作者机构：
[Li, Ling] Hunan Univ Chinese Med, Dept Expt Ctr Med Basic Teaching, Coll Tradit Chinese Med, Changsha, Hunan, Peoples R China.;[Lu, Fang-Guo; Ning, Yi; Wei, Ke; Lu, FG] Hunan Univ Chinese Med, Dept Microbiol, Med Sch, Xueshi Rd 300, Changsha 410208, Hunan, Peoples R China.;[Wu, Tao; Zhao, Cheng; Hu, Jue] Hunan Univ Chinese Med, Grad Sch, Dept Postgrad, Changsha, Hunan, Peoples R China.;[He, Qing-Hu] Hunan Univ Chinese Med, Lab Integrated Chinese & Western Med, Coll Integrated Chinese & Western Med, Changsha, Hunan, Peoples R China.
通讯机构：
[He, QH ; Lu, FG ] H;Hunan Univ Chinese Med, Dept Microbiol, Med Sch, Xueshi Rd 300, Changsha 410208, Hunan, Peoples R China.;Hunan Univ Chinese Med, Lab Integrated Chinese & Western Med, Coll Integrated Chinese & Western Med, Changsha, Hunan, Peoples R China.
关键词：
Apoptosis;autophagy;influenza virus;macrophages;platycodin D
摘要：
Background: Platycodin D (PLD) comes from the main triterpenoid saponins in the dry roots of Platycodon grandiflorum, which has been used in traditional Chinese medicine in the treatment of respiratory disease. This study aimed to evaluate the therapeutic effects of PLD on mouse alveolar macrophages induced by influenza A virus (IAV) in vitro and to investigate the PLD of its action. Materials and Methods: Raw264.7 mouse alveolar macrophages were grown in culture and the Cell Counting Kit-8 assay determined cell viability. Cells were treated for 24 h with increasing doses of PLD (0, 5, 10, 20, 40, 60, 80, 100 mu M). The apoptosis was detected by Annexin V-FITC/PI and Tunel assay. Levels of interleukin (IL)-1 beta, IL-6 and tumor necrosis factor (TNF)-alpha of supernatants were assessed using enzyme-linked immunosorbent assay. The numbers of phagophores, autophagosomes, and autolysosomes and the ultrastructure of cells were assessed using transmission electron microscope assay. The relative protein expression of microtubule-associated protein light chain (LC) 3I, LC3II, Beclin-1, and B cell lymphoma (Bcl2) was evaluated by Western blotting. The localizations of Beclin-1 and Bcl2 were viewed with a fluorescence microscope. Results: PLD treatment resulted in significant dose-dependent inhibition of the growth of Raw264.7 cells with the half-maximal inhibitory concentration of 115.74 mu M at 24 h. After Raw264.7 cells infected by influenza virus, PLD (2.5, 5, and 10 mu M) can improve the activity of cells, reducing apoptosis of cells, decreasing the concentrations of IL-1 beta, IL-6, and TNF-alpha in the supernatant of cells, reducing autophagic injury of cells and regulating the protein expression of Beclin-1 and Bcl2. Conclusion: PLD could inhibit excessive apoptosis and autophagy of macrophage cells induced by IAV in a dose-dependent manner and then inhibits the secretion of inflammatory factors caused by influenza virus infection caused by apoptosis and autophagy and then ultimately regulate the pathogenesis of influenza virus.
作者：
ChenYang Zhang;Ling Li;ZhaoYong Li;XiaoNan Wu;ShaoFeng Yang
期刊：
临床研究通讯,2019年2(3):115-124 ISSN：2815-7176
通讯作者：
Shao-Feng Yang
作者机构：
[ChenYang Zhang; Ling Li; XiaoNan Wu] Hunan University of Traditional Chinese Medicine, Changsha, 410208, China.;[ZhaoYong Li; ShaoFeng Yang] The First Hospital of Hunan University of Chinese Medicine, Changsha, 410007, China.
通讯机构：
[Shao-Feng Yang] T;The First Hospital of Hunan University of Chinese Medicine, Changsha, 410007, China.
摘要：
Objective: To systematically evaluate the cllinical efficacy of Duhuo Jisheng decoction (DHJSD) combined with non-surgical therapy on lumbar disc herniation (LDH). Methods: China Science and Technology Journal Database (VIP), Chinese National Knowledge Infrastructure (CNKI), WanFang Data, PubMed were searched and eligible randomized controlled clinical trials (RCTs) were included. Two reviewers evaluated the quality of the included RCTs and extracted data independently. Then, data analysis was performed with RevMetn 4.2 software. Results: A total of 18 RCTs were enrolled, including 1682 patients. Meta-analysis results showed that the overall response rate of DHJSD combined with non-operative therapy group (treatment group) in the treatment of LDH was better than that of the non-operative therapy group (control group) [OR = 4.29, 95% CI (3.19, 5.76), P < 0.001]. JOA score of the treatment group was significantly higher than that of control group [OR = 3.96, 95% CI (3.4, 4.52), P < 0.001]. VAS score was dramatically lower in the treatment group than the control group [OR = -1.58, 95% CI (-1.97, -1.18), P < 0.001]. Conclusion: The clinical efficacy of DHJSD combined with non-surgical therapy is better than that of non-surgical therapy alone. However, due to the low quality of the included literatures, large-scale and high-quality RCTs are still needed for further confirmation.
作者机构：
[Jia-Wang H.; Ping Y.; Xie Xi; Meng-Chen Z.; Jian W.] College of Integrated Traditional Chinese and Western Medicine, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;[Ling L.; Can L.] College of Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;[Ping-An C.] Medical College, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China
通讯机构：
[Li Ling] C;College of Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha, Hunan, 410208, China
关键词：
16SrDNA;Fu Zi Li Zhong Decoction (FZLZD) Gastric ulcer;Intestinal flora diversity;Occludin;ZO-1
