期刊：
Evidence-Based Complementary and Alternative Medicine,2020年2020 ISSN：1741-427X
通讯作者：
Peng, Qinghua;Cai, Xiong
作者机构：
[Chen, Xiaojuan; Chen, Xinyi; Peng, Qinghua; Lin, Ye; Song, Houpan] Hunan Univ Chinese Med, Inst Tradit Chinese Med Diagnost, Changsha, Hunan, Peoples R China.;[Chen, Xiaojuan; Chen, Xinyi; Yu, Rong; Lin, Ye; Zeng, Meiyan; Song, Houpan] Hunan Univ Chinese Med, Coll Tradit Chinese Med, Changsha, Hunan, Peoples R China.;[Peng, Jun] Hunan Univ Chinese Med, Affiliated Hosp 1, Dept Ophthalmol, Changsha, Hunan, Peoples R China.;[Cai, Xiong] Hunan Univ Chinese Med, Chinese Med, Inst Innovat & Appl Res, Changsha, Hunan, Peoples R China.
通讯机构：
[Peng, Qinghua; Cai, Xiong] H;Hunan Univ Chinese Med, Inst Tradit Chinese Med Diagnost, Changsha, Hunan, Peoples R China.;Hunan Univ Chinese Med, Chinese Med, Inst Innovat & Appl Res, Changsha, Hunan, Peoples R China.
摘要：
Background. Administration of nonsteroidal anti-inflammatory drugs (NSAIDs) often causes small intestinal ulcers in patients, but few effective drugs are currently available to manage such serious adverse events of NSAIDs. Li-Zhong decoction (LZD), a well-known traditional Chinese medicine (TCM) formula, is commonly prescribed for treatment of gastrointestinal diseases. The present study aimed to investigate the anti-ulcerogenic activity of LZD on indomethacin- (IND-) induced duodenal ulcer in rats. Mechanistic studies of action of LZD were focused on involvement of TLR-2/MyD88 signaling pathway. Methods. Fifty male Sprague-Dawley (SD) rats were randomly and evenly divided into five groups: normal control, ulcer control (IND, 25 mg/kg), IND + esomeprazole (ESO, 4.17 mg/kg), and IND + low and high doses of LZD (3.75 and 7.50 g/kg). Macroscopic and histopathological examinations were performed for evaluation of ulcer index (UI), curative index (CI), and microscopic score (MS). Levels of duodenal inflammatory biomarkers and cytoprotective mediators including interleukin-4 (IL-4), IL-10, tumor necrosis factor-alpha (TNF-alpha), and prostaglandin E-2 (PGE(2)) were measured by ELISA. Expression levels of TLR-2 and MyD88 mRNA were assessed by qRT-PCR. The expression and distribution of TLR-2 and MyD88 proteins were analyzed by western blot and immunohistochemistry, respectively. Results. Gross and microscopic examinations of the IND-treated rats revealed severe duodenal hemorrhagic necrosis, inflammatory infiltration, villus destruction, and crypt abscess, while LZD-treated rats manifested these pathological events to a markedly lesser degree. LZD significantly decreased UI and MS, increased CI, preserved the integrity of the villus and crypt, and normalized the tissue architecture of the duodenum of rats. The elevated TNF-alpha levels in the IND-treated rats were markedly diminished in the LZD-treated rats, while lower levels of IL-4, IL-10, and PGE(2) observed in IND-treated rats were significantly increased in LZD-treated rats. Interestingly, improvement of immune function in duodenal mucosa by reduction of mRNA and protein expression levels of TLR-2 and MyD88 was also observed in rats treated with LZD. Consistently, immunohistochemical analyses revealed a lower co-localization of TLR-2 and MyD88 proteins in the duodenal mucosa of LZD-treated rats as compared to the IND-induced rats. Conclusions. Our data demonstrate that LZD protects the duodenal mucosa from IND-caused lesions, which is at least partially attributable to the interaction of its potential cytoprotective and anti-inflammatory mechanisms together with enhancement of the mucosal immunity through TLR-2/MyD88 signaling pathway.
摘要：
目的:观察密蒙花颗粒剂对干眼患者泪液白细胞介素-1β(Interleukin-1β,IL-1β)和白细胞介素-18(Interleukin-18,IL-18)表达的影响。方法:将70位干眼患者按随机数字表法平均分为2组,对照组以玻璃酸钠滴眼液治疗,治疗组在对照组的基础上口服密蒙花颗粒剂。治疗4周后,随访4个月,观察两组患者干眼症状评分、泪膜破裂时间(break up time,BUT)、泪液分泌试验(Schirmer I test,SIT)、角膜荧光素钠染色评分(fluorescent,FL)的情况,ELISA法检测患者泪液中IL-1β、IL-18的表达。结果:治疗组治疗后2周、4周干眼症状评分和FL较治疗前降低(P<0.05),SIT和BUT较治疗前升高(P<0.05);对照组治疗后2周、4周干眼症状评分和FL较治疗前降低(P<0.05),SIT和BUT较治疗前升高(P<0.05)。治疗组治疗后4周干眼症状评分和FL较治疗后2周降低(P<0.05),SIT和BUT较治疗后2周升高(P<0.05);对照组治疗后4周干眼症状评分和FL较治疗后2周降低(P<0.05),SIT和BUT较治疗后2周升高(P<0.05)。治疗后2周、4周两组干眼症状评分、SIT、BUT及FL相比,差异均有统计学意义(P<0.05)。治疗组治疗后2周、4周泪液IL-1β和IL-18表达较治疗前降低(P<0.05);对照组治疗后2周、4周泪液IL-1β和IL-18表达较治疗前降低(P<0.05)。治疗组治疗后4周泪液IL-1β和IL-18表达较治疗后2周降低(P<0.05);对照组治疗后4周泪液IL-1β和IL-18表达较治疗后2周降低(P<0.05)。治疗后2周、4周两组泪液IL-1β和IL-18表达相比,差异均有统计学意义(P<0.05)。结论:密蒙花颗粒剂对干眼患者泪液中IL-1β、IL-18的表达具有抑制作用,这可能是密蒙花颗粒剂治疗干眼的分子生物学机制。
摘要：
Objective To observe the effects of Sanxue Mingmu Tablets (散血明目片) combined with conbercept on patients with wet age-related macular degeneration (wAMD).Methods A total of 70 patients with wAMD were randomly divided into a treatment group with 35 cases (51 eyes) and a control group with 35 cases (50 eyes). The control group was treated with intravitreal injection of conbercept 0.05 ml once a month for three times. The treatment group was additionally treated with oral Sanxue Mingmu Tablets 2.4 g each time, three times a day for three months. The best corrected visual acuity (BCVA), electroretinogram (ERG) amplitude and latency of a wave and 6 wave of the 2 groups were observed before treatment and 1 month, 2 months, 3 months after treatment. Ultrasound was used to detect the peak systolic short-velocity (PSV), end-diastolic flow (EDV), and resistance index (RI) of the posterior ciliary short arteries in the 2 groups of patients. Optical coherence tomography (OCT) was used to detect the macular foveal thickness (CMT). The postoperative complications and the recurrence of 6 months follow-up after treatment were observed.Results Compared with the previous time point within group, the BCVA in both groups was significantly decreased after 1, 2, and 3 months of treatment (P<0.05). When the amplitudes of a wave and b wave, RI, and CMT were compared at all time point in the 2 groups, the differences were statistically significant (P<0.05). There was a statistically significant difference in the EPG a-wave, b-wave amplitude, RI, and CMT between groups after 1, 2, and 3 months of treatment (P<0.05). Compared with in group before treatment, except for PSV after 1 month in the control group, there was a statistically significant difference in PSV and EDV after treatment for 1, 2, and 3 months between groups (P<0.05). The differences between PSV at 2 and 3 months after treatment and EDV at 1, 2, and 3 months after treatment were statistically significant (P<0.05). Conjunctival hyperemia occurred in 1 eye in the treatment group and 2 eyes in the control group after surgery. There was no significant difference in complications between groups (P>0.05). There were 4 cases (5 eyes) relapsed in the treatment group, with a recurrence rate of 11.11%, and 11 patients (15 eyes) relapsed in the control group, with a recurrence rate of 34.09%. The recurrence rate of the treatment group was lower than that of the control group.Conclusion Sanxue Mingmu Tablets combined with Combercept ophthalmic injection can promote the absorption of retinal exudation and bleeding after wAMD, improve vision, and have a good effect on wAMD.
作者机构：
[Zeng Z.-C.] Hunan University of Chinese Medicine, Changsha, 410208, China;[彭俊] Department of Ophthalmology, First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, 410007, China;[蒋鹏飞; 黎冬冬; 彭清华] Hunan University of Chinese Medicine, Changsha, 410208, China, Department of Ophthalmology, First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, 410007, China
通讯机构：
[Peng, Q.-H.] H;Hunan University of Chinese Medicine, Changsha, China
摘要：
ETHNOPHARMACOLOGICAL RELEVANCE: Li-Zhong-Tang (LZT) is a well-known Chinese herbal formulation first described in one of traditional Chinese medicine (TCM) scriptures, Treatise on Febrile Diseases. LZT has been commonly prescribed for the treatment of various gastrointestinal diseases for over 1800 years, and has demonstrated pronounced therapeutic effects on patients with gastric ulcers. AIM OF THE STUDY: The present study aimed to scientifically evaluate protective effects of LZT on indomethacin (IND)-induced gastric injury in rats and to elucidate whether LZT exerts its gastro-protective effects via enhancing mucosal immunity by regulating TLR-2/MyD88 signaling pathway. MATERIAL AND METHODS: Gastric ulcers were induced in male Sprague-Dawley (SD) rats with a single oral dose of 150mg/kg IND. Ulcer index (UI) and curative index (CI) were evaluated. Histopathological examinations were performed and microscopic score (MS) was macroscopically calculated. The volume of gastric juice, free acidity, total acidity, and gastric pH was measured. The gastroprotective and inflammatory biomarkers including levels of nitric oxide (NO), tumor necrosis factor-α (TNF-α), prostaglandin E(2) (PGE(2)), and malondialdehyde (MDA) were determined. Expression levels of TLR-2 and MyD88 mRNA were assessed by qRT-PCR. The expression, distribution, and co-localization of TLR-2 and MyD88 protein were determined by Western blot, immunohistochemistry, and immunofluorescence, respectively. RESULTS: Induction of gastric ulcers in rats resulted in very significantly increased UI and elevated volume and acidity of gastric juice, which were markedly attenuated by LZT treatment. Microscopic examinations of the IND-induced gastric ulcers revealed severe gastric hemorrhagic necrosis, submucosal edema, and destruction of epithelial cells, which were significantly attenuated in LZT-treated rats. Moreover, treatment with LZT remarkably increased gastric mucosal levels of PGE(2) and NO, and lowered highly elevated levels of TNF-α and MDA in gastric ulcerative rats. Mechanistically, LZT inhibited mRNA and protein expression of TLR-2 and MyD88 and enhanced immune function in gastric mucosa. Immunohistochemical analyses and immunofluorescent detection further confirmed a markedly decreased co-localization of TLR-2 and MyD88 protein in the gastric mucosa of LZT-treated rats as compared to that of gastric ulcerative rats. CONCLUSIONS: These findings indicate that LZT alleviates serious gastric mucosal ulcerations induced by IND. Protective effects of LZT on gastric ulcers are believed to be associated with the intensification of the anti-oxidative defense system, mitigation of proinflammatory cytokines, stimulation of the production of cytoprotective mediators, and improvement of the mucosal immunity through TLR-2/MyD88 signaling pathway.
