期刊：
Frontiers in Genetics,2020年11:591079 ISSN：1664-8021
通讯作者：
Luo, GQ
作者机构：
[Luo, Guoqing; Zhuang, Gaojian] Guangzhou Med Univ, Qingyuan Peoples Hosp, Affiliated Hosp 6, Qingyuan, Peoples R China.;[Zeng, Yu] Tianjin Med Univ Canc Inst & Hosp, Key Lab Canc Prevent & Therapy, Dept Thyroid & Neck Tumor, Natl Clin Res Ctr Canc, Tianjin, Peoples R China.;[Tang, Qun] Hunan Univ Chinese Med, Dept Pathol, Changsha, Peoples R China.;[He, Qian] Jinan Univ, Affiliated Hosp 1, Dept Neurosurg, Guangzhou, Peoples R China.
通讯机构：
[Luo, GQ ] G;Guangzhou Med Univ, Qingyuan Peoples Hosp, Affiliated Hosp 6, Qingyuan, Peoples R China.
关键词：
M1 macrophages;CIBERSORT;weighted gene co-expression network analysis (WGCNA);nomogram;thyroid cancer (THCA)
摘要：
Macrophages are key innate immune cells in the tumor microenvironment that regulate primary tumor growth, vascularization, metastatic spread and response to therapies. Macrophages can polarize into two different states (M1 and M2) with distinct phenotypes and functions. To investigate the known tumoricidal effects of M1 macrophages, we obtained RNA expression profiles and clinical data from The Cancer Genome Atlas Thyroid Cancer (TCGA-THCA). The proportions of immune cells in tumor samples were assessed using CIBERSORT, and weighted gene co-expression network analysis (WGCNA) was used to identify M1 macrophage-related modules. Univariate Cox analysis and LASSO-Cox regression analysis were performed, and four genes (SPP1, DHRS3, SLC11A1, and CFB) with significant differential expression were selected through GEPIA. These four genes can be considered hub genes. The four-gene risk-scoring model may be an independent prognostic factor for THCA patients. The validation cohort and the entire cohort confirmed the results. Univariate and multivariate Cox analysis was performed to identify independent prognostic factors for THCA. Finally, a prognostic nomogram was built based on the entire cohort, and the nomogram combining the risk score and clinical prognostic factors was superior to the nomogram with individual clinical prognostic factors in predicting overall survival. Time-dependent ROC curves and DCA confirmed that the combined nomogram is useful. Gene set enrichment analysis (GSEA) was used to elucidate the potential molecular functions of the high-risk group. Our study identified four genes associated with M1 macrophages and established a prognostic nomogram that predicts overall survival for patients with THCA, which may help determine clinical treatment options for different patients.
摘要：
目的:探讨健脾益气利水方(J P F)对脑缺血大鼠脑水肿及脑组织紧密连接蛋白闭合蛋白(Occludin)、密封蛋白-1(Claudin-1)、闭锁连接蛋白-1(ZO-1)表达的影响。方法:将105只雄性SD大鼠随机分为假手术组,模型组,依达拉奉组,JPF小、中、大剂量组。采用线栓法制备大脑中动脉栓塞(MCAO)模型,治疗7d后处死,运用伊文思蓝(EB)渗出法检测血脑屏障的通透性,运用免疫组化法检测大鼠缺血侧脑组织Occludin、Claudin-1、ZO-1蛋白表达。结果:与假手术组比较,模型组缺血侧脑组织EB的含量显著增加(P<0.01),Occludin、Claudin-1、ZO-1阳性细胞表达显著降低(P<0.01);与模型组比较,依达拉奉组和JPF中、大剂量组大鼠缺血侧脑组织EB的含量明显降低(P<0.05),各给药组Occludin、Claudin-1、ZO-1阳性细胞表达明显增强(P<0.01,P<0.05)。结论:健脾益气利水法组方可能通过上调紧密连接蛋白Occludin、Claudin-1、 ZO-1表达保护血脑屏障,减轻脑缺血再灌注大鼠脑水肿。
作者机构：
[Mei-hua, Yang; Chun-yu, Li; Dan, Gao] Chinese Acad Med Sci, Inst Med Plant Dev, Beijing 100193, Peoples R China.;[Mei-hua, Yang; Chun-yu, Li; Dan, Gao] Peking Union Med Coll, Beijing 100193, Peoples R China.;[Xiao-he, Xiao; Jia-bo, Wang; Yun, Zhu; Rui-yu, Li; Wu-wen, Feng; Chun-yu, Li; Dan, Gao; Qin, He] Chinese Med 302 Mil Hosp, China Mil Inst, Beijing 100039, Peoples R China.;[Qin, He] Hunan Univ Tradit Chinese Med, Sch Pharm, Changsha 410208, Hunan, Peoples R China.;[Hui-fang, Li] Shanxi Univ Tradit Chinese Med, Sch Pharm, Chengdu 611137, Sichuan, Peoples R China.
通讯机构：
[Jia-Bo, W ] C;Chinese Med 302 Mil Hosp, China Mil Inst, Beijing 100039, Peoples R China.
摘要：
The use of Chinese medicinal herbs (CMH) continues to expand rapidly across the world with a deep belief that herbs are safe because they are "natural". However, a survey of the literature indicates that CMH related hepatotoxicity is increasing and has been a worldwide problem. Polygonum multiflorum Thunb. (P. multiflorum), officially listed in the Pharmacopoeia of the People's Republic of China, has been used effectively for thousands of years in China as a popular herb to prevention aging and hair graying and it is generally regarded as non-toxic. However, a number of worldwide reports regarding adverse reactions to P. multiflorum, especially liver injury, has increased in recent years. For this reason, the herbs' negative benefit/risk profile has raised objections about its efficiency even if the use of CMH to treat various diseases with a long history. Furthermore, many liver injury reports appear rarely conclusive, even if which have some levels of causality proof.
摘要：
缺血性脑卒中是脑血管和血液循环发生障碍所引起的中枢神经系统急性疾病,又称脑梗死[1](cerebral infarction, CI ),是指局部脑组织因血液循环障碍,缺血、缺氧而发生的软化坏死.脑卒中一证,是严重危害人类健康的常见病、多发病,以突然昏仆,半身不遂,口舌歪斜,言语蹇涩或不语,偏身麻木为主症,发病急骤,变化迅速,而且致死、致残率高,对于该病的治疗仍是临床上一个难题,中医药治疗本病具有广阔的前景,发挥了一定作用[2],而中医活血化瘀法对临床治疗及预防脑卒中病具有重要意义,现综述如下.
