作者机构：
[Kun Ai; Yan-ling Li; Fang Qi; Xiao-rong Chang] Hunan University of Chinese Medicine, Changsha, China;[刘梨; Zhao-an Yu] The First Hospital of Hunan University of Chinese Medicine, Changsha, China
通讯机构：
[Xiao-rong Chang] H;[Zhao-an Yu] T;Hunan University of Chinese Medicine, Changsha, China<&wdkj&>The First Hospital of Hunan University of Chinese Medicine, Changsha, China
关键词：
Acupuncture Therapy;Electroacupuncture;Arthritis, Rheumatoid;Arthritis, Experimental;Inhibitor of Apoptosis Proteins;Rats
作者机构：
[Yongmei PENG; Ming XU; Guilan HUANG; Hong ZHANG; Kun AI] College of Acupuncture, Moxibustion and Tuina, Hunan University of Chinese Medicine, Changsha 410208, China (湖南中医药大学针灸推拿学院, 长沙 410208, 中国)
通讯机构：
[Hong ZHANG] C;College of Acupuncture, Moxibustion and Tuina, Hunan University of Chinese Medicine, Changsha 410208, China (湖南中医药大学针灸推拿学院, 长沙 410208, 中国)
关键词：
电针;骶上脊髓损伤;神经原性膀胱;尿流动力学
摘要：
Objective:To investigate the effect and mechanism of electroacupuncture (EA) in the intervention of neurogenic bladder (NB) caused by suprasacral spinal cord injury (SCI). Methods:The total 60 female Sprague-Dawley (SD) rats were divided into blank group, sham operation group by adopting random number table method, with 12 rats in each group. The remaining rats were made into models through operation, and 24 successful model rats were randomly divided into model group and EA group, with 12 rats in each group. Since the 19th day after modeling, EA was conducted at "Ciliao"(次髎BL 32), "Zhongji" (中极CV 3), "Sanyinjiao"(三阴交SP 6) and "Dazhui"(大椎GV 14) in EA group, remaining the needle for 20 min every time. The intervention was performed for once a day, and 7 consecutive days were needed. The rest of the three groups were only tied up for the same time. Uro- dynamics test was conducted after the intervention. The contents of PACAP-38 and PAC1R of the spinal cord of the injury site were detected by Western blot. Results:Compared with the blank group, there were no significant differences in the bladder capacity, leak point pressure, bladder compliance and PACAP-38 and PAC1R contents in spinal cord in sham operation group; compared with the sham operation group, the maximum bladder capacity and the bladder compliance in the model group were lower (both P<0.05), the leak point pressure was higher (P<0.05), the contents of PACAP-38 and PAC1R were lower (both P<0.05); compared with the model group, the maximum bladder capacity and the bladder compliance in the EA group were higher (both P<0.05), the leak point pressure was lower (P<0.05), the contents of PACAP-38 and PAC1R were higher (both P<0.05). Conclusion:EA at BL 32, CV 3, SP 6 and GV 14 can improve the bladder function of NB rats of suprasacral SCI, and its mechanism may be related to the increase expression of PACAP-38 and PAC1R protein in the injured spinal cord tissue.
摘要：
Objective: To observe the effect of An-pressing manipulation on biceps brachii with delayed onset muscle soreness (DOMS) in healthy male volunteers. Methods: A total of 30 male college student volunteers were randomly divided into a blank group, a model group and a treatment group, 10 cases in each group. Subjects in the blank group did not receive any intervention; subjects in the model group received active weight-bearing eccentric exercise on the non-favored side of the upper limb to establish the models, while not receiving any treatment; subjects in the treatment group received both the same modeling and An-pressing manipulation treatment. The subjective rating of perceived exertion (RPE), subjective soreness sensation threshold and soreness grade were evaluated before modeling, immediately after modeling, and 24, 48, 72, 96 and 120 h after modeling. Serum total antioxidant capacity (T-AOC) was measured before modeling, immediately after modeling, and 24, 48 and 72 h after modeling. Serum creatine kinase MM isoenzyme (CK-MM) was measured before modeling and 24, 48 and 72 h after modeling. Results: At 24, 48, 72 and 120 h after treatment, the soreness grades of the treatment group were lower than those of the model group (all P<0.05). The RPE scores of the treatment group were lower than those of the model group (all P<0.05) immediately after modeling, at 24, 48, 72, 96 and 120 h after modeling. The subjective soreness sensation threshold of the treatment group was higher than that of the model group immediately after modeling, at 24, 48, 72 and 96 h after modeling (all P<0.05). Immediately after modeling, T-AOC value in the treatment group was higher than that in the model group and blank group (both P<0.05). CK-MM of the treatment group was lower than that of the model group at 48 h and 72 h after modeling (P<0.05). Conclusion: An-pressing manipulation shows a certain therapeutic effect on biceps brachii with DOMS by strengthening the body's antioxidant and anti-damage abilities, which can effectively reduce the pain and accelerate the recovery from fatigue damage.
摘要：
Objective:To observe the influence of pressing force and time on the thermal effect of An-pressing manipulation.Methods:Eight healthy volunteers were recruited to receive An-pressing manipulation at Xinshu (BL 15) on the right side.The pressing force and time were both divided into five levels:the force described as extremely mild,mild,moderate,strong and extremely strong and time given by 2.5 min,5.0 min,7.5 min,10.0 min and 15.0 min.The real-time change in local acupoint temperature as well as the change during 1.0-15.0 min after the manipulation were observed.Results:Compared with the baseline data,the real-time changes in the temperature after An-pressing Xinshu (BL 15) on the right side with different levels of force (from mild to strong) were respectively (1.88t0.64) ℃,(2.05±0.68) ℃,(2.25±0.59) ℃,(2.35±0.61) ℃ and (2.32±0.69) ℃;the changes in 15.0 min after the manipulation were respectively (-0.11±0.11) ℃,(0.03±0.14) ℃,(0.59±0.58) ℃,(1.38±0.70) ℃ and (2.09±0.98) ℃.The real-time temperature changes after the manipulation for different durations (from short to long) were respectively (1.94±0.37) ℃,(2.33±0.29) ℃,(2.49±0.31) ℃,(2.51±0.39) ℃ and (2.41±0.55) ℃;the changes in 15.0 min after the manipulation were respectively (0.53±0.49) ℃,(0.33±0.30) ℃,(0.52±0.33) ℃,(0.55±0.38) ℃ and (0.76±0.36) ℃.Conclusion:The thermal effect presented an increasing tendency with the extension of pressing time,and the temperature reached the top at 7.5 min;the thermal effect showed an increasing tendency with the rise of pressing force,and the temperature reached the top upon a moderate level of force.The pressing time can produce a greater influence on the real-time temperature than the pressing force;the pressing force can produce a greater influence on maintaining the temperature than the pressing time.
