作者机构：
[Ming XU; Hong ZHANG; Xiu-ting YIN; Ji-sheng LIU; Jian ZHANG; Yong-mei PENG; Shi-feng DENG; Xi-qin YI; Fang QI; Kun AI] College of Acupuncture-Moxibustion and Tuina, Hunan University of Chinese Medicine, Changsha 410208, Hunan Province, China
摘要：
To observe the effect of electroacupuncture on the morphological change of the bladder tissue and the protein expression levels of cytochrome C, Caspase-3 and Caspase-9 in the bladder tissue of rats with neurogenic bladder after suprasacral spinal cord injury, and to preliminarily explore its partial mechanism of action.
Forty-eight female SD rats were randomly divided into blank group (group B), sham operation group (group S), model group (group M) and electroacupuncture group (group EA) according to random number table method with 12 rats in each group. Neurogenic bladder models after suprasacral spinal cord injury were established by adopting modified spinal cord transection method. Electroacupuncture intervention was conducted at “Cìliáo (
suprasacral spinal cord injury can increase the protein expression levels of cytochrome C, activated Caspase-3 and Caspase-9 in bladder ti ssues of rats, and electroacupuncture at BL 32, CV 3, SP 6 and GV 14 can down-regulate the protein expression levels of cytochrome C, acti vated Caspase-3 and Caspase-9 in bladder ti ssues of rats aft er suprasacral spinal cord injury and protect bladder ti ssue, which may be one of the mechanisms that electroacupuncture protects the involved bladder detrusor aft er suprasacral spinal cord injury.
作者机构：
[Kun AI; Jin-feng WU; Fang QI; Hong ZHANG; Xi-qin YI; Xi LING] College of Acupuncture & Moxibustion and Tuina, Hunan University of CM, Changsha 410208, Hunan Province, China
关键词：
电针;下合穴;AGML模型大鼠;合治内府
摘要：
Objective To contrastively observe the effect of electroacupuncture at lower he-sea points of stomach, large intestine, small intestine and gallbladder of acute gastric mucosal lesion (AGML) model rats on the expression of serum high mobility group protein B 1 (HMGB 1), tumor necrosis factor-α (TNF-α) and nuclear factor-κB (NF-κB) of gastric tissue, and to explore whether the lower he-sea points of stomach have relative specificity in treatment of stomach diseases. Methods Seventy healthy SD rats were randomly divided into seven groups, including control group, model group, “Zusanli” (足三里ST 36) group, “Shangjuxu” (上巨虚ST 37) group, “Xiajuxu” (下巨虚ST 39) group, “Yanglingquan” (阳陵泉GB 34) group and “Chengjin” (承筋BL 56) group, with five male rats and five female rats in each group. Acute gastric mucosal lesion model was established by applying WRS method. After successful modeling and treatment for ten days, sampling was conducted, gastric mucosal lesion was observed visually and scored, the levels of serum HMGB 1 and gastric tissue TNF-α were detected by ELISA method, and the expression of NF-κB was detected by immunohistochemical method. Results ① Compared with the model group, the levels of Ulcers and inflammation (UI) in gastric mucosal and serum HMGB1, and the expression of gastric tissue TNF-α and NF-κB in the four lower he-sea points groups reduced (P<0.01 or P<0.05); ② compared with ST 36 group, the levels of gastric mucosal UI and serum HMGB1, and the expression of gastric mucosal TNF-α and NF-κB in other treatment groups increased significantly, and the difference was statistically significant (P<0.01 or P<0.05). Conclusion ① Electroacupuncture at relevant lower he-sea points can inhibit the body’s immune response, relieve inflammatory reaction and reduce mucosal lesion by regulating HMGB 1, TNF-α and NF-κB, so as to realize the intervention and treatment on AGML rats; ② the total intervention effect in ST 36 group is superior to that in other groups, indicating partially that there is a relative specificity between ST 36 and stomach.
