作者机构：
[陈其华; 王大进] Department of Traditional Chinese Medicine Surgery, The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China;[欧阳政洁; 李博; 赵丹] Graduate School, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China
作者机构：
[周青] Department of Traditional Chinese Medicine Surgery, The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China;[何清湖; 刘朝圣; 宾东华; 陈虹历; 韩忠; 李迎秋] School of Integrated Chinese and Western Medicine, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China
作者机构：
[周兴] Department of Andrology, The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China;[李迎秋] College of Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;[何清湖] Hunan University of Chinese Medicine, Changsha, Hunan 410208, China
通讯机构：
[Qin, Li] H;Hunan Univ Chinese Med, Sch Pharm, 300 Xueshi Rd, Changsha 410208, Hunan, Peoples R China.
关键词：
Long noncoding RNAs;Nuclear receptor;SRA;Transcriptional coactivator
摘要：
Steroid receptor RNA activator (SRA) is a type of long noncoding RNA (lncRNA) which coordinates the functions of various transcription factors, enhances steroid receptor-dependent gene expression, and also serves as a distinct scaffold. The novel, profound and expanded roles of SRA are emerging in critical aspects of coactivation of nuclear receptors (NRs). As a nuclear receptor coactivator, SRA can coactivate androgen receptor (AR), estrogen receptor α (ERα), ERβ, progesterone receptor (PR), glucocorticoid receptor (GR), thyroid hormone receptor and retinoic acid receptor (RAR). Although SRA is one of the least well-understood molecules, increasing studies have revealed that SRA plays a key role in both biological processes, such as myogenesis and steroidogenesis, and pathological changes, including obesity, cardiomyopathy, and tumorigenesis. Furthermore, the SRA-related signaling pathways, such as the mitogen-activated protein kinase (p38 MAPK), Notch and tumor necrosis factor α (TNFα) pathways, play critical roles in the pathogenesis of estrogen-dependent breast cancers. In addition, the most recent data demonstrates that SRA expression may serve as a new prognostic marker in patients with ER-positive breast cancer. Thus, elucidating the molecular mechanisms underlying SRA-mediated functions is important to develop proper novel strategies to target SRA in the diagnosis and treatment of human diseases.
作者机构：
[孟盼; 杜青; 王宇红; 赵洪庆; 徐雅岚] Training Bases, Hunan Key Laboratory of Chinese Materia Medica Powder and Innovative Drugs Established by Provincial and Ministry, Changsha 410208;[王宇红; 杨蕙] The First Hospital of Hunan University of Traditional Chinese Medicine, Changsha 410007, China
作者机构：
[石继连; 贺福元; 贺红; 周逸群; 刘文龙; 杨岩涛] Department of Pharmaceutics, Hunan University of Chinese Medicine, Changsha 410208, China;[石继连; 贺福元; 唐宇; 贺红; 周逸群; 刘文龙; 杨岩涛] Key Laboratory for Traditional Chinese Medicine Property and Pharmacodynamics, State Administration of Chinese Medicine, Changsha 410208, China;[贺福元; 贺红] Modern Traditional Chinese Medicine Preparation Technology and Evaluation Laboratory, Hunan University of Chinese Medicine, Changsha 410208, China;[石继连; 贺福元; 唐宇; 贺红; 周逸群; 刘文龙; 杨岩涛] Supermolecular Mechanism and Mathematic-physics Characterization for Chinese Materia Medica, Hunan University of Chinese Medicine, Changsha 410208, China;[邓凯文] The First Affinity Hospital, Hunan University of Chinese Medicine, Changsha 410007, China
摘要：
Aim To study the relationship between erectile dysfunction and type 2 diabetes mellitus (T2DM)/metabolic syndrome (MetS). Methods This prospective study invited male patients with T2DM attending for a routine outpatient check-up to complete two questionnaires. A general questionnaire was used to collect demographic and clinical characteristics, while sexual function was assessed using the International Index of Erectile Function scoring system. The prevalence of MetS in this patient population was determined using information from the general questionnaire. Risk factors for erectile dysfunction were identified using univariate and multivariate logistic regression analyses. Results A total of 175 patients provided valid questionnaires; of these, 148 (84.6%) had MetS. The prevalence of erectile dysfunction was 90.9% (159/175) in the entire survey population compared with 89.2% (132/148) in patients with MetS. Multivariate logistic regression analysis identified the following risk factors for erectile dysfunction in patients with T2DM and/or MetS: age, blood pressure and duration of diabetes. Conclusion These current findings suggest that the MetS and its components have a negative impact on male erectile function.
期刊：
International Journal of Molecular Sciences,2016年17(3):429 ISSN：1422-0067
通讯作者：
Liao, Duan-Fang
作者机构：
[Du, Ke; Liu, Chan; Chen, Jian-Xiong; Zheng, Xi-Long; Shi, Ya-Ning; Ao, Bao-Xue; Liao, Duan-Fang; Qin, Li] Hunan Univ Chinese Med, Sch Pharm, Changsha 410208, Hunan, Peoples R China.;[Zhu, Neng] Hunan Univ Chinese Med, Hosp 1, Dept Urol, Changsha 410208, Hunan, Peoples R China.;[Chen, Jian-Xiong] Univ Mississippi, Med Ctr, Sch Med, Dept Pharmacol & Toxicol, Jackson, MS 39216 USA.;[Zheng, Xi-Long] Univ Calgary, Libin Cardiovasc Inst Alberta, Dept Biochem & Mol Biol, 3330 Hosp Dr NW, Calgary, AB T2N 4N1, Canada.
通讯机构：
[Liao, Duan-Fang] H;Hunan Univ Chinese Med, Sch Pharm, Changsha 410208, Hunan, Peoples R China.
关键词：
atherosclerosis;caveolae;caveolin-1;inflammation;reverse cholesterol transport
摘要：
Lipid disorder and inflammation play critical roles in the development of atherosclerosis. Reverse cholesterol transport is a key event in lipid metabolism. Caveolae and caveolin-1 are in the center stage of cholesterol transportation and inflammation in macrophages. Here, we propose that reverse cholesterol transport and inflammation in atherosclerosis can be integrated by caveolae and caveolin-1.
摘要：
Ezetimibe, a selective inhibitor of intestinal cholesterol absorption, effectively reduces plasma cholesterol, but its effect on atherosclerosis is unclear. Foam cell formation has been implicated as a key mediator during the development of atherosclerosis. The purpose of this study was to investigate the effects of ezetimibe on foam cell formation and explore the underlying mechanism. Our results showed that ezetimibe reduced atherosclerotic lesions in apolipoprotein E deficient (apoE-/-) mice by lowering cholesterol levels. Treatment of macrophages with Chol:MβCD resulted in foam cell formation, which was concentration-dependently inhibited by the presence of ezetimibe. Mechanically, ezetimibe treatment downregulated the expression of CD36 and scavenger receptor class B1 (SR-B1), but upregulated the expression of apoE and caveolin-1 in macrophage-derived foam cells, which kept consistent with our microarray results. Moreover, treatment with ezetimibe abrogated the increase of phospho-extracellular signal regulated kinase (ERK) 1/2 and their nuclear accumulation in foam cells. Inhibition of the MAPK pathway by the MEK inhibitor PD98059 attenuated the inhibitory effect of ezetimibe on the expression of p-ERK1/2 and caveolin-1. Taken together, our results showed that ezetimibe suppressed foam cell formation via the caveolin-1/MAPK signaling pathway, suggesting that inhibition of foam cell formation might be a novel mechanism underlying the anti-atherosclerotic effect of ezetimibe. This article is protected by copyright. All rights reserved.
摘要：
Objective: The aim of the present study was to investigate the effects of Longdanxiegan formula granule (LDXGFG), a Chinese traditional medicine on Toll-like receptor (TLR) pathway in recurrent genital herpes. Materials and Methods: An experimental recurrent genital herpes model was constructed using herpes guinea pig model. The effect of LDXGFG on expression levels of TLR pathway genes were detected using real-time polymerase chain reaction. Furthermore, the dendritic cells and Langerhans cells were isolated and the TLR pathway genes of these cells were assayed after LDXGFG treatment. Results: The result suggested two different expression patterns of TLR pathway genes in genital herpes and recurrent genital herpes, including upregulated genes and downregulated genes. TLR1, TLR4, TLR6, TLR7, TLR8, TLR9, and TLR10 showed a significant decrease while, TLR2, TLR3, and TLR5 increased in genital herpes and recurrent genital herpes guinea pigs. Meanwhile, the downregulated genes in genital herpes and recurrent genital herpes were stimulated by LDXGFG. By contrast, the upregulated genes decreased significantly after LDXGFG treatment. In both dendritic cells and Langerhans cells, the TLR pathway genes exhibited same pattern: the LDXGFG corrected the abnormal expression of TLR pathway genes. Conclusion: The present results suggest that LDXGFG is an alternative, inexpensive, and lasting-effect medicine for herpes simplex virus 2 infection. Copyright (C) 2016, Taiwan Association of Obstetrics & Gynecology. Published by Elsevier Taiwan LLC.
作者机构：
[田浩梅; 贺平; 颜虹] Key Lab of Correlation between Meridian Acupoints and Viscera of Hunan Administration of Traditional Chinese Medicine, Hunan University of Traditional Chinese Medicine, Changsha 410208, China;[张娟] Ningxiang County Hospital of Traditional Chinese Medicine, Changsha 410600;[陈楚淘] Key Lab of Internal Medicine Constructed by Chinese Ministry of Education and Provincial Government, Hunan University of Traditional Chinese Medicine, Changsha 410007. 314458702@qq.com
作者机构：
[Li, Yingchen; Cheng, Qilai] Hunan Univ Tradit Chinese Med, Postgrad Sch, Changsha 410208, Hunan, Peoples R China.;[Hu, Guoheng] Hunan Univ Tradit Chinese Med, Dept Neurol, Affiliated Hosp 1, Changsha 410007, Hunan, Peoples R China.
通讯机构：
[Hu, Guoheng] H;Hunan Univ Tradit Chinese Med, Dept Neurol, Affiliated Hosp 1, Changsha 410007, Hunan, Peoples R China.
关键词：
human umbilical cord;mesenchymal stem cells;ischemic stroke;cellular therapy;transplantation
摘要：
Ischemic stroke is a focal cerebral insult that often leads to many adverse neurological complications severely affecting the quality of life. The prevalence of stroke is increasing throughout the world, while the efficacy of current pharmacological therapies remains unclear. As a neuroregenerative therapy, the implantation of human umbilical cord mesenchymal stem cells (hUC-MSCs) has shown great possibility to restore function after stroke. This review article provides an update role of hUC-MSCs implantation in the treatment of ischemic stroke. With the unique “immunosuppressive and immunoprivilege” property, hUC-MSCs are advised to be an important candidate for allogeneic cell treatment. Nevertheless, most of the treatments are still at primary stage and not clinically feasible at the current time. Several uncertain problems, such as culture conditions, allograft rejection, and potential tumorigenicity, are the choke points in this cellular therapy. More preclinical researches and clinical studies are needed before hUC-MSCs implantation can be used as a routinely applied clinical therapy.
