作者机构：
[丁煌; 唐三; 杨筱倩; 邓常清; 黄小平; 刘晓丹] Molecular Pathology Lab, Key Lab of Hunan Province for Prevention and Treatment of Integrated Traditional Chinese and Western Medicine on Cardio-cerebral Diseases, Key Lab of Hunan Universities for Cell Biology and Molecular Techniques, Hunan University of Chinese Medicine, Changsha, 410208, China
摘要：
<jats:p>Although the compatibility of Astragali Radix (AR) and Angelicae Sinensis Radix (ASR) has favorable effect on promoting hematopoiesis in traditional Chinese medicine (TCM), the main active components and pharmacological mechanism are unknown. We investigated the five active components and its mechanisms <jats:italic>in vitro</jats:italic> and <jats:italic>in vivo</jats:italic>. Five active components of Astragalus glycosides (AST), Formononetin (FRM), Ferulic acid (FRA), Calycosin (CAL), and Calycosin-7-glucoside (CLG), which could be absorbed in intestinal tract, were detected in this study. The peripheral blood, hematopoietic growth factors (HGFs), and hematopoietic progenitor cells (HPCs) colony were observed to evaluate the effect of these five active components promoting hematopoiesis. Furthermore, hematopoietic stem cell (HSC) proliferation, aging, cycle, and related proteins were detected to explore the mechanism of these five components promoting HSC proliferation. <jats:bold>i)</jats:bold> The <jats:italic>in vivo</jats:italic> experiments showed that the combination of the five active components could remarkably increase the number of RBCs, WBCs, PLTs, and content of Hb in peripheral blood and the area of bone marrow hematopoietic tissue, as well as thrombopoietin (TPO), erythropoietin (EPO), granulocyte-macrophage colony stimulating factor (GM-CSF), and colony of CFU-GM, CFU-MK, CFU-E, and BFU-E in serum. Each of these five components promoted the recovery of RBCs and Hb, and increased TPO, CFU-MK, and CFU-E. All components except for AST increased the CFU-GM. FRA increased the number of WBCs, the area of bone marrow hematopoietic tissue, and BFU-E. FRA and AST promoted PLT recovery. FRA and CAL improved the content of GM-CSF. FRA, CAL, and CLG improved the content of EPO. <jats:bold>ii)</jats:bold> The <jats:italic>in vitro</jats:italic> experiments showed that FRA, FRM, and AST significantly promoted cell proliferation, reduced the positive rate and G0/G1 cells, and increased G2/M + S cells and the expression of cyclin D1 and CDK4 proteins in aging HSCs. Furthermore, the combination of five components had the best effect. Taken together, the five active components of AST, FRM, FRA, CAL, and CLG were the main pharmacodynamic substances of the AR-ASR compatibility, which promoted hematopoiesis. The combination of them had a synergistic effect. The mechanism of promoting hematopoiesis may be relevant to regulating cyclin-related proteins, promoting cell cycle transformation, and promoting HSC proliferation.</jats:p>
作者机构：
[丁煌; 邓常清; 刘晓丹] Molecular Pathology Laboratory, Hunan Key Laboratory of Cerebrovascular Disease Prevention and Treatment of Integrated Traditional Chinese and Western Medicine, Key Laboratory of Hunan University for Cell Biology and Molecular Techniques, Hunan University of Chinese Medicine, Changsha, 410208, China
通讯机构：
[Deng, C.-Q.] M;Molecular Pathology Laboratory, Hunan Key Laboratory of Cerebrovascular Disease Prevention and Treatment of Integrated Traditional Chinese and Western Medicine, Key Laboratory of Hunan University for Cell Biology and Molecular Techniques, Hunan University of Chinese Medicine, Changsha, China
通讯机构：
[Huang, X.-P.] K;Key Laboratory of Hunan Province for Prevention and Treatment of Integrated Traditional Chinese and Western Medicine on Cardio-Cerebral Diseases, China
作者机构：
[Shao-wu Cheng; Huai-gang Qu; Xiao-dan Liu; Zhen-yan Song; Ping Li; Hong-wen He; Jin-wen Ge] College of integrated Traditional Chinese Medicine and Western Medicine,Hunan University of Chinese Medicine,Key Laboratory of Hunan Province for Integrated Traditional Chinese and Western Medicine on Prevention and Treatment of Cardio-Cerebral Diseases;[Shao-wu Cheng; Huai-gang Qu; Xiao-dan Liu; Zhen-yan Song; Ping Li; Hong-wen He; Jin-wen Ge] Department of Anatomy,Zhongshan School of Medicine,Sun Yat-sen University;[Shao-wu Cheng; Huai-gang Qu; Xiao-dan Liu; Zhen-yan Song; Ping Li; Hong-wen He; Jin-wen Ge] Guanghua School of Stomatology,Sun Yat-sen University,Guangdong Provincial Key Laboratory of Stomatology
会议名称：
第六届亚太神经科学联合会学术会议暨中国神经科学学会第十一届全国学术会议
会议时间：
2015-09-20
会议地点：
中国浙江桐乡
摘要：
Danggui-Shaoyao-San(DSS),a traditional Chinese medicinal prescription,could alleviate cognitive dysfunction of Alzheimer’s disease(AD) patients.However,the detail mechanisms remain elusive.In the pres
作者机构：
[DING Huang; LI Jing-xian; YANG Xiao-qian; TANG Biao; LIU Xiao-dan; TANG Ying-hong; DENG Chang-qing; HUANG Xiao-ping] Molecular Pathology Laboratory,Key Laboratory of Hunan Province for Prevention and Treatment of Integrated Traditional Chinese and Western Medicine on Cardio-Cerebral Diseases,Key Laboratory of Hunan Universities for Cell Biology and Molecular Techniques,Hunan University of Chinese Medicine
会议名称：
2016年国际生理学学术大会
会议时间：
2016-09-25
会议地点：
中国北京
摘要：
AIM: To probe the effects and the mechanism of Astragaloside IV and Ginsenoside Rg1 on autophagy of PC12 cell induced by oxygen glucose deprivation/reoxygenation(OGD/R).METHODS: The autophagy injury m
