期刊：
Frontiers in Pharmacology,2021年12:602543 ISSN：1663-9812
作者机构：
[Zhou, Qing; Zhan, Min; Bin, Dong-hua; Zhou, Xing] Hunan Univ Chinese Med, Surg Tradit Chinese Med, Affiliated Hosp 1, Changsha, Peoples R China.;[Zhang, Shi-ying; Li, Ling; Bin, Dong-hua] Hunan Univ Chinese Med, Coll Tradit Chinese Med, Changsha, Peoples R China.;[Zhang, Shi-ying] Shenzhen Luohu Peoples Hosp, Dept Tradit Chinese Med, Shenzhen, Peoples R China.;[Zhang, Shi-ying] Shenzhen Univ, Dept Tradit Chinese Med, Affiliated Hosp 3, Shenzhen, Peoples R China.;[Lu, Fang-guo; Li, Ying-qiu] Hunan Univ Chinese Med, Med Sch, Changsha, Peoples R China.
关键词：
Zhibai Dihuang Decoction;Ureaplasma urealyticum;Orchitis;Integrated pharmacological;bioinformatics;Chinese medicine;Herb medicine
摘要：
Background: Ureaplasma urealyticum (UU) infection is the most common cause of male infertility. Zhibai Dihuang Decoction (ZBDHD) can improve the rate of forwarding motility sperm, sperm deformity rate, seminal plasma zinc and refined berry sugar levels. Methods: The potential targets of ZBDHD are obtained from The Encyclopedia of Traditional Chinese Medicine (ETCM). Orchitis-related targets were collected from the Genecards and OMIM databases. The Cytoscape and the Database for Annotation, Visualization and Integrated Discovery (DAVID) were utilized to construct and analyzed the networks. Finally, a rat model of orchitis caused by UU infection was used to detect related indicators of mitochondrial energy metabolism using TUNEL apoptosis detection technology, loss cytometry, Real-Time Quantitative Reverse Transcription PCR (qRT-PCR) and Western Blot. Results: A total of 795 ZBDHD targets and 242 orchitis-related targets were obtained. The "ZBDHD- orchitis PPI network" was constructed and analyzed. ZBDHD can regulate signaling pathways and biological processes related to mitochondrial energy metabolism. The results of experimental studies have shown that ZBDHD maintains the integrity of sperm mitochondrial respiratory chain function by enhancing mitochondrial Na+-K+-ATPase and Ca2(+)-Mg2+-ATPase activities, promotes the synthesis of mitochondrial ATP, and improves sperm energy supply, thereby improving the motility, vitality and survival rate of sperm, and effectively improving the quality of semen in UU-infected rats (p < 0.05). Conclusion:This study discovered the multi-pathway mechanism of ZBDHD intervention in UU-induced orchitis through integrated pharmacological strategies, which provides a reference for further research on the mechanism of ZBDHD intervention in orchitis in the direction of mitochondrial energy metabolism.
作者机构：
[Li, Ling] Hunan Univ Chinese Med, Dept Expt Ctr Med Basic Teaching, Coll Tradit Chinese Med, Changsha, Hunan, Peoples R China.;[Lu, Fang-Guo; Ning, Yi; Wei, Ke] Hunan Univ Chinese Med, Dept Microbiol, Med Sch, Xueshi Rd 300, Changsha 410208, Hunan, Peoples R China.;[Wu, Tao; Zhao, Cheng; Hu, Jue] Hunan Univ Chinese Med, Grad Sch, Dept Postgrad, Changsha, Hunan, Peoples R China.;[He, Qing-Hu] Hunan Univ Chinese Med, Lab Integrated Chinese & Western Med, Coll Integrated Chinese & Western Med, Changsha, Hunan, Peoples R China.
通讯机构：
[Lu, Fang-Guo; He, Qing-Hu] H;Hunan Univ Chinese Med, Dept Microbiol, Med Sch, Xueshi Rd 300, Changsha 410208, Hunan, Peoples R China.;Hunan Univ Chinese Med, Lab Integrated Chinese & Western Med, Coll Integrated Chinese & Western Med, Changsha, Hunan, Peoples R China.
关键词：
Apoptosis;autophagy;influenza virus;macrophages;platycodin D
摘要：
Background: Platycodin D (PLD) comes from the main triterpenoid saponins in the dry roots of Platycodon grandiflorum, which has been used in traditional Chinese medicine in the treatment of respiratory disease. This study aimed to evaluate the therapeutic effects of PLD on mouse alveolar macrophages induced by influenza A virus (IAV) in vitro and to investigate the PLD of its action. Materials and Methods: Raw264.7 mouse alveolar macrophages were grown in culture and the Cell Counting Kit-8 assay determined cell viability. Cells were treated for 24 h with increasing doses of PLD (0, 5, 10, 20, 40, 60, 80, 100 mu M). The apoptosis was detected by Annexin V-FITC/PI and Tunel assay. Levels of interleukin (IL)-1 beta, IL-6 and tumor necrosis factor (TNF)-alpha of supernatants were assessed using enzyme-linked immunosorbent assay. The numbers of phagophores, autophagosomes, and autolysosomes and the ultrastructure of cells were assessed using transmission electron microscope assay. The relative protein expression of microtubule-associated protein light chain (LC) 3I, LC3II, Beclin-1, and B cell lymphoma (Bcl2) was evaluated by Western blotting. The localizations of Beclin-1 and Bcl2 were viewed with a fluorescence microscope. Results: PLD treatment resulted in significant dose-dependent inhibition of the growth of Raw264.7 cells with the half-maximal inhibitory concentration of 115.74 mu M at 24 h. After Raw264.7 cells infected by influenza virus, PLD (2.5, 5, and 10 mu M) can improve the activity of cells, reducing apoptosis of cells, decreasing the concentrations of IL-1 beta, IL-6, and TNF-alpha in the supernatant of cells, reducing autophagic injury of cells and regulating the protein expression of Beclin-1 and Bcl2. Conclusion: PLD could inhibit excessive apoptosis and autophagy of macrophage cells induced by IAV in a dose-dependent manner and then inhibits the secretion of inflammatory factors caused by influenza virus infection caused by apoptosis and autophagy and then ultimately regulate the pathogenesis of influenza virus.
摘要：
A graphene oxide (GO)-based fluorescent bioassay was developed to quantify agrA gene transcription (its mRNA) in methicillin-resistant Staphylococcus aureus (MRSA). This method is based on the use of Klenow fragment (KF)-assisted target recycling amplification and hybridization chain reaction (HCR). A triple complex was designed that contained a capture probe (CP), a trigger probe (TP), and a help probe (HP), which were partially complementary to one another. In the absence of the target, all the oligonucleotides labeled with carboxyfluorescein (FAM) are adsorbed onto the surface of GO by π-stacking interactions. This adsorption quenches the FAM signal. On the contrary, the target RNA causes the triple complex to disintegrate and initiates strand-displacement polymerization reaction (SDPR) and HCR in the presence of the appropriate raw materials, including the primer, KF, dNTPs, hairpin 1 (H1), and hairpin 2 (H2), generating double-stranded DNA (dsDNA) products. These dsDNA products are repelled by GO and produce strong fluorescence, measured at excitation/emission wavelengths of 480/514nm. The fluorescent signal is greatly amplified by SYBR Green I (SGI) due to the synergistic effect of dsDNA-SGI. The target was assayed with this method at concentrations in the range 10 fM to 100 pM, and the detection limit (LOD) was 10 fM. This method also displayed good applicability in the analysis of real samples. It provides a new way of monitoring biofilm formation and studying the mechanisms of drug actions. Graphical abstract Schematic representation of the graphene oxide-based fluorescent bioassay for agrA gene transcription in methicillin-resistant Staphylococcus aureus by using strand-displacement polymerization recycling and hybridization chain reaction.
作者机构：
[Chen-Yang Zhang; Ling Li; Xiao-Nan Wu] Hunan University of Traditional Chinese Medicine, Changsha, 410208, China.;[Zhao-Yong Li; Shao-Feng Yang] The First Hospital of Hunan University of Chinese Medicine, Changsha, 410007, China.
通讯机构：
[Shao-Feng Yang] T;The First Hospital of Hunan University of Chinese Medicine, Changsha, 410007, China.
摘要：
Objective: To systematically evaluate the cllinical efficacy of Duhuo Jisheng decoction (DHJSD) combined with non-surgical therapy on lumbar disc herniation (LDH). Methods: China Science and Technology Journal Database (VIP), Chinese National Knowledge Infrastructure (CNKI), WanFang Data, PubMed were searched and eligible randomized controlled clinical trials (RCTs) were included. Two reviewers evaluated the quality of the included RCTs and extracted data independently. Then, data analysis was performed with RevMetn 4.2 software. Results: A total of 18 RCTs were enrolled, including 1682 patients. Meta-analysis results showed that the overall response rate of DHJSD combined with non-operative therapy group (treatment group) in the treatment of LDH was better than that of the non-operative therapy group (control group) [OR = 4.29, 95% CI (3.19, 5.76), P < 0.001]. JOA score of the treatment group was significantly higher than that of control group [OR = 3.96, 95% CI (3.4, 4.52), P < 0.001]. VAS score was dramatically lower in the treatment group than the control group [OR = -1.58, 95% CI (-1.97, -1.18), P < 0.001]. Conclusion: The clinical efficacy of DHJSD combined with non-surgical therapy is better than that of non-surgical therapy alone. However, due to the low quality of the included literatures, large-scale and high-quality RCTs are still needed for further confirmation.
作者机构：
[Jia-Wang H.; Ping Y.; XIE Xi; Meng-Chen Z.; Jian W.] College of Integrated Traditional Chinese and Western Medicine, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;[Ling L.; Can L.] College of Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;[Ping-An C.] Medical College, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China
通讯机构：
[Li Ling] C;College of Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha, Hunan, 410208, China
关键词：
16SrDNA;Fu Zi Li Zhong Decoction (FZLZD) Gastric ulcer;Intestinal flora diversity;Occludin;ZO-1
