作者机构：
Hunan University of Chinese Medicine, Changsha, 410208, China;[李春] Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, China;Hunan Engineering Center for Rapid Test and Removal of Toxic and Harmful Substances in Chinese Medicine, Changsha, 410208, China;[李亚梅; 周亚敏; 皮胜玲; 廖端芳; 林丽美; 柏玉冰; 夏伯候] Hunan University of Chinese Medicine, Changsha, 410208, China, Hunan Engineering Center for Rapid Test and Removal of Toxic and Harmful Substances in Chinese Medicine, Changsha, 410208, China
通讯机构：
[Lin, L.-M.] H;Hunan University of Chinese Medicine, Changsha, China
摘要：
Three new phenolic glycosides 2-(3-O-beta-D-glucopyranosyl-4-hydroxyphenyl) ethanol 1-O-beta-D-glucopyranoside (1), 2-(4-O-beta-D-fructopyranosylphenyl) ethanol 1-O-beta-D-galactopyranoside (2) and 3-methoxy- 4-O-beta-D-allopyranosyl acetophenone (3), along with nine known compounds (4-12), were isolated from the ethanol extract of the whole plant of Aconitum tanguticum (Maxim.) Stapf. Their structures were elucidated by analysis of spectroscopic data including 1D-, 2D-NMR and HRESIMS, and the reported literature data comparison. All the compounds were evaluated for their potential anti-inflammatory effects by the inhibition of TNF-alpha production on LPS-stimulated RAW264.7 macrophages. Compounds 1, 3, 5 and 7-9 showed certain inhibition activity and their IC50 values were 38.18, 27.64, 3.25, 84.45, 12.76 and 18.44 mu g/mL, respectively. (C) 2015 Phytochemical Society of Europe. Published by Elsevier B.V. All rights reserved.
作者机构：
[Zhang Cai-Ping] Univ South China, Coll Med, Hengyang 421001, Peoples R China.;[Lin Li-Mei; Tuo Qin-Hui; Sun Shao-Wei; Gong Yong-Zhen; Liao Duan-Fang] Hunan Univ Chinese Med, State Key Lab Chinese Med Powder & Med Innovat Hu, Div Stem Cell Regulat & Applicat, Changsha 410205, Hunan, Peoples R China.;[Zhang Cai-Ping; Zheng Xing; Sun Shao-Wei; Ou Lu; Lei Xiao-Yong] Univ South China, Coll Pharm & Biol Sci, Hengyang 421001, Peoples R China.;[Tuo Qin-Hui; Gong Yong-Zhen; Liao Duan-Fang] Hunan Univ China, Sinoluxembourg Cooperat Res Ctr Chinese Med, Med, Changsha 410208, Hunan, Peoples R China.
通讯机构：
[Liao Duan-Fang] H;Hunan Univ Chinese Med, State Key Lab Chinese Med Powder & Med Innovat Hu, Div Stem Cell Regulat & Applicat, Changsha 410205, Hunan, Peoples R China.
作者：
Li Chun;Lin Li-Mei;Sui Feng*;Wang Zhi-Min;Huo Hai-Ru;...
期刊：
中国天然药物,2014年12(2):89-102 ISSN：2095-6975
通讯作者：
Sui Feng
作者机构：
[Dai Li; Huo Hai-Ru; Jiang Ting-Liang; Li Chun; Sui Feng; Wang Zhi-Min] China Acad Chinese Med Sci, Inst Chinese Mat Med, Beijing 100700, Peoples R China.;[Lin Li-Mei] Hunan Univ Tradit Chinese Med, Changsha 410208, Peoples R China.
通讯机构：
[Sui Feng] C;China Acad Chinese Med Sci, Inst Chinese Mat Med, Beijing 100700, Peoples R China.
摘要：
Siraitia grosvenorii is a perennial herb endemic to Guangxi province of China. Its fruit, commonly known as Luo hanguo, and has been used for hundreds of years as a natural sweetener and as a traditional medicine for the treatment of pharyngitis, pharyngeal pain, as well as an anti-tussive remedy in China. Based on ninety-three literary sources, this review summarized the advances in chemistry, biological effects, and toxicity research of S. grosvenorii during the past 30 years. Several different classes of compounds have been isolated or detected from various parts of S. grosvenorii, mainly triterpenoids, flavonoids, polysaccharides, amino acids, and essential oils. Various types of extracts or individual compounds derived from this species exhibited a wide array of biological effects e.g. anti-tussive, phlegm-relieving, anti-oxidant, immunomodulatory, liver-protecting, glucose-lowering, and anti-microbial. The existing research has shown that extracts and individual compounds from S. grosvenorii are basically non-toxic. Finally, some suggestions for further research on specific chemical and pharmacological properties of S. grosvenorii are proposed in this review.
摘要：
目的:研究10-羟基喜树碱对肝癌细胞HepG2的DNA甲基化水平的调节作用。方法:体外培养HepG2细胞,分为给药组(10-羟基喜树碱25 μg/ml)和空白对照组,每组6个小组,每小组5个平行孔,分别于给药后24、48、72 h 各组取2个小组,一组用MTT法检测HepG2细胞的生长抑制率;另一组提取HepG2细胞的DNA,酸水解后高效液相色谱法检测其甲基化率。结果:10-羟基喜树碱作用24、48、72 h 后,HepG2细胞的生长抑制率分别为(61.6±4.9)%、(85.7±0.7)%、(97.9±0.7)%;给药组HepG2细胞DNA 甲基化率分别为(2.81±0.34)%、(6.67±0.24)%、(6.83±0.24)%,明显高于相应的空白对照组[(1.88±0.13)%、(1.91±0.11)%、(1.98±0.18)%](P<0.05),且与作用时间呈正相关。结论:10-羟基喜树碱在抑制HepG2细胞的生长的同时提高了细胞DNA的甲基化水平。
