-center evidence to conclusively support such assertions. The purpose of this research is to evaluate the efficacy, safety, and economic benefits of AA therapy in preventing myopia in Chinese children aged 6–12 years with pre-myopia. Methods: The single-blind, multi-center, parallel-group, randomized controlled trial will involve 318 pre-myopic children from 20 different centers across China. After recruitment, these participants will be randomly assigned to two groups (the AA group and the control group) at a 1:1 ratio. The AA group will receive auricular point sticking therapy along with health education for a period of 24 weeks, while the control group will be provided the sham AA treatment and routine health education. The assessments of outcomes will be conducted at the start of this study, and then after 4, 8, 12, and 24 weeks. The primary outcome is the change in spherical equivalent refraction at various follow-up times. The secondary outcomes include the number of myopia cases, uncorrected visual acuity, axial length, corneal curvature radius, accommodation amplitude, retinal and choroidal thickness, and eye behavior management. In addition, the cost-effectiveness analysis will be used as the evaluation index for economic assessment. Discussion: The results of this research will provide evidence on the efficacy, safety, and economic benefits of AA therapy in preventing myopia among children aged 6–12 years with pre-myopia in China.
摘要：
The present study aimed to explore the role of mitochondria‑associated membranes (MAMs) as a key interface between mitochondria and the endoplasmic reticulum (ER) and to evaluate their importance in maintaining the physiological functions of these two organelles. MAMs not only act as a structural bridge between mitochondria and the ER but also widely participate in the regulation of mitochondrial biosynthesis and function, Ca(2+) signal transduction, lipid metabolism, oxidative stress response and autophagy. In addition, the specific protein composition of MAMs is increasingly being recognized as having a profound impact on their function, and these proteins play a central role in regulating intercellular communication. Recently, the scientific community has accumulated a large amount of evidence supporting MAMs as potential targets for cardiovascular disease treatment. The present review focuses on the fine structure and multifunctional properties of MAMs and their mechanisms in the occurrence and development of cardiovascular diseases. The goal is to explore the mechanism of MAMs, therapeutic intervention points directly related to cardiovascular diseases, and feasibility of incorporating MAMs into the diagnostic strategy and treatment plan of cardiovascular diseases to provide novel insights and theoretical support for clinical practice in this field. MAMs have great potential as therapeutic targets for various cardiovascular diseases. This finding not only deepens the understanding of the interaction between organelles but also opens up a promising research path for the development of new therapeutic strategies for cardiovascular diseases.
摘要：
This study aimed to evaluate the causal effects of different immune cells on heart failure (HF) using Mendelian randomization (MR). Datasets for immune cell phenotypes and HF were obtained from European Bioinformatics Institute and FinnGen. Then, single nucleotide polymorphisms were screened according to the basic assumptions of MR. Subsequently, inverse variance weighted was used as primary tool for MR analysis, and Cochran Q and leave-one-out analyses were used to assess heterogeneity and robustness, respectively. MR analysis showed that cluster of differentiation (CD) 66b++ myeloid cell absolute count (AC) (odds ratio [OR] 1.043, 95% confidence interval [CI] 1.001-1.088, P = .045), human leukocyte antigen D-related on CD14- CD16+ monocyte (OR 1.030, 95% CI 1.005-1.056, P = .019), IgD on unsw mem (OR 1.046, 95% CI 1.015-1.078, P = .003), CD4 on CD4+ (OR 1.039, 95% CI 1.009-1.070, P = .011), CD24 on IgD+ CD38- (OR 1.026, 95% CI 1.000-1.052, P = .046), CD20 on CD24 + CD27+ (OR 1.032, 95% CI 1.003-1.061, P = .029), CD19 on CD20- (OR 1.037, 95% CI 1.005-1.071, P = .023), CD62L- CD86 + myeloid dendritic cell %DC (OR 1.032, 95% CI 1.004-1.061, P = .027), human leukocyte antigen D-related + CD4 + AC (OR 1.037, 95% CI 1.003-1.072, P = .032), and effector memory CD8br AC (OR 1.048, 95% CI 1.021-1.076, P < .001) were associated with increased genetic susceptibility to HF. Cochran Q and sensitivity analyses showed that the results had no heterogeneity and were robust. This MR analysis revealed 10 immune cell phenotypes associated with increased genetic susceptibility to HF. These findings provide new directions for understanding the pathogenesis of HF and developing novel therapies.
摘要：
BACKGROUND: Intervertebral disc degeneration (IDD) significantly contributes to low back pain (LBP), yet effective treatment options are scarce. BSHXF, a classical traditional Chinese medicine formula, demonstrates dual pharmacological actions: tonifying kidneys, strengthening bones, activating blood circulation, and resolving stasis. It has been widely used in IDD management. Given its potential, combining BSHXF with miRNA regulation and stem cell therapy may enhance therapeutic outcomes by targeting molecular and cellular pathways underlying IDD pathogenesis. AIM OF THE STUDY: IDD is recognized as one of the primary causes of low back pain, yet effective therapeutic interventions for this condition remain limited. This study explores the role of BSHXF drug-containing serum combined with adipose-derived stem cells (ADSCs) in slowing IDD progression via the miR-199a-3p/TGF-β/Smad signaling pathway. By comprehensively investigating the synergistic effects of this combination therapy, we aim to propose a novel multi-target strategy that addresses the complex pathogenesis of IDD. MATERIALS AND METHODS: This study employed a combination of in vivo and in vitro models. An IDD model was induced in rat caudal intervertebral discs through needle puncture, while an oxidative stress-induced ADSCs injury model was created in vitro using tert-butyl hydroperoxide (T-BHP). Cell viability was measured with the CCK-8 assay. Cell cycle distribution and mitochondrial reactive oxygen species (ROS) levels were assessed using flow cytometry. Cellular senescence was assessed using SA-β-galactosidase staining. Lactate dehydrogenase (LDH) activity was quantified to evaluate cellular damage. Differentiation into nucleus pulposus-like cells was assessed using immunofluorescence double staining for CD73 and COL2A1. ELISA was used to measure inflammatory cytokines (TNF-α, IL-1β, IL-4, IL-10) in cell supernatants. miR-199a-3p expression was determined using RT-qPCR. Western blotting was employed to quantify COL2A1, SOX9, and ACAN protein levels, reflecting nucleus pulposus-like differentiation and extracellular matrix (ECM) synthesis capacity. Western blotting was employed to assess pathway activity by analyzing the protein expressions of TGF-β1, Smad2, Smad3, and their phosphorylated forms, P-Smad2 and P-Smad3. In vivo experiments assessed histopathological degeneration through hematoxylin-eosin (HE) and Safranin O-Fast Green staining. Immunohistochemistry (IHC) analyzed COL1A1 and COL2A1 expression levels. RT-qPCR quantified miR-199a-3p expression. Western blotting was employed to assess the expression levels of TGF-β1, Smad2, Smad3, P-Smad2, and P-Smad3 for pathway regulation evaluation. RESULTS: Our experimental results demonstrated that serum containing BSHXF significantly alleviated T-BHP-induced oxidative stress, improved the cellular microenvironment, promoted ADSCs proliferation, and decelerated cellular senescence. Further mechanistic analysis revealed that BSHXF significantly activated the TGF-β/Smad signaling pathway, driving the differentiation of ADSCs into nucleus pulposus-like cells and restoring normal cell cycle progression. Overexpression of miR-199a-3p inhibited the TGF-β/Smad pathway, leading to ECM degradation and elevated expression of inflammatory factors (TNF-α, IL-1β). In contrast, BSHXF restored TGF-β/Smad pathway activity by downregulating miR-199a-3p expression. In vivo experiments demonstrated that miR-199a-3p overexpression exacerbated IDD, characterized by reduced COL2A1 expression, elevated COL1A1 levels, and increased disc fibrosis. BSHXF intervention markedly attenuated IDD progression by downregulating miR-199a-3p expression, reducing disc fibrosis, and effectively restoring collagen expression. CONCLUSION: BSHXF activated the TGF-β/Smad pathway to promote the differentiation of ADSCs into nucleus pulposus-like cells. It exerted protective effects by alleviating oxidative stress damage, improving the microenvironment, delaying senescence, and enhancing cellular functions. This study is the first to reveal that miR-199a-3p overexpression exacerbates intervertebral disc fibrosis and degeneration. BSHXF restored TGF-β/Smad pathway activity by downregulating miR-199a-3p expression, thereby improving disc structure and function. This integrated approach offers a novel multi-target intervention strategy for IDD, demonstrating significant therapeutic potential.
期刊：
Frontiers in Medicine,2025年12:1508439 ISSN：2296-858X
通讯作者：
Peng, ZM
作者机构：
[Li, Pengyu] Sch Hunan Univ Tradit Chinese Med, Changsha, Hunan, Peoples R China.;[Zou, Menglong] Hunan Univ Tradit Chinese Med, Hosp 1, Changsha, Hunan, Peoples R China.;[Peng, Ziming; Peng, ZM] Fangchenggang Hosp Tradit Chinese Med, Fangchenggang, Guangxi, Peoples R China.
通讯机构：
[Peng, ZM ] F;Fangchenggang Hosp Tradit Chinese Med, Fangchenggang, Guangxi, Peoples R China.
关键词：
L-shaped association;NHANES;all-cause mortality;chronic diarrhea;serum 25-hydroxyvitamin D
摘要：
BACKGROUND: The aim of this study was to assess the relationship between serum 25-hydroxyvitamin levels and all-cause mortality in patients with chronic diarrhea. METHODS: We carried out a cross-sectional study using information drawn from the National Health and Nutrition Examination Survey (NHANES). To assess mortality outcomes, we compared our data with records from the National Death Index as of December 31, 2011. The NHANES data were used to determine mortality outcome. We used a Cox regression model-based approach to analyze the relationship between serum 25-hydroxyvitamin concentrations and mortality in chronic diarrhea patients. RESULTS: A total of 2,972 participants with chronic diarrhea were included in our study, 488 cases of all-cause mortality were recorded. The study showed an L-shaped relationship between 25-hydroxyvitamin concentrations and all-cause mortality with a threshold of 73.40 nmol/L. On the left side of the threshold, each 1-unit increase in 25-hydroxyvitamin concentrations was associated with a 2.2% reduction in the risk of all-cause mortality (HR 0.978; 95% CI: 0.969, 0.987); however, on the right side of the threshold, there was no significant correlation between 25(OH)D concentrations and all-cause mortality. CONCLUSION: Serum 25-hydroxyvitamin D levels showed an L-shaped association with all-cause mortality in patients with chronic diarrhea, with 73.40 nmol/L as the potential threshold. However, because this was a cross-sectional study, only an association, not a causal relationship, can be inferred. Further prospective studies are needed to confirm these findings and explore the potential impact of vitamin D supplementation on mortality outcomes.
