作者机构：
[彭艳; 刘丽; 刘密; 吴雪芬; 郭鑫; 岳增辉; 郑雪娜] College of Acupuncture and Moxibustion, Hunan University of Chinese Medicine, Changsha 410007, China;[袁建菱] College of Acupuncture and Moxibustion, Hunan University of Chinese Medicine, Changsha 410007, China. 624755064@qq.com
作者机构：
[谢文娟; 刘密; 林海波; 谭成富; 贺香嫦; 常小荣; 阳晶晶] College of Acu-moxibustion and Massage, Hunan University of Traditional Chinese Medicine, Changsha 410007, China;[严洁] College of Acu-moxibustion and Massage, Hunan University of Traditional Chinese Medicine, Changsha 410007, China. yj5381159@126.com;[王超] College of Acu-moxibustion and Massage, Hunan University of Traditional Chinese Medicine, Changsha 410007, China. 592436380@qq.com
作者机构：
[杨建文; 刘丽; 刘薇薇; 陈海交; 张程程] Graduate School, Hunan University of Traditional Chinese Medicine, Changsha 410208, China;[彭艳; 刘密] Key Lab for Acu-moxibustion Biological Information Analysis of College of Acu-moxibustion and Massage, Hunan University of Traditional Chinese Medicine, Changsha 410208, China;[林亚平] Key Lab for Acu-moxibustion Biological Information Analysis of College of Acu-moxibustion and Massage, Hunan University of Traditional Chinese Medicine, Changsha 410208, China. lyp5381161@126.com
作者机构：
[Qiong LIU; Mai-lan LIU; Hao LIANG; Mi LIU; Xiao-rong CHANG] College of Acupuncture & Moxibustion and Tuina, Hunan University of Chinese Medicine, Changsha 410208, Hunan Province, China;[Tian-ai SUN; Huan-gan WU] Shanghai Research Institute of Acupuncture and Meridian, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;[Jia-feng DING; Xue-mei XU] School of Physical Science and Electronics, Central South University, Shanghai 201203, China;School of Business, Central South University, Shanghai 201203, China;[Guo-bin DAI] College of Acupuncture & Moxibustion and Tuina, Hunan University of Chinese Medicine, Changsha 410208, Hunan Province, China<&wdkj&>School of Business, Central South University, Shanghai 201203, China
关键词：
Mild moxibustion;distance;receptor temperature;temperature sensor;photothermal detection system for moxibustion combustion
摘要：
Objective To explore the relationship between the receptor temperature and moxibustion distance of mild moxibustion, and provide evidence for clinical moxibustion distance. Methods Mild moxibustion was carried out for 15min by adopting moxibustion shelf, and the moxibustion ash was shook offautomatically for once every 3 min. The distance between moxa stick and temperature sensor was set as 2 cm, 3 cm, 4 cm and 5 cm, respectively, by ruler. The temperature was recorded for once every 2 s with photothermal detection system for moxibustion combustion to form a temperature profile. The initial temperature, maximum temperature and average temperature were observed, and the experimental data were analyzed via SPSS18.0 statistical software. Results The initial receptor temperature was (25.33±0.29) ℃ when the moxibustion distance was 2 cm, (25.29±0.05) ℃ when the moxibustion distance was 3 cm, (25.43±0.17) ℃ when the moxibustion distance was 4cm, and (25.16±0.22) ℃ when the moxibustion distance was 5 cm, respectively. The maximum temperature was (74.96±1.20) ℃ when the moxibustion distance was 2 cm, (51.70±0.74) ℃ when the moxibustion distance was 3 cm, (49.33±0.40) ℃ when the moxibustion distance was 4 cm, and (42.50±0.26) ℃ when the moxibustion distance was 5 cm, respectively. The average temperature was (62.40±7.84) ℃–(62.68±7.58) ℃ when the moxibustion distance was 2 cm, (44.77±3.31) ℃–(45.11±3.21) ℃ when the moxibustion distance was 3 cm, (42.72±3.86) ℃–(43.12±3.54) ℃ when the moxibustion distance was 4 cm, and (38.45±1.67) ℃–(38.50±1.63) ℃ when the moxibustion distance was 5 cm, respectively. The temperature curve showed that the fluctuation range was the maximum when the moxibustion distance was 2 cm, larger when the moxibustion distance was 3 cm, smaller when the moxibustion distance was 4 cm, and minimum when the moxibustion distance was 5 cm. There was no significant difference in the initial temperature of mild moxibustion at different distances, however, the maximum temperature, average temperature and the fluctuation range of temperature curve were closely related to the moxibustion distance. Conclusion The nearer the distance is, the higher the maximum temperature and average temperature as well as the larger the fluctuation range will be, and the farther the distance is, the lower the maximum temperature and average temperature as well as the smaller the fluctuation range will be. According to preliminary analysis, the temperature will be the most suitable when the moxibustion distance is 3 cm–4 cm.
作者机构：
[陈海交; 林亚平; 杨建文; 张程程; 彭艳; 刘薇薇; 刘丽; 刘密; 岳增辉] Hunan University of Chinese Medicine, Changsha, 410208
通讯机构：
[Yan Peng] H;Hunan University of Chinese Medicine, Changsha, China
关键词：
Acupuncture Therapy;Electroacupuncture;Specificity of Acupoints;Point Selection;Diabetes Complications;Gastroparesis;Rats
摘要：
Objective: To observe the effect of electroacupuncture (EA) on the electrogastrogram and gastric antrum ghrelin in rats with diabetic gastroparesis (DGP). Methods: Fifty Sprague-Dawley (SD) rats were randomly divided into group A, group B, group C, group D and group E, 10 rats in each group. Group A was the blank control group without intervention. Group B, Group C, Group D and Group E were treated with single dose intraperitoneal injection of 2% streptozotocin (STZ), combined with 8-week high glucose and high fat diet to establish DGP rat models. Group B was the model group without treatment. Group C was the EA at acupoint group, was treated with EA at Zusanli (ST 36), Liangmen (ST 21) and Sanyinjiao (SP 6). Group D was the EA at non-acupoint group, was treated by EA at the control points of Zusanli (ST 36), Liangmen (ST 21) and Sanyinjiao (SP 6). Rats in the metoclopramide control group received 1.7% metoclopramide solution [10 mL/(kg·bw)] by gavage. Rat's blood glucose level was measured by blood glucose meter; gastric emptying rate was detected using phenol red as a marker; the electrogastrogram was detected by BL-420F biological function system; the protein level of ghrelin was detected by enzyme-linked immunosorbent assay (ELISA); the expression of ghrelin mRNA was detected by real-time polymerase chain reaction (RT-PCR). Results: Compared with group A, the blood glucose of group B, C, D and E were significantly increased before and after the treatment (all P<0.01); after treatment, the gastric emptying rate of group B was significantly decreased (P<0.01), the migration rates of small intestine in group B, C, D and E were significantly lower (all P<0.01), and the protein content of ghrelin in group C was significantly decreased (P<0.01); the expressions of ghrelin mRNA were significantly increased in group B, C, D and E (all P<0.01), the mean amplitudes of electrogastrogram in group B and D were significantly decreased (both P<0.01). After treatment, compared with group B, the blood glucose of group C was significantly decreased (P<0.05), the gastric emptying rate and small intestine migration rate were significantly increased in group C and E (P<0.05, P<0.01), the small intestinal migration rate was significantly increased in group D (P<0.05), the expression of ghrelin in protein and mRNA in group C was significantly lower (P<0.01), the expression of ghrelin mRNA in group E was significantly lower (P<0.05), and the mean amplitude of electrogastrogram in group C was significantly increased (P<0.05). After treatment, compared with group D, the protein and mRNA expressions of ghrelin in group C were significantly decreased (P<0.01). After treatment, compared with group E, the protein expression of ghrelin in group C was significantly decreased (P<0.01). Conclusion: EA at Zusanli (ST 36), Liangmen (ST 21) and Sanyinjiao (SP 6) could regulate the blood glucose level of DGP model rats, enhance electrogastrogram activity, promote gastric emptying, and regulate ghrelin expression in protein and mRNA.
作者机构：
School of Acupuncture, Moxibustion & Tuina, Hunan University of Chinese Medicine, Changsha, 410208;Shanghai Research Institute of Acupuncture and Meridian, Shanghai University of Traditional Chinese Medicine, Shanghai, 200030;[刘琼; 梁昊; 贲定严; 刘迈兰; 常小荣; 刘密] School of Acupuncture, Moxibustion & Tuina, Hunan University of Chinese Medicine, Changsha, 410208;[孙天爱; 刘慧荣; 吴焕淦] Shanghai Research Institute of Acupuncture and Meridian, Shanghai University of Traditional Chinese Medicine, Shanghai, 200030
通讯机构：
[Xiao-rong Chang; Mai-lan Liu] S;School of Acupuncture, Moxibustion & Tuina, Hunan University of Chinese Medicine, Changsha, China<&wdkj&>School of Acupuncture, Moxibustion & Tuina, Hunan University of Chinese Medicine, Changsha, China
摘要：
Objective: To explore the correlation between moxibustion sensation and distance of moxa stick and provide reference for clinical practice. Methods: A total of 16 healthy volunteers aged 18-35 years old in college were recruited and given mild moxibustion at Shousanli (LI 10), Zusanli (ST 36), Shenshu (BL 23) and Tianshu (ST 25) with moxa stick, and the occurrence and frequency of moxibustion sensation were recorded at distances of 5 cm, 4 cm, 3 cm and 2 cm. Mild moxibustion scale was used to count the score. Results: Warm was the main moxibustion sensation, burning pain and soreness decreased with the rise of distance; for the same acupoint, score of mild moxibustion scale increased with the decrease of distance; score ranged between 5.5 and 6.5 at distance 3 cm, which was the most comfortable distance for volunteers. Conclusion: The distance of 3 cm is the most comfortable distance in mild moxibustion.
摘要：
目的对比观察艾灸预处理和电针预处理两种不同方法对急性胃黏膜损伤大鼠转化生长因子（TGF-α）和增殖细胞核抗原（PCNA）表达的影响差异。方法将40只SPF级的SD大鼠随机的分为4组（空白组、模型组、艾灸预处理组和电针预处理组）,艾灸预处理组和电针预处理组选取"足三里穴"、"中脘穴"分别行艾灸和电针预处理8 d后,除空白组外造模,选用无水乙醇灌胃的方法（0.5 m L/100 g）制成大鼠急性胃黏膜损伤模型。造模1 h后,用20%乌拉坦（0.6 m L/100 g）进行腹腔注射麻醉,再取材。在光镜下观察大鼠的胃黏膜组织形态学的改变,免疫组化法检测胃黏膜TGF-α、PCNA的表达。结果与空白组相比较,模型组胃黏膜中TGF-α、PCNA的表达明显升高（P<0.05或P<0.01）;与模型组相比较,艾灸预处理组和电针预处理组胃黏膜中TGF-α、PCNA的表达均有不同程度升高（P<0.05或P<0.01）;与艾灸预处理组相比较,电针预处理组胃黏膜中TGF-α的表达明显降低,具有统计学意义（P<0.05或P<0.01）,而胃黏膜中PCNA的表达则无明显差异（P>0.05）。结论艾灸预处理与电针预处理均可以减轻无水乙醇对胃黏膜造成的损伤,促进胃黏膜保护因子（TGF-α、PCNA）的表达,但艾灸预处理稍优于电针预处理。
作者机构：
[陈慧娟; 杨宗保; 王亚东] Department of TCM, Medical College of Xiamen University, Xiamen 361005, Fujian Province, China;[常小荣; 刘密; 刘琼] College of Acupuncture-Moxibustion and Tuina, Hunan University of CM, Changsha 410208
关键词：
Moxibustion;Point ST 21 (Liangmen);Point ST 36 (Zusanli);Stomach ulcer;Apoptosis;Cell proliferation
摘要：
OBJECTIVE: To observe the effect of moxa-burning heat stimulating acupoints of Liangmen (ST 21) and Zusanli (ST 36) on the proliferation and apoptosis signaling proteins in rats with stress-induced gastric ulcer. METHODS: Forty rats were randomly divided into four groups: negative control (NC), ulcer control (UC), acupoints of stomach meridian (ASM), and acupoints control (AC). The acute gastric ulcer model was established by bound and water immersion. Rats in NC and UC groups didn't receive any moxa-burning heat stimulating treatment, while rats in ASM and AC groups were treated with buringmoxa heat stimulating the acupoints of Liangmen (ST 21) and Zusanli (ST 36) and their controlled points, respectively. Rats in all groups were sacrificed after 12 consecutive days treatment. The ulcer index was evaluated by using Guth's method. The expression of tumor necrosis factor-alpha (TNF-α), apoptotic protease activating facter-1 (Apaf-1), Caspase-3, p21 activated kinase 1 (PAK1), extracellular regulated protein kinases 2 (ERK2), phosphorylated ERK2 (pERK2), phosphoinositide 3-kinase (PI3K) and RAC-alpha serine/threonine- protein kinase (Akt) in gastric mucosa was detected by enzyme linked immunosorbent assay (ELISA). RESULTS: Compared with UC group, the ulcer index of ASM and AC groups decreased, and the injured gastric mucosa was improved, the expression of TNF-α, Apaf-1 and Caspase-3 in gastric mucosa was significantly reduced (P < 0.05), while the expression of PAK1, ERK2, pERK2, PI3K and Akt in gastric mucosa was significantly increased (P < 0.05). And ASM showed better effect than AC group (P < 0.05). CONCLUSION: Moxa-burning Heat stimulating of Liangmen (ST 21) and Zusanli (ST 36) could promote the recovery of gastric mucosal lesion probably by inhibiting cell apoptosis and promoting cell proliferation in stress-induced gastric ulcer.
